Digital Commons Network^™

Examining The Understanding Of Inquiry-Based Learning And Teaching Among Undergraduate Teachers And Students, Maren Hudson

Electronic Theses and Dissertations

One of the main aims of inquiry is to engage students as active, not passive, participants in science. The purpose of this study is to describe science educators’ and students’ views about inquiry-based instruction in order to better understand and improve implementation of evidence-based teaching strategies. Inquiry-based techniques have been shown to improve student understanding of scientific concepts, yet, there continue to be challenges in implementing these techniques. This research project utilizes Q Methodology, a research method that captures both common and disparate measures of subjectivity, to identify commonalities and defining viewpoints about inquiry-based teaching and learning. Three significantly different …

Study Of 2,5-Diaminoimidazolone, A Mutagenic Product Of Oxidation Of Guanine In Dna, Hannah Catherine J. Pollard

Electronic Theses and Dissertations

2,5-diaminoimidazolone (Iz) is an important product of a 4-electron oxidation of guanine. The present research focuses on the mechanisms of formation of Iz via pathways initiated by guanine oxidation by one-electron oxidants (OEOs) generated by X-ray radiolysis in aqueous solutions. The kinetics of formation and yields of Iz in reactions of native highly polymerized DNA with different OEOs have been compared using an HPLC-based quantitative analysis of low-molecular products generated from the reaction of DNA-bound Iz with primary amines. Mechanisms of Iz formation in DNA have been investigated including oxygen and superoxide dependence as well as the hypothesis that 8-oxo-G, …

The Algz/R Two-Component System Is Responsible For Attenuation Of Virulence In Pseudomonas Aeruginosa, Danielle A. Williams

Electronic Theses and Dissertations

Pseudomonas aeruginosa is an important opportunistic pathogen. Many P. aeruginosa virulence factors are regulated by the AlgZ/R two component system. AlgZ is the sensor histidine kinase which phosphorylates AlgR, the response regulator. AlgR activates transcription of different gene targets based upon its phosphorylation state. The genes that encode AlgZ and AlgR are transcribed in an operon. While regulation of algR expression has been well studied, regulation of algZ expression has not. Using a pilW mutant in concert with algZTF-lacZ transcriptional fusion, we conducted a transposon mutagenesis to identify algZ regulators. We identified an unknown autoregulatory loop. The type …

Predicting And Promoting Health-Related Quality Of Life For Parents Of Children With Autism: A Mixed-Methods Approach, Laura Delustro

Electronic Theses and Dissertations

The present study used a mixed methodology approach to understand factors that predict and promote health related quality of life (HRQOL) for parents of youth with Autism Spectrum Disorders (ASD). The first component of the study used quantitative analyses to determine the extent to which study variables predict HRQOL for parents of youth with ASD (N = 365). Findings show that HRQOL is positively impacted by increased total annual household income, increased age of the child with ASD, male gender, and tangible support. HRQOL was negatively impacted by increased age of the parent, increased parental perceptions of burden, and …

Role Of Cannabinoid Receptor Type 2 (Cb2) In Late Stage Atherosclerosis, Makenzie Fulmer

Electronic Theses and Dissertations

Atherosclerosis is a chronic inflammatory disorder of medium and large vessels. Immune signaling and dyslipidemia are two of several processes which influence lesion development in atherosclerosis. Cannabinoids, such as those found in marijuana, exert their effects through two cannabinoid receptors, CB1 and CB2. Recent studies using CB2 knockout mice and CB2-selective ligands have shed light on a protective role of CB2 in early stages of atherosclerosis. However, the role of CB2 in advanced stages of atherosclerosis remains unclear. To determine if CB2 plays a role in advanced atherosclerotic lesion composition and progression, we investigated the effects of systemic CB2 gene …

The Relationship Of School-Community Partnerships With Act Benchmark Scores In Rural Tennessee Schools, Kari Eubanks

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if a relationship existed between the quality of school-community partnerships and the average score of each ACT subtest for rural Tennessee high school students. Specifically, the researcher examined the following school-community partnerships: business partnerships, university partnerships, service learning partnerships, school-linked service integration, and faith-based partnerships (Sanders, 2006). Administrators from 62 rural Tennessee high schools rated the partnerships present in their schools using the Improving Community Partnership Quality rating scale developed by Sanders (2006). Each of these ratings was compared to the participating school’s mean score for each ACT benchmark to determine …

Estimating Wildlife Biodiversity Of Pine Plantation Edges Of Contrasting Rotational Stages: A Case Study, Joshua Perry Harris

Electronic Theses and Dissertations

ESTIMATING WILDLIFE BIODIVERSITY OF PINE PLANTATION EDGES OF CONTRASTING ROTATIONAL STAGES: A CASE STUDY

By: Joshua Harris, Dr. Daniel Scognamillo, Dr. Gary Kronrad, and Dr. Jeremy Stovall

Abstract

Wildlife biodiversity was estimated amongst three loblolly pine (Pinus taeda) intra-plantation edge types of contrasting rotational phases in a two-year case study. Three structurally varied rotational phases that occur throughout plantation rotations combine to make three different edges: A= pre-thinned - post-thinned, B= pre-thinned - pre-clearcut, and C= post-thinned - pre-clearcut. For two summer seasons (May-August 2015-2016), invertebrates, birds, medium mammals, and small mammals were sampled in each edge type. …

Spatially Explicit Model Of Areas Between Suitable Black Bear Habitat In East Texas And Black Bear Populations In Louisiana, Arkansas, And Oklahoma, Caitlin M. Glymph, Christopher Comer, Daniel Scognamillo, Daniel Unger, Yanli Zhang

Electronic Theses and Dissertations

Although black bears (Ursus americanus, Ursus americanus luteolus) were once found throughout the south-central United States, unregulated harvest and habitat loss resulted in severe range retractions and by the beginning of the twentieth century populations in Oklahoma, Louisiana, Texas and Arkansas were nearing extirpation. In response to these losses, translocation programs were initiated in Arkansas (1958-1968 & 2000-2006) and Louisiana (1964-1967 & 2001-2009). These programs successfully restored bears to portions of Louisiana and Arkansas, and, as populations in Arkansas began dispersing, to Oklahoma. In contrast, east Texas remains unoccupied despite the existence of suitable habitat in the region.

To facilitate …

Evaluating Mine Soil Prepared By Scraper Pans For Herbaceous Plants And Pine Seedlings At An East Texas Lignite Surface Mine, Hannah Z. Angel

Electronic Theses and Dissertations

Since 1974, Luminant Mining Company, LLC (Luminant) has planted over 38.7 million trees on its reclaimed lignite surface mine operations in Texas. For decades, the use of improved reclamation techniques on Luminant’s mined lands have resulted in over 31,160 ha reclaimed to forests, wildlife habitat, and pastures with productivity levels similar to those found on unmined lands. The development of new reclamation methodologies offers opportunities to further improve productivity of planted trees at Luminant’s Martin Lake Oak Hill Mine in east Texas. The conventional haulback or ‘truck shovel’ reclamation method uses haul trucks for the selective transport and placement of …

The Moderating Effects Of Positive Religious/Spiritual Coping On Teachers’ Pain And Stress, Dawn Green

Electronic Theses and Dissertations

The objective of this internet-based survey was to investigate the effects of positive religious or spiritual coping strategies on teachers’ chronic pain reports. Teachers in the United States may represent a vulnerable group due to a high prevalence of risk factors for chronic pain conditions. Teachers have been identified to experience high stress (Johnson, et al., 2005; Kyriacou, 2001) and report poor job satisfaction (Wang, Hall, & Rahimi, 2015), which are associated with development of chronic pain conditions (Kopec & Sayre, 2004). Religious coping strategies have been associated with beneficial associations with stress and health (Reutter & Bigatti, 2014). The …

An Examination Of The Relationship Between Ethnic Identity Development, Cognitive Complexity, Differentiation Of Self, And Reports Of Racial And Ethnic Microaggressions, Deshae C. Gatti

Electronic Theses and Dissertations

Although individuals may not engage in racism consciously due to social undesirability, they may have biases that manifest in more subtle and unconscious ways. Research has found that people of color experiencing different racial and ethnic microaggressions in their everyday lives are subject to pervasive and negative impacts on their mental health, though little is known about the influence of one’s ethnic identity formation, affective processes, and cognitive processes. A multiple regression analysis was conducted to examine the degree to which ethnic identity development, cognitive complexity, and differentiation of self were associated reported experiences of microaggressions. The analysis indicated that …

Pinching And Spacing Effects On Cut Sunflower (Helianthus Annuus) Production In East Texas, Rebecca B. Burnett

Electronic Theses and Dissertations

Three experiments evaluated pinching and spacing on Helianthus annuus in East Texas to determine their effects on growth and development. Experiment 1 was designed to determine the effects of pinching nodes 1, 2, 3, or 4 on ‘Pro Cut Gold’ sunflowers. Results from experiment 1 showed non-pinched plants produced marketable stem lengths and flower sizes, while pinched plants’ stem length, stem diameter, flower diameter, and disk diameter decreased compared to the non-pinched plants. The objective for experiment 2 was to evaluate pinching and spacing treatments on ‘Pro Cut Gold’ sunflowers. All spacings for non-pinched plants in experiment 2 produced marketable …

The Application Of Proper Orthogonal Decomposition To Numerically Modeled And Measured Ocean Surface Wave Fields Remotely Sensed By Radar, Andrew Joseph Kammerer

Electronic Theses and Dissertations

Phase-resolved ocean surface wave elevation maps provide important information for many scientific research areas (e.g., rogue waves, wave-current interactions, and wave evolution/growth) as well as for commercial and defense applications (e.g., naval and shipping operations). To produce these maps, measurements in both time and space are necessary. While conventional wave sensing techniques are limited spatially, marine radar has proven to be a complex yet promising remote sensing tool capable of providing both temporal and spatial wave measurements. The radar return from the sea surface is complex because it contains contributions from many sources only part of which provide information about …

Quality And Yield Responses Of Seven Warm Season Forage Grasses To Partial Shading In A Simulated Silvopasture In East Texas, Jodi E. Hill

Electronic Theses and Dissertations

The goal of this project was to evaluate growth and nutritional characteristics of seven forages, including various warm season native grasses, grown under simulated partial shading (50%) typical of a loblolly pine silvopastoral system in East Texas. In order to meet the overall objective, slatted shade structures were constructed that simulated the quantity, quality, and overall light regime found beneath loblolly pine stands arranged for silvopasture. The forages selected for the study included ‘Tifton 9’ bahiagrass (Paspalum notatum), ‘Tifton 85’ bermudagrass (Cynodon dactylon), ‘Alamo’ switchgrass (Panicum virgatum), ‘Kaw’ Big Bluestem (Andropogon gerardii), ‘Americus’ …

Exploring A Multifactorial, Clinical Model Of Thought Disorder : Application Of A Dimensional, Transdiagnostic Approach., Mara Ann Hart

Electronic Theses and Dissertations

Background: Bleuler saw thought disorder as the core defining feature of psychotic phenomena, reflective of the “splitting of the psychic functions” that occurred when, in the process of thinking, one’s ideas and feelings disconnect, becoming fragmented and competing functions. Unfortunately, interest in thought disorder as the conceptual core of psychosis was lost with rise of the modern DSM system, paralleling the shift towards a more simplistic, categorical way of defining psychiatric disorders. Aims: This study examined thought disorder from a dimensional perspective, with the aim of disentangling qualitative heterogeneity and diverse sources of influence. Analyses were based on …

The Dlk1-Meg3 Locus In Malignant Cells Of Proposed Primordial Germ Cell Origins., Zachariah Payne Sellers

Electronic Theses and Dissertations

Primordial germ cells (PGCs) are hypothesized to deposit hematopoietic stem cells (HSCs) along their migration route through the embryo during the early stages of embryogenesis. PGCs also undergo global chromatin remodeling, including the erasure and reestablishment of genomic imprints, during this migration. While PGCs do not spontaneously form teratomas, their malignant development into germ cell tumors (GCTs) in vivo is often accompanied by the retention of hypomethylation at the IGF2-H19 imprinting control differentially methylated region (DMR). Previous studies in bimaternal embryos determined that proper genomic imprinting at two paternally imprinted loci was necessary for their growth and development: Igf2-H19 and …

Porphyromonas Gingivalis Infection In Gestational Diabetes Mellitus And Survival In Tobacco Smokers., Himabindu Gogeneni

Electronic Theses and Dissertations

This thesis contains a clinical project and distinct basic science project. Gestational diabetes mellitus occurs in 4% of pregnancies and increases the risk of birth defects, pre-term birth, and miscarriage. Gingivitis during pregnancy also increases the risk for poor pregnancy outcome. Gingivitis is a bacterial-induced disease, and specific plaque pathogens have been associated with systemic sequelae to periodontal inflammation. Therefore, we set out to monitor oral infection with three key periodontopathogens (Porphyromonas gingivalis, Filifactor alocis, and Treponema denticola) and the systemic inflammatory burden [C-reactive protein (CRP) in pregnant women with and without gingivitis and gestational diabetes. Gingivitis …

Applications Of Polarized Metallic Nanostructures., Jasmin Beharic

Electronic Theses and Dissertations

Gold nanostructures exhibit technologically useful properties when they are polarized in an electric field. In two projects we explore instances where the polarized metal can be used in real world applications. The first project involves gold nanoparticles (GNP) for use in light actuated microelectromechanical systems (MEMS) applications. Although the GNPs were originally designed for volumetric heating in biomedical applications, we treat them as a thin film coating, opening the door for these particles to be used in MEMS applications. This work characterizes the thermal properties of gold nanoparticles on surfaces for spatially-targeted thermal actuation in MEMS systems. The second project …

Ubqln1 : A Multi-Domain Protein With Multiple Functions., Zimple Kurlawala

Electronic Theses and Dissertations

There are 5 Ubiquilin proteins (UBQLN1-4, UBQLN-L), which are evolutionarily conserved and structurally similar. UBQLN proteins have 3 functional domains: N-terminal ubiquitin-like domain (UBL), C-terminal ubiquitin-associated domain (UBA) and STI chaperone-like regions in the middle. Alterations in UBQLN1 gene have been detected in a variety of disorders including Alzheimer’s disease, Amyotropic Lateral Sclerosis and lung cancer. UBQLN1 has been largely studied in neurodegenerative disorders in the context of protein quality control. Several studies have hypothesized that the UBA domain of UBQLN1 binds to poly-ubiquitin chains of substrate and shuttles it to the proteasome via its UBL domain for degradation. UBQLN1 …

Targeting Fas Pathway As An Effective Means Of Inducing Tolerance To Pancreatic Islets., Kyle Blake Woodward

Electronic Theses and Dissertations

Signaling through Fas/FasL is critical to immune homeostasis and tolerance to self-antigens. SA-FasL is a chimeric protein of FasL and streptavidin. SA-FasL exists as oligomers with potent apoptotic function on Fas expressing immune cells and tightly binds to biotinylated surfaces. Islet grafts engineered to transiently display SA-FasL on their surface established tolerance in allogeneic recipients with a short course of rapamycin. We hypothesized that SA-FasL on the islet allograft will induce apoptosis in alloreactive T effector (Teff) cells and phagocytes clearing apoptotic bodies will produce tolerogenic molecules, such as TGF-β, that will lead to the generation and/or expansion of Treg …

The Relationship Between Trauma And Health-Related Quality Of Life In Lung Cancer Patients : The Potential Protective Role Of Mindfulness., Rene Bayley-Veloso

Electronic Theses and Dissertations

Lung cancer is the most common malignant disease worldwide, and the rapid decline in functioning due to the often-later stage diagnosis can strongly impact a patient’s health-related quality of life (HRQOL). Palliative care, with the aim of improving later-stage HRQOL, is often a main goal of treatment. Compared to other cancer types, patients with lung cancer experience the greatest amount of psychological distress during and after treatment. Prior trauma exposure, and the potentially traumatic nature of the cancer experience, can further complicate HRQOL. Mindfulness, an innate or acquired capacity for sustaining attention in the present moment with qualities of self-warmth …

Attending To Systemic Racism : Advancing Public Health's Approach To Youth Violence Prevention., Billie Faith Castle

Electronic Theses and Dissertations

This dissertation examines how the field of public health addresses the impact of systemic racism on health and how that informs public health’s approach to youth violence prevention. Beginning with an overview of youth violence, it breaks down the concepts of race and racism and how they are addressed within the science. It also reviews concepts that contribute to risk and protective factors of youth violence. The dissertation is written from a Critical Race Theory approach, argues that the social environment contributes to why youth violence is pervasive in certain neighborhoods, and promotes action from a macro-level approach. Seven chapters …

Fabrication And Characterization Of A Mcro/Nanofluidic Platform For Electroporation., Hanwen Yuan

Electronic Theses and Dissertations

For traditional electroporation devices, there are a number of problems associated with these devices such as insufficient understanding of its theoretical mechanism, low cell viability, inadequate electroporation efficiency, excess voltage applied to generate required electric field due to the large size of these devices and sample contamination. Although newly developed microfluidic electroporation devices have solved most of the above existing problems in traditional bulk electroporation devices, they appear to lack the ability to control the precise dose of biomolecules or genes transfecting into cells and, from a manufacturing perspective, the fabrication methods do not enable repeatable production of such devices …

Regulation Of Haplid Phenotypes In Ustilago Maydis By Ammonium Transporters And Components Of The B Mating Locus., Rena Margaret Wallen

Electronic Theses and Dissertations

Fungi that can switch from budding to a filamentous infectious state have evolved mating type loci. Ustilago maydis, the maize pathogen, must mate with compatible partners possessing different alleles at two mating type loci for successful host infection. The a locus encodes pheromones and receptors, while the b locus encodes subunits of a heterodimeric transcription factor that regulates expression of virulence genes. Mating is triggered by environmental signals, including nutrient deprivation. My goal was to determine the fate of nitrogen starved haploid cells without a compatible mating partner. On solid low ammonium media, wild-type U. maydis filaments. I examined …

Former Students Discuss Middle School Science Journalism : A Qualitative Study Of Personally Meaningful Disciplinary Writing., Marsha R. Buerger

Electronic Theses and Dissertations

This qualitative research study explores the experiences of six middle school students with science news writing (SciJourn) after they have transitioned to high school. The qualitative method of case study was used with the data analyzed through the method of constructivist grounded theory. SciJourn is a disciplinary approach to science literacy that allows students to choose and research their own topic, interact with experts in the field, construct their own knowledge, and have the opportunity to publish in an on-line science newspaper (Scijourner.org). Theoretically, this research draws on science as social practice where literacy learning is cognitively complex, is situated …

Characterizing Reactive Glutamines In Fibrinogen And Elucidating Factor Xiii Substrate Specificity., Kelly Njine Mouapi

Electronic Theses and Dissertations

Fibrinogen is the most abundant protein involved in blood coagulation and has been associated with many pathological implications in cardiovascular disease. At the final stages of blood clot formation, the transglutaminase Factor XIIIa introduces γ-glutamyl-ε-lysinyl covalent bonds between reactive glutamines and lysines in fibrin, which results in a tighter clot network that is resistant to fibrinolysis. Factor XIIIa crosslinks specific reactive glutamines on fibrinogen, selecting more reactive glutamines in the αC region of fibrinogen than any other chain. Although crosslinking pairs in the αC region have been identified, little is known about the extent of crosslinking and the role played …

Likelihood-Based Methods For Analysis Of Copy Number Variation Using Next Generation Sequencing Data., Udika Iroshini Bandara

Electronic Theses and Dissertations

A Copy Number Variation (CNV) detection problem is considered using Circular Binary Segmentation (CBS) procedures, including newly developed procedures based on likelihood ratio tests with the parametric bootstrap for models based on discrete distributions for count data (Poisson and negative binomial) and a widely-used DNAcopy package. Results from the literature concerning maximum likelihood estimation for the negative binomial distribution are reviewed. The Newton-Raphson method is used to find the root of the derivative of the profile log likelihood function when applicable, and it is proven that this method converges to the true Maximum Likeihood Estimate (MLE), if the starting point …

Magnetic Field-Induced Intramolecular Cyclization As A Trigger For Nanoparticle-Based Delivery Systems., Sara Katherine Biladeau

Electronic Theses and Dissertations

Magnetic nanoparticles (MNPs) are used in a variety of applications, including as agents for magnetic resonance imaging, generation of local hyperthermia, and as platforms for drug delivery. Iron-based MNPs are often coated with a shell, such as silica or gold, to increase biocompatibility for drug delivery applications. Many MNPs used for cancer therapy rely on either an internal trigger, such as a difference in pH, or an external trigger, such as light or an alternating magnetic field (AMF), to cause release of a payload, typically a chemotherapeutic drug. Internal triggers are appealing because drug release can be targeted to a …

Regularized Solutions For Terminal Problems Of Parabolic Equations., Sujeewa Indika Hapuarachchi

Electronic Theses and Dissertations

The heat equation with a terminal condition problem is not well-posed in the sense of Hadamard so regularization is needed. In general, partial differential equations (PDE) with terminal conditions are those in which the solution depends uniquely but not continuously on the given condition. In this dissertation, we explore how to find an approximation problem for a nonlinear heat equation which is well-posed. By using a small parameter, we construct an approximation problem and use a modified quasi-boundary value method to regularize a time dependent thermal conductivity heat equation and a quasi-boundary value method to regularize a space dependent thermal …

Mechanism Investigation Of Pseudouridine Synthases Trub And Rlua With Rna Containing 5-Fluorouridine And 4-Thiouridine., Uyen T. Duong

Electronic Theses and Dissertations

Pseudouridine synthases (Ψ synthases) are the enzymes that catalyze the isomerization of uridine (U) to pseudouridine (Ψ), which is the most prevalent post-transcriptional modification of RNA. The Ψ synthases fall into six different families that share no significant global sequence similarity; however, they all involve a conserved aspartic acid residue which is absolutely essential for activity. Tyrosine is a conserved residue in the active site in five of the six families of Ψ synthases (phenylalanine in the TruD family) and was hypothesized as the general base for the isomerization reaction. To confirm the function of Tyr-96, Y96F RluA was assayed …