Digital Commons Network^™

Synthesis Of 2,4,6-Substituted Pyrrolo[2,3-D]Pyrimidines As Potential Anticancer Agents, Si Yang

Electronic Theses and Dissertations

This thesis mainly focuses on the introduction of the background and work have been done in the areas of antifolates development, such as folate function, its three uptake mechanisms inside human cells, antifolates’ role in chemotherapy, et. al. In addition, the Structure-Activity-Relationship design rationale for the series of antifolates will also be discussed. Nevertheless, the details of synthesizing these pyrrolo[2,3-d]pyrimidines as potential antifolates have been described, including chemistry reviews on the pyrrolo[2,3-d]pyrimidine scaffold, and the challenges encountered and the solutions how to solve or improve in order to achieve better yield.

Pcb-Associated Steatohepatitis And The Role Of Nuclear Receptors., Heather Brooke Clair

Electronic Theses and Dissertations

Metabolic diseases, including fatty liver disease, hyperglycemia, and obesity, result when body systems responsible for managing allostasis (dynamic homeostasis across systems) are pressured beyond their collective compensatory reserve. Nutritional excess contributes to this state, the capacity of which is limited by genetic variation, and failure of one system will gradually lead to pathological overload in the others. Agents which act directly on the communication machinery linking these connected systems can also change the point at which allostatic load becomes allostatic overload. Environmental exposure to polychlorinated biphenyls (PCBs), a class of persistent organic pollutant, is associated with a specific form of …

Role Of Human Arylamine N-Acetyltransferase 1 In Tumorigenesis And Cancer Biology., Marcus William Stepp

Electronic Theses and Dissertations

Human arylamine N-acetyltransferase 1 (NAT1) is a well-known phase II metabolic enzyme that has been associated with carcinogenesis. Its role in the biotransformation of aromatic and heterocyclic amine carcinogens has been investigated for many years, but more recent investigations focus on a possible endogenous role of human NAT1 in cancer initiation and progression. To further explore and understand possible endogenous roles for NAT1, we conducted in vitro enzymatic reactions, in vivo studies using homozygous high (rapid) or low (slow) NAT2 activity congenic F344.WKY rats, and genetically modified human breast cancer cell lines. Human NAT1 and rodent NAT2 hydrolyzed acetyl-coenzyme …

This Is Just A Phase : The Impact Of Population Structure On Haplotype Phasing And Linkage Disequilibrium Measures At Functional Genetic Sites., Roxanne Kaaren Leiter

Electronic Theses and Dissertations

The block-like structure of the human genome has been the subject of many scientific papers and is of practical significance in large-scale genome-wide association studies. How stringent haplotype block boundaries are within and between populations has been the subject of ongoing debate within human population genetics. This thesis will contribute to the description of universal and population-specific haplotype blocks at functional sites, namely across the IL-10 gene family (including IL-10, IL-19, IL-20 and IL-24), which is involved in a number of immune system processes, and MAPKAP-K2, an adjacent and functionally significant kinase gene. Beyond the description of blocks across these …

Chemoselective Reagents For Derivatization Of Trace--Level Volatile Carbonyl Compounds Using A Microreactor Approach., Mumiye A. Ogunwale

Electronic Theses and Dissertations

Detection and analysis of trace level volatile aldehydes and ketones has become a significant research frontier because of the applicability for environmental monitoring and assessment, noninvasive diseases diagnosis, and in food safety assessment for the US Food and Drug Administration. The number of derivatization reagents for detection of aldehydes and ketones has increased considerably over the last decade. However, the majority of these derivatization reagents are not efficient in derivatizing unsaturated carbonyl compounds due to the presence of electron withdrawing groups adjacent to the reactive functional moieties making them insufficiently nucleophilic. The analysis of trace-level carbonyl compounds challenges existing analytical …

The Role Of Kinases And Phosphatases In The Pathobiology Of Porphyromonas Gingivalis., Sarah Whitmore

Electronic Theses and Dissertations

Periodontal diseases result from the interplay between the dysregulation of the host inflammatory response and the actions of a dysbiotic bacterial community. These chronic inflammatory conditions affect persons on a worldwide scale. P. gingivalis is strongly implicated as a key periodontal pathogen in the more severe manifestations of periodontal diseases such as periodontitis. A core element of P. gingivalis pathogenicity is its dysregulation of innate immunity, including suppression of IL-8 production by gingival epithelial cells. The NF-κB family of related transcription factors plays a central role in regulating many important aspects of innate immune responses. NF-κB RelA/p65 homodimers regulate transcription …

Since The Time Of Eve : La Leche League And Communities Of Mothers Throughout History., Joanna Paxton Federico

Electronic Theses and Dissertations

La Leche League International (LLL) is the oldest and largest breastfeeding support group in the world. This thesis examines how, beginning in 1956, seven Catholic housewives from suburban Chicago built up the institutional knowledge to sustain a cohesive global network of breastfeeding mothers. It also explores how LLL managed this knowledge over time in response to developments in scholarship and changing social conditions. Based on a narrative analysis of LLL publications, this thesis argues that the League’s founders drew selectively from existing bodies of knowledge and from their own cultural perspectives to establish a sense of community among breastfeeding women. …

Deficiency Of Rdh10 Causes Cleft Secondary Palate In Mouse., Swetha Raja

Electronic Theses and Dissertations

The focus of this project is to understand the role of Retinoic Acid (RA) produced by RDH10-mediated metabolism of Vitamin A in palate development. For 80 years now, we have known that dietary restriction of RA/Vitamin A can cause cleft palate but we have failed to understand how RA deficiency causes cleft palate. Our gap in knowledge about how RA deficiency causes cleft palate is mainly owing to the fact that we lack a model system to study such defects. The aim of this thesis research project was to determine if conditional inactivation of the Vitamin A metabolic gene Rdh10 …

Type Ix Secretion System: Characterization Of An Effector Protein And An Insight Into The Role Of C-Terminal Domain Dimeration In Outer Membrane Translocation., Lahari Koneru

Electronic Theses and Dissertations

Porphyromonas gingivalis and Tannerella forsythia are two of the primary pathogens that are associated in the etiology and progression of chronic periodontitis. In T. forsythia, KLIKK proteases are the recently identified group of proteolytic enzymes that are secreted through Type IX secretion system (T9SS). Among, these KLIKK proteases a synergistic relationship was observed between karilysin and mirolysin in invading the host complement system for the survival of the bacteria. Since, karilysin has been already characterized, in this study we propose to study about mirolysin through structural, biochemical and biological characterization. The obtained results from the experiments has shown the …

The Feelings We Harbor - The Role Of Collage In Arousing Powerful Emotions In An Esl Classroom., Irina V. Mcgrath

Electronic Theses and Dissertations

Traditional literacy education in the U.S. relies exclusively on language-based models. This can be particularly challenging for English language learners, who often find themselves bound by the constraints of the English language and limited in their expressions of thought and emotion. Current research on English language teaching, largely targeted towards native English-speaking students, suggests that incorporating various alternate modes of communication and representation improves literacy learning. Studies that address English language learning for non-native speakers are sparse. The purpose of this arts-based, qualitative, descriptive study is to fill this gap in the literature. Using collage making as a communicative mode, …

Sample Size Calculations And Normalization Methods For Rna-Seq Data., Xiaohong Li

Electronic Theses and Dissertations

High-throughput RNA sequencing (RNA-seq) has become the preferred choice for transcriptomics and gene expression studies. With the rapid growth of RNA-seq applications, sample size calculation methods for RNA-seq experiment design and data normalization methods for DEG analysis are important issues to be explored and discussed. The underlying theme of this dissertation is to develop novel sample size calculation methods in RNA-seq experiment design using test statistics. I have also proposed two novel normalization methods for analysis of RNA-seq data. In chapter one, I present the test statistical methods including Wald’s test, log-transformed Wald’s test and likelihood ratio test statistics for …

Self-Management As A Mediator Of The Relationship Between Social Support And Health Outcomes Of African American Adults With Type 2 Diabetes., Tariq N. Al-Dwaikat

Electronic Theses and Dissertations

Type 2 diabetes (T2D) is often associated with serious complications. African American adults have higher rates of diabetes-related complications than other ethnicities. Diabetes self-management reduces the risk of developing biological and psychological symptoms. Social support promotes positive behavior change and self-management that leads to improved biobehavioral and psychosocial outcomes. Few studies explored the relationship between social support dimensions and self-management behaviors, diabetes biomarkers, and psychosocial outcomes of African American adults with T2D. The purpose of this dissertation was to examine the relationships of various dimensions of social support with self-management behaviors and diabetes biomarkers and psychosocial outcomes of African American …

A Spiritual Framework Of Organic Oneness: The Eco-Theological Shift Towards A Collective Ecological Consciousness, Kjirsten Francisco José Durand

Electronic Theses and Dissertations

Earth’s environment has been undergoing unprecedented rates of degradation during the Anthropocene paradigm. Current projections for the near future show climate change producing grim outcomes for most habitable parts of the world. This thesis defends the argument that in order to adequately address the state of the environment, humanity must experience a shift in collective consciousness away from the current philosophical paradigm, and instead adopt a paradigm that enables a common mindset regarding the place of humans within the natural environment. Various forms of spiritual ecology are explored: deep ecology, biblical eco-theology, and eco-feminist theology. These positions are explored in …

Evaluating Ligand Assisted Activation Of Small Molecules Through Proton Reduction And Alcohol Oxidation., Rahul Jain

Electronic Theses and Dissertations

Hydrogen is a potential alternate to the carbon fuels to meet the future demands for energy in an eco-friendly manner. Hydrogen produced by the electrolysis of water is stored in fuel cells which on demand can be converted into electrical energy upon the reaction with oxygen. In the quest for economically viable electrocatalysts for hydrogen evolution, the work in this dissertation describes evaluation of nickel based catalysts coordinated to redox active ligands, dialkyl/diarylphosphine benzenethiol and bis(thiosemicarbazone) for their ability to generate hydrogen with external acids as a proton source. A nickel complex with the benzenethiol ligand framework follows a traditional …

"It Teaches Them To Have A Conversation In The Real World" : An Analysis Of How Identity Performances Shape Classroom Discussions., Ashley Lauren Shelton Arnold

Electronic Theses and Dissertations

Although discussion has long played a role in classroom learning, recent focus on moving discussion away from the evaluative and toward the dialogic is key to understanding how discussion can be driven by multiple, shared understandings. Adopting a sociocultural perspective, this dissertation explores the ways in which identity performances shape one teacher and his students’ participation in classroom discussions. The theoretical framework guiding this study draws on the relationship between discourse and identities construction, with further attention given to improvisations of identities, figured worlds, and the ways in which power shapes discourse. This dissertation argues that performed identities shape the …

Age-Related Host Factors Regulate Lung Cancer Progression., John Caleb Greenwell

Electronic Theses and Dissertations

Cancer is largely a disease of the elderly. In the United States alone, 87% of all cancers in 2017 will be diagnosed in individuals aged 50 years or older. Among them, lung cancer is the deadliest, accounting for 1 in 4 cancer deaths. The 5-year survival rate is below 20%, a number which has not changed substantially over the past several decades. This underscores a desperate need for new strategies in prevention, early detection and treatment of this deadly disease. While tobacco use is unquestionably the number one risk factor for the development of lung cancer, the majority of diagnoses …

A Novel Approach To Assess Minimally Invasive Surgical Device Failure Utilizing Adverse Event Outcome Severity And Design Complexity., Marie K. Riggs

Electronic Theses and Dissertations

Medical device failure and misuse have the potential to cause serious injury and death. Given the intricate nature of the instruments utilized specifically in minimally invasive surgery (MIS), users and manufacturers of surgical devices share a responsibility in preventing user error and device failure. A novel approach was presented for the evaluation of minimally invasive device failures, which involved assessing the severity of adverse event outcomes associated with the failures modes and investigating aspects of the devices’ design that may contribute to failure. The goals of this research were to 1) characterize the design attributes, failure modes, and adverse events …

Nonspreading Solutions In Integro-Difference Models With Allee And Overcompensation Effects., Garrett Luther Otto

Electronic Theses and Dissertations

Previous work in Integro-Difference models have generally considered Allee effects and over-compensation separately, and have either focused on bounded domain problems or asymptotic spreading results. Some recent results by Sullivan et al. (2017 PNAS 114(19), 5053-5058) combining Allee and over-compensation in an Integro-Difference framework have shown chaotic fluctuating spreading speeds. In this thesis, using a tractable parameterized growth function, we analytically demonstrate that when Allee and over-compensation are present solutions which persist but essentially remain in a compact domain exist. We investigate the stability of these solutions numerically. We also numerically demonstrate the existence of such solutions for more general …

Graph Analytics Methods In Feature Engineering, Theophilus Siameh

Electronic Theses and Dissertations

High-dimensional data sets can be difficult to visualize and analyze, while data in low-dimensional space tend to be more accessible. In order to aid visualization of the underlying structure of a dataset, the dimension of the dataset is reduced. The simplest approach to accomplish this task of dimensionality reduction is by a random projection of the data. Even though this approach allows some degree of visualization of the underlying structure, it is possible to lose more interesting underlying structure within the data. In order to address this concern, various supervised and unsupervised linear dimensionality reduction algorithms have been designed, such …

Language Proficiency Attainment And Mobility Among Ell Students, Elizabeth Ayers Neill

Electronic Theses and Dissertations

The impact student mobility has on academic achievement has been researched in the United States since the early 20^th century (Goebel, 1978). Mobility for students is a risk factor often compounded by poverty, ELL students are at a higher risk of lower achievement. Educators face challenges in tracking records, monitoring, remediating, gap closing, and assisting students in transition periods. The data collected in this quantitative study was analyzed to determine the impact mobility has on English language learners and their attainment of a second language. This quantitative study examined the relationship between non-mobile and highly mobile ELL students in …

Preschool Enrollment And Elementary School Achievement In An East Tennessee School District, Derek Voiles

Electronic Theses and Dissertations

The purpose of this study was to determine if a significant difference in achievement scores exists among students based on whether or not they attended preschool as measured by standardized achievement reading-language arts and math scores on the Tennessee Comprehensive Achievement Program assessment for fourth grade students in a single East Tennessee school district. The variables of grade level and preschool attendance were considered. The population consisted of fourth grade students during the 2014-2015 school year. Data were gathered from the Tennessee Comprehensive Assessment Program achievement test scores obtained from the 2014-2015 school year and from the school district’s preschool …

Identities And Persistence Of Family Farm Operators, Parker T. Arnold

Electronic Theses and Dissertations

This study focuses on the identities of family farm operators and the challenges to maintaining viable farm operations in today’s agricultural economy. Employing a grounded qualitative approach, the author conducted 18 in-depth interviews with principal farm operators from Iowa and Tennessee. Using the insights of farmers from geographically different agricultural regions, this study notes how preserving family histories, socialization processes, and farming as a moral career inform operators’ understandings of themselves and the work they do. The analysis also focuses on how family farm operators contend with a globalized agricultural economy and the moral and ethical concerns of managing a …

Prescription Drug Abuse And Provider-Patient Communication: A Qualitative Analysis Of The Perspectives Of Prescribers And Patients, Stephanie M. Mathis

Electronic Theses and Dissertations

Prescription drug abuse is a public health problem of epidemic proportions in the United States. Provider-patient communication underpins many initiatives aimed at preventing and reducing the public health burden of prescription drug abuse. The characteristics of and factors contributing to this interpersonal process, however, have not been fully explored.

The purpose of this research was to examine: 1) the overall problem of prescription drug abuse and provider-patient communication about prescription drug abuse from the patient perspective; and 2) provider-patient communication about prescription drug abuse from the prescriber perspective. In 2014-2015, semi-structured interviews were conducted with 20 patients from primary care …

Social-Ecological Factors Affecting Patient Shield Use Among Radiologic And Computed Tomography Technologists, Megan Housenick-Lee

Electronic Theses and Dissertations

Medical radiation is estimated to contribute to over 200,000 deaths annually. Recent increases in the use of radiation-producing medical imaging examinations have led to increasing cumulative radiation dose to the general public. Multiple measures have been taken to address this alarming trend, including physician education, technologist education on dose reduction, and equipment-facilitated dose reduction techniques. Shield use can reduce the primary beam by up to 95%. Medical imaging technologists are the primary individuals responsible for applying shielding during an examination. Currently, literature shows that technologists are not shielding individuals as often as they should.

After pilot testing, medical imaging technologists …

Only The Earth Remains: Exploring The Machine In Selected Lyric Poetry Of Robinson Jeffers, Mark Hutton

Electronic Theses and Dissertations

In The Machine in the Garden: Technology and the Pastoral Idea in America, Leo Marx “evaluates the uses of the pastoral ideal in the interpretation of American experience” (Marx 4). While Marx explores ways that pastoralism has been impacted by factors such as industrialism, it is the purpose of this project to explore Marx’s assertion regarding the presence of the figurative and literal machine within the poetry of Robinson Jeffers.

Jeffers’ poetry is generally located within the landscapes of California. His lyric poetry has a distinct connection to the land and is driven by inhumanism, which works to shift …

Access To Healthy Foods: A Descriptive Analysis Of Farmers’ Markets, Food Deserts & Usda Food Assistance Programs In Tennessee Census Tracts, Twanda D. Wadlington

Electronic Theses and Dissertations

Food deserts are a growing problem in the United States, and occur in areas of low-income where people have limited access to healthy foods. In response, the presence of farmers’ markets has grown exponentially, and improved healthy food access. Additionally, the USDA has strived to connect families to healthy foods through food assistance programs such as the Supplemental Nutrition Assistance Program (SNAP), the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC), and the Senior Farmers’ Market Nutrition Program (SFMNP). This study investigated the relationship between farmers’ markets, their acceptance of food assistance benefits, and their locations within Tennessee …

Native And Community College Transfer Students In Biological Sciences At A Four-Year Institution: A Comparative Study, Nathanial Weber

Electronic Theses and Dissertations

The purpose of this study was to investigate differences between native and community college transfer students and identify factors that predicted upper-level biology course grade-point average and final overall grade-point average at a four-year institution in biological sciences. The results of this study indicated four-year institution persistence was not significantly related to gender, high school grade-point average, or ACT composite score. Persistence was significantly related to transfer status; whether the student was a native or community college transfer student with native students persisting at a higher rate at the four-year institution than community college transfer students. Furthermore, ACT composite score, …

Online Learning In Biology: An Investigation Into Designing Online Learning Resources, William Ford

Electronic Theses and Dissertations

As technology continues to advance, many instructors are incorporating online activities into their courses. While online learning has several benefits, there is still debate on how instructors can best develop and utilize these resources in their classroom. This study is split into two smaller projects that both aim to provide further insights on how to develop online activities that target undergraduate biology students. The first project revealed that elaborative feedback in a phylogenetic activity was more useful for students who had some exposure to phylogenetics prior to completing the activity. The results of the second project revealed that the appearance …

Design And Validation Of A Scale For Preschoolers: Measuring Nutrition Knowledge, Beliefs, And Behaviors, Michelle E. Johnson

Electronic Theses and Dissertations

The literature indicates a lack of validated scales to measure nutrition knowledge, attitudes and behaviors in preschool-aged children. Reliable and valid assessment tools are critical in the development of nutritional interventions for young children; careful design, including psychometric evaluation of reliability and validity, is the best approach to scale development. Best practice points to an efficient, yet comprehensive look at the constructs of knowledge, attitudes, and behaviors. This multi-phase study included 298 preschool children ages 2-6 years (151 girls, 147 boys; mean age 3.936). A 45-item scale was created, with 4 subscales to measure the nutrition knowledge, attitudes, and behaviors …

The Effects Of A Supermarket Tour On Improvement Of Nutrition Knowledge And Eating Behavior, Elizabeth Hall

Electronic Theses and Dissertations

The purpose of this study was to determine if participation in a supermarket tour improves nutrition knowledge and eating behavior in adult participants. Participants were recruited in communities surrounding Food City stores, a local supermarket. Prior to completing a standardized tour, participants completed a survey to assess nutrition knowledge and eating behavior. This survey was given to participants again three months later. A program evaluation was given one time at the end of the tour. Data analysis revealed no significant findings, other than the behavior-based question: “How many meals or snacks on most days included vegetables”. Vegetable consumption appeared to …