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Revisiting Neutrophil Responses To Toll-Like Receptor 4 : Influence Of Ligand Structures And Cellular Environments., Shuvasree Sengupta

Electronic Theses and Dissertations

Neutrophils respond to bacterial LPS through Toll-like receptor 4 (TLR4), which activates or potentiates immune defensive functions and prolongs cell survival. Activation of TLR4 signaling in neutrophils is beneficial for effective clearance of LPS-bearing Gram-negative bacteria, but may also drive aberrant inflammation if not stringently regulated. The regulatory processes by which neutrophil functions are calibrated to respond appropriately to different LPS-bearing bacteria are incompletely understood. Described here are investigations that reveal an unexpected sensitivity of TLR4 in neutrophils to small changes in LPS structure typical of various Gram-negative bacteria, including those that are dangerously virulent (Escherichia coli and Salmonella …

Algorithms For Automated Assignment Of Solution-State And Solid-State Protein Nmr Spectra., Andrey Smelter

Electronic Theses and Dissertations

Protein nuclear magnetic resonance spectroscopy (Protein NMR) is an invaluable analytical technique for studying protein structure, function, and dynamics. There are two major types of NMR spectroscopy that are used for investigation of protein structure – solution-state and solid-state NMR. Solution-based NMR spectroscopy is typically applied to proteins of small and medium size that are soluble in water. Solid-state NMR spectroscopy is amenable for proteins that are insoluble in water. In the vast majority NMR-based protein studies, the first step after experiment optimization is the assignment of protein resonances via the association of chemical shift values to specific atoms in …

Articulating The New Normal(S) : Mental Disability, Medical Discourse, And Rhetorical Action., Andrew Wesley Holladay

Electronic Theses and Dissertations

“Articulating the New Normal(s): Mental Disability, Medical Discourse, and Rhetorical Action” studies the writing of people diagnosed with autism and post- traumatic stress disorder within online discussion boards related to mental health and outlines their unique rhetorical strategies for interacting with biomedical ideologies of psychiatry and activist discourses. The opening chapter situates this dissertation in relation to previous scholarship in Rhetoric, Disability Studies, and other fields. I also provide a summary of the set of mixed methods I use to gather and analyze my data, including rhetorical analysis, corpus analysis, and qualitative interviews. In Chapter 2, “Medical Terminology and Discourse …

Cumulative Trauma, Emotion Reactivity And Salivary Cytokine Response Following Acute Stress Among Healthy Women., Yvette Z. Szabo

Electronic Theses and Dissertations

This study furthers understanding of how trauma exposure may be connected to the development and maintenance of poor health by focusing on cytokines, tightly regulated proteins of the immune system. Cytokine responses to acute stress have been associated with the onset of poorer mental health in physically healthy women. The present study examined how two factors recently associated with cytokine reactivity –cumulative trauma and emotion reactivity– are associated with salivary cytokine reactivity among healthy women. Seventy-one women, screened to be physically and mentally healthy, completed a laboratory acute stress paradigm and self-report measures of state emotion and trauma exposure. Participants …

Advances In Tumor-Targeted Therapy Using Nanomedicine., Divya Karukonda

Electronic Theses and Dissertations

Despite continuous improvement and significant progress made in diagnostic and therapeutic approaches for cancer, it is still the leading cause of death worldwide. Although conventional chemotherapy has made significant advances in improving patient survival the indiscriminate destruction of normal cells leads to severe side effects and poor clinical outcomes. Thus, there is a need for effective delivery of drugs to the tumor site avoiding normal tissues to reduce toxicity in the rest of the body. For this reason, a novel multidisciplinary field called Nanotechnology has evolved in recent years and advances in this field have contributed to the development of …

Prevention And Treatment Of Familial Adenomatous Polyposis And Colorectal Cancer By Bilberry-Derived Anthocyanidins., Ashley M. Mudd

Electronic Theses and Dissertations

Colorectal cancer (CRC) is the third leading cause of cancer-related deaths within the United States. Familial adenomatous polyposis (FAP) is an inherited disorder which if left untreated will develop into colon cancer. The family of plant-derived compounds, anthocyanins, show significant therapeutic potential against a variety of diseases, however, they are limited by their instability and poor bioavailability. The goal of my Master’s research project was to determine whether anthocyanidins (non-glycosylated anthocyanins) are more effective than the native anthocyanins, and whether exosomal formulation of anthocyanidins (ExoAnthos) can enhance therapeutic potency compared with free Anthos against both FAP and CRC. The antiproliferative …

American Muslim Men And Stigma : The Use Of Religious Communities As Mechanisms For Stigma Management., Jack R. Allen Iii

Electronic Theses and Dissertations

The experiences of American Muslim men deserve further study because they are relevant to current civil rights discussions, especially in a social-political climate that is growing increasingly hostile toward the Islamic world. Despite acknowledging the significant impact of gender, relatively little research has focused exclusively on masculine Muslim experiences of stigmatization. This research addresses the experiences of religiously practicing Muslim men and addresses the following questions: How do Muslim men experience stigma in their communities? What experience do these men have with religious institutions? And finally, how do these experiences with religious institutions inform how these individuals manage stigma? Using …

Bayesian Approach On Short Time-Course Data Of Protein Phosphorylation, Casual Inference For Ordinal Outcome And Causal Analysis Of Dietary And Physical Activity In T2dm Using Nhanes Data., You Wu

Electronic Theses and Dissertations

This dissertation contains three different projects in proteomics and causal inferences. In the first project, I apply a Bayesian hierarchical model to assess the stability of phosphorylated proteins under short-time cold ischemia. This study provides inference on the stability of these phosphorylated proteins, which is valuable when using these proteins as biomarkers for a disease. in the second project, I perform a comparative study of different confounding-adjusted to estimate the treatment effect when the outcome variable is ordinal using observational data. The adjusted U-statistics method is compared with other methods such as ordinal logistic regression, propensity score based stratification and …

Preparing For Racial Discrimination : The Role Of Cognition And Emotion In The Proactive Coping Process Of African American College Students., Ryan Christopher Tyler Delapp

Electronic Theses and Dissertations

Traditionally, conceptual models of racial discrimination have characterized the reactive experiences of African Americans, particularly identifying how African Americans cognitively, emotionally, and behaviorally respond to racial stress. The current study extends beyond the reactive coping experience and identifies nuances in the anticipatory and preparatory coping processes associated with racial discrimination. Methods: 62 African American college students participated in a stress induction experiment that prompted anticipatory judgments of discrimination. The full sample completed quantitative self-report questionnaires about their anticipatory thoughts (SAM; Peacock & Wong, 1990; Roesch & Rowley, 2005), state-based affect (PANAS-X; Watson & Clark, 1994), and proactive coping behaviors (PPCB; …

Characterization Of Porphyromonas Gingivalis Mfa1 Fimbriae., Jae Yong Lee

Electronic Theses and Dissertations

Porphyromonas gingivalis, an obligate anaerobic bacterium associated with chronic periodontitis, utilizes various virulence factors to achieve pathogenicity, one of which is the Mfa1 fimbriae. As a surface structure comprising Mfa1 major subunit along with accessory fimbrial proteins Mfa2-5, the Mfa1 fimbriae has been shown to mediate the adherence of P. gingivalis to antecedent bacterial colonizers of the oral cavity to cause increased virulence. However, the spatial relationships amongst the individual subunits and their assembly mechanism have remained unclear. Through immuno-electron microscopy, Mfa1-4 were localized on the surface of P. gingivalis with Mfa1 localizing throughout the fimbriae and Mfa2 in …

Transient Absorption Studies Of Cdse Nanocluster Passivated With Phenyldithiocarbamate Ligands., Yizhou Xie

Electronic Theses and Dissertations

Semiconductor nanocluster (SCNC) research is a rapidly growing field driven by the promising fact that properties of such materials can be tailored by modifying their size, shape, and structure. Combination of nanocluster and organic ligands provides even wider possibilities of design and development of effective and task-specific nanostructures. Understanding of and, eventually, control of energetics, interfacial interaction, and photoinduced processes in such highly heterogeneous structures is critical to invention of novel materials including those in photovoltaic devices. In the newly established Ultrafast Laser Facility (ULF), transient absorption pump-probe spectroscopy (TAPPS) has been employed to investigate the electron transfer (ET) and …

Engineering Applications Of Organic Surfactant Modified Bentonite In Sorptive Soil Barriers., Sadra Javadi

Electronic Theses and Dissertations

Earthen barriers such as CCLs and GCLs have been employed in geotechnical practices to provide a low permeability hydraulic barrier since long time ago. These types of barriers exhibited satisfactory performance for many applications such as landfills. The performance of low permeability barriers is based on the swelling potential of their component, which is mostly Na-bentonite, in contact with polar fluids such as water. However, the acceptable range of conductivity cannot be achieved by traditional earthen barriers when they are permeated by non-polar fluids such as gasoline. This phenomenon occurs due to the incompatibility of earthen barrier constituent with non-polar …

Gene Expression Of Ammonium Transporters In Ustilago Maydis And Their Role In Pathogenicity And Virulence., Kirsten Richardson

Electronic Theses and Dissertations

The fungus, Ustilago maydis, a plant pathogen of Zea mays, is capable of a dimorphic switch, e.g., a transition between a yeast-like form and a filamentous form. This switch is activated when U. maydis cells are starved for their preferred nitrogen source - ammonium. U. maydis has two important genes that play a role in this switch, ump1 and ump2. Each gene is responsible for the uptake of ammonium and the latter is responsible for the filamentous response in the absence of ammonium. Ump2 deletion mutants are unable to filament in response to low ammonium while ump1 deletion …

Vertex Weighted Spectral Clustering, Mohammad Masum

Electronic Theses and Dissertations

Spectral clustering is often used to partition a data set into a specified number of clusters. Both the unweighted and the vertex-weighted approaches use eigenvectors of the Laplacian matrix of a graph. Our focus is on using vertex-weighted methods to refine clustering of observations. An eigenvector corresponding with the second smallest eigenvalue of the Laplacian matrix of a graph is called a Fiedler vector. Coefficients of a Fiedler vector are used to partition vertices of a given graph into two clusters. A vertex of a graph is classified as unassociated if the Fiedler coefficient of the vertex is close to …

Emotion Regulation And Eating: An Evaluation Of The Relationship Between Difficulties In Emotion Regulation And Eating Patterns In Obese Patients Seeking Bariatric Surgery, Brittany V. Williams

Electronic Theses and Dissertations

Obesity is a major health epidemic, impacting many people worldwide. Bariatric surgery is a common treatment for severe obesity and generally leads to improved overall health, remission of comorbid disease, and improved quality of life. Despite positive postsurgical results, many patients regain some to most of their weight following the procedure. Guidelines for presurgical psychological assessments have been developed to assist healthcare professionals in predicting outcomes for patients. Previous studies have focused on the impact of psychological illness on surgical outcomes, with mixed results. The current study aimed to assess the influence that difficulties in emotion regulation has on eating …

Cyberbullying Incidents Among African American Female Middle School Students, Yvette Pennington

Electronic Theses and Dissertations

Recent research has shown an increase in cyber bullying acts against middle and high school students. The National Center of Education Statistics (2010) reported that cyberbullying incidents increased 73% between the years of 2007 and 2009. In 2011, 75% of cyberbullying victims were adolescents (National Center of Education Statistics, 2013). Using data collected from the Pew Research and American Life Project, the study examined the prevalence of cyber bullying acts against African American female adolescents compared to Caucasian male and female adolescents and African American male adolescents. Additionally, the study reported the cyber bullying incident that occurred most frequently as …

Effects Of Acute Sepsis On Renal Structure And Sympathetic Innervation In Mice, Tuqa Alkhateeb

Electronic Theses and Dissertations

Sympathetic nerves are important for renal physiology and sepsis pathophysiology. A recent study showed sprouting of sympathetic nerves in spleen of septic mice. This study was done to test if renal sprouting of sympathetic nerves also happens and to investigate renal morphology in septic mice. Cecal ligation and puncture (CLP) was used to induce sepsis and kidneys were removed for evaluation. Bowman’s space was diminished with cortical bubble cells present suggestive of acute renal pathology, however, renal function was unchanged. Acute sepsis did not affect either renal sympathetic innervation or non-neuronal cholinergic cells. Mouse kidneys had more epinephrine (EPI) than …

Ecological Informatics: An Agent Based Model On Coexistence Dynamics, Shiva Thapa

Electronic Theses and Dissertations

The coexistence of species is probably one of the most interesting and complex phenomenon in nature. We constructed an agent based model to study the coexistence dynamics of prey - predator populations by varying productivity levels of producers in fragmented and connected habitats along with different levels of quality of predators. Our results indicated that productivity levels of producers in fragmented and connected habitats along with levels of predator quality are significantly responsible for overall predator - prey population size and survivorship. In the absence of predation, competition between identical prey populations is more probable in connected habitats than in …

Mirna Expression Changes In Arsenic-Induced Skin Cancer In Vitro And In Vivo., Laila Al-Eryani

Electronic Theses and Dissertations

Arsenic is a naturally prevalent metalloid. Chronic arsenic ingestion through drinking water causes skin cancer. Arsenic-induced cancer mechanisms are not well defined. Epigenetic changes, including microRNA expression changes, might be playing a role. This dissertation investigates the impact of miRNA expression changes in arsenic-induced skin cancer. MiRNA expression was measure and compared using 3 different techniques, RTq-PCR, hybridization arrays and RNA-sequencing. MiRNAs differential expression in skin lesions was phenotype- and stage-related. Immortalized human keratinocytes (HaCaT) were transformed by chronic low arsenite exposure serving as a model for arsenic-induced skin carcinogenesis. Early changes in miRNAs and target mRNAs contribute to arsenic-induced …

A Novel Method For Synthesis Of Hydroxytyrosol, Emmanuel Onobun

Electronic Theses and Dissertations

Hydroxytyrosol, 3,4-dihydroxyphenolethanol, a naturally occurring polyphenol most common in olive tree (Olea europaea), is one of the most effective member of the polyphenols family, because of its remarkable antioxidant activity, its ability to inhibit oxidation of low density lipids (LDL), and its protection against DNA oxidative damage. Hydroxytyrosol, which is widely used in cosmetics and food supplements industries, can be purchased as an olive oil extract that contains low concentration of hydroxytyrosol besides other polyphenols. The price and low natural abundance of hydroxytyrosol make alternative synthetic sources very attractive. In this research, a novel method for the synthesis of pure …

Matter Under Mind, John F. Lause

Electronic Theses and Dissertations

The artist discusses the work for his Masters of Fine Arts exhibition, Mind under Matter, held at the Tipton Gallery in downtown Johnson City, Tennessee. Exhibition dates are from March 27th through April 5th 2017. ‘Matter under Mind’ explores the balance of control and non-control within the art-making process. This technique creates an automatic dialogue resulting in abstraction guided by the subconscious. The title ‘Matter under Mind’ is a slight play on the phrase ‘mind over matter’ emphasizing how matter/material is manipulated by the mind through the making of artwork, and within the mind’s eye or imagination.

The video installation …

Evaluating A Stepped Care Protocol For Postpartum Depression In A Pediatric Primary Care Clinic, Sarah C. Smith

Electronic Theses and Dissertations

Postpartum depression (PPD) is a prevalent, complex illness impacting 10% to 20% of mothers and their families. Treatments for PPD, such as medication and psychotherapy, are effective at reducing the severity of symptoms and generally improving quality of life for new mothers and their families. Unfortunately, many mothers with PPD go unrecognized due to a lack of standardized screening methods. Further, mothers regularly encounter barriers to accessible, evidence-based follow-up care to treatment even when symptoms of PPD are detected. The use of a stepped care protocol, set in a pediatric primary care clinic, is one proposed strategy to address the …

The Tennessee Educator Acceleration Model And The Teacher Instructional Growth For Effectiveness And Results Model On Measures Of Teacher Effectiveness: A Comparative Study, Nichole R. Morris

Electronic Theses and Dissertations

The purpose of this quantitative study was to compare measures of teacher effectiveness between two different Tennessee teacher evaluation models. The two teacher evaluation models compared were the Tennessee Educator Acceleration Model (TEAM) and the Teacher Instructional Growth for Effectiveness and Results Model (TIGER). The measures of teacher effectiveness used were final observation scores and individual value-added growth (TVAAS) scores. The relationship between observation scores and growth scores were also compared for the two different evaluation models. There were four guiding research questions and corresponding null hypotheses. Data were provided from participating school systems. The sample for this study included …

Homework Practices: Teacher And Parent Perceptions Of Efficacy And Purpose, Christy E. Hoeke

Electronic Theses and Dissertations

The purpose of this study was to explore teacher and parent perceptions of homework effectiveness and homework purpose. Specifically, the researcher examined teacher and parent perceptions, perceptions of teachers in different grade level bands, and perceptions of teachers with different levels of experience. In this nonexperimental quantitative study, the researcher examined and analyzed the answers provided on an anonymous survey (Appendix A) provided electronically to educators and parents of students in five school systems in northeast Tennessee.

Parents had a higher perception of both homework effectiveness and homework purpose. Homework effectiveness was perceived as higher by the “other” group (not …

Opal Clark On Directing Stop Kiss An Exploration Of The Directorial Process, Opal Clark

Electronic Theses and Dissertations

The intent of this thesis and thesis project was to execute a successful run of the play, Stop Kiss by Diana Son while achieving the playwright’s intended message. Opal Clark directed this production of Stop Kiss at East Tennessee State University under special arrangement with Dramatists Play Service, Inc. It was performed to sold out audiences in ETSU Theatre and Dance Studio 205 October 2-7, 2017. The play explores experiences and hostility toward same sex couples, their relationships with one another and how one individual discovers sexuality. The discovery and actualization of the characters’ struggles were communicated in the play …

Role Of Tlrs, Hippo-Yap1 Signaling, And Micrornas In Cardiac Repair And Regeneration Of Damaged Myocardium During Ischemic Injury, Xiaohui Wang

Electronic Theses and Dissertations

Cardiovascular disease is a leading cause of death in the United States. Toll-like receptor (TLR)-mediated pathways have been demonstrated to play a role in myocardial ischemia/reperfusion (I/R) injury. We and others have shown that PI3K/Akt signaling is involved in regulating cellular survival and protecting the myocardium from I/R induced injury. In this dissertation, we provide compelling evidence that miR-125b serves to “fine tune” TLR mediated NF-kB responses by repressing TNF-a and TRAF6 expression. We constructed lentiviral expressing miR-125b, delivered it into the myocardium. The data showed that delivery of lentivirus expressing miR-125b significantly reduces myocardial infarct size and improves cardiac …

Enemy Exacerbation: Effects Of Predator Stress On Sulfate Lethality In Freshwater Amphipods (Gammarus Minus), Trevor Chapman

Electronic Theses and Dissertations

Predator cues can influence how aquatic organisms respond to anthropogenic contaminants. This study examined the effects of predator cues on behavior, metabolic rate, and sulfate (as Na₂SO₄) toxicity in amphipods (Gammarus minus). Predator cues included alarm cue (macerated conspecifics) and kairomone from mosquitofish (Gambusia affinis). Amphipods decreased activity and increased time in refuge when exposed to alarm cue, and increased time in refuge when exposed to kairomone. While median lethal concentrations (96-h LC₅₀) were not influenced by predator cues, analysis of dose response curves indicated that kairomone exposure increased amphipod …

Groundwater Flow Tracing In Carter Saltpeter Cave And Toll Branch, Carter County, Tn, Aaron G. Doyka

Electronic Theses and Dissertations

Groundwater tracing studies have shown a well-developed conduit system in the karst aquifer beneath the Buffalo Creek Watershed of Carter County, TN. Groundwater can quickly travel beneath topographic divides, transporting contaminants. Using GIS and field reconnaissance, two sites were selected for a two phase dye trace study. In May-June 2016 groundwater resurgences were located using activated carbon samplers, and in October-December 2016 flow velocities were measured using ISCO automatic water samplers. In study 1, a stream sinking in the back of Carter Saltpeter Cave was traced to its resurgence at Cave Springs Cave. In study 2, the dye mass centroid …

Vulnerability Assessment Of Groundwater To No3 Contamination Using Gis, Drastic Model And Geostatistical Analysis, Adela Beauty Adu Agyemang

Electronic Theses and Dissertations

The study employed Geographical Information System (GIS) technology to investigate the vulnerability of groundwater to NO₃ content in Buncombe County, North Carolina in two different approaches. In the first study, the spatial distribution of NO₃ contamination was analyzed in a GIS environment using Kriging Interpolation. Cokriging interpolation was used to establish how NO₃ relates to land cover types and depth to water table of wells in the county. The second study used DRASTIC model to assess the vulnerability of groundwater in Buncombe County to NO₃ contamination. To get an accurate vulnerability index, the DRASTIC parameters were …

Get Thee To A Nunnery: Unruly Women And Christianity In Medieval Europe, Sarah E. Wolfe

Electronic Theses and Dissertations

This thesis will argue that the Beowulf Manuscript, which includes the poem Judith, Saxo Grammaticus’s Gesta Danorum, and the Old-Norse-Icelandic Laxdæla saga highlight and examine the tension between the female pagan characters and their Christian authors. These texts also demonstrate that Queenship grew fragile after the spread of Christianity, and women’s power waned in the shift between pre-Christian and Christian Europe.