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Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Electronic Theses and Dissertations
Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …
Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti
Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti
Electronic Theses and Dissertations
This dissertation includes modeling of sporulation and germination behavior of Bacillus licheniformis strains during raw milk holding, tracking the survival of sporeformers and spores of high sporulating Bacillus strain during skim milk powder manufacturing. In addition, preliminary studies were done to standardize a rapid spore detection technique by using ratiometric fluorescence. To understand the role of strain variability during raw milk holding conditions, population dynamics of two strains of Bacillus licheniformis, ATCC 6634 and 14580, were modeled as a function of temperature (4.0 -12.0˚ C) and duration (0 - 72 h), using regression analysis. Based on initial spiking of vegetative …
Shrimp Production Environment And The Gut Microbiome: Effects Of Aquaculture Practices And Selective Breeding On The Gut Microbiome Of Pacific Whiteleg Shrimp, Litopenaeus Vannamei, Angela Landsman
Electronic Theses and Dissertations
Knowledge of the functional role of the gut microbiome in animal health and nutrition may provide solutions to shrimp aquaculture challenges, such as improving disease resistance and optimizing growth particularly with low cost feeds. Successful manipulation of bacteria found in the gut requires a deeper understanding of shrimp microbial communities and how their compositional structure is influenced by environmental conditions, and inherent host factors such as genetics. The initial research investigated the intestinal bacterial communities of the Pacific whiteleg shrimp (Litopenaeus vannamei) reared in pond systems compared to indoor aquaculture facilities as an exploration of the effects of aquaculture practices …
Novel Hybrid Analogs Of Estrone Origin Exhibits Cytotoxic Effects Against Egfr-Dependent Cancers, Felix Acheampong
Novel Hybrid Analogs Of Estrone Origin Exhibits Cytotoxic Effects Against Egfr-Dependent Cancers, Felix Acheampong
Electronic Theses and Dissertations
Cancer is second only to cardiovascular illnesses as the deadliest human disease globally. Currently, non-small cell lung cancer (NSCLC) and triple negative breast carcinoma (TNBC) are the most frequent types of cancer and have the highest mortalities. Epidermal growth factor receptor (EGFR), a central regulator of tumor progression, is frequently overexpressed in both cancers and is a key clinical target for therapeutic intervention. Natural products and their synthetic analogs have been utilized as EGFR tyrosine kinase inhibitors (TKIs) with potent antitumor effects. However, acquired resistance limits the long-term efficacy of these drugs. Estrone has been used as a scaffold in …
Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel
Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel
Electronic Theses and Dissertations
The human gastrointestinal tract is populated by a diverse and mutualistic microbial flora that has profound effect on human health. Identification and characterization of microbial-metabolites produced in response to human dietary intakes may provide insights into host-microbiota interactions to better understand the role of the microbiota in human health and diseases. However, much of this interaction is poorly understood as many metabolites remain unidentified or understudied at the present time. Starch is ubiquitously present in human diet and is a major energy source to all living cells. Resistant starch (RS) is the starch portion that is not absorbed in the …
Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh
Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh
Electronic Theses and Dissertations
Production of wheat is challenged by dynamic biotic and abiotic stresses. Genetic improvement via alien gene transfer is an effective approach to tackle such challenges. Alien gene transfer played an important role in the history of wheat crop improvement. Sea wheatgrass (SWG; Thinopyrum junceiforme, 2n = 28, genomes J1J1J2J2) is a wild relative of wheat. In our previous work, we have developed a complete amphiploid between cultivated emmer and SWG and shown that SWG is resistant to wheat streak mosaic virus, Fusarium head blight and wheat stem sawflies (due to the solid stem) and tolerant to waterlogging, salinity, heat, and …
Isolation And Identification Of Potential Bioinoculants Based On Phosphate Solubilizing And Plant Growth Promoting Benefits, Rachel Raths
Isolation And Identification Of Potential Bioinoculants Based On Phosphate Solubilizing And Plant Growth Promoting Benefits, Rachel Raths
Electronic Theses and Dissertations
Conservative models have shown that as populations rise, food production needs to double by 2050. Population increase and the green revolution have caused fertilizer inputs to increase since the 1960’s, increasing environmental issues and production costs. These intensive practices have led to degraded arable land and there has been an increase in urbanization meaning we need to make best use of the farming land that is available and ensure it is sustainable for future food production. Due to this, there has been a higher demand for research on more environmentally and economically friendly approaches to food production. Plant growth promoting …
Investigation Of Intergroup Bias In Two Neuromaturationally Distinct Age Cohorts: An Erp Study, Reuven M. Hanna
Investigation Of Intergroup Bias In Two Neuromaturationally Distinct Age Cohorts: An Erp Study, Reuven M. Hanna
Electronic Theses and Dissertations
Currently, sociological investigation of adolescent behavior focuses on the intersection of biography, history, and structure to explain adolescent risk-taking, reward-seeking, impulsivity, novelty-seeking and peer-salience. However, the preponderance of the evidence points away from social ecology and to a significant neuromaturational restructuring event between the 12th and 25th years of life as the root of adolescent behavioral tendencies. As a result, sociological social psychology can benefit from engaging in basic research using neuroscience methods. The present study expands the dual systems model of brain development to account for maturational changes in the social brain network as a way to explain social …
Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary
Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary
Electronic Theses and Dissertations
Sporeforming bacteria and their endospores have the ability to survive thermal treatments and proliferate in the milk and dairy products to cause spoilages. We applied the effect of hydrodynamic cavitation in controlling these bacteria and their spores at pilot scale level. Our aim was to investigate a technology that would improve the microbial quality of milk and dairy products, and is capable of being scaled up to be adapted at industrial level. Cavitation damages the cell walls of the bacteria and their endospores (releasing dipicolinic acid, responsible for endospore resistance to extreme conditions), thereby making them susceptible to thermal treatments …
Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes
Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes
Electronic Theses and Dissertations
Senecavirus A (SVA) is a positive sense single-strand RNA virus of the genus Senecavirus, family Picornaviridae. Senecavirus A is an emerging picornavirus of swine, which causes vesicular disease (VD) in pigs that is clinically indistinguishable from footand- mouth disease and other high consequence VDs of swine. There is evidence that SVA has been circulating in the US swine population since the late eighty’s, however, recently the virus emerged worldwide, and it has been associated with several VD outbreaks in pigs in many of the major swine producing countries. Despite the recent surge in the number of cases of …
Comparing Gut Microbiome And Virome In The Breast Milk- And Formula-Fed Late Preterm Infants, Zyi Wang
Comparing Gut Microbiome And Virome In The Breast Milk- And Formula-Fed Late Preterm Infants, Zyi Wang
Electronic Theses and Dissertations
The neonatal intestinal microbiome consists of all microorganisms in the gut. Although the microbiome is critical to human health and disease, its colonization remains incompletely understood, particularly in the preterm infant. We aimed to characterize the intestinal bacteria microbiome and virome in moderate-to-late preterm infants. We hypothesized that the bacteria microbiome and virome differs between breast milk and formula-fed infants. We collected stool samples from twenty infants born between 32 0/7 and 36 6/7 weeks gestation. Samples were collected after infants reached full volume enteral feedings. Ten infants were breast milk fed and ten received infant formula. DNA and RNA …
Design, Synthesis And Biological Screening Of Novel Cucs-Inspired Estrone Analogs Towards Treatment Of Pancreatic Adenocarcinoma, Khaled Alseud
Design, Synthesis And Biological Screening Of Novel Cucs-Inspired Estrone Analogs Towards Treatment Of Pancreatic Adenocarcinoma, Khaled Alseud
Electronic Theses and Dissertations
Pancreatic cancer is currently the fourth most deadly type of cancer globally. It is expected to become the second by 2030, due to its poor prognosis and its resistance to the current standard treatment, Gemcitabine. Moreover, the survival rate after treatment is low compared to other cancer types, which suggests an urgent need for new potent and safe treatment agents. Recently, the triterpenoid, cucurbitacin, has shown promising antiproliferative activity against human pancreatic cancer cells in a dose- and timedependent manner, and decreased pancreatic tumor size in combination with gemcitabine in vivo. However, the use of cucurbitacin in clinical studies has …
Greater Sage-Grouse Survival, Breeding Ecology, Resource Selection, And West Nile Virus Prevalence On The Eastern Fringe Of Their Range, Lindsey Anne Parsons
Greater Sage-Grouse Survival, Breeding Ecology, Resource Selection, And West Nile Virus Prevalence On The Eastern Fringe Of Their Range, Lindsey Anne Parsons
Electronic Theses and Dissertations
The greater sage-grouse (Centrocercus urophasianus) is a sagebrush (Artemisia spp.) obligate species that has experienced population declines over the past several decades. Sage-grouse are a species of conservation concern throughout the Intermountain West and are considered a species of greatest conservation need in South Dakota. Numerous studies have documented drivers of demographic performance at the core of their distribution; however, relatively few studies have examined sage-grouse inhabiting the eastern extent of their range, in South Dakota. We sought to examine sage-grouse space use in multiple seasons, estimate survival, and determine factors affecting nest success in South Dakota …
Preservice Agricultural Education Majors’ Perceptions Of Integrating Science Into Agriculture, Kasey Trocke
Preservice Agricultural Education Majors’ Perceptions Of Integrating Science Into Agriculture, Kasey Trocke
Electronic Theses and Dissertations
Agricultural science has seen a rise in secondary agricultural classrooms and many studies have been done with regards to integrating science into agriculture. Several recommendations have been to assist preservice agriculture education majors with science integration. Preservice agricultural education majors could influence the future of agricultural science due to their perceptions of integrating science into agriculture into the classroom. The purpose of the study was to assess preservice teachers’ perceptions of integrating science into agriculture curriculum based on the theory of planned behavior. An online survey was administered to the population of agricultural education majors at South Dakota State University. …
Efficacy Of Synthetic And Biopesticides On Bacteria Leaf Streak Management And Influence Of Cultivar And Environment On Epiphytic Bacteria Diversity On Wheat Seeds, Marilen Nampijja
Electronic Theses and Dissertations
Wheat (Triticum aestivum) is the most important staple food for over two billion people, or 36% of the world population. The United States ranks 4th in the world for wheat production. However, wheat production is faced by both biological and non-biological constraints. Among the biological constraints, diseases play a big role in limiting wheat production with estimated yield losses ranging from 20% to 40%. Bacterial leaf streak (BLS) of wheat caused by Xanthomonas translucens pv undulosa (Xtpvu) is one of the major bacterial diseases affecting the wheat production in most wheat growing regions in the USA. In recent years, it …
Design, Synthesis, And Evaluation Of Gunw-3 As A Brain Targeting Agent, Asim Najmi
Design, Synthesis, And Evaluation Of Gunw-3 As A Brain Targeting Agent, Asim Najmi
Electronic Theses and Dissertations
The blood brain barrier (BBB) is a barrier in the brain that separates the peripheral blood circulation system from the central nerve system (CNS). The barrier effectively protects the brain from xenobiotics. The BBB serves as a physical barrier through the tight junction of endothelial cells that were found to be 50-100 times tighter than that of normal endothelial cells. Different drug efflux pumps such as P-glycoproteins and multidrug-resistance proteins are found to be overdistributed on the BBB. These drug efflux pumps help pump xenobiotics out if they enter the cells and serv as an additional mechanism to prevent xenobiotics …
Characterizing Spatiotemporal Patterns Of White Mold In Soybean Across South Dakota Using Remote Sensing, Confiance L. Mfuka
Characterizing Spatiotemporal Patterns Of White Mold In Soybean Across South Dakota Using Remote Sensing, Confiance L. Mfuka
Electronic Theses and Dissertations
Soybean is among the most important crops, cultivated primarily for beans, which are used for food, feed, and biofuel. According to FAO, the United States was the biggest soybeans producer in 2016. The main soybean producing regions in the United States are the Corn Belt and the lower Mississippi Valley. Despite its importance, soybean production is reduced by several diseases, among which Sclerotinia stem rot, also known as white mold, a fungal disease that is caused by the fungus Sclerotinia sclerotiorum is among the top 10 soybean diseases. The disease may attack several plants and considerably reduce yield. According to …
Evaluation Of Feeding Alternative Feedstuffs Including Hydroponic Barley Sprouts And Carinata Meal To Dairy Cattle, Rhea D. Lawrence
Evaluation Of Feeding Alternative Feedstuffs Including Hydroponic Barley Sprouts And Carinata Meal To Dairy Cattle, Rhea D. Lawrence
Electronic Theses and Dissertations
The purpose of this dissertation research was to examine alternative feed ingredients not typically found in dairy cattle diets. In total, four studies were conducted to evaluate feedstuffs such as hydroponic barley sprouts and carinata meal and how they affect cattle performance. To determine how feeding hydroponic barley sprouts would affect growing dairy heifer and lactating cow performance two feeding studies were conducted. In the first study, inclusion of 14 % (DM basis) hydroponic barley sprouts (HYD) was evaluated in an ad libitum total mixed ration (TMR) compared to a control (CON) diet on dairy heifer performance during a 12 …
Effects Of Increasing Dietary Levels Of Carinata Meal On Feeding Behavior, Performance And Carcass Characteristics Of Beef Growing Steers, Alejandro C. Casella
Effects Of Increasing Dietary Levels Of Carinata Meal On Feeding Behavior, Performance And Carcass Characteristics Of Beef Growing Steers, Alejandro C. Casella
Electronic Theses and Dissertations
The objectives of the present study are to evaluate feeding behavior, animal performance and carcass characteristics of beef growing steers fed increasing amounts of carinata meal (CM). Due to the high protein content of the CM after the oil extraction for biofuel there is an opportunity to use it as a protein supplementation in beef cattle. Twenty-four steers blocked by weight were assigned to four corn-based diets (n = 6 animals per treatment). In the three treatment diets, carinata meal replaced high-moisture corn at 5, 10, or 15%. The diets were fed using the Insentec RIC system for 138 days. …
Nutritional Transporter Mediated Drug Delivery For Cancer, Siddharth S. Kesharwani
Nutritional Transporter Mediated Drug Delivery For Cancer, Siddharth S. Kesharwani
Electronic Theses and Dissertations
Recent advancements in nanotechnology have unfolded novel opportunities in medicine, especially in targeted therapeutics and imaging for cancer. However, the majority of the existing nanotechnologies for cancer suffer from shortcomings such as (i) rapid elimination from the systemic circulation before reaching the cancer tissue. (ii) poor tumor accumulation, targeting, and penetration due to inadequate vasculature and extensive extracellular matrix in the tumor. Thus, overcoming these two limitations of nanotechnology is of considerable interests for cancer researchers. In this dissertation, we demonstrate the feasibility of glucose-modified nanoparticles (GLU-NPs) as an efficient cancer targeted-delivery system for enhancing the systemic circulation time and …
Alternative Treatment Options For Controlling Anthelmintic-Resistant Haemonchus Contortus Populations In South Dakota Sheep Herds, Adam Sarah
Electronic Theses and Dissertations
Haemonchus contortus is a common economically important gastrointestinal nematode that obliges the survival and productivity of small ruminants, especially sheep and goats. Anthelmintic resistance is a primary challenge in ruminant health management programs in preventing and controlling the H. contortus populations; therefore, there is a great necessity in finding and developing natural plant products for use as alternatives to commercial anthelmintics for controlling H. contortus in pastured sheep and goats. This dissertation has three major aims conducted in three separate studies: the first in vivo one was to evaluate the extended effect of a springtime, combined-treatment with 3 anthelmintics having …
Application Of Analytical Techniques In Food Product Development, Sonali R. Pandey
Application Of Analytical Techniques In Food Product Development, Sonali R. Pandey
Electronic Theses and Dissertations
Products are analyzed in major sectors of food industry to meet regulatory standards, food safety, quality control, and research and development. Analytical and extraction techniques have been applied for simultaneous investigation of multiple product characteristics. Food analysis faces critical challenges due to the complex composition of food, leading to matrix effects that result in loss of accuracy and reliability. The analytical techniques require an efficient sample preparation to avoid or minimize matrix effects. Solid-phase extraction has been widely used to address this issue. In this dissertation, the aim is to apply analytical techniques in food product development. To accomplish this, …
Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little
Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little
Electronic Theses and Dissertations
Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …
Winter Snow Depth In Arctic Alaska Results In Complex Changes In Caribou Forage Quality, Jessica C. Richert
Winter Snow Depth In Arctic Alaska Results In Complex Changes In Caribou Forage Quality, Jessica C. Richert
Electronic Theses and Dissertations
Caribou (Rangifer tarandus) rely on the short growing season for much of their annual nutrition, making them susceptible to even small changes in forage quantity and quality. Body condition in the summer and fall is linked to winter survival rates and fecundity in cows, critical factors in the robustness of caribou populations. Due to a warmer, wetter climate, snowfall is predicted to increase over Alaska’s North Slope in the next several decades. Deeper snow results in higher soil temperatures, allowing microbial mineralization of nitrogen to continue throughout the winter and increasing the availability of nitrogen for plants in spring and …
Rumen Microbial Culture Library And In Vitro Analysis Of Selected Bacterial Species On Colonization Resistance Against Bovine Enteric Pathogens, Maria Antony
Electronic Theses and Dissertations
The anaerobic habitat of the four chambers in ruminant’s foregut plays a vital role in the fermentation of the food by the active involvement of the residing microbial community. The cultivability and the potential role of rumen bacteria in the field of livestock production, host health and immunity are important to study. The developed anaerobic culturing techniques enable us to culture and screen different rumen bacteria to study the anti-pathogenic effects. Whole genome sequencing and analysis help us to understand the genome level characteristics of the rumen bacterial species individually. Since the majority of the rumen biosphere is still not …
Finite Mixture Of Regression Models For Complex Survey Data, Abdelbaset Abdalla
Finite Mixture Of Regression Models For Complex Survey Data, Abdelbaset Abdalla
Electronic Theses and Dissertations
Over time, survey data has become an essential source of information for modern society. However, to be effective, the structures of survey data require sampling designs that are more complex than simple random sampling. The complex sampling data collected from enormous national surveys via these complex designs ideally include sample weights that allow analysis to take account of complicated population structures. When the target of inference is the parameters of a regression model, it is crucial to know whether these weights should be incorporated into the sampling weight when fitting the model to the survey data. The finite mixture models …
Searching For A Sense Of Place: The Process Of How Adolescent Girls Overcome Suicidality, Tamara Keefner
Searching For A Sense Of Place: The Process Of How Adolescent Girls Overcome Suicidality, Tamara Keefner
Electronic Theses and Dissertations
Suicide and self-harming behaviors are significant public health problems among children and adolescents globally and in the United States. Despite significant advances in recent decades to understand, prevent, and treat childhood and adolescent self-harming and suicide, suicide rates remain high. Since the National Action Alliance for Suicide Prevention set a goal to reduce suicide deaths and attempts by 40% by 2020, there has been a paradigm shift in the study of suicide yielding significant advances in the understanding of suicide and other forms of self-inflicted injury. This dissertation highlights some of the new developments in the study of suicide. First, …
Sunflower Pollinators In South Dakota: An Evaluation Of Species Composition, Abundance And Influence On Yield, Mackenzie Mattern
Sunflower Pollinators In South Dakota: An Evaluation Of Species Composition, Abundance And Influence On Yield, Mackenzie Mattern
Electronic Theses and Dissertations
In 2017 and 2018, South Dakota was the largest producer of sunflowers, Helianthus annuus L. (Asterales: Asteraceae) in the US, grossing approximately $178 million per year. In South Dakota, both confection and oilseed sunflower cultivars are grown. Despite breeding efforts to increase sunflower's ability to self-pollinate there is evidence that pollinator visitation results in increased sunflower yields. In addition, there is evidence that sunflower pollen is beneficial for pathogen resistance in various bee species, suggesting the importance of a mutualistic relationship between pollinators and sunflowers. In some areas, observed increases in sunflower yield is attributed to the presence of Apis …
The Impacts Of Land Use And Land Cover Change On Water Quality In The Big Sioux River: 2007-2016, Dinesh Shrestha
The Impacts Of Land Use And Land Cover Change On Water Quality In The Big Sioux River: 2007-2016, Dinesh Shrestha
Electronic Theses and Dissertations
Between 2006 and 2012, conversion of 485,000 acres of grassland to cropland in eastern South Dakota was reported. In 2012, the Big Sioux River (BSR) running through most of eastern South Dakota was listed among the dirtiest rivers in the nation. This rating convinced state authorities to study trends of land cover changes in the BSR watershed and its association with BSR water quality with respect to increases in nitrate levels. This research i) quantifies spatial and temporal changes in the land cover types within the BSR watershed, and ii) identifies any correlation between these changes and changes in BSR …
Development Of A Neural Network-Based Object Detection For Multirotor Target Tracking, Spencer Harwood
Development Of A Neural Network-Based Object Detection For Multirotor Target Tracking, Spencer Harwood
Electronic Theses and Dissertations
Unmanned aerial vehicles (UAVs) have, for the past few decades, seen an increased popularity in industry and research centres. Despite this intense utilization by both markets there exists an active demand for the development of autonomous guidance, navigation, and control strategies. One need relates to the achievement of a high level of autonomy to identify and track a target object. An elective technique for this set of tasks is neural networks. In the development and study of these networks there is a distinct lack of substantive validation techniques to qualify network performances when implemented in a multirotor UAV. This thesis …