Digital Commons Network^™

Social Determinants Of Health Theory: Policy Entry Points For Healthcare Providers, Kiah R. Vandergrift

Electronic Theses and Dissertations

This thesis examines a shifting paradigm in the U.S. healthcare system that will re-define the role that the healthcare provider plays in the production of health. It does this by first acknowledging two differing economic theories on the production of health: Human Capital Theory and Social Determinants of Health theory, and how both schools of thought have contributed to the paradigm that healthcare providers only play a role in the end stages of health production - to heal those who are already ill. However, policy, payment, and delivery system reforms have begun to force healthcare providers to re-think how they …

An Archaeological Investigation Into Social Organization And Political Reform In The Reserve Area Of New Mexico, A.D. 1000–1350, Cameron D. Benton

Electronic Theses and Dissertations

This thesis analyzes archival data from archaeological sites with great kivas in the Reserve region of west-central New Mexico dating to A.D. 1000-1350 and examines sociopolitical organization and reform between the dynamic Reserve (A.D. 1000-1100) and Tularosa (1100-1350) Phases. Specifically, studies in this thesis compare great kiva architecture and ceramic types present between sites using methods of descriptive statistics and quantitative analysis, which allowed for interregional variation and change to be identified between those time periods. The results of those analyses are correlated with the archaeological histories of the Mimbres and Chaco societies that bordered the Reserve area in prehistory …

Characterization Of A Phosphomimetic Mutant Of The Als Associated Protein Tdp-43, Nicole Toro

Electronic Theses and Dissertations

Trans-activation response (TAR) DNA-binding protein 43 (TDP-43) is a natively dimeric 414-residue protein that is encoded by the human TARDBP gene that has important implications in the pathogenesis of the neurodegenerative disorders ALS, FTD, and CTE. TDP-43 has been found hyperphosphorylated and ubiquitinated in the aggregates of the affected neurons of these diseases. The discovery of the presence of TDP-43 positive inclusions in brain matter of patients with CTE has made repetitive brain injury a possible environmental stimulus for aggregation in TDP-43 proteinopathies. We expand upon the hypothesis that TDP-43 readily aggregates under agitation conditions and that the addition of …

Transgenerational Effects Of Maternal Age On Offspring Fitness In Crickets, Jacob D. Wilson

Electronic Theses and Dissertations

Advanced parental age is an important aspect of parental condition that can have both positive and negative effects on offspring fitness, and thus, parental age can be considered a parental effect. As a parental effect, parental age may affect a variety of offspring traits and may cascade to influence several generations of offspring. Given the complexities of studying both paternal and maternal age, we studied the effects of maternal age only. Using the Pacific field cricket, Teleogryllus oceanicus, we asked 1) does maternal age have influences over several generations of offspring and 2) does maternal age influence the reproductive …

Exploring The Lived Religious Experiences Of Gay And Lesbian Ordained Clergy In The United Methodist Church, Tracy Allen Temple

Electronic Theses and Dissertations

The United Methodist Church (UMC) as an institution has strict language against lesbian and gay people and against the ordination of the lesbian and gay people. Prior research has focused on how denominations and congregations discuss issues around sexuality, how attitudes have shifted around issues of sexuality, and how to provide spiritual care for the LGBTQ+ community. This qualitative study interviewed lesbian and gay ordained elders in the UMC to learn about their experiences of serving as clergy people in the UMC even though there are prohibitive statements in church documents. Firstly, the interviews revealed that the participants feel loved …

Rapid-Scan Epr And Imaging, Yilin Shi

Electronic Theses and Dissertations

EPR imaging at low frequency is a powerful tool to obtain important biological information in vivo in a non-invasive way. Properties of nitroxide and trityl radical imaging reagents have been studied. Developments in rapid scan imaging techniques are reported that improve efficiency of experiments and user-friendliness of software.

Relaxation and signal-to-noise ratio (S/N) in pulse experiments on trityl radicals were measured at frequencies between 400 MHz and 1.5 GHz. Relaxation time increases as the frequency increases and the radical concentration decreases. Since relaxation time is a sensitive and accurate measure of oxygen pressure, this study provides criteria for the selection …

Unraveling Pdi And Its Interaction With Ab Toxins, Jessica Guyette

Electronic Theses and Dissertations

Protein disulfide isomerase (PDI) is an essential endoplasmic reticulum (ER) protein that acts as both an oxidoreductase and chaperone. It exhibits substantial flexibility and undergoes cycles of unfolding and refolding in its interaction with cholera toxin (Ctx), which is a unique property of PDI. This unfolding allows PDI to disassemble the Ctx holotoxin, which is required for Ctx activity. Here, we investigated the unfolding and refolding property of PDI and how this affects its interaction with bacterial toxins. PDI showed remarkable redox-linked conformational resilience that allows it to refold after being thermally stressed. Deletion constructs of PDI showed that both …

Parameter Estimation Of Stochastic Models Against Probabilistic Temporal Logic Behavioral Specifications, Arfeen Khalid

Electronic Theses and Dissertations

The inherent behavioral variability exhibited by stochastic systems makes it a challenging task for human experts to manually analyze them. Computational modeling of such systems helps in investigating and predicting the behaviors of their underlying processes but at the same time introduces the presence of several unknown parameters. A key challenge faced in this scenario is to determine the values of these unknown parameters against known behavioral specifications. The solutions that have been presented so far estimate the parameters of a given model against a single specification whereas a correct model is expected to satisfy all the behavioral specifications when …

A Mechanistic Study Of The Teratogenic Potential Of 4-O-Methylhonokiol (Mh) On Japanese Medaka (Oryzias Latipes), Santu Kumar Singha

Electronic Theses and Dissertations

An additional goal of this project was to characterize the Wnt/β-catenin signaling pathway to unravel the adverse potential of MH on the cardiovascular system (CVS). Our study suggested that MH-mediated cardiovascular injury may be caused due to upregulation of some major components of the Wnt/β-catenin pathway through expression of excessive pro-inflammatory mediators. Moreover MH decreased expression of ErbB3 and NRG-2 suggesting impaired cardiac structure. MH exposure caused a slow heartbeat blood occlusion absence of blood circulation decreased hatching efficiency and increased mortality in medaka embryos in a concentration- and time-dependent manner. MH might cause blood vessel occlusion by increasing the …

Examining The Role Of Reproductive Interference In The Decline Of Native Green Treefrogs Following Cuban Treefrog Invasions, Joseph Kennedy

Electronic Theses and Dissertations

Reproductive interference occurs when the mating behaviors of one species negatively impact the fitness of another species. It is of increasing interest in invasive species biology because the introduction of alien species often leads to novel sexual interactions with native taxa, which can contribute to their decline. I examined whether reproductive interference plays a role in the decline of native green treefrogs (Hyla cinerea) following invasions of Cuban treefrogs (Osteopilus septentrionalis) in the southeastern United States. The impetus for this study revolves around similarities in spectro-temporal features of Cuban treefrog courtship calls and green treefrog aggressive calls. The significance of …

Safety Assessment And Chemical Fingerprint Analysis Of Medicinal Plants Commonly Consumed As Herbal Teas; Rooibos (Aspalathus Linearis) And Moringa (Moringa Oleifera), Omer I. Fantoukh

Electronic Theses and Dissertations

The first chapter discusses the significance of natural products, safety challenges, and quality issues concisely. Specifically, the impact of herb-drug interaction on consumers and the quest for chemical markers for chemical fingerprinting development are provided. Rooibos tea (Aspalathus linearis) is a popular South African herbal tea enjoyed worldwide. Limited reports indicate the potential of rooibos tea to alter the activity of CYP450 isozymes. In chapter two, the phytochemical investigation of A. linearis resulted in the isolation and characterization of 14 phenolic compounds. Safety assessment of the phytochemicals through interaction with CYP, PXR, and P gp was conducted. The …

Identifying Transcription Co-Regulators Of Gcm/Repo In The Development Of Glia In Drosophila Melanogaster, Glendin Kostantinos Pano

Electronic Theses and Dissertations

The molecular mechanism mediating the differentiation of neural progenitors to either glia or neurons is guided by the gene glial cells missing (gcm), a master regulator of glial cell differentiation. Knockout of this transcription factor (TF) and its homolog gcm2 in the fruit fly Drosophila Melanogaster results in embryos that develop neurons in place of lateral glia. Conversely, overexpression of gcm in those neural progenitors results in embryos that have substantially fewer neurons and excess glia(Jones, et.al, 1995; Hosoya, et.al, 1995; and Vincent, et.al, 1996). Additionally, gcm has also been shown to be involved in the differentiation of blood …

Latinx Student Success In Higher Education: Genres, Racialization, And Generic-Racial Interactions., Delia Garcia

Electronic Theses and Dissertations

Using a transformative mixed methods approach, I explored the ways in which genres and racial projects are constitutive of each other and contribute to the racialization of Latinx students in higher education and result in disparate outcomes for Latinx students. Latinx research participants revealed the myriad ways in which they are racialized throughout the education system and the numerous ways they encounter whiteness on a daily basis. I focused on two genres in particular: the federally mandated race and ethnicity categories and their intersections with Big Data/Predictive Analytics (BDPA) projects at the university. Rhetorical Genre Studies (RGS) and Racial Formation …

Collaborative Artificial Intelligence Algorithms For Medical Imaging Applications, Naji Khosravan

Electronic Theses and Dissertations

In this dissertation, we propose novel machine learning algorithms for high-risk medical imaging applications. Specifically, we tackle current challenges in radiology screening process and introduce cutting-edge methods for image-based diagnosis, detection and segmentation. We incorporate expert knowledge through eye-tracking, making the whole process human-centered. This dissertation contributes to machine learning, computer vision, and medical imaging research by: 1) introducing a mathematical formulation of radiologists level of attention, and sparsifying their gaze data for a better extraction and comparison of search patterns. 2) proposing novel, local and global, image analysis algorithms. Imaging based diagnosis and pattern analysis are "high-risk" Artificial Intelligence …

Characterization Of Rare-Earth Doped Thermal Barrier Coatings For Phosphor Thermometry, Quentin Fouliard

Electronic Theses and Dissertations

Thermal Barrier Coatings have been extensively used to protect and insulate the metallic components in turbine engines from extreme environments to achieve higher turbine inlet temperatures, resulting in an increase in combined engine cycle efficiency, lowering NOx emissions and fuel consumption. Additionally, as the major failure mechanisms determining lifetime are thermally activated during engine operation, uncertainty in temperature measurements affects lifetime prediction. Early detection of Thermal Barrier Coatings spallation symptoms, associated with local delamination induced temperature changes, can reduce forced engine outages. Further improvements are envisioned with the development of more reliable measurement techniques. For this purpose, Phosphor Thermometry has …

Metabolic Effects Of 17a-Estradiol Are Growth Hormone Independent And Sex Specific, Silvana Sidhom

Electronic Theses and Dissertations

Aging is a major risk factor for metabolic syndromes and type two diabetes. With growing elderly populations worldwide and increasing incidence of age-related diseases there is a great need to develop pharmacological interventions that would delay aging and protect from age-related diseases. 17-alpha estradiol (17α-E2) is an epimer of the primary female sex hormone estradiol and has been shown to extend lifespan and downregulate markers of age-related metabolic dysfunction in male mice. Because 17α-E2 does not induce feminization in males it holds potential as a novel therapeutic in humans for age-related metabolic dysfunction. Importantly, we have previously shown that 17α-E2 …

Mechanism Of Nanogp8 In Glioblastoma Multiforme, Jonhoi Smith

Electronic Theses and Dissertations

Glioblastoma multiforme (GBM) is an incurable brain tumor, with patients only expecting to live 15 to 16 months post-diagnosis with the most current treatments options including surgery, chemotherapy, and radiation. How GBM resist treatment is still not very well known. However, cancer stem cells (CSCs), a subset of cells in GBM tissue considered responsible for therapeutic resistance and the poor patient prognosis. NANOG, a homeobox gene, is responsible for maintaining pluripotency of embryonic stem cells and is observed in CSCs. NANOGP8, a retrogene in the NANOG family is expressed in CSCs and provides cells with stem-like characteristics previously observed in …

Identification And Functional Characterization Of A Long Non-Coding Rna Associated With Prostate Cancer, Md Faqrul Hasan

Electronic Theses and Dissertations

Prostate cancer is the most common cancer in men in the western world. Although early stage prostate cancer is treatable late stage, more specifically, metastatic and drug resistant prostate cancers are mostly incurable. The failure of current treatments obligates the research community to explore novel areas in prostate cancer biology and find better therapeutic targets. Emerging evidences show that non-coding RNAs specifically long non-coding RNAs (lncRNAs) play regulatory roles in various cellular processes and are frequently dysregulated in cancer including prostate cancer. These aberrantly expressed lncRNAs mostly with unexplored genetic information may drive cancer progression. Previous studies done in our …

Development Of A Functional Testing Platform For The Sensory Segment Of The Neuromuscular Reflex Arc, Alisha Colon

Electronic Theses and Dissertations

Investigations of human biology and disease have been hindered by the use of animal models. The information obtained from such studies often results in clinically irrelevant results and drug trial failures. Additionally, several governing bodies have been formulating legislation to move away from animal models and toward more ethical and efficient testing platforms for drug discovery and cosmetic research. As an answer to these issues, "body-on-a-chip" systems have been a rapidly developing field which easily recapitulates in vivo functionality, providing a more relevant, repeatable, and ethical testing platform to better predict biology. These systems can be used as human-based testing …

Filling The Gaps: Common Bottlenose Dolphin (Tursiops Truncatus) Population Dynamics, Structure, And Connectivity Within Florida Panhandle Bays, Sounds, And Estuaries, Christina Toms

Electronic Theses and Dissertations

At the time of the Deepwater Horizon oil spill in 2010, very little was known about the inshore bottlenose dolphin (Tursiops truncatus) populations hit hardest by the spill. Without previous population demographic data impacts were challenging to assess. My dissertation was designed to build research capacity moving forward by helping to fill data gaps in the western Florida Panhandle. The first study presented here resulted in the first system-wide assessment of seasonal abundance, survival, and site fidelity patterns of bottlenose dolphins that inhabit the Pensacola Bay system. In contrast to a previous study that reported only 33 dolphins, results from …

Biomass Density Based Adjustment Of Lidar-Derived Digital Elevation Models: A Machine Learning Approach, Khalid Abdelwahab

Electronic Theses and Dissertations

Salt marshes are valued for providing protective and non-protective ecosystem services. Accurate digital elevation models (DEMs) in salt marshes are crucial for modeling storm surges and determining the initial DEM elevations for modelling marsh evolution. Due to high biomass density, lidar DEMs in coastal wetlands are seldom reliable. In an aim to reduce lidar-derived DEM error, several multilinear regression and random forest models were developed and tested to estimate biomass density in the salt marshes near Saint Marks Lighthouse in Crawfordville, Florida. Between summer of 2017 and spring of 2018, two field trips were conducted to acquire true elevation and …

An Assessment Of Biosorption Activated Media For The Removal Of Pollutants In Up-Flow Stormwater Treatment Systems, Andrew Hood

Electronic Theses and Dissertations

Nitrogen and phosphorus are often the limiting nutrients for marine and freshwater systems respectively. Additionally, stormwater often contains elevated levels of pathogens which can pollute the receiving water body and impact reuse applications [1-4]. The reduction of limiting nutrients and pathogens is a common primary target for stormwater best management practices (BMPs) [5]. Traditional BMPs, such as retention/detention treatment ponds require large footprints and may not be practical in ultra-urban environments where above ground space is limited. Upflow filters utilizing biosorption activated media (BAM) that can be placed underground offer a small footprint alternative. Additionally, BAM upflow filters can be …

Development Of In Vitro Point Of Care Diagnostics (Ivpcd) Based On Aptamers Integrated Biosensors, Nileshi Saraf

Electronic Theses and Dissertations

The global market for the medical diagnostic industry is worth 25 billion dollars in the United States and is expected to grow exponentially each year. Presently available methods for biodetection, such as immunoassays, chemiluminescence and fluorescent based assays are expensive, time consuming and require skilled labor with high-end instruments. Therefore, development of novel, passive colorimetric sensors and diagnostic technologies for detection and surveillance is of utmost importance especially in resource constrained communities. The present work focusses on developing novel and advanced in vitro biodiagnostic tools based on aptamer integrated biosensors for an early detection of specific viral proteins or small …

A Theoretical, Numerical, And Experimental Study Of Trapped Droplet Coalescence And Mixing, Passively And Under Electric Field, Alireza Karbalaei Baba

Electronic Theses and Dissertations

Passive and electrically active coalescence and mixing of pairs of trapped squeezed nanodroplets were studied in this work. PDMS-glass microfluidic devices were designed and fabricated using multilevel photolithography technique. Flow-focusing method was used to generate nanoliter droplets of died glycerol inside oleic acid. The effect of factors such as flow rates and their ratio, interfacial tension, and viscosities on the size and frequency of droplet generation was studied and concluded by demonstrating the capillary number effect. A passive droplet trapping technique based on minimizing the surface energy of the droplets was employed to minimize the shear flow effects and increase …

Investigation Of Coastal Vegetation Dynamics And Persistence In Response To Hydrologic And Climatic Events Using Remote Sensing, Subrina Tahsin

Electronic Theses and Dissertations

Coastal Wetlands (CW) provide numerous imperative functions and provide an economic base for human societies. Therefore, it is imperative to track and quantify both short and long-term changes in these systems. In this dissertation, CW dynamics related to hydro-meteorological signals were investigated using a series of LANDSAT-derived normalized difference vegetation index (NDVI) data and hydro-meteorological time-series data in Apalachicola Bay, Florida, from 1984 to 2015. NDVI in forested wetlands exhibited more persistence compared to that for scrub and emergent wetlands. NDVI fluctuations generally lagged temperature by approximately three months, and water level by approximately two months. This analysis provided insight …

Waking Up White: The Search For Black Happiness, Jason Gregory

Electronic Theses and Dissertations

Waking Up White (WUW) is a television pilot written, directed and co-produced by Jason D. Gregory serving as part of his thesis requirements for earning a Master of Fine Arts in the Entrepreneurial Digital Cinema program at the University of Central Florida. The project attempts to answer the question "what if" by placing the main characters in a "fish out of water" scenario. In WUW a Black family wakes up White and has 30 days to determine if they'd rather return to their cultural roots or stick with the "new skin" that they are in. Upon awakening to their new …

Cavitation And Heat Transfer Over Micro Pin Fins, Arash Nayebzadeh

Electronic Theses and Dissertations

With the dramatic increase in the usage of compact yet more powerful electronic devices, advanced cooling technologies are required to maintain delicate electronic components below their maximum allowable temperatures and prevent them from failure. One solution is to use innovative pin finned heat sinks. This research is centered on the evaluation of hydrodynamic cavitation properties downstream pin fins and extended toward single-phase heat transfer enhancement of array of pin fins in microchannel. In this work, transparent micro-devices capable of local wall temperature measurements were micro fabricated and tested. Various experimental methods, numerical modeling and advanced data processing techniques are presented. …

Disinfection By-Product Formation Potential Assessment For Central Florida Groundwater Supplies, Tulsi Shukla

Electronic Theses and Dissertations

Disinfection of potable water supplies is a primary requirement of the United States' Environmental Protection Agency's (USEPA's) Safe Drinking Water Act. The use of chlorine as a disinfectant is widely accepted by water purveyors due to its effectiveness and low cost. However, chlorine reacts with natural organic matter present in water supplies to form suspected carcinogenic disinfection by-products (DBPs). In this work, the formation of the regulated chlorinated by-products total trihalomethanes (TTHMs) and haloacetic acids (HAA5) for eleven Central Florida wells has been investigated. Fluorescence, UV-254, dissolved organic carbon (DOC), specific UV absorbance (SUVA), chlorine decay, and TTHM and HAA5 …

Nanoscale Functional Imaging By Tailoring Light-Matter Interaction To Explore Organic And Biological Systems, Negar Otrooshi

Electronic Theses and Dissertations

Probing molecular systems with light has been critical to deepen our understanding of life sciences. However, conventional analytical methods fail to resolve small quantities of molecules or the heterogeneity in molecules assembled into complex systems. This bottleneck is mostly attributed to light diffraction limit. In recent years, the successful implementation of new approaches to achieve sub-wavelength chemical speciation with an Atomic Force Microscope (AFM) has paved the way to a deeper understanding of the effect of local composition and structure on the functional properties of a larger scale system. The combination of infrared light, to excite the vibrational modes of …

Some Studies On Leaf Spot Of Oats And Triticale, Mohammed Abdullah Lashram

Electronic Theses and Dissertations

Oat is an important cereal crop, and it is considered to be among the highest used in cereal crops. It is considered among the healthiest grains due to the rich source of the soluble fiber, β-glucan that helps in lowering the cholesterol. Oat production and demand have increased considerably in the past few years due to its health benefits. South Dakota is ranked in the top three largest oat-producing states in the USA. Oat is relatively less susceptible to pest and diseases except for leaf diseases such as crown rust, Drechslera avenae leaf spot, and Stagonospora avenae leaf blotch. Leaf …