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Rab39 And Klp98a Are Required For Furrow Formation During Early Drosophila Embryogenesis, Megan R. Millage

Electronic Theses and Dissertations

The formation of a plasma membrane furrow is an essential process during development. Furrow formation is necessary for successful cell division and cytokinesis in addition to the ability to create multicellular tissues. Here, I will explore the role of the Golgi-associated Rab protein Rab39 in furrow formation during early Drosophila embryogenesis. Rab39 is one of eight Rab proteins that has been shown to localize to discrete puncta by live imaging in early Drosophila embryos, but its function and pathway have not been well characterized. In this thesis will I show that Rab39 forms dynamic, tubular structures that colocalize with trans-Golgi …

Youth With Juvenile Justice Contact: Special Considerations In Measurement, Anne Elizabeth Biehl

Electronic Theses and Dissertations

Students who have contact with the juvenile justice system are a particularly vulnerable and unique school-aged population. The risk factors that plague the likelihood of justice-involvement are numerous and inter-connected. Early experiences of trauma and adversity, limited familial and financial capital, and challenges with mental health all contribute to increased likelihood of youth contact with juvenile justice systems. Despite said risk factors effects on young people overall, youth of color are particularly susceptible to become justice-involved. School and community discipline statistics are grossly, racially disproportionate.

Pathways from schools to the justice system have been widely investigate in the literature. There …

Pivotal Perceptions: A Phenomenological Exploration Of Trauma-Informed Practices In An Urban School, Marni Choice-Hermosillo

Electronic Theses and Dissertations

This phenomenological study sought to examine the experiences of teachers in an urban K-8 school after a system-wide whole school implementation of trauma-informed practices. The practices teachers implemented in their classrooms that aligned with their personal perceptions of trauma-informed practices and its efficacy were explored. Additionally, the personal and professional barriers to implementation were also investigated. Identified practical strategies at both the elementary and middle school levels included establishing and maintaining relational trust and classroom community, actively teaching emotional regulation skills, and teaching and reinforcing rituals, routines and expectations throughout the school year. Lack of confidence and previous personal assumptions …

Renewable Energy Integration In Distribution System With Artificial Intelligence, Yi Gu

Electronic Theses and Dissertations

With the increasing attention of renewable energy development in distribution power system, artificial intelligence (AI) can play an indispensiable role. In this thesis, a series of artificial intelligence based methods are studied and implemented to further enhance the performance of power system operation and control.

Due to the large volume of heterogeneous data provided by both the customer and the grid side, a big data visualization platform is built to feature out the hidden useful knowledge for smart grid (SG) operation, control and situation awareness. An open source cluster calculation framework with Apache Spark is used to discover big data …

Sorting Of Cargo Proteins Within The Regulated Secretory Pathway: The Peripheral Membrane Protein Hid-1 As A Sorting And Vesicle Biogenesis Factor, Blake H. Hummer

Electronic Theses and Dissertations

Large dense core vesicles (LDCVs) form at the trans-Golgi network (TGN) and mediate the regulated release of neuropeptides and peptide hormones. Despite their central role to physiology, the mechanisms controlling biogenesis and sorting to LDCVs is not well understood. Optimizing the retention using selective hooks (RUSH) method in neuroendocrine cells, we show it is possible to visualize sorting to the constitutive and regulated secretory pathways in real-time and that the bulk of transmembrane LDCV cargoes do not sort directly onto LDCVs, but exit the TGN into non-regulated vesicles to be incorporated to LDCVs at a later step. Additionally, we characterize …

A Qualitative Study Of Non-Relational Foster Families Experiences Navigating The Education System, Emma Grace Topf

Electronic Theses and Dissertations

This qualitative study used a phenomenological approach to capture the lived experience of non-relational foster families navigating the Colorado public education system. While most research on children in foster care looks at all sub-populations of foster care combined, this is the first known study to focus solely on non-relational foster families navigating the education system. I explored non-relational foster parents experience navigating the education system for the school-age children in their care through a semi-structured two-interview approach. Four levels of data analysis were completed (descriptive coding, emergent coding of themes, a priori coding of research questions, and a priori coding …

Sensitivity Of Radical Sam Enzyme Mftc To Molecular Oxygen, Vishnu Govindarajan

Electronic Theses and Dissertations

MftC is a radical S-adenosyl L-methionine (SAM) enzyme that catalyzes the first step in the production of mycofactocin. It is a ribosomally synthesized post-translationally modified (RiPP) redox cofactor shown to be essential for the survival of bacteria in the Mycobacterium genus in the presence of cholesterol as a carbon source and for the sequestration of ethanol. MftC catalyzes the C – terminal decarboxylation of tyrosine and the subsequent cross-linking of tyrosine to the penultimate valine on the precursor peptide MftA. The product formed out of the reaction is processed into mycofactocin in downstream processes. The bacteria in the Mycobacterium tuberculosis …

Quantification Of Dynamic Epithelial Sheet Architecture In Botryllus Schlosseri Using 2-D & 3-D Image Analysis, Roopa Madhu

Electronic Theses and Dissertations

Epithelial tubules form critical structures in various body tissues; how- ever, since they are difficult to access experimentally, their architecture and dynamics are not well understood. Here we examine the dynamic remodeling of epithelial tubes in vivo using a novel and uniquely accessible model system: the extracorporeal vasculature of Botryllus schlosseri (sea squirt). In Botryllus, massive retraction of blood vessels can be triggered without loss of barrier function, through (i) disrupting collagen crosslinking in the basement membrane using β-aminopropionitrile (BAPN); or (ii) disrupting the integrin pathway through inhibition of focal adhesion kinase (FAK). We performed stereographic projections of 3-dimensional …

Rock Glacier Development In The San Juan Mountains, Brandon K. Bailey

Electronic Theses and Dissertations

Rock glaciers are common landform features found in deglaciated alpine areas. They are commonly used in the study of climatic changes throughout the Holocene and the reconstruction of neoglacial chronologies. For this research, Schmidt hammer rebound values, weathering rind thicknesses, and the length of lichen thalli diameters found on rock glacier surfaces are used to investigate their effectiveness as field-based relative age determination techniques. Additionally, the ability to identify periods of neoglacial activity using these methods is assessed in two neighboring cirque basins in the San Juan Mountains of Colorado. 41 field sites across three rock glaciers are established with …

Working For A Living: A Terror Management Theory Approach To Finding Meaning In Vocation, Julian T. Frazier

Electronic Theses and Dissertations

Many individuals spend approximately a third of their lives either working, receiving training or education for work or otherwise engaged in their career. While the current literature attempts to discern the many roles that work can play in our lives, it only scantly explore the existential nature of work in relation to death and mortality. Terror Management Theory provides a framework explaining how increases in awareness of our mortality influences our behavior and beliefs. By studying work constructs from the lens of Terror Management Theory, we seek to gain insight on the potential role that work plays in bolstering psychological …

Automated Recognition Of Facial Affect Using Deep Neural Networks, Behzad Hasani

Electronic Theses and Dissertations

Automated Facial Expression Recognition (FER) has been a topic of study in the field of computer vision and machine learning for decades. In spite of efforts made to improve the accuracy of FER systems, existing methods still are not generalizable and accurate enough for use in real-world applications. Many of the traditional methods use hand-crafted (a.k.a. engineered) features for representation of facial images. However, these methods often require rigorous hyper-parameter tuning to achieve favorable results.

Recently, Deep Neural Networks (DNNs) have shown to outperform traditional methods in visual object recognition. DNNs require huge data as well as powerful computing units …

Performances Of An Able, Academic Mind, Caleb Green

Electronic Theses and Dissertations

Western culture individualizes issues of public health. This is especially clear in academic life, where the structures of the university disable atypical bodies and minds in order to force them to simultaneously perform the roles of scholar, teacher, and colleague. The university not only fails to accommodate afflicted minds and bodies, it also produces more precarity in the process. This project is a performance ethnography of my time in the academy, starting with my life as an undergraduate being disciplined into academic life, moving toward recruitment for graduate school, and ending with events surrounding the construction of this very project. …

Using Multi-Source Data To Assess The Dynamics Of Socioeconomic Development In Africa, Xuantong Wang

Electronic Theses and Dissertations

Frequent and rapid spatially explicit assessment of socioeconomic development is critical for achieving the Sustainable Development Goals (SDGs) at both national and global levels. In the past decades, scientists have proposed many methods for monitoring human activities on the Earth’s surface on various spatiotemporal scales using Defense Meteorological Satellite Program Operational Line System (DMSP-OLS) nighttime lights (NTL) data. However, the DMSP-OLS NTL data and the associated processing methods have limited their reliability and applicability for systematic measuring and mapping of socioeconomic development. This research utilizes Visible Infrared Imaging Radiometer Suite (VIIRS) NTL and the Isolation Forest (iForest) machine learning algorithm …

The Casc Framework: An Assessment Model For Students With Intellectual And Developmental Disabilities, Laurel A. Snider

Electronic Theses and Dissertations

School psychologists pay a critical role in providing assessment and intervention services within the realm of special education. Within this role, they are highly likely to interact with students with intellectual and developmental disabilities (IDD). Students with IDD are characterized by significantly lower than average cognitive and daily living skills that may be comorbid with difficulties with communication, social skills, or other domains. With these differences in mind, dominant assessment practices have been criticized as lacking social and empirical validity when applied to this population. Although students with IDD frequently undergo evaluations, they continue to face significantly poorer post-school outcomes …

Cellular And Organismal Ramifications Of De Novo Purine Synthesis Dysregulation, Randall Craig Mazzarino

Electronic Theses and Dissertations

Purines are a class of nitrogenous bases and are essential small molecules to life. Purines are used within the cell as genetic information carriers, energy currency, signaling molecules, and cofactors for multiple processes. They are formed through de novo and salvage pathways found in cells across the phylogenetic tree. The substrates of enzymes within de novo purine synthesis are known to influence other processes within the cell, such as energy homeostasis. In humans, de novo purine synthesis disorders are rare, with around 100 people identified. These patients exhibit a range of phenotypes, with varying degrees of mental retardation, seizure activity, …

How 6-12th Grade Staff Support Students With Depression: A Pilot Study To Develop Measures Of Implicit Associations, Explicit Attitudes And Helping Behavior, Paul M. Thompson

Electronic Theses and Dissertations

Students with emotional disabilities are disproportionately suspended and expelled in K-12 schools. Attribution theory suggests individuals are less likely to provide assistance to others if they believe the individuals are responsible for their own difficulties. To test attribution theory, this study created new measures of explicit attitudes and implicit associations of licensed 6-12th grade staff regarding students with depression as well as a helping behavior measure of staff toward students with depression. The survey was distributed within a single school district in the western United States. A majority of the sample (N = 52) held a mental health license (60%), …

Social Distance, Stigma, And Help-Seeking: An Exploration Of Autism Beliefs In The Southern United States, Christian Shannon Courson

Electronic Theses and Dissertations

Stigma has been associated with physical and mental health conditions and their treatment long before formal care systems. Negative perceptions within the lay community, such as stigma and social distancing, negatively impact individuals with autism and their families. The purpose of this study was to explore factors associated with autism stigma among laypeople in the Southern United States. Specifically, the study explored how demographics, knowledge, and familiarity are related to social distancing and stigma, and how these factors relate to beliefs about and intentions of help-seeking. Using a lay sample (N=108) of rural Southern towns, survey data was collected in …

Formulation Development Of Curcumin-Piperine Solid Dispersion Via Hot-Melt Extrusion To Enhance Solubility And Bioavailability Of Curcumin, Abdulmajeed Althobaiti

Electronic Theses and Dissertations

Curcumin has been known to have a variety of biological activities in different diseases, including inflammation, cancer, and diabetes. The limited bioavailability of curcumin is due to its low solubility and extensive metabolism, rendering curcumin unusable as an oral therapeutic agent. The aim of the study is to enhance the solubility and bioavailability of curcumin by preparing curcumin-piperine solid dispersion (SD) utilizing the hot-melt extrusion technique. Three physical mixtures with different ratios were prepared as following: F1 (5% w/w piperine, 15% w/w curcumin, 80% w/w Soluplus®), F2 (10% w/w piperine, 30% w/w curcumin, 60% w/w Soluplus®), and F3 (15% w/w …

Examining Communication Patterns And Identity In Families With Children Who Are Deaf Or Hard Of Hearing, Amber L. Alvey

Electronic Theses and Dissertations

“Deafness in the context of the hearing family attacks the backbone of the family structure, namely communication” (Ahlert & Greeff, 2012, p. 402). There is a link between communication and identity, specifically the role of communication on identity (Jung & Hecht, 2004). The purpose of this study is to better understand the experiences of families with children who are deaf or hard of hearing by investigating the relationship between family communication patterns and identity. In this study, I conducted in-depth interviews with the parents of children who are hard of hearing to investigate family communication patterns and identity. Using Family …

In Situ Analysis Of Line-1 Promoter Activity Using Lacz Transgenic Mice, Partha Sarathi Saha

Electronic Theses and Dissertations

Apart from an evolutionary role, transposable elements have been implicated in animal development and also in pathophysiology. Non-LTR retrotransposons– LINE-1, Alu and SVA - are responsible for over 120 cases of human genetic diseases as heritable insertions, and are emerging as an important etiological factor for cancer and neurological disorders as somatic mutations. It is estimated that among the total number of 500,000 LINE-1s presents in the human genome, 80-100 LINE-1s remain competent for retrotransposition. Retrotransposition is only possible when LINE-1 is expressed. Because LINE-1 transcription is regulated by its 5’UTR promoter, it is essential to understand the spatiotemporal control …

Mechanistic Studies To Understand The Role Of Human Gut Microbiota In Colonization Resistance And Gut Health, Sudeep Ghimire

Electronic Theses and Dissertations

Human gut microbiota is comprised of thousands of species which fall into two major categories or “enterotypes” based on dominating bacteria: “Bacteroides” and “Prevotella”. Bacteroides enterotype dominates the microbiota of the western population because of a high protein and fat diet, whereas Prevotella enterotype is dominate in the gut of the eastern population because of a high carbohydrate diet. While most of the microbiota studies focused on the Bacteroides enterotype using both metagenomics and culturomics, Prevotella enterotype is understudied, especially in the field of culturomics. The structure of the Prevotella dominated gut microbiome is revealed by …

Genomic And Culturomic Analysis Of Gut Microbiota Function And Salmonella Enterica Expansion, Gavin Fenske

Electronic Theses and Dissertations

Enteric bacteria that are resident in the hindgut of mammals are critical in immune development, digestion, and colonization resistance against pathogens. One of the major pathogens that gut commensals provide resistance against is Salmonella enterica, a major foodborne pathogen capable of infecting almost every warm-blooded animal. Given the interplay between pathogens and commensals in the gut lumen, the gut microbiota of pigs was studied by combining two disparate techniques: shotgun metagenomics and high throughput culturomics. Metagenomics readily identifies major taxa present in samples and can give an estimation to total genetic catalogue from an environment. However, many rare or …

A Natural Antimicrobial From Bacillus Subtilis As A Biosanitizer For Resilient Membrane Biofilms, Pratishtha Verma

Electronic Theses and Dissertations

The microbial attachment and colonization on separation membranes lead to biofilm formation. Some selective isolates within the biofilm constitutive microflora acquire resistance and emerge predominant over prolonged use of the membrane. Thus, proving the cleaning and sanitization protocols to be ineffective in adequately removing resilient biofilms. This subsequently leads to selecting microbial resistance within the constitutive microflora to almost all antimicrobial treatments and hence, creates a need to develop novel alternative strategies to control biofilm formation on membrane surfaces. The first research project under this study was designed to understand the microbial interactions and emergence of predominance within biofilm constitutive …

The Role And Molecular Mechanisms Of Wss1 In Preserving Genomic Stability, Daniel Kwesi Sam

Electronic Theses and Dissertations

Cells are constantly under threat from both exogenous and endogenous sources of DNA damage. Eukaryotic organisms, however, possess conserved mechanisms that accurately and faithfully respond to DNA damage. The inability to effectively remove DNA lesions can lead to an accumulation of mutations which can compromise cellular viability. The DNA damage response is conserved from bacteria to eukaryotic organisms and have been well characterized, however, how covalently crosslinked proteins are removed from DNA remains enigmatic This thesis provides genetic and biochemical evidence implicating Wss1, a yeast metalloprotease in genome maintenance. We have identified SUMOylation to be an important signal that mediates …

Understanding The Effect Of Single- And Twin-Screw Extrusion Processing Parameters On Physical And Physico-Chemical Properties Of Sprouted Quinoa And Sprouted Proso Millets, Gabriela John Swamy

Electronic Theses and Dissertations

According to Mordor Intelligence Research, the new compositional research and moisture content extrusion process are helping the growth of the plant protein market. The demand for plant proteins is growing at a fast rate, owing to change in lifestyle, lack of balanced dietary intake, and improved research and development in order to develop new kinds of plant-protein enriched products. It is necessary to identify the right plant protein sources and choose the right processing methodology to create highly digestible foods which can be consumed by infants and elderly as well. In addition, it is important to support local farmers and …

A Series-Elastic Robot For Back-Pain Rehabilitation, Elhussein Shata

Electronic Theses and Dissertations

Robotics research has been broadly expanding into various fields during the past decades. It is widely spread and best known for solving many technical necessities in different fields. With the rise of the industrial revolution, it upgraded many factories to use industrial robots to prevent the human operator from dangerous and hazardous tasks. The rapid development of application fields and their complexity have inspired researchers in the robotics community to find innovative solutions to meet the new desired requirements of the field. Currently, the creation of new needs outside the traditional industrial robots are demanding robots to attend to the …

Impact Of Postweaning Management Strategies On Beef Carcass Characteristics And Meat Quality, Christina Elaine Bakker

Electronic Theses and Dissertations

The overall objective of this dissertation was to determine the impact of selected post weaning management strategies on beef carcass characteristics and meat quality. The specific objectives were 1) determine the effect of a rumen-protected long chain fatty acids fed during finishing on live performance, serum fatty acid profile, carcass traits, and predicted carcass composition, and 2) determine effects of feeding brassica-based cover crops to cattle during backgrounding on live animal performance, carcass characteristics, tenderness, juiciness, and flavor of strip steaks, and case life of ground beef and strip steaks. Results from both objectives indicate post weaning management strategies do …

Cold-Water Prey Fish Monitoring And Ecology In Lake Oahe, Nicholas B. Kludt

Electronic Theses and Dissertations

In Lake Oahe, a large mainstem Missouri river reservoir that spans 375 river kilometers in central North and South Dakota, naturalized Rainbow Smelt are the primary forage species for a regionally important Walleye Sander vitreus fishery. Walleye condition in Lake Oahe is highly correlated to Rainbow Smelt relative abundance through time, the latter species being typified by erratic abundance trends since accidental introduction in 1971. Cisco Coregonus artedii were introduced in the early 1990s to augment the cold-water prey base. We evaluated Lake Oahe cold-water prey base monitoring and dynamics through: 1.) a comparison of two hydroacoustic survey designs, 2.) …

Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc

Electronic Theses and Dissertations

This research aimed to understand the evolutionary mechanism of seed dormancy and flowering time and to provide basic knowledge for the manipulation of germination features in crop breeding. Flowering time and seed dormancy are two adaptive traits for flowering plants. Seed dormancy (SD) helps regulate the timing of germination to promote the survival of seed-bearing plants in adverse environments. Cereal crops and weedy/wild relatives, such as Asian cultivated rice and weedy rice (Oryza sativa L.), have weak and strong seed dormancy, respectively. Thus, weedy and cultivated rice has been used as a model system to investigate mechanisms of seed dormancy …

Understanding How Agricultural Intensification Impacts Ring-Necked Pheasant Distribution And Survival In Eastern South Dakota, Hilary R. Kauth

Electronic Theses and Dissertations

Historically, pheasants (Phasianus colchicus) have thrived in South Dakota in conjunction with successful land retirement programs or early farming practices through the 1950s, which created interspersions of agriculture and native landscapes that were ideal for pheasants. Recently, the Prairie Pothole ecosystem has undergone rapid agroeconomic expansion, effectively reducing ideal interspersions of native prairie and cropland into agriculturally homogeneous landscapes. Indices of pheasant abundance have suggested persistent population declines since 2008, raising concerns regarding landscape suitability. Our goal was to understand how agriculture intensification impacts pheasant ecology. The objectives were to: 1) estimate overwinter hen probability of survival, resource …