Digital Commons Network^™

Depressive Symptoms And Survival Among Lung Cancer Patients: Biomarkers And Potential Benefits Of An Ipod- And Mindfulness-Based Intervention., Chelsea J. Siwik

Electronic Theses and Dissertations

Lung cancer patients experience high levels of distress, which commonly manifests as depressive symptoms. Importantly, depressive symptoms have demonstrated prognostic significance in cancer contexts, although the biological pathways by which depressive symptoms lead to poorer survival remain unclear and warrant greater attention. In addition to understanding the biological pathways by which depressive symptoms accelerate disease, identification of efficacious and effective psychological treatments for depressive symptoms are needed to improve both quality and quantity of life for cancer patients. Interventions that reduce depressive symptoms and improve downstream clinical outcomes are certainty needed; however, because many cancer patients face considerable burden, interventions …

Electrochemical Reduction Of Carbon Dioxide And Carbon Monoxide For The Production Of Green Fuels And Chemicals., Jacob M. Strain

Electronic Theses and Dissertations

It has become apparent that closing the carbon cycle on this planet in order to mitigate disastrous consequences of runaway global warming has become one of the most pressing issues of our civilization. One of the ways we need to accomplish this goal is by finding news methods to generate fuels that will be carbon neutral. Renewable fuels and green chemicals will be a major component of closing the carbon cycle and restoring our planet’s ecosystem into a sense of balance. A method that can help achieve this goal is the reduction of CO₂. If CO₂ can …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …

Computational Modeling Of Photolysis And Catalysis Reactions In Vitamin B12-Dependent Enzymes., Abdullah Al Mamun

Electronic Theses and Dissertations

Vitamin B₁₂ is a complex organometallic molecule, the derivatives of which such as adenosylcobalamin (AdoCbl) and methylcobalamin (CH₃Cbl) act as a cofactor in numerous enzymatic reactions. These two biologically active cofactors contain a unique organometallic Co-C σ bond. Important feature of this Co-C bond is that it can be activated by both thermally and photolytically inside the enzymatic environment as well as in the solution. In the case of enzymatic reactions where AdoCbl molecule act as a cofactor, the cleavage of the Co-C bond constitutes the key catalytic step. The most intriguing features of this cleavage is …

Oral Health-Related Quality Of Life In Us Adults With Type 2 Diabetes., Giang Truong Vu

Electronic Theses and Dissertations

This dissertation focused on the relationship between type 2 diabetes (T2DM) and oral health-related quality of life (OHRQoL) and the factors associated with this relationship in the US. The objectives of the dissertation research were a three-part process: (1) to understand the relationships of T2DM with poor OHRQoL, clarify risk and preventive factors that affect this association, and identify research gaps in the literature, (2) to analyze risk factors associated with poor OHRQoL in US adults with T2DM using a nationally representative random probability cluster sample, and (3) to apply structural equation modeling (SEM) to a simplified Andersen’s Behavioral Model …

Transient Display Of Chimeric Proteins On Biological Surfaces As An Effective Strategy For Modulations Of Innate And Adaptive Immune Responses., Pradeep Shrestha

Electronic Theses and Dissertations

The major premise of this dissertation was to transiently display novel immunological ligands on biological membranes as a localized means of modulating innate and adaptive immune responses with applications to bone marrow and pancreatic islet transplantation. In Chapter two, we engineered donor allogeneic bone marrow cells to transiently display a novel form of FasL, SA-FasL, to efficiently purge out alloreactive donor T cells to prevent acute GVHD. In Chapter three, we engineered pancreatic islets with a novel form of CD47, SA-CD47, to modulate instant blood mediated inflammatory reaction (IBMIR) to prevent immediate islet graft loss following intraportal transplantation. GVHD is …

Predictors Of Binge Drinking Among Military Veterans: A General Strain Theory Approach., Emily Ann Hayden

Electronic Theses and Dissertations

This dissertation is theoretically informed by General Strain Theory (GST) (Agnew 1992, 2002), and examines binge drinking within a military veteran population. It begins with an assessment on reform and focuses on shaping knowledge around the issue of binge drinking by military veterans. The current study uses Agnew’s GST to argue for an understanding of how strain and stress can affect individuals who have served in the military and acknowledges how this strain can lead to negative coping mechanisms such as binge drinking. The latter part of the dissertation argues that understanding Agnew’s GST (1992, 2002) with a military population …

Novel Bayesian Methodology For The Analysis Of Single-Cell Rna Sequencing Data., Michael Sekula

Electronic Theses and Dissertations

With single-cell RNA sequencing (scRNA-seq) technology, researchers are able to gain a better understanding of health and disease through the analysis of gene expression data at the cellular-level; however, scRNA-seq data tend to have high proportions of zero values, increased cell-to-cell variability, and overdispersion due to abnormally large expression counts, which create new statistical problems that need to be addressed. This dissertation includes three research projects that propose Bayesian methodology suitable for scRNA-seq analysis. In the first project, a hurdle model for identifying differentially expressed genes across cell types in scRNA-seq data is presented. This model incorporates a correlated random …

Sulforaphane Protects Against Ethanol-Induced Apoptosis And Teratogenesis Through Epigenetic Modulation Of Anti-Apoptotic Genes., Yihong Li

Electronic Theses and Dissertations

Background. Ethanol-induced excessive apoptosis in neural crest cells (NCCs), a multipotent progenitor cell population, is one of the major mechanisms underlying the pathogenesis of Fetal Alcohol Spectrum Disorders (FASD). However, the molecular mechanisms underlying FASD that results from maternal alcohol exposure during pregnancy are poorly understood. The overall goals of this study are to examine the mechanisms by which ethanol induces apoptosis and malformations in vitro and in vivo, and to develop a nutritional-based approach by using SFN and SFN-rich BSE to prevent FASD through epigenetic modulation. Results. This study demonstrates that ethanol exposure resulted in a significant increase …

The Role Of Slc7a11 In Controlling Extracellular And Intracellular Redox Environments Of Lung Fibroblasts - Potential Targets For Intervention In Aging And Idiopathic Pulmonary Fibrosis., Yuxuan Zheng

Electronic Theses and Dissertations

Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease characterized by extracellular matrix deposition by fibroblasts. Aging and oxidative stress increase the susceptibility to IPF. Redox couples, cysteine/cystine (Cys/CySS) and glutathione/glutathione disulfide (GSH/GSSG), and their redox potentials (E_h) quantify oxidative stress. Fibroblasts from old mice maintain more oxidized extracellular E_h(Cys/CySS) than young mice. Microarray shows down-regulation of Slc7a11 potentially mediates this age-related oxidation. Slc7a11 is the key component of system X_c^-, an antiporter that imports CySS and exports glutamate. The first aim of this dissertation is to investigate the mechanistic link between Slc7a11 …

The Cardiovascular Effects Of Tobacco Product-Derived Aldehydes: Contribution Of Transient Receptor Potential Ankyrin-1., Lexiao Jin

Electronic Theses and Dissertations

Cardiovascular diseases (CVD) are the leading cause of death in most countries and are significant health and financial burden worldwide. Smoking contributes to more than 480,000 premature deaths annually -- accounting for one of every five deaths in the United States each year. Numerous studies have shown that smoking increases the risk of factors including endothelial dysfunction, thrombosis, and atherosclerosis, which may lead to an incidence of myocardial infarction, coronary artery disease, and stroke. Electronic cigarettes (e-cigarettes) are a new kind of tobacco product, and some studies show that e-cigarettes also could induce CVD since both e-cigarettes and conventional tobacco …

Addition Of Fermentation Experiment To Unit Operations Laboratory, Jeremy T. Rone

Electronic Theses and Dissertations

With the growth of the distillery and brewery industries and the potential jobs created for chemical engineers, it is imperative to equip the chemical engineering graduates from the University of Louisville with knowledge of bioreaction kinetics and experience in performing calculations to solve for concentration profiles and reaction rates. To accomplish this, it is recommended to add a fermentation experiment to the Unit Operations Laboratory II course.

Four groups of students from the Spring 2020 Unit Operations Laboratory II course performed the fermentation experiment. They were able to successfully complete all lab requirements as well as analyze the results and …

Determination Of Selenite And Selenate In Wet Flue Gas Desulfurization Process Water Through Ic-Icp-Ms And Hg-Icp-Aes, Samuel Anthony Kelty

Electronic Theses and Dissertations

During coal-fired electric power production, coal bound elemental selenium is released into the gas phase and oxidized to selenite and selenate in the wet flue gas desulfurization (WFGD) unit. Selenite and selenate solubilize in the WFGD process water. A process water system at a coal fired power plant contributes to environmental regulation compliance by removing the selenium species from WFGD process water. A selenium speciation method was developed for the purpose of better monitoring the selenium species present in the “treated” process water and to aid the process water system in meeting compliance with the Environmental Protection Agency’s Effluent Limitation …

Error-Related Negativity And Feedback-Related Negativity On A Reinforcement Learning Task, Elizabeth Ridley

Electronic Theses and Dissertations

Event-related potentials play a significant role in error processing and attentional processes. Specifically, event-related negativity (ERN), feedback-related negativity (FRN), and the P300 are related to performance monitoring. The current study examined these components in relation to subjective probability, or confidence, regarding response accuracy on a complicated learning task. Results indicated that confidence ratings were not associated with any changes in ERN, FRN, or P300 amplitude. P300 amplitude did not vary according to participants’ subjective probabilities. ERN amplitude and FRN amplitude did not change throughout the task as participants learned. Future studies should consider the relationship between ERN and FRN using …

"No One's Gonna Say That At Church:" Women's Experiences With Infertility In Christian Faith Communities, Donna Paulsen

Electronic Theses and Dissertations

This study explores women’s experiences with infertility in Christian faith communities. Drawing from nine one-on-one interviews, the author argues that the presence of particular religious ideologies, social interactions, and rituals within faith communities contributed to the stigmatization and marginalization of study participants. Employing Muted Group Theory, the author uncovers the communicative strategies infertile women employ to resist these oppressive practices. A qualitative analysis of participants’ narratives presents two principal categories, containing a total of four findings relating to the harmful beliefs and practices of these women’s faith communities. The author argues that the veneration of motherhood and children suggests that …

“A Lot Of Prayer, And Some Wine In-Between”: Applying The Relational Turbulence Model To The Stepparent-Biological Parent Marriage., Paul Taylor

Electronic Theses and Dissertations

The present study utilizes the relational turbulence model (RTM) to illuminate stepparent experiences of relational uncertainty and partner interference within the context of the stepparent-biological parent marriage. In-depth, semi-structured interviews with 6 stepparents revealed four primary themes pertaining to how stepparents experienced relational uncertainty: (a) enactment of parenting, (b) competing expectations, (c) shifts in attitude and behavior by spouse, and (d) differences between marriage partners. Interference from partners was experienced by stepparents in relation to the enactment of parenting and the maintaining of the marriage as an intimate relationship.

Computational Quantum Study Of Intermediates Formed During The Partial Oxidation Of Melatonin, Oladun Oladiran

Electronic Theses and Dissertations

Melatonin is a neurohormone produced by the pineal gland in the brain. It functions as an antioxidant to scavenge free radicals. Free radicals are reactive species; they often oxidize the cells leading to oxidative stress which may lead to severe health complications. Reaction of melatonin with free radicals is known to be stepwise, as such the stability of the intermediates can be examined. Thus, the possibility of using melatonin as an in vivo spin trap can be determined. Spin traps allow characterization of unstable radical species using electron spin resonance spectroscopy. In this research, ab initio quantum chemistry techniques were …

Trends In Advertising: How The Rise In Artificial Intelligence May Influence The Field Of Content Strategy, Joel Eaton

Electronic Theses and Dissertations

Whereas prior research on artificial intelligence has dealt with automation in fields like medicine, engineering, and computer science, this research study seeks to answer the question, “To what extent can AI be creative in the context of content strategy?” To answer this, this study employs content analysis using 16 online news and blog articles from primarily marketing organizations to identify and explain key variables surrounding the relationship between the computer and the creative professional. This study has found that the core belief that AI will play the future role of creative assistant in the context of content strategy is shared …

Teacher Tenure In K-12 Public Education: A Study Of Tennessee Tenure Law, Lucas Winstead

Electronic Theses and Dissertations

The purpose of this study is to examine the Tennessee tenure law by comparing the overall level of effectiveness of teachers who received tenure prior to receiving tenure and the overall level of effectiveness of teachers after receiving tenure. The population of this study includes teachers from districts in the Mid Cumberland region in Tennessee who received tenure after 2012. The major finding of this study was the effectiveness of teachers who received tenure under the current tenure law in Tennessee did not significantly change for up to two years after they received tenure. The lone exception was high school …

Efficient Conditional Inactivation Of Rdh10 Reveals Important Role Of Retinoic Acid During Lung Branching Morphogenesis., Nhut Quang Huy Tran

Electronic Theses and Dissertations

Birth defects are complicated pathological processes. Many birth defects can be traced back to disrupted regulation of molecular signaling pathways during embryogenesis. One of the most important gene regulatory factors during embryo development is retinoic acid (RA). However, studying the specific roles of RA in each organ during embryogenesis in vivo is challenging. The Sandell laboratory has a conditional mutant mouse model that can potentially be used for studying the role of RA during embryogenesis by inducing stage-specific RA deficiency. This model allows retinol dehydrogenase 10 (Rdh10), a gene required for RA production, to be inactivated at a …

Using Self-Monitoring And Positive Reinforcement To Increase On-Task Behavior And Independence., Jon Scott

Electronic Theses and Dissertations

Off-task behavior is a major challenge. Various interventions have addressed this problem. Self-monitoring interventions are very effective, including the MotivAider, a self-timer that silently signals the student to observe his/her own Academic Engagement Time (AET). Studies of the MotivAider have reported increased AET., (Legge, DeBar, & Alber-Morgan, 2010; Morrison, McDougal, Black, & King-Sears, 2014) systematically faded the MotivAider to sustain increased AET. The present study replicated and extended this research using a response-dependent fading (Fox, Shores, Lindeman, & Strain, 1984) of the MotivAider to sustain the observe AET of a 6^th grade student with Learning Disabilities. A single …

Alcohol Consumption In A Preclinical Model Of Schizophrenia, Liza Hernandez

Electronic Theses and Dissertations

Schizophrenia is a debilitating psychiatric disorder that affects approximately 1% of the global population. Schizophrenia is highly comorbid with other psychiatric disorders such as Alcohol Use Disorder (AUD) with a prevalence rate of 27% - 65%, which is significantly higher than AUD exhibited by the general population (6%). Research indicates that a higher rate of AUD in individuals suffering from schizophrenia may be related to the common neuronal pathways that underlie the expression of both disorders. The present study will determine whether the neonatal quinpirole (NQ) rodent model of schizophrenia will approximate the human condition and exhibit increased EtOH consumption. …

Changes In Countermovement Jump And Sprint During A Congested Match Schedule In Female Youth Soccer Players, Joanne Spalding

Electronic Theses and Dissertations

Youth soccer players are required to play multiple matches within a short period of time. Participation in congested match schedules like youth soccer tournaments can result in fatigue and a decline in physical performance. The purpose of this study was to examine changes in countermovement jump height variables and sprint time to determine if physical performance was altered. Fourteen youth female soccer players performed countermovement jump testing pre, post-match, and post-tournament, and sprint testing pre and post-tournament. Jump height differed significantly (p

Supplemental Instruction In First-Year Chemistry Courses: Efficacy And Gender Balance, Deidre R. Johnson

Electronic Theses and Dissertations

Increasing student success and retention rates are top goals for many higher education institutions. Supplemental Instruction (SI) is a widely used academic support program designed to increase students’ academic performance and provide students the learning skills they need to persist to graduation. Unfortunately, a lack of time, personnel, and resources often prevent in-depth, meaningful analysis on the effectiveness of SI programs. This study examined the relationship of attending SI sessions for first-year chemistry courses to student grade outcomes and retention rates for attendees. The gender of SI leaders and SI participants was also assessed to determine if leader and participant …

Chronoecology Of A Cave-Dwelling Orb-Weaver Spider, Meta Ovalis (Araneae: Tetragnathidae), Rebecca Steele

Electronic Theses and Dissertations

Circadian clocks enable coordination of essential biological and metabolic processes in relation to the 24-hour light cycle. However, there are many habitats that are not subject to this light cycle, such as the deep sea, arctic regions, and cave systems. This study analyzes the circadian pattern of isolated populations of a subterranean spider, Meta ovalis from two Tennessee caves and five Indiana caves. Locomotor activity was recorded with TriKinetics LAM50 Locomotor Activity Monitor under a 12-hour light (L), 12-hour dark (D) (LD 12:12) cycle preceding total darkness (DD). Significant differences were found within and among populations found in Tennessee cave …

Religion, Health, And The Spiritual But Not Religious, Alexander L. Smith

Electronic Theses and Dissertations

Previous literature shows an overwhelmingly positive relationship between religiosity and health. Researchers theorize that service attendance, social capital, and group identity verification mediates this positive relationship. There is an upward trend in secularity in the U.S. with more individuals describing themselves as spiritual but not religious (SBNR).Current research does not clearly depict who comprises the SBNR or how varying degrees of secularity fit into the relationship between religion and health. Using data from the GSS, this study examines basic sociodemographic characteristics of the SBNR and compares the SBNR to other religious and secular groups on various health measures. This study …

When Beauty Is More Than Skin Deep: A Content Analysis Of Popular Beauty Youtubers’ Video Strategies, Kristen Jones

Electronic Theses and Dissertations

The beauty community on YouTube is a popular outlet for influencer generated video content. Beauty YouTubers provide their viewers with makeup-related videos from tutorials to the latest trends on the platform. As a result, the influencers have gained high subscriber counts that generate revenue through advertisements and brand partnerships. The influencers create relationships with their subscribers that lead to loyalty in the form of video views and merchandise purchasing. This study provides a content analysis of common characteristics within 10 popular beauty YouTubers’ videos examining the strategies used by the influencers. The top five videos from each beauty YouTuber were …

Investigation Of Multiple Imputation Methods For Categorical Variables, Samantha Miranda

Electronic Theses and Dissertations

We compare different multiple imputation methods for categorical variables using the MICE package in R. We take a complete data set and remove different levels of missingness and evaluate the imputation methods for each level of missingness. Logistic regression imputation and linear discriminant analysis (LDA) are used for binary variables. Multinomial logit imputation and LDA are used for nominal variables while ordered logit imputation and LDA are used for ordinal variables. After imputation, the regression coefficients, percent deviation index (PDI) values, and relative frequency tables were found for each imputed data set for each level of missingness and compared to …

Understanding The Implications Of Anandamide, An Endocannabinoid In An Early Land Plant, Physcomitrella Patens, Md Imdadul Haq

Electronic Theses and Dissertations

Endocannabinoid signaling is well studied in mammals and known to be involved in numerous pathological and physiological processes. Fatty acid amide hydrolase (FAAH) terminates endocannabinoid signaling in mammals. In Physcomitrella patens, we identified nine orthologs of FAAH (PpFAAH1 to PpFAAH9) with the characteristic catalytic triad and amidase signature sequence. Kinetics of PpFAAH1 showed specificity towards anandamide (AEA) at 37°C and pH 8.0. Further biophysical and bioinformatic analyses revealed that, structurally, PpFAAH1 to PpFAAH4 were closely associated to the plant FAAH whereas PpFAAH6 to PpFAAH9 were more closely associated to the animal FAAH. A substrate entry gate or ‘dynamic paddle’ …

Storm Sampling To Assess Inclement Weather Impacts On Water Quality In A Karst Watershed: Sinking Creek, Watauga Watershed, East Tennessee, Porcha Mccurdy

Electronic Theses and Dissertations

Escherichia coli changes in Sinking Creek, an impaired water body in the Watauga watershed of northeast Tennessee, were assessed during storm events using water samples collected with ISCO automated samplers during eight storms at two locations. Turbidity and electrical conductivity (EC) data loggers were deployed in the creek, and dissolved oxygen (DO) was measured in situ to test the stream’s water quality and reaction to inclement weather. Cotton fabric was deployed at both locations and sent to an external lab to test for the presence of Optical Brighteners (OB), which are indicators of residential wastewater. E. coli and turbidity at …