Digital Commons Network^™

Modeling Disorder In Proteins Yields Insights Into The Evolution Of Stability And Function, Jonathan Huihui

Electronic Theses and Dissertations

The central dogma of molecular biology dictates that a DNA sequence codes for an RNA sequence, which in turn codes for a sequence of amino acids that comprises a protein. Proteins are responsible with performing myriad functions within living organisms and most proteins require a folded structure in order to perform their function. The protein's structure is the direct link from sequence to function. This is known as the sequence - structure - function paradigm. However, this does not mean that the unfolded state is unimportant. In order to properly model the stability of the folded state, one needs to …

The Role Of Dehumanization In The Nazi Era In Activating The Death Drive Resulting In Genocide, Stewart Gabel

Electronic Theses and Dissertations

Dehumanization can be defined in part as a process by which a powerful individual or group (the victimizers) actively denies or withdraws a second individual’s or group’s (the victim’s) sense of human worth or personal value. Dehumanization is an especially virulent form of denigration of the Other and is known to have harmful psychological consequences on victims.

The thesis of this dissertation is: Dehumanization, applied in an increasingly severe manner to demean, subjugate and control Jews in Nazi dominated territories during the Nazi era (1933-1945), activated a “death instinct/drive” (Freud 1920; 1923/1960; 1930) that was used to resolve an extreme …

Intellectual Property Rights In The Seed Industry: Barriers To Sustainable Agriculture, Elena A. Filatova

Electronic Theses and Dissertations

The dynamics of the dominant industrial agriculture system restrict the seed industry’s innovative landscape, leading to significant negative consequences including an exacerbation of environmental risks which threaten global food security. This thesis explores how exclusionary intellectual property rights (IPRs) in the context of the seed industry constrict innovation, evolutionary pathways, and opportunities for the implementation of sustainable agriculture methods. To overcome these barriers, the application of an open source framework to seed innovation, specifically through the platform of the Open Source Seed Initiative, is evaluated as a tool for enhancing innovative capacities in seed development while broadening the accessibility and …

Exploring The Relationship Between Adverse Childhood Experiences And Paternal Warmth: Does Racial Identity Moderate This Relationship And Does Depression, Anxiety, And Physical Health Mediate This Relationship?, Stephanie Rachel Speer

Electronic Theses and Dissertations

Background: Childhood trauma has a direct impact on parenting. Parents with a history of adverse childhood experiences are more likely to struggle with mental illness in adulthood and have children at an augmented risk for behavioral health issues. However, most of this work has focused on mothers, and few studies have explored how adverse childhood experiences influence paternal warmth and father involvement. Research on fathering has identified personal (e.g., age, race, income, parental stress, depression, and anxiety) and social (e.g., residential and relationship status, co-parenting) predictors of paternal warmth and father involvement. While poor physical health may influence parenting behaviors, …

Twentieth Century Pandemic Narratives And Mental Health Discourse, Kristy R. Barraza

Electronic Theses and Dissertations

This paper utilizes René Girard’s theories concerning plague literature to examine twentieth century pandemic novels’ engagement with mental health discourses surrounding anxiety and melancholia. Girard argues that plague literature consists of four main elements: contamination, dissipation of differences, doubles, and sacrifice; he also argues that the plague represents violence. In 1918, a plague of influenza killed more people in the United States than all the wars from the twentieth century combined. William Maxwell’s They Came Like Swallows and Katherine Anne Porter’s Pale Horse, Pale Rider depict the trauma caused by the 1918 pandemic; Maxwell shows how the 1918 influenza disrupted …

The Place And Role Of Pharmacy Benefit Management (Pbm) Companies In Us Healthcare System, George Mattis Ii

Electronic Theses and Dissertations

On average, prescription drugs cost US consumers more than any other developed country in the world. US drug makers claim that intermediaries in the prescription drug supply chain, companies called pharmacy benefit managers (PBM), are forcing them to increase their drug prices. PBMs counter that the discounts they receive from drug makers are channeled to insurers. This thesis will examine the role that PBMs play in the prescription drug supply chain and determine what effect they have on drug prices. This thesis will utilize a comparative static model, Structure, Conduct, Performance (SCP) framework to analyze the pharmaceutical manufacturing and the …

Anger Bias In The Evaluation Of Crowds, Diana Mihalache

Electronic Theses and Dissertations

Although people are good at classifying emotions, they also make mistakes. These errors tend to be negatively biased and potentially serve a protective function. Research on biases in emotion perception has largely focused on perception of individual faces and little is known about biases in evaluations of crowds. In the first investigation, I conducted six experiments, evaluating anger bias—a tendency to judge facial expressions as angry—in the context of single faces and emotionally homogenous crowds. I found that observers were biased to classify faces as angry, especially when evaluating crowds. This amplified bias emerged in the context of perceptual uncertainty …

The Social Determinants Of Diabetes And Coronary Heart Disease In South Asian American Immigrants, Mishal Ayaz

Electronic Theses and Dissertations

An astounding 20% of South Asian Americans have diabetes (Matthews and Zachariah 2008). Conventional risk factors for coronary heart disease includes: age older than 65, sedentary lifestyle, cigarette smoking, hypertension, elevated low-density lipoprotein (LDL), cholesterol, and type 2 diabetes, all factors beyond health care (italicized for emphasis) (Mathews and Zachariah 2008). But conventional risk factors alone are not sufficient to predict the alarmingly high rates of coronary heart disease (“CHD”) for South Asian Americans. In fact, the only conventional risk factor more prevalent in this community than others is diabetes. So, the question remains, what factors are contributing to the …

The Ecological Resilience Of A Tropical Montane Pine Forest To Droughts, Fire, And Hurricane Disturbance, Daniel Erland Brugger Swann

Electronic Theses and Dissertations

Owing to the geographic isolation and unique climatic conditions which occur in tropical montane forests (TMFs), these systems are highly sensitive to changes in both climate and disturbance. Therefore, as ongoing climate change alters disturbance regimes – potentially facilitating multiple disturbance interactions much greater than that of isolated disturbances – TMFs may be shifted to alternate stable states. However, study of TMF resilience is essentially unknown given the lack of long-term studies in these systems. We address the resiliency of the tropical montane pine forests of the Cordillera Central, Dominican Republic using long-term climate records, remeasured vegetation data, and reconstructed …

“But I’M Not Racist”: How Implicit Racial Bias, Feedback And Racial Affective States Impact Clinical Judgment In Mental Health Treatment, Dhriti Tiwari

Electronic Theses and Dissertations

Implicit bias has gathered research interest in healthcare, yet remains less directly examined in the mental health field (Merino et al., 2018). Mental health providers can continue to be influenced by implicit bias despite higher ratings of cultural competence (Boysen, 2010). The purpose of this study was to supplement the limited research examining the impact of implicit bias on the clinical judgment process. The study aimed to examine whether: 1) implicit race bias scores were related to diagnostic impressions, 2) feedback about implicit bias was related to diagnostic impressions, and 3) racial affect mediated the relationship between receiving feedback and …

Coming Together Over Table: The Role Of Food In Georgian Conflict Resolution Practices, Raisa Wells

Electronic Theses and Dissertations

Conflict resolution efforts seek to de-escalate conflict dynamics to bring conciliation and/or reconciliation to a conflict. One strategy to de-escalate a conflict is to use food during conflict resolution efforts. So, what specifically does consuming food and beverage do to break down conflict escalation cycles? Food-sharing brings several aspects to conflict that the literature suggests address how and why conflict escalates. This paper focuses on three prevalent aspects: how food-sharing signals vulnerability and trust building, perceived commonality, and a change in the conflict from competition to cooperation by providing new norms, changing the tone, and shifting frames. Because of the …

“It's Like A Phantom Limb. It Feels Like It’S There. It's Supposed To Be There, But It's Not.”: Birthmothers’ Metaphors Of Ambiguous Loss, Melodee Lynn Sova

Electronic Theses and Dissertations

Birthmothers are a population with unique experiences and challenges. Among those challenges is the difficulty understanding and managing a loss for which they do not have a readily available coping strategy. As a birthmother, the author of this dissertation was uniquely situated to connecting with, and understanding the ways in which, birthmothers expressed their narratives. The purpose of this dissertation is to explore the ways in which birthmothers make sense of their experiences with ambiguous loss by examining the metaphors they employ when narrating their adoption story. The use of metaphor in the birthmother narrative was especially important because metaphor …

Notch Regulates Vascular Collagen Iv Basement Membrane Through Modulation Of Lysyl Hydroxylase 3 Trafficking, Stephen J.B. Gross

Electronic Theses and Dissertations

Collagen type IV (Col IV) is a basement membrane protein associated with early blood vessel morphogenesis and is essential for blood vessel stability. Defects in vascular Col IV deposition are the basis of heritable disorders, such as small vessel disease, marked by cerebral hemorrhage and drastically shorten lifespan. To date, little is known about how endothelial cells regulate the intracellular transport and selective secretion of Col IV in response to angiogenic cues, leaving a void in our understanding of this process. Our aim was to identify trafficking pathways that regulate Col IV deposition during angiogenic blood vessel development. We have …

Understanding Patterns And Functional Impacts Of An Invasive Tree And Its Biological Control In A Riparian System, Annie L. Henry

Electronic Theses and Dissertations

Invasive species have become an inextricable part of the landscape, particularly in riparian plant communities, and removal is often a key component of restoration programs. Biological control (biocontrol) is a method of removal that is often both efficient and effective. However, the impact of biocontrol on target species and indirect effects from invasive species removal can be hard to predict. While monitoring the impact of invasive species removal usually involves some species-based assessment such as changes in diversity, historically dominant species or native species, these strategies do not typically provide insight into the mechanisms underlying plant community response to removal. …

The Experiences And Mental Health Impact Of Islamophobia On Muslim Americans Following The 2016 U.S. Presidential Election: A Hermeneutic Phenomenological Study, Hadeel Ali

Electronic Theses and Dissertations

The purpose of the current hermeneutic phenomenological qualitative study was to understand the phenomenon of Muslim Americans’ lived experiences of Islamophobia prior to and two years into Donald Trump’s presidential administration. 14 participants from multiple regions in the United States completed a semi-structured interview via telephone. The data analysis revealed seven major themes: 1) Muslim Americans experience different dimensions of Islamophobia, 2) Muslim Americans experience various forms of Islamophobia, 3) Variables that impact the prevalence of Islamophobia, 4) Islamophobia impacts various areas of Muslim Americans’ lives, 5) Muslim Americans may react differently to experiences of Islamophobia, 6) Islamophobia impacts the …

Stories Of Return: A Collection Of Repatriation Narratives, Lydia Degn-Sutton

Electronic Theses and Dissertations

This thesis examines the museological phenomena of repatriation beyond NAGPRA and the incorporation of Indigenous curatorial methods into museum collections practices. The project explores repatriation and collections caretaking practices at ten settler institutions through narratives of experience collected from museum staff. The findings of this research suggest that repatriation beyond NAGPRA and the Indigenization of collections care are situated processes that should be understood contextually and historically. This thesis argues that, in some cases, repatriation beyond NAGPRA and the integration of Indigenous perspectives, practices, and protocols into museum collections stewardship demonstrates a willingness by institutions to go beyond the minimum …

Maternal Hpa Axis Function During Parenting Is Associated With Reduced Brain Activation To Infant Cry And More Intrusive Parenting Behavior, Andrew Erhart

Electronic Theses and Dissertations

Previous research indicated that maternal cortisol function and maternal brain response to infant stimuli are separately related to differences in parenting behavior. Evidence from animal models have demonstrated that chronically high cortisol concentration alters brain structure and function, suggesting that studying these two mechanisms together may further improve understanding of parental behavior in human mothers. First time mothers of infants aged 1-7 months old (M age = 3 months) were recruited to participate. Mother’s cortisol concentration was measured during a naturalistic interaction with their infant and their behavior was coded for maternal sensitivity and nonintrusiveness. In a separate session using …

Design Of Lower Legs Of Mithra, A High-Performance Backdrivable Humanoid Robot, Drake Taylor

Electronic Theses and Dissertations

This thesis presents the design of the knee and ankle of Mithra, a new humanoid robot that aims to be an energy-efficient and highly agile machine. Mithra makes use of new optimization metrics for legged robots to develop a system capable of mimicking human movement. A series of low-impedance, high-torque actuator systems were developed with the goal of creating lightweight, powerful, and robust motion. The structure of Mithra's legs mimics the human structure in leg segment length and weight proportions. Detailed design and analysis were conducted in order to allow Mithra to be a robust and maintainable system. Mithra will …

Development Of Endoplasmic Reticulum Targeted Probes And Red Fluorescent Probes For Detecting Zinc, Drew Maslar

Electronic Theses and Dissertations

Zinc (Zn²⁺) is the second most abundant transition metal in the body and is important in various biological functions. Fluorescent sensors based on circularly permuted fluorescent proteins (cpFPs) have been previously made to detect labile, or unbound, Zn²⁺ within the cytoplasm of cells. These sensors have proven invaluable for studying Zn²⁺, however, these sensors are limited to their use in the cytoplasm and by the fact that only green cpFP have been utilized to create fluorescent Zn²⁺ sensors. In this thesis, we use a combination of peptide targeting sequences, site-directed mutagenesis, and rational design …

Validation Of A Deployable Proteomic Assay For The Serological Screening Of Sexual Assault Samples, Catherine O'Sullivan Brown

Electronic Theses and Dissertations

Protein mass spectrometry (MS) has emerged as a technique to supplant traditional serological tests for body fluid identification. It was hypothesized that proteomic techniques would surpass the sensitivity and specificity of traditional serological techniques. An automated workflow coupled with protein MS has been developed for the confirmatory identification of five biological fluids. A developmental validation was completed, assessing parameters such as reproducibility, sensitivity, ion suppression, and limit of detection. Implementation was determined through tandem sample processing by MS, traditional serological tests, and standard DNA profiling methods. The MS approach offered superior detection limits while also providing true confirmatory results, producing …

Preconception Stress, Affect-Biased Attention, And Negative Affectivity: Assessing Pathways And Early Markers Of Psychopathological Risk In Infancy And Childhood, Danielle A. Swales

Electronic Theses and Dissertations

The developmental origins of mental health likely begin early in life and perhaps even prior to conception. Research is needed to elucidate pathways of risk and resilience in the development of psychopathology. The goal of the current dissertation was to explore how both preconception and postnatal experiences influence negative affectivity, a robust and transdiagnostic risk factor for later psychiatric symptoms. The present dissertation accomplished this goal by completing two independent studies, each of which are presented in the format of standalone journal articles. Study one focused on evaluating how preconception experiences, specifically maternal symptoms of post-traumatic stress disorder (PTSD), impact …

Mechanisms Of Sensory Adaptation In The Primate Visual System, Boris Isaac Peñaloza Rojas

Electronic Theses and Dissertations

Under ecological conditions, the luminance impinging on the retina varies within a dynamic range of 220 dB. Stimulus contrast can also vary drastically within a scene, and eye movements leave little time for sampling luminance. In addition, the amount of information reaching our visual system far exceeds the brain’s information processing capacity. Given the limited dynamic range of its neurons and its limited capacity in processing visual information in real-time, the brain deploys both structural and functional solutions that work in tandem to adapt to the surroundings. In this work, employing visual psychophysics and computational neuroscience, we study the mechanisms …

Nucleic Acids Promote Oligomerization Of Immunoglobulin G, Alexa Gomez

Electronic Theses and Dissertations

Nucleic acids have been found to prevent aggregation as chaperones, as well as act as co-factors and promote aggregation of amyloidogenic proteins leading to various diseases. Immunoglobulin G, IgG, are prone to aggregate as therapeutic proteins, and light chains of IgG can form amyloid fibrils, causing a disease known as light chain amyloidosis. Here we discuss the effect nucleic acids have on full-length immunoglobulin G aggregation. Our results show G-quadruplex DNA, and bulk DNA lead to oligomerization of full-length IgG, and induce increases in secondary structure. Tryptophan fluorescence indicates structural changes are occurring in the presence of DNA. Additionally, IgG …

Characterization Of The Whale Shark (Rhincodon Typus) Melanocortin-2 Receptor, Brianne Hoglin

Electronic Theses and Dissertations

Among bony vertebrates, the melanocortin-2 receptor ortholog is unique among the family of five melanocortin receptors on the basis that it is dependent on its accessory protein, MRAP1, for trafficking and activation, and is selective for activation by ACTH alone. Previous studies on the MC2R orthologs of select cartilaginous fish, the elephant shark (Callorhinchus milii) and the red stingray (Dasyatis akajei), revealed divergent traits in a less obligatory relationship on MRAP1 and its ability to be activated by ACTH or the MSH-sized peptides. However, observed traits were not consistent between these two cartilaginous fish species, posing …

Testing A Sociocultural Model Of Body Image In Women Athletes With Disabilities, Brooke R. Lamphere

Electronic Theses and Dissertations

Evidence suggests that athletes and people with disabilities (PWD) experience multiple body images that change relative to their social context (e.g., Petrie & Greenleaf, 2012). The powerful influence of social factors on body image and disordered eating in women athletes is well-documented (e.g., Schaefer, et al., 2015; Fitzsimmons-Craft et al., 2014), as is the centrality of the body in the lived experience of athletes and PWD (Behel & Rybarczyk, 2012; Galli et al., 2016); yet, limited research has explored the effects of social factors on body image in athletes with disabilities (i.e., AWD; e.g., Galli et al., 2016; Sousa et …

Rape: A Settler-Colonial And Anti-Black Project, Cristy A. Dougherty

Electronic Theses and Dissertations

White feminist theorizations of rape privilege patriarchy as the main source of gender violence, ultimately centering white cisgender women. In doing so, white women are treated as subject in anti-rape discourse while the violence inflicted on women of color is rendered as secondary and insignificant. Conversely, Indigenous and Black feminist analytics center Indigenous and Black women’s experiences with sexual violence, ultimately pointing to the ways in which rape has been used as a tool to perpetuate heteropatriarchy, settler-colonialism, and anti- Black racism. For instance, Deer (2015) explains that Indigenous women experience disproportionately high rates of sexual violence that spans generations. …

A Biophysical Investigation Of Hiv-1 Envelope Cytoplasmic-Tail Mediated Retention In The Gag Lattice, Nicholas Scott Groves

Electronic Theses and Dissertations

The infectivity of Human Immunodeficiency Virus is critically dependent on successful incorporation of the trimeric viral envelope glycoprotein, Envelope (Env), into Gag lattice assembly sites on the host-cell plasma membrane during virus biogenesis. The mechanistic nature of interaction between Gag, the structural protein, and Env, the viral spike, are dependent on the Gag matrix (MA) and Env cytoplasmic tail (CT) domains. Env laterally diffuses on the host cell plasma membrane until encountering a budding Gag lattice, where it is retained until particle scission and release. The mechanism of Env entry and retention in the lattice remains elusive and enigmatic. Here …

Statistical Modeling Of Positive Peer Support On Longitudinal Adolescent Substance Use, Kady Rost

Electronic Theses and Dissertations

To evaluate this study’s research question of ”Does the latent construct of Positive Peer Support (PPS) relate to the construct of Adolescent Substance Use (ASU) over time, controlling for neighborhood safety, race, and sex?”, Structural Equation (SEM) and Latent Growth Curve Modeling (LGCM) were used to investigate trajectories. Secondary longitudinal data from Zimmerman (2014) of 604 students enrolled for four consecutive years in public schools located in Flint, Michigan. In the secondary data resource, students who participated were declared “at risk” by GPA. Significant relationships were found in SEM: Positive Peer Support to Adolescent Substance Use, All Control Variables to …

Correlating With Local Variation In Collagen Level And Mechanical Properties Of The Heart Tissues, Talayeh Tavangar

Electronic Theses and Dissertations

The determination of the myocardium's tissue properties is essential in constructing finite element (FE) models of the heart. To obtain accurate results, especially for functionally modeling the heart, we must determine the tissue properties in-vivo. The calculation of the heart tissue properties remains a challenging area as it is categorized as a heterogeneous, anisotropic, nonlinear soft tissue that undergoes large deformation. In this work, we tried to introduce and evaluated a finite element method to determine the mechanical properties of the cardiac tissue. The introduced method combined a finite element modeling with the experimentally obtained images from the left and …

Excerpts From Silence – Radical Critical Feminism And Adolescent Girls’ Social Emotional Development: A Case Study Of Red River Middle School, Jessamyn Lockhart

Electronic Theses and Dissertations

The development of healthy social emotional skills is critical for adolescents to stay engaged in school, develop positive self-esteem, and engage in healthy relationships over the course of their life. Adolescent girls seem to be particularly vulnerable during the period of adolescence and attention must be paid to their specific needs. There is a call for social emotional learning in schools to be more robust (especially during a crisis like the pandemic) and more thoughtfully differentiated to meet the needs of all students. The purpose of this case study was to understand if and how school leadership in one rural …