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Rna G-Quadruplex Structures: Functions In Noncoding Rnas And Disease, Emily Benner
Rna G-Quadruplex Structures: Functions In Noncoding Rnas And Disease, Emily Benner
Electronic Theses and Dissertations
Paraspeckles are a relatively newly identified subnuclear body whose function is still largely unknown. They are membraneless organelles found within mammalian nuclei originally identified as being comprised of different paraspeckle proteins (PSPs), and later proven to also contain RNA. This study investigates the long non-coding RNA nuclear enriched abundant transcript 1 (NEAT1), which has been found to be indispensable in the formation of paraspeckles, with each containing up to 50 NEAT1 molecules. NEAT1 has two isoforms, the shorter NEAT1_1, and the longer NEAT1_2. The focus of this work will be on the NEAT1_2, which acts as a scaffold in the …
Using A Network Of Single Site-Specific Cysteine Mutations Coupled With Crosslinking Mass Spectrometry (Cx-Ms) To Refine The Structure And Dynamics Of The Human Alpha 1 Glycine Receptor (Glyr), Kayce Tomcho
Electronic Theses and Dissertations
A network of site-specific single Cys-mutations coupled with CX-MS can be used to elucidate a more refined structure of GlyR and obtain a more definitive understanding of pentameric ligand-gated ion channel (pLGIC) allostery. Each Cys-mutant is introduced into an a1 homomeric Cys null background (C41S/C290A/C345S), or in the same background with F207G/A288G mutation that allows non-desensitizing GlyR activation by ivermectin (IVM). State-dependent crosslinking with methanethiosulfonate benzophenone (MTS-bzp) to a single thiol of purified, vesicle reconstituted GlyR are conducted after enriching the receptor in different allosteric states: resting (no ligand), open (F207G/A288G + IVM), or desensitized (excess glycine). Digested peptides are …
Teachers’ Stress, Anxiety, And Depression: What Are Special Education Teachers Experiencing?, James H. Potter
Teachers’ Stress, Anxiety, And Depression: What Are Special Education Teachers Experiencing?, James H. Potter
Electronic Theses and Dissertations
Research has shown that people in the teaching profession have high levels of stress and often report increased levels of symptoms of anxiety and depression. Students’ academic progress may have adverse effects when their teachers have high-stress levels or report mental distress. Those who teach special education often face high levels of stress. However, there is little research considering special education teachers’ job-related health. This study collected data on 598 general and special education teachers in the United States. This study compared the endorsements of both stress and psychopathology between special education and general education teachers. This research found that …
K-12 Economically Disadvantaged Students, Poverty, And Education: Ecological Narratives Of Successful Raised-In-Poverty, Texas Educators, Rebecca N. Morris
K-12 Economically Disadvantaged Students, Poverty, And Education: Ecological Narratives Of Successful Raised-In-Poverty, Texas Educators, Rebecca N. Morris
Electronic Theses and Dissertations
This research study aimed to understand how successful Texas educators who grew up in poverty understood and improved the educational experience of economically disadvantaged students. This study utilized a structural ecological theory and three theories of social relationships (Social Identity, Standpoint, and Cultural Capital). This phenomenological and qualitative study used a cross-sectional, descriptive, online case study design rooted in narrative nonfiction. Virtual interviews with six successful Texas educators that grew up in poverty were conducted. A narrative method of analysis was utilized to generate codes then organize them into themes, and to construct and compare the narrative findings. The results …
Coherence, Travis Walthall
Coherence, Travis Walthall
Electronic Theses and Dissertations
COHERENCE is a series of oil paintings and pen drawings that explores open narratives, or different ways to read artwork. The paintings and drawings showcase harmony, or tension, between ambiguous and representational forms. The visual experience requires the viewer to create their own narrative and decipher forms to do so. COHERENCE is a psychological and aesthetic exhibition fueled from human experience— one of fragility, uncertainty, imperfection, beauty, faith and memories.
Simulation Of The Interaction Between Striated Muscle Unc-45 And Transcription Factor Gata-4, Drake Alexander Duncan
Simulation Of The Interaction Between Striated Muscle Unc-45 And Transcription Factor Gata-4, Drake Alexander Duncan
Electronic Theses and Dissertations
Striated Muscle UNC-45, also known as UNC-45b, is an important protein that acts as a chaperone for myosin in cardiac and skeletal muscles, binding to myosin at its C-terminal UCS domain and regulating its assembly into thick filaments and sarcomeric structures. The UCS domain contains a large loop that is believed to be the first point of interaction between myosin and UNC-45b. GATA-4 is an essential transcription factor that facilitates transcription of several genes in cardiac development, particularly alpha-heavy chain myosin in heart tissue. Recently, studies have shown that there is interaction of GATA-4 with UNC-45b and that GATA-4 binds …
The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.
The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.
Electronic Theses and Dissertations
Native bees are an important part of terrestrial ecosystems due to their coevolution with flowering plants. This study catalogued the bee fauna of two sandyland sites in the Big Thicket National Preserve and assessed whether a community was impacted by a historic hurricane-induced flooding event. It was hypothesized that a change in diversity metrics would be evident following the flood. Datasets were analyzed for differences in species richness, abundance, evenness, and Shannon’s diversity. Similarities between datasets were also assessed using the multivariate tests analysis of similarity (ANOSIM) and similarity of percentages (SIMPER). At two sites over two years of sampling …
Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk
Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk
Electronic Theses and Dissertations
The natural environment can be negatively impacted by a variety of human activities, including the production of artificial light at night and anthropogenic noise. Recent studies suggest that pollution from anthropogenic light and noise alters animal behavior. Despite being highly nocturnal and vocal animals, little attention has been given to anurans and the effects artificial light at night and anthropogenic noise have on their behavior. This study investigated the effects of artificial light at night and anthropogenic noise on anuran breeding systems in eastern Texas. Specifically, this study investigated whether (1) artificial light and anthropogenic noise altered calling behavior in …
Harnessing The Power Of Trained Immunity In The Setting Of Pancreatic Cancer: A Novel Mechanism Of Immune Trafficking And Tumor Control., Anne Elena Geller
Harnessing The Power Of Trained Immunity In The Setting Of Pancreatic Cancer: A Novel Mechanism Of Immune Trafficking And Tumor Control., Anne Elena Geller
Electronic Theses and Dissertations
Despite the success of immunotherapy in many types of cancer, pancreatic adenocarcinoma (PDAC) has yet to benefit. Innate immune cells are critical to antitumor immunosurveillance and recent studies have revealed that these populations possess a form of memory, termed trained innate immunity, which occurs through transcriptomic, epigenetic, and metabolic reprograming. Though trained innate immunity has mostly been investigated in the context of infection, the induction of trained innate immunity could also protect against tumors, and specifically pancreatic tumors. Here, we demonstrate that yeast-derived particulate β-glucan, a known inducer of trained immunity, traffics to the pancreas following IP administration. This causes …
Ecological, Evolutionary, And Molecular Mechanisms Driving Pyocin Diversity In Pseudomonas Aeruginosa., Aubrey A. Mojesky
Ecological, Evolutionary, And Molecular Mechanisms Driving Pyocin Diversity In Pseudomonas Aeruginosa., Aubrey A. Mojesky
Electronic Theses and Dissertations
Bacteriocins are narrow-spectrum antibiotics produced in nearly all lineages of bacteria, meaning that these antimicrobials target closely related individuals. The bacteriocins of Pseudomonas aeruginosa, called pyocins, are highly prevalent and diverse in populations of this species. Laboratory studies have shown that pyocins can function to mediate the outcome of interactions, often allowing for the coexistence of multiple strains, as no one pyocin genotype is competitively superior. Although this has been demonstrated under laboratory conditions, the function of pyocins in natural settings and the ecological, evolutionary, and genetic mechanisms underlying pyocin diversity remains unclear. As such, for my dissertation, I …
Identifying The Cardiovascular Effects Of Multiple Pollutants., Katlyn Elizabeth Mcgraw
Identifying The Cardiovascular Effects Of Multiple Pollutants., Katlyn Elizabeth Mcgraw
Electronic Theses and Dissertations
Cardiovascular disease (CVD) is the leading cause of death from environmental exposures. Although exposure to PM2.5 is an established risk factor for CVD, the contribution of other hazardous pollutant exposure to CVD is less clear. Overall, this work aimed to examine the effect of pollutants with lesser documented effects on cardiovascular disease using a multi-pronged approach to exposure assessment. The three aims were to examine the relationship between county-level toxic chemical releases and CVD mortality in the contiguous United States between 2002 and 2012, to assess the relationship between individual-level VOC metabolites and vascular function, and to build multipollutant …
A Home For Friendless Women With Critical Essay "But What Was She Wearing?", Kelly E. Hill
A Home For Friendless Women With Critical Essay "But What Was She Wearing?", Kelly E. Hill
Electronic Theses and Dissertations
This dissertation is a historical novel accompanied by a critical lyric essay. Through a combination of scholarly research and creative activity, the novel reimagines the lives of the women who lived as inmates and administrators in the Home for Friendless Women, a charity home that operated in Louisville in the late nineteenth century. The critical essay draws from my interdisciplinary background to combine personal experiences, archival and scholarly research, and literary analysis to connect the U.S. dress reform movement in the 1850s, and its failure to change public perception about what was acceptable attire for middle and upper class women, …
Computational Frameworks For Microrna Functional Analysis Of Inter-Kingdom And Indirect Targeting., Mohammed Sayed
Computational Frameworks For Microrna Functional Analysis Of Inter-Kingdom And Indirect Targeting., Mohammed Sayed
Electronic Theses and Dissertations
Genes are DNA sequences that encode the information needed to synthesize molecules necessary for the function of the cell. Some genes are called protein-coding genes because they have the code required to manufacture proteins. The expression of a certain gene means its product (protein) is produced. Although some genes are not protein-coding, they regulate the gene expression of other protein-coding genes. Of these, microRNAs (miRNAs) are small RNA molecules that inhibit the expression of other genes by binding to their mRNA transcripts. miRNAs have been shown to be linked to several biological processes like development and diseases like cancer. Recently, …
Major League Baseball Fans’ Climate Change Attitudes And Willingness To Adapt: Climate Vulnerability Vs. America’S Pastime., Jessica R. Murfree
Major League Baseball Fans’ Climate Change Attitudes And Willingness To Adapt: Climate Vulnerability Vs. America’S Pastime., Jessica R. Murfree
Electronic Theses and Dissertations
Climate change threatens the ability to enjoy sport around the world, including in the United States. While the scientific community reached consensus regarding the presence and severity of climate change near the turn of the twenty-first century, that same agreement has not been met across the American general public. Major League Baseball (MLB) is particularly vulnerable to climate change in the U.S. due to its season duration, geographic footprint, and largely outdoor nature. Therefore, the purpose of this study was to investigate relationships between U.S.-based MLB fans’ sport identification and their climate change attitudes, perceptions of climate change risk, and …
Machine Learning Approaches For Lung Cancer Diagnosis., Ahmed Mahmoud Ahmed Shaffie
Machine Learning Approaches For Lung Cancer Diagnosis., Ahmed Mahmoud Ahmed Shaffie
Electronic Theses and Dissertations
The enormity of changes and development in the field of medical imaging technology is hard to fathom, as it does not just represent the technique and process of constructing visual representations of the body from inside for medical analysis and to reveal the internal structure of different organs under the skin, but also it provides a noninvasive way for diagnosis of various disease and suggest an efficient ways to treat them. While data surrounding all of our lives are stored and collected to be ready for analysis by data scientists, medical images are considered a rich source that could provide …
From Byronic To Gothic Blood Sucker: Subversion Toward A Non-Gendered Identity, Hannah Hoover
From Byronic To Gothic Blood Sucker: Subversion Toward A Non-Gendered Identity, Hannah Hoover
Electronic Theses and Dissertations
Analyzing Emily Bronte’s Wuthering Heights and linking trends of the Byronic hero that have merged into a variety of genres reveal that the hero is a mode of subversive gender expression, which has evolved within the Gothic through feminine desire. Delving into Bram Stoker’s Dracula will provide unique insight into the audience’s desires/expressions of gender. Finding the transition point from the monster vampire of Dracula to Stephanie Meyer’s desirous, sparkling boy-next-door in Twilight will track the trajectory of gender and sexual norms through time. From the foundational adaptation of the Byronic hero in Wuthering Heights to the repressed vampiric desire …
The Rivers Of Life: A Portraiture Study Of Three Black Female Educational Leaders In A Large Urban School District., Cassandra G. Woods
The Rivers Of Life: A Portraiture Study Of Three Black Female Educational Leaders In A Large Urban School District., Cassandra G. Woods
Electronic Theses and Dissertations
This dissertation is a qualitative portraiture study that explores Black female educational leaders’ lived experiences and the way those experiences cultivate knowledge formation. This study uses Black feminist thought as a theoretical framework. Black feminist thought grounds this research study in advancing intellectual traditions, equal educational opportunities, and the ethic of personal accountability. Black feminist thought serves as an ideal theoretical framework to ground this research study because its utility in clarifying the unique way Black female educational leaders translate theory into action (Loder, 2005; Lomotey, 1993; Tillman, 2008; Renix, 2016). The findings of this research study position Black female …
Pde4 Inhibition: A Novel Therapeutic Strategy In Liver Fibrosis., Mohamed Elnagdy
Pde4 Inhibition: A Novel Therapeutic Strategy In Liver Fibrosis., Mohamed Elnagdy
Electronic Theses and Dissertations
Background: Liver fibrosis is accumulation of extracellular matrix (ECM) proteins due to chronic liver injury. Chronic hepatic damage can occur due to multiple causes including, alcohol, non-alcoholic steatohepatitis and chronic viral hepatitis. Liver fibrosis is a critical problem worldwide due to the extremely high incidence of alcohol associated liver damage and chronic viral hepatitis. Moreover, there is no FDA approved therapy for liver fibrosis. Activation and transdifferentiation of quiecent hepatic stellate cells (HSCs) to myofibroblasts (MFBs) is the main event contributing to liver fibrosis. This process involves two phases, the initiation and the perpetuation phases. Hepatocyte injury and inflammatory response …
The Neural Architecture Of Emotional Intelligence., Teodora Stoica
The Neural Architecture Of Emotional Intelligence., Teodora Stoica
Electronic Theses and Dissertations
Emotional Intelligence (EI) is a nebulous concept that permeates daily interpersonal communication. Despite prolific research into its benefits, EI subjective measurement is difficult, contributing to an enigmatic definition of its core constructs. However, neuroimaging research probing socioaffective brain mechanisms underlying putative EI constructs can add an objective perspective to existing models, thereby illuminating the nature of EI. Therefore, the primary aim of this dissertation is to identify brain networks underlying EI and examine how EI arises from the brain’s functional and structural neuroarchitecture. EI is first defined according to behavioral data, which suggests EI is made up of two core …
Habitation And Interaction In The Lower Illinois River Valley: A Case Study On Household Structure And Ceramics At The German Site (11c377)., Catherine Zoe Doubles
Habitation And Interaction In The Lower Illinois River Valley: A Case Study On Household Structure And Ceramics At The German Site (11c377)., Catherine Zoe Doubles
Electronic Theses and Dissertations
The purpose of this project is to investigate the less understood narrative of the Late Woodland peoples at the German site within the Lower Illinois River Valley. In order to determine the extent of Mississippian cultural interaction with the Jersey Bluff Phase, Late Woodland, peoples, I will examine the houses and artifacts found during the 2019 field season by the Centre for American Archeology. Data from the geophysical surveys, notes from the site excavations, and ceramic analysis will be used to place the German site within the theoretical framework and archaeological context of the region. In the end, the objective …
Presence Of Severe Acute Respiratory Syndrome Coronavirus 2 In Urban Streams Receiving Sewer Overflow, Louisville, Kentucky, Usa., Cullen Hunter
Presence Of Severe Acute Respiratory Syndrome Coronavirus 2 In Urban Streams Receiving Sewer Overflow, Louisville, Kentucky, Usa., Cullen Hunter
Electronic Theses and Dissertations
Pathogens may enter surface waters as they are shed in human feces and potentially delivered to surface waters via sewer overflows, particularly in the eastern United States. This study examined of the presence of fecal indicators in two forks of Beargrass Creek in Louisville, Kentucky. Surface water grab samples (N=30), sediment samples, water quality, and a paired wastewater surveillance study were analyzed. SARS-CoV-2 was detected in a single stream sample despite ubiquitous presence of the virus within the area in wastewater and consistent evidence of a human fecal indicator. These findings demonstrate the need for more green and gray infrastructure …
Particulate Matter Exposure And Community-Acquired Pneumonia Mortality Among Hospitalized Adults With Pneumococcal Pneumonia: Incidence Study (Happi) Participants., Jack Anthony Pfeiffer
Particulate Matter Exposure And Community-Acquired Pneumonia Mortality Among Hospitalized Adults With Pneumococcal Pneumonia: Incidence Study (Happi) Participants., Jack Anthony Pfeiffer
Electronic Theses and Dissertations
The role of air pollution as an agent of elevated morbidity and mortality has come under increasingly intense scrutiny in recent years. As the body of literature grows, so too does our understanding of how extensive the harm of such pollutants can be. Particulate matter (PM) particularly harmful due to its small size, ability to travel great distance while airborne, and capacity to infiltrate numerous bodily systems, often to the detriment of those exposed. Besides being associated with increased mortality risk in more heavily exposed populations, PM exposure has also been associated with complications in the respiratory, cardiovascular, and nervous …
Saving Butterflies In The City: Gardens As Conservation Spaces In Urban Landscapes., Lindsay D. Nason
Saving Butterflies In The City: Gardens As Conservation Spaces In Urban Landscapes., Lindsay D. Nason
Electronic Theses and Dissertations
Butterfly populations are declining, and habitat degradation due to urbanization is a major contributing factor. Gardens represent a large proportion of land area in many cities, and thus may be important for conserving butterflies in urban environments. In this dissertation, I examine how garden features (ex: size, level of urbanization, plant diversity) affect adult butterfly diversity/abundance, behavior, and the predation risk faced by caterpillars. My study sites were native plant gardens in Jefferson, Bullitt, and Hardin Cos.,KY. In Chapter One, I used clay caterpillars to assess differences in predation pressure in gardens along a gradient of percent impervious surface (%IS). …
Harmful Algal Blooms In The Ohio River: Spatial And Temporal Differences., Bevin Hardy
Harmful Algal Blooms In The Ohio River: Spatial And Temporal Differences., Bevin Hardy
Electronic Theses and Dissertations
The frequency of harmful algal blooms (HABs) has been increasing across the globe, especially in river ecosystems. Although more common in lakes, more HABs have been reported in rivers in the past decade, particularly in the Ohio River. In 2019, a HAB stretched 200 to 300 miles throughout the Ohio River from September to October. Samples were collected for the duration of the 2019 bloom (August-October) in the McAlpine Pool in Louisville, KY to compare microcystis toxin (microcystin) concentrations on a temporal and spatial scale. Different habitats (bay, creek, river) were sampled throughout the bloom, and two additional sampling days, …
The Peacock In The Room: Confronting The Hidden Curriculum Of Androcentrism And Gender Bias In Undergraduate Biology Education., Sarah Hamilton Spaulding
The Peacock In The Room: Confronting The Hidden Curriculum Of Androcentrism And Gender Bias In Undergraduate Biology Education., Sarah Hamilton Spaulding
Electronic Theses and Dissertations
This dissertation exposes one manifestation of the hidden curriculum of gender bias in the biological academe and explores the impacts of implicit essentialist perspectives on biology curricula and student understanding of an important evolutionary concept. Chapter one introduces the concept of gender essentialism and its relationship to the hidden curriculum of gender bias. I conclude the introductory chapter by reviewing tenets from queer curriculum theory and suggesting that applications of the theory may provide educators with the pedagogical tools required to counter implicit essentialist perspectives. Chapter two describes a textbook image analysis informed by previous textbook analyses to examine the …
Determination Of The Biological Functions Of Undefined Domains Of Veev Nsp2., Andrew Michael Skidmore
Determination Of The Biological Functions Of Undefined Domains Of Veev Nsp2., Andrew Michael Skidmore
Electronic Theses and Dissertations
Alphaviruses are positive sense, single strand, RNA viruses. These viruses occur on every populated continent. Alphaviruses are divided into two clades, the New-World and Old-World viruses. The New-World viruses include Eastern (EEEV), Western (WEEV), and Venezuelan equine encephalitis viruses (VEEV), and cause neuroinvasive disease. The Old-World viruses include Chikungunya (CHIKV) and Ross River viruses (RRV), and typically cause multijoint arthralgia. There are currently no approved antiviral therapeutics or vaccines for any alphavirus, making them a high priority for antiviral drug design and discovery. A benzamidine inhibitor (ML336) of VEEV was characterized, and determined to inhibit replication of VEEV RNA during …
Chronic Codeswitching: A Phenomenological Study Examining Multiracial Student Sense Of Belonging In A Predominantly White Institution., Nicholas Lamar Wright
Chronic Codeswitching: A Phenomenological Study Examining Multiracial Student Sense Of Belonging In A Predominantly White Institution., Nicholas Lamar Wright
Electronic Theses and Dissertations
Constantly feeling a lack of acceptance and getting the comment “You are too Black” or “You are too White” is a challenging, common occurrence for multiracial students, but especially those in predominantly White institutions. This is just one of the barriers that stand between multiracial students and forming a sense of belonging at a predominantly White institution. The majority of research examining sense of belonging focuses on either Black or White students, but neglect multiracial students and their experiences. This dissertation examines sense of belonging for multiracial (Black/White) students in a predominantly White institution, by interviewing 11 multiracial students at …
Genetic And Functional Analysis Of Rat Mammary Cancer Susceptibility., Emily L Duderstadt
Genetic And Functional Analysis Of Rat Mammary Cancer Susceptibility., Emily L Duderstadt
Electronic Theses and Dissertations
Breast cancer genetic susceptibility is modulated by high to low penetrance risk alleles. Population frequencies of high and moderate penetrant predisposing mutations are rare and responsible for a small percentage of population cases. Most genetic variation in breast cancer susceptibility is low penetrance common variants where causal genes and risk-associated mechanisms are largely unknown. Comparative genetics uses animal models to isolate and mechanistically evaluate genetic susceptibility to human disease. Rat strains have different susceptibility phenotypes to chemical-induced carcinogenesis. Selective breeding and genetic linkage analysis using a 7,12-dimethylbenz(a)anthracene (DMBA) rat mammary carcinogenesis model have identified Mammary carcinoma susceptibilityquantitative trait loci …
A Qualitative Look At Secondary Trauma Through The Lived Experiences Of Elementary Teachers., Terri L Davenport
A Qualitative Look At Secondary Trauma Through The Lived Experiences Of Elementary Teachers., Terri L Davenport
Electronic Theses and Dissertations
This research study examined the lived experiences of elementary educators working with traumatized students. Secondary trauma among other professions such as social workers and first responders is well-documented (Bride et al., 2007). Research on the impact it has on educators is still limited (Hill, 2011). This dissertation provides evidence that educators are directly impacted by the work that they do to support and educate their students. A phenomenological approach is used to explore the troubling personal challenges educators face while working with traumatized students. The study examines elementary teachers’ experiences using the Secondary Traumatic Stress (STS) lens as a theoretical …
Isomotive Dielectrophoresis For Enhanced Analyses Of Cell Subpopulations., Mohamed Zakarya Rashed
Isomotive Dielectrophoresis For Enhanced Analyses Of Cell Subpopulations., Mohamed Zakarya Rashed
Electronic Theses and Dissertations
As the relentless dream of creating a true lab-on-a-chip device is closer to realization than ever before, which will be enabled through efficient and reliable sample characterization systems. Dielectrophoresis (DEP) is a term used to describe the motion of dielectric particles/ cells, by means of a non-uniform electric field (AC or DC). Cells of different dielectric properties (i.e., size, interior properties, and membrane properties) will act differently under the influence of dielectrophoretic force. Therefore, DEP can be used as a powerful, robust, and flexible tool for cellular manipulation, separation, characterization, and patterning. However, most recent DEP applications focus on …