Digital Commons Network^™

Forgiveness, Gratitude, Humility, And Spiritual Struggle: Associations With Religious Belief Status And Suicide Risk, Benjamin B. Hall

Electronic Theses and Dissertations

Religion is a known contributor to suicide risk, with both positive and negative effects. Negative religious experiences, such as spiritual struggle, can exacerbate suicide risk. Alternatively, religion may promote positive psychological characteristics associated with reduced suicide risk, such as forgiveness, gratitude, and humility. However, research has yet to assess how religious changes, including conversion and deconversion, affect the linkage between religious risk and protective factors and suicidal behavior. We conducted three studies assessing these associations across four belief status groups: life-long religious believers, former religious non-believers who now believe, life-long religious non-believers, and former religious believers who no longer believe. …

Neuroinflammation, Peripheral Inflammation And Gut Microbiome Profiles In Male Mice From Two Proposed Mouse Models Of Social Behavior Deficits, Sarah Parkinson

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is a neurodevelopmental disorder marked by social deficits and repetitive actions. A communication pathway exists between the brain and gut called the gut-brain axis. It is thought that gut bacteria can secrete signaling molecules, triggering inflammation across the body. These studies attempt to determine if markers are expressed in two mouse models of ASD behaviors, BTBR and a valproic acid model. Immunohistochemistry of ionized calcium binding adaptor molecule 1 from male mouse brain tissue showed no microglial activation in any group. Cytokine analysis did exhibit an increase in interleukin 1 (IL-1) in male adult mice only. …

Gabaergic-Related Pathology In The Anterior Cingulate Cortex Of Postmortem Human Brain Tissue In Autism Spectrum Disorder, Gethien Andrew

Electronic Theses and Dissertations

The anterior cingulate cortex (ACC) is part of the cognitive and emotional brain circuitry that mediates social interaction. Imbalances between inhibitory, GABAergic neurons, and excitatory, glutamatergic neurons, in this region are essential to brain circuity during social responses and are thought to be involved with behaviors associated with autism spectrum disorder (ASD). Enriched cell populations of glutamatergic neurons, obtained through laser capture microdissection, were used for gene expression studies of GABAergic receptors (GABRA1, GABRA4, and GABBR1). Additionally, proteins that impact GABAergic synapses (Spinophilin, CPLX1, mTOR, IGF1R, PSD95, PARP1) were investigated using Western Blotting with punchdissected homogenate brain tissue from ACC …

Endothelial Heat Shock Protein A12b And Yes-Associated Protein Cooperatively Promote Angiogenesis Following Myocardial Infarction, Min Fan

Electronic Theses and Dissertations

Heart failure after myocardial infarction (MI) remains the leading cause of mortality among all cardiovascular diseases globally. Angiogenesis plays a critical role in cardiac functional recovery after MI. Heat shock protein A12B (HSPA12B) is predominately expressed in endothelial cells and required for angiogenesis. Yes-associated protein (YAP) has been reported to promote tumor angiogenesis.

In the present study, we investigated the cooperative role of HSPA12B and YAP in angiogenesis following myocardial ischemic injury. Endothelial specific deficiency of HSPA12B (eHspa12b^-/-) or YAP (eYap^-/-) impairs angiogenesis and exacerbates cardiac dysfunction after MI, when compared with wild type …

Characterization Of Sabp2-Interacting Proteins (Sip) 428: An Nad+-Dependent Deacetylase Enzyme In Plant Abiotic Stress Signaling, Oviavo Nohoesu

Electronic Theses and Dissertations

Abiotic stress leads to a change in the water content of plants. Salinity and osmotic stress affect both the morphology and physiology of plants. Plants have therefore responded to these environmental changes by adapting and tolerating them. The SABP2-interacting proteins (SIP) 428-silenced RNAi transgenic tobacco lines were subjected to various abiotic stresses (salinity, osmotic, and drought). The effect of SIP428-silencing on the tobacco plants subjected to these abiotic stresses was monitored. The results from the root growth data show that the sip428-silenced lines exhibit enhanced tolerance to the stressors compared to the wild-type plants. Interestingly, results of the relative chlorophyll …

An Electrochemical Immunoassay System For Measuring Circulating Protein Biomarkers Of Pediatric Soft Tissue Sarcoma, Ivy Antwi

Electronic Theses and Dissertations

Measurement of circulating protein biomarkers associated with disease can facilitate early detection, help guide treatment strategies and improve patient outcomes beyond current standards of care. The combination of inexpensive 3D-printed flow cells and electrochemical biosensors has recently emerged as a viable platform for low-cost, reliable biomarker measurements. Here, we report an electrochemical immunoassay system based on simple graphite electrode arrays, 3D-printed flow cells, and signal-generating magnetic bead bioconjugates for simultaneous detection of three biomarker proteins (cancer antigen 125 (CA-125), midkine (MK) and osteopontin (OPN)) associated with pediatric soft tissue sarcomas (PSTS). Magnetic bead bioconjugates are functionalized with large amounts of …

Athlete Monitoring Program In Division I Collegiate Female Soccer, Ai Ishida

Electronic Theses and Dissertations

The objectives of this dissertation include 1) to review athlete monitoring strategies and the physical performance demands of female soccer match-play and to provide practical application of athlete monitoring programs, 2) to examine individual and group relationship between training load (TL) and subjective recovery and stress state and neuromuscular performance, and 3) to investigate acute effects of match-play on neuromuscular and subjective recovery and stress state in National Collegiate Association Athlete (NCAA) division I collegiate female soccer. TL was assessed using 10Hz Global Navigation Satellite System units. Subjective recovery and stress state was measured using the Short Recovery and Stress …

First-Year Seminar And Its Impact On Retention At A Small Liberal Arts College In South Carolina, Michael A. Smith

Electronic Theses and Dissertations

First-Year Seminar (FYS) courses have been the foundational building blocks for academic and nonacademic success for first-year college freshman since the late 1970s. With the focus on improving retention and persistence, FYS courses have been viewed as the resource that connects freshmen to the faculty, staff, student, and campus environment. Therefore, it is vital to see the impact that it has had on students and how it can further develop persistence through graduation. The researcher conducted this basic qualitative study to look for emerging themes of sophomore experience in the FYS courses and how that impacted their persistence and retention …

Medication Versus Brain-Based Treatment: Evaluation Treatment Preferences Of Parents Of Children With Adhd, Rebecca Recio-Swift

Electronic Theses and Dissertations

Attention-Deficit/Hyperactivity Disorder (ADHD) is one of the most prevalent neurodevelopmental disorders across the world. Currently, treatment for ADHD mostly consists of either medication or behavioral therapy or a combination of both. However, research has shown that medication used as therapy for the treatment of ADHD has side effects which parents deem undesirable for their children. Therefore, recent research has focused on patient and parent preferences. Studies have found that behavioral or other treatment options may often be chosen over medications. These same studies have documented the characteristics of parents that prefer certain treatments for their children. The purpose of this …

Analysis Of Disproportionality In Discipline And The Effects Of Positive Behavior Supports Within Special Education, Cutler T. Ruby

Electronic Theses and Dissertations

Nationwide, school districts are required (IDEA, 2004) to implement positive behavior interventions and supports (PBIS) for all students receiving special education services. These PBIS are reported to reduce problem behaviors and increase prosocial behaviors when implemented with fidelity (Crone et al., 2015). With a reduction of problem behaviors an expected reduction of discipline referrals should follow along with a reduction in days spent in exclusionary discipline for students in special education. Reducing days in exclusionary discipline is desired due to a strong research base linking the practice to several negative outcomes (Marchbanks et al., 2015) along with data demonstrating these …

Molecular Dynamics Simulations Provide Insight Into Stability Of Hyperthermophilic Endoglucanases, Logan E. Sheffield

Electronic Theses and Dissertations

Endoglucanases play a key role in the industrial production of bioethanol, but the most efficient method requires the utilization of high temperatures and is currently limited by the thermostability of endoglucanases. For this reason, it would be beneficial to discover more high-efficiency, thermostable enzymes to utilize in the hydrolytic process. In this study molecular dynamics simulations were performed on structurally similar endoglucanases with varying levels of thermostability to gain insight on what factors contribute to thermostability in endoglucanases. RMSD, RMSF, PCA, hydrogen bonding and salt bridges were analyzed. Finally, protein energy networks were constructed from nonbonded interaction potentials and analysis …

Precambrian Basement Influence On The Deposition Of The Upper Ordovician Utica Shale Play In East Central Ohio, William Kaleb Kirk

Electronic Theses and Dissertations

The Ordovician Utica shale play is a major oil and gas producing interval in the Appalachian Basin. The Utica shale play can be found as far as New York and Canada and to the south into Indiana and Kentucky. The play consists of the Trenton/Lexington limestones, Point Pleasant Formation, and Utica shale. The shallow marine fossiliferous limestones of the Trenton and shallow marine shaley limestones of the Lexington are overlain by an interbedded shale and limestone of the Point Pleasant Formation, which grade into the deeper marine interbedded shales and limey shales of the Utica. These formations are highly heterogeneous, …

Targeting The Mek5, Mek1/2, And/Or Pi3k Pathways To Reverse Epithelial To Mesenchymal Transition And Enhance Chemotherapeutic Sensitivity In Breast And Brain Cancers, Akshita Bhatt

Electronic Theses and Dissertations

Triple-negative breast cancer (TNBC) is the most aggressive form of breast cancer and accounts for 10-15% of breast cancer cases. Due to absence of estrogen, progesterone, and human epidermal growth factor receptors, there is lack of targeted therapies and chemotherapy remains the mainstay treatment. Moreover, drug resistance is a major problem associated specifically with TNBC. Similarly, while estrogen receptor positive (ER+) breast cancer can be treated with targeted therapies such as tamoxifen, 40% of patients develop resistance and resulting in recurrence of the disease.

There is a dire need for identifying novel targets and developing therapeutics to target triple-negative and …

Clinical Pharmacy Services And Medication Utilization In Hospice Care, Aishwarya Kulkarni

Electronic Theses and Dissertations

Background: As discussed within the guidelines from the American Society of Health-System Pharmacists (ASHP), pharmacists are an integral part of the hospice multidisciplinary team involved in optimizing the treatments. Methadone, a long-active opioid, is particularly useful in this population but may be clinically underutilized. Additionally, the cost share of medication utilization in hospice and palliative care is shifting towards hospice providers. The assessment of medication utilization and methadone use in this setting can help develop overall cost/clinical optimization strategies. Thus, there is a need to understand the use and expenditure of various medications and pharmacists' role in providing methadone …

Predicting Covid-19 Behavior: Is It All Due To Political Orientation?, Caleb Potts

Electronic Theses and Dissertations

ABSTRACT

Adherence to COVID-19 preventing measures is becoming increasingly important as governments across the world realize COVID-19 is not an acute, but a chronic problem. There is, however, disagreement over to what extent COVID-19 is a problem. In the United States, the division appears to be primarily along party lines (Democrat and Republican[1]), though even within parties there is division. This division might be explained by differences in: the behavioral immune system and trust in government. Additional factors to examine include: personality, fear of COVID-19, and religious beliefs. The present study used previously validated self-report measures to assess …

Food-Web And Functional Trait Community Structure Of Predator Assemblages In Pine Forests Under Different Management Regimes, Connor Adams

Electronic Theses and Dissertations

Anthropogenic activities can alter natural disturbance regimes in ecosystems, and thereby affect the structure and function of biological diversity. As many of the world’s ecosystems are degraded beyond natural recovery, well-defined restoration goals are necessary to maintain the ecological processes that provide valuable ecosystem services. Utilizing taxonomic, functional, and food-web approaches, I investigated the impacts of forest management practices in structuring predator communities in two pine forest systems of eastern Texas. The results of this study indicate that the increased frequency of forest management practices such as prescribed fires and thinning operations encourages predator diversity while increasing functional and trophic …

Machine Learning Applied To Colloidal Properties Of Perfluorocarbon Nanoemulsions For Imaging In Ards/Ali, Marco Hosfeld

Electronic Theses and Dissertations

Acute Respiratory distress Syndrome (ARDS) and Acute Lung Injury (ALI) are inflammatory lung pathologies consisting of non-hydrostatic pulmonary edema leading to hypoxia and impaired gas exchange in the lungs. ARDS/ALI is both difficult to study and treat as it is not in itself a specific pathology but rather a syndrome consisting of many pathologies that vary case by case. It is, however, consistently characterized by an explosive acute inflammatory response in the lung parenchyma leading to hypoxia. Although time has seen to an increase in the understanding of ARDS/ALI, the mortality rate remains in the range of 30-50%. For these …

Computational And Experimental Studies Of Functionalized Phenolic Tertiary Phosphine Oxides As Organocatalysts For The Redox-Neutral Mitsunobu Reaction, Sadie Brown

Electronic Theses and Dissertations

The purpose of this thesis was the identification of a more efficient catalyst analog that could perform the redox-neutral Mitsunobu reaction, which was achieved via two synthetic pathways: elimination-addition and addition-rearrangement. Furthermore, a computational investigation was conducted on the conflicting interpretations of the catalytic cycle. Our research group replicated Houk et al. work, as well as expanded the computations to examine the influence different functional groups had on the ground state and transition state structures. Important ground state and transition state structures were determined, which provided a more energetically favorable pathway of the catalytic cycle. Experimental work demonstrated that both …

Get Ready For Future Education: A Phenomenological Study On Artificial Intelligence, Son T. H. Pham

Electronic Theses and Dissertations

Artificial intelligence (A.I.) brings new opportunities for social construction. This phenomenological research attempted to analyze the phenomenon of artificial intelligence in education, grounding in American philosophy, with a review of the history of A.I. in education, its promise, and possible futures. The primary purpose of the study was to share voices and lived experiences of educators, philosophers, industrialists and politicians toward the coming high-tech society. Fundamental to this philosophical inquiry was Heideggerian interpretive phenomenology. The findings from eight intellectuals participating in this study elucidated five themes of the investigated phenomenon. Together these empirical indicators, and an outline of philosophy of …

Empowering Mothers: A Conceptual Model For Promoting Perinatal Mental Wellness Among Women In The United States, Kayla Acklin

Electronic Theses and Dissertations

Globally, there are certain expectations surrounding becoming a mother related to pregnancy and the birth. For many women, the birth of a child represents a natural event filled with joy. Societally, the expectation is that pregnancy, birth, and the transition into motherhood are normal, positive experiences for all women (Davis-Floyd & Cheyney, 2019). However, research shows that some women develop impairments in physical and mental health functioning and wellbeing as a result of the major physical and psychosocial changes that take place during the transition into motherhood (Austin et al., 2010; Ayers & Pickering, 2001; O’Hara et al., 2014).

This …

Detecting A Heterogenous Sample Of Pigmented Melanoma Cell Lines Using Photoacoustic Flow Cytometry, Margaret Cappellano

Electronic Theses and Dissertations

Metastatic melanoma is the deadliest form of skin cancer, which is in part, attributed to its rapid aggression and lack of response to typical treatment methods. There are far too often cases where a lymph node biopsy does not detect the severity of the cancer, which in turn causes a lack of diagnosis until a mass can be visually detected on a scan, such as a PET, CT, or MRI. Once visible on a scan, the cancer is too progressive for successful treatment. To avoid this, we investigated how a blood sample can be used to negate a missed diagnosis, …

An Interpretive Phenomenological Analysis Of Psychotherapists' Experiences Of Vulnerability, Kay Yu Yuan Chai

Electronic Theses and Dissertations

In the service of forging a deep authentic connection that has the power to heal and transform, psychotherapists create and hold space for their patients to show themselves in a deeply vulnerable manner so that they can be known and accepted as they are. At the same time, psychotherapists are also flawed and wounded mortal beings who cannot help but bring their own woundedness, personalities, and limitations into the space, and must negotiate the delicate balance between restraint and expressiveness of their vulnerability. This study is a phenomenological inquiry into how psychotherapists experience their vulnerability given the demands of their …

Quantification Of Persistent Organic Pollutants In Various Matrices Using Stir Bar Sorptive Extraction And Isotope Dilution Mass Spectrometry, Weier Hao

Electronic Theses and Dissertations

A method was developed to quantify persistent organic pollutants (POPs) in a wide range of matrices including wastewater, dietary supplements, and human whole blood using stir-bar sorptive extraction, GC-MS/MS, and isotope dilution mass spectrometry (IDMS). The method enabled accurate, precise, sensitive, and efficient quantification of POPs in these matrices. Compared with calibration curves, IDMS provided measurements with a higher level of accuracy and precision, especially at lower measured concentrations. The use of GC-MS/MS enabled a lower limit of quantification compared with GC-MS. A reverse-IDMS method was performed to further eliminate biases from the labelled concentrations of the commercially available standards. …

Air Pollution In The Steel City: Assessing The Influence Of Covid-19 On Air Pollution In Allegheny County, Carissa L. Lange

Electronic Theses and Dissertations

Though the novel coronavirus (COVID-19) has sickened millions of individuals, lockdown measures have provided a unique opportunity to analyze air pollution reductions. Reductions in air pollution are especially important in a city like Pittsburgh, where industrial activity has led to elevated levels of particulate matter with an aerodynamic diameter 2.5). The majority of this PM_2.5 pollution comes from two industrial facilities, the Edgar Thomson Steel Works and the Clairton Coke Works. By utilizing a natural experiment, this study sought to determine how large of a role the COVID-19 lockdowns played in improving air quality in the Pittsburgh region. Data …

Professional Ethics For College Health Services, Dessa Mrvos

Electronic Theses and Dissertations

College health services provide a crucial resource for campus communities in the United States, substantially affecting student health, safety, and wellbeing. The impact and quality of campus clinical services impact student life through varied approaches to the delivery of services, health innovations, and business models. The role that campus health services plays in dealing with risk mitigation is essential: well-established public health principles of disease prevention, health promotion, and health education are sustained and advanced by campus clinical health services. Despite the widely recognized importance of college health services, there is very little literature on the contribution of ethics related …

Diagnosis Of Melanoma Disease State From Patient Blood Samples Using Photoacoustic Flow Cytometry, Jacob Salvatore

Electronic Theses and Dissertations

Approximately 1 in 50 Americans develop melanoma in their lifetime. Early detection of melanoma is pivotal to the survival of the patient, with a 99% survival rate for 5 or more years after an early diagnosis. Metastasis, or the spread of cancer, increases the chances of cancerous growth in other parts of the body. In this study, we propose the use of photoacoustic flow cytometry as a diagnostic of patient disease state, by closely monitoring the amount of circulating melanoma cells (CMCs) in a patient’s blood before and after chemotherapy treatments. Using this patient data, we were able to make …

Characterizing The Dormancy And Repair Of A Circumneutral Passive Remediation System Receiving Iron And Sulfate-Rich Amd, Garrett Struble

Electronic Theses and Dissertations

The Wingfield Pines remediation system is a ~20-acre circumneutral, gravity-fed system constructed in 2009 to passively remediate Fe from an ~2000 gallon/minute effluent. In 2017, a fracture to the system’s underground cavern caused the AMD effluent to bypass the system, flowing directly into Chartiers Creek and leaving the system decommissioned between 2017-2019. Soil slurry and water samples were collected in the weeks prior to the system’s reinstalment in September 2019 through January 2020 in order to determine the effect of the repair on remediation efficiency and microbial community composition. This study acted as the first to describe the temporary decommissioning …

The Ethical Justification Of Extracorporeal Interval Support For Organ Retrieval (Eisor) Within The Context Of Donation After Circulatory Determination Of Death (Dcdd), Jude Anyaeche

Electronic Theses and Dissertations

THE ETHICAL JUSTIFICATION OF EXTRACORPOREAL INTERVAL SUPPORT FOR ORGAN RETRIEVAL (EISOR) WITHIN THE CONTEXT OF DONATION AFTER CIRCULATORY DETERMINATION OF DEATH (DCDD)

ABSTRACT

Donation after Circulatory Determination of Death (DCDD) protocol contributes to the increase in the number of organs available for transplant. However, the primary practical limitation of the DCDD practice is that the retrieved organs are not of good quality. The organs retain long periods of warm ischemia between the cardio-circulatory arrest of donors and the cold preservation of the donated organs. Within the period, ischemic damage affects the organs, leading to the graft's primary non-function or delayed-graft …

Natural Law And Latinx Social Ethics: A Narrative Approach, Mathew D. Scruggs

Electronic Theses and Dissertations

This dissertation will argue concepts rooted in Latinx social ethics provides a corrective to acontextual and ahistorical, Euroamerican natural law frameworks by positing natural law as a type of moral reasoning rooted in the contexts, histories, and narratives of particular communities. Rooting natural law in the narrative practices of Latinx communities not only reveals the need for justice for oppression against Latinx bodies from the wider community, but also offers a corrective to ahistorical and acontextual Euroamerican natural law frameworks. A Latinx natural law functions as a radical and prophetic critique against systems of oppression that destroy the lives of …

Development Of A Habitat Model For An Endemic Leuciscid, The Sandhills Chub (Semotilus Lumbee), Garrett Mitchell Herigan

Electronic Theses and Dissertations

No abstract provided.