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Algorithms For Haplotype Inference And Block Partitioning, Satya Ravi Vijaya

Electronic Theses and Dissertations

The completion of the human genome project in 2003 paved the way for studies to better understand and catalog variation in the human genome. The International HapMap Project was started in 2002 with the aim of identifying genetic variation in the human genome and studying the distribution of genetic variation across populations of individuals. The information collected by the HapMap project will enable researchers in associating genetic variations with phenotypic variations. Single Nucleotide Polymorphisms (SNPs) are loci in the genome where two individuals differ in a single base. It is estimated that there are approximately ten million SNPs in the …

An Investigation Of The Chemistry And Antimalarial Activity Of The Marine Soft Coral Asterospicularia Randalli, S. Duane Burt

Electronic Theses and Dissertations

No abstract provided.

Epidemiological Models For Mutating Pathogens With Temporary Immunity, Neeta Singh

Electronic Theses and Dissertations

Significant progress has been made in understanding different scenarios for disease transmissions and behavior of epidemics in recent years. A considerable amount of work has been done in modeling the dynamics of diseases by systems of ordinary differential equations. But there are very few mathematical models that deal with the genetic mutations of a pathogen. In-fact, not much has been done to model the dynamics of mutations of pathogen explaining its effort to escape the host's immune defense system after it has infected the host. In this dissertation we develop an SIR model with variable infection age for the transmission …

Mechanisms Of The Persistence Of Antibiotic Resistant Enteric Bacteria In Freshwater Wetland Environments, Andrekeus Lee

Electronic Theses and Dissertations

No abstract provided.

Molecular And Biochemical Changes In The Ileum Of Weanling Pigs Fed Antibiotics As Growth Promotant And In Germ-Free Conditions, Sajan George

Electronic Theses and Dissertations

Antibiotics are routinely fed as growth promoters to weanling pigs for the past 50 years. However, frequent use of feed antibiotics potentially results in emergence of drug resistance in microbes, which could be transferred to human pathogens and would interfere with the effective use of therapeutic antimicrobials in human medicine. Such health concerns about the use of antibiotics in animal feeds have led to considerable scientific research and debates. Recently, use of antibiotics in animal feeds has been banned in the European Union and would possibly impact US agriculture soon. This study was conducted to understand the molecular mechanisms of …

Primary Care Nurse Attitudes, Beliefs And Confidence Levels Regarding Alcohol Abuse And Its Treatment: Impact Of Educational Intervention., Raja Sekhar Vadlamudi

Electronic Theses and Dissertations

Alcohol abuse has been a major burden on the society. In the fight against it a key issue the education of the primary care nurses has been ignored. This study evaluates the effect of education program on the attitudes, beliefs, and confidence levels of primary care nurses regarding alcohol abuse and its treatment. Data from the Project Mainstream educational intervention were used with permission from the investigators. Two hundred one students and faculty of nursing at Vanderbilt University participated in the intervention, which was designed to train primary care providers in the Brief Negotiated Intervention technique for early detection and …

Real Time Rt-Pcr For Direct Detection Of Viable Mycobacterium Avium Subspecies Paratuberculosis In Chron's Disease Patients And Association Of Map Infection With Downregulation In Interferon-Gamma Receptor (Infg1) Gene In Crohn's Disease Patients, Mounir Chehtane

Electronic Theses and Dissertations

Association of Mycobacterium avium subspecies paratuberculosis (MAP) with Crohn's disease (CD) and not with ulcerative colitis (UC), two forms of inflammatory bowel disease (IBD), has been vigorously debated in recent years. This theory has been strengthened by recent culture of MAP from breast milk, intestinal tissue and Blood from patients with active Crohn's disease. Culture of MAP from clinical samples remained challenging due to the fastidious nature of MAP including its lack of cell wall in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase …

The Influences Of System Affiliation, Size, And Location On Bioterrorism Preparedness Among Florida Hospitals, Kourtney Scharoun

Electronic Theses and Dissertations

In the event of a bioterrorist attack, emergency departments are considered the first line of response for all acute levels of care. This study focused on hospital emergency departments in Florida and the activities, policies, and procedures involved in preparing for a bioterrorist attack. Hospital size, location, and system affiliation were related to attaining these levels, and their impact was assessed. Through a cross-sectional survey design, the physical properties of the 77 hospitals (i.e. facilities, equipment, communication systems, etc.), and the social characteristics of the organizations (managerial functions including: planning, training, financial, and environmental characteristics) were examined. One-way analysis of …

Hiv/Aids Education In The Public High Schools Of Three Counties In Southwestern Pennsylvania, Timothy Sheets

Electronic Theses and Dissertations

Infection by HIV, the virus that causes AIDS, continues to grow worldwide. The only way to stop infection is to educate the population on prevention by identifying methods of transmission and behaviors that put a person most at risk. Schools are an effective place to teach young people about HIV. The Pennsylvania School Code requires that HIV/AIDS education be provided to all public school students while details of the instruction are locally determined. This study investigated HIV/AIDS education in three counties of southwestern Pennsylvania: what was included in the curricula, what was taught to the students, and how the content …

Part I : Isolation Of Dipentyl 2-(4-(Pentan-3-Yl) Phenyl) Malonate From Sanguisorba Officinalis Labill Part Ii: Synthesis Of A Novel Family Of Ethers Of Podocarpic Acid, Manjeera Yalavarty

Electronic Theses and Dissertations

The goal of part I of this thesis was to isolate pure anti cancer compounds from the Russian plant Sanguisorba officinalis. This plant was selected for investigation because it showed 100% activity against leukemia (L1210 mouse leukemia cells) during the preliminary screening of approximately 100 Russian plant extracts. This work has resulted in the isolation of novel compound 11 using traditional chromatography techniques. Compound 11 was characterized with spectroscopic techniques such as IR, 1H NMR, 13C NMR, DEPT, DQCOSY and MS. Compound 11 was assigned the structure dipentyl 2-(4-(pentan-3-yl) phenyl) malonate. The goal of part II of this thesis was …

Beyond The Death Of The Subject : Suicide In The Works Of Dostoevsky, Camus And Hemingway, William Jared Hogue

Electronic Theses and Dissertations

No abstract provided.

Confocal Laser Scanning Microscopy As A Tool For The Investigation Of Tetracycline Fluorescence In Archaeologicalhuman Bone, Corey Maggiano

Electronic Theses and Dissertations

Fluorochromes such as tetracycline have been used to label bone for histomorphometric analysis, measuring bone formation, growth, maintenance, and pathology. More recently, similar fluorescence has been observed in ancient human bone. Attributed to tetracycline (TC) exposure, this phenomenon could affect various aspects of health during life and/or preservation of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate analysis. Confocal Laser Scanning Microscopy (CLSM) has been demonstrated as a valuable …

Structural Study Of Disease Related Proteins, Ayse Sinem Ozyurt

Electronic Theses and Dissertations

The availability of complete sequences for the genomes of many organisms changes the researcher's attention from genome sequencing to finding the function of gene products, the proteins. The three dimensional (3D) structure of a protein is helpful to understand its function, because tertiary structure is more conserved in evolution than sequence. The 3D structures can be found quickly by using high-throughput (HT) methods for protein expression, purification, characterization and structure determination. This process is very challenging in every step. Therefore we selected nearly 200 cancer related proteins as targets to see the difficulties in trying to determine the web predicted …

Protestant's Guide To Catholic "Nunsense": A Performance Study And Analysis Of "Sister Mary Hubert", Anita Endsley

Electronic Theses and Dissertations

Anita Endsley will portray the role of Sister Mary Hubert in the musical Nunsense at Seaside Music Theatre in Daytona Beach, Florida from October 28 through November 14, 2005. This parody of convent life in crisis written by Dan Goggin provides a breadth of material for analysis of the genre as well as character analysis. Dissecting the comedy will be the foundation for characteer development. The popularity of this musical among Catholic and non-Catholic audiences have created a market that inspired the writer to extend this theme through five sequels. The commercial success of these musicals suggest a strong connection …

Non-Specific Stress Indicators And Growth Efficiency In The Archaic And Mississippian At The Perry Site (1 Lu25) In Northwestern Alabama, Robin Wiebe

Electronic Theses and Dissertations

Porotic hyperostosis, linear enamel hypoplasias and adult stature are all nonspecific indicators of stress. When studied together, they provide an understanding of the long-term survival of periods of stress occurring in varying stages of the life of an individual. Because the Perry Site contains both Archaic and Mississippian components, it provides a unique opportunity to examine not only how these indicators interact, but also how interactions may change across time and subsistence base. Most studies show increases in frequency of porotic hyperostosis and linear enamel hypoplasias and a decrease in adult stature with a shift to agriculture. The Perry material …

Validity Of Self-Reported Data On Seat Belt Use: The Behavioral Risk Factor Surveillance System., Agnes Mary Banks Samples

Electronic Theses and Dissertations

Personal lifestyle and behavior are associated with the 10 leading causes of death for Americans. Motor vehicle crashes kill more than 40,000 people and injure more than 3 million people annually in the United States, representing one of America's most serious health and economic problems. According to the National Highway Traffic Safety Administration (NHTSA), someone in America is injured in a motor vehicle crash every 14 seconds and someone is killed every 12 minutes (as cited in Ad Council, 2003). It is widely accepted that increased use of safety belts and reductions in driving while impaired are two of the …

The Impact Of A Rural School-Based Health Center On Students And Their Families In Sneedville, Tennessee: A Case Study., Michael D. Belcher

Electronic Theses and Dissertations

The purpose of this study was to describe the impact that the school-based health center in Sneedville, Tennessee had on students who attended Hancock County High School. The study documented how the school-based health center affected students, families, and the community of Sneedville. Case study methodology was used to determine if the school-based health center impacted school attendance, mental and emotional health, sexual practices, and overall quality of life for students and their families.

Data were collected through 25 interviews with health center stakeholders. The methodology included working with the staff at the school-based health center to identify the key …

Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams

Electronic Theses and Dissertations

The role of Mycobacterium avium subsp paratuberculosis (MAP) in the etiology and pathogenesis of inflammatory bowel disease (IBD) including Crohn's Disease (CD), has been investigated. The fastidious characteristics and cross reactivity of MAP with other members in Mycobacteria have produced significant challenges in their detection and identification. In this two year pilot study, an array of three PCR molecular assays based on the detection of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. …

The Impact Of Traditional Shona Beliefs On Hiv/Aids Intervention Work In Zimbabwe, Brian Goercke

Electronic Theses and Dissertations

This study examined the relationship between secondary students in the rural and peri-urban areas of Mashonaland in Zimbabwe and their tendencies to agree or disagree with traditional Shona attitudes, beliefs and practices as they related to the HIV/AIDS pandemic. A convenience, cluster sample was used to identify the 482 students who participated in this study. A questionnaire composed of demographics, hypothetical and open-ended questions was administered to these students within the regular classrooms of their respective schools.

A notable finding in this study was that traditional Shona beliefs appear to be stronger in the rural areas. However, the students surveyed …

Effects Of Fire On The Nutritional Ecology Of Selected Ungulates In The Southern Black Hills, South Dakota, Teresa J. Zimmerman

Electronic Theses and Dissertations

Research in the 1990s indicated that populations of white-tailed deer (Odocoileus virginianus) in the northern and central Black Hills had declined since the late 1970s likely due to habitat deterioration. In August 2000, the Jasper fire consumed nearly 35,000 ha of ponderosa pine (Pinus ponderosa) forest in the southern Black Hills. To ascertain the nutritional condition of white-tailed deer and mule deer (O. hemionus) and their response to fire, 5 adult white-tailed deer and 5 adult mule deer were collected in burned and unburned habitat, in February and August, 2002 and 2003. Collected deer were weighed and necropsied at a …

Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya

Electronic Theses and Dissertations

Anthrax, a fatal bacterial infection is caused by Bacillus anthracis, a gram-positive, spore forming, capsulated, rod shaped organism. Centers for Disease Control (CDC) lists anthrax as Category A biological agent due to its severity of impact on human health, high mortality rate, acuteness of the disease and potential for delivery as a biological weapon. The currently available human vaccine in the United States (AVA anthrax vaccine adsorbed) is prepared from Alum adsorbed formalin treated supernatant culture of toxigenic, non-encapsulated strain of Bacillus anthracis with the principle component being protective antigen (PA83). Evaluation of anthrax vaccine given to nearly 400,000 US …

An Investigation Of The Impact Of Sandplay Therapy On Mental Health Status And Resiliency Attitudes In Mexican Farmworker Women, Ximena Elizabeth Mejia

Electronic Theses and Dissertations

This study investigated the impact that sandplay therapy sessions had on Mexican farmworker women's mental health status and resilience attitudes. The participants of this study were 40 women who were born in various states in Mexico and presently reside in rural Central Florida. Twenty women participated in the control group and twenty women participated in the treatment group. The impact sandplay therapy has on participants' mental health status was measured by Outcome Questionnaire (OQ-45), which assesses progress in therapy; and Resiliency Attitudes Scale (R.A.S.), which determines resiliency attitudes. The study also includes twenty participants' transcriptions of their individual sessions to …

A Study Of Why Older People 65+ Do Or Do Not Exercise, Nancy Mccoin Williams

Electronic Theses and Dissertations

This qualitative study uses interviews to examine factors explaining why 20 elderly individuals from a variety of occupations and still living in the community independently did or did not exercise. Within the limits of this qualitative study, the reasons elderly participants gave for avoiding exercise included fear of injury, joint pain, poor health, complications from various degenerative diseases, and a lack of enjoyment of exercise. The reasons for exercising included necessity due to post-heart attack motivation, work-site exercise program or equipment availability, opportunity to habituate work-related tasks, spouse support, history of exercise experience, ability to exercise without injury, enjoyment of …

One Bug, Two Drugs : A Mathematical Model Of Resistance Dynamics In The Icu., Susan L. Calcote White 1971-

Electronic Theses and Dissertations

Antibiotic resistance is a problem causing growing concern in the medical community, leading some to speculate that a return to the preantibiotic era is imminent. The problem of antibiotic resistance is particularly significant in the intensive care unit (ICU), due to the weakened immune responses of the patients and quantity of antibiotics administered. One theory proposes that the policy of cycling, or rotating, the antibiotics used in the ICU may minimize the development of resistance. Few clinical trials investigating the effects of cycling have been conducted, and many questions concerning the impact of cycling policies are unanswered at this point. …

Health Practices Of Homeless Women, Margaret Chaney Wilson

Electronic Theses and Dissertations

Homeless women and female-headed families represent the fastest growing subgroups of homeless individuals. To expand the body of knowledge and provide further insight into the complex area of homelessness and health, health practices of homeless women were investigated using a cross-sectional, descriptive, and non-experimental design using Pender's Health Promotion Model as the theoretical framework. Homeless women (N=137) were recruited from five shelters in northeastern Indiana. Homeless women in this study were found to be highly educated, mostly unemployed, and primarily single. A greater number of African Americans than represented in the local population were found to be shelter residents. Health …

Stochastic Modeling Of Nonlinear Epidemiology, Sankar Bokka

Electronic Theses and Dissertations

No abstract provided.

The Making Of The Homoerotic Body : Southern Evangelicals And The Fight For Gay And Lesbian Rights, Courtney E. Chartier

Electronic Theses and Dissertations

No abstract provided.

Bifurcation Analysis Of Hiv-1 Dynamics In Vivo, Revanth Babu Mutyala

Electronic Theses and Dissertations

No abstract provided.

Folk Medicine In Southern Appalachian Fiction., Catherine Benson Strain

Electronic Theses and Dissertations

The region of Southern Appalachia, long known for its colorful storytellers, is also rich in folk medical lore and practice. In their Appalachian novels, Lucy Furman, Emma Bell Miles, Mildred Haun, Catherine Marshall, Harriette Arnow, Lee Smith, and Charles Frazier, feature folk medicine prominently in their narratives. The novels studied, set against the backdrop of the rise of official medicine, are divided into three major time periods that correspond to important chapters in the history of American medicine: the 1890s through the 1930s; the 1940s through the 1960s; and the 1970s through the present. The study of folk medicine, a …

Structure-Function Studies Of The Vaccinia Virus Complement Control Protein., Scott Alan Smith

Electronic Theses and Dissertations

The vaccinia virus complement control protein (VCP) is involved in the modulation of the host inflammatory response during vaccinia virus infection. It possesses the ability to inhibit both classical and alternative pathways of complement activation, as well as bind to heparin or heparan sulfate proteoglycans, making it a unique multifunctional protein with therapeutic potential. VCP is able to block complement activity through its ability to bind C3b and C4b. Other very novel activities arise from VCP's ability to bind heparin and heparan sulfate proteoglycans, allowing the protein to attach itself to cell surfaces. The goal of this research was to …