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Comparison Of The 2022 Monkeypox (Mpox) Outbreak Using Mathematical Modeling And Time Series Clustering, Mark-Daniels Tamakloe

Electronic Theses and Dissertations

Monkeypox virus (MPXV) is the causative agent of monkeypox (mpox), a rare viral disease that affects humans [1]. It is primarily found in Africa and is transmitted to humans through contact with sick animals, particularly rodents and monkeys, or through human-to-human transmission [2]. From the beginning of May 2022, cases of mpox have been recorded from non-endemic nations, and the illness has continued to be reported in other endemic nations. Majority of confirmed cases have been recorded in Europe and North America. In this thesis, we compare the spread of the outbreak across the top ten countries using a combination …

Extraction, Purification, And Characterization Of Potential Bioactive Compounds Produced By Janthinobacterium Lividum Tajx1901, Andy Elorm Agbakpo

Electronic Theses and Dissertations

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel compounds. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). The isolated soil bacterium was successfully cultured on rich media agar plates, followed by extraction using methanol and chloroform. The purification methods utilized include flash column chromatography, preparative thin-layer chromatography, and …

Microsporidian Spores And The Integrin Binding Loop Of The Madam Protein Are Important For Integrin Signaling And Attachment To Host Cells, Cindy L. Barrett

Electronic Theses and Dissertations

Microsporidia are a distant fungal pathogen that have severe clinical consequences for the immunocompromised. Previous work identified a microsporidian pathogen protein termed Microsporidian ADAM or MADAM. This protein has close sequence homology to other ADAM proteins (A Disintegrin and Metalloproteinase) in two microsporidian species, Encephalitozoon intestinalis and E. cuniculi. ADAM proteins have a wide range of functions, including binding to integrins and host signaling. It is known that many pathogens manipulate integrins to invade host cells, and it is predicted that microsporidia are also exploiting this host target. Previous work with the MADAM protein demonstrated that this protein has a …

Overdose Prevention Behaviors And The Rural Risk Environment Among People Who Inject Drugs In Rural Appalachia, Jody Teel

Electronic Theses and Dissertations

Introduction: The entire United States has felt the effects of the overdose epidemic, but rural Appalachia has experienced disproportionate overdose deaths. This variation in overdose mortality can be attributed to the risk environment for overdose in rural Appalachia. Overdose prevention behaviors are individual level behaviors that people who inject drugs can implement that may have the potential to reduce their risk of overdose, however limited research exists regarding the utilization of those behaviors. It is important to consider how the risk environment can influence the utilization of overdose prevention behaviors among people who inject drugs. Therefore, the purpose of this …

Met Alterations In Glioblastoma: Characterization Of Patient-Derived Xenografts And Therapeutic Strategies, Anna Musket

Electronic Theses and Dissertations

Glioblastoma is the most commonly diagnosed central nervous system primary malignancy and it is considered a terminal diagnosis with few treatment options available. Glioblastoma tumors frequently develop treatment resistance due in part to their highly heterogenic nature. The heterogeneity of glioblastoma is partially attributed to the presence of glioma stem-like cells (GSC), which are highly invasive and resistant to chemotherapy and irradiation treatments. Signaling of the receptor tyrosine kinase MET is a known regulator of GSC. Glioblastoma patients have an increasingly poor prognosis that corresponds with increasing MET expression. Both GSC and MET are known to contribute to treatment resistance …

Icu Nurses’ Adherence To The Cdc’S Central Line Bundle: A Qualitative Study, Cherie Burke, Cherie Burke

Electronic Theses and Dissertations

Background: Central line associated bloodstream infections (CLABSIs) are one of the most common healthcare associated infections and result in prolonged hospital stays, significant morbidity, and increased costs to the healthcare system. Adherence to the CDC central line maintenance bundle has been shown to be effective in decreasing CLABSI yet these infections continue to be a problem. ICU nurses play a vital role in managing central lines and adhering to the maintenance guidelines, however different situations may arise during the care that may impact CLABSIs.

Objectives: 1) To determine nurses’ adherence to the CDC’s maintenance central line

bundle; 2) To determine …

Identification Of A Role Beyond Iron Acquisition For Yersiniabactin During Yersinia Pestis Infection., Sarah Price

Electronic Theses and Dissertations

Yersinia pestis is a Gram-negative re-emerging bacterial pathogen that is responsible for bubonic, septicemic, and pneumonic plague. Y. pestis and other bacteria require transition metals, such as iron, zinc, and manganese, to maintain intermediary metabolism, transcriptional regulation, and virulence. To inhibit infection, eukaryotic organisms have developed distinct mechanisms, called nutritional immunity, to sequester these important nutrients from invading bacteria. For pathogens to colonize the vertebrate host, they have evolved dedicated acquisition systems for transition metals. During infection, Y. pestis overcomes iron limitation by secreting the siderophore yersiniabactin. Additionally, Y. pestis requires zinc for infection and utilizes high affinity transporters to …

Our Story, Our Song: Assessing And Addressing Black Women's Healthcare Experiences., Jason Deakings

Electronic Theses and Dissertations

Despite advancements in medicine and health sciences, health disparities and declining life expectancy persists among Black women in the United States. Researchers and non-traditional healthcare providers suggest alternative healing (e.g., music and Sister Circles) and research methodologies to achieve a culturally conscious, traumainformed, quality, and holistic care and practice. The intersecting identities and cultural responsibility of Black women highly influences the barriers and facilitators on the uptake of healthcare services. Utilizing a community engaged research approach, this qualitative study assessed the healthcare experiences, as well as the role of music in the lived and retold experiences of Black women navigating …

Transmission And The Evolution Of Diseases Caused By Chlamydia Trachomatis, Sars-Cov-2, And Plasmodium Species., Nathan Steffens

Electronic Theses and Dissertations

Principles of natural selection have proven valuable for explaining why pathogens cause the diseases that they do. In theory, the evolved level of host exploitation should reflect how dependent a pathogen is on host health for transmission. This dependency is shaped by transmission mode and transmission opportunity, which should therefore be predictors of disease manifestations. In this dissertation, I apply these principles to investigations of depression in Chlamydia trachomatis and virulence of SARS-COV-2 and Plasmodium species. This dissertation has five chapters. In chapter I, I describe the theoretical foundation of my dissertation research. I also briefly introduce each study system. …

Building Resilience: A Mixed Methods Exploration Of Head Start Teachers Coping During The Covid-19 Pandemic, Ehichoya Edokhamhen

Electronic Theses and Dissertations

The purpose of this study was to explore the resiliency of Head Start teachers during the COVID-19 pandemic. The study explored the role social support (as an external factor of resilience) and self-efficacy (as an internal factor of resilience) played in the resilience of Head Start teachers during the COVID-19 pandemic. This study employed a sequential explanatory mixed methods design involving two phases: a quantitative and a qualitative phase. Participants in this study were 99 teachers in rural eastern Tennessee and some rural parts of Virginia, North Carolina, and New Mexico, who were Head Start teachers during the COVID-19 pandemic. …

Covid-19 & Mathematics Achievement: A Casual-Comparative Study, Jay M. Johnson

Electronic Theses and Dissertations

Learning loss due to COVID-19 and the digital divide will have dire consequences for low-income students. This study used the Faucet Theory (Alexander et al., 2001) as a theoretical framework to determine the extent that the COVID-19 learning environment impacted the Southern Public Schools District’s African American, low-income, and high-income high school students’ proficiency level based on the Algebra I section of the 2020-2021 Louisiana Education Assessment Program (LEAP) assessment. The researcher used archival data from the LEAP, a causal-comparative research design, and a one-way ANOVA to test the hypotheses and answer the research question. The results of the one-way …

Cardiovascular Disease In Central Appalachia, An Exploratory Study Of Behavioral, Community, And Patient-Centered Care Influences, Kristy Gagnon

Electronic Theses and Dissertations

The Central Appalachian region of the United States disproportionately experiences higher rates of cardiovascular diseases (CVD) and associated risk factors. Primary risk factors for CVD include hypertension, hypercholesterolemia, and tobacco use, all of which have the potential to be mitigated through lifestyle behavior changes. Dietary and physical activity practices are the two main driving forces for the development of hypertension and hypercholesterolemia, while tobacco use is a behavioral choice on its own. Subsequentially, shifting lifestyle choices to emphasize healthier living has the potential to reduce CVDs within the Central Appalachian region. Considering behavioral choices are not made in isolation and …

Message Framing For Covid-19 Vaccine Uptake: A Content Analysis Of Cdc Facebook Communication, Victoria Amoako

Electronic Theses and Dissertations

The study examined the frequent frames adopted by the CDC in the communication of the Covid-19 vaccine and how it impacts vaccine uptake through quantitative content analysis. The study builds on the framing theory and elaboration likelihood model. This study’s content collected 327 posts by the CDC on covid-19 vaccinations from December 2020- December 2022. A research randomizer was used to choose 200 posts as the final sample from the data that had been gathered. 122 posts out of the 200 total posts that the randomizer tallied were pertinent to the research questions for the study. It was found that …

Emerging Tick-Borne Diseases In Northeast Tennessee, Jacob Schultz

Electronic Theses and Dissertations

Tick populations have been immigrating into northeast Tennessee from east Tennessee, Kentucky, Virginia, and North Carolina. Counties in states bordering northeast Tennessee harbor tick species associated with human illness. Human diseases transmitted by ticks include ehrlichiosis, spotted fever rickettsial group diseases, tularemia, anaplasmosis, babesiosis, Lyme disease, alpha-gal syndrome, Heartland virus, Powassan virus, and southern tick-associated rash illness (STARI). These diseases cause morbidity and mortality in human populations and may pose a high risk to individuals, wildlife, and livestock. The Cherokee National Forest covering the east Tennessee border provides a permissible environment for ticks to immigrate and thrive. Residents of northeast …

Comparative Characteristics Of Integrin Αdβ2 Binding To Native Fibrinogen And Fibrinogen Modified By Dha Oxidation During Inflammation, Ajibola Ilesanmi

Electronic Theses and Dissertations

2-ω-carboxyethylpyrrole (CEP) is a product of docosahexaenoic acid (DHA) oxidation, which forms covalent adducts with different proteins. CEP-modified proteins can interact with macrophage receptor, integrin αDβ2. This study aims to compare αDβ2 binding to its physiological ligand, fibrinogen, and CEP-modified fibrinogen, which is formed during inflammation. We hypothesize that modification of fibrinogen changes its ligand-binding properties to integrin αDβ2 which can affect macrophage migration and retention. Recombinant αD I-domain and αDβ2-transfected HEK293 cells were used for the experiments. Using biolayer interferometry, we found that the affinity of αD I-domain binding to fibrinogen-CEP was higher than fibrinogen and inhibited by the …

Cyclic-Di-Gmp Regulates Salmonella Typhimurium Infection Of Epithelial Cells And Macrophages, Abdulafiz Musa

Electronic Theses and Dissertations

Regulation of the bacterial second messenger cyclic-di-GMP in Salmonella Typhimurium allows it to delicately alter phenotypes to optimize invasion and survive intracellularly in epithelial cells and macrophages to become virulent and cause infection. Cyclic-di-GMP concentration is regulated by the presence of external stimuli, sensory diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), and cyclic-di-GMP binding effectors. Previous studies established that maintenance of low cyclic-di-GMP concentrations is required for survival in macrophages, and that deletion of 3 active PDEs reduces this survival. Here I showed that these 3 PDEs also influenced the infection of epithelial cells. Further studies re-established the decreased survival in …

Reducing Readmission Rates Among Patients Who Have A Dual Diagnosis Of Both Mental And Physical Illnesses, Hannah M. Shahan

Electronic Theses and Dissertations

Readmission rates are a primary indicator used by acute care hospitals to determine the effectiveness of healthcare treatments and interventions. High readmission rates indicate the existence of a gap in the continuum of care. Acute care hospitals focus on the treatment of the immediately urgent physical health condition. Once the immediate presenting illness is treated or stabilized, the patient is discharged. This focus largely leaves mental illness undiagnosed and untreated. However, chronic, untreated mental illnesses are often comorbid with physical illnesses. In studies comparing patients with comorbid physical and mental illness to patients with only a physical illness, comorbidity was …

Factors That Impact Prevention Therapy For A Person(S) Living With Hiv In A Conservative, West Texas City, Madison E. Reyna

Electronic Theses and Dissertations

Human Immunodeficiency Virus (HIV) is well known for being a sexually transmitted virus that will result in Acquired Immunodeficiency Syndrome (AIDS) if left untreated. Some individuals are unaware of the other risk factors that can result in the transmission of HIV. The transmission of HIV can occur through the spread of HIV-positive blood, oral, anal, and vaginal sex without a condom or protection, sharing injection drug equipment, and perinatal transmission (Centers for Disease Control and Prevention, 2022). Despite advances in Antiretroviral treatments (ART) for HIV, there are still factors that are preventing individuals living with HIV/AIDS to maintain or suppresses …

The Effect Of An American Heart Association–Approved Exercise Regimen On Burnout In Nurse Practitioners And Physician Assistants Working In Emergency Departments, Michelle Lee Johnson

Electronic Theses and Dissertations

Burnout is a widely known state of physical and emotional fatigue previously noted to affect as many as 60% of healthcare providers, but one that is understudied in nurse practitioners and physician assistants working in emergency departments. It is imperative that the providers of healthcare recognize in themselves signs of burnout and have resources available to mitigate the condition. A pretest–posttest, quasi-experimental, nonequivalent control group, descriptive research design with descriptive statistics analysis was used to analyze answers to an email-based survey sent to nurse practitioners and physician assistants working for one large emergency medicine staffing company. The intent of this …

Harness Therapeutic Potential Of Immune Responses: Molecular Characterization And Targeted Delivery, Nan Ji

Electronic Theses and Dissertations

The immune system plays a crucial role in protecting the body from harmful pathogens and cancer cells and maintaining tissue homeostasis. Immunotherapy has shown significant potential in treating various diseases by harnessing the therapeutic potential of immune responses through the targeting of different immune cells, receptors, and signaling pathways. This dissertation investigated two immune modulators for their immune-enhancing potential.In the first chapter, the components of innate immunity are introduced. In the second chapter, Immulina, a commercially available extract of Arthrospira platensis enriched with Braun-type proteins, was studied for its immunostimulatory effect in mouse bone marrow-derived dendritic cells and macrophages. The …

Comparison Of Self-Reported Depression And Anxiety Scores Between U.S. Households With And Without Children At Early And Later Stages Of The Covid-19 Pandemic, Hanan Mohammed Eissa Jamali

Electronic Theses and Dissertations

Parents in particular, are disproportionately affected by the 2019 coronavirus pandemic and the lockdowns that followed. Parents had to find a way to balance work, teaching, and taking care of their kids when schools were forced to close for safety reasons. While changes in parents' mental health have been the subject of a number of studies, there have been no studies comparing the level of depression and anxiety experienced by parents with and without children below the age of 18 years in the United States. Data for this study came from Households Pulse Survey (HPS) (week 2 N = 41,996; …

Development Of Narrow-Spectrum Agents And Mray Inhibitors Targeting Multidrug-Resistant Mycobacterium Tuberculosis, Tomayo Ireti Berida

Electronic Theses and Dissertations

Tuberculosis (TB) is a major global health burden. This airborne infectious disease is caused by Mycobacterium tuberculosis (Mtb) and resulted in 1.6 million deaths globally in 2021. The emergence and spread of multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) underscores the urgent need to discover and develop new agents, particularly those with narrow spectrum activity and those acting by novel mechanisms. Although broad-spectrum antibacterials have been successful in treating drug-resistant bacterial infections, including TB, there is a push toward the discovery and development of anti-TB agents with narrow-spectrum activity. This approach aims to limit the spread of resistance across …

Novel Formulation Strategies For Oral And Ocular Drug Delivery, Samir Senapati

Electronic Theses and Dissertations

Nanoformulations such as nanoemulsions (NE), solid lipid nanoparticles (SLN), and nanostructured lipid carriers (NLCs) are colloidal particulate systems in the submicron size range and act as carriers of drug molecules. Their size varies from 10 to 1,000 nm (1). The capacity of these nanoparticles to permeate through many anatomical barriers, the prolonged release of their contents, and their stability on the nanometer scale have widely contributed to their efficacy in drug delivery. Owing to the advantages of nanoformulations, they were further explored in our studies to target different diseases like Dry eye disease (DED), glaucoma, and malaria.

Chapter 2 discusses …

In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez

Electronic Theses and Dissertations

The transition period is a crucial stage in the lactation cycle and can lead to significant metabolic changes in cows. The use of nutritional interventions, such as direct-fed microbials, may assist cows during the transition from pregnancy to early lactation, reducing the occurrence of metabolic disorders and improving overall health. The primary goal of this study was to assess the effects of a commercial rumen-derived directfed microbial (DFM) product (Galaxis™ Frontier (GF) Native Microbials Inc., CA, USA) on various factors such as performance, blood biomarkers, rumen fermentation, and bacterial population in dairy cows during the transition period up to 100 …

Voices From Below: The Politics Of Leprosy Control In Southeastern Nigeria, 1926-1960, Odinakachukwu Kingsley Eze

Electronic Theses and Dissertations

Leprosy was an endemic disease in colonial Southeastern Nigeria but became an epidemic between the 1920s and 1940s. Unlike malaria and sleeping sickness, its endemicity did not impede European penetration at the dawn of the twentieth century. However, its spread across Nigeria intensified during colonial rule, with acute prevalence in regions hitherto prone to the disease. Therefore, from the 1920s to 1960, Southeastern Nigeria became the epicenter of British policies tailored towards preventing, curing, and eradicating leprosy disease in Nigeria. Existing research has established the involvement of different actors in this course–including Christian missionaries, colonial officials, specialized agencies, and international …

Sociocultural Determinants Of Exclusive Breastfeeding And Infant Feeding Practices In Niger, Maimouna Issoufou Kapran

Electronic Theses and Dissertations

Niger is one of the countries with the lowest exclusive breastfeeding (EBF) rates in the world. The purpose of this study was to determine the barriers to EBF in Niger and to determine the types of feedings that are most commonly practiced in Niger. Participants (n=31) were mothers with children aged 0 to 24 months, recruited on-site at a healthcare facility in Niamey, the capital of Niger. They completed a 20-item questionnaire on socio-demographics, breastfeeding support and knowledge, a short food security survey, and a 24-hour diet recall. All mothers interviewed breastfed at some point, and a majority of the …

Immulina® As An Immunostimulatory Supplement: Dissolution And Pharmacodynamic Studies, Yusheng Li

Electronic Theses and Dissertations

As one of popular dietary supplements, the health benefits of Arthrospira platensis (commonly known as spirulina) have been broadly reported. Immulina is a commercially available extract of Arthrospira platensis that is enriched with bacterial lipoproteins and acts as a Toll-like receptor 2 (TLR2) agonist. However, the immunostimulatory effect of Immulina in vivo has not been well understood. In this work, we explored the nanoformulation of Immulina to improve the dissolution rate in aqueous media. Building upon a standardized cell-based assay that measures the bioactivity of Immulina as a TLR2 agonist, we developed a quantitative methodology to determine the Immulina concentration …

Experimental Studies On Developing And Characterising Vaginal Films Using Hot-Melt Extrusion Technology, Kirti Pradeep Sawant

Electronic Theses and Dissertations

Vaginal Candidiasis (VC) is one of the most common health problems in women. Indeed, one of the most convenient approaches for its treatment is an appropriate mucoadhesive vaginal film. This approach provides a local treatment modality, which inevitably decreases the dosing frequency and improves patient compliance. Clotrimazole is one of the drugs that are extensively used to treat Vaginal candidiasis and therefore, it is used in this research. The current study aimed to produce a novel extended-release film using Hot-Melt Extrusion (HME). Two different polyethylene oxide (PEO) polymer grades (PEO N10, PEO N750) were utilized to optimize the drug release …

Understanding Human Gut Bacterial Interactions And Their Role In Colonization Resistance Against Clostridioides Difficile Using Mini-Bioreactor Array And Caenorhabditis Elegans As Model Systems, Achuthan Ambat

Electronic Theses and Dissertations

No abstract provided.

Identification And Quantification Of Novel Antimicrobials, Hazera Khatun Koly

Electronic Theses and Dissertations

No abstract provided.