Digital Commons Network^™

Entrepreneurial Decision-Making Under Risk : Prospect Theory And Dual-Process Theory., Dalong Ma

Electronic Theses and Dissertations

This research addresses the question of why some people become entrepreneurs whereas others do not. The debate has been going on for decades in entrepreneurship. In this dissertation, I address this question by decomposing it into two related questions. The first question is whether entrepreneurs make different decisions compared to non-entrepreneurs when they are facing the same opportunities under risk. The second question is whether these differences in decision-making (if any) are due to the natural proclivity of entrepreneurs themselves. To identify the differences of entrepreneurial decision-making between entrepreneurs and non-entrepreneurs, this study investigates the nexus between entrepreneurs and opportunities …

Bdnf Maintains Adult Taste Innervation And Is Required For Taste Nerve Regeneration After Injury., Lingbin Meng

Electronic Theses and Dissertations

Brain derived neurotropic factor (BDNF) is required for the gustatory neuron survival,target innervation, and taste bud maintenance during development. However, whether BDNF has any function in the adult gustatory system in normal conditions or after nerve injury is unclear. To address these issues, I inducibly removed BDNF in all cells expressing BDNF in adult mice. In the experimental animals, Bdnf expression decreased to 5% of control mice in the lingual epithelium and geniculate ganglion (p< 0.01) at both two weeks and ten weeks after tamoxifen administration. I found no effect on taste bud morphology at four weeks following Bdnf gene deletion. However, ten weeks following Bdnf gene deletion, P2X3-positive and TUJ1-positive gustatory innervation to individual taste buds was reduced by nearly half (each with p < 0.01) and both taste bud volume and taste cell number decreased 30% (each with p< 0.01). These experiments demonstrate that BDNF is required for maintenance of normal levels of taste innervation and normal taste bud morphology in adulthood. In addition, taste cells expressing PLCß2 (phospholipase C ß2), a marker for taste cells that respond to sweet, bitter and umami, did not decrease after Bdnf gene deletion in the adult. Thus, the missing taste cells are of another type. This indicates that taste cell loss is not uniform across the various taste cell types, even if nearly all taste cell types receive the P2X3 and TUJ1 innervation. Since BDNF is required for initial innervation of the taste system and supports taste bud innervation and size in adulthood, it could also be required for nerve reinnervation after injury. To determine if Bdnf is still expressed following nerve section, the chorda tympani nerve (taste nerve) was sectioned and Bdnf level was detected with Real Time RT-PCR. Bdnf continued to be expressed at normal levels from two days to two months post-surgery in both geniculate ganglion and tongue epithelium. Therefore, BDNF could be involved with chorda tympani regeneration. To determine if this was the case, the Bdnf gene was deleted in adult inducible transgenic mice (under the control of a Ubiquitin promoter) two weeks before chorda tympani nerve section. Taste bud number was reduced by half in all genotypes at two weeks post-surgery (p < 0.01). For the remaining taste buds, gustatory innervation was nearly gone with only a little innervation from the trigeminal nerve remaining in the taste bud (p < 0.01). Taste bud volume (p < 0.01) and taste cell number (p< 0.01) were reduced by half for both control and experimental genotypes. Eight weeks post-surgery, taste bud number recovered in mice without Bdnf gene deletion, but did not recover in mice following Bdnf gene deletion (p < 0.01). Gustatory nerve innervation returned in 70% of the taste buds in control mice (p< 0.01). For those reinnervated taste buds, both taste bud volume and taste cell number increased to normal levels. However, in mice lacking the Bdnf gene, gustatory fibers only reinnervated 7.8% of the taste buds (p < 0.01); for most uninnervated taste buds, both taste bud volume and taste cell number remained small. These experiments demonstrate that BDNF is crucial for promoting regeneration of gustatory nerve fibers in adulthood. Following gustatory nerve section, considerable adult plasticity has been observed on the contralateral side including enlarged taste buds with more cells (Guagliardo and Hill, 2007). To determine if this anatomical change was associated with alter Bdnf expression. I examined Bdnf level in the geniculate ganglion and tongue epithelium on the contralateral side following chorda tympani nerve section. Results showed Bdnf expression increased two fold at two weeks post-surgery in geniculate ganglion on the contralateral side (p < 0.05), indicating BDNF may involve with the observed plastic changes. To determine if the increase in taste bud size was associated with increased innervation and/or regulated by BDNF, the Bdnf gene was then deleted in inducible knockout mice before nerve surgery, and taste bud size and amount of innervation were measured on the contralateral side. The results showed taste bud volume, taste cell number and a marker for nerve fibers all increased on the contralateral side in mice without Bdnf gene deletion at eight weeks post-surgery. This indicates that larger taste buds could be supported by increased TUJ1 positive fibers from trigeminal nerve. In addition, in mice lacking Bdnf, taste bud volume, taste cell number and innervation did not increase on the contralateral side after surgery, which indicates that Bdnf may contribute to larger taste buds on the contralateral side following nerve section by supporting increased innervation to the larger taste buds.

A Novel Diffusion Tensor Imaging-Based Computer-Aided Diagnostic System For Early Diagnosis Of Autism., Mahmoud Mostapha

Electronic Theses and Dissertations

Autism spectrum disorders (ASDs) denote a significant growing public health concern. Currently, one in 68 children has been diagnosed with ASDs in the United States, and most children are diagnosed after the age of four, despite the fact that ASDs can be identified as early as age two. The ultimate goal of this thesis is to develop a computer-aided diagnosis (CAD) system for the accurate and early diagnosis of ASDs using diffusion tensor imaging (DTI). This CAD system consists of three main steps. First, the brain tissues are segmented based on three image descriptors: a visual appearance model that has …

The Second Generation Of The Cccm System For In-Vitro Cardiac Tissue Engineering., Mai-Dung Thi Nguyen

Electronic Theses and Dissertations

Cardiovascular disease is the leading cause of death worldwide. When a myocardial infarction occurs, scar tissue compensates the damaged myocardial tissue. This scar tissue increases the stiffness of the heart tissue, reduces the heart’s function, and finally leads to the heart failure (HF) disease. To have the tissue engraftment, in-vitro cardiac tissue should have the same properties as the native mature cardiac tissue. However, current in-vitro cell culture technologies fail to accurately recreate the in-vivo like mechanically physiological environment for in-vitro cardiac tissue culture, and therefore, fail to regenerate the in-vivo like mature cardiac tissue. Hence, a microfluidic cardiac cell …

Composing College And Career : Mobility, Complexity And Agency At The Nexus Of High School, College And Work., Brice Nordquist

Electronic Theses and Dissertations

This dissertation offers and theorizes findings of a two-year mobile ethnography investigating the complexity of students’ movements within and among secondary and tertiary educational institutions and the labor market. The project illustrates the lateral and recursive natures of students’ educational and occupational trajectories and thereby reveals the mutually constitutive relations among scenes of writing across space and time. While the study follows eleven students moving from different tracks of high school English through their first years at research universities, colleges and full-time jobs, this text focuses specifically on the mobilities of three students: Nadif, Katherine and James. I draw upon …

Mirna-21 Has Anti-Inflammatory Effects In The Macrophage Response To Peritonitis., Rebecca Elise Barnett

Electronic Theses and Dissertations

This project investigated the role of miRNA-21 in the macrophage response to peritonitis; with the goal of understanding whether the associated mechanism has potential benefits in the clinical treatment of peritonitis. A novel therapeutic intervention in the treatment of peritonitis could modulate the host immune response to decrease an exaggerated pro-inflammatory innate response and then prevent or ameliorate the development of sepsis and organ failure. We found the following: 1. MiRNA-21 expression increases in peritoneal macrophages after lipopolysaccharide (LPS) stimulation. 2. Over expression of miRNA-21 decreases TNF-a secretion from macrophages after LPS stimulation. Suppression of miRNA-21 expression results in increased …

Evaluation Of The Safety And Pharmacokinetic Profile Of The Broad Spectrum Antiviral Lectin Griffithsin., Christopher Lynn Barton

Electronic Theses and Dissertations

Carbohydrate binding agents that target viral envelope glycans are being studied for their potential use as microbicides and antiviral therapeutics. Griffithsin is a lectin originally identified in a red alga Griffithisia sp. Multiple studies have shown that GRFT inhibits HIV-1, Coronaviruses, Hepatitis C, influenza and Ebola virus replication in vitro. This antiviral activity suggests potential uses in chemoprophylaxis and disease treatment. However, safety of GRFT administration has not been extensively studied. In vivo testing--chronic subcutaneous treatment as well as single dose subcutaneous, oral, and intravenous administrations of Griffithsin in Sprague Dawley Rats (Rattus Norvegicus)--was used to assess Griffithsin’s pharmacokinetic properties …

An Analysis Of The Potential Effects Of Urbanization On The Water Quality Of The Middle Fork Of Beargrass Creek, Louisville, Kentucky., Olivia Brooks

Electronic Theses and Dissertations

This thesis examined whether water quality diminished as a result of increasing pollution downstream along the Middle Fork of Beargrass Creek, Louisville, Kentucky in response to urbanization. Samples of key water quality indicators/pollutants were collected for 12 weeks (November - January) along the Middle Fork of Beargrass Creek at three different stream gauges. A spatial and statistical analysis was performed to verify any significant correlations between the water quality indicators and pollutants, and whether basin wide landcover/sewer outlet numbers led to an increase in these indicators downstream. Following ANOVA and Kruskal-Wallis statistical testing it was established that turbidity significantly increased …

"My Watch Begins" : Identification And Procedural Rhetoric On Second Screens And Social Networks., K. Shannon Howard

Electronic Theses and Dissertations

Digital rhetoric creates opportunities for examining rhetoric as it evolves daily. This evolution may be described in terms of network circulation and immediate opportunities for publishing and creating. This project analyzes mobile applications and live feeds used during television broadcasts, where rhetoric is closely tied to the work of identifying with another point of view. Producers and designers of dual-screen applications prompt us to answer how we would act if we assumed the role of protagonist and saw the world through her or his eyes. These questions support the idea that identification is not just a relative of empathy or …

Bone Strength And Architecture : Pharmacological Targeting Of Camkk2 As A Method For Enhancing Bone Quality., Zachary James Pritchard

Electronic Theses and Dissertations

Mice lacking or pharmacologically inhibited for calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) have enhanced bone mass and microarchitecture. This enhanced bone mass and architecture is due to changes in osteoblast and osteoclast numbers as well as activity. Whether the enhanced bone mass translated to increased bone quality and strength was further explored by developing a method to directly test the trabecular strength of the distal femur. Micro computed tomography (CT)-based measurement of the length of the epiphysis of the distal femur, aided in its removal, allowing the exposure of the trabecular bone volume used to determine the microarchitecture parameters. Following …

The Powerful Literacy Posse And The Common Core State Standards : A Collection Of Scholarly Papers., Katherine J. Grindon

Electronic Theses and Dissertations

This dissertation is a collection of scholarly papers on the implementation of the English/Language Arts Common Core State Standards (CCSS) and the integration of the standards with critical literacy. The dissertation is divided into five sections, including three papers intended for publication. The first section is the introduction to the dissertation. It explains the context of critical literacy and the CCSS and outlines the study to be conducted. The introduction also explains the three paper format of the collection. The second section explores the integration of critical literacy and the CCSS in one classroom. Through The Advocacy Project, students find …

Genetic And Mechanistic Analysis Of Rat Mammary Cancer Susceptibility., Jennifer Sanders

Electronic Theses and Dissertations

Breast cancer is a complex disease, which is influenced by genetic, epigenetic and environmental components. Genetic susceptibility to breast cancer is made up of high, moderate and low penetrance alleles. High and moderate penetrance alleles are rare and constitute only a small percentage of the genetic susceptibility. Most variation in genetic susceptibility is controlled by low- penetrance, common polymorphisms. Comparative genetics uses model organisms to study human disease. Rat strains exhibit different susceptibility phenotypes to chemical induced carcinogenesis. The Wistar-Furth (WF) rat strain is susceptible to chemically induced mammary carcinogenesis, while the Wistar-Kyoto (WKy) and Copenhagen (COP) rat strains are …

Classification System Of The Physical Streambed Habitat., Abolfazl Shafaie

Electronic Theses and Dissertations

A streambed is the channel bottom of a stream, river, or creek; the physical confine of the normal water flow. It provides necessities for fish and microinvertebrate including shelter, food, and breeding spots. Therefore, it is an essential part of aquatic animal habitats and often seen as an index of stream health. There is no generally accepted method to quantify streambed physical components and depending upon state and organization a variety of methods are used. The procedure provided in the rapid bioassessment protocol (RBP) (Kaufmann et al., 1999; Sylte and Fischenich, 2002) is probably the most widely used method to …

Il-12 Induced Modulation Of Tumor Derived Myeloid Cell Inflammatory Responsiveness., Courtney Jetun Mitchell

Electronic Theses and Dissertations

Tumor infiltrating and tumor associated myeloid cells (TIMs and TAMs) elaborate an array of factors that promote tumor growth and metastasis. IL-12, a potent inflammatory cytokine has been shown to induce regression of many cancers. We hypothesize that IL-12 augments the ability of TIMs and TAMs to respond to inflammatory stimuli providing a window in which these stimuli are more likely to promote tumor destruction. Related to this hypothesis, we asked two questions: (1) Does IL-12 directly change signaling events associated with inflammatory signal transduction? (2) Is IFN? required for the entirety of IL-12 induced enhancement of the response of …

Synthesis Of A Drug Delivery Vehicle For Cancer Treatment Utilizing Dna-Functionalized Gold Nanoparticles., Tyler Brann

Electronic Theses and Dissertations

The treatment of cancer with chemotherapeutic agents has made great strides in the last few decades but still introduces major systemic side effects. The potent drugs needed to kill cancer cells often cause irreparable damage to otherwise healthy organs leading to further morbidity and mortality. A therapy with intrinsic selective properties and/or an inducible activation has the potential to change the way cancer can be treated. Gold nanoparticles (GNPs) are biocompatible and chemically versatile tools that can be readily functionalized to serve as molecular vehicles. The ability of these particles to strongly absorb light with wavelengths in the therapeutic window …

Probiotic Potential Of Bacterial Isolates From ‘Amabere Amaruranu’ Cultured Milk, Blaise B. Boyiri

Electronic Theses and Dissertations

Probiotics are viable nonpathogenic microbes that positively affect host health. Probiotics inhibit infection, activate immunity, and promote mucosal-barrier development. Many microbes have probiotic activity. Nonetheless, the selection of stable strains and their specific mechanism(s) of action are not fully elucidated. Bacteria from ‘Amabere amaruranu’ cultured milk from Kenya were isolated and identified by PCR sequence analysis of the 16S rRNA gene. Isolates were examined for stability to acid and bile, antimicrobial activity, mucin production, and degradation and sensitivity to antibiotics, hence their potential for probiotics. Lactobacillus isolates were acid unstable, bile-stable, nonmucinolytic, and presented antibacterial activity. L. rhamnosus cell fractions …

Characterization Of A Putative Sir2 Like Deacetylase And Its Role In Sabp2 Dependent Salicylic Acid Mediated Pathways In Plant, Md I. Haq

Electronic Theses and Dissertations

Salicylic Acid Binding Protein2 (SABP2) is an enzyme known to play important role in SA mediated pathway. SBIP-428 (SABP2 Interacting Protein-428), a SIR2 like deacetylase, has been found to interact with SABP2. We demonstrate that SBIP-428 functions as a Sirtuin deacetylase. We show that SBIP-428 itself is lysine acetylated. Interactions of a SBIP-428 with SABP2 also raised the possibility of SABP2 itself being lysine acetylated. The recombinant purified SABP2 or native partially purified SABP2 displayed no acetylation. In response to TMV infection, the expression of SBIP-428 was down regulated at 48 hpi. In addition, SBIP-428 was up regulated in plant …

An Agent-Based Model Of Ant Colony Energy And Population Dynamics: Effects Of Temperature And Food Fluctuation, Guo Xiaohui

Electronic Theses and Dissertations

The ant colony, known as a self-organized system, can adapt to the environment by a series of negative and positive feedbacks. There is still a lack of mechanistic understanding of how the factors, such as temperature and food, coordinate the labor of ants. According to the Metabolic Theory of Ecology (MTE), the metabolic rate could control ecological process at all levels. To analyze self-organized process of ant colony, we constructed an agent-based model to simulate the energy and population dynamics of ant colony. After parameterizing the model, we ran 20 parallel simulations for each experiment and parameter sweeps to find …

The Effects Of Working Memory On Brain-Computer Interface Performance, Samantha A. Sprague

Electronic Theses and Dissertations

Amyotrophic lateral sclerosis and other neurodegenerative disorders can cause individuals to lose control of their muscles until they are unable to move or communicate. The development of brain-computer interface (BCI) technology has provided these individuals with an alternative method of communication that does not require muscle movement. Recent research has shown the impact psychological factors have on BCI performance and has highlighted the need for further research. Working memory is one psychological factor that could influence BCI performance. The purpose of the present study is to evaluate the relationship between working memory and brain-computer interface performance. The results indicate that …

Production Of An Antibiotic-Like Activity By Streptomyces Sp. Couk1 Under Different Growth Conditions, Olaitan G. Akintunde

Electronic Theses and Dissertations

Streptomyces are known to produce a large variety of antibiotics and other bioactive compounds with remarkable industrial importance. Streptomyces sp. COUK1 was found as a contaminant on a plate in which Rhodococcus erythropolis was used as a test strain in a disk diffusion assay and produced a zone of inhibition against the cultured R. erythropolis. The identity of the contaminant was confirmed as Streptomyces through 16S rRNA sequencing. This Streptomyces produces a strong inhibitory compound in different growth media. A culture extract from inorganic salts starch agar was found to be very active; producing a large zone of inhibition …

Northern Saw-Whet Owl (Aegolius Acadicus) Abundance And Distribution In The Southern Appalachian Mountains Of Northeast Tennessee, John P. Mccormick

Electronic Theses and Dissertations

Little is known about the distribution, abundance, or life histories of the Northern Saw-whet Owl (Aegolius acadicus) in the Southern Appalachian Mountains of Northeast Tennessee. This study relied upon the Pennsylvania Protocol of audio playback of a Northern Saw-whet Owl call to monitor for owl presence at various areas above 3,500 feet in elevation. Owls were found at multiple areas in Northeast Tennessee, including Roan Mountain, Unaka Mountain, Rocky Fork, and the Pond Mountain Area. Statistical analysis revealed that these owls were not limited by habitat, showing equal presence in Hardwood habitats along with Spruce and/or Fir habitats. …

Design Of The High-Speed Stereo Radiography System, John C. Ivester Iv

Electronic Theses and Dissertations

Orthopaedic pathologies often involve disruption of the mechanical environment of a joint at/below the mm scale. The ability to measure biomechanical kinematics at the sub-mm scale is essential for obtaining valuable insight into pathologies, but small motions of the joints are difficult to quantify. Estimates of skeletal kinematics are commonly made from optical motion capture systems and markers placed on the skin. The error caused by external marker movement is largely avoided with x-ray motion capture. Dynamic radiography uses a series of x-ray images recorded at high-speed and captures in-vivo joint motion. Uncovering the mechanical foundation of orthopaedic pathologies requires …

Development Of Novel Methods For Analysis Of Autonomic Balance And Gamma Coherence In Autism., Marie Katherine Hensley

Electronic Theses and Dissertations

INTRODUCTION: Autism spectrum disorder (ASD) is a developmental disorder that is characterized by difficulty in social interactions, limited range of interests, and repetitive behaviors. ASD has been shown to negatively affect both ambient vision and the autonomic nervous system (ANS). The objectives of this thesis were to determine the efficacy of two novel therapies, visuo-motor training with ambient prism lenses and repetitive transcranial magnetic stimulation (rTMS), on autonomic balance and EEG gamma coherence. Heart rate variability (HRV) and skin conductance level (SCL) were used as indicators of autonomic function. HRV is an excellent indicator of autonomic balance because it has …

The Impact Of Internet Gis On Access To Water Quality Information, Joseph H. Hoover

Electronic Theses and Dissertations

Empowering citizens to comprehend complex environmental issues affecting their daily lives is essential to sustaining a healthy and informed public. The work of many environmental nongovernmental organizations (ENGOs) and institutions of higher education (IHEs) center around helping their stakeholders become informed of, and in turn, better understand complex environmental problems. However, providing individual stakeholders with knowledge about environmental issues that is easily accessible and understandable represents a recurring challenge in today's society. As a result, a gap continues to exist between that which is known about environmental problems and the public's awareness and understanding of those issues. Arsenic contamination of …

"Punk Has Always Been My School": The Educative Experience Of Punk Learners, Rebekah A. Cordova

Electronic Theses and Dissertations

Punk music, ideology, and community have been a piece of United States culture since the early-1970s. Although varied scholarship on Punk exists in a variety of disciplines, the educative aspect of Punk engagement, specifically the Do-It-Yourself (DIY) ethos, has yet to be fully explored by the Education discipline.

This study attempts to illucidate the experiences of adults who describe their engagement with Punk as educative. To better know this experience, is to also better understand the ways in which Punk engagement impacts learner self-concept and learning development. Through the phenomenological in-depth interviewing of six adult participants located in Los Angeles, …

Dust And Pleistocene Ice Ages: Eolian Sediments And Climate Change At Ziegler Reservoir, Snowmass Village, Colorado, Jeffrey S. Honke

Electronic Theses and Dissertations

The discovery of the Ziegler Reservoir fossil site near Snowmass Village, Colorado presents an opportunity to examine subalpine ecosystem response, during a relatively unknown period, in which climate conditions were similar to present. Finegrained sediments at Ziegler Reservoir represent continuous deposition between ~140–55 ka (thousand years before present), spanning the close of the Bull Lake glacial period [marine isotope stage (MIS) 6], the Sangamon interglacial (MIS 5) and the early stages of the Pinedale (early Wisconsin) glacial period (MIS 4). Ziegler Reservoir is positioned on top of a ridge, at an elevation of 2705 m, and has a small watershed …

Stress Corrosion Cracking In Polymer Matrix Glass Fiber Composites, Jonathan Kosak

Electronic Theses and Dissertations

With the use of Polymer Matrix Glass Fiber Composites ever expanding, understanding conditions that lead to failure before expected service life is of increasing importance. Stress Corrosion Cracking (SCC) has proven to be one such example of conditions found in use in high voltage transmission line applications that leads to brittle fracture of polymer matrix composites.

SCC has been proven to be the result of acid buildup on the lines due to corona discharges and water buildup. This acid leaches minerals from the fibers, leading to fracture at low loads and service life. In order to combat this problem, efforts …

Nitroxide Radicals For Low Frequency Electron Paramagnetic Resonance Imaging (Epri), Joshua R. Biller

Electronic Theses and Dissertations

Optimization of nitroxides as probes for EPR imaging requires detailed understanding of spectral properties such as spin lattice relaxation times, spin packet linewidths, and nuclear hyperfine splitting. Initial measurements of relaxation times for six low molecular weight nitroxides at X-band stimulated further measurement at frequencies between 250 MHz and 34 GHz. The impact of tumbling was studied with perdeuterated 2,2,6,6-tetramethyl-4-piperidinyl-1-oxyl (PDT) in five solvents with viscosities resulting in tumbling correlation times, τ_R, between 4 and 50 ps. A set of three ¹⁴N/¹⁵N pairs of nitroxides in water was selected such that τ_R varied between …

Governing Confidence: Rhetoric, Affect, And Post-Crisis Financial Education, Samuel M. Jay

Electronic Theses and Dissertations

The 2007-08 financial crisis has been characterized as a “crisis of confidence” (Akerlof & Shiller, 2009), a span of time during which the non-discursive energy needed to compel Americans towards profit-producing decisions evaporated. Amidst this decline, the US lost its competitive edge in the global marketplace. Initial responses to the crisis by national leaders failed, triggering a revision to reasoning that resulted in a new argument taken up by central government: the lack of financial knowledge experienced by the majority of US citizens led to a population of ignorant decision makers lacking the confidence needed to take the risks necessary …

How Does Your Garden Grow: How Planting Seeds Of Hope Inspire A Community Of Gifted African-American Learners To Flourish In An Early Childhood Setting, Danielle Elaine Macneal-Harris

Electronic Theses and Dissertations

"Knowledge is like a garden: if it is not cultivated, it cannot be harvested"

-African Proverb

Each day, African-American children are rarely given the opportunity to reach their full potential and flourish in American school systems. There continues to be a disparity in the number of African-Americans in the gifted population. When identified early, and with appropriate educational opportunities, young, culturally diverse gifted learners will be more likely to have long-term educational success. By utilizing an educational criticism methodology, this study discusses the importance of gifted education for African-American, early childhood students, by answering the question, how does The Hope …