Digital Commons Network^™

Regulation Of C-Reactive Protein Gene Expression And Function, Avinash N. Thirumalai

Electronic Theses and Dissertations

Human C-reactive protein (CRP) is the prototypic acute phase protein whose serum concentration increases rapidly during inflammation. CRP is also associated with atherosclerosis; it is deposited at lesion sites where it may interact with modified lipoproteins. There are 2 major questions regarding CRP: 1. How is the serum concentration of CRP regulated? 2. What are the functions of CRP in atherosclerosis?

Our first aim was to determine the role of the constitutively expressed transcription factor Oct-1 in regulating CRP gene expression. We found that Oct-1 overexpression inhibited (IL-6+IL-1β)- induced CRP gene expression; maximal inhibition required the binding of Oct-1 to …

Pathogenic Role Of Acrolein In Alcoholic Liver Disease., Wei-Yang (Jeremy) Chen

Electronic Theses and Dissertations

Alcohol is the most socially accepted addictive drug, and it can cause alcoholic liver disease (ALD), which is a major cause of morbidity and mortality in the United States and worldwide. Animal and human studies demonstrate that chronic alcohol consumption causes a pro-oxidant environment in the liver and increases hepatic lipid peroxidation and the accumulation of by-products such as acrolein and 4-hydroxynonenal. Acrolein is the most reactive and toxic aldehyde generated through lipid peroxidation. Also, acrolein is a major component of cigarette smoke, and there is increasing evidence that smoking negatively impacts the incidence, severity, and clinical course of chronic …

Pathogenic Role Of Phosphodiesterase 4 (Pde4) In The Development Of Alcohol Induced Hepatic Steatosis., Diana Veronica Avila

Electronic Theses and Dissertations

Alcohol induced hepatic steatosis is a significant risk factor for progressive liver disease. Steatotic hepatocytes have increased sensitivity to injury produced by inflammatory cytokines, particularly TNF. Cyclic adenosine monophosphate (cAMP) has been shown to play a significant role in the regulation of both TNF production and lipid metabolism. However, the role of altered cAMP homeostasis in alcohol mediated hepatic steatosis and injury has not been studied. cAMP levels are tightly regulated by phosphodiesterase family of enzymes. Our recent work demonstrated that increased expression of hepatic PDE4, which specifically hydrolyzes and decreases cAMP levels, plays a pathogenic role in the development …

Prompting Discussion : Writing Prompts, Habits Of Mind, And The Shape Of The Writing Classroom., William Matthew Wiles

Electronic Theses and Dissertations

Much of the important writing students will be tasked with in their college careers calls upon them to approximate the writing styles academics and professionals use to shape and advance their respective fields. Many disciplinary values are encoded within the such texts. Learning these styles can be difficult for students who lack the experience and ingrained habits that their instructors may take for granted. In most cases, college writers are outsiders peeking in from the outskirts of academic and professional discourse. Also, the “Expert Blind Spot” (Ambrose et. al) can make college instructors oblivious to the nuances of the writing …

Pedagogy : Reconsiderations And Reorientations., John Vance

Electronic Theses and Dissertations

This dissertation is a critical intervention into the question of student agency. An interdisciplinary project that draws upon philosophy and linguistics, it reviews four major tendencies that have animated composition pedagogy over the last several decades— process theory, social-constructivism, procedural rhetoric, and trans-lingual pedagogies— and identifies some of the key tensions that both motivate and problematize these approaches. First, it examines the debate between Peter Elbow and David Bartholomae, and the interplay between teachers’ authority and student agency. Second, it explores the imbrications between representation and materiality in social constructivism. Third, it uses Alain Badiou’s Being and Event to analyze …

Lunasin Reduces The Melanoma Stem Cell Population In Vitro And Inhibits Tumor Proliferation In Vivo., Christopher Paul Shidal

Electronic Theses and Dissertations

Lunasin is a 44 amino acid peptide derived from the soybean seed that has been shown to have cancer chemopreventive and chemotherapeutic properties. In this study, we investigated the potential utility of lunasin as a chemotherapeutic in a melanoma model. Initial studies showed that lunasin has little activity against established melanoma cell lines in vitro using adherent culture methods; however, lunasin’s in vitro activity was significantly higher in non-adherent colony-forming assays in soft agar and oncosphere assays. These results led us to investigate whether lunasin selectively affects cancer initiating cells (CIC) that are known to be present in these melanoma …

The Effect Of Infrastructure Changes On Railway Operations., Martin J.R. Schmidt 1966-

Electronic Theses and Dissertations

This paper makes use of standard simulation programs in combination with the tools of applied statistics to simulate railway operations. The purpose of the use of this tool is to evaluate and compare different possible kinds of railway infrastructure, like different types of signaling procedures, different network configuration or operational procedures. A railway system is a logistic network and because of the demand for improved railway operation, much work has been undertaken lately in this scientific field. However the author postulates the hypothesis based on a literature review that in a lot of these works there is a lack of …

Biomarker Discovery Of Liver Diseases Using Mass Spectrometry-Based Metabolomics., Xue Shi

Electronic Theses and Dissertations

Metabolomics has emerged as one of the latest of the "-omics" disciplines that can detect metabolite biomarkers in biological samples. The diverse characteristics of metabolites make the analytical platform challenging in metabolomics. Two bioanalytical platforms were developed in this study to investigate metabolite abundance changes under different biological conditions. We first developed a bioanalytical platform that coupled linear trap quadruple - Fourier transform ion cyclotron mass spectrometer (LTQ-FTICR MS) with direct infusion chip-based nano-electrospray ionization (DI-nESI) and applied it to study polychlorinated biphenyls (PCB) effects on non-alcoholic fatty liver disease (NAFLD). We also employed this platform in conjunction with in …

Cloning And Expression Of A Tobacco Stearoyl-Acp Desaturase Gene Sbip24 And Its Interaction With Sabp2 In Sa Pathway, Amin J. Ferdous

Electronic Theses and Dissertations

Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infected transgenic C3 (control tobacco plant containing empty silencing vector) and 1-2 (SABP2-silenced) …

Impulsivity, Venturesomeness, And Pride: Potential Moderators Of The Relationship Between Childhood Trauma, Substance Use, And Physical Aggression, Joshua P. Hatfield

Electronic Theses and Dissertations

Impulsivity, venturesomeness, and pride variables were examined as potential moderators of the associations between childhood trauma and physical aggression, alcohol use and physical aggression, and drug use and physical aggression. Participants (n = 457) were college students recruited from a university in the Southeast. It was hypothesized that childhood trauma, alcohol use, and drug use would be associated with increased scores of physical aggression. In addition, it was hypothesized that impulsivity, venturesomeness, authentic pride, and hubristic pride would moderate these relationships. Linear, multivariate hierarchical regression analyses were used to examine these variables as potential moderators. Hypotheses concerning hubristic pride as …

Brain Based Learning: K-12 Teachers’ Preferred Methods Of Science Instruction, Donna L. Mansy

Electronic Theses and Dissertations

The purpose of this quantitative study was to investigate Brain Based Learning (BBL) techniques in teaching science. Participants included 216 K-12, full-time, regular education teachers from 8 Northeast Tennessee school systems who taught at least 1 science class. Specifically this research was guided by 7 research questions on teachers’ perceptions and practices in teaching science.

Data were collected by a survey that consisted of 82 statements where teachers rated their level of agreement and was distributed online via Survey Monkey. The first portion of my survey included demographic identifiers, teachers’ knowledge of the term BBL, and inquiries regarding science background …

Cellular-Based Brain Pathology In The Anterior Cingulate Cortex Of Males With Autism Spectrum Disorder, Jessica D. Crawford

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) now affects 1 in 68 children in the United States. Disorders within this spectrum share hallmark deficits in verbal and nonverbal communication, repetitive behavior, and social interaction. The cause of ASD is still unknown. Even though hundreds of genetic abnormalities have been identified in ASD, these markers account for less than 1% of all ASD cases. Researchers continue to search for pathological markers common to all or most cases of ASD. The research presented in this dissertation used a novel combination of state-of-the-art methods to investigate brain pathology in ASD. Postmortem anterior cingulate cortex (ACC) from …

Hif-1Α In The Heart: Provision Of Ischemic Cardioprotection And Remodeling Of Nucleotide Metabolism, Joe Wu

Electronic Theses and Dissertations

In our studies we found that stabilized expression of HIF-1α in heart led to better recovery of function and less tissue death after 30 minutes of global ischemia, via mechanisms that preserve the mitochondrial polarization. Our group previously showed that HIF-1α conferred ischemic tolerance by allowing cardiomyocytes to use fumarate as an alternative terminal electron acceptor to sustain anaerobic mitochondrial polarization. The source of fumarate was identified as the purine nucleotide cycle (PNC). Here we discovered that HIF-1α upregulates AMP deaminase 2 (AMPD2), the entry point to the PNC. The combination of glycolysis and the PNC may protect the heart's …

A Comprehensive Study Of The Effects Of Neurotoxins On Noradrenergic Phenotypes, Neuronal Responses And Potential Intervention By Antidepressants In Noradrenergic Cells, Yan Wang

Electronic Theses and Dissertations

It has been reported that locus coeruleus (LC) degeneration precedes the degeneration of other neurons in the brain in some neurodegenerative diseases like Alzheimer’s disease (AD) and Parkinson’s disease (PD). However, the precise mechanisms of neurodegeneration remain to be elucidated. N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) has been widely used as a noradrenergic neurotoxin in the development of AD and PD animal models for specific LC degeneration. However, the precise mechanism of action of DSP4 remains unclear. An increased systemic DNA damage caused by neurotoxin or oxidative stress has been found to be related to the pathogenic development of neurodegeneration. The process of neurodegeneration …

Identification, Cloning, And Expression Of Tobacco Responsive To Dehydration Like Protein (Rd22), Sbip-355 And Its Role In Sabp2 Mediated Sa Pathway In Plant Defense, Hanadi Abdulaali Almazroue

Electronic Theses and Dissertations

Abscisic acid (ABA) induces RD22, responsive to dehydration stress gene. Salicylic acid (SA) has been the focus of research due to its role against pathogens and abiotic stress. Interaction between ABA and SA signaling pathways is still poorly understood. SA-Binding Protein 2 (SABP2) converts methyl salicylate to SA. An attempt was made to identify proteins that interact with SABP2 using a yeast 2-hybrid screening. Several interactors were identified. One of them, SA-Binding Protein 2 Interacting Protein-355 (SBIP-355), showed high homology to RD22. Bioinformatic approaches showed that SBIP-355 contains a BURP domain. Phylogenetic analysis reveals that SBIP-355 clustered into the …

The Wolf Attacks: A History Of The Russo-Chechen Conflict, Christina E. Baxter

Electronic Theses and Dissertations

In the 1990s and early 2000s, the Chechens fought against the Russians for independence. The focus in the literature available has been on the wars and the atrocities caused by the wars. The literature then hypothesizes that the insurgency of today is just a continuation of the past. They do not focus on a major event in Chechen history: the Soviet liquidation of the Chechen-Ingush Autonomous Soviet Socialist Republic in 1944. It is this author’s assertion that the liquidation of the Chechen-Ingush ASSR forever changed the mindset of the people because it fractured a society that was once unified. This …

Development And Testing Of A Rapid Multiplex Assay For The Identification Of Biological Stains, Kevin M. Legg

Electronic Theses and Dissertations

While DNA profiling makes it possible to individualize biological stains, the identification of the stain itself can present forensic serologists with a significant challenge. Current antibody- and enzyme activity-based assays used by forensic practitioners for biological stain identification yield only presumptive results. Positive results with non-target body fluids or cross-reactivity with non-human sources has also been well documented. Some tests can consume unacceptable quantities of precious evidence while for some body fluids (vaginal fluid and menstrual blood) there are simply no available tests at all.

This research presented here aims to develop and rigorously test a fast, accurate, and sensitive …

Analyzing The Interactions Between Xenopus Tropicalis Mc2r, Mc5r, And The Mraps: Modeling The Regulation Of Frog Interrenal Cells, Yesenia Garcia

Electronic Theses and Dissertations

The role of Melanocortin 2 Receptor (MC2R) in adrenal/interrenal glucocorticoid secretion has been well documented in many organisms. Studies in mammals have shown that in the adrenal gland two melanocortin receptors and two melanocortin receptor accessory proteins are expressed: MC2R, MC5R, MRAP, and MRAP2. The MRAPs have an opposite effect on the cell surface expression of MC2R and MC5R. In mammals, MRAP aids MC2R but inhibits MC5R cell surface expression. This thesis aims to explore the functional relationship between MC2R, MC5R, MRAP, and MRAP2 in Xenopus tropicalis to determine if the MRAPs have a similar effect on amphibian MC2R and …

Longitudinal Perspective Of Participation In A Double Blind Placebo Surgery Trial, Jessica Kuhne

Electronic Theses and Dissertations

Parkinson’s disease (PD) is a chronic and progressive neurological disorder that causes both motor symptoms and non-motor symptoms in individuals. Overall, PD impacts the physical, emotional and social functioning in the lives of those impacted by the disorder. In 2001, Freed et al. investigated the effects of fetal tissue transplantation in participants with PD by conducting a double-blind sham-controlled surgery trial. The quality of life (QoL) study was conducted concurrently by McRae et al. (2004) in order to determine whether QoL improved in participants in the transplant group compared to the sham group after the one-year period of the double-blind. …

Obstructive Sleep Apnea Syndrome And Neuropsychological Dysfunction: Understanding The Impact Of Obesity, Chelsea A. Hilsendager

Electronic Theses and Dissertations

Obesity is the largest risk factor for the development of obstructive sleep apnea syndrome (OSAS), which is a common sleep-related breathing disorder that is associated with cognitive dysfunction. A growing body of literature suggests that obesity is related to negative neuropsychological outcomes independent of other health problems known to impact cognitive functioning (e.g., type II diabetes, cardiovascular disease). The purpose of this study was to investigate the impact of obesity on the cognitive functioning of individuals with OSAS. Specifically, this study aimed to examine whether or not differences existed between obese and non-obese persons with OSAS on a battery of …

A Study Of Fetal Tissue Implantation For The Treatment Of Parkinson's Disease: Can Self-Efficacy And Social Support Predict Physical Functioning And Perceived Treatment?, Bethany L. Fiebelkorn

Electronic Theses and Dissertations

Few studies have comprehensively explored the benefit of fetal tissue implantation in Parkinson's disease (PD) patients. This type of investigation is necessary to better understand how and why some participants in a double-blind investigation of fetal tissue implantation for PD improved following the surgical procedure while others did not (Freed et al., 2001). Data for the present study were collected during a quality of life (QoL) study conducted by McRae et al. (2004) that sampled PD patients who participated in Freed et al.'s (2001) double-blind investigation of fetal tissue implantation. Several aspects of QoL were examined in McRae et al.'s …

Studying Eye Gaze Of Children With Autism Spectrum Disorders In Interaction With A Social Robot, Huanghao Feng

Electronic Theses and Dissertations

Children with Autism Spectrum Disorders (ASDs) experience deficits in verbal and nonverbal communication skills including motor control, emotional facial expressions, and eye gaze attention. In this thesis, we focus on studying the feasibility and effectiveness of using a social robot, called NAO, at modeling and improving the social responses and behaviors of children with autism. In our investigation, we designed and developed two protocols to fulfill this mission. Since eye contact and gaze responses are important non-verbal cues in human's social communication and as the majority of individuals with ASD have difficulties regulating their gaze responses, in this thesis we …

A Comparison Of Two Method For Dg Optimizing In Power System, Yu Li

Electronic Theses and Dissertations

In this paper, we mainly focus on using new method to solve the DG problem in order to reducing the loss. We start from comparing two new method. We list out the mathematics theory of the two methods. And then, we using a 33 bus modeling to show which method has a better function on reducing the loss for system. After that, we take the better method to reducing the loss for two cases (14 bus , 34 bus modeling). At last, we conclude the result of the research and give some comment on the future works.

Behind The Doors Of White Supremacy, Catlyn Kenna Keenan

Electronic Theses and Dissertations

What is the relationship between religious belief and acts of violence and terrorism? The American white supremacy movements of the Ku Klux Klan and Christian Identity are deeply rooted in Protestant Christianity and are also responsible for some of the gravest acts of violence on American soil. The intersection between religious belief and terrorist action illuminates the interplay between religion and acts of violence.

This study firstly provides a history of the ideology of Christian Identity with specific attention to the ways in which committing violence is rationalized and understood as redemptive. Secondly, this study identifies six characteristics of a …

Thermal And Structural Modeling Of Electrochromics On Glass, Mahesh B. Manandhar

Electronic Theses and Dissertations

Advanced glazing is a type of window glazing that interacts with the surrounding environment to change its thermal and optical properties to create a more comfortable and energy efficient indoor environment. Electrochromics are one type of such advanced glazing materials that change their properties in response to an externally applied voltage controllable by the user or by automation.

The effectiveness of electrochromics depends upon how well it can keep off extreme temperature conditions from outdoors all year round as well as how structurally stable it can remain at such conditions. This research investigates the use of COMSOL, a finite element …

The Problem Of The Twenty-First Century In The Cherokee Nation Is The Problem Of The Color-Line : How The Cherokee Freedmen Have Articulated A Sense Of Cultural Identity And Citizenship Claims Over Time., Shannon Spears

Electronic Theses and Dissertations

This research investigates the Cherokee Freedmen, who are people of African-American descent and peoples of mixed African-American and Native American descent, who were once enslaved by Cherokees in the Cherokee Nation, and who have articulated their identity and tribal citizenship status as Cherokee Natives in the Cherokee Nation. Upon emancipation, the Cherokee Nation adopted the Cherokee Freedmen as equal citizens in the Cherokee Nation under article nine from the Treaty of 1866, but this changed after citizens were recorded on the Dawes Rolls based upon blood quantum. The theoretical framework of this research is Antonio Gramsci’s theoretical perspective of cultural …

Building A Metabolic Bridge Between Glycolysis And Sphingolipid Biosynthesis : Implications In Cancer., Morgan L. Stathem

Electronic Theses and Dissertations

Cancer therapeutics has seen an emergence and re-emergence of two metabolic fields in recent years, namely bioactive sphingolipids and glycolytic metabolism. Anaerobic glycolysis and its role in cancer has been recognized in cancer biology over 90 years. In recent decades, the role of sphingolipids in cancer cell metabolism has gained recognition, notably ceramide’s essential role in programmed cell death and the role of the glucosylceramide synthase (GCS) in chemotherapeutic resistance. Despite this knowledge, a direct link between these two fields has yet to be concretely drawn. Herein, we show that in a model of highly glycolytic cells, generation of the …

Inducible Tumor Difference Between Rapid And Slow Rat Nat2 Congenic Fischer 344 Rats Administered Methyl-Nitrosourea., Marcus W. Stepp

Electronic Theses and Dissertations

Human arylamine N-acetyltransferase 1 (NAT1) is a well-known phase II metabolic enzyme that has been associated with carcinogenesis. Its role in the biotransformation of aromatic and heterocyclic amine carcinogens has been investigated for many years, but more recent investigations focus on a possible endogenous role of human NAT1 in cancer initiation and progression. We conducted in vivo studies using homozygous Fischer 344 rats, congenic at the rat Nat2 locus for high (rapid) and low (slow) activity. Wistar Kyoto inbred rats were used to breed in the slow activity rat Nat2 locus into the Fischer 344 inbred rat, which contains the …

Effects Of Passive Immobilization On Locomotor Recovery After Spinal Cord Injury In Adult Rats., Kelsey Lee Stipp

Electronic Theses and Dissertations

Background: Spontaneous locomotor recovery in spinal rats has been attributed to animals moving freely in-cage. Environmental enrichment has been shown to increase in-cage movement and functional recovery subsequently. Anxiety has been shown to decrease overnight activity in rats. Methods: Rats were double-housed in medium cages (MC) or single-housed in tiny sized cages (TC). Slotted dividers allowed for partial isolation in TC. Overnight activity was monitored bi-weekly. The open field test and BBB’s were taken weekly. Gait analysis was performed at weeks six and eight. Results: MC showed higher overnight activity and improved gait overtime. No differences were found in BBB …

Penalized Regressions For Variable Selection Model, Single Index Model And An Analysis Of Mass Spectrometry Data., Yubing Wan

Electronic Theses and Dissertations

The focus of this dissertation is to develop statistical methods, under the framework of penalized regressions, to handle three different problems. The first research topic is to address missing data problem for variable selection models including elastic net (ENet) method and sparse partial least squares (SPLS). I proposed a multiple imputation (MI) based weighted ENet (MI-WENet) method based on the stacked MI data and a weighting scheme for each observation. Numerical simulations were implemented to examine the performance of the MIWENet method, and compare it with competing alternatives. I then applied the MI-WENet method to examine the predictors for the …