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Folate Conjugated Hyperbranched Polyester Nanoparticles For Prostate Tumor-Targeted Delivery Of A Cytotoxic Peptide Via Prostate Specific Membrane Antigen, Orielyz Flores-Fernandez
Folate Conjugated Hyperbranched Polyester Nanoparticles For Prostate Tumor-Targeted Delivery Of A Cytotoxic Peptide Via Prostate Specific Membrane Antigen, Orielyz Flores-Fernandez
Electronic Theses and Dissertations
Prostate Cancer is the second most deadly cancer in men, after lung cancer. The need for new and effective therapeutics that can constrain prostate cancer progression are challenged by the lack of suitable delivery strategies that target prostate cancer tissue. To study CT20p as potential chemotherapeutic agent in the treatment of prostate cancer we proposed the use of targetable hyperbranched polyester (HBPE) based nanoparticles as delivery system. Folic acid was conjugated to the nanocarrier to improve the selectivity of the nanoparticle towards specific cell surface targets in prostate cancer cell lines. Specifically we evaluated LNCaP that up-regulated the PSMA receptor. …
The Impact Of Photopollution On Nesting Loggerhead Sea Turtles (Caretta Caretta) Along The Grand Strand, South Carolina, Ryan Bonner
Electronic Theses and Dissertations
Photopollution present along heavily developed coastlines is known to have a significant negative impact on nesting sea turtle populations by damaging nesting habitat quality, but few studies have explored this relationship on broad spatial scales. The purpose of this study was to characterize the onshore light field along a developed stretch of South Carolina coastline and to quantify how photopollution intensity affects regional loggerhead sea turtle (Carretta carretta) nesting density using historical nesting data. Light measurements were collected using a portable low-light photometer at 74 sites spaced at approximately one km intervals across the study area. At each site, the …
Impact Of Development On Freshwater Turtle Populations Of Coastal South Carolina: An Examination Of Land Use And Road Density, Andre Dominguez
Impact Of Development On Freshwater Turtle Populations Of Coastal South Carolina: An Examination Of Land Use And Road Density, Andre Dominguez
Electronic Theses and Dissertations
Previous studies (Haxon 2000; Steen and Gibbs 2004; Marchand and Litvaitis 2004; Aresco 2005) have demonstrated that urbanization and development have disproportionally adverse affects on female turtles. In order to test this paradigm I caught 301 turtles (primarily Trachemys scripta scripta) in ten ponds (5 impacted and 5 unimpacted) across coastal South Carolina. Not only do coastal South Carolina turtles not follow the same male biased pattern of many other studies, there was an overall female bias (154:95). Turtle metrics were between the types of sites existed as well, indicating larger/older population of turtles in unimpacted sites. Water quality parameters …
Tide-Dependent Predation Patterns Of Piscivorous Fishes In Shallow Estuarine Creeks, Matthew Ross Helms
Tide-Dependent Predation Patterns Of Piscivorous Fishes In Shallow Estuarine Creeks, Matthew Ross Helms
Electronic Theses and Dissertations
The distribution and foraging behavior of predatory salt marsh fishes is shaped by the movements of prey, many of whom seek shelter in shallow and tributary creeks. I hypothesized that the distribution of piscivorous fishes in marsh creek channels would differ with proximity to the mouths of intertidal creeks and with tidal stage. Custom-built trotlines baited with live minnows were deployed during four discrete tidal stages at two microhabitats in main creek channels: (1) adjacent to intertidal creek mouths, and (2) along straight banks in North Inlet estuary, South Carolina. Catch-per-unit-effort of predatory fishes was significantly higher (Wilcoxon signed-rank tests, …
The Crypt People From The Cathedral Basilica Of Saints Stanislaus And Vladislaus, Vilnius, Lithuania: Reconstruction Of Life Histories Using Stable Isotope Analysis, Kaylee Schotten
Electronic Theses and Dissertations
Stable isotope analyses of multiple tissue types have been used to reconstruct life histories of individuals from past populations. This thesis presents the life history reconstructions of a unique population recovered from a 16th to 18th century cathedral crypt located in Vilnius, Lithuania. The sample consists of 23 individuals (21 males, one possible female, and one juvenile). Stable carbon, nitrogen, and oxygen isotope analyses were performed on bone and dentin collagen, and on bone and enamel hydroxyapatite, resulting in eight isotope values per individual, providing both childhood and adult dietary and migration information. For bone collagen the average δ13C isotope …
Timp-1 Activates A Unique Cardiac Stem Cell Population, Cd63+Ve/C-Kit+Ve, Thereby Enhancing Cardiac Differentiation, And Protects The Heart From Adverse Cardiac Remodeling Following Myocardial Infarction, Latifa Abdelli
Electronic Theses and Dissertations
We previously demonstrated that embryonic stem (ES) cells over-expressing tissue inhibitor of metalloproteinase-1 (TIMP-1) have increased potential to engraft and differentiate into cardiac myocytes following transplantation into the infarcted heart. However, the ability of TIMP-1 to activate endogenous stem cells and enhance their differentiation into cardiac regenerative cell types is still unknown. We postulate that TIMP-1 may additionally activate a stem cell population that enhances cardiac cell type differentiation in the infarcted myocardium. To prove this hypothesis, we isolated c-kit+ve cells from four weeks old C57BL/6 mice and cultured them in vitro in presence of ES conditioned media (ESCM), ES-TIMP-1-CM …
Ode To Amiel: A Micro-Budget Experimental Essay Film, Phyllis Redman
Ode To Amiel: A Micro-Budget Experimental Essay Film, Phyllis Redman
Electronic Theses and Dissertations
Ode to Amiel is a feature-length experimental essay film by Phyllis Redman, made as part of the requirements for earning a Master of Fine Arts in Film in the Entrepreneurial Digital Cinema track from the University of Central Florida. The film explores one woman's reaction to trauma and depersonalization disorder through the journal entries of 19th Century Swiss philosopher, Henri Frederic Amiel. Passages from Amiel's Journal Intime provide the narrative and voice over for the lead character, a grieving mother who finds herself locked behind an inescapable, invisible and immaterial barrier that separates her from the outside world. Following the …
Wise The Wizard: A Feature Documentary About The Late Harry Wise Of Sanford, Florida, Daisy Blakelock
Wise The Wizard: A Feature Documentary About The Late Harry Wise Of Sanford, Florida, Daisy Blakelock
Electronic Theses and Dissertations
Wise the Wizard is a feature-length documentary by Daisy Sara Blakelock, made as part of the requirements for earning a Master of Fine Arts in Entrepreneurial Digital Cinema from the University of Central Florida. The film focuses on the late Harry Wise, a magician from Sanford, Florida, as remembered by the people who knew him best. Interview subjects include the following: author and TV Producer Charlie Carlson, who wrote a biography about Harry Wise entitled A Wizard's Tux and Tales (Luthers Press, New Smyrna Beach: 2004); Brendan and Anna McWilliams, who accompanied Harry on countless adventures throughout Sanford and the …
Development Of Models For The Study Of The Molecular Mechanisms Of Host Restriction And Adaptation Of Hantaviruses., Ryan Carroll Mcallister 1988-
Development Of Models For The Study Of The Molecular Mechanisms Of Host Restriction And Adaptation Of Hantaviruses., Ryan Carroll Mcallister 1988-
Electronic Theses and Dissertations
Hantaviruses, family Bunyaviridae, are present throughout the globe in a variety of mouse, rat, mole, vole, shrew, or bat species. Hantaviruses persist for the lifetime of the animal reservoir, while causing no signs or symptoms of disease. Only the rodent-borne hantaviruses cause disease in humans. In contrast, a “spillover” infection of a hantavirus into a nonreservoir rodent species results in an asymptomatic acute infection. We and others in the field are interested in understanding the biology of these virus-host interactions and mechanisms that underlie these three very different outcomes. The second chapter of my thesis focused on probing the intrahost …
Prankster Questionaire., Matthew Landan 1972-
Prankster Questionaire., Matthew Landan 1972-
Electronic Theses and Dissertations
The creative thesis that I have written is a memoir. It is not complete in its detail or ambition but instead focuses on themes and topics of self-transformation, reinvention and the unintentional consequences of several nervous breakdowns and experiments with psychedelic substances. This thesis is just a beginning for a longer more drawn out memoir that will explore the themes above but also discuss family, one’s sense of self worth in the world and how patterns between fathers and sons repeat themselves. I hope to eventually publish this work in a commercial setting.
Retinal Synaptic Function In The Absence Of The On Pathway., Kathryn Marie Heath Fransen
Retinal Synaptic Function In The Absence Of The On Pathway., Kathryn Marie Heath Fransen
Electronic Theses and Dissertations
Complete Congenital Stationary Night Blindness (cCSNB) is a rare hereditary retinal disorder characterized by abnormal night vision. cCSNB is caused by postsynaptic defects in On bipolar cells (BCs) and is identified by the presence of an electroretinogram (ERG) with a normal a-wave, corresponding to photoreceptor function, and the absence of a b-wave, corresponding to a failure of On BC function. Through the study of genetic mutations in mouse that result in no b-wave ERG phenotypes, several proteins have been identified that play crucial roles in On BC signal transmission. I focused on four mouse models of cCSNB; Nyxnob (Nyctalopin mutant), …
Unnatural Pedagogy : A Computational Analysis Of Children's Learning To Learn From Other People., Baxter S. Eaves 1984-
Unnatural Pedagogy : A Computational Analysis Of Children's Learning To Learn From Other People., Baxter S. Eaves 1984-
Electronic Theses and Dissertations
Infants rely on others for much of what they learn. People are a ready source of quick information, but people produce data differently than the world. Data from a person are a result of that person's knowledgeability and intentions. People may produce inaccurate or misleading data. On the other hand, if a person is knowledgeable about the world and intends to teach, that person may produce data that are more useful than simply accurate data: data that are pedagogical. This idea that people have special innate methods for efficient information transfer lies at the heart of recent proposals regarding what …
Avatar Captcha : Telling Computers And Humans Apart Via Face Classification And Mouse Dynamics., Darryl Felix D’Souza
Avatar Captcha : Telling Computers And Humans Apart Via Face Classification And Mouse Dynamics., Darryl Felix D’Souza
Electronic Theses and Dissertations
Bots are malicious, automated computer programs that execute malicious scripts and predefined functions on an affected computer. They pose cybersecurity threats and are one of the most sophisticated and common types of cybercrime tools today. They spread viruses, generate spam, steal personal sensitive information, rig online polls and commit other types of online crime and fraud. They sneak into unprotected systems through the Internet by seeking vulnerable entry points. They access the system’s resources like a human user does. Now the question arises how do we counter this? How do we prevent bots and on the other hand allow human …
Modulation Of Host Polyubiquitination By The Ankb F-Box Protein Of Legionella Pneumophila., William M. Bruckert
Modulation Of Host Polyubiquitination By The Ankb F-Box Protein Of Legionella Pneumophila., William M. Bruckert
Electronic Theses and Dissertations
Legionella pneumophila is a facultative intracellular pathogen that infects a wide array of protozoan hosts and human alveolar macrophages. L. pneumophila is dependent on a functional Dot/Icm type IVB secretion system that translocates bacterial effector proteins into the host cell cytosol. L. pneumophila genomes encode more than 250 effector proteins, many of which inhibit host cellular processes to form a favorable niche termed the Legionella-containing vacuole (LCV). The eukaryotic-like Dot/Icm translocated effector AnkB contains two eukaryotic-like ankyrin protein-protein interacting domains, one eukaryotic-like F- box domain and an eukaryotic C-terminal CaaX motif. Immediately following attachment of extracellular bacteria, AnkB is translocated …
Evaluating The Effects Of Aroclor 1260 In Non-Alcoholic Fatty Liver Disease : The Role Of Xenobiotic Receptors., Banrida Wahlang
Evaluating The Effects Of Aroclor 1260 In Non-Alcoholic Fatty Liver Disease : The Role Of Xenobiotic Receptors., Banrida Wahlang
Electronic Theses and Dissertations
Polychlorinated biphenyls (PCBs) are persistent environmental toxicants, present in 100% of US adults and dose-dependently associated with nonalcoholic fatty liver disease (NAFLD) in epidemiologic studies. PCBs are predicted to interact with receptors previously implicated in xenobiotic/energy metabolism and NAFLD. These receptors include the aryl hydrocarbon receptor (AhR), pregnane xenobiotic receptor (PXR), constitutive androstane receptor (CAR), peroxisome proliferator-activated receptors (PPARs), liver-X-receptor and farnesoid-X-receptor. This study evaluated the hepatic effects of the PCB mixture, Aroclor 1260, whose composition mimics human bioaccumulation patterns, in a mouse model of diet-induced obesity. Male C57Bl/6J mice were fed a control or 42% high fat diet (HFD) …
The Role Of Stem Cells In Adipose Tissue Remodeling., Candice Reshay Holden 1988-
The Role Of Stem Cells In Adipose Tissue Remodeling., Candice Reshay Holden 1988-
Electronic Theses and Dissertations
The work presented in this dissertation outlines the role of stem cells in the remodeling of adipose tissue under conditions of nutrient excess. Obesity-induced variations in adipose tissue stem cell distribution were uncovered by analysis of the stromal vascular fraction isolated from mice fed a high fat diet for several weeks. Bone marrow cell transplantation was used to determine the derivation of progenitor cells found in adipose tissue; and supplementation of depleted progenitor cell populations achieved via direct cell transplantation, helped to determine the contribution of these progenitor cells to the remodeling process. The dissertation is presented in five chapters …
Temporal Contextual Descriptors And Applications To Emotion Analysis., Haythem Balti
Temporal Contextual Descriptors And Applications To Emotion Analysis., Haythem Balti
Electronic Theses and Dissertations
The current trends in technology suggest that the next generation of services and devices allows smarter customization and automatic context recognition. Computers learn the behavior of the users and can offer them customized services depending on the context, location, and preferences. One of the most important challenges in human-machine interaction is the proper understanding of human emotions by machines and automated systems. In the recent years, the progress made in machine learning and pattern recognition led to the development of algorithms that are able to learn the detection and identification of human emotions from experience. These algorithms use different modalities …
The Effect Of Spinal Cord Injury On Vagal Afferents., April N. Herrity
The Effect Of Spinal Cord Injury On Vagal Afferents., April N. Herrity
Electronic Theses and Dissertations
Spinal cord injury (SCI) is a significant public health concern that leaves patients with a multitude of life-long disabilities. Major complications of SCI apart from paralysis, include deficits in bladder and bowel function. Lower urinary tract dysfunction continues to remain a top priority issue affecting quality of life for this population. The majority of visceral organs receive a dual sensory innervation from both spinal nerves as well as the vagus nerve. Following SCI, the vagus nerve is a potential pathway through which information from regions below the level of a spinal injury can travel directly to the brainstem, bypassing the …
Signaling Mechanisms In Regenerative Myogenesis., Sajedah M. Hindi 1986-
Signaling Mechanisms In Regenerative Myogenesis., Sajedah M. Hindi 1986-
Electronic Theses and Dissertations
Skeletal muscle constitutes a highly plastic and malleable tissue that rapidly and profoundly adapts to various environmental and physiological stimuli. This response takes place by means of regulated signaling transduction which elicits changes in gene expression, biochemical, and metabolic properties. Adult muscle is composed by alignment of multinucleated, post mitotic muscle fibers formed during embryonic and neonatal development. Residing in the niche of mature muscle fibers is a small population of undifferentiated muscle progenitors termed satellite cells. Satellite cells are muscle stem cells that carry out regenerative response in adult myofibers. Coordinated signaling emitting from terminally differentiated muscle fibers and …
Post-Transcriptional Regulation Of The Type Iia Sodium-Phosphate Cotransporter By Parathyroid Hormone., Rebecca Dale Murray
Post-Transcriptional Regulation Of The Type Iia Sodium-Phosphate Cotransporter By Parathyroid Hormone., Rebecca Dale Murray
Electronic Theses and Dissertations
The type IIa sodium-phosphate cotransporter (Npt2a) is expressed in the apical membrane of the renal proximal tubule and is responsible for the reabsorption of the majority of the filtered load of phosphate. Parathyroid hormone (PTH) is secreted by the parathyroid gland in response to a decrease in serum calcium or an increase in serum phosphorus, and acutely induces phosphaturia through the rapid stimulation of endocytosis of Npt2a and its subsequent lysosomal degradation. Chronic PTH stimulation leads to sustained phosphaturia, but the mechanisms for the chronic regulation of Npt2a by PTH remain unclear. We hypothesize that PTH decreases Npt2a mRNA levels …
Extracellular Matrix Proteins And The Liver-Lung Axis In Disease., Veronica L. Massey
Extracellular Matrix Proteins And The Liver-Lung Axis In Disease., Veronica L. Massey
Electronic Theses and Dissertations
No abstract provided.
A Mechanism For Trif Adaptor-Biased Signaling By Toll-Like Receptor 4., Joseph Peterson Kolb
A Mechanism For Trif Adaptor-Biased Signaling By Toll-Like Receptor 4., Joseph Peterson Kolb
Electronic Theses and Dissertations
Host cells respond to bacterial lipid A through Toll-like receptor 4 (TLR4). Activation of TLR4 by lipid A triggers a response that involves two main adaptor proteins, MyD88 and TRIF. MyD88-dependent gene expression is associated with proinflammatory protein production, while TRIF-dependent gene expression is essential for optimal activation of adaptive immunity by antigen-presenting cells. Detoxified, monophosphoryl lipid A agonists (MPLA or synthetic MLA) were previously suggested to elicit TRIF-biased TLR4 signaling; that is, induction of weaker MyD88-associated gene expression but relatively intact TRIF-dependent gene expression when compared to fully active diphosphoryl lipid A (lipid A). In this work, we explored …
A Magical Country : Stories From Appalachia., James Eric Leary 1977-
A Magical Country : Stories From Appalachia., James Eric Leary 1977-
Electronic Theses and Dissertations
Fairy tales and other forms of fantastic literature have fascinated children and adults for generations. The scholarship on these types of works indicate that many find their folkloric origins in oral storytelling, and those roots may be quite ancient. However, some of the earliest examples of recorded literature capture stories of magic and fantasy. The interplay between the oral and literary form remains a significant area of study and development for folk literature, and new artistic productions, termed variants in the scholarship, continue to appear frequently in contemporary American culture. The criticism and creative work presented here add to the …
Earthworm, Microbial Biomass, And Leaf Litter Decay Responses After Invasive Honeysuckle Shrub Removal From Urban Woodlands., Robert Preston Pipal
Earthworm, Microbial Biomass, And Leaf Litter Decay Responses After Invasive Honeysuckle Shrub Removal From Urban Woodlands., Robert Preston Pipal
Electronic Theses and Dissertations
Invasive species are one of the leading causes of biodiversity loss and their presence can significantly impact the structure and function of native ecosystems. In this dissertation, the impacts of the invasive honeysuckle shrub, Lonicera maackii, on exotic earthworm populations, leaf litter decay responses, and inorganic and organic nutrient pools in an urban woodland park are assessed. Chapter 1 provides a brief review of honeysuckle's effects on forest ecosystems. Chapter 2 describes honeysuckle’s effects on exotic earthworm populations and the seasonal importance of macroinvertebrates to leaf litter decomposition in urban woodlands. This study revealed that L. maackii promoted higher exotic …
Effects Of Step Training On The Kidneys Following Spinal Cord Injury In Rats., Pradeepa Poudyal
Effects Of Step Training On The Kidneys Following Spinal Cord Injury In Rats., Pradeepa Poudyal
Electronic Theses and Dissertations
Detrusor-sphincter dyssynergia after chronic spinal cord injury (SCI) leads to over-distention, high bladder pressures and vesico-ureteric reflux into the kidney, resulting in repeated infections and pyelonephritis. Given the importance/limited knowledge of SCI effects on the upper urinary tract, this study assessed expression of two proteins in the kidneys of contused (T9) male rats relative to controls that are indicative of tissue stress/damage (TGFβ, CD11b). The impact of two therapeutic rehabilitation strategies, 60 minutes of step training and general exercise, was then assessed on these protein levels relative to non-trained SCI controls, plus the effectiveness of a shorter training duration (30 …
Troglitazone Suppresses Glutamine Metabolism And Tumor Cell Growth Through A Ppar-Independent Mechanism., Miriam R. Reynolds 1965-
Troglitazone Suppresses Glutamine Metabolism And Tumor Cell Growth Through A Ppar-Independent Mechanism., Miriam R. Reynolds 1965-
Electronic Theses and Dissertations
In tumor cells, glutamine metabolism provides anaplerotic carbon for the TCA cycle, fatty acid synthesis, and precursors for the production of nucleotides and glutathione. This metabolic alteration is primarily driven by disruptions in oncogenic or tumor suppressor function and results in glutamine dependency for tumor cell survival. Troglitazone, a PPARγ agonist, has been reported to alter glutamine metabolism. This thesis project addresses whether troglitazone treatment could disrupt glutamine metabolism in glutamine-dependent tumor cells. Results obtained with troglitazone treatment include: dose-dependent inhibition of cell proliferation, glutamine uptake, glutaminolytic protein expression, steady-state ATP levels, and glutamine carbon flux into the TCA cycle. …
The Role Of Porphyromonas Gingivalis In The Regulation Of Epithelial-Mesenchymal Transition Markers In Migration., Akintunde Ojo 1981-
The Role Of Porphyromonas Gingivalis In The Regulation Of Epithelial-Mesenchymal Transition Markers In Migration., Akintunde Ojo 1981-
Electronic Theses and Dissertations
A relationship between periodontal disease and oral squamous cell carcinoma (OSCC) has been demonstrated in recent studies. Porphyromonas gingivalis’ antigen have been found in oral cancer cells. The embryonic program “epithelial-mesenchymal transition” (EMT) is thought to trigger cancer invasion by allowing tumor cell propagation. The objective of this study was to examine the role of P. gingivalis in the expression of ZEB1, vimentin, and repression of miRNA-200 family as EMT prognostic markers in cancer cell metastasis. Human telomerase immortalized gingival keratinocytes (TIGK) cells were challenged with P. gingivalis strain ATCC 33277 in vitro for 24h at MOI 50 and 100. …
Utilizing Visual Attention And Inclination To Facilitate Brain-Computer Interface Design In An Amyotrophic Lateral Sclerosis Sample, David B. Ryan
Utilizing Visual Attention And Inclination To Facilitate Brain-Computer Interface Design In An Amyotrophic Lateral Sclerosis Sample, David B. Ryan
Electronic Theses and Dissertations
Individuals who suffer from amyotrophic lateral sclerosis (ALS) have a loss of motor control and possibly the loss of speech. A brain-computer interface (BCI) provides a means for communication through nonmuscular control. Visual BCIs have shown the highest potential when compared to other modalities; nonetheless, visual attention concepts are largely ignored during the development of BCI paradigms. Additionally, individual performance differences and personal preference are not considered in paradigm development. The traditional method to discover the best paradigm for the individual user is trial and error. Visual attention research and personal preference provide the building blocks and guidelines to develop …
Characterization Of A Putative Phospholipase D ´ Like Gene As A Lipid Signaling Modulator And Its Role In Salicylic Acid Mediated Defense Pathway In Nicotiana Tabacum, Phillip T. Dean
Electronic Theses and Dissertations
Plants are in a perpetual evolutionary arms race with a wide range of pathogens. Their sessile nature has led plants to evolve defense mechanisms that can quickly recognize a unique stressor and deploy a resistance tailored for a specific attack. The salicylic acid (SA) mediated defense pathway has been shown to be one of the major defense tactics plants can initiate to defend themselves against microbial pathogens. Following a pathogen attack high levels of methyl salicylate (MeSA) are produced that can be converted to SA by the enzyme salicylic acid binding protein 2 (SABP2). A yeast two-hybrid screening was performed …
Eating In Opposition: Strategies Of Resistance Through Food In The Lives Of Rural Andean And Appalachian Mountain Women, Veronica A. Limeberry
Eating In Opposition: Strategies Of Resistance Through Food In The Lives Of Rural Andean And Appalachian Mountain Women, Veronica A. Limeberry
Electronic Theses and Dissertations
This thesis examines ways in which rural mountain women of Andean Peru and southern Appalachia use their lived histories and food knowledge in ways that counter Cartesian epistemologies regarding national and international food systems. Using women’s fiction and cookbooks, this thesis examines how voice and narrative reclaim women’s spaces within food landscapes. Further, this thesis examines women’s non-profits and grassroots organizations to illustrate the ways in which rural mountain women expand upon their lived histories in ways that contribute to tangible solutions to poverty and hunger in rural mountainous communities. The primary objective of this thesis is to recover rural …