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The Development Of Silver Nanoparticles As Antiviral Agents, John Christopher Trefry

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Silver nanoparticles (AgNPs) have received tremendous attention for their antimicrobial properties; however, many gaps in knowledge exist. To address these issues, three research objectives were examined. The first objective hypothesized AgNPs can be size selected and concentrated via tangential flow ultrafiltration. The second objective hypothesized a high-throughput method could be developed to screen nanoparticle antiviral-activity and cytotoxicity simultaneously. The third objective hypothesized AgNPs inhibit viruses by preventing viral entry.

For objective one, a tangential flow ultrafiltration scheme was tested on AgNPs synthesized via the Creighton Colloid method. AgNPs were analyzed via transmission electron microscopy. In objective two, an HIV-1 vector …

Amino Acid Substitutions Created In Reverse Transcriptase And Their Influence On Hiv-1 Mutation Frequencies, Amani Saud Alhejely

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As research has shown, a high mutation rate occurs during the reverse transcription RT process because HIV reverse transcriptase fails to correct erroneously incorporated nucleotides during the reverse transcription process. Based on various research articles, a series of amino acid substitutions created in RT were collected to find out their influence on virus mutation frequencies. Two tables were used; one for amino acid substitutions created in RT that increased the HIV-1 mutation rate and another for amino acid substitutions created in RT that decreased the HIV-1 mutation rate. The tables show which amino acid substitutions created in RT decreased or …

Developing A Quantitative Pcr Assay For Detecting Viral Vector Shedding From Animals, Swathee Chinnasamy

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Replication deficient viruses have been used widely for replacing, repairing, and deleting target genes. These recombinant viruses are tested on research animals or patients in clinical trials. Although viral vectors distribute in the body, they are also disseminated into the environment through secretion and excretion processes. By studying the extent of shedding, a proper risk assessment can be performed and appropriate biocontainment can be achieved. Adenoviral and lentiviral vectors were produced from commercially available kits. The transgene present in both vector systems was the lacZ reporter gene encoding for β-galactosidase. Primers and probes were designed for the encapsidation region of …

Flight Physician - December, 2010, Civil Aviation Medical Association

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A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

The Guardian, November 10, 2010, Wright State University Student Body

The Guardian Student Newspaper

Sixteen page issue of The Guardian, the official student-run newspaper for Wright State University. The Guardian has been published regularly since March of 1965.

The Guardian, November 3, 2010, Wright State University Student Body

The Guardian Student Newspaper

Twelve page issue of The Guardian, the official student-run newspaper for Wright State University. The Guardian has been published regularly since March of 1965.

The Guardian, October 27, 2010, Wright State University Student Body

The Guardian Student Newspaper

Twelve page issue of The Guardian, the official student-run newspaper for Wright State University. The Guardian has been published regularly since March of 1965.

Wsu Research News, Fall 2010, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A twenty-four page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Acaricidal Activity Of Eugenol Based Compounds Against Scabies Mites, Cielo Pasay, Kate Mounsey, Graeme Stevenson, Rohan Davis, Larry G. Arlian, Marjorie Morgan, Diann Vyszenski-Moher, Kathy Andrews, James Mccarthy

Biological Sciences Faculty Publications

Background

Human scabies is a debilitating skin disease caused by the “itch mite” Sarcoptes scabiei. Ordinary scabies is commonly treated with topical creams such as permethrin, while crusted scabies is treated with topical creams in combination with oral ivermectin. Recent reports of acaricide tolerance in scabies endemic communities in Northern Australia have prompted efforts to better understand resistance mechanisms and to identify potential new acaricides. In this study, we screened three essential oils and four pure compounds based on eugenol for acaricidal properties.

Methodology/Principal Findings

Flight Physician - July, 2010, Civil Aviation Medical Association

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A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Assessment Of Support Service Needs Of Primary Caregivers Of Hiv And Aids Infected Children: A Review Of Literature And Suggestions For Practice, Barry Eneh

Master of Public Health Program Student Publications

Summary: With the advent of highly active antiretroviral treatment, routine screening and treatment options for pregnant women for perinatally acquired HIV and AIDS, infection is on dramatic decline in the U.S. In spite of the declines and the medical advances, primary and informal caregivers of infected children continue to face innumerable challenges with regard to the availability, access and utilization of non-medical supportive service care needs. HIV/AIDS continues to create unique challenges for infected children and the non-infected family caregivers at multiple levels; impacting their relationships, resources and resilience.

Objective: This literature review identifies and assesses the unique and critical …

Barriers To Accessing Primary Dental Care For The Uninsured/ Underinsured Population In The City Of Cincinnati, Kiana R. Trabue

Master of Public Health Program Student Publications

Despite federal, state and local efforts to provide access to dental services to vulnerable populations such as the uninsured and underinsured, many people still do not receive adequate dental care. This study seeks to identify barriers to primary dental care in the City of Cincinnati. The Cincinnati Health Department (CHD) operates five safety net health centers which provide dental care for the citizens of the Cincinnati service area. Surveys conducted in these centers found that cost was the main reason individuals do not see the dentist regularly. Even in those with dental insurance cost was a major barrier, although most …

The Relationship Of Maternal Age, Trimester Of Entry Into Prenatal Care, Maternal Postpartum Depression, And Race With Birth Weight Of Infants Born In Cincinnati, Ohio, Kelli Kohake

Master of Public Health Program Student Publications

Healthcare, resources, and technology in the United States (U.S.) has improved, yet the U.S. ranks 29th in the world for the highest infant mortality rates – behind other less developed countries. Within the U.S., Ohio (and Hamilton County in particular) has high rates of infant mortality. Low birth weight and preterm births are a major cause of infant mortality. Data analysis was done consisting of descriptive statistics and Analysis of Variance of the prenatal patients served at the Cincinnati Health Department health centers who delivered in 2009, with dependent variables of birth weight and postpartum depression. Independent variables included maternal …

Unmet Medical Need Of Underserved Population In A Safety-Net Organization, Leslie Clem

Master of Public Health Program Student Publications

Background: Safety net providers serve a large proportion of America’s uninsured and low income population. The purpose of this study is to determine if differences in unmet medical needs exist between the provisional and clinic populations, age groups, and sex and race categories. This research study provides information about the population that receives services from Good Neighbor House, a non-profit social service organization in Dayton, Ohio.

Methods: GNH created a survey to assess the unmet medical need of their clients. The instrument contains questions about routine medical care, unmet medical care in the last 12 months, medical bills, and demographic …

Socioeconomic And Environmental Causes For Respiratory Infection Death In Alaska Native Villages, Sarah Mcbeth

Master of Public Health Program Student Publications

Objectives. This ecological study evaluates the association between community characteristics and death rates from pneumonia/influenza, tuberculosis, and infectious diseases in 196 Alaska Native villages.

Methods. Ecological variables studied included percentage of Natives in the population, age distribution, latitude, average household size, socioeconomic status, percentage of homes with complete plumbing and/or kitchens, type of heating fuel used, community alcohol restrictions, level of healthcare access, and residence in specific regions of Alaska. These data come from the 2000 U.S. Census, the Alaska Alcohol Beverage Control Board, and the Alaska Native Tribal Health Consortium. A stepwise analysis of variance using the Proc GLM …

Zoonotic Disease In Ohio: Surveillance, Preparation, And Response, Erik B. Balster

Master of Public Health Program Student Publications

This paper examines the state of public health preparation and response capacity to zoonotic infectious disease in the state of Ohio. A comprehensive literature review of zoonotic disease emergence and re-emergence, the National Veterinary Stockpile (NVS), the One Health Initiative, emergency response, and Ohio policy was performed in conjunction with telephone interviews. This study shows that responsibilities and systems integral to disease preparedness, such as sentinel surveillance and reporting, vary significantly between two key departments: the Ohio Department of Health (ODH) and the Ohio Department of Agriculture (ODA). Consistency and standardization of agency, mirroring the One Health Initiative, must be …

An Assessment Of White-Tailed Deer (Odocoileus Virginianus) Dynamics, Management, And Impacts In Highly Fragmented Landscapes, Bambi Shafer

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Habitat and forage selection by deer can cause local extinction of plant species. In my research, I asked: 1) How do deer population growth rates in urban parks change through time? 2) Are culling rates and deer densities correlated? 3) Are browse rates on Trillium spp. and deer densities correlated? I used FLIR deer census data from Davis Aviation and culling data from Five Rivers MetroParks (Montgomery County, Ohio). I found that deer population growth rates generally declined from 2003 to 2008. The number of deer culled was positively correlated to the deer densities. The browse rates of Trillium spp. …

The Im-9 Cell Line: A Model For Evaluating Tcdd-Induced Modulation Of The Polymorphic Human Hs1,2 Enhancer Within The 3' Immunoglobulin Heavy Chain Regulatory Region, Ruth C. Chambers-Turner

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2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a disrupter, of B-cell differentiation, induces binding of the aryl hydrocarbon receptor (AhR) nuclear complex to dioxin responsive elements (DRE) within the mouse immunoglobulin heavy chain regulatory region (3'IgHRR), and produces a marked inhibition of 3'IgHRR activation, IgH expression, and antibody secretion in a well-characterized mouse B-cell line (CH12.LX). The mouse 3'IgHRR consists of at least four enhancers (hs3a; hs1,2; hs3b; hs4), and is highly homologous with the three enhancers (hs3; hs1,2; hs4) of the human 3'IgHRR. A polymorphism of the human hs1,2 enhancer (resulting in varying numbers of tandem repeats containing a DRE and κB site) has …

Rev Interacts With Tubulin Heterodimers To Cause Cell Cycle Defects, Poornima Kotha Lakshmi Narayan

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Rev is a regulatory protein that plays an important role in the replication of HIV virus by post-transcriptionally promoting expression of viral proteins late in infection. Rev expression also slows cell growth, leads to an accumulation of cells in G2/M specifically before the spindle checkpoint, and can produce changes in ploidy. Because Rev is capable of depolymerizing microtubules (MTs) in vitro, possibly by a mechanism shared with Kinesin-13 proteins, themselves potent cellular MT depolymerases, I tested the hypothesis that these cellular defects were due to an interaction between Rev and tubulin.

To this end, Rev and select Rev mutants defective …

Selected Comparison Of Global Health Organizations, Steven C. Mccullar

Scholarship in Medicine - All Papers

Due to a potential, worldwide adverse impact on health, transnational dependencies, and the need for effective response, global cooperation is imperative (Buchanan & Decamp, 2006). There are more than 100 global health agencies, including the World Health Organization. As the number of organizations increases, each with their own agendas, so does the concern for lack of coherence and collaboration among organizations in the effort of disease eradication and development of health systems (Beaglehole & Bonita, 2008). The focus of this study is on comparing the effectiveness of selected global health organizations in efforts to detect a need to either establish …

Direct To Consumer Drug Advertisements: A Dangerous Game Of Pitching Products To Parents, Lynne Graziano

Annual Conference Presentations, Papers, and Posters

The annual budget for drug advertising in the United States exceeds that for all undergraduate and postgraduate medical education and comes close to the entire budget of the National Institutes of Health. In 2008 drug advertisers spent $4.7 billion, down from a record $4.8 billion in 2006, but up from $2.6 billion in 2002. Since the relaxation of full disclosure rules for television advertising, the amount spent on direct-to-consumer advertisements (DTCA) has soared, leading to increased sales of certain pharmaceuticals, as advertisements are placed in magazines, newspapers, internet websites and on television. These prescription-drug ads prompt nearly one-third of Americans …

Vital Signs, Spring/Summer 2009, Boonshoft School Of Medicine

Vital Signs

A fifty-six page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles and smaller pieces that focus on faculty, students, milestones and more.

The Equation, Spring 2009, College Of Science And Mathematics, Wright State University

College of Science and Mathematics Newsletters

This 8 page newsletter discusses various happenings within the College of Science and Mathematics. It begins with a letter from the dean, and continues on with news, events, alumni news, and other community news.

Periodic Traveling Waves In Sirs Endemic Models, Tong Li, Yi Li, Herbert W. Hethcote

Mathematics and Statistics Faculty Publications

Mathematical models are used to determine if infection wave fronts could occur by traveling geographically in a loop around a region or continent. These infection wave fronts arise by Hopf bifurcation for some spatial models for infectious disease transmission with distributed-contacts. Periodic traveling waves are shown to exist for the spatial analog of the SIRS endemic model, in which the temporary immunity is described by a delay, but they do not exist in a similar spatial SIRS endemic model without a delay. Specifically, we found that the ratio of the delay ω in the recovered class and the average infectious …

Smoking And Second Hand Smoking In Adolescents With Chronic Kidney Disease: A Report From The Chronic Kidney Disease In Children (Ckid) Cohort Study, Abiodun Aderogba Omoloja, J. Jerry-Fluker, Alison Abraham, S. Furth, B. Warady, M. Mitsnefes

Pediatrics Faculty Publications

The goal of this study was to determine the prevalence of smoking and second hand smoking [SHS] in adolescents with CKD and their relationship to baseline parameters at enrollment in the CKiD, observational cohort study of 600 children (aged 1-16 yrs) with Schwartz estimated GFR of 30-90 ml/min/1.73m2. 239 adolescents had self-report survey data on smoking and SHS exposure: 21 [9%] subjects had “ever” smoked a cigarette. Among them, 4 were current and 17 were former smokers. Hypertension was more prevalent in those that had “ever” smoked a cigarette (42%) compared to non-smokers (9%), p

In-Vitro Biocompatibility Of Silver Nanoparticles Anchored On Multi-Walled Carbon Nanotubes, Alicia Brooks Castle

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Silver (Ag) nanoparticles were anchored on the surface of three different multi-walled carbon nanotubes (MWNTs): Ag-MWNTs (pure carbon), Ag-MWNT-COx (carbonyl and carboxyl doped), and Ag-MWNT-CNx (Nitrogen-doped). The chemical reactivity of the nanotubes was MWNTs< COx-MWNTs< CNx-MWNT. Ag-NP anchored on the MWNTs had an average size corresponding to MWNTs 6.64nm +/- 2.25nm; CNx-MWNTs, 13.24nm +/- 3.94nm, and COx-MWNT/Ag 11.75nm +/- 4.65nm. Cellular function and immune response were evaluated to determine biocompatibility of the synthesized nanomaterials (NM) on the human keratinocyte cell line (HaCaT). Cellular assays revealed toxicity after 24h however, full cellular recovery was observed at 48h. These results suggest Ag nanoparticles anchored on MWNTs have the ability to interact with cells without disrupting cellular function.

Host Defense Mechanisms Against Mycobacterium Tuberculosis, Laurin Nicole Holloway

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Mycobacterium tuberculosis is one of the world's most infectious diseases. Approximately 2 million people die each year from Mycobacterium tuberculosis infection and one-third of the world's population remains infected. For decades research has focused on uncovering the tactics used by Mycobacterium tuberculosis to evade host immune responses and defense mechanisms used to fight tuberculosis infection. The following review focuses on the host defense mechanisms used to combat Mycobacterium tuberculosis with an emphasis placed on the roles that Toll-like receptors (TLRs), T cells (gamma delta, CD4, CD8), and macrophages play in mounting the innate and adaptive immune responses necessary to eradicate …

Viral Vectors In The Research Laboratory: Just How Safe Are They?, Dawn P. Wooley, Kimberly Kay Morris, Robert Mcrae, John C. Trefry

Neuroscience, Cell Biology & Physiology Faculty Publications

No abstract provided.

Flight Physician - July, 2008, Civil Aviation Medical Association

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A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Wsu Research News, Spring 2008, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A sixteen page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.