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The Effects Of Hsv-1 Challenge On Polarized Murine Macrophages: An In Vitro Model Using The J774a.1 Murine Macrophage Cell Line, Adam Craig Reichard

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In our current study we examined the effects of HSV-1 challenge on J774A.1 macrophages polarized to either a proinflammatory (M1) or anti-inflammatory (M2) phenotype. Polarized J774A.1 macrophages were characterized using CD14-CD86 and SOCS1-SOCS3 expression levels. SOCS proteins are a family of proteins that are capable of inhibiting cytokine-signaling pathways. HSV-1 up regulates expression of SOCS1 protein levels in infected cells, inhibiting the ability of infected cells to produce proinflammatory products (Nowoslawski Akhtar and Benveniste, 2011). This study shows that signals within the microenvironment play a greater role in macrophage polarization, and SOCS1-SOCS3 expression levels, than does HSV-1 challenge. M1 macrophages …

Suppressor Of Cytokine Signaling (Socs) 1 & 3 Expression In Hsv-1- Infected And Interferon-Γ-Treated Neuro-2a Cells, Melinda Jones

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This study examined the effects of HSV-1 infection and IFN-γ treatment on Neuro-2A cells. HSV-1 induces expression of SOCS1 and SOCS3 in infected cells, inhibiting the ability of these cells to produce the pro inflammatory, antiviral cytokine IFN-γ (Nowoslawski and Benveniste, 2011). SOCS1 and SOCS3 levels were determined in IFN-γ-treated cells, virus-infected cells, and cells that were both IFN-γ-treated and virus-infected. Results were compared with untreated, uninfected control cells. Flow cytometry data analysis showed a slight decrease in SOCS1 and SOCS3 protein levels in cells treated with IFN-γ for 6 hours compared to control cells. A significant decrease in SOCS1 …

The Construction Of A Plasmid For Detecting The Pathway Of Arginine Metabolism In Human Macrophages: A Real-Time Assessment Of Macrophage Polarity, Benjamin A. Holmes

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Macrophages are "first responders", innate immune system cells which quickly arrive to a site of infection and injury, consuming cell debris and foreign matter and recruiting other immune system cells to the area. While historically they have been thought to react uniformly to all challenges, the discovery of toll-like receptors has shown that macrophages actually work closely with the adaptive immune system in fine-tuning the immune response. Furthermore, it has recently been discovered that macrophages can become polarized to one of two subtypes-M1 or M2.

M1 macrophages are efficient producers of reactive oxygen species, nitrogen intermediates, and inflammatory cytokines. They …

Obesity: The Anthropometric And Demographic Characteristics Of Insured And Uninsured Individuals, Terra Fox Williams

Master of Public Health Program Student Publications

Objectives. This study evaluates the association between Body Mass Index (BMI) and health insurance status in attendees at a local health fair. BMI was also compared among ages, gender, and races.

Methods. Descriptive analysis conducted using data collected at the “Celebrating Life and Health” health fairs between the years 2008 and 2011. Data for this study were collected at the BMI booth staffed by Wright State Boonshoft School of Medicine students.

Results. The results of this particular study do not demonstrate a statistical difference between obesity and health insurance status. Consistent with other research articles this study does show that …

The Role Of Supermarkets In Community Nutrition, Staci Gruber

Master of Public Health Program Student Publications

Background: Chronic diseases related to nutrition are a serious threat to public health. Supermarkets offer an important and promising venue to improve diet quality and overall health. The purpose of this study is to evaluate the supermarkets’ community nutrition focus and interest in public health.

Methods: This study emphasis’s a triangulated qualitative study design. It employs various methods and taps various sources for data. Twenty face-to-face, semi-structured key informant interviews were conducted with five full service supermarket chains in Montgomery County, Ohio; Wal-Mart, Kroger, Meijer, Cub Foods, and Dorothy Lane Market. Qualitative sources of information collected include store observation and …

Are Student Nurses Ready For Disasters? An Analysis Of Emergency Preparedness Content In Nursing School Textbooks Used In The Miami Valley, Kimberly Caudill

Master of Public Health Program Student Publications

Results of an analysis of nursing textbooks used in the Miami Valley, indicate that there is minimal emergency preparedness content included. The research was based on the textbooks used in the Introduction to Nursing, Psychiatric Nursing, and Community Health Nursing classes at four local nursing schools: Sinclair Community College, Kettering College of Medical Arts, Cedarville University, and Wright State University. The Community Health Nursing classes have the most prepared content, and include extra content in the form of tables and pictures .The analysis indicates that Wright State University does the best job at preparing student nurses to be ready to …

Wsu Research News, Fall 2011, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A nineteen page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Assessment Of Refugees’ Health In Montgomery County: A Case Study Of Refugees From Rwanda And Burundi, Jean De Dieu Mukunzi

Master of Public Health Program Student Publications

The purpose of this research study was to identify health needs and health problems experienced by a refugee/asylee population in Montgomery County. Montgomery County hosts a large number of refugees, asylees and asylum seekers from countries such as Iraq, Ethiopia, Eritrea, Soudan, Rwanda, Burundi and Congo.

A public health challenge is limited demographic data about this population. The scarce data on their health needs slows down not only their successful resettlement but also limits community organizations, health and human services agencies to improve the delivery of health care services and health promotion programs to refugees and immigrants in Montgomery County. …

Flight Physician - August, 2011, Civil Aviation Medical Association

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A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

The Non-Infectious Disease Implications Of Bed Bug Infestations, Christopher T. Balster

Master of Public Health Program Student Publications

The purpose of this study was to determine the prevalence of bed bug infestations throughout Ohio, whether the non-infectious disease implications of infestations are public health concerns, and also determine the perceived ability of public health departments to respond to outbreaks within their jurisdiction. A descriptive study was performed using survey data obtained from 79 local public health departments throughout Ohio. Overall, 79.05% of individuals surveyed agreed that bed bug outbreaks are a public health concern. The mode of responses regarding whether or not the non-infectious disease concerns of infestations are a public health concern was 4 on a scale …

College Of Science And Mathematics Annual Report 2011, College Of Science And Mathematics, Wright State University

College of Science and Mathematics Annual Reports

This 18 page report summarizes the yearly happenings within the College of Science and Mathematics. It includes information on events through the year, enrollment, budget, and other important events within the college.

Wsu Research News, Winter/Spring 2011, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A twenty-four page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Utilization Of A Custom-Designed Microbiota Array To Determine The Distal Gut Microbiota Of Healthy Human Adults, Richard Thomas Agans

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The human microbiota is an essential component of human health and disease. It is involved in metabolism of dietary components and is at the forefront of the intestinal immune response. Classical techniques applied to study intestinal microbiota illustrated higher presences of aerobic and facultative-anaerobic bacteria, however; the levels of obligate anaerobes had been underrepresented. Modern technologies based on DNA and RNA analysis have circumvented previous challenges allowing researchers to gain more extensive insight into the complex intestinal environment. In this work, a recently developed Microbiota Array was used to assess intestinal microbiota of 10 healthy adults (age 22-61, ave. 34.3yrs). …

Nation's Future: Preparedness For Burn Victims, Jacqueline Tippett

Master of Public Health Program Student Publications

Natural and manmade disasters have occurred throughout the decades, within the United States and around the globe. Increased incidence and awareness of terrorist attacks have caused the US to re-evaluate its safety and preparedness efforts to prevent such events from happening and to develop an adequate response plan if such unfortunate events do occur. Therefore, the purpose of this research is to (1) review and discuss the American Burn Association’s (ABA) paradigm for emergency planning in burn disasters, (2) give recommendation about the National Response Framework (NRF) guidelines on “all hazards” response as they pertains to burns, and (3) give …

Acl Injuries In Montgomery County, Oh: Moving Toward The Development Of A Prediction Model, Kelly Estes

Master of Public Health Program Student Publications

Anterior cruciate ligament (ACL) tears are one of the most frequent soft tissue injuries of the knee. A torn ACL leaves the knee joint unstable and at risk for further damage to other soft tissues manifested as pain, dislocation, and osteoarthritis. A better understanding of the dimensional details of knee joints suffering ACL tears and a prediction model for individuals susceptible to tears is needed. Using a cross-sectional study design, magnetic resonance images (MRIs) of 72 patients with knee injuries were evaluated from an orthopedic surgery group practice. The status of the ACL was the main variable of interest, creating …

State Preparedness: A Study Of State Plans, Zachary Fehrman

Master of Public Health Program Student Publications

Non-pharmaceutical interventions (NPIs) have been used since the early years of public health. The Centers for Disease Control and Prevention (CDC) provides a standard definition for NPI and what NPI strategies are contained within the definition. This paper seeks to discover if state pandemic influenza plans use the term NPI and its strategies, and if states’ use of NPI strategies in their state pandemic plans is consistent with the CDC definition. To determine how states were defining and using the term NPI and NPI strategies a survey of forty-eight (48) state plans was conducted using each respective states’ pandemic plan. …

Prevalence, Knowledge, And Concern About Bed Bugs, Mary Beth Kaylor

Master of Public Health Program Student Publications

Recent research suggests that the United States and the world are on the verge of a bed bug pandemic. Assumptions have been made that socioeconomic status is not an indicator and that bed bugs are not competent disease vectors. However, little information is available at the local level about the prevalence of bed bugs in private homes. This study aimed to identify prevalence, knowledge, and concern about bed bugs in one village in Ohio. Responses from 96 individuals who completed the Prevalence, Knowledge, and Concern about Bed Bugs (PK CABB) survey were utilized for data analysis. The majority of the …

Situation Analysis Of Disability Resources And Needs Of Shantytowns Near Lima, Peru, Jessica Hunt

Master of Public Health Program Student Publications

Individuals in shantytowns of Lima, Peru experience unique challenges due to socioeconomic status and perceived inabilities. A situation analysis using qualitative ethnographic interviews of key participants (n=15) and one focus group (n=7) was conducted to explore life with disability in a shantytown of Lima, Peru. Findings showed that individuals with disability face physical and attitudinal barriers that limit social inclusion through the avenues of rehabilitation, education, and employment. The familial complex is expected to provide care for the individual with disability. The adult family member often chooses between providing income for the household and providing care and safety for their …

Integrating Aircrew Resources Variability Into The Design Of Future Cockpit, Barbara Mawhin, Philippe Cabon, Florence Buratto

International Symposium on Aviation Psychology - 2011

Cockpit design may have major consequences on future pilot’s tasks. Functions needed to ensure flight safety are shared between pilots and aircraft systems. Today, cockpits are designed considering a theoretical minimum level of crew resources availability although it is widely acknowledged that availability of crew resources may vary from time to time because of aircrew internal state. Therefore, it is crucial to ensure that future cockpits are designed while taking into consideration this variability. This work aims at developing a methodology enabling designer to systematically integrate crew resources availability in the design process. In order to assess the impact of …

The Effect Of Prenatal Care And Other Factors On Preterm Births In Montgomery County 2007-2009, Leslie A. Schmieder

Master of Public Health Program Student Publications

Introduction Preterm birth is defined as a baby who is born before 37 weeks of pregnancy. Problems with preterm birth include an increased risk for health complications throughout life as well as having significantly more medical expenses compared to a full term infant. Prenatal care has shown to decrease the risk for preterm birth nationally. The objective of this project was to analyze prenatal care and other risk and demographic factors to determine their association with preterm birth in Montgomery County.

Method Data used included the 2007-2009 Montgomery County Birth Certificate Data for mothers who had singleton births. The Kessner …

Population Pharmacokinetic (Pk) And Pharmacodynamic (Pd) Modeling Of Ticarcillin‐Clavulanate In Pediatric Cf Patients, Jeffery T. Zobell, Chris Stockmann, D. C. Young, Jared Cash, Catherine M.T. Sherwin, Michael G. Spigarelli, Barbara A. Chatfield, Krow Ampofo

Pediatrics Faculty Publications

No abstract provided.

Ethical Issues In Global Health Education, David Barnard, T. Thuy Bui, Jack Chase, Evaleen Jones, Scott Loeliger, Anvar Velji, Mary T. White

Population and Public Health Sciences Faculty Publications

No abstract provided.

Pertussis In Ohio: A Descriptive Analysis Of The 2010 Columbus Outbreak And Patterns Of A Reemerging Childhood Disease, Christopher Rohde

Master of Public Health Program Student Publications

Objective: In 2010, Columbus, Ohio experienced the largest outbreak of pertussis that it has had in the past 25 years. Similar outbreaks are occurring across the nation on an increasing basis. This descriptive study examines the 2010 outbreak and describes the demographics of the affected population. Comparison is made to previous and current pertussis incidence in Ohio and outbreaks elsewhere in the United States. Contributing factors to the increasing occurrence of pertussis outbreaks are discussed.

Methods: Raw data was obtained from the Columbus Public Health epidemiology department and is gathered through the Ohio Disease Reporting System (ODRS). Pertussis cases are …

Gaps And Strengths In Us Policies On Infant And Young Child Feeding: An Analysis Based On The Global Strategy On Infant And Young Child Feeding, Linda J. Smith

Master of Public Health Program Student Publications

This research project reviews the evidence on the significance of infant and young child feeding to public health goals, analyzes US policy documents in relationship to the Global Strategy using the World Breastfeeding Trends Initiative (WBTi) to quantify the findings into a national score, and then summarizes the gaps and strengths in US policies and makes recommendations. Using the WBTi scoring system, strengths for the US exist in national policy, Baby-Friendly Hospital Initiative, monitoring and evaluation, and complementary feeding. Mother support and community outreach, information and support were moderate. Gaps exist in policies on infant feeding in emergencies and for …

Determining Evidence-Based Practices In Asthma Management, Erin Smiley

Master of Public Health Program Student Publications

Objective: One aim of this study will focus on determining what are evidence-based studies in asthma control. A second aim focuses on determining which asthma interventions, self-monitoring tools and asthma management programs have shown to be the most significant in improving outcomes in patients with asthma. Recommendations will be made to future asthma self-management programs based on the findings.

Methodology: The review of 15 evidence-based research studies is used to identify and analyze the best asthma self-management practices to be implemented in a community health education program. Using the Brownson framework a weighting schema was created based on the level …

A Validation Of A Prototype Dry Electrode System For Electroencephalography, Jason Monnin

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Current physiologically-driven operator cognitive state assessment technology relies primarily on electroencephalographic (EEG) signals. Traditionally, gel-based electrodes have been used; however, the application of gel-based electrodes on the scalp requires expertise and a considerable amount of preparation time. Additionally, discomfort can occur from the abrasion of the scalp during preparation, and the electrolyte will also begin to dry out over extended periods of time. These drawbacks have hindered the transition of operator state assessment technology into an operational environment. QUASAR, Inc., (San Diego, CA) has developed a prototype dry electrode system for electroencephalography that requires minimal preparation. A comparison of the …

Hsv-1 Infection In Keratinocyte Cell Lines Treated With Mitotic Inhibitors, Asma A. Abbas

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The hypothesis for this research was: Herpes simplex virus type 1 (HSV-1) infection of murine keratinocyte cell lines (HEL-30 and PAM-212) treated with mitotic inhibitors leads to silencing of virus replication. At 0.1 (Multiplicity of infection) MOI, PAM-212 keratinocytes showed some cell lysis and viral plaques were seen in HEL-30 keratinocyte cultures. PAM-212 keratinocytes, infected at 0.01 MOI, were more susceptible to the lytic effect of HSV-1 than were HEL-30 cells. In this study, 5-fluoro-2'-deoxyuridine (FUDR) treatment of both keratinocyte cell lines permitted an increase survival of HSV-1- infected keratinocytes especially for PAM-212 cell line which was further examined for …

Distribution Of Cellular Interferon Beta (Ifn-Β) In Murine Fibroblast Cell Lines Upon Infection Of Hsv-1, Rachael E. Curtis

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The hypothesis for this study was: IFN-β expression differs between murine fibroblasts (A2R1 and L929) of different mouse strains upon infection of HSV-1. Fluorescent microscopy was used to examine localization of IFN-β in the different murine fibroblast and keratinocyte cell lines after 6 hours of infection with herpes simplex virus type-1 (HSV-1). Because keratinocytes cell lines HEL-30 and PAM-212 grew in clusters, staining patterns in individual cells could not be determined. A notable difference in localization of IFN-β immune staining was seen between the two cell lines when infected with HSV-1. The A2R1 cell line showed perinuclear (PN) localization, while …

Targeted Knockdown Of Amp-Activated Protein Kinase Alpha 1 And Alpha 2 Catalytic Subunits, Larissa J. Tangeman

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AMP-activated protein kinase (AMPK) regulates cellular metabolism and promotes ATP production when energy is depleted. Evidence suggests that AMPK may be involved in oxygen sensing by carotid body cells, which are responsible for regulating the breathing rate to maintain proper blood oxygen levels. There are two isoforms of the catalytic alpha subunit, AMPKalpha1 and AMPKalpha2, which could be involved in oxygen sensing. Here, the production of a short hairpin RNA (shRNA) targeting both catalytic isoforms of AMPK in human, mouse, and rat is described. The shRNA causes significant knockdown of both isoforms of AMPK alpha in mouse and human cells …

Strategies For Long-Term Renal Allograft Survival In Iga Nephropathy Patients, Erin E. Lintz

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IgA nephropathy is one of the most common kidney diseases worldwide. Most IgA nephropathy patients will undergo a kidney transplant as a treatment. Treatment currently includes the use of immunosuppressants which are necessary to prevent graft rejection but present harmful side-effects when taken long-term. This review focuses on strategies which have the ability to promote tolerance of both donor and recipient cells within a transplant recipient. Such strategies allow development of mixed chimerism and thereby alleviate the need for long-term immunosuppressant usage in allograft recipients. These strategies could be applied to IgA nephropathy patients to allow kidney allograft acceptance without …