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An Examination Of Cyanotoxins And Environmental Estrogens In The Middle And Upper Potomac River Watershed, Usa, Ryan Patrick Braham

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Potomac River is a large tributary of the Chesapeake Bay. Large fish die-offs were reported in the Potomac watershed (specifically the South Branch of the Potomac) in 2002 and 2005, as well as smaller episodic events in recent years which have no clear correlation to any one pathogen or contaminant. Although a variety of pathogens have been identified (such as trematodes, myxozoans, Aeromonas hydrophila, A. salmonicida, Flavobacterium columnare , and largemouth bass virus) in recent die-offs of smallmouth bass Micropterus dolomieu, no clear cause has been derived. Additionally, reproductive endpoints were reported in smallmouth bass and likely associated with …

Remotely Sensed Hydroclimatic Variability For Water Security In An Agricultural Basin, Ashish Kondal

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Indus Basin has supported agricultural practices since the Harappan civilization (3000-1500 BC). Abundance of water has always supported agriculture in the region and built the Indus Basin as one of the major agriculture based economies of the world. The obvious dependency of agriculture on weather and the vagaries of climate emphasizes the necessity of a comprehensive hydro-climate study of the region. The role of climate change in the Indus Basin has always been a contentious issue. Groundwater depletion is evident in the region, and its steady and alarmingly declining rate has brought concerns for the future of agriculture in …

Effects Of Pre-Breeding Management On Ewe Lamb Fertility, Sarah Nancy Carr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ewe lambs comprise 30% of the breeding flock; however, fertility is 20-30% lower than that of an adult ewe. Lower fertility has been correlated with lower ADG, liveweight, and body composition prior to breeding and research suggests that it can be improved with proper nutritional management. The objective of this research was to evaluate the effects of pre-breeding management practices on ewe lamb fertility.;In replicate 1, 313 Dorset X Texel (DT) and 74 Katahdin (KT) ewe lambs were assigned to a high (0.68 kg/head/day) or low (0.23 kg/head/day) grain supplementation for two months prior to breeding. Liveweights were recorded bi-weekly …

Exploring The Use Of Sport As A Platform For Health Promotion With Youth In Africa: A Scoping Review., Adam H. Hansell

Graduate Theses, Dissertations, and Problem Reports (ETD)

According to the World Health Organization, Africa has the highest rates of disease and child mortality in the world. Previous research suggests that sport may be an effective vehicle to enhance health knowledge and behaviors among at-risk youth. The primary purpose of this review was to analyze and synthesize published interventions exploring the use of sport or physical activity for health promotion with children and youth in Africa. A total of 916 articles were retrieved from ten electronic bibliographic databases with 28 meeting inclusion criteria. Targeted health outcomes in sport-based interventions included HIV-related knowledge and behaviors, essential health practices, physical …

Chitosan Biopolymer Promotes Yield And Stimulates Accumulation Of Antioxidants In Strawberry Fruit, Mosaddiqur Rahman, Julakha Akter Mukta, Abdullah As Sabir, Dipali Rani Gupta, Mohammed Mohi-Ud-Din, Mirza Hasanuzzaman, Md. Giashuddin Miah, Mahfuzur Rahman, Md Tofazzal Islam

Faculty & Staff Scholarship

Strawberry is a well-known source of natural antioxidants with excellent free radical scavenging capacity. This study determined the effects of chitosan application in field condition on plant growth, fruit yield and antioxidant activities in strawberry fruit. Foliar applications of chitosan on strawberry significantly increased plant growth and fruit yield (up to 42% higher) compared to untreated control. Increased fruit yield was attributed to higher plant growth, individual fruit weight and total fruit weight/plant due to the chitosan application. Surprisingly, the fruit from plants sprayed with chitosan also had significantly higher contents (up to 2.6-fold) of carotenoids, anthocyanins, flavonoids and phenolics …

Application Of Crispr/Cas9 Genome Editing Technology For The Improvement Of Crops Cultivated In Tropical Climates: Recent Progress, Prospects, And Challenges, Effi Haque, Hiroaki Taniguchi, Md. Mahmudul Hassan, Pankaj Bhowmik, M. Rezaul Karim, Magdalena Śmiech, Kaijun Zhao, Mahfuzur Rahman, Tofazzal Islam

Faculty & Staff Scholarship

The world population is expected to increase from 7.3 to 9.7 billion by 2050. Pest outbreak and increased abiotic stresses due to climate change pose a high risk to tropical crop production. Although conventional breeding techniques have significantly increased crop production and yield, new approaches are required to further improve crop production in order to meet the global growing demand for food. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 (CRISPR-associated protein9) genome editing technology has shown great promise for quickly addressing emerging challenges in agriculture. It can be used to precisely modify genome sequence of any organism including plants …

An Experimental Investigation Of The Banana Dehydration Process., Donnique Kim Denise Sherman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drying food is one of the well-known methods of food preservation. Food preservation methods, like drying, slow down the deterioration of foods as well as prevents the growth of microorganisms. Food preservation allows for an extension of shelf life and prevents food from spoiling. The method used in this study is known as convective or direct drying. As the ambient air heats up from a convective source the relative humidity reduces which accelerates the drying force and decreases drying time. The reduction in relative humidity allows for moisture to move from the food, such as banana chips, to the air. …

Morphological, Genetic And Biochemical Characterization Of The Anti-Malarial Herb, Artemisia Annua Germplasm Collection At West Virginia University, Delini K. Samarasinghe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malaria is one of the deadliest diseases in human history. Nearly half of the world’s population, is at the risk in 106 countries. Only in 2016, this disease killed about 445,000 people, 72% of them being children under age five. It also accounts for US $12 billion dollars of direct costs in Africa alone. Five different species of Plasmodium cause malaria but P. falciparum is the most detrimental one, causing 50% of all malaria cases and is considered as the deadliest parasite in humans. Artemisinin (ART), a 15 C sesquiterpenoid is currently the only precursor to the most effective anti-malarial …

Association Of Diet Quality And Body Mass Index With Serum Ceramides In Middle-Aged Adults, Margaret Ann Drazba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cardiovascular disease (CVD) is one of the leading causes of death worldwide and identifying CVD risk is important for determining and implementing appropriate intervention strategies. Ceramides are a class of sphingolipids that are implicated in the pathogenesis of obesity and CVD. However, their relationship to diet quality, a modifiable risk factor for CVD, is largely unknown. The purpose of this cross-sectional study was to determine the association of an a priori diet quality index and body mass index (BMI) with circulating ceramides in middle-aged adults (n=96). Dietary intake was assessed using three 24-hour recalls and Nutrition Data System for Research …

Protein And Lipid Characterization Of Acheta Domesticus, Bombyx Mori, And Locusta Migratoria Dry Flours, Emily N. Brogan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cricket (Acheta domesticus), silkworm pupae (Bombyx mori), and locust (Locusta migratoria) dry flours were obtained to examine the biochemical properties and composition of the flours. This study aimed to characterize the protein and lipid components of three insect species utilizing proximate composition analyses, amino acid composition analysis, protein solubility, SDS-PAGE, fatty acid composition analysis, and thin layer chromatography to determine lipid classes, and lipid extraction efficiency. Kjeldahl determined the cricket, locust, and silkworm flours contained 72.0%, 53.1%, and 71.2% protein, respectively. All proximate composition analyses were significantly (p< 0.05) different between species. Amino acid composition analysis revealed that the flours contained 3.6-3.9% and 0.90-1.5% of lysine and methionine, respectively. Many countries have cereal grains and legumes as a staple in their diet that contain these limiting amino acids. Essential amino acids were 22% of total amino acids. Protein solubility revealed that the three species studied were most soluble in alkaline environments with highest protein solubility occurring at pH 13 at 66% solubility in silkworm. Lowest solubility occurred in more acidic conditions between pH 4-5. SDS-PAGE revealed five major protein fractions with estimated molecular weights of 27 (cuticle proteins), 41 (arginine kinase), which has been identified as an allergen in shrimp, 42 (actin), 71 (hemocyanin), and 220 myosin) kDa. Soxhlet extraction determined cricket, silkworm, and locust flours contained 15.4%, 33.3%, and 11.4% lipid, respectively. The only omega-3 fatty acid found in the flours was α-linolenic acid. Silkworm flour contained the most α-linolenic acid at 33.3% of total fatty acids, followed by locust (13.7%), and cricket (0.6%). TLC resolved lipids for typical lipid classes. The four major lipid classes noted were triacylglycerol, free fatty acid, cholesterol, and phospholipid. Lipid extraction efficiency found that the organic solvents chloroform and methanol had the highest lipid extraction yield in both cricket (69.3%) and locust (93.0%). Methyl-tertiary-butyl ether (MTBE) extracted lipids most efficiently in the silkworm. Proteins and lipids found in insects need to be isolated to further or commercial use.

The Impact Of Comorbidities On Diabetes And Hypertension Co-Management And Healthcare Expenditures, Rashmi Goyat

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hypertension and type-2 diabetes mellitus (T2DM) are among the most prevalent chronic conditions that often co-exist. Their co-existence increases the risk for comorbidities due to their impact on other organs such as blood vessels (leading to vasoconstriction/hypertension); brain (resulting in increased caloric intake); pancreas (decreased in beta-cell mass and glucose sensing); bone (possibly decreasing bone mass and strength). Occurrence of comorbidities can complicate the already complex care of diabetes and hypertension. It increases the risk of poor patient outcomes such as preventable hospitalization, adverse drug events and mortality. However, different comorbidities can affect the co-management differently. We identified the types …

Prenatal Lactation-Focused Motivational Interviewing For Enhancing Breastfeeding Initiation, Exclusivity, And Duration: Feasibility And Preliminary Outcomes, Sarah H. Addicks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breastfeeding promotes health and well-being for both mother and infant. A variety of environmental and individual factors, including psychological ones, affect infant feeding practices. The purpose of this study was to determine the feasibility and effectiveness of a single-session Motivational Interviewing (MI) intervention delivered during the third trimester of pregnancy for enhancing breastfeeding outcomes. The Theory of Planned Behavior provided a theoretical framework for the intervention and outcome measures. The sample consisted of predominantly rural participants living in the North Central Appalachian region. Women were recruited through social media, local clinics, and word of mouth. Participants (N = 81) completed …

Investigation Into Composting Efforts At Wvu: A Case Study., Marina Alexis Berry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Institutional composting systems have been adopted by many land grant universities to help better manage their waste and protect the environment. West Virginia University (WVU) has yet to begin formally managing their animal manure and waste at the university farms. This investigation will look into what has been done at other land grant universities to see which methods could be applied at WVU. This could have environmental, economic, and social benefits and also serve as a living learning classroom. University composting systems have often started small and grown to accommodate campus food waste, which could also be a possibility for …

Plant Probiotic Bacteria Bacillus And Paraburkholderia Improve Growth, Yield And Content Of Antioxidants In Strawberry Fruit, Mosaddiqur Rahman, Abdullah As Sabir, Julakha Akter Mukrta, Mohibul Alam Khan, Mohammed Mohi-Ud-Din, Giashuddin Miah, Mahfuzur Rahman, M. Tofazzal Islam

Faculty & Staff Scholarship

Strawberry is an excellent source of natural antioxidants with high capacity of scavenging free radicals.

This study evaluated the effects of two plant probiotic bacteria, Bacillus amylolequefaciens BChi1 and Paraburkholderia fungorum BRRh-4 on growth, fruit yield and antioxidant contents in strawberry fruits. Root dipping of seedlings (plug plants) followed by spray applications of both probiotic bacteria in the field on foliage significantly increased fruit yield (up to 48%) over non-treated control. Enhanced fruit yield likely to be linked with higher root and shoot growth, individual and total fruit weight/plant and production of phytohormone by the probiotic bacteria applied on plants. …

Imidacloprid Bark Treatment For Hemlock Woolly Adelgid (Adelges Tsugae), Matthew D Quinterno

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Examining Participant Perceptions Of Benefits, Barriers And Social Support In A Diabetes Lifestyle Program, Samantha Shawley-Brzoska

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

An Examination Of Cardiovascular Health Among Afro-Caribbean Immigrants In New York City, Sabena C Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Determination Of The Consensus Target Sequences Recognized By Znfo, A Novel Oocyte-Specific Zinc Finger Transcription Factor In Cattle, Mingxiang Zhang

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Gaining Consensus On Implementation, Sustainability, And Benefits Of School Garden Programming In Washington, D.C., Hannah J Kipfer

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

"Squeezed Between The Gunshots And The Gentrifiers”: Urban Agriculture In Philadelphia's Kensington Neighborhood, Arianna Hall-Reinhard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban agriculture (UA) is part of the broader alternative food movement and a potential avenue through which to “do” food justice work. UA projects in the urban Global North are frequently motivated by social and food justice goals. Despite these guiding ideals, UA projects in America are rife with internal contradictions, including those related to racial inequalities, complex gentrification dynamics, and funding realities. In this paper, I employ the conceptual frameworks of food justice and urban political ecology to consider how gentrification and UA project funding structures affect five specific UA projects in Philadelphia’s Kensington neighborhood. While the loss of …

Automated Analysis Of Polypharmacy Using Prescription Drug Claims Data, Shahad Nagoor

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Antibacterial Activity Of Current Endodontic Repair Materials Against Enterococcus Faecalis., Michael A. Tran

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Root canal perforations caused by pathologic or iatrogenic means have been shown to cause serious complications leading to overall tooth loss. Bacterial infection of the affected site results in failure of the root canal as well as an inflammatory response leading to the destruction of supporting tissues. Although a multitude of endodontic repair materials have been tested over the past several decades, recent introduction of bioceramic materials have shown favorable outcomes to repairs. Bioceramics not only exhibit excellent biocompatibility, but also may possess antibacterial properties due to their strong alkaline pH. The aim of this in vitro study was …

Potential Effects Of Oil And Natural Gas Development On Mule Deer (Odocoileus Hemionus) Survival And Fawn Rearing Resource Selection, Brett P. Skelly

Graduate Theses, Dissertations, and Problem Reports (ETD)

A worldwide increasing demand for both renewable and non-renewable energy resources has been ongoing for the past 50 years and is projected to continually increase for the next two decades. The direct and indirect effects of oil and natural gas development are not quantified but may be playing an important role in mule deer population dynamics. For this project I: (1) evaluated the potential effects of oil and natural gas development on survival probabilities of mule deer and; (2) evaluated the potential effects of oil and natural gas development on fawn rearing resource selection. I assessed mule deer survival and …

Predicting Student Success In Online Physical Education, Tyler Goad

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background/Purpose: Scholars have posited that the demand for online learning is not going away, and the question is no longer if online physical education (OLPE) is practical but rather, what are the most effective ways of administering OLPE to accommodate students (Daum & Buschner, 2012). Currently, limited data are available on student retention rates and attrition factors in OLPE courses. Several early OLPE studies (Brewer, 2001; Mosier, 2010; Ransdell et al., 2008) as well as the 2007 NASPE Initial Guidelines for Online Physical Education have suggested that certain prescreening efforts be in place prior to student enrollment in OLPE, however, …

Focus On The Child: Preventing The Intergenerational Transmission Of Eating Disorders And Dieting Behavior, Elizabeth Claydon

Graduate Theses, Dissertations, and Problem Reports (ETD)

PURPOSE: There is a paucity of research exploring individuals' recall of parental dieting behavior, engagement in "fat talk", or criticism of weight or eating behavior in childhood. This dearth of literature exists in both community samples and in clinical populations of eating disorder patients. There is also limited information on women with a history of eating disorders and their pregnancy experience or anticipation of their pregnancy experience. This dissertation focuses on the aforementioned areas in order to develop the research literature further to understand whether parental feedback (such as parental comments or parental dieting) is associated with health outcomes such …

Involvement Of Two Novel Grf-Type Zinc Finger Nodulins During Rhizobial Symbiotic Establishment In Medicago Truncatula Nodules, Lucas Gontijo Silva Maia

Graduate Theses, Dissertations, and Problem Reports (ETD)

In legumes, symbiotic nitrogen fixation (SNF) occurs through a cooperative relationship established with rhizobial diazotrophic bacteria. This association triggers the de novo development of a highly specialized organ from root, called nodule. In this dissertation, we explore the molecular functional genetics of nodulation in the model legume Medicago truncatula in the scope of knowledge. Followed by a linearization of the current knowledge about the roles of Transcription Factors in legume nodule symbiosis. In an attempt of understanding the orchestration dynamics of nodulation and processes that generate SNF. Legumes evolved novel genes related with nodule development, symbiosis establishment and nitrogen fixation, …

Appalachian Bariatric Surgery Population Descriptive Analysis, Surgical Outcomes, And Food Accessibility, Makenzie Leanne Barr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction. Overweight and obesity, diabetes, mental health issues, and lack of access to healthcare resources are frequent burdens among the Appalachian region of the United States. With morbid obesity, conventional behavioral interventions tend to fail. Bariatric surgery has been deemed the most successful treatment for morbid obesity and is performed regularly worldwide, however, the Appalachian population with the highest proportions of obesity and related co-morbidities has been poorly studied.;Aims. This dissertation aims to (1) provide a systematic review of the literature surrounding obesity and food access among Appalachian residents, (2) address the void in research of characterizing Appalachian bariatric surgery …

Relationship Between Adherence To Individual Goals Within The 5-2-1-0 Guidelines For Obesity Prevention And Number Of Pacer Laps In Adolescents, Kibum Cho, Emily Jones, Christa Lilly, Sean Bulger, Luciana Braga, Eloise Elliott

Clinical and Translational Science Institute

BACKGROUND—The purpose of this study was to evaluate the relationship between adolescents’ adherence to the 5-2-1-0 goals and the number of completed Progressive Cardiovascular Endurance Run (PACER) laps. METHODS—Participants included 1792 students aged 10 to 16 years who were randomly selected across 9 data collection periods between 2012 and 2014. The Survey of Physical Activity and Nutrition was used to measure time spent in physical activity and sedentary behavior, and dietary intake and the dependent variable was the number of PACER laps achieved. The KruskalWallis test and pairwise post hoc comparisons were conducted. RESULTS—Only 0.95% (N = 17) participants met …

Intimate Partner Violence-Related Hospitalizations In Appalachia And The Non-Appalachian United States, Danielle M. Davidov, Stephen M. Davis, Motao Zhu, Tracie O. Afifi, Melissa Kimber, Abby L. Goldstein, Nicole Pitre, Kelly K. Gurka, Carol Stocks

Clinical and Translational Science Institute

The highly rural region of Appalachia faces considerable socioeconomic disadvantage and health disparities that are recognized risk factors for intimate partner violence (IPV). The objective of this study was to estimate the rate of IPV-related hospitalizations in Appalachia and the non-Appalachian United States for 2007–2011 and compare hospitalizations in each region by clinical and sociodemographic factors. Data on IPV-related hospitalizations were extracted from the State Inpatient Databases, which are part of the Healthcare Cost and Utilization Project. Hospitalization day, year, in-hospital mortality, length of stay, average and total hospital charges, sex, age, payer, urban-rural location, income, diagnoses and procedures were …

Are Fair Weather Fans Affected By Weather? Rainfall, Habit Formation And Live Game Attendance, Qi Ge, Brad Humphreys, Kun Zhou

Economics Faculty Working Papers Series

We analyze habit formation in sports attendance utilizing rainfall as an unexpected, transitory shock to attendance costs. Using attendance data from Major League Baseball (MLB) and NOAA weather data, we analyze the impact of variation in game day weather conditions on current and future MLB attendance. The empirical strategy permits identification of both the formation and persistence of habit from exogenous weather shocks. Past adverse weather shocks increase future attendance by about 200 fans per game. Our study contributes to the literature developing empirical evidence of habit formation in the field and provides policy implications for optimal ticket pricing strategies.