Digital Commons Network^™

Implementation And Outcomes Of A Community-Based Pulmonary Rehabilitation Program In Rural Appalachia, Daniel Doyle, Chaffee Tommarello, Mike Broce, Mary Emmett, Cecil Pollard

Clinical and Translational Science Institute

Purpose: To report on the implementation and clinical outcomes of a community-based pulmonary rehabilitation program in rural Appalachia. Methods: Three rural health centers and a large referral hospital worked together to establish pulmonary rehabilitation services based on AACVPR guidelines. Each site hired at least 1 respiratory therapist. To measure clinical outcomes, a retrospective medical record study compared pre- and post-program values for the modifi ed Medical Research Council dyspnea level, 6-minute walk test (6MWT), negative inspiratory force (NIF), respiratory disease knowledge, St George Respiratory Questionnaire (SGRQ), BODE index (body mass index, airfl ow obstruction, dyspnea and exercise capacity), and smoking …

Physical Activity And Quit Motivation Moderators Of Adolescent Smoking Reduction, Melissa D. Blank, Kaitlyn A. Ferris, Aaron Metzger, Amy Gentzler, Christina Duncan, Traci Jarrett, Geri Dino

Clinical and Translational Science Institute

Objectives—We examined participant characteristics as moderators of adolescents’ smoking cessation outcomes as a function of intervention: Not-on-Tobacco (N-O-T), N-O-T with a physical activity (PA) module (N-O-T+FIT), or Brief Intervention (BI). Methods—We randomly assigned youth (N = 232) recruited from public high schools to an intervention, and measured their baseline levels of PA and motivation to quit. The number of cigarettes/day for weekdays and weekends was obtained at baseline and 3-month follow-up. Results—Across time-points, cigarette use declined for youth in N-O-T (p = .007) and N-O-T +FIT (ps < .02), but not BI (n.s.). For N-O-T+FIT youth, the steepest declines in weekday smoking occurred for those with high PA levels (p = .02). Weekend cigarette use decreased for NO-T+FIT youth with moderate-high levels of intrinsic motivation to quit (ps < .04). Conclusions—Adolescents may benefit from interventions designed to address the barriers faced during a quit attempt, including their motivation to make a change and their engagement in other healthy behaviors such as physical activity.

Concurrent Longitudinal Epr Monitoring Of Tissue Oxygenation, Acidosis, And Reducing Capacity In Mouse Xenograft Tumor Models, Andrey A. Bobko, Jason Evans, Nicholas C. Denko, Valery V. Khramtsov

Clinical and Translational Science Institute

Tissue oxygenation, extracellular acidity and tissue reducing capacity are among crucial parameters of tumor microenvironment (TME) of significant importance for tumor pathophysiology. In this paper we demonstrate the complementary application of particulate lithium octa-n-butoxy-naphthalocyanine (LiNc-BuO) and soluble nitroxide (NR) paramagnetic probes for monitoring of these TME parameters using electron paramagnetic resonance (EPR) technique. Two different types of therapeutic interventions were studied: hypothermia and systemic administration of metabolically active drug. In summary, the results demonstrate utility of EPR technique for noninvasive concurrent longitudinal monitoring of physiologically relevant chemical parameters of TME in a mouse xenograft tumor models including that under therapeutic …

Rates Of Nonsteroidal Anti-Inflammatory Drug Use In Patients With Established Cardiovascular Disease: A Retrospective, Cross-Sectional Study From Nhanes 2009- 2010, Gregory Castelli, Ashley Petrone, Jun Xiang, Carl Shrader, Dana King

Clinical and Translational Science Institute

Purpose—Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat pain, inflammation, and fever in the USA. Unfortunately, NSAIDs have been associated with an increased risk of adverse cardiovascular events, especially among NSAID users with established cardiovascular disease (CVD). In 2005, the Food and Drug Administration (FDA) released an initial warning regarding NSAID use and CVD risk, and recently, in July 2015, released an updated statement strengthening this initial warning. The purpose of this study is to evaluate the rates of NSAID use among patients with CVD following the 2005 FDA warning regarding NSAID use and increased CVD risk. Methods—This was …

Mitochondria Initiate And Regulate Sarcopenia, Stephen E. Alway, Junaith S. Mohamed, Matthew J. Myers

Clinical and Translational Science Institute

We present the hypothesis that an accumulation of dysfunctional mitochondria initiates a signaling cascade leading to motor neuron and muscle fiber death and culminating in sarcopenia. Interactions between neural and muscle cells that contain dysfunctional mitochondria exacerbate sarcopenia. Preventing sarcopenia will require identifying mitochondrial sources of dysfunction that are reversible.

Psychotropic Medication Use Among Medicare Beneficiaries Following Traumatic Brain Injury, Jennifer S. Albrecht, Daniel C. Mullins, Gordon S. Smith, Vani Rao

Clinical and Translational Science Institute

Objectives—To characterize psychotropic medication use before and after traumatic brain injury (TBI) hospitalization among older adults. A secondary objective is to determine how receipt of indicated pharmacologic treatment for anxiety and post-traumatic stress disorder (PTSD) differs following TBI. Design—Retrospective cohort Setting—United States Participants—Medicare beneficiaries age ≥65 hospitalized with TBI 2006-2010 with continuous drug coverage for 12 months before and after TBI (n=60,276). Measurements—We obtained monthly psychotropic medication use by drug class and specific drugs from Medicare Part D drug event files. International Classification of Disease, 9th Edition CM, codes were used to define anxiety (300.0x) and PTSD (309.81). Results—Average monthly …

Glucagon And Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription By Increasing The Expression Of Activating Transcription Factor 4, Kimberly M. Alonge, Gordon P. Meares, F. Bradley Hillgartner

Clinical and Translational Science Institute

Previous studies have shown that glucagon cooperatively interacts with insulin to stimulate hepatic FGF21 gene expression. Here we investigated the mechanism by which glucagon and insulin increased FGF21 gene transcription in primary hepatocyte cultures. Transfection analyses demonstrated that glucagon plus insulin induction of FGF21 transcription was conferred by two activating transcription factor 4 (ATF4) binding sites in the FGF21 gene. Glucagon plus insulin stimulated a 5-fold increase in ATF4 protein abundance, and knockdown of ATF4 expression suppressed the ability of glucagon plus insulin to increase FGF21 expression. In hepatocytes incubated in the presence of insulin, treatment with a PKA-selective agonist …

Quick Guide Pasture And Grazing Management.Pdf, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Into The Wild: Parallel Transcriptomics Of The Tsetse-Wigglesworthia Mutualism Within Kenyan Populations, Miguel M. Munoz, Adam R. Pollio, Hunter L. White, Rita V M Rio

Faculty & Staff Scholarship

Tsetse flies (Diptera: Glossinidae) have medical significance as the obligate vectors of African trypanosomes. In addition, tsetse harbor a simple gut microbiota. A predominant gut microbiota member, the Gammaproteobacterium Wigglesworthia spp., has coevolved with tsetse for a significant portion of Glossina radiation proving critical to tsetse fitness. Although multiple roles have been described for Wigglesworthia within colony flies, little research has been dedicated towards functional characterization within wild tsetse. Here, dual RNA-Seq was performed to characterize the tsetse-Wigglesworthia symbiosis within flies captured in Nguruman, Kenya. A significant correlation in Gene Ontology (GO) distribution between tsetse and Wigglesworthia was observed, with …

2017 Symposium Brochure

Programs

No abstract provided.

Fibrous Nanocellulose, Crystalline Nanocellulose, Carbon Nanotubes, And Crocidolite Asbestos Elicit Disparate Immune Responses Upon Pharyngeal Aspiration In Mice, Eun-Jung Park, Timur O. Khaliullin, Michael R. Shurin, Elena R. Kisin, Naveena Yanamala, Bengt Fadeel, Jaerak Chang, Anna A. Shvedova

Faculty & Staff Scholarship

With the rapid development of synthetic alternatives to mineral fibers, their possible effects on the environment and human health have become recognized as important issues worldwide. This study investigated effects of four fibrous materials, i.e. nanofibrillar/nanocrystalline celluloses (NCF and CNC), single-walled carbon nanotubes (CNTs), and crocidolite asbestos (ASB), on pulmonary inflammation and immune responses found in the lungs, as well as the effects on spleen and peripheral blood immune cell subsets. BALB/c mice were given NCF, CNC, CNT, and ASB on Day 1 by oropharyngeal aspiration. At 14 days post-exposure, the animals were evaluated. Total cell number, mononuclear phagocytes, polymorphonuclear …

Volatility Spillover And Time-Varying Conditional Correlation Between Ddgs, Corn, And Soybean Meal Markets, Xiaoli L. Etienne, Andres Trujillo-Barrera, Linwood A. Hoffman

Faculty & Staff Scholarship

We find distiller's dried grains with solubles (DDGS) prices to be positively correlated with both corn and soybean meal prices in the long run. However, neither corn nor soybean meal prices respond to deviations from this long-run relationship. We also identify strong time-varying dynamic conditional correlations between the markets, with the correlation between DDGS and corn strengthened after the expansion of ethanol production. There also appear to exist significant volatility spillovers from both the corn and soybean meal markets to the DDGS market, with the impact from corn shocks much larger compared to soybean meal shocks.

Healthcare Resource Availability And Cardiovascular Health In The Usa, Courtney S. Pilkerton, Sarah S. Singh, Thomas K. Bias, Stephanie J. Frisbee

Faculty & Staff Scholarship

Objectives Cardiovascular disease (CVD) remains the leading cause of death in the USA. Reducing the population-level burden of CVD disease will require a better understanding and support of cardiovascular health (CVH) in individuals and entire communities. The objectives for this study were to examine associations between community-level healthcare resources (HCrRes) and CVH in individuals and entire communities.

Setting This study consisted of a retrospective, cross-sectional study design, using multivariable epidemiological analyses.

Participants All participants in the 2011 Behavioral Risk Factor Surveillance System (BRFSS) survey were examined for eligibility. CVH, defined using the American Heart Association CVH Index (CVHI), was determined …

Pancreatic Divisum: An Unusual Cause Of Chronic Pancreatitis In A Young Patient, Aaron R. Kuzel, Muhammad U. Lodhi, Mustafa Rahim

Faculty & Staff Scholarship

Pancreatic divisum is a condition that occurs in 4-14% of the population. Pancreatic divisum occurs in development when the ventral bud and dorsal bud of the pancreas fail to fuse. Patients with this condition are usually asymptomatic, however, 25-38% of these patients experience recurrent pancreatitis that may further progress to chronic pancreatitis. This case is of a 20-year-old female presenting with abdominal pain in the left and right upper quadrants of the abdomen with a significant history of recurrent pancreatitis since the age of seven. The patient was examined with computed tomography (CT), which identified pancreatitis. Further magnetic resonance cholangiopancreatography …

Promoting Professional Identity, Motivation, And Persistence: Benefits Of An Informal Mentoring Program For Female Undergraduate Students, Paul R. Hernandez, Brittany Bloodhart, Rebecca T. Barnes, Amanda S. Adams, Sandra M. Clinton, Ilana Pollack, Elaine Godfrey, Melissa Burt, Emily V. Fisher

Faculty & Staff Scholarship

Women are underrepresented in a number of science, technology, engineering, and mathe- matics (STEM) disciplines. Limited diversity in the development of the STEM workforce has negative implications for scientific innovation, creativity, and social relevance. The current study reports the first-year results of the PROmoting Geoscience Research, Education, and SuccesS (PROGRESS) program, a novel theory-driven informal mentoring program aimed at supporting first- and second-year female STEM majors. Using a prospective, longitudinal, multi-site (i.e., 7 universities in Colorado/Wyoming Front Range & Carolinas), propensity score matched design, we compare mentoring and persistence outcomes for women in and out of PROGRESS (N = 116). …

Functional Role Of Cyclin-Dependent Kinase 5 In The Regulation Of Melanogenesis And Epidermal Structure, Changsheng Dong, Shanshan Yang, Ruiwen Fan, Kaiyuan Ji, Junzhen Zhang, Xuexian Liu, Shuaipeng Hu, Jianshan Xie, Yu Liu, Wenjun Gao, Haidong Wang, Jianbo Yao, George W. Smith, Muren Herrid

Faculty & Staff Scholarship

The mammalian integumentary system plays important roles in body homeostasis, and dysfunction of melanogenesis or epidermal development may lead to a variety of skin diseases, including melanoma. Skin pigmentation in humans and coat color in fleece-producing animals are regulated by many genes. Among them, microphthalmia-associated transcription factor (MITF) and paired-box 3 (PAX3) are at the top of the cascade and regulate activities of many important melanogenic enzymes. Here, we report for the first time that cyclin-dependent kinase 5 (Cdk5) is an essential regulator of MITF and PAX3. Cdk5 knockdown in mice causes a lightened coat …

Health-Promoting Phytochemicals From 11 Mustard Cultivars At Baby Leaf And Mature Stages, Marissa D. Frazie, Moo J. Kim, Kang-Mo Ku

Faculty & Staff Scholarship

Mustard is a Brassica vegetable that provides a number of phytonutrients. However, the phytonutrient profile of mustard has been relatively limited. We analyzed the glucosinolates and their hydrolysis products, carotenoids, total anthocyanin and phenolic contents, and antioxidant capacity of the leaves of 11 mustard cultivars grown in a greenhouse at the baby leaf and mature stages. An aliphatic glucosinolate sinigrin and its hydrolysis products allyl isothiocyanate and 1-cyano-2,3-epithiopropane were the major phytonutrients in the mustard leaves. Carotenoids β-carotene, lutein, violaxanthin, and neoxanthin were detected. We found phytonutrient concentration and their change with plant growth were cultivar-dependent. The %RDA value for …

Chemopreventive Glucosinolate Accumulation In Various Broccoli And Collard Tissues: Microfluidic-Based Targeted Transcriptomics For By-Product Valorization, Young-Sang Lee, Kang-Mo Ku, John A. Juvik

Faculty & Staff Scholarship

Floret, leaf, and root tissues were harvested from broccoli and collard cultivars and extracted to determine their glucosinolate and hydrolysis product profiles using high performance liquid chromatography and gas chromotography. Quinone reductase inducing bioactivity, an estimate of anti-cancer chemopreventive potential, of the extracts was measured using a hepa1c1c7 murine cell line. Extracts from root tissues were significantly different from other tissues and contained high levels of gluconasturtiin and glucoerucin. Targeted gene expression analysis on glucosinolate biosynthesis revealed that broccoli root tissue has elevated gene expression of AOP2 and low expression of FMOGS-OX homologs, essentially the opposite of what was observed …

Comprehensive Analysis Of Lncrnas And Mrnas In Skeletal Muscle Of Rainbow Trout (Oncorhynchus Mykiss) Exposed To Estradiol, Jian Wang, Pransanthi P. Kogan, Jianbo Yao, Shuo Wei, Beth Cleveland

Faculty & Staff Scholarship

Estradiol (E2) is a steroid hormone that negatively affects muscle growth in rainbow trout (Oncorhynchus mykiss), but the mechanisms directing with this response are not fully understood. To better characterize the effects of E2 in muscle, we identified differentially regulated mRNAs and lncRNAs in juvenile rainbow trout exposed to E2. Here, we performed next-generation RNA sequencing and comprehensive bioinformatics analyses to characterize the transcriptome profiles, including mRNAs and long noncoding RNAs (lncRNAs), in skeletal muscle of rainbow trout injected with E2. A total of 226 lncRNAs and 253 mRNAs were identified as differentially regulated. We identified crucial pathways, including several …

Two Types Of Bone Necrosis In The Middle Triassic Pistosaurus Longaevus Bones: The Results Of Integrated Studies, Dawid Surmik, Bruce M. Rothschild, Mateusz Dulski, Katarzyna Janiszewska

Faculty & Staff Scholarship

Avascular necrosis, diagnosed on the basis of either a specific pathological modification of the articular surfaces of bone or its radiologic appearance in vertebral centra, has been recognized in many Mesozoic marine reptiles as well as in present-day marine mammals. Its presence in the zoological and paleontologic record is usually associated with decompression syndrome, a disease that affects secondarily aquatic vertebrates that could dive. Bone necrosis can also be caused by infectious processes, but it differs in appearance from decompression syndrome-associated aseptic necrosis. Herein, we report evidence of septic necrosis in the proximal articular surface of the femur of a …

Impact Of Built-Up-Litter And Commercial Antimicrobials On Salmonella And Campylobacter Contamination Of Broiler Carcasses Processed At A Pilot Mobile Poultry-Processing Unit, Kawang Li, Lacey Lemonakis, Brian Glover, Joseph Moritz, Cangliang Shen

Faculty & Staff Scholarship

The small-scale mobile poultry-processing unit (MPPU) produced raw poultry products are of particular food safety concern due to exemption of USDA poultry products inspection act. Limited studies reported the microbial quality and safety of MPPU-processed poultry carcasses. This study evaluated the Salmonella and Campylobacter prevalence in broiler ceca and on MPPU-processed carcasses and efficacy of commercial antimicrobials against Campylobacter jejuni on broilers. In study I, straight-run Hubbard × Cobb broilers (147) were reared for 38 days on clean-shavings (CS, 75) or built-up-litter (BUL, 72) and processed at an MPPU. Aerobic plate counts (APCs), coliforms, Escherichia coli, and yeast/molds (Y/M) …

Expression Of Cocaine- And Amphetamine-Regulated Transcript (Cart) In Hen Ovary, Pengfei Li, Jianshan Xie, Xiaolei Yao, Wenzhong Liu, Jianbo Yao, Zhiwei Zhu, Lihua Lyu

Faculty & Staff Scholarship

Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and …

Cultivar-Specific Changes In Primary And Secondary Metabolites In Pak Choi (Brassica Rapa, Chinensis Group) By Methyl Jasmonate, Moo J. Kim, Yu-Chun Chiu, Na K. Kim, Hye M. Park, Choong H. Lee, John A. Juvik, Kang-Mo Ku

Faculty & Staff Scholarship

Glucosinolates, their hydrolysis products and primary metabolites were analyzed in five pak choi cultivars to determine the effect of methyl jasmonate (MeJA) on metabolite flux from primary metabolites to glucosinolates and their hydrolysis products. Among detected glucosinolates (total 14 glucosinolates; 9 aliphatic, 4 indole and 1 aromatic glucosinolates), indole glucosinolate concentrations (153–229%) and their hydrolysis products increased with MeJA treatment. Changes in the total isothiocyanates by MeJA were associated with epithiospecifier protein activity estimated as nitrile formation. Goitrin, a goitrogenic compound, significantly decreased by MeJA treatment in all cultivars. Changes in glucosinolates, especially aliphatic, significantly differed among cultivars. Primary metabolites …

Patient Characteristics And Perceived Health Status Of Individuals With Hiv And Tuberculosis Coinfection In Guangxi, China, Yujia Zhu, Jizhou Wu, Xue Feng, Huanhuan Chen, Huaxiang Lu, Luo Li, Chao Rui

Faculty & Staff Scholarship

To explore demographics, clinical and medication profiles, patients’ social support, and perceived health status in HIV/TB coinfected patients in Guangxi, China.

We performed a cross-sectional study in the HIV clinic of the Guigang City People's Hospital (N = 150). Health professionals conducted face-to-face interviews and collected data from patients’ electronic medical records regarding patients’ demographic, clinical, and medication information, as well as their social support and perceived health status. We classified all HIV/AIDS patients into HIV monoinfected and TB coinfected, at a ratio of 2:1.

Compared with the HIV monoinfected, patients with HIV/TB coinfection were more likely to be older, …

Glucosinolates, Carotenoids, And Vitamins E And K Variation From Selected Kale And Collard Cultivars, Moo J. Kim, Yu-Chun Chiu, Kang-Mo Ku

Faculty & Staff Scholarship

Glucosinolates, carotenoids, and fat-soluble vitamins E and K contents were analyzed from various kale and collard cultivars at mature stage. We found a significant difference in these phytonutrients among cultivars. Among kale cultivars, “Beira” and “Olympic Red” were the highest in the total glucosinolate and “Toscano” kale was the highest in total carotenoid content. “Scarlet” kale was highest in tocopherols. For collard, total glucosinolate was the highest in “Top Bunch” while carotenoids were the highest in “Green Glaze.” An accession PI261597 was the highest in phylloquinone. In addition to the total content of each phytonutrient class, their composition differed among …

The Effect Of Adherence To Dietary Tracking On Weight Loss: Using Hlm To Model Weight Loss Over Time, John S. Ingles, Ranjita Misra, Jonathan Stewart, Brandon Luke-Wold, Samantha Shawley-Brzoska

Faculty & Staff Scholarship

The role of dietary tracking on weight loss remains unexplored despite being part of multiple diabetes and weight management programs. Hence, participants of the Diabetes Prevention and Management (DPM) program (12 months, 22 sessions) tracked their food intake for the duration of the study. A scatterplot of days tracked versus total weight loss revealed a nonlinear relationship. Hence, the number of possible tracking days was divided to create the 3 groups of participants: rare trackers (66% total days tracked). After controlling for initial body mass index, hemoglobin A1c, and gender, only consistent trackers had significant weight loss (−9.99 pounds), following …

Winter Grazing In A Grass-Fed System: Effect Of Stocking Density And Sequential Use Of Autumn-Stockpiled Grassland On Performance Of Yearling Steers, Domingo J. Mata-Padrino, E E D Felton, W B. Bryan, D P. Belesky

Faculty & Staff Scholarship

Winter grazing can help reduce the need for purchased feeds in livestock production systems, when finishing cattle on pasture. Our objective was to evaluate the influence of stocking density and grazing stockpiled forage on performance of yearling steers during winter. Three grasslands were winter grazed for two years: I, naturalized pastureland, and II and III, sown and managed for hay production during the growing season but grazed in winter. Two stocking densities were used: low 7.41 and high 12.35 steers ha−1. Herbage mass was estimated before and after each grazing event, and disappearance (consumption, weathering, and trampling) was the difference …

Agonist Muscle Adaptation Accompanied By Antagonist Muscle Atrophy In The Hindlimb Of Mice Following Stretch-Shortening Contraction Training, Erik P. Rader, Marshall A. Naimo, James Ensey, Brent A. Baker

Faculty & Staff Scholarship

Background: The vast majority of dynamometer-based animal models for investigation of the response to chronic muscle contraction exposure has been limited to analysis of isometric, lengthening, or shortening contractions in isolation. An exception to this has been the utilization of a rat model to study stretch-shortening contractions (SSCs), a sequence of consecutive isometric, lengthening, and shortening contractions common during daily activity and resistance-type exercise. However, the availability of diverse genetic strains of rats is limited. Therefore, the purpose of the present study was to develop a dynamometer-based SSC training protocol to induce increased muscle mass and performance in plantarflexor muscles …

Lhx8 Interacts With A Novel Germ Cell-Specific Nuclear Factor Containing An Nbl1 Domain In Rainbow Trout (Oncorhynchus Mykiss), Liyuan Fu, Prasanthi P. Koganti, Jian Wang, Lei Wang, Cheng-Lun Wang, Jianbo Yao

Faculty & Staff Scholarship

Lhx8 is an important transcription factor that is preferentially expressed in germ cells. Lhx8 null mice are infertile due to lack of oocytes and impairment of the transition from primordial follicles to primary follicles. Lhx8 deficiency also affects the expression of many important oocyte-specific genes. In this study, we report the characterization of rainbow trout lhx8 genes and identification of a novel germ cell-specific nuclear factor that interacts with Lhx8. Two lhx8genes, lhx8a and lhx8b, were identified, encoding proteins of 344 and 361 amino acids, respectively. The two proteins share 83% sequence identity and both transcripts are specifically expressed in …

Recruiting Women To A Mobile Health Smoking Cessation Trial: Low- And No-Cost Strategies, Kristopher J. Abbate, Melanie D. Hingle, Julie Armin, Peter Giacobbi, Judith S. Gordon

Faculty & Staff Scholarship

Background: Successful recruitment of participants to mobile health (mHealth) studies presents unique challenges over in-person studies. It is important to identify recruitment strategies that maximize the limited recruitment resources available to researchers.

Objective: The objective of this study was to describe a case study of a unique recruitment process used in a recent mHealth software app designed to increase smoking cessation among weight-concerned women smokers. The See Me Smoke-Free app was deployed to the Google Play Store (Alphabet, Inc., Google, LLC), where potential participants could download the app and enroll in the study. Users were invited in-app to participate in …