Digital Commons Network^™

Chemopreventive Glucosinolate Accumulation In Various Broccoli And Collard Tissues: Microfluidic-Based Targeted Transcriptomics For By-Product Valorization, Young-Sang Lee, Kang-Mo Ku, John A. Juvik

Faculty & Staff Scholarship

Floret, leaf, and root tissues were harvested from broccoli and collard cultivars and extracted to determine their glucosinolate and hydrolysis product profiles using high performance liquid chromatography and gas chromotography. Quinone reductase inducing bioactivity, an estimate of anti-cancer chemopreventive potential, of the extracts was measured using a hepa1c1c7 murine cell line. Extracts from root tissues were significantly different from other tissues and contained high levels of gluconasturtiin and glucoerucin. Targeted gene expression analysis on glucosinolate biosynthesis revealed that broccoli root tissue has elevated gene expression of AOP2 and low expression of FMOGS-OX homologs, essentially the opposite of what was observed …

In Vitro Progesterone Production By Luteinized Human Mural Granulosa Cells Is Modulated By Activation Of Ampk And Cause Of Infertility, E C Bowdridge, M W Vernon, J A Flores, M J Clemmer

Faculty & Staff Scholarship

Background: Mural granulosa cells from IVF patients were provided by the West Virginia University Center for Reproductive Medicine in Morgantown, WV. The effect of adenosine monophosphate activated protein kinase (AMPK) activation, primary cause of infertility, age, BMI, and pregnancy outcome on production of progesterone were examined separately.

Methods: Isolated mural sheets from IVF patients (n = 26) were centrifuged, supernatant discarded, and the pellet re-suspended in 500 μl of DMEM/F12. Mural granulosa cells were plated at 10,000 cells/well in triplicate per treatment group with 300 μl DMEM/F12 media at 37 °C and 5% CO2 in a humidified incubator to permit …

The Oldest Record Of Aquatic Amniote Congenital Scoliosis, Tomasz Szczygielski, Dawid Surmik, Agnieszka Kapuscinska, Bruce M. Rotheschild

Faculty & Staff Scholarship

We report the first occurrence of congenital scoliosis in an early Permian aquatic parareptile, Stereosternum tumidum from Parana ́ state, Brazil. The spine malformation is caused by a congenital hemivertebra. These observations give insight into the biomechanical aspects of underwater locomotion in an axial skeleton-compromised aquatic amniote. This is the oldest record of a hemivertebra in an aquatic animal.

Comprehensive Analysis Of Lncrnas And Mrnas In Skeletal Muscle Of Rainbow Trout (Oncorhynchus Mykiss) Exposed To Estradiol, Jian Wang, Pransanthi P. Kogan, Jianbo Yao, Shuo Wei, Beth Cleveland

Faculty & Staff Scholarship

Estradiol (E2) is a steroid hormone that negatively affects muscle growth in rainbow trout (Oncorhynchus mykiss), but the mechanisms directing with this response are not fully understood. To better characterize the effects of E2 in muscle, we identified differentially regulated mRNAs and lncRNAs in juvenile rainbow trout exposed to E2. Here, we performed next-generation RNA sequencing and comprehensive bioinformatics analyses to characterize the transcriptome profiles, including mRNAs and long noncoding RNAs (lncRNAs), in skeletal muscle of rainbow trout injected with E2. A total of 226 lncRNAs and 253 mRNAs were identified as differentially regulated. We identified crucial pathways, including several …

Sox9 Regulates Cancer Stem-Like Properties And Metastatic Potential Of Single-Walled Carbon Nanotube-Exposed Cells, Maria A. Voronkova, Sudjit Luanpitpong, Liying W. Rojanasakul, Vincent Castranova, Cerasela Z. Dinu, Heimo Riedel, Yon Rojanasakul

Faculty & Staff Scholarship

Engineered nanomaterials hold great promise for the future development of innovative products but their adverse health effects are a major concern. Recent studies have indicated that certain nanomaterials, including carbon nanotubes (CNTs), may be carcinogenic. However, the underlying mechanisms behind their potential malignant properties remain unclear. In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotubes (SWCNTs). We found

that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. …

Cell-Specific Gain Modulation By Synaptically Released Zinc In Cortical Circuits Of Audition, Charles T. Anderson, Manoj Kumar, Shanshan Xiong, Thanos Tzounopolos

Faculty & Staff Scholarship

In many excitatory synapses, mobile zinc is found within glutamatergic vesicles and is coreleased with glutamate. Ex vivo studies established that synaptically released (synaptic) zinc inhibits excitatory neurotransmission at lower frequencies of synaptic activity but enhances steady state synaptic responses during higher frequencies of activity. However, it remains unknown how synaptic zinc affects neuronal processing in vivo. Here, we imaged the sound-evoked neuronal activity of the primary auditory cortex in awake mice. We discovered that synaptic zinc enhanced the gain of sound-evoked responses in CaMKII-expressing principal neurons, but it reduced the gain of parvalbumin- and somatostatin-expressing interneurons. This modulation was …

Wholly Rickettsia! Reconstructed Metabolic Profile Of The Quintessential Bacterial Parasite Of Eukaryotic Cells, Timothy P. Driscoll, Victoria I. Verhoeve, Mark L. Guillotte, Stephanie S. Lehman, Sherri A. Rennoll, Magda Beier-Sexton, M Sayeedur Rahman, Abdu F. Azad, Joseph J. Gillespie

Faculty & Staff Scholarship

Reductive genome evolution has purged many metabolic pathways from obligate intracellular Rickettsia (Alphaproteobacteria; Rickettsiaceae). While some aspects of host-dependent rickettsial metabolism have been characterized, the array of host-acquired metabolites and their cognate transporters remains unknown. This dearth of information has thwarted efforts to obtain an axenic Rickettsia culture, a major impediment to conventional genetic approaches. Using phylogenomics and computational pathway analysis, we reconstructed the Rickettsia metabolic and transport network, identifying 51 host-acquired metabolites (only 21 previously characterized) needed to compensate for degraded biosynthesis pathways. In the absence of glycolysis and the pentose phosphate pathway, cell envelope glycocon- jugates are synthesized …

Enriching Traditional Protein-Protein Interaction Networks With Alternative Conformations Of Proteins, Farideh Halakou, Emel S. Kilic, Engin Cukuroglu, Ozlem Keskin, Attila Gursoy

Faculty & Staff Scholarship

Traditional Protein-Protein Interaction (PPI) networks, which use a node and edge representation, lack some valuable information about the mechanistic details of biological processes. Mapping protein structures to these PPI networks not only provides structural details of each interaction but also helps us to find the mutual exclusive interactions. Yet it is not a comprehensive representation as it neglects the conformational changes of proteins which may lead to different interactions, functions, and downstream signalling. In this study, we proposed a new representation for structural PPI networks inspecting the alternative conformations of proteins. We performed a large-scale study by creating breast cancer …

Two Types Of Bone Necrosis In The Middle Triassic Pistosaurus Longaevus Bones: The Results Of Integrated Studies, Dawid Surmik, Bruce M. Rothschild, Mateusz Dulski, Katarzyna Janiszewska

Faculty & Staff Scholarship

Avascular necrosis, diagnosed on the basis of either a specific pathological modification of the articular surfaces of bone or its radiologic appearance in vertebral centra, has been recognized in many Mesozoic marine reptiles as well as in present-day marine mammals. Its presence in the zoological and paleontologic record is usually associated with decompression syndrome, a disease that affects secondarily aquatic vertebrates that could dive. Bone necrosis can also be caused by infectious processes, but it differs in appearance from decompression syndrome-associated aseptic necrosis. Herein, we report evidence of septic necrosis in the proximal articular surface of the femur of a …

Impact Of Built-Up-Litter And Commercial Antimicrobials On Salmonella And Campylobacter Contamination Of Broiler Carcasses Processed At A Pilot Mobile Poultry-Processing Unit, Kawang Li, Lacey Lemonakis, Brian Glover, Joseph Moritz, Cangliang Shen

Faculty & Staff Scholarship

The small-scale mobile poultry-processing unit (MPPU) produced raw poultry products are of particular food safety concern due to exemption of USDA poultry products inspection act. Limited studies reported the microbial quality and safety of MPPU-processed poultry carcasses. This study evaluated the Salmonella and Campylobacter prevalence in broiler ceca and on MPPU-processed carcasses and efficacy of commercial antimicrobials against Campylobacter jejuni on broilers. In study I, straight-run Hubbard × Cobb broilers (147) were reared for 38 days on clean-shavings (CS, 75) or built-up-litter (BUL, 72) and processed at an MPPU. Aerobic plate counts (APCs), coliforms, Escherichia coli, and yeast/molds (Y/M) …

Mti-101 Treatment Inducing Activation Of Stim1 And Trpc1 Expression Is A Determinant Of Response In Multiple Myeloma, Michael F. Emmons, Nagaraju Anreddy, Kayla Steinberger, Shengyu Yang, Mark Mclaughlin, Ariosto Silva, Lori A. Hazlehurst

Faculty & Staff Scholarship

The emergence of drug resistance continues to be a major hurdle towards improving patient outcomes for the treatment of Multiple Myeloma. MTI-101 is a first-in-class peptidomimetic that binds a CD44/ITGA4 containing complex and triggers necrotic cell death in multiple myeloma cell lines. In this report, we show that acquisition of resistance to MTI-101 correlates with changes in expression of genes predicted to attenuate Ca2+ flux. Consistent with the acquired resistant genotype, MTI-101 treatment induces a rapid and robust increase in intracellular Ca2+ levels in the parental cells; a finding that was attenuated in the acquired drug resistant cell line. Mechanistically, …

Long Non-Coding Rna Atb Promotes Malignancy Of Esophageal Squamous Cell Carcinoma By Regulating Mir-200b/Kindlin-2 Axis, Zhongwen Li, Xiaoliang Wu, Qi Shen, Wen Luo, Chuangzhong Deng, Qianghua Zhou, Xinru Chen, Yanjie Li, Zuanfu Lim, Xing Wang, Jiahong Wang, Xianzi Yang

Faculty & Staff Scholarship

Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer-related death, especially in China. In addition, the prognosis of late stage patients is extremely poor. However, the biological significance of the long non-coding RNA lnc-ATB and its potential role in ESCC remain to be documented. In this study, we investigated the role of lnc-ATB and the underlying mechanism promoting its oncogenic activity in ESCC. Expression of lnc-ATB was higher in ESCC tissues and cell lines than that in normal counterparts. Upregulated lnc-ATB served as an independent prognosis predictor of ESCC patients. Moreover, loss-of-function assays in ESCC cells …

Expression Of Cocaine- And Amphetamine-Regulated Transcript (Cart) In Hen Ovary, Pengfei Li, Jianshan Xie, Xiaolei Yao, Wenzhong Liu, Jianbo Yao, Zhiwei Zhu, Lihua Lyu

Faculty & Staff Scholarship

Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and …

Heterogeneous Vascular Bed Responses To Pulmonary Titanium Dioxide Nanoparticle Exposure, Alaeddin B. Abukabda, Phoebe A. Stapleton, Carroll R. Mcbride, Jinghai Yi, Timothy R. Nurkiewicz

Faculty & Staff Scholarship

A growing body of research links engineered nanomaterial (ENM) exposure to adverse cardiovascular endpoints. The purpose of this study was to evaluate the impact of ENM exposure on vascular reactivity in discrete segments so that we may determine the most sensitive levels of the vasculature where these negative cardiovascular effects are manifest. We hypothesized that acute nano-TiO2exposure differentially affects reactivity with a more robust impairment in the microcirculation. Sprague-Dawley rats (8–10 weeks) were exposed to nano-TiO2via intratracheal instillation (20, 100, or 200 µg suspended per 250 µL of vehicle) 24 h prior to vascular assessments. A serial assessment across …

Development And Design Of Next-Generation Head-Mounted Ambulatory Microdose Positron-Emission Tomography (Am-Pet) System, Samantha Melroy, Christopher Bauer, Matthew Mchugh, Garret Carden, Alexander Stolin, Stan Majewski, Julie Bredczynski-Lewis, Thorsten Wuest

Faculty & Staff Scholarship

Several applications exist for a whole brain positron-emission tomography (PET) brain imager designed as a portable unit that can be worn on a patient’s head. Enabled by improvements in detector technology, a lightweight, high performance device would allow PET brain imaging in different environments and during behavioral tasks. Such a wearable system that allows the subjects to move their heads and walk—the Ambulatory Microdose PET (AM-PET)—is currently under development. This imager will be helpful for testing subjects performing selected activities such as gestures, virtual reality activities and walking. The need for this type of lightweight mobile device has led to …

Inhibition Of Vegf And Angiopoietin-2 To Reduce Brain Metastases Of Breast Cancer Burden, Kaci A. Bohn, Chris E. Adkins, Mohamed I. Nounou, Paul R. Lockman

Faculty & Staff Scholarship

For metastases in the central nervous system, angiogenesis enhances metastatic potential and promotes progression. Primary factors which drive vessel growth are vascular endothelial growth factor (VEGF) and angiopoietin-2. Preclinical models show inhibition of either factor reduces metastases spread and inhibits growth. This work sets out to answer two questions in a preclinical mouse model. First, whether the combined inhibition of VEGF and angiopoietin-2, reduces passive permeability and limits drug uptake into brain metastases; and second, whether this inhibition reduces metastases burden in brain. We observed combinatorial inhibition of VEGF and angiopoietin-2, decreased (p < 0.05) angiogenesis and vascular branching in an aortic ring assay and decreased (p < 0.05) endothelial wound closure times. Using a brain metastases of breast cancer model (induced by intracardiac injections of brain seeking MDA-MB-231Br cells or 4T1Br cells), we observed, similar to VEGF, angiopoetin-2 expression correlates to increased angiogenesis (p < 0.05) and increased lesion permeability. To determine efficacy, animals were administered bevacizumab plus L1-10 (angiopoietin inhibitor) twice per week until neurological symptoms developed. Lesion permeability significantly decreased by ∼50% (p < 0.05) compared to untreated lesions, but remained ∼25% greater (p < 0.0%) than brain. In subsequent experiments, animals were administered similar regimens but sacrificed on day 32. The number of metastatic lesions developed was significantly (p < 0.001) reduced in the bevacizumab group (56%) and combination group (86%). Lesions’ size was reduced in bevacizumab treated lesions (∼67%) and bevacizumab and L1-10 treated lesions (∼78%) developing area < 0.5 mm2. In summary, combinatorial inhibition of VEGF and angiopoietin reduces lesion permeability and brain metastatic burden.

Male Fertility Status Is Associated With Dna Methylation Signatures In Sperm And Transcriptomic Profiles Of Bovine Preimplantation Embryos, Jenna Kropp, Jose A. Carrillo, Hadjer Namous, Alyssa Daniels, Sana M. Salih, Jiuzhou Song, Hasan Khatib

Faculty & Staff Scholarship

Background

Infertility in dairy cattle is a concern where reduced fertilization rates and high embryonic loss are contributing factors. Studies of the paternal contribution to reproductive performance are limited. However, recent discoveries have shown that, in addition to DNA, sperm delivers transcription factors and epigenetic components that are required for fertilization and proper embryonic development. Hence, characterization of the paternal contribution at the time of fertilization is warranted. We hypothesized that sire fertility is associated with differences in DNA methylation patterns in sperm and that the embryonic transcriptomic profiles are influenced by the fertility status of the bull. Embryos were …

Vascular Endothelial Overexpression Of Human Cyp2j2 (Tie2-Cyp2j2 Tr) Modulates Cardiac Oxylipin Profiles And Enhances Coronary Reactive Hyperemia In Mice, Ahmad Hanif, Matthew L. Edin, Darryl C. Zeldin, Chridtophe Morisseau, John R. Falck, Mohammed A. Nayeem

Faculty & Staff Scholarship

Arachidonic acid is metabolized to epoxyeicosatrienoic acids (EETs) by cytochrome (CYP) P450 epoxygenases, and to ω-terminal hydroxyeicosatetraenoic acids (HETEs) by ω-hydroxylases. EETs and HETEs often have opposite biologic effects; EETs are vasodilatory and protect against ischemia/reperfusion injury, while ω-terminal HETEs are vasoconstrictive and cause vascular dysfunction. Other oxylipins, such as epoxyoctadecaenoic acids (EpOMEs), hydroxyoctadecadienoic acids (HODEs), and prostanoids also have varied vascular effects. Post-ischemic vasodilation in the heart, known as coronary reactive hyperemia (CRH), protects against potential damage to the heart muscle caused by ischemia. The relationship among CRH response to ischemia, in mice with altered levels of CYP2J epoxygenases …

Mesothelin Promotes Epithelial-To-Mesenchymal Transition And Tumorigenicity Of Human Lung Cancer And Mesothelioma Cells, Xiaoqing He, Liying Wang, Kai Wang, Yong Yang, Cerasela Z. Dinu, Yon Ronjanasakul

Faculty & Staff Scholarship

Background

Lung cancer and pleural mesothelioma are two of the most deadly forms of cancer. The prognosis of lung cancer and mesothelioma is extremely poor due to limited treatment modalities and lack of understanding of the disease mechanisms. We have identified mesothelin as a potentially unique therapeutic target that as a specific advantage appears nonessential in most cell types. Mesothelin (MSLN), a plasma membrane differentiation antigen, is expressed at a high level in many human solid tumors, including 70% of lung cancer and nearly all mesotheliomas. However, the role of MSLN in the disease process and underlying mechanisms is largely …

Altering Calcium Influx For Selective Destruction Of Breast Tumor, Han-Gang Yu, Sarah Mclaughlin, Mackenzie Newman, Kathleen Brundage, Amanda Ammer, Karen Martin, James Coad

Faculty & Staff Scholarship

Background

Human triple-negative breast cancer has limited therapeutic choices. Breast tumor cells have depolarized plasma membrane potential. Using this unique electrical property, we aim to develop an effective selective killing of triple-negative breast cancer.

Methods

We used an engineered L-type voltage-gated calcium channel (Cec), activated by membrane depolarization without inactivation, to induce excessive calcium influx in breast tumor cells. Patch clamp and flow cytometry were used in testing the killing selectivity and efficiency of human breast tumor cells in vitro. Bioluminescence and ultrasound imaging were used in studies of human triple-negative breast cancer cell MDA-MB-231 xenograft in mice. Histological staining, …

Nicotinic Acid Inhibits Glioma Invasion By Facilitating Snail1 Degradation, Jiejing Li, Jiagui Qu, Yu Shi, Mark Perfetto, Zhuxian Ping, Laura Christian, Hua Niu, Shuting Mei, Qin Zhang, Xiangcai Yang, Shuo Wei

Faculty & Staff Scholarship

Malignant glioma is a formidable disease that commonly leads to death, mainly due to the invasion of tumor cells into neighboring tissues. Therefore, inhibition of tumor cell invasion may provide an effective therapy for malignant glioma. Here we report that nicotinic acid (NA), an essential vitamin, inhibits glioma cell invasion in vitro and in vivo. Treatment of the U251 glioma cells with NA in vitroresults in reduced invasion, which is accompanied by a loss of mesenchymal phenotype and an increase in cell-cell adhesion. At the molecular level, transcription of the adherens junction protein E-cadherin is upregulated, leading to …

Influence Of 1-Methylcyclopropene Treatment On Postharvest Quality Of Four Scab (Venturia Inaequalis)-Resistant Apple Cultivars, Moises Zucoloto, Kang-Mo Ku, Moo J. Kim, Mosbah M. Kushad

Faculty & Staff Scholarship

Scab (Venturia inaequalis) is a very serious disease for apples causing up to 80% of loss in yield but there are only a few studies on postharvest quality of scab-resistant cultivars. In this study we evaluated the effect of 1-methylcyclopropene (1-MCP) on fruit quality, total phenolic content, and antioxidant capacity after storage of four scab-resistant cultivars and compared to a standard cultivar, “Golden Delicious.” In general, ethylene production and respiration rates significantly differed among cultivars, between control and 1-MCP-treated fruits, and between storage duration regimes. 1-MCP treatment retarded fruit softening and lowered juice pH but storage effect on soluble solids …

Nonvitamin, Nonmineral Dietary Supplement Use Among Adults With Fibromyalgia: United States, 2007–2012, Termeh Feinberg, Christa Lilly, Kim Innes

Faculty & Staff Scholarship

Background. Fibromyalgia (FMS) is a pain condition affecting 2–6% of US adults; effective treatment remains limited. Determinants of nonvitamin, nonmineral dietary supplement (NVNM) use among adults with FMS are not well-studied. We investigated the relation of NVNM use to FMS, and trends, in two nationally representative samples of US adults ≥18 years. Methods. Data were drawn from 2007 and 2012 National Health Interview Surveys (’s = 20127 and 30672, resp.). Logistic regression was used to examine associations of FMS to NVNM use (past 12 months) and evaluate potential modifying influences of gender and comorbidities. Multivariate models adjusted for sampling design, …

Decellularization Strategies For Regenerative Medicine: From Processing Techniques To Applications, Anna Gilpin, Yong Yang

Faculty & Staff Scholarship

As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide alternative strategies for treatment. One of the most promising techniques for tissue and organ regeneration is decellularization, in which the extracellular matrix (ECM) is isolated from its native cells and genetic material in order to produce a natural scaffold. The ECM, which ideally retains its inherent structural, biochemical, and biomechanical cues, can then be recellularized to produce a functional tissue or organ. While decellularization can be accomplished using chemical and enzymatic, physical, or combinative methods, each strategy …

Winter Grazing In A Grass-Fed System: Effect Of Stocking Density And Sequential Use Of Autumn-Stockpiled Grassland On Performance Of Yearling Steers, Domingo J. Mata-Padrino, E E D Felton, W B. Bryan, D P. Belesky

Faculty & Staff Scholarship

Winter grazing can help reduce the need for purchased feeds in livestock production systems, when finishing cattle on pasture. Our objective was to evaluate the influence of stocking density and grazing stockpiled forage on performance of yearling steers during winter. Three grasslands were winter grazed for two years: I, naturalized pastureland, and II and III, sown and managed for hay production during the growing season but grazed in winter. Two stocking densities were used: low 7.41 and high 12.35 steers ha−1. Herbage mass was estimated before and after each grazing event, and disappearance (consumption, weathering, and trampling) was the difference …

Stabilization Of Hif-1Α And Hif-2Α, Up-Regulation Of Mycc And Accumulation Of Stabilized P53 Constitute Hallmarks Of Cns-Pnet Animal Model, Sergey Malchenko, Simone T. Srendni, Yingtao Bi, Naira V. Margaryan, Jerusha Boyineni, Indra Mohanam, Tandra Tomita, Ramana V. Davuluri, Marcelo B. Soares

Faculty & Staff Scholarship

Recently, we described a new animal model of CNS primitive neuroectodermal tumors (CNS-PNET), which was generated by orthotopic transplantation of human Radial Glial (RG) cells into NOD-SCID mice’s brain sub-ventricular zone. In the current study we conducted comprehensive RNA-Seq analyses to gain insights on the mechanisms underlying tumorigenesis in this mouse model of CNS-PNET. Here we show that the RNA-Seq profiles derived from these tumors cluster with those reported for patients’ PNETs. Moreover, we found that (i) stabilization of HIF-1α and HIF-2α, which are involved in mediation of the hypoxic responses in the majority of cell types, (ii) up-regulation of …

Nano-Magnetic Resonance Imaging (Nano-Mri) Gives Personalized Medicine A New Perspective, Lorenzo Rosa, Jonathan Blackledge, Albert Boretti

Faculty & Staff Scholarship

This paper reviews some of the major and most recent advances in nanoscale-magnetic resonance imaging (nano-MRI) for personalized medicine (PM). Nano-MRI may drastically expand the capabilities of the traditional magnetic resonance images (MRI), down to the nanometer scale and possibly, in the near future, at the atomic scale. Nano-MRI is potentially able to observe structures which cannot be seen using today’s molecular imaging, with sensitivities of many billions of times better than MRI as currently used in hospitals, for example. The paper briefly reports on the foremost research themes in nano-MRI.

Amnesia Associated With Bilateral Hippocampal And Bilateral Basal Ganglia Lesions In Anoxia With Stimulant Use, Marc W. Haut, Jeffery P. Hogg, Patrick J. Marshalek, Blair C. Suter, Liv E. Miller

Faculty & Staff Scholarship

We report a case of a 55-year-old man with ischemic lesions of the bilateral hippocampus and bilateral basal ganglia following a myocardial infarction during an episode of multiple drug use with subsequent anoxia requiring resuscitation. He presented for a neuropsychological evaluation with an anterograde amnesia for both explicit and procedural memory. There are two main points to this case, the unique aspects of the bilateral multifocal lesions and the functional, cognitive impact of these lesions. We hypothesize that his rare focal bilateral lesions of both the hippocampus and basal ganglia are a result of anoxia acting in synergy with his …

Agonist Muscle Adaptation Accompanied By Antagonist Muscle Atrophy In The Hindlimb Of Mice Following Stretch-Shortening Contraction Training, Erik P. Rader, Marshall A. Naimo, James Ensey, Brent A. Baker

Faculty & Staff Scholarship

Background: The vast majority of dynamometer-based animal models for investigation of the response to chronic muscle contraction exposure has been limited to analysis of isometric, lengthening, or shortening contractions in isolation. An exception to this has been the utilization of a rat model to study stretch-shortening contractions (SSCs), a sequence of consecutive isometric, lengthening, and shortening contractions common during daily activity and resistance-type exercise. However, the availability of diverse genetic strains of rats is limited. Therefore, the purpose of the present study was to develop a dynamometer-based SSC training protocol to induce increased muscle mass and performance in plantarflexor muscles …

Lhx8 Interacts With A Novel Germ Cell-Specific Nuclear Factor Containing An Nbl1 Domain In Rainbow Trout (Oncorhynchus Mykiss), Liyuan Fu, Prasanthi P. Koganti, Jian Wang, Lei Wang, Cheng-Lun Wang, Jianbo Yao

Faculty & Staff Scholarship

Lhx8 is an important transcription factor that is preferentially expressed in germ cells. Lhx8 null mice are infertile due to lack of oocytes and impairment of the transition from primordial follicles to primary follicles. Lhx8 deficiency also affects the expression of many important oocyte-specific genes. In this study, we report the characterization of rainbow trout lhx8 genes and identification of a novel germ cell-specific nuclear factor that interacts with Lhx8. Two lhx8genes, lhx8a and lhx8b, were identified, encoding proteins of 344 and 361 amino acids, respectively. The two proteins share 83% sequence identity and both transcripts are specifically expressed in …