Digital Commons Network^™

Horizontal And Vertical Transmission Of Microbes Associated With Blow Flies Of Forensic Importance, Gabrielle Alisa Burton

Master of Science in Forensic Science Directed Research Projects

Entomological and microbiological evidence can be used in the estimation of postmortem intervals (PMI). This study observes the horizontal and vertical transmission of the microbial community associated with blow flies of forensic importance. Variable region four (V4) of the 16S ribosomal DNA (16S rDNA) was amplified and sequenced from various life stages of blow flies (n=72) using dual-index high throughput sequencing strategy on the MiSeq FGx platform. No significant difference in bacterial community structure was observed between blowfly adults with carrion access and blowfly adults with no carrion access. Dysgonomonas, unclassified Proteobacteria, Escherichia, Ignatzschia, Providencia, …

Putting The “U” In Shore U: Expanding Postsecondary Education On Virginia’S Eastern Shore, Katelyn D. Jones, Karen Belanger, Katharine Wise, Takeya Mclaurin

Doctor of Education Capstones

The University of the Eastern Shore of Virginia Foundation (Shore U) submitted a Request for Assistance to examine opportunities to expand postsecondary education on Virginia’s Eastern Shore. In response to this request, a doctoral research team conducted a mixed-methods study that explored potential pathways for a four-year degree option on the Eastern Shore. Eastern Shore Community College (ESCC) students, ESCC employees, and Eastern Shore community members were surveyed during the quantitative phase of the study. Secondary data was used to understand the postsecondary positionality of Eastern Shore high school students. The President of ESCC, Eastern Shore community members, and Virginia …

Unmet: The Undergraduate Experience Of Basic Need Insecurity At An Uncommon University, Michelle C. Bair, Rachelle V. Blackwell-Stegall, Sara E. Rafuse, Ra’Meer J. Roberts, Sara E. Weinstein

Doctor of Education Capstones

Virginia Commonwealth University’s (VCU) Dean of Students office submitted a Request for Assistance with needs related to food and housing insecurity and lack of basic funds for students. To address this request, a doctoral Capstone team conducted a problem and context analysis, literature review, student focus groups, and an internal survey of departments. The goal was to explore the undergraduate experience of unmet needs and to identify gaps and overlaps in basic needs support and services already provided at VCU. Findings suggest that students do not know the resources available to them through the Dean of Students office, that students …

Driven By Personality: Exploring The Relationship Between Custom Cars And Self-Expression, Tharwa Dalansi

Theses and Dissertations

Custom Car Culture (CCC) is a form of creativity that emerges from personal identities and passions. It involves the restoration, modification and personal adornment of commercially manufactured cars. This process creates a journey, where the enthusiast goes through multiple phases of self-reflection. But due to a lack of local resources, Qatar-based CCC enthusiasts often have to send their cars abroad to Dubai or Japan in order to customize them, relinquishing creative control in the process.

In response to these challenges, based on observations and interviews conducted among actual CCC enthusiasts, I develop a platform for engagement between these enthusiasts and …

Exploring The Relationship Between The Epigenome And Metabolism In Mycn-Amplified Neuroblastoma, Krista M. Dalton

Theses and Dissertations

High risk neuroblastoma (NB) is responsible for nearly 15% of all cancer related deaths in the pediatric population. The transcription factor MYCN is the most studied oncogene in NB, automatically conferring high-risk and has been found to be difficult to target. One way that MYCN amplification in NB functions to support tumorigenicity is through altered metabolism, satisfying the demand for rapid proliferation by increasing the nutrient flux through biosynthetic pathways. We performed an unbiased screen on select metabolic targeted therapy combinations and correlated sensitivity with over 20 subsets of cancer. We found that MYCN-amplified NB is hypersensitive to the …

Designing An Eco-Resilience Community In Brentwood, Washington D.C., Nadya Syazsa

Master of Urban and Regional Planning Capstone Projects

As the world urbanizes at a dangerously rapid pace, this Professional Plan helps with addressing the need for urban resilience – the ability of a city’s systems to withstand and adapt to the shocks and stresses it may endure, such as natural disasters.

The purpose of this Plan is to design a network of green infrastructure projects (GIs) as part of establishing an eco-resilient community in Brentwood, Washington D.C. These projects are meant to be low-impact developments (LIDs) in order to minimize disruption to the existing fabric of the Brentwood community, yet still aid residents by increasing their capacity to …

Developing Agrihoods: The Context For Petersburg, Brandon L. Archer

Master of Urban and Regional Planning Capstone Projects

Petersburg has an extensive history of navigating through challenging times. From its inception, African Americans have played an important role in the development of the city, and today that spirit is still present. As evidenced by an extensive network of community support and stakeholders, the Petersburg Youth Farm has matured into a resource for not just the residents of the neighborhood but of the city as a whole. By incorporating some innovative planning techniques and building a housing development truly dedicated to community needs, we can improve lived experiences in the city.

By adopting the recommendations set forth in this …

Revitalization Strategies For Downtown Luray, Va, Trinity L. Waldron

Master of Urban and Regional Planning Capstone Projects

The Town of Luray seeks a better understanding of its foundational challenges that contribute to off-season economic growth and development. This plan examines existing conditions to identify existing assets and missing linkages that can improve downtown economic development. Data analyzed through market analysis will provide information in examining spending patterns and business potential.

Vcu And The 2021 Virginia Course Materials Survey, Jessica Kirschner

Scholarship about Open Education

In Fall 2021, VIVA, Virginia’s Academic Library Consortium, distributed the Virginia Course Materials Survey to a selection of students at Virginia higher education institutions. The survey hoped to explore:

1. The relationship between educational equity and textbook costs, and

2. Which textbook features most benefited students’ learning experiences.

This report presents the results from this survey, focused on Virginia Commonwealth University (VCU). At VCU, the survey was disseminated to a 20% random sample of students. 603 VCU responses were received (10% response rate). Due to the IRB approved protocols and efforts to maintain respondent anonymity, VCU data is only available on the …

The Clinical Inquiry Process Diagram Question Development Checklist, Roy E. Brown

VCU Libraries Faculty and Staff Publications

The Clinical Inquiry Question Development Checklist is a handout used in instructional sessions with nurses about the clinical inquiry process. This process includes evidence-based practice, performance improvement, and research. The revised handout closely aligns with the 4^th version of the Clinical Inquiry Process Diagram and contains information related to the performance improvement process and various other aspects of the clinical inquiry process.

Addressing School Bullying Since The Onset Of Covid-19: A Merc Research And Policy Brief, David Naff, Morgan Meadowes, Kim Dupre, Alicia Gaston, Fatemah A. Khawaji, Christina Tillery, Makeba Lindsay D'Abreu, Lauren Powell, Deanna Fierro

MERC Publications

Among the challenges presented by the COVID-19 pandemic is a shift in the nature and intensity of school bullying, perhaps symptomatic of the rapid changes and stressors that PK-12 students have endured since 2020. In this MERC research and policy brief, we explore how school bullying has changed since the onset of COVID-19 as well as research-based strategies for how educators and division leaders can best respond to it. The brief answers the following questions: 1) What is the nature of school bullying since the onset of COVID-19? 2) Which students have been particularly impacted by bullying since the pandemic? …

Emergent Trends Of Contemporary Dramatic Recontextualization: An Exploration Utilizing Eugene O'Neill's Mourning Becomes Electra, Cameron M. Nickel

Theses and Dissertations

The art of adaptation in the realm of drama has undergone an easily recognizable evolution in the past couple of decades, from the work of Sarah Ruhl to Branden Jacobs-Jenkins. This evolution has opened doors to an altogether new form of adaptation in the theatre: dramatic recontextualization. While the two forms are built upon a foundation of shared aspects, there are certain observable and quantifiable delineations between the two artistic forms. As this trend continues to grow exponentially in the world of theatre, it is important to further research the origins and methodologies of contemporary dramatic recontextualization, both to provide …

The Use Of Silver Diamine Fluoride In Dentistry In Virginia, Jessica Eisenberg

Theses and Dissertations

Purpose: The purpose of this research was to determine the use of silver diamine fluoride (SDF) in dentistry in Virginia and the factors that affect the practitioners’ decision. Methods: A survey was sent to the members of the Virginia Dental Association (VDA) by email and posted on the VDA social media accounts. The survey questions measured the practitioner’s demographics, knowledge, and factors that affect their utilization of SDF. Results: Most providers were general dentists (77%). The most common clinical situations for SDF use were to arrest caries in children with behavioral issues (77%), to delay restorative treatment (73%), in medically …

The Wound Healing And Antibacterial Properties Of Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles, Emily N. Wandling

Theses and Dissertations

Treatments for acute respiratory distress syndrome (ARDS) are still unavailable and the prevalence of the disease has only increased due to the Covid-19 pandemic. Mechanical ventilation regiments are still utilized to support declining lung function, but they also contribute to lung damage and increase the risk of bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSC secretome and the extracellular matrix (ECM) into a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta-potential, and mass spectrometry …

Contribution Of Metabolic Cell Swelling On Microcirculatory Perfusion In Septic Shock, Charles E. Payne

Theses and Dissertations

Sepsis, a systemic immunological response that leads to organ-dysfunction, the development of septic shock, and death has mortality rate of 15-25%. A subset of sepsis, septic shock, has an in-hospital mortality rate of 30-50%. Sepsis and its treatment are the most expensive healthcare problem in the United States with the US Agency for Healthcare Research and Quality estimating annual costs over $20 billion. Hypoxia experienced in septic shock induces metabolic cell and tissue swelling leading to further microcirculatory dysfunction and poor tissue perfusion. Reversing metabolic cell swelling corrects these perfusion defects. Two models were used in rats to induce septic …

Pms And Gas Pollutants Removal By Electret Filter Media And Metal-Organic Frameworks (Mofs), Yu Zhang

Theses and Dissertations

Indoor air quality (IAQ) has been becoming a major concern worldwide because statistical data showed that the indoor concentrations of gas pollutants, e.g., volatile organic compounds (VOCs) and particulate matters (PMs) are often 2–5 times higher than that of outdoors. Especially, people spend 87% of their time indoors and increasing to more than 90% during COVID-19 pandemic. The high concentration of these indoor air pollutants is mainly due to the reduced air exchange rate for the design of energy-efficient building construction and the increased usage of furnishings, glues, paints, furnaces, etc., leading to VOC outgassing and particle emission. Evidence has …

Addressing Death Distress In Caregivers Of Patients With Malignant Glioma, Kelcie D. Willis

Theses and Dissertations

The field of psychosocial oncology seeks to improve the quality of life of patients and families affected by cancer. This task becomes increasingly important when there are limited, non-curative treatment options, a high probability of recurrence, and rapid functional decline, such as in the case of a malignant glioma (MG). Indeed, the diagnosis of a MG is associated with negative sequalae that often translates to higher caregiver burden. Caregivers—who must manage the functional decline of the patient, changes within the family, and a complicated medical system—often feel overwhelmed, isolated, and ill-equipped to handle these myriad responsibilities. While they also report …

Norteada - In The Greater Geologic Span Of Time, Just Fine, Clara Cruz

Theses and Dissertations

In this thesis I will spend some time talking to the dead. We’ll discuss callously mercurial and aquarian takes on things including LIVED TIME, DEATH, ART and VIBES. Keep in mind that this is just the beginning and these are just the bones - some fleshed out, some sucked to the marrow, some picked apart. Remind me that I will have forgotten at least half of what I meant to write and lost my keys three times before we’re done.

Irene Cox, Lindsey Arturo

Theses and Dissertations

This document explores the question of “Who is Irene Cox?” as a jumping off point for creating an artwork which navigates online information landscapes and their logics circa 2023. By using thirteen unique but similar found paintings of the same floral still life by an artist named “Irene Cox” I create a visually meandering, absurd, haunting, and comical pathway for thinking through who exactly is Irene Cox via the internet. How such a question is even asked becomes the art in which a myriad of visual techniques are used to explore the nature of the question. Included in this paper …

A Constructed Memoir: The History And Heritage Of The Baloch Community In Qatar, Somaia Nahang Dorzadeh

Theses and Dissertations

In the 1960s, when my father was only thirteen years old, he took a perilous journey on a boat alone from Sistan va Baluchestan, Iran, to Qatar, in the hopes of a better life. Like many long-term residents from Balochistan, my father has lived in Qatar for decades, predating the establishment of the modern state of Qatar itself in 1971. His legal position in the country today remains subject to the Kafala system, and his residency is subject to his employment status, which must be renewed each year. In other words, permanency is never guaranteed, nor is future planning.

In …

Trans* Streamers On Twitch.Tv: The Intersections Of Gender And Digital Labor, R. Lawson

Theses and Dissertations

Twitch.tv is a live entertainment platform where individuals live stream events, including playing video games, playing board and tabletop games, creating art, and more. Twitch has a diverse base of streamers, but Twitch has just begun. The most common approach has focused on cisgender, heterosexual white men in the cases where it has been studied. Though these streamers should be studied in sociology, this focus leaves out the experiences of both cis women and Trans* streamers. This research proposal tries to situate the relationship of Trans* streamers with both the platform and their audience, seeing if these relationships affect their …

Half Of Two Hungers, Aida A. Lizalde Rios

Theses and Dissertations

Abstract

half of two hungers is an extension of my sculptural practice; a weaving of the ambiguous borders of memory, trauma, disease, identity, assimilation, survival, spirituality, love, erotics, and desire. It is a psychological and sensorial landscape, where I travel consciously and subconsciously to pull shapes and material experiences out into the world of bodies and objects.

Investigation Of Sneathia Vaginalis And The Host Antibody Response To S. Vaginalis Pore-Forming Toxin, Cpta, In Vivo, Zion T. Mccoy

Theses and Dissertations

Sneathia vaginalis, formerly Sneathia amnii, is a gram-negative vaginal species that is associated with pregnancy complications including preterm birth. The only virulence factor of S. vaginalis that has been characterized is the cytopathogenic toxin, component A (CptA), a pore-forming toxin that lyses human red blood cells and human epithelial cells and plays a role in traversal of the bacteria through fetal membranes. The overarching goal of this work was to examine the host immune response to CptA. To investigate the immune response to CptA, levels of anti-CptA IgM, IgA, and IgG in human mid-vaginal swab samples obtained during …

A Tenth Of A Second, Natalia Andrea Mejía Murillo

Theses and Dissertations

A Tenth of a Second is an installation that explores modes of cartographic representation of the universe, specifically the moon, in dialogue with poetic language. The work is based on research and reflection on disciplines such as astronomy and archaeology and addresses issues such as the fragility of the reference and categorization systems we use to approach reality, tactility and the body as a standard of measurement.

The Game Of Absolute, Rupeng Zhao

Theses and Dissertations

My thesis paper writing is a collection of short stories. Or it could be thought of as a small magazine or even an instruction manual for my work. There is a loose correspondence between its content and the symbols used in my installations. It is also realistically and physically presented in my exhibition as part of a "game box". The chaotic and contradictory narrative of this collection of short stories stems from my exhaustive pursuit of the elements that I try to approach in each story. This insatiable pattern of compulsive organization is also evident in the content of my …

Treponema Denticola Synthesizes C-Di-Amp And Encodes The Cdaa-Type Diadenylate Cyclase Cdaa, Claire R. O'Brien

Theses and Dissertations

Periodontitis is a form of oral disease characterized by dysbiosis of the oral microbiome, leading to inflammation, bone resorption, and in severe cases, entire tooth loss, affecting 42% of adults in the US. One of the bacteria most associated with periodontal disease progression is Treponema denticola (Td), an oral spirochete which inhabits the mouth in small quantities during health but which can dominate the biofilms that form during periodontal disease. The ability of Td to survive in a disease environment and contribute to the progression of disease requires the use of robust signaling networks. Analysis of Td cultures …

Role Of Histone Deacetylase (Hdac) In Epithelial To Mesenchymal Transition (Emt) In A Human Cholangiocyte Model Of Ischemic Cholangiopathy, Priyanshi Pragnesh Parikh

Theses and Dissertations

Organ transplants are a vital intervention for many diseases that result in end stage organ failure. Currently, the donation pool is not meeting the demands of the transplant list. Expanding this pool to include donation after cardiac death (DCD) is highly sought-after. However, the use of DCD livers can lead to increased odds of graft failure and ischemic cholangiopathy. The loss of epithelialization and fibrosis that occurs during ischemic cholangiopathy is characteristic of these cells undergoing epithelial-to-mesenchymal transition (EMT). The biological changes the cell experiences enhance migratory capacity, invasiveness, and increased resistance to apoptosis. Our earlier studies have shown differential …

Motivations Of Adult Patients Seeking Orthodontic Retreatment, Scott V. Philips

Theses and Dissertations

Objective: The aims of this study were (1) to compare the reasons adult patients seek orthodontic first-time treatment to the reasons adult patients seek orthodontic retreatment, and (2) to compare the concerns, motivations, and satisfaction levels of adult patients seeking orthodontic retreatment across the different previous treatment provider types of orthodontist, general dentist, and Direct-to-Consumer company.

Methods: At their initial orthodontic visit, adult patients (N=108) were invited to participate in the digital survey (REDCap) study. Subjects were recruited from the graduate orthodontics clinic at Virginia Commonwealth University and seven private orthodontic practices across the United States. Visual Analogue Scales were …

Characterization And Investigation Of Cold Atmospheric Plasma And Its Effects On Cancer Cell Biology, Thomas M. Ritrosky

Theses and Dissertations

Modern cancer treatment uses radiation therapy in over 50% of patient cases. It is an e↵ective way of treating tumors because the mechanisms of cell killing are well known through the damage that ionizing radiation does to DNA. The amount of radiation can be tracked through measuring the dose of the clinical photon or electron beam used. However, there are limitations in the usage of radiation therapy, for example, a tumor can create hypoxic areas that become radioresistant leading to complete ine↵ectiveness of further radiation treatment. This project looks into the application of cold atmospheric plasma as an adjuvant therapy …

Rooted In Between, Manavi Singh

Theses and Dissertations

Rooted in Between is a personal project that explores the complexities of belonging and the emotions that come with navigating between spaces that we call home. This written thesis is in support of the carefully curated video based and photographic work presented at an exhibition of the same title. The project's primary objective is to delve into the physical and emotional baggage that we carry with us as we move from one place to another. The project highlights the coping mechanisms adopted by the different generations in my family while struggling to find a place where we truly belong.