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Development, Implementation And Evaluation Of An Education Course For The Utah State University Coordinated Undergraduate Medical Dietetics Program, Cynthia Olson Obermiller May 1983

Development, Implementation And Evaluation Of An Education Course For The Utah State University Coordinated Undergraduate Medical Dietetics Program, Cynthia Olson Obermiller

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The entry-level clinical dietitian must possess competencies in the area of communications and in the process of education. Training and practice in these areas, as related to the profession of dietetics, must be provided during the professional phase of the dietetic student's training. The purpose of this study was to develop, implement and evaluate a course for the Coordinated Undergraduate Medical Dietetics Program at Utah State University. This course, entitled Education in Clinical Dietetics, was added to the junior year fall quarter curriculum. The ten-week course combined didactic and lab experiences to provide the students with a thorough background in …


Longitudinal Pantothenic Acid Status Of Pregnant And Lactating Women, Won Oack Song May 1983

Longitudinal Pantothenic Acid Status Of Pregnant And Lactating Women, Won Oack Song

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pantothenic acid nutritional status was evaluated in a cohort of twenty-nine Caucasian pregnant women, aged 20-35, during their third trimester of pregnancy, at two-weeks postpartum and at three months postpartum. Seventeen non-pregnant, non-lactating women who had similar demographic backgrounds as the pregnant women were selected as a control group and followed the same experimental schedule as the pregnant women. A fasting blood sample, two days 24-h urine specimens and diet record were obtained for each of two days from each subject at each period.

The mean dietary pantothenic acid intakes of the women during pregnancy (5.30±1.74 mg/day) and during lactation …


An Evaluation Of Various Methods Of Roasting Whole Turkeys From The Frozen State, Kim Merida-Klemmedson Teot May 1983

An Evaluation Of Various Methods Of Roasting Whole Turkeys From The Frozen State, Kim Merida-Klemmedson Teot

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Methods of roasting frozen turkeys were evaluated to determine the optimum final internal temperature (71,77, or 82°C), the best low oven temperature method (foil tent vs roasting bag at 93,107, 121, and 135°c), and the best overall method (foil tent-121 °c, foil tent-163°C, roasting bag-163°c, foil wrap-232°C). The lower the final internal temperature the juicier and more tender the bird however, the 77°c final internal temperature is recommended because at the 71°c internal temperature, the thigh joint was unacceptably pink. Among the low oven temperature methods, birds roasted at 121°c received the highest average sensory scores. Birds roasted by the …


Meltability And Rheology Of Model Process Cheese Containing Acid And Rennet Casein, Paul Alexander Savello May 1983

Meltability And Rheology Of Model Process Cheese Containing Acid And Rennet Casein, Paul Alexander Savello

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Process cheese models were prepared by blending acid or rennet casein, milk fat, sodium chloride, 2.5% emulsifying salt and water and heating to 80 C. Acid casein cheese models were subjected to sodium hydroxide conditioning at 65 C in the cooker. Model process cheeses were acidified with lactic acid and treated by addition of undenatured and heat-denatured whey protein, four different emulsifying salts and sodium oxalate.

Meltability and toughness of the model cheese increased to a maximum with increased sodium hydroxide conditioning of acid casein to pH 7.20. These same properties decreased with addition of undenatured and heat-denatured whey protein …


Enzymic Milk Coagulation: Casein Micelle Aggregation And Curd Formation, Donald J. Mcmahon May 1983

Enzymic Milk Coagulation: Casein Micelle Aggregation And Curd Formation, Donald J. Mcmahon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Enzymic milk coagulation was monitored by measuring changes in curd firmness and apparent absorbance of undiluted milk. Detection of coagulation, visually or rheologically, occurred after the milk changes from a system of aggregating particles to an extended space network. This change was observed as a shoulder in apparent absorbance plots and coagulation time was defined as the critical point in the aggregation process analogously to non-linear condensation polymerization reactions. It corresponds to the inflexion point during the period when apparent absorbance was rapidly increasing and can be calculated by fitting curd firmness data to an exponential equation.

Addition of calcium …


A Method For Separating Casein Micelles From Whey Proteins For Determining Casein In Milk, Robert N. Carpenter May 1983

A Method For Separating Casein Micelles From Whey Proteins For Determining Casein In Milk, Robert N. Carpenter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine if size exclusion chromatography could be used to separate casein micelles from whey proteins for a rapid, direct test to measure percent casein in milk. A size exclusion chromatography column was developed for the separation having dimensions 100 by .4 cm. Packing material selected was glycophase coated porous glass supports. A Beckman DU-8B spectrophotometer monitored the casein and whey protein peaks as they eluted and a Tektronix 4052 computer accepted data points every 4 sec, storing these on tape. Absorbances and areas of each peak were used in the evaluation of samples. …


The Effect Of Long Term Storage Of Nonfat Dry Milk On Sensory Quality And On The Development Of Oxidative Derivatives Of Sterols, Nancy R. Driscoll May 1983

The Effect Of Long Term Storage Of Nonfat Dry Milk On Sensory Quality And On The Development Of Oxidative Derivatives Of Sterols, Nancy R. Driscoll

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nonfat dry milk (NOM) was evaluated to determine the effect of long term storage on its sensory quality, its solubility index and on the development of oxidative derivatives of sterols. Storage variables included the form of the milk, the atmosphere in the storage container, the type of package and the storage temperature. A trained taste panel evaluated the milk for the development of cooked, metallic, and oxidized/stale flavors, mouthfeel and overall acceptability. Gas chromatography was used to determine cholesterol content and the quantity of oxidative derivatives of cholesterol present in the samples.

Although long term storage of NOM did not …


Relationships Between Psychogenic Needs And Theoretical Frameworks Of Psychotherapists, Dennis E. Ahern May 1983

Relationships Between Psychogenic Needs And Theoretical Frameworks Of Psychotherapists, Dennis E. Ahern

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Each psychotherapist has a personal theoretical framework, that is, a set of assumptions on which his or her psychotherapy is based. It appears from the published writings and opinions of therapists that therapists generally concur that a major factor in the formation of an individual therapist's theoretical framework is the therapist's personality. This study was designed to address this issue by examining the relationship between the psychogenic needs and theoretical framework of the therapist.

From an accessible population of 178, responses were obtained from 153 therapist participants (108 males and 45 females) from five Utah training programs and three experience …


An Analysis Of The Hiskey Nebraska Test Of Learning Aptitute For Navajo Children With Suspected Learning Difficulties, Susan Louise Sawyer May 1983

An Analysis Of The Hiskey Nebraska Test Of Learning Aptitute For Navajo Children With Suspected Learning Difficulties, Susan Louise Sawyer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of the study was to evaluate the appropriateness of the Hiskey Nebraska Test of Learning Aptitude as a measure of ability for Navajo students with suspected learning difficulties. Item analytic procedures were employed for the 61 students in the younger group (ages 5-10) and for the 57 students in the older group (ages 11-17).

Using Hoyt's analysis of variance routine, reliability estimates of .87 (younger group) and .81 (older group) were obtained. The majority of the items discriminated in the appropriate direction for both subtest and total test scores.

It was concluded that the Hiskey Nebraska Test of …


Social Interaction: The Relationship Between Facial Attractiveness And Verbal Influence Style, Laurie Jean Lee May 1983

Social Interaction: The Relationship Between Facial Attractiveness And Verbal Influence Style, Laurie Jean Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine if male and female communicators utilize different styles of persuasion with an attractive versus an unattractive female target. Forty undergraduate students were asked to persuade a female confederate in either an attractive condition or an unattractive condition to eat M&M's. Perceptions of attractiveness and personality assessments were checked by a post-experimental questionnaire. Influence attempts were rated and categorized by the use of the Social Interaction Scoring System. Individual responses were then factor analyzed to identify profiles of persuasive communication. These behavioral profiles were then statistically compared across experimental conditions by analyses of …


Stages Of Contemplative Mysticism: A Description And Taxonomy, Kenneth Burton Kaisch May 1983

Stages Of Contemplative Mysticism: A Description And Taxonomy, Kenneth Burton Kaisch

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to describe and classify the stages of contemplative mystical development in the major world religions. To accomplish this task, a sample of five major world religions was chosen from the total population. From each religion, a contemplative author who was regarded as an authority by the members of that religion was chosen. The major work of each author was described in order to make explicit his/her conceptualization of the stages of contemplative development. These conceptualizations were then compared together, resulting in two models of contemplative development. The first model was composed of those stages …


Jacobsonian Versus Autogenic Relaxation Training: Interactions With Locus Of Control, Mark J. Weaver May 1983

Jacobsonian Versus Autogenic Relaxation Training: Interactions With Locus Of Control, Mark J. Weaver

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to evaluate the thesis that matching locus of control with type of relaxation training enhances physiological self-control. This was accomplished by comparing the effectiveness of a somatic and a cognitive relaxation method for internal and external locus of control subjects. It was hypothesized that the two techniques vary in degree of cue salience, and that the attentional strategy of internals would interact with the cognitive approach while externals would respond better to the somatic approach.

Subjects were 80 volunteers from stressful occupations who scored high on the State-Trait Anxiety Inventory A-trait scale. Forty internals …


The Prediction Of Tenure And Job Performance Based On The Job Activity Preference Questionnaire (Japq): A Concurrent Study, Nolan R. Roberts May 1983

The Prediction Of Tenure And Job Performance Based On The Job Activity Preference Questionnaire (Japq): A Concurrent Study, Nolan R. Roberts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to assess the concurrent validity of the JAPQ in predicting the work output and tenure levels of persons employed in the occupation of Data Entry Operator-Financial Keyer. Three separate hypotheses were tested: (1) JAPQ-D2 differences based on employee tenure and output loads; (2) JAPQ dimension preference differences, which may not be reflected in JAPQ-D2 scores; and, (3) the relationship between employee tenure and employee output. Separate research questions focused on the applicability of the JAPQ in predicting employee tenure and employee output, based on multiple regression results.

Sixty financial keyers were administered …


Rhizosphere N2 Fixation In A Forest Ecosystem In Situ Assays And Evaluation Of The Acetylene Reduction Technique, Inger Börjesson May 1983

Rhizosphere N2 Fixation In A Forest Ecosystem In Situ Assays And Evaluation Of The Acetylene Reduction Technique, Inger Börjesson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In situ assays of N2 fixation activity, using the acetylene reduction technique, were performed in four successional stages of a Northern Wasatch Mountain subalpine forest ecosystem, elevation 2,800 m. Emphasis was made on rhizosphere fixation in association with Antennaria microphylla and Achillea millefolium. The vegetation period was approximately 100 days.

Assays were performed in Saran bags. A defined amount of propane was injected at initiation of the assay and acetylene was generated from CaC2. Samples were analyzed for ethylene and propane. Data were evaluated assuming that the ethylene production was directly proportional to the increase in …


Predicting Success In College Mathematics From High School Mathematics Preparation, Richard A. Shepley May 1983

Predicting Success In College Mathematics From High School Mathematics Preparation, Richard A. Shepley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a model to predict the college mathematics courses a freshman could expect to pass by considering their high school mathematics preparation. The high school information that was used consisted of the student's sex, the student's grade point average in mathematics, the highest level of high school mathematics courses taken, and the number of mathematics courses taken in high school.

The high school sample was drawn from graduated Seniors in the State of Utah for 1979. The college sample was drawn from the fall semester 1980 at Utah State University, Weber State College, …


History Of The Forty-Second Parallel As A Political Boundary Between Utah And Idaho, Nancy Bergeson May 1983

History Of The Forty-Second Parallel As A Political Boundary Between Utah And Idaho, Nancy Bergeson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The original purpose of this paper was to discover why Cache County, Utah at one time taxed towns now located in the State of Idaho. Later, it became apparent that a history of the forty-second parallel was necessary to fully understand the reasoning used by both the Federal and local governments in setting up the political boundaries of Utah and Cache County. Therefore, it was necessary to research the records of the Federal Government, Cache County Government, the LDS Journal History, and diaries of residents of Cache and Bear Lake Valleys, as well as detailed accounts of Spanish and Mexican …


Natural Killer Activity In Gardner's Syndrome, Ann Marie Stembridge May 1983

Natural Killer Activity In Gardner's Syndrome, Ann Marie Stembridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Gardner's syndrome is an autosomal dominant disease presenting multiple colonic polyps with a predisposition for malignant change. In addition to colonic polyp formation by early adolescence, extracolonic lesions appear often prior to polyp formation. One theoretical mechanism for the origin of polyps and malignancies in Gardner's syndrome is a genetic defect in the natural killer cell activity of patients with this disease. Natural killer cells are a subpopulation of lymphocytes that spontaneously lyse tumor cells and virally transformed cells. A study was undertaken to determine the natural killer activity of patients with Gardner's syndrome.

A technique termed chromium release was …


Evaluating The Design And Operation Of Irrigation Canals In Egypt, Amany El Kayal May 1983

Evaluating The Design And Operation Of Irrigation Canals In Egypt, Amany El Kayal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A computer simulation model for temperature data was developed in order to generate synthetic daily temperature. The model consists of two components: a deterministic component represented by a Fourier series fit, and a stochastic component represented by an autoregressive model. The simulation model was proven to be able to preserve the main statistics of the historical series.

The data generated by the above temperature model were used as an input to an irrigation water requirements simulation model. This model incorporates the probabilistic nature of these requirements. The irrigation system of Abo Raya area in Egypt was used to demonstrate the …


Electronic Feeding Doors For Individual Feeding Of Group Housed Sheep Compared To Individual Housing And Feeding Or Group Housing And Feeding, Ronald J. Lane May 1983

Electronic Feeding Doors For Individual Feeding Of Group Housed Sheep Compared To Individual Housing And Feeding Or Group Housing And Feeding, Ronald J. Lane

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirty-six mature purebred cheviot ewes and 36 polypaysired wether lambs were used in two separate experiments to determine the usefulness of an electronic feeding door as contrasted with traditional feeding situations. The sheep were stratified by weight into three management categories; group housing and feeding (G+G), individual housing and feeding (1+1), or group housing and individual feeding (G+1) using an electronic feeding door. The weight stratification formed a light and a heavy weight class resulting in a 3x2 factorial design. Two different training procedures were used to arrive at a generalized 14-day training sequence that does not require physical handling …


Locoweed Poisoning In Cattle: An Overview Of The Economic Problems Associated With Grazing These Ranges, John E. Barnard May 1983

Locoweed Poisoning In Cattle: An Overview Of The Economic Problems Associated With Grazing These Ranges, John E. Barnard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Locoweed poisoning, caused by ingestion of certain species of Astragalous and Oxytropis, has had serious economic impacts through a loss of productivity in livestock. This study has attempted to evaluate losses suffered by livestockmen grazing their cattle on areas infested with locoweed species. The results indicate a serious economic impact on these individuals.

Personal interviews were carried out with five cattle ranchers faced with typical locoweed problems. These beef cattle operations were located in Utah, Wyoming, and New Mexico. All of these producers described similar problems and losses due to locoweed poisoning. Information obtained from these interviews was used …


A Linear Programming Analysis Of Several Determinants Of Profit On A Simulated Northern Utah Dairy Farm, Jay Dee Atwood May 1983

A Linear Programming Analysis Of Several Determinants Of Profit On A Simulated Northern Utah Dairy Farm, Jay Dee Atwood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of the study was to analyze several determinants of profit on a simulated northern Utah dairy farm. A linear programming model was developed to accomplish this. Optimal conditions on the farm were estimated and then other model applications were made to evaluate the importance of milk price, cropland ownership, and corn silage production and feeding. The significance of cow quality also was estimated.

Optimal conditions on the farm gave rates of return to capital between savings rates and loan rates obtainable at a financial institution. Marginal values derived for land did not support the current asking price of …


The Economics Of Information And Spatial Price Behavior: An Empirical Assessment Of Producer's Cattle Prices In The Western Region Of The Usa: 1973-1981, Maria Lourdes Del Rosario Juan May 1983

The Economics Of Information And Spatial Price Behavior: An Empirical Assessment Of Producer's Cattle Prices In The Western Region Of The Usa: 1973-1981, Maria Lourdes Del Rosario Juan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study is an empirical verification of the theories of the economics of information at the cattle producer's market in the U.S. Western Region. Weekly data on producer's price quotations were obtained from CATTLEFAX for 1973 through 1981. The three major objectives of the research are: first, to determine whether price dispersion exists in the cattle producer's market in the Western Region: second, if significant price dispersion are shown to exist, to determine the nature of the regional price distribution; and third, if price dispersion do exist, to determine the implications of the dispersion relative to competitive structure, efficient informational …


Mule Deer Reproduction And Survival In The Lasal Mountains, Utah, Randall B. Smith May 1983

Mule Deer Reproduction And Survival In The Lasal Mountains, Utah, Randall B. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mule deer (Odocoileus hemionus) reproduction and survival on the LaSal Mountains, Utah were studied from 1978-81. Reproductive rates were high, suggesting that summer and fall forage quality was adequate and was not responsible for low recruitment observed in recent years. The combined fetal rate, determined from laparotomies and carcasses, was 1.72 for all females older than 1 year (N = 114). Fetal rates were 1.15 for yearlings (N = 20), 1.80 for prime females (2-7 years, N = 78), and 1.75 for old females (8+ years, N = 16). No evidence of breeding was found among fawns (N …


Growth Of Targhee And Targhee Crossbred Lambs On Utah Rangelands, Holly Ann George May 1983

Growth Of Targhee And Targhee Crossbred Lambs On Utah Rangelands, Holly Ann George

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Growth data were collected on 1848 Targhee and Targhee crossbred lambs from the Utah Agricultural Experiment Station flock at Cedar City for 1981 and 1982. The study objectives were to establish growth curves for seven lamb genotypes and examine within genotype growth differences among four grazing treatments (sheep alone, sheep and cattle mixed, both continuous and rotation).

Body weights were measured at birth, and at mean ages of 56, 120, and 154 (weaning) days of age. Linear, quadratic, and cubic regressions of weight on age were used to establish growth curves. Among genotype comparisons of entire growth curves were impossible …


Cell-Mediated Immunity Of The Dog Lung And Effects Of Inhaled 239puo2 Or Pulmonary Tumors, Jennifer Baker Galvin May 1983

Cell-Mediated Immunity Of The Dog Lung And Effects Of Inhaled 239puo2 Or Pulmonary Tumors, Jennifer Baker Galvin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cell-mediated immunity (CMI) in the lung has not been well characterized due to the lack of applicable tests. A major objective was to define pulmonary CMI serially in immunized and control lung lobes using the leukocyte procoagulant (LPCA) assay in young adult dogs. This was compared to a standard, but less reproducible CMI assay, macrophage migration inhibition facet (MIF). The CMI response, as measured by the LPCA assay, peaked in the blood 7 days after lung immunization. The pulmonary CMI response measured with cells obtained by bronchial lavage from the immunized and control lung lobes peaked at 9 to 12 …


Investigation Of Selected Aspects Of Kokanee (Onchorhynchus Nerka) Ecology In Porcupine Reservoir, Utah, With Management Implications, Paul Joel Janssen May 1983

Investigation Of Selected Aspects Of Kokanee (Onchorhynchus Nerka) Ecology In Porcupine Reservoir, Utah, With Management Implications, Paul Joel Janssen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several aspects of kokanee (Onchorhynchus nerka) ecology were studied in the fluctuating, 80ha Porcupine Reservoir, Utah in order to determine optimum management strategies. In 1981, escapement was enumerated and estimates of egg deposition were made. An estimate of the resulting fry recruitment was also attempted and was successful up to the arrival of spring runoff. In 1982, escapement was enumerated and estimates of egg deposition were made, with subsequent estimates of egg deposition and percent survival to the advanced eyed stage. Age and growth rates were determined and survival rates estimated.

Escapement for 1981 and 1982 was 5,463 …


Development And Pathogenicity Of The False Root-Knot Nematode Nacobbus Aberrans And Its Relationship With Heterodera Schachtii And Meloidogyne Hapla On Sugarbeet, Renato Nunzio Inserra May 1983

Development And Pathogenicity Of The False Root-Knot Nematode Nacobbus Aberrans And Its Relationship With Heterodera Schachtii And Meloidogyne Hapla On Sugarbeet, Renato Nunzio Inserra

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Duration of embryogenic development of a Nebraska population of Nacobbus aberrans at 15 and 25 C were 51 and 9-10 days respectively. The first juvenile (J1) molted in the egg and the nematode emerged as J2. Kochia and sugarbeet root leachates did not significantly affect the percent of cumulative egg hatch. Temperature significantly affected juvenile emergence (P= 0.05), as less than 1, 5, and 20% of the eggs hatched at 5, 10 and 15 C, respectively. More than 70% of the eggs hatched at 20 and 25 C. At 25 C, postembryogenic development from egg to egg was …


Alteration In Serum Enzyme Levels, Activity Of Hepatic Monooxygenases, And Hepatocyte Morphology Due To Liver Enzyme Inducers In Microtus Montanus, Jeffrey Robert Hincks May 1983

Alteration In Serum Enzyme Levels, Activity Of Hepatic Monooxygenases, And Hepatocyte Morphology Due To Liver Enzyme Inducers In Microtus Montanus, Jeffrey Robert Hincks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Microtus sp. have been used in microcosm simulations of polluted environments. In this study, female Microtus montanus (mountain vole), 8 - 12 weeks of age, were pretreated with phenobarbital (PB), ß-naphthoflavone (BNF), or Aroclor 1254 (PCB) for 3 consecutive days. Doses ranged from 5 to 80 mg/kg for each pretreatment, while control animals received saline or corn oil only. The voles were sacrificed 1 day after the last dose of PB and 2 days after BNF and PCB. Hepatic microsomal enzyme activity of aniline hydroxylase (AH), p-nitroanisole-O-demethylase (PNA) and cytochrome C reductase (CCR) were measured in relation …


Hemoglobins Of The Cutthroat Trout Salmo Clarki, Jonathan N. Southard May 1983

Hemoglobins Of The Cutthroat Trout Salmo Clarki, Jonathan N. Southard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nine hemoglobins have been isolated from the blood of cutthroat trout. All nine hemoglobins bind oxygen cooperatively and appear to be tetramers with molecular weights of -64,000. The oxygen equilibria and subunit structures of the purified hemoglobins were studied. In addition, the red blood cells of cutthroat trout were examined for the presence of ATP and GTP, which are known to be physiological modulators of hemoglobins in fishes.

Five hemoglobins with isoelectric points from 9.1 to 7.0 are classified as cathodal hemoglobins. These five hemoglobins have identical oxygen binding properties by the criteria tested. All have oxygen equilibria which are …


Subunit Interactions In The Inducible Arginine Decarboxylase From Escherichia Coli B, Catalina N. Depusoy May 1983

Subunit Interactions In The Inducible Arginine Decarboxylase From Escherichia Coli B, Catalina N. Depusoy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The nature of the subunit interactions in the inducible arginine decarboxylase from Escherichia coli B is of considerable interest because of the observed differences in the catalytic activities of the dimer and the decamer; the decamer is active and the dimer is inactive. To study these interactions, inactive dimers were prepared by sodium borohydride reduction of the E-amino--pyridoxal-P Schiff base. Hybrid decamers were then prepared from varying molar ratios of native and reduced dimers. The hybrid decamers were indistinguishable from native decamers as observed in the analytical ultracentrifuge and on acrylamide gel electrophoresis. Kinetic studies indicated that true hybrids were …