Digital Commons Network^™

Viruses In Water, Nasrin Mohammadpour Daryaee

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

The use of water by man, plants and animals is universal. Without it there can be no life. Every living thing requires water. Man can live nearly two months without food, but can live only three or four days without water. In our homes, whether in the city or in the country, water is essential for cleanliness and health. Water can be considered as the principal raw material and the lowest cost raw material from which most of our farm produce is made. It is essential for the growth of crops and animals and is a very important factor in …

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments.

In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay …

Effects Of Dietary Levels Of Essential Fatty Acids On Rotaviral Gastroenteritis In Infant Mice, Kamyar Abolhassan Zahedi

Biology

Effects of dietary levels of essential fatty acids (EFA) on rotaviral gastroenteritis were studied using the infant mouse as the model animal. Female mice were placed on diets devoid of EFA (0%) for 30 days. Nine days prior to breeding they were divided into three groups receiving 25%, 100% and 200% of the normally recommended quantity of dietary EFAs and were kept on this diet throughout the studies. Two-day-old offspring were infected orally with cesium chloride-purified murine rotavirus (MRV) and the extent of infection was determined. The severity of diarrhea, as exhibited by greater ratios of intestinal weight to whole …

Cellular Immunity In Children With Down Syndrome (Trisomy-21), Roger Lee Noble

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Individuals with Down syndrome (US) suffer from increased incidence of respiratory infections and lymphoblastic leukemia, and a death rate that is particularly high in the first 5 years of life. Relatively few studies have probed immune parameters in young US children. Primary immune defects in DS may be masked by a degree of immune maturity in adults, and hygienic factors may have an effect on immune capability throughout the years. A study of young children can give clearer evidence of the actual primary immune defects in DS.

Blood samples were drawn from 20 DS children under 6 years old and …

Anthropometry, Metabolic Control And Dietary Intake Of Youth With Insulin-Dependent Diabetes Mellitus, Rochelle A. Follaco Clark

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to describe a sample of insulin-dependent diabetic youth in terms of selected anthropometric measurements, level of metabolic control and dietary intake. The 22 subjects (10 males and 12 females) were between the ages of seven to 16 years and, except for one female, regularly attended a multidisciplinary outpatient diabetes clinic.

The study involved cross-sectional observation of height, weight, triceps and subscapular skinfold measurements, mid-arm muscle circumference and weight:height ratios, and comparison of these measurements to national norms derived from non-diabetic controls. Levels of metabolic control were observed using glycosylated hemoglobin values, and examined for …

Foliar Penetration Of A Fungicide As Detected By Scanning Electron Microscopy And Energy Dispersive X-Ray Analysis, C. R. Krause

Scanning Electron Microscopy

CU II hydroxide, (Cu(OH)₂) a fungicide, produces injury as a result of "chemical burn" or phytotoxicity on some plants. Scanning electron microscopy and energy dispersive X-ray analysis were used to characterize chlorosis on red maple leaves following foliar applications of Cu(OH)₂. Copper was located with X-ray mapping in foliar mesophyll cells of trees sprayed with Cu(OH)₂ but not in leaves of trees sprayed with distilled H₂O.

The Utah Statesman, April 20, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Murine Rotavirus Infection: Characterization Of The Disease And Influence Of Host Malnutrition And Dietary Protein, Roger Lee Noble

Biology

A possible correlation between malnutrition and the increased incidence and severity of rotavirus-associated gastroenteritis in human infants was investigated using a mouse model originally described by others, but which was further defined and characterized as . a part of these studies. Two- or three-day-old specific-pathogen free Swiss Webster mice were orally infected with a 10% homogenate of intestines from mice infected with murine rotavirus. In the resulting disease, gross signs (diarrhea, alteration in intestinal appearance, failure to gain weight) and histologic changes typical of rotaviral gastroenteritis were manifest. Although quantification was not feasible by immunofluorescent staining, this technique did reveal …

Natural Killer Activity In Gardner's Syndrome, Ann Marie Stembridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Gardner's syndrome is an autosomal dominant disease presenting multiple colonic polyps with a predisposition for malignant change. In addition to colonic polyp formation by early adolescence, extracolonic lesions appear often prior to polyp formation. One theoretical mechanism for the origin of polyps and malignancies in Gardner's syndrome is a genetic defect in the natural killer cell activity of patients with this disease. Natural killer cells are a subpopulation of lymphocytes that spontaneously lyse tumor cells and virally transformed cells. A study was undertaken to determine the natural killer activity of patients with Gardner's syndrome.

A technique termed chromium release was …

Studies On Rotavirus Growth In Cell Culture And Various Aspects Of Replication, Scott M. Clark

Biology

The effect of trypsin on the infectivity and cultivation of rotaviruses has been studied. It was found that trypsin increases the infectivity of bovine, simian and porcine rotaviruses from 10 to 400-fold and that the enzyme increases the yield of bovine rotavirus in cell culture up to 1000-fold. Studies on the mechanism of trypsin enhancement of rotaviral infectivity indicate that the enzyme facilitates the uncoating of parental virus in the infected cell. Studies on the involvement of two particle types in bovine rotavirus replication indicate that the yield of light (L) infectious virions and dense (D) non-infectious particles in various …

Nucleotide Sequence Homology Between Rotaviruses, Jai Myung Yang

Biology

By coelectrophoresis in polyacrylamide gels, bovine, simian, and porcine rotaviruses exhibited different migration patterns in their double stranded RNA genome. There was less variation between bovine and simian rotavirus migration patterns than between bovine and porcine rotavirus patterns. Nucleotide sequence homology determined by hybridization between 3H labeled double stranded RNA and in vitro produced single stranded RNA showed 74 percent homology between bovine and simian rotaviruses, and 8 percent homology between bovine and porcine rotaviruses. The lack of a close relationship between bovine and porcine rotaviruses was confirmed by hybridization between 3H labeled, in vitro produced, single stranded RNA and …

Iron And Life, Thomas Emery

Faculty Honor Lectures

Iron, iron, everywhere but. . . . The plight of the ancient mariner surrounded by undrinkable water could hardly have been more frustrating than life on earth with respect to iron. Iron is the fourth most abundant element of the earth's surface, exceeded only by oxygen, silicon, and aluminum. Virtually all forms of life from the simplest bacteria to humans require iron to catalyze numerous and complex metabolic reactions. Because of the diversity and complexity of the role of iron in the life process, it has even been suggested that the origin of life on earth centered around the catalytic …

Effects Of Ddt Upon The Hematology And Immunology Of The Goldfish (Carassius Auratus), Maurice G. Zeeman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

DDT can cause hematological and immunological alterations in mammals and birds. Interference with normal hematological and immunological defense systems has been strongly linked to increased incidences of disease and tumors. Fish exposed to pesticides have been noted to be more susceptible to disease. This study examined DDT effects on goldfish hematological and immunological parameters.

Adult goldfish were kept in 250 liter aquaria at 25 ± 1° C. Fish were dosed intraperitoneally with p,p'-DDT in corn oil. Doses ranged from 0 to 500 mg/kg for acute (96 hour) hematology studies to 0 to 50 mg/kg for sub-acute hematology and immunology studies. …

Effects Of Pesticides On Immune Competency: Influence Of Methyl Parathion And Carbofuran On Immunologic Responses To Salmonella Typhimurium Infection, Anna M. Fan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Immunologic competence is correlated with resistance to infectious disease which may be affected by exposure to certain compounds. Methyl parathion (MP) and carbofuran (CF) have been reported to affect the development of active immunity in the rabbit and the present research was conducted to quantify the dosage relationships of these two pesticides to both host resistance and acquired resistance in the mouse.

Preliminary study was made on the virulence of Salmonella typhimurium in mice. Mortality rates among infected animals fed Purina Laboratory Chow diet providing 0.08, 0.7 3.0 mg MP/kg/day or 0.1, 0.6, 1.0 mg CF/kg/day along with untreated controls …

Subtle Teratogenic Effects Of Locoweed In Rats, Benjamin K. Nelson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Locoweed, a well-known teratogenic plant affecting livestock, is prevalent in mountain regions of the Western United States. Two common species (Astragalus lentiginosus and A. wootoni), administered to pregnant rats, induced behavioral deviations in their offspring. Treated mothers consumed less feed and gained less wieght during gestation than controls when gavaged locoweed at the rate of 1 gram whole plant per day on days 7 through 17 of gestation. A. lentiginosus reduced pup weight at birth (13% less than controls) and this weight reduction (as much as 29% less than controls) continued at least four weeks. A. lentiginosus also …

Partial Characterization Of The Genome Of Neonatal Calf Diarrhea Virus (Reoviruslike Agent), Bill B. Barnett

Biology

Electron microscopy, isopycnic centrifugation, thermal denaturation profiles and polyacrylamide gel electrophoresis were used to characterize the nucleic acid from neonatal calf diarrhea virus. The genome was shown to consist of 11 segments of double-stranded RNA. The molecular weights of the segments were determined to be: (in million dalton units) 2.18 ± .06, 1.73 ± .07, 1.64 ± .06, 1.48 ± .06, 0.94 ± .03, 0.77 ± .02, 0.50 ± .03, 0.50 ± .03, 0.50 ± .03, 0.29 ± .03, 0.22 ± .02. Six NCDV capsid polypeptides are described. The molecular weights of the capsid polypeptides ranged from 0.15 x 10 …

Food, Nutrition, And Health, Problems And Prospects, D. K. Salunkhe

Faculty Honor Lectures

Food, nutrition, and health - and their complex interrelationships - are necessities of life. Basically, nutrition depends on food; health depends on nutrition. Everyone needs to have enough good quality food to sustain himself. Satisfying that need on a world basis must be of concern to each of us.

Although accurate data are lacking, it is estimated that more than two-thirds of today's world population is afflicted by hunger and/ or malnutrition. Almost 300 million children are suffering physical and, probably, mental damage because of insufficient food. This evidence points to one of two conclusions: we have either too many …

The Production And Prevention Of Stomach Ulcers In Rodents, Eric H. Rudrud

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Rats given L-Ascorbic Acid in their drinking water prior to and during starvation did not develop severe ulceration in the glandular portion of their stomachs. Control rats which were either nontreated or given deactivated L-Ascorbic Acid developed severe stomach pathology on the starvation regimen.

The present study was based on the finding that food deprivation results in severe rumenal ulceration in rats. Given that L-AA is essential in maintaining tissue integrity and that ulcers are examples of tissue degeneration, the L-AA in large amounts could retard, or prevent, the formation of starvation induced stomach ulcers in rats.

The results show …

A Comparative Study Of Conventional Exercise And Weight Training Of College Women At Utah State University, Virginia Gayle Oleksiak

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Women are now making use of weight training for improving strength, flexibility and body contours. Most studies on weight training have used male subjects. This study compared the results of a conventional exercising program and a weight training program for reducing inches in the circumferences of the right upper arm, pectorals, waist, hips, and right thigh.

The subjects were twenty-nine female students in figure control classes at Utah State University. The control group of sixteen subjects exercised in the conventional manner, and the experimental group of thirteen subjects exercised on the weight training machine. Subjects were asked not to change …

An Evaluation Of Stress Induced Mortality Of Stocked Catchable-Sized Rainbow Trout In Temple Fork Of The Logan River, Robert Earl Gresswell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The level of stress imposed by population pressure, handling and live transportation on planted catchable-sized rainbow trout in a northern Utah stream was examined. Production of adrenocorticotrophin, as measured by interrenal ascorbic acid and serum cortisol levels, did not occur in transported or planted fish.

Dead or moribund fish collected from stream or live boxes comprised 13 percent of the 2,000 fish planted. Aeromonas salmonicida, the causative agent of furunculosis, was isolated from 41 percent of 106 moribund fish sampled. In addition, 39 percent of the samples exhibited bacterial growth other than A. salmonicida. Apparently, handling, transportation, and planting did …

Immunological Response Of Rats Fed Decreasing Levels Of Dietary Iron And Calcium With Or Without Vitamin D, Bernice Nell Neibaur

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A considerable amount of research has shown that a severe dietary deficiency of protein or vitamins will markedly depress antibody synthesis. Very little work has been done with the effect of mineral deficiencies on antibody production, and no evidence has been presented showing the relationship of the effects of small changes in nutrient intake to the sensitivity of the antibody response to small degrees of malnutrition and the relationship of dietary iron, calcium and vitamin D to the production of antibodies.

In two experiments, it was shown that as the level of iron in the diet is decreased, the production …

Serum Lipids And Urinary Estrogens Of Non-Pregnant Menstruating Young Women, Shiao-Fan Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twelve university women students served as experimental subjects in a study of the serum lipids and urinary estrogens of healthy nonpregnant menstruating young women, who were living under their usual conditions.

The subjects maintained constant weight on their ordinary diets during the entire study period. Antecubital blood and 24-hour urine specimens were collected on certain days which represented different stages of the menstrual cycle. Quantitative analyses were made on serum total cholesterol, lipid phosphorus (phospholipids), triglycerides and total lipids. Gas-liquid chromatographic analysis of the fatty acid composition of each serum lipid component was also made. Urinary estrone, 17β- estradiol and …

Laboratory Studies Of Two Species Of Jackrabbit Bot Flies, Craig R. Baird

Biology

The life history and host and site specificity of Cuterebra jellisoni Curran and Cuterebra ruficrus Austen were studied under laboratory conditions. Adult flies of each species were induced to mate. C. jellisoni eggs required seven to eight days for incubation, and C. ruficrus required nine to ten days. First instar larvae were successfully introduced into lagomorph hosts via all natural openings. Regardless of the introduction site, however, each species of larva was later found in a specific terminal site. C. jellisoni larvae completed development in jackrabbit hosts in 36 days and were found in subcutaneous cysts on the head. C. …

Nutrition In Thailand, Methee Larptavee

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Thailand is located in souteast Asia between Burman, Laos, and Cambodia. It has a long coast line as one strip of land reaches out into the sea. It has a warm climate with much rain, especially in the south. There are 27 million people in the area of 200,000 square miles. Rice ranks number one in the Thailand diet. Next to rice is fish, both from fresh water and from the sea.

A nutritional survey was first made in Thailand in Bang Chan Village in 1952-54, in connection with the Cornell-Thailand project. Since the results covered only a small segment …

Studies Related To The Control Of Anacamptodes Clivinaria (Guenée) (Lepidoptera: Geometridae), Harold W. Flake

Biology

Economic importance Anacomptodes clivinaria (Genee) is a serious pest of curlleaf mountain mahogany (Cercocarpus ledifolius) and bitterbrush (Prushia tridentata). Mountain mahogany and bitterbrush are important browse plants for wildlife and for watershed protection. In the winter, curlleaf mountain mahogany may serve as the major food source for wildlife because the leaves are retained year around (Flook, 1955). The deer and antelope winter ranges are some of the limiting factors in herd size and must be protected. Smith (1957) reported that curlleaf mountain mahogany has one of the highest percent nutrients of any winter browse plant for …

Studies Related To The Control Of Anacamptodes Clivinaria (Guenée) (Lepidoptera: Geometridae), Harold W. Flake

Biology

Economic importance Anacomptodes clivinaria (Genee) is a serious pest of curlleaf mountain mahogany (Cercocarpus ledifolius) and bitterbrush (Prushia tridentata). Mountain mahogany and bitterbrush are important browse plants for wildlife and for watershed protection. In the winter, curlleaf mountain mahogany may serve as the major food source for wildlife because the leaves are retained year around (Flook, 1955). The deer and antelope winter ranges are some of the limiting factors in herd size and must be protected. Smith (1957) reported that curlleaf mountain mahogany has one of the highest percent nutrients of any winter browse plant for …

Studies Related To The Control Of Anacamptodes Clivinaria (Guenée) (Lepidoptera: Geometridae), Harold W. Flake

Biology

Economic importance Anacomptodes clivinaria (Genee) is a serious pest of curlleaf mountain mahogany (Cercocarpus ledifolius) and bitterbrush (Prushia tridentata). Mountain mahogany and bitterbrush are important browse plants for wildlife and for watershed protection. In the winter, curlleaf mountain mahogany may serve as the major food source for wildlife because the leaves are retained year around (Flook, 1955). The deer and antelope winter ranges are some of the limiting factors in herd size and must be protected. Smith (1957) reported that curlleaf mountain mahogany has one of the highest percent nutrients of any winter browse plant for …

The Secret Of Viral Reproduction, George W. Cochran

Faculty Honor Lectures

Nucleic acid is the basis of life. There are 2 types, ribose nucleic acid (RNA) and deoxyribose nucleic acid (DNA). DNA, the material found in the chromosomes within the nuclei of the cells of most living organisms, bears the genetic code that determines all of the characteristics of a living organism. Information from the DNA of the chromosomes is carried to all parts of the cell by one type of RNA called messenger RNA. Additional specialized RNA molecules are found inside the ribosomes, tiny bodies within the protoplasm that make proteins for the cell. Still other, smaller RNA molecules, called …

The Effect Of Nutritional Education On The Food Habits Of High School Students, Gladys C. Hyer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

"From the day that Adam and Eve shared an apple in the Garden of Eden, food and the partaking of food have been of infinite significance in the life of man."

There is a growing concern among nutritionists and others about the food habits of teenagers, Although the United States is considered to be the best fed nation in the world, there is an important group of our population, abut 16,000,000 teenagers, who have alarmingly poor food habits.

Immunology Of Coccidiosis Caused By Eimeria Bovis In Calves, Clyde Merle Senger

Biology

Coccidiosis is a disease which can occur in most vertebrates and certain invertebrates as a result of infection by organism classified in the Order Coccidia of the Class Sporozoa, Phylum Protozoa. Host animals become infected by ingesting the exogenous resistant stage, the oocyst (Figures 1 and 2). These oocysts may be ingested with contaminated food and water or from the bodies of other animals. In the intestine of the new host, the oocysts rupture and release a specific number of sporosoites depending upon ths genus of coccidia involved. The liberated sporesoites penetrate epithelial or other cells of the intestine and …