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Production And Characterization Of Monoclonal Antibody And Its Comparison To Polyclonal Antibody For Detection Of Virus, Bruce G. Harper

Biology

Monoclonal antibodies used in serological procedures have the potential to recognize and identify one single antigenic determinant on a virus that is not shared by closely related viruses. This procedure would have the advantage of reducing non-specific background and would allow differentiation between a mixture of viruses. In this investigation, a monoclonal antibody to reovirus type 1 was produced and compared with polyclonal antibody for immunoassay detection of virus. The tests compared were the enzyme-linked immunosorbent assay (ELISA), the enzyme-linked fluorescent assay (ELFA), and the immunofluorescent cell count assay. A comparison of monoclonal antibody to polyclonal antibody in an immunofluorescent …

In Vivo Development Of Acosphaera Aggregata Skou In The Alfalfa Leafcutting Bee, Megachile Rotundata(Fabricus): A Light And Electron Microscope Study, William R. Mcmanus

Biology

The present light and electron microscope study revealed that the in vivo pattern of development of A. aggregata resembles that of the in vitro development of A. apis, the only other member of the Ascospheraceae to be studied in detail by Spiltoir in 1955. As well as documenting the similarities between the two species, this study also revealed several distinct differences between the two species and answered several developmental questions left unanswered by Spiltoir's study. Nongerminated and germinating ascospores were seen within the gut lumen of the feeding larvae. After germination minimal hyphal development occurred within the gut lumen. The …

Murine Rotavirus Infection: Characterization Of The Disease And Influence Of Host Malnutrition And Dietary Protein, Roger Lee Noble

Biology

A possible correlation between malnutrition and the increased incidence and severity of rotavirus-associated gastroenteritis in human infants was investigated using a mouse model originally described by others, but which was further defined and characterized as . a part of these studies. Two- or three-day-old specific-pathogen free Swiss Webster mice were orally infected with a 10% homogenate of intestines from mice infected with murine rotavirus. In the resulting disease, gross signs (diarrhea, alteration in intestinal appearance, failure to gain weight) and histologic changes typical of rotaviral gastroenteritis were manifest. Although quantification was not feasible by immunofluorescent staining, this technique did reveal …

The Effects Of The Antifertility Agent Af1312/Ts On The Testis And The Epididymus Of The Laboratory Mouse: A Light And Electron Microscope Study, Lyla M. Wagley

Biology

AF1312/TS, an antifertility agent synthesized by Silvestrini and associates in Italy, has the potential to be developed as a contraceptive in human males. This chemical's action is extremely specific, affecting initially, only the elements of the seminiferous epithelium. The drug's action is reversible, and dose, and species specific. This study was undertaken to observe the general morphplogical changes in the testis and epididymis of the mouse due to oral dosing with AF1312/TS. Damage was so drastic, it was evident with the light microscope. Fine structural analysis was used to determine the cell types involved, as well as the organelles damaged …

The Effects Of Neutral And Sex-Specific Terminology On Sex Stereotyping, Natalie J. Malovich

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study was conducted to examine the effects of neutral and sex-specific terminology on sex stereotyping in regard to two primary questions: 1) whether or not the use of sex-neutral terminology alters subjects ' associations to particular words, and 2) whether, in the absence of gender identification, subjects make traditional sex-role assumptions about neutral terms. A third questions examined potential differences in male and female subjects' responses to neutral and sex-specific terminology.

Using a semantic differential technique, 40 male and 40 female volunteer subjects described a number of occupations and roles identified by sex-specific or sex-unspecified labels. Descriptions of those …

Mother-Infant, Father-Infant Relationships, Soheila Sobhani

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was designed to determine the contributions of mothers and fathers to infant social development. Nine 10-month-olds, 12 fourteen-month-olds, and 12 eighteen-month-olds were observed with their mothers and fathers in a laboratory situation. Parent-infant interactions were videotaped during three different episodes: Mother-infant dyad, father-infant dyad, and mother-father-infant triad. Findings revealed different interaction patterns as a result of the ages of infants and the interaction situation. Older infants and their parents engaged in more verbal behavior (responsive talk, social speech, and story reading) than younger infants and their parents. It was found that parents and infants interacted with each other …

Childhood Experiences In Mormon Polygamous Families At The Turn Of The Century, Dorothy Geneve Young Willey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The primary purpose of this research was to gain insight into what childhood was like in turn-of-the-century Mormon polygamous families. This purpose was executed through two main avenues: basic empirical data and descriptive accounts. This type of research was crucial inasmuch as previous research and commentaries dealt with adult relations but little was known about children in this complex Mormon family structure.

In order to gain an understanding of childhood in Mormon polygamous families during this era, forty elderly individuals who were reared in plural marriages were interviewed in depth. A field type design was employed using a historical-cultural; in …

Limitations Of Computerized Impact Assessment Models For Planning In Small Towns, Mark Richard Brown

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Western boomtowns have been the focus of intense planning activity since the early 1970's. The goal of this activity has been to manage rapid growth caused by large energy, industrial, and resource developments. Planning assistance has been made available to impact communities in a variety of forms including financial aid from senior levels of government and from impacting industries, economic and demographic forecasting models, growth-management consulting firms, case studies, and comprehensive handbooks for community action. There has been rapid mobilization of sophisticated administrative and technical expertise to mitigate the problems of impacted communities.

Nonpoint Source Pollution To Bear Lake, Utah-Idaho: Magnitude, Periodicity, And Watershed Management Alternatives, Victor J. Kollock

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

From April of 1981 to June of 1982 a Clean Lakes Program Phase 1 Diagnostic Feasibility Study was conducted on Bear Lake, a 282 km² (109m²) fresh-water lake located in southeastern Idaho and northeastern Utah. A portion of this Clean Lakes Program required an itemized inventory of all nonpoint sources affecting the lake's water quality.

A 16 month lake tributary monitoring program, which included the spring runoff periods of 1981 and 1982, provided data from which nutrient loading values (kg/day and kg/yr) were calculated. Analysis of these data, along with land use percentages within the Bear Lake …

Investigation Of Selected Aspects Of Kokanee (Onchorhynchus Nerka) Ecology In Porcupine Reservoir, Utah, With Management Implications, Paul Joel Janssen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several aspects of kokanee (Onchorhynchus nerka) ecology were studied in the fluctuating, 80ha Porcupine Reservoir, Utah in order to determine optimum management strategies. In 1981, escapement was enumerated and estimates of egg deposition were made. An estimate of the resulting fry recruitment was also attempted and was successful up to the arrival of spring runoff. In 1982, escapement was enumerated and estimates of egg deposition were made, with subsequent estimates of egg deposition and percent survival to the advanced eyed stage. Age and growth rates were determined and survival rates estimated.

Escapement for 1981 and 1982 was 5,463 …

Using Videodisc Technology And The Index Of Nutritional Quality To Teach Dietary Guidance To Young Adults, Glenda Wigginton Edmunds

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A review of the literature indicated a need for nutrition education materials to be current with prevailing scientific knowledge, using advanced instructional technology. Individuals need help in applying nutrition principles to their dietary practices.

A nutrition education program utilizing the nutrient density concept and the dietary guidelines for use on videodisc with microcomputer interface was developed and evaluated. The evaluation of the program did not prove to be effective statistically but learners did show an interest in the program and had positive feedback in regard to the information presented and the technology used.

The Effect Of Heat Treatment On Meat Heme And Nonheme Iron Bioavailability For The Anemic Rat, Oranong Jansuittivechakul

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three separate experiments were conducted to investigate the effects of heat treatment on meat heme and nonheme iron bioavailability in anemic rats. Male weanling Sprague-Drawley rats were first made anemic, then subsequently fed with experimental diets. In the first experiment, dietary iron sources were raw or autoclaved meat mixed with ferrous sulfate to give the ratios of iron from meat and ferrous sulfate equal to 100:0, 75:25, 50:50, 25:75, and 0:100 respectively. For the second experiment, the dietary iron sources were raw or autoclaved meat mixed with bovine hemoglobin (HB) to give dietary iron ratios as described for experiment 1. …

Efficiency Of Converting Iron Into Hemoglobin As An Assay For Evaluating Iron Bioavailability, Paul Whittaker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of iron absorption on subsequent hemoglobin regeneration was investigated by repletion and subsequent analysis of an anemic weanling rat model. Primary assay procedure in these studies was the Regeneration Efficiency method.

As repletion or curative assay techniques form the central body of investigative research in iron utilization, the first subject of inquiry was the effect of repletion interval and degree of depletion upon the animals as monitored by physical indicators such as blood volume, growth and hematinic response. Percent blood volume is a particularly useful figure in the calculation of the amount of hemoglobin iron but was found …

Development, Implementation And Evaluation Of An Education Course For The Utah State University Coordinated Undergraduate Medical Dietetics Program, Cynthia Olson Obermiller

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The entry-level clinical dietitian must possess competencies in the area of communications and in the process of education. Training and practice in these areas, as related to the profession of dietetics, must be provided during the professional phase of the dietetic student's training. The purpose of this study was to develop, implement and evaluate a course for the Coordinated Undergraduate Medical Dietetics Program at Utah State University. This course, entitled Education in Clinical Dietetics, was added to the junior year fall quarter curriculum. The ten-week course combined didactic and lab experiences to provide the students with a thorough background in …

Longitudinal Pantothenic Acid Status Of Pregnant And Lactating Women, Won Oack Song

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pantothenic acid nutritional status was evaluated in a cohort of twenty-nine Caucasian pregnant women, aged 20-35, during their third trimester of pregnancy, at two-weeks postpartum and at three months postpartum. Seventeen non-pregnant, non-lactating women who had similar demographic backgrounds as the pregnant women were selected as a control group and followed the same experimental schedule as the pregnant women. A fasting blood sample, two days 24-h urine specimens and diet record were obtained for each of two days from each subject at each period.

The mean dietary pantothenic acid intakes of the women during pregnancy (5.30±1.74 mg/day) and during lactation …

Meltability And Rheology Of Model Process Cheese Containing Acid And Rennet Casein, Paul Alexander Savello

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Process cheese models were prepared by blending acid or rennet casein, milk fat, sodium chloride, 2.5% emulsifying salt and water and heating to 80 C. Acid casein cheese models were subjected to sodium hydroxide conditioning at 65 C in the cooker. Model process cheeses were acidified with lactic acid and treated by addition of undenatured and heat-denatured whey protein, four different emulsifying salts and sodium oxalate.

Meltability and toughness of the model cheese increased to a maximum with increased sodium hydroxide conditioning of acid casein to pH 7.20. These same properties decreased with addition of undenatured and heat-denatured whey protein …

Natural Killer Activity In Gardner's Syndrome, Ann Marie Stembridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Gardner's syndrome is an autosomal dominant disease presenting multiple colonic polyps with a predisposition for malignant change. In addition to colonic polyp formation by early adolescence, extracolonic lesions appear often prior to polyp formation. One theoretical mechanism for the origin of polyps and malignancies in Gardner's syndrome is a genetic defect in the natural killer cell activity of patients with this disease. Natural killer cells are a subpopulation of lymphocytes that spontaneously lyse tumor cells and virally transformed cells. A study was undertaken to determine the natural killer activity of patients with Gardner's syndrome.

A technique termed chromium release was …

Predictors Of Corporate Funding For Environmental And Recreational Programs, Joel H. Staub

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this research was to develop and test a group of predictors that could be used by fundraisers to determine potential corporate support for environmental and recreational programs. Local surveys were conducted to determine environmental and recreational needs for Cache Valley and twenty interviews were held with local corporate managers. The information gathered in these interviews and the literature review provided a list of approximately 20 variables which were narrowed down to 10 to predict both the potential for giving and the level of giving.

A proposal for a youth training project was developed and local Forest Service …

Jacobsonian Versus Autogenic Relaxation Training: Interactions With Locus Of Control, Mark J. Weaver

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to evaluate the thesis that matching locus of control with type of relaxation training enhances physiological self-control. This was accomplished by comparing the effectiveness of a somatic and a cognitive relaxation method for internal and external locus of control subjects. It was hypothesized that the two techniques vary in degree of cue salience, and that the attentional strategy of internals would interact with the cognitive approach while externals would respond better to the somatic approach.

Subjects were 80 volunteers from stressful occupations who scored high on the State-Trait Anxiety Inventory A-trait scale. Forty internals …

On The Effectiveness Of Agricultural Land-Use Controls, Eric Tarquin Marnell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Land resources are a valuable resource in the economic system. There exists considerable controversy surrounding the allocation of land into the competing land uses. At the heart of this controversy are agricultural land resources. The purpose of this paper is to investigate, first theoretically and second empirically, the effectiveness of controls placed on land use to keep land in agricultural production.

A theoretical conclusion is reached as to whether the free market or 1 and-use controls allocate land resources more efficiently. An empirical model is formulated to explain changes in the quantity of agricultural land as a function of several …

A Dynamic Optimization Model Of Energy Related Economic Planning And Development For The Navajo Nation, Hamid Beladi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Navajo reservation located in portions of Arizona, New Mexico and Utah is rich in low sulfur coal deposits, ideal for strip mining operation. Presently, the Navajo Nation has entered into extensive coal leases with several large companies and utilities. Contracts have committed huge quantities of Navajo coal for mining.

The present research has been directed to evaluate the shadow prices of Navajo coal and identify optimal coal extraction. An economic model of coal resource extraction over time has been structured within an optimal control problem has been formulated as a discrete dynamic optimization problem.

A comparison of the shadow …

An Extension Teaching Demonstration Using Broiler Chicks As A Model, Joseph B. Ishaya

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This test demonstration was intended to develop a modern management program to improve broiler production in Nigeria through extension teaching methodology. The management programs included animal health, nutrition, and general care.

Some of the factors that limit broiler production in Nigeria are associated with the management strategy presently adopted by Nigerian subsistence farmers.

This report considers the effect of different protein sources on broiler nutrition and the techniques necessary to get the farmers to practice the result of up-to-date research findings.

The extension teaching demonstration reported here is based on 60 day-old chicks. Chicks were randomly assigned to two pens, …

Effects Of Physical And Emotional Stress, Catecholamines And Naloxone On Hdl And Ldl Cholesterol Levels In Rats And Man, Andrew G. Goliszek

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A series of investigations were undertaken to determine whether physical or emotional stress, catecholamines or naloxone (B-endorphon blocker) would increase serum total cholesterol and LDL and HDL levels. Physical stress given daily over a period of time caused a steady increase in serum total cholesterol and LDL without a significantly altering high density lipoproteins (HDL) or serum triglycerides. Daily injections of epinerphrine in oil caused an increase in both serum total cholesterol and LDL levels while daily injections of norepinephrine did not. Reversal of the treatments caused a reversed response in both groups of rats. Similar increases in both total …

Mule Deer Reproduction And Survival In The Lasal Mountains, Utah, Randall B. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mule deer (Odocoileus hemionus) reproduction and survival on the LaSal Mountains, Utah were studied from 1978-81. Reproductive rates were high, suggesting that summer and fall forage quality was adequate and was not responsible for low recruitment observed in recent years. The combined fetal rate, determined from laparotomies and carcasses, was 1.72 for all females older than 1 year (N = 114). Fetal rates were 1.15 for yearlings (N = 20), 1.80 for prime females (2-7 years, N = 78), and 1.75 for old females (8+ years, N = 16). No evidence of breeding was found among fawns (N …

Studies In Peptide Synthesis Towards The Lanthionine Analog Of Des-N-Tetramethyl Triostin A, Ganesh Devdas Kini

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Studies have been carried out towards the synthesis of bicyclic octadidepsipeptide lanthionine 4, an analog of des-N-tetramethyl triostin A. Starting with a symmetrical lanthionine unit 5 (R₁, R₃ = Me; R₂, R₄ = t-butyloxycarbonyl), synthesized from the corresponding cystine by contraction with hexaethylphosphorus triamide, linear octadidepsipeptides 29 and 32 were synthesized.

Attempted cyclizations of 29 and 32 to the bicyclic octapeptide were unsuccessful.

The failure of the symmetrical approach to 4 prompted the synthesis of an unsymmetrical lanthionine unit with appropriate compatible protecting groups. Attempts to synthesize unsymmetrical lanthionines by nucleophilic displacement on …

Accidental Releases Of Sour Gas From Wells And Collection Pipelines In The Overthrust Belt: Calculating And Assessing Potential Health And Environmental Risks, D. W. Layton, R. T. Cederwall, Y. E. Ricker, J. H. Shinn, K. D. O'Banion

All U.S. Government Documents (Utah Regional Depository)

Parts of the Overthrust Belt of western Wyoming and adjoining areas in Utah and Idaho contain geologic formations with significant accumulations of oil and natural gas. Some of these formations, though, yield gas that is contaminated with toxic hydrogen sulfide. As a consequence, the development of these so-called sour-gas reservoirs requires special safety procedures and technologies in order to prevent accidental releases of gas to the atmosphere that could cause adverse occupational and public health effects. To improve the analysis and assessment of wells and collection pipelines completed on lands leased from the Federal Government, the Minerals Management Service, Onshore …

Crsp Power Peaking Capacity: Generation At Outlet Glen Canyon Unit, Arizona: Concluding Report, Robert N. Broadbent, Clifford I. Barrett

Elusive Documents

No abstract provided.

Interagency Hazard Mitigation: 90-Day Post Flood Recovery Progress Report, Hazard Mitigation Team (Utah)

Elusive Documents

As a result of an extended period of abnormally high precipitation, prolonged snow melt, and subsequent high water tales, Utahns, in 1983 suffered from landslides, debris flows, flooding, and a dam failure. On April 30, 1983, the President determined the Spanish Fork slide-dam and Thistle flood covering Utah, Carbon, and Emery Counties was of sufficient seventy and magnitude to issue a Major Disaster Declaration under Public Law 93-288. The incident period was not closed until July 1, 1983. By the time all flooding had ceased, nineteen additional counties (over 75% of Utah) had been included in the Declaration.

Histological Differentiation Among Abiotic Causes Of Conifer Needle Necrosis, United States Department Of Agriculture, Forest Service

Forestry

Symptoms induced by phytotoxic gases within conifer needles can be differentiated histologically from those caused by other abiotic agents including winter drying, drought, and salt. However, it is not possible to differentiate among symptoms caused by hydrogen fluoride, sulfur dioxide, ethyl mercaptan, and hydrogen sulfide. Phytotoxic gases cause hypertrophy and hyperplasia of vascular parenchyma, endodermis collapse, and intense vascular staining. The other abiotic agents induce mesophyll collapse with little or no observable effects on vascular tissues. Histological analyses should be useful in diagnosis of air pollution-induced injury and damage in coniferous forests.

Planning For Rural Human Services: The Western Energy-Impact Experience, United States Department Of Health And Human Services, Office Of Human Development Services

Fuel Sources

Beginning in the mid-1970's, the development of energy resources in the western United States created significant changes in the small, rural communities of this area. Many of the changes were the result of the rapid influx of population associated with industrial activities. It has long been recognized that communities subject to rapid growth have difficulty meeting the physical facility needs of their residents, such as housing, shopping and school facilities, and sewer and water services. In recent years, a growing recognition of the importance of the social effects of rapid community growth has also developed. These effects include the deterioration …