Digital Commons Network^™

Genetics And Applications Of Nisin Production In Lactococcus Lactis Subsp. Lactis And Conjugal Exchange Of This Trait, Jeffery R. Broadbent

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Chapter I reviews current literature on gene transfer systems in lactic acid bacteria, how genetically altered microorganisms for food are presently regulated, and how nisin is used as a food preservative.

Chapter II investigates previous reports which linked genes for nisin biosynthesis and sucrose utilization (Nip⁺Suc⁺) to plasmid DNA in two well characterized L. lactis subsp. lactis strains. Plasmid curing studies, conjugations, and DNA-DNA hybridizations indicated that these genes were encoded by chromosomal loci in all Nip⁺Suc⁺ strains examined. Similar results were noted in nisin-sucrose transconjugants of L. lactis subsp. cremoris and S. …

Identifying Differences Among Male Sex Offenders: Child Molesters Versus Exhibitionists Versus Voyeurs, David P. Defrancesco

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Social histories and testing data were compiled during initial placement assessments of 147 individuals arrested and charged as child molesters, exhibitionists, and voyeurs. These data were statistically analyzed using discriminant analysis and analysis of variance (ANOVA). Child molesters, exhibitionists, and voyeurs were found to differ with regards to history of voyeurism; Minnesota Multiphasic Personality Inventory (MMPI) L scale scores; sexual arousal in response to a violent scenario involving a 12-year-old male, measured via penile plethysmography; and age. Implications of these results are discussed relative to the population sampled and future research.

The Effects Of Feeding A High Level Of Rumen Protected Fat With Rumen Undegradable Protein With Or Without Niacin On Rumen Fermentation Characteristics, Apparent Nutrient Digestibility, And Milk Production In The Early To Mid Lactation Holstein Cow, Carlos Eduardo Batallas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Forty high producing early to mid lactation Holstein cows were blocked according to stage of lactation and previous two-week milk yield (experiment 1). Eight ruminally and duodenally cannulated nonlactating Holstein cows were used for experiment 2. The objective was to determine the effects of the ration's high fat content (11.53%) when rumen degradable or rumen undegradable protein is fed with or without added niacin. Cows received one of five treatments: 1) basal ration (TMR); 2) basal ration with added rumen undegradable fat (RUF) (1.6 kg); soybean meal (SBM) (1.73 kg), and niacin (12 g); 3) same as treatment 2, without …

Influence Of 2,5-Hexanedione, Acrylamide, Tri-O-Tolyl Phosphate, Leptophos And Methylmercury On Endogenous Levels Of Tryptophan, Serotonin And 5-Hydroxyindoleacetic Acid And Serotonin Turnover Rates In Rat Brain, Craig H. Farr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several industrial and environmental chemicals cause distal and/or central neuropathy among other diverse toxic effects. Spague-Dawley derived rats were fed doses of 2,5-hexanedione, acrylamide, tri-o-tolyl phosphate, Leptophos and methylmercury via gavage. The dose levels and administration periods were established in previous experiments designed to assess clinical neuropathy using rats trained to walk on a rotorod apparatus fitted with an electrode floor. After intravenous injections of ³H-Tryptophan, whole rat brain homogenates were analyzed using liquid scintillation and spectrofluorometric techniques for levels of tryptophan, serotonin and 5-hydroxyindoleacetic acid. Serotonin turnover rates were calculated using the specific activities of tryptophan and serotonin …

The Efficacy Of Multidisciplinary Treatment Programs For Chronic Low-Back Pain: A Meta-Analysis, Jane E. Curtis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Chronic low-back pain is a prevalent and costly problem for many adults in the United States. Currently, multidisciplinary treatment approaches are the treatment of choice for this problem. A meta-analysis was conducted on 43 published studies to describe the nature of these programs, the patients involved in them, treatment efficacy at discharge and follow-up, and possible relationships between these characteristics and outcome.

Results show that these programs were often in university medical settings, with an emphasis on active patient participation. Common treatment approaches included physical therapy, skills training, medication management, supportive therapy, and behavior modification. Patients involved in these programs …

Effects Of Knowledge Of Accountability In Mastery Learning Programs On Academic Achievement, Goal Setting Characteristics, And Locus-Of-Control Orientation, Donn C. Ritchie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

During the past quarter century, over 1000 articles have documented changes in student behaviors related to participation in mastery learning programs. Although the results have been generally positive, a debate continues as to the cause for increased student performance: Are results due to changes in how students attend to the information, or simply due to increased study time as a result of remediation?

In this study, a videodisc-based program in fractions was used with fifth-grade students. The videodisc-based instruction was chosen to help minimize differences in instructional materials, instructional time, and instructional delivery. A pretest-posttest, control-group design was used to …

Optimization Of Ranch Management Alternatives In Utah, Scott G. Evans

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine the optimum combination of various cattle production, range forage, and crop harvesting alternatives available to ranch owners and managers in Utah. While many promising alternatives are available, determining which alternatives to implement is difficult because the total ranch operation must be considered.

Linear programming (LP) is a tool available to ranch managers which allows the profit maximizing combination of improvements to be easily determined. LP allows ranchers to examine the entire ranch operation and to reduce the amount of risk and uncertainty involved in the decision making process.

The typical Utah ranch …

Implications Of Mediating Parent-Child Interactions In The Treatment Of Child Abuse, Connie Faye Nelke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Child abuse has been shown to have a detrimental effect on the emotion development of the abused child. Due to a history with the parent that includes a high proportion of negative interactions and an impaired parent-child relationship, the child who has been subjected to physical abuse often manifests psychological symptoms. The purpose of this study was to investigate the effect that treatment aimed at increasing the frequency of positive interaction in the parent-child relationship had on the emotional status of the abused child. Results showed that parent-child interaction play sessions were effective in increasing positive interaction and decreasing subsequent …

The Influence Of Dietary Factors On The Risk Of Urinary Stone Formation, A. Hesse, R. Siener, H. Heynck, A. Jahnen

Scanning Microscopy

The action of various beverages and foods on the composition of the urine in the circadian rhythm and in the 24-hour urine has been investigated under standardized conditions. Orange juice leads to a significant increase of urinary pH and citric acid excretion. Black tea leads to a raised excretion of oxalic acid by only 7.9%. In the short term, beer increases diuresis, but afterwards leads to a compensatory antidiuresis with increased risk of stone formation. Depending on their composition, mineral waters have very different effects on the urinary constituents. Milk as well as cocoa beverage significantly increase calcium excretion; moreover, …

Relationship Between Villous Shape And Mural Structure In Neutron Irradiated Small Intestine, K. E. Carr, J. S. Mccullough, A. C. Nelson, S. P. Hume, S. Nunn, H. H. M. Kamel

Scanning Microscopy

Previous work on irradiation of mouse small intestine has assessed the changes produced by counting crypts/microcolonies, scoring villous shape or examining morphological changes in specific parts of the wall.

This paper used scanning and transmission electron microscopy to study the effects of whole body irradiation with 5 Gy neutrons on the surface and internal features of the intestinal wall of CFLP mice, 1 day, 3 days and 7 days after treatment.

Empirical scores from the ultrastructural findings were inserted into a Morphological Index display calculated from analytical data based on cell counts and area measurements obtained from resin histology sections. …

Assessing The Pre-School Classroom Environment For Facilitative Emergent Literacy Activities And Strategies, Maggie Harris

Undergraduate Honors Capstone Projects

In one of his educational goals for the nineties, President Bush declared that "By the year 2000, all children in America will start school ready to learn [i.e., in good health, having been read to and otherwise prepared by parents.]" (Elam, 1990, p. 41). By providing this particular goal, the President clearly emphasized the importance of the pre-school years for preparing children for school. Further, Elam interpreted this goal to mean that children must be prepared for school by their parents and that reading to children is one important factor in the child's readiness for learning.

Scanning Electron Microscopy And Energy-Dispersive X-Ray Microanalysis Studies Of Early Dental Calculus On Resin Plates Exposed To Human Oral Cavities, T. Kodaka, Y. Ohohara, K. Debari

Scanning Microscopy

Dental calculus formed after 10 days on resin plates, applied to the lingual sides of the mandibular gingival regions in eight human subjects, was investigated by means of scanning electron microscopy (SEM) and energy-dispersive X-ray microanalysis (EDX). The mineral deposits were mainly divided into three types: A, B, and C. The type A deposits showing an average Ca/P molar ratio of 1.42 were densely packed with fine needle-shaped crystals formed by the intra- and extracellular calcification. The type A deposits, probably composed of Ca-deficient apatites and the transitional forms between apatite and octacalcium phosphate (OCP), were observed in all subjects. …

Utah Science Vol. 53 No. 1, Spring 1992

Utah Science

3 Preparing Students for the 21st Century

11 Welcoming New Students and New Ideas

13 Research in Brief

23 Hands ...for a Hands-On Education

26 Concurrent Credit Enrollment in Agriculture: The Evidence is Mixed

29 Increasing the Department's Role in Research

Visualizing Cells In Three Dimensions Using Confocal Microscopy, Image Reconstruction And Isosurface Rendering: Application To Glial Cells In Mouse Central Nervous System, Frank Morgan, Elisa Barbarese, John H. Carson

Scanning Microscopy

This paper describes a general method for visualizing individual cells in intact tissue in three dimensions. The method involves immunostaining intact tissue to label specific cells, "optical sectioning" the stained tissue by laser scanning confocal microscopy, computationally reconstructing a three dimensional image data set from the digitized confocal optical sections, delineating isosurfaces of specific intensity within the reconstructed image by a "marching cubes" algorithm to generate polygon meshes defining boundaries of cells, and displaying individual cells, identified as three dimensional objects enclosed by contiguous polygon meshes, using computer graphics techniques. Each of the components of this method has been described …

Calculation Of Barriers To Proton Transfer Using Variations Of Multiconfiguration Self‐Consistent‐Field Methods. Ii. Configuration Interaction, K. Luth, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

Various means are tested of including additional electron correlation into multiconfiguration self‐consistent‐field (MCSCF) methods for computing proton transfer potentials in HF₂⁻, H₇N₂⁺, H₃O₂⁻, and H₅O₂⁺. Configuration interaction allowing single excitations (CIS) and configuration interaction with single + double excitations (CISD) calculations are performed following MCSCF expansion of the wave function using various different MCSCF reference wave functions. The CISD results are excellent, being fairly independent of choice of reference space although it is important that the occupied orbitals be balanced between the …

A Light, Transmission And Scanning Electron Microscope Study Of Snuff-Treated Hamster Cheek Pouch Epithelium, S. H. Ashrafi, A. Das, R. Worowongvasu, B. Mehdinejad, J. P. Waterhouse

Scanning Microscopy

The effects of smokeless tobacco (snuff) on hamster cheek mucosa were studied by light microscopy, transmission (TEM) and scanning electron microscopy (SEM). Two grams of commercially available smokeless tobacco were placed into the blind end of the right cheek pouch of each experimental animal, once a day and five days a week for 24 months. The control animals did not receive smokeless tobacco. After 24 months treatment with smokeless tobacco, hamster cheek mucosal epithelium lost its translucency and had become whitish in color. By light microscopy hyperorthokeratosis, prominent granular cell layers with increased keratohyalin granules and hyperplasia were seen. At …

Structural Artifacts And Advantages Of Cytocentrifugation Of Cells As Viewed By Scanning Electron Microscopy, Mário N. D. Peão, Artur P. Águas, Carlos Moreira De Sá, Nuno R. Grande

Scanning Microscopy

Cytocentrifugation of cell suspensions onto glass slides is a widely used procedure in contemporary cytology. We employed here scanning electron microscopy (SEM) to investigate putative morphological changes induced in cells submitted to cytocentrifugation. The fine structure of murine pleural exudate cells (macrophages mainly) processed by spinning was compared with that of similar cells treated without centrifugation (poly-L-lysine attachment of the cells to glass slides at 1 g). Cells of cytocentrifuged preparations showed a significant increase in diameter and smoothening of the cell surface as compared with the morphology of non-centrifuged cells. Cytocentrifugation also induced the formation of thin elongations coming …

The Effect Of Serial Passaging Of Lactobacilli In Liquid Medium On Their Physico-Chemical And Structural Surface Characteristics, Pieter L. Cuperus, Henny C. Van Der Mei, Gregor Reid, Andrew W. Bruce, Antoine E. Khoury, Paul G. Rouxhet, Henk J. Busscher

Cells and Materials

The aim of this study was to examine the effect of serial passaging in liquid medium on physico-chemical and structural surface characteristics of two lactobacillus strains. To this end, starting cultures (p = 1) of urogenital isolate L. acidophilus RC 14 and poultry isolate L. fermentum B54 were serially passaged in liquid medium up to 20 times (p = 20). In both strains, similar physico-chemical and structural changes were observed following serial passaging. According to water contact angle measurements , cells in p = 20 cultures became less hydrophobic. Microelectrophoresis showed that isoelectric points shifted towards the acidic region upon …

Dolores River Basin Water Quality Study, Department Of Interior; Bureau Of Reclamation, Technical Service Center; Denver, Co

Elusive Documents

No abstract provided.

1991-1992 Annual Report, Various Authors

Annual Reports

No abstract provided.

Economic Freasibility Of A Processing Facility For "Spent" Laying Hens In Northern Utah, Deevon Bailey, T. F. Glover, Chris Fawson, G. W. Squires, J. L. Park

Economic Research Institute Study Papers

No abstract provided.

Effects Of Fluoride On Precipitation And Hydrolysis Of Octacalcium Phosphate In An Experimental Model Simulating Enamel Mineralization During Amelogenesis, M. J. Mura-Galelli, H. Narusawa, T. Shimada, M. Iijima, T. Aoba

Cells and Materials

The present study was undertaken to investigate effects of fluoride at low concentrations on the precipitation of calcium phosphates on hydroxyapatite seeds in media resembling the enamel fluid composition. To this end, we used a new crystal growth model adopting a miniaturized reaction column. Experimental solutions had the composition similar to that found in the enamel fluid, except for calcium concentrations which were adjusted at 1.0 and 0.5 mM. Precipitation reactions taking place in the system at the higher supersaturation ([Ca] = 1 mM) were characterized by co-precipitation of platelike OCP and rod-like apatite crystals, while the reaction at the …

Molecular Strategies To Improve Protein Quality And Reduce Flatulence In Legumes: A Review, Benito O. De Lumen

Food Structure

The application of recombinant DNA and related techniques to plants opened up the potential to improve agronomic characters. food processing traits and food quality properties of plants as food sources. This review focuses on molecular approaches to improving the protein quality and reducing flatulence in beans. Much is now known about the regulation of seed-specific expression and organization of seed storage protein genes. Several attempts have been made to increase the methionine content of tobacco seeds using heterologous seed protein genes from peas, common bean and soybean. Such techniques could also be used to modify lysine, tryptophan and threonine contents …

The Binding Of Urinary Components And Uropathogens To A Silicone Latex Urethral Catheter, G. Reid, C. Tieszer, R. Foerch, H. J. Busscher, A. E. Khoury, H. C. Van Der Mei

Cells and Materials

The adhesion of uropathogens to urethral catheters initiates the infectious process which causes morbidity in a large patient population. Catheters were examined by X-ray photoelectron spectroscopy after use in three patients and following incubation in vitro with human urine, urea and creatinine, and found to have adsorbed a conditioning film containing carbon, nitrogen and in some cases phosphorous, calcium and sodium containing components. The binding of three strains of uropathogenic bacteria to the catheter surface was significantly influenced by the suspending fluid composition.

The Role Of Implant Surface Geometry On Mineralization In Vivo And In Vitro; A Transmission And Scanning Electron Microscopic Study, B. Chehroudi, J. Ratkay, D. M. Brunette

Cells and Materials

The purpose of th.is study was to examine the effect of substratum surface topography on bone formation in vivo and in vitro. Precise control over substratum topography was achieved using micromachining, a technique developed from the fabrication of microelectronic components. In the in vivo studies, titanium-coated epoxy replicas of micromachined surfaces were implanted subcutaneously in the parietal area of rats. After 6 weeks, bone-like tissue was found adjacent to some micromachined surfaces. Detailed observation of this tissue with the transmission electron microscope revealed osteoblast/osteocyte-like cells and a fully or partially mineralized collagenous matrix. Mineralized matrix and collagen bundles were …

Fine Structure Of Remodeling Sites On Iliac Cancellous Bone In Senile Osteoporosis: A Study By Scanning Electron Microscopy Using An Improved Organic Specimen Preparation Method, Toshihiko Takita, Tomonori Naguro, Kichizo Yamamoto

Cells and Materials

We improved on the organic specimen preparation method in order to investigate the details of the functional state of bone in osteoporosis at high magnification level. The usefulness and possibilities of this method are discussed showing fine structural images of remodeling sites. Iliac bones of senile osteoporotic patients obtained by transilial biopsies were studied by scanning electron microscopy (SEM) using the improved preparation method. Excepting the region near the corticoendosteal surface, most of the iliac cancellous bone was composed of thin rod-shaped trabeculae. Remodeling sites on trabeculae appeared as concavities of various sizes and were easily distinguished from inert surfaces. …

Changes In Intracellular Ca2+ And Structure In Platelets Contacting Synthetic Substrates, L. M. Waples, O. E. Olorundare, S. L. Goodman, R. M. Albrecht

Cells and Materials

Platelet structural changes such as spreading and cytoskeletal reorganization that occur upon contact with synthetic surfaces have been well documented . Structural changes such as spreading will vary depending on the substrate involved . Although platelet structural changes following adhesion have been recorded , the cellular mechanisms including changes in intracellular calcium flux underlying these platelet responses are less well understood. In this study, video microscopy was used to image platelet adhesion and spreading while simultaneously imaging intracellular free Ca2+ levels in individual Fura-2 loaded human platelets. This was accomplished using fluorescence video microscopy coupled with video enhanced asymmetric illumination …

Effect Of Ionizing Y-Radiation On Thermoluminescence And Electron Spin Rosonance Intensities In Milk Protein Concentrate Powders, J. Kispeter, L. I. Horvath, L. I. Kiss

Food Structure

Milk protein concentrate powder has found a wide application as a food ingredient. We investigated the effects of ionizing ')'-radiation at doses ranging from 2 to 20 kGy on electron spin resonance and thermoluminescence intensities in samples of milk protein concentrate powder with varying protein contents (36-73 %wt), containing addltional Fe++ ions (12-910 ppm) and stored under different conditions.

Electron spin resonance and thermoluminescence intensities increased unambiguously with absorbed -ydose. Added Fe++ ions showed a quenching effect as measured by both methods. Storage conditions affected strongly the decay of electron spin resonance signal intensity . Lineshape analysis of thermoluminescence curves …

Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson

Food Structure

'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a significantly smaller decay index was observed on the apple slices submerged in papain solution than on the control slices. Under SEM, less severe cell wall breakdown was observed on the apple tissues treated with papain than on apple tissues without treatment. Less spores were also observed on the papain treated apple slices than apple slices without treatment. Apple tissues treated with papain solution and distilled water also demonstrated noticeable st ru ctural differences. The apple tissues treated with papain solution for 18 hours retained the original cell structure while the cells in the apple tissues treated with distilled water collapsed.

Clinical Applications Of Recombinant Gene Technology: Bone And Cartilage Repair, Thomas A. Einhorn

Cells and Materials

Over the past decade, the field of molecular biology has given rise to the development of the applied discipline of molecular medicine. Based on recent developments using recombinant gene technology, genetic mapping analysis and other investigational tools, the physician and surgeon is now ready to begin using those instruments in the diagnosis and treatment of musculoskeletal disease. This article reviews the history of scientific exploration in recombinant gene technology as it applies to bone and cartilage repair. Clinical cases are presented to show how the fruits of scientific knowledge may be brought to bear on some of the more challenging …