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Changes In The Esophageal Epithelium In Rabbits Treated By Cis-Dichlorodiammineplatinum As Studied By Electron Microscopy, M. Albertsson, C. -H. Hakansson, M. Palmegren

Scanning Microscopy

The esophageal mucosa of the rabbit was investigated after a single dose of 5 mg Cis-Dichlorodiammineplatinum (Cis-DDP). Specimens were taken for scanning electron microscopy, transmission electron microscopy, and light microscopy. Examination was performed daily for 20 consecutive days. A cytotoxic effect was observed already the first day after injection with an intracellular oedema. Thereafter the height of the esophageal epithelium and the basal cell layer steadily decreased to a minimum day 11. This parallels the damaged microridges and an increased cell loss as revealed by scanning electron microscopy. At the end of the observation period the esophageal mucosa had completely …

The Effect Of 10 And 20 Gy Single Dose Irradiation On The Esophageal Mucosa Of The Rabbit. An Electron Microscopic Study, C. H. Hakansson, M. Albertsson, M. Palmegren

Scanning Microscopy

The mucosa of rabbit esophagus was irradiated with single doses of 10 and 20 Gy respectively. Specimens were taken for scanning electron microscopy (SEM), and light microscopy investigations. Examinations were made 1-14 days after 10 Gy and 1-17 days after 20 Gy. Irradiation resulted in edema during the first days. The thickness of the epithelium was at its lowest value around day 5 after the administration of 10 Gy, and on days eight to ten after the administration of 20 Gy. Damage of the epithelium surface, as scored on SEM micrographs, was most pronounced on days eight to eleven after …

Strontium And Bromide As Tracers In X-Ray Microanalysis Of Biological Tissue, Joanna Wroblewski, Samuel Sagström, Helmy Mulders, Godfried M. Roomans

Scanning Microscopy

Since energy dispersive X-ray microanalysis cannot distinguish between isotopes of the same element, alternative methods have to be used to get information similar to that obtained in experiments with radioactive tracers. In the present study, strontium was used as a tracer for calcium, and bromide as a tracer for chloride. Rats were injected with strontium chloride in vivo, and the uptake of strontium in the acinar cells of the submandibular gland was studied. Eventually a Sr/Ca ratio of 0.3 was reached. In some animals, secretion of mucus had been elicited by stimulation with isoproterenol 4 h prior to injection …

Bone Lining Cells: Structure And Function, Scott C. Miller, Louis De Saint-Georges, Beth M. Bowman, Webster S. S. Jee

Scanning Microscopy

Bone lining cells (BLC's) cover inactive (nonremodeling) bone surfaces, particularly evident in the adult skeleton. BLC's are thinly extended over bone surfaces, have flat or slightly ovoid nuclei, connect to other BLC's via gap junctions, and send cell processes into surface canaliculi. BLC's can be induced to proliferate and differentiate into osteogenic cells and may represent a source of "determined" osteogenic precursors. BLC's and other cells of the endosteal tissues may be an integral part of the marrow stromal system and have important functions in hematopoiesis, perhaps by controlling the inductive microenvironment. Because activation of bone remodeling occurs on inactive …

Osteoclast Features Of Cells That Resorb Demineralized And Mineral-Containing Bone Implants In Rats, Cedo M. Bagi, Scott C. Miller

Scanning Microscopy

The comparative temporal tissue response to demineralized bone matrix powder (DBP) and devitalized mineral containing bone powder (MCP) implanted subcutaneously in rats was studied. The tissue response to implanted DBP followed the well described endochondral osteogenic pathway and included the appearance of osteoblasts and osteoclasts. On the other hand, implanted MCP resulted in the appearance of a large population of giant cells that resorbed the implants. At later times (3-4 weeks), most of the cells in the MCP implants appeared as typical foreign body giant cells with extensive membrane foldings, usually away from bone surface. Some cells did, however, have …

Structural And Compositional Changes In Aging Bone: Osteopenia In Lumbar Vertebrae Of Wistar Female Rats, M. Silbermann, M. Safadi, D. Schapira, I. Leichter, R. Steinberg

Scanning Microscopy

Changes in vertebral bone mineral content and density during aging were quantified in female Wistar rats. This study represents a longitudinal follow up utilizing single photon absorptiometry for the measurement of bone mineral content (BMC), quantitative computed tomography (OCT) for the measurement of bone mineral density (BMD), and image analysis histomorphometry for the measurement of trabecular bone volume (TBV) and bone cortical area (BCA). The above measurements were accompanied by biochemical assays of calcium concentrations in the respective bones. All aging animals experienced significant decreases in BMC, BMD, TBV, BCA and in the calcium content of their bones. The above …

Microprobe Analysis Of Element Distribution In Bovine Extracellular Matrices And Muscle, M. B. Engel, H. R. Catchpole

Scanning Microscopy

The concentrations of some essential elements, Na, K, P, S and Cl were determined by microprobe analysis in bovine extracellular matrices of cartilage, tendon and elastic tissue (ligamentum nuchae) and in muscle cells. The values for the different tissues were compared and related to the blood electrolyte concentrations. Among the connective tissues the highest Na and lowest Cl values were found for cartilage which bears a high negative charge. The lowest concentrations of these elements occurred in elastic tissue which is relatively non-polar. In the three extracellular matrices sodium levels exceeded potassium. In myofibers potassium was the major cation at …

Bacillus-Shaped Deposits Composed Of Hexahedrally Based Crystals In Human Dental Calculus, T. Kodaka, A. Hirayama, K. Miake, S. Higashi

Scanning Microscopy

In human supra- and subgingival calculus, bacillus-shaped deposits showing various rocky-pile forms composed of hexahedrally based crystals were observed by scanning electron microscopy. The crystal size measured approximately 0.1 - 1.5 μm. The electron probe microanalysis always detected calcium, phosphorous, and magnesium. Their molar ratios resembled those of magnesium-containing whitlockite and moreover the crystals also gave the electron diffraction pattern of whitlockite. The bacillus-shaped deposits happened to coexist with the intracellular calcifying microorganisms, furthermore, oral microorganisms partially replaced by the hexahedrally based crystals were found. The crystal deposits were never seen in the surface layers of calculus exposed to the …

Quantitative Measurement From Vascular Casts, Ralph G. Kratky, Christine M. Zeindler, Dorian K. C. Lo, Margot R. Roach

Scanning Microscopy

A review of quantitative measurements show casting materials shrink from 0.2 - 20% and have viscosities ranging from 1.4 - 100,000 centipoise. Blood vessels have highly variable mechanical properties. Some microvessels are very stiff having little change in dimensions with pressure. Larger vessels generally change diameter significantly but show highly variable changes in length with pressure. Perfusion fixation does not fix the dimensions of blood vessels. Dog carotid arteries well fixed with glutaraldehyde at physiologic dimensions retain ≈20% of their elastic recoil circumferentially and ≈30% longitudinally. We recommend vascular casting as a method of accurately measuring the vasculature if care …

Surface Heterogeneity Of Tumor Cells And Changes Upon Ionizing Radiation, Z. Somosy, Orsolya Csuka, Tamara Kubasova, J. Kovács, G. J. Köteles

Scanning Microscopy

Heterogeneous distribution of surface domains is a characteristic feature of the tumor cell surface and the distribution differs from that of normal cells. During the malignant transformation the heterogeneity may change or disappear. Cell lines with various metastasizing capacities show different distributions of membrane domains or other differences in membrane or surface organization. We have demonstrated that the amount and distribution of negatively charged sites of B 16 melanoma membranes changed upon ionizing radiation (X-ray, ⁶⁰Co-gamma). In the case of the P 388 lymphoma, however, only the amount of negatively charged sites change after irradiation, the distribution remains unaltered. …

Oxalate Crystallization In The Kidney In The Presence Of Hyperuricemia, Shinshi Noda, Kenichi Hayashi, Kousaku Eto

Scanning Microscopy

It has been a long time since uric acid was suggested to be a promoting factor in calcium oxalate stones, and a number of in vitro studies have been carried out on the relationship between uric acid or urate and calcium oxalate.

Concerning in vivo studies, urate or calcium oxalate stone-forming diets were given alone in most cases, and diets that induce formation of stones with different composition have not been given in combinations. We administered a low-concentration oxalemic diet, and a mixed diet containing oxalic acid and uric acid, and biochemically and histologically studied the effects of oxalate and …

The Physical State Of Potassium In The Human Lymphocyte: A Review, William Negendank

Scanning Microscopy

Studies of the effects of chemical potential, temperature, and metabolic perturbation on static ion contents, kinetics of the approach to equilibrium, and kinetics of ionic self-exchange in human lymphocytes are reviewed. The results contradict the classical concept of cell ion and water physiology, the membrane-osmotic, pump-leak theory, and are re-interpreted by an adsorption model of the cell. In this model, most of cell water exists in a physical state sufficiently ordered to reduce the partition function of dissolved ions, and most of cell potassium is associated with fixed charges on macromolecules. Competing adsorption of potassium and sodium is cooperative and …

Gastrointestinal Surface Changes: Interpretation Problems And Indexing Possibilities (A Review), C. J. Pfeiffer, O. Bulbena

Scanning Microscopy

The purpose of this review on state-of-the-art and new perspectives on the use of scanning electron microscopy (SEM) in gastrointestinal pathology is to discuss the possibility of developing an index for quantitatively grading mucosal epithelial injury. This topic is reviewed within the framework of ulcer indices previously developed for gross lesions, where analogous problems exist, and in relation to the transmission electron microscope staging of epithelial cell pathology. If such an index could be developed it would increase objectivity and standardization of data analysis from laboratory to laboratory, and would allow for quantitative and statistical analysis of morphometric data. It …

Morphology And Chemical Composition Of Dental Calculi Mainly Composed Of Whitlockite, Toshiro Sakae, Hirotsugu Yamamoto, Hiroyuki Mishima, Takashi Matsumoto, Yukishige Kozawa

Scanning Microscopy

The morphology and chemical composition of two subgingival calculus samples, which were composed of magnesium-whitlockite with a Mg/Ca molar ratio of approximately 0.1 as shown by X-ray powder diffraction, were analyzed semiquantitatively using energy-dispersive spectroscopy (EDS) combined with scanning electron microscopy (SEM). The Ca/P molar ratio values for 33 EDS-analyzed materials ranged from 1.24 to 2.03 with an average of 1.49, and the (Mg+Ca)/P molar ratio values ranged from 1.43 to 2.28 with an average of 1.63. The average Mg/Ca molar ratio was 0.10, a value very close to that obtained by X-ray diffraction analysis.

The EDS-analyzed materials were grouped …

Cation Nuclear Magnetic Resonance (Nmr). 7li- And 23na-Nmr Results Obtained With Human Erythrocytes, Jan F. M. Post

Scanning Microscopy

A short discussion of practical results and theoretical aspects of nuclear magnetic resonance (NMR) on intracellular monovalent cations is presented. ⁷Li- and ²³Na-NMR relaxation studies on human erythrocytes are described which indicate that lithium and sodium ions are essentially free inside the cell. However, there is a contribution to transverse relaxation originating from cations diffusing through electric field gradients imposed by the cytoskeleton, which is reflected in the NMR line shape. ³⁹K-NMR results obtained by other authors for intracellular potassium are compared with the ⁷Li- and ²³Na-NMR results presented here. The few ³⁹K-NMR results …

An Investigation Of Animal Damage Associated With Maple Syrup Production, J. Alan May, Dennis Slate

Wildlife Damage Management Conference

An increase in the use of plastic tubing systems to collect sap from sugar maples (Acer saccharum) has allowed syrup producers to boost production in recent years, but not without cost. Rodents gnawing on tubing, spouts, and fittings may cause damage in excess of $300,000 annually in Vermont, the largest maple producing state. Red squirrels (Tamiasciurus hudsonicus), gray squirrels (Sciurus carolinensis), and chipmunks (Tamias striatus) appear to be responsible for the majority of damage. Other species including flying squirrels (Glaucomvs sabrinus), white-footed mice (Peromvscus leucopus), porcupines (Erethizon …

Farmer Attitudes Toward Wild Turkeys In Southwestern Wisconsin, Scott R. Craven

Wildlife Damage Management Conference

The reintroduction of the Wild Turkey (Meleagris gallopavo) represents one of the great success stories for Wisconsin wildlife management. Human settlement, habitat loss, and perhaps disease, combined to eliminate once-common turkeys from Wisconsin by 1900. Periodic attempts to restore a viable turkey population were unsuccessful with the exception of a small flock at Meadow Valley-Necedah in central Wisconsin. However in 1976, the Wisconsin Department of Natural Resources (WDNR) began a new, well conceived cooperative project with the Missouri Department of Conservation. Under the agreement, Missouri received 3 wild Wisconsin ruffed grouse (Bonasa umbellus) in exchange for …

Population Dynamics And Management Of Deer In Wisconsin, Keith R. Mccaffery

Wildlife Damage Management Conference

Management of deer in Wisconsin is affected by a combination of biological and environmental factors. In the Northern Forest, winter severity dramatically affects annual survival and recruitment. However, deer density goals here are above "I" carrying capacity. Thus, minor errors in harvest management are in part compensated by herd responses. Deer in the farmland areas of the state are maintained at goals that for the most part are below "I" carrying capacity. Errors in harvest management are magnified in farmland because herd responses are not compensatory. Therefore, the harvest quotas for antlerless deer must be more precise in our farmland …

Black Bear Damage In Virginia, Michael R. Vaughan, Patrick F. Scanlon, Sue Evelyn P. Mersmann, Dennis D. Martin

Wildlife Damage Management Conference

We examined records of black bear damage in Virginia on Shenandoah National Park, (SNP) (1979-1988) and non-Park lands (1973-1988). Mean annual damage was $5,470 on non-Park lands and $1,217 on the Park. Corn and beehives accounted for 79 percent and personal property for 72 percent of the economic loss on non-Park and Park lands respectively. Young males were responsible for most damage on non-Park lands while adults of both sexes caused most damage on SNP. Over 70 percent of bear damage incidents in the state occurred either on the Park or on land immediately adjacent to the Park. Moving bears …

Farmland Habitat Use By Wild Turkeys In Wisconsin, R. G. Wright, R. N. Paisley, J. F. Kubisiak

Wildlife Damage Management Conference

Habitat use and food habits of wild turkeys (Meleagris gallopavo) were studied during summer 1988-89 in southwestern Wisconsin in order to address growing concerns that turkeys cause considerable crop damage. Intensive telemetric monitoring in 1988 suggested that turkeys used crop fields (corn-alfalfa-oats) at a low rate compared to forest types. Brooded hens used forest habitats less and field habitats more than broodless hens and gobblers. Brooded hens appeared to use forest and crop field habitats less and non-crop fields (pasture and idle) more than expected. Broodless hens and gobblers appeared to use forest types and non-crop fields more …

Controlling Roosting Starlings In Industrial Facilities By Baiting, Bernice U. Constantin, James F. Glahn

Wildlife Damage Management Conference

During the winters of 1987-88 and 1988-89 a study was conducted to evaluate the potential of DRC-1339 baiting for controlling roosting European Starlings (Sturnus vulgarus) at Tennessee Eastman Company's chemical manufacturing plant in Kingsport, Tennessee. In 1987-88 Starlicide CompleteR (1% DRC-1339 treated poultry pellets diluted 1:9 with untreated poultry pellets) was used in preroosting congregating areas adjacent to the roost. In 1988-89 Starlicide CompleteR and DRC-1339 treated bread were used in bait containers placed in the roosting structure. Although 90 lbs of Starlicide CompleteR was consumed during 1987-88, bait consumption was sporadic and no appreciable change in the …

Florida's Nuisance Alligator Control Program, Michael L. Jennings, Allan R. Woodward, Dennis N. David

Wildlife Damage Management Conference

The recovery of alligators from centuries of exploitation, coupled with a burgeoning human population in Florida has resulted in an increasing number of problem animals and alligator attacks. In response to this problem, the Florida Game and Fresh Water Fish Commission implemented a nuisance alligator control program in 1978. A mean of 2513 nuisance alligators per year was harvested during the period 1978 to 1988. The number of alligator complaints, nuisance alligators harvested, and alligator attacks increased significantly over the period (P=0.0003, P=0.0001, and P=0.04). The value of alligator meat remained stable at about $5.00/pound over the same period, while …

Economic Impact And Control Of Wading Birds At Arkanas Minnow Ponds, Michael D. Hoy, Jeffery W. Jones, Albert E. Bivings

Wildlife Damage Management Conference

Esophageal/stomach contents of 172 little blue herons (Egretta caerulea), great egrets (Casmerodius albus), snowy egrets (Egretta thula), and great blue herons (Ardea herodias) were analyzed to estimate their consumption of golden shiners. Mean and maximum number of golden shiners consumed/bird, as well as the mean total length of golden shiners consumed by each wading bird species was determined. Loss estimates varied by wading bird species and ranged from $0.10 - $1.12/bird/feeding. Loss estimates reflect the severity of problems with wading birds on minnow ponds and the need to reduce losses by use …

An Automatic Trigger For Bird Frightening Devices, Steve Price, John Adams

Wildlife Damage Management Conference

This triggering unit for animal frightening devices was developed because of an expressed need for a more effective method of breaking up starling and grackle roosts. The USDA, Animal Damage Control (ADC) office in Louisville, KY suggested that a device which detected the presence of the birds before activating and then emitted different sounds would be much more effective than single functioning devices on timers. Existing frightening devices have limited effectiveness because the birds become accustom to the repeated and periodic actuations of the same thing over and over again. In November of 1986, Mr. Bernice Constantin, District Supervisor for …

The Usda-Aphis-Adc Program In The United States, Bobby R. Acord

Wildlife Damage Management Conference

The ADC program was transferred to the U.S. Department of Agriculture, Animal and Plant Health Inspection Service in 1986. Shortly after the transfer, the Secretary of Agriculture established the National Animal Damage Control Advisory Committee (NADCAC). Current and future issues facing ADC are discussed in the context of NADCAC recommendations.

Epa Update On Vertebrate Pesticides, William W. Jacobs

Wildlife Damage Management Conference

The U.S. Environmental Protection Agency regulates pesticides under the authority of the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA). Vertebrate pesticides are subsumed under the heading "rodenticides" and, under FIFRA, are regulated similarly to other pesticides.

Ecologically Sensitive Management Options For Bats, Paul B. Robertson

Wildlife Damage Management Conference

Over the past decade, there has been a dramatic, positive shift in the public image of bats in the United States (Tuttle 1988a). This shift is particularly impressive in light of the inappropriate and poor public image that bats have suffered in most western nations in the last century. Over the past decade, a sizable segment of the U.S. public, as well as local, state and national officials, have been educated to the ecological and economic value of bats which results from their insectivory and plant pollination activities (Olkowski and Olkowski 1989, Tuttle 1988b). The fact that they pose a …

Wildlife Damage In Electric Substations In New York, Jody W. Enck

Wildlife Damage Management Conference

Wildlife managers have long been concerned with the damage wildlife can cause, especially to agricultural crops. However, one area which has received little research is the damage caused by wildlife to electric substations. Such research is needed because damage to electric substations increases operating costs of utilities and reduces reliability of service to customers.

Six member utilities of the Empire State Electric Energy Research Corporation (ESEERCO) were surveyed to identify classes of substations experiencing animal-caused faults (i.e., short circuits), and to determine the impacts of those faults. Records of more than 200 animal-caused faults occurring from 1970-88 were examined. The …

Zinc Phosphide Rodenticide Reduces Cotton Rat Populations In Florida Sugarcane, Nicholas R. Holler, David G. Decker

Wildlife Damage Management Conference

Rodents cause extensive damage to sugarcane (Saccharum spp. hybrids) in southern Florida (Samol 1972). Losses have been estimated as high as $235/ha (Lefebvre et al. 1978). Cotton rats (Sigmodon hispidus) and roof rats (Rattus rattus) are responsible for most of the damage (Holler et al. 1981). In-field treatment is required for effective reduction of rat populations because of the distribution and restricted movement patterns of rats within fields (Lefebvre et al.1985a). Zinc phosphide (2%) baits are the only rodenticide baits registered for in-field use in Florida sugarcane. A preliminary test of in-crop aerial application …

Use Of Donkeys To Guard Sheep And Goats In Texas, Murray T. Walton, C. Andy Feild

Wildlife Damage Management Conference

Though livestock guarding dogs have received considerable attention in recent years, other animals including donkeys (Equus asinus) are being used to protect sheep and goats from predation by coyotes (Canis latrans). In Texas many ranchers prefer donkeys due to low cost, relatively small maintenance requirements, and compatibility with other predator control methods. This paper describes husbandry practices for use of guard donkeys and relates rancher accounts of their effectiveness in protecting sheep and goats.