Digital Commons Network^™

Patient-Specific Lumped-Parameter Model For Quantifying Vessel-Specific Remodeling And Predicting Right Ventricular Function In Pulmonary Hypertension, Christopher G. Lechuga, Amirreza Kachabi, Mitchel J. Colebank, Claudia E. Korcarz, Farhan Raza

Faculty Publications

Purpose: Pulmonary hypertension (PH) is a heterogeneous disease with patient-specific variability and vessel-specific remodeling, which eventually lead to right ventricular (RV) failure. The gold standard for RV assessment—pressure–volume (PV) loop acquisition—is invasive and limited to specialized settings. This study aims to develop a patient-specific lumped-parameter model that quantifies vessel-specific remodeling and simulates RV PV loops across PH phenotypes using routine clinical data.

Methods: A lumped-parameter model was calibrated using right heart catheterization and echocardiography data. Model agreement was assessed by R2 values for pressure and flow goodness-of-fit, and model-derived hemodynamic metrics were comparedwith clinical values. A dimensionality reduction approach was …

Characterization Of Ru(Ii) Polypyridyl Photosensitizers Bound To Tio2 Supports Through A New Covalent Metal-Ester Bonding Motif, D. M. S. C. Dissanayake, Christopher T. Lebarron, Laura C. Maybach, Joseph J. Kuchta Iii, Seyyedamirhossein Hosseini, Aaron K. Vannucci

Faculty Publications

The development of photoelectrochemical (PEC) devices involves the attachment of molecular photosensitizers onto solid supports. Particularly significant to this work are PECs that rely on ruthenium-based photosensitizers on high band gap metal oxide (MOx) supports. In this study, we have explored a new ester binding motif (MOx-ester) to covalently attach a ruthenium polypyridyl photosensitizer to TiO₂. This new MOx-ester photoanode is compared to the traditional photoanodes that utilize acid/base binding groups to attach the molecular Ru sensitizers to TiO₂. The new MOx-ester binding motif is characterized by cyclic voltammetry UV-Vis, solid state ¹³C NMR, and …

Psychological Resilience In The Covid-19 Response: A Systematic Review For Future Public Health Preparedness, Atena Pasha, Abdul-Hanan Saani Inusah, Jannatun Nayem, Xiaoming Li, Shan Qiao

Faculty Publications

Objective: The COVID-19 pandemic has highlighted the importance of psychological resilience for well-being. While several reviews have addressed resilience in specific populations or contexts, few have synthesized global literature across diverse populations. This review examines psychological resilience across diverse populations, focusing on measurement, associated factors, and future public health preparedness strategies. Method: Guided by the PRISMA, a thorough search of PsycINFO, PubMed, ScienceDirect, and Web of Science was conducted in February 2024, including quantitative studies considering psychological resilience during the COVID-19 pandemic published in peer-reviewed English journals from 2020 to 2024, using search terms relevant to psychological resilience and COVID-19. …

Green Runs: Colorado Cannabis Sales During Ski Season, Joshua H. Hess, Isabellle Bassock

Faculty Publications

This study examines the impact of ski tourism on Colorado’s retail marijuana (RMJ) sales, leveraging county-level sales data and ski resort characteristics. Using a two-way fixed effects model, we analyze seasonal variations in RMJ sales across counties with differing ski resort acreages. Our findings indicate that ski resort counties experience significant increases in RMJ sales during peak ski months (December–March), with the most prominent effects observed in counties with the largest ski resorts. The largest ski resort county– Summit County– sees an estimated seasonal increase of $4.35 million in RMJ sales. We estimate the isolated extra-state ski tourism effects and …

“When And Where [We] Enter…”? Intersectional Ideologies And Experiences In African American Support For Lgbtq+ Communities, Monique L. Lyle, Todd Shaw

Faculty Publications

This article examines how intersectional ideologies and experiences of marginalization affect the extent to which African Americans support or oppose the marginalization of LGBTQ communities. We posit that awareness of the race-gender positionality of African American women, as well as the unique positionalities of other marginalized African American subgroups, is critical to understanding the conditions under which Black Americans embrace LGBTQ rights and communities. Using the Black respondent subsample of the 2016 Collaborative Multiracial Post-Election Study, we test the extent to which intersectional theories explain African American cross-group consciousness with LGBTQ persons and support for LGBTQ rights and communities. In …

Community Synchrony In Aquatic Macroinvertebrates Is Unrelated To Environmental Variability But Differs Among Functional Feeding Groups, Anthony J. Pignatelli, Tad Dallas

Faculty Publications

Environments are becoming increasingly more variable, as a function of climate change. As this occurs, species may be exposed to conditions outside their preferred range. Such variability in the environment can influence community abundance as individual species respond either similarly (synchronous dynamics) or differently (asynchronous dynamics) to each other. These fluctuations in abundances are important for understanding the impact of environmental variability on species temporal fluctuations in aquatic macroinvertebrates. This group of organisms is species-rich and highly sensitive to environmental fluctuations. We analyzed 18 stream macroinvertebrate communities sampled by the National Ecological Observatory Network between 2014 and2022 to understand how …

Effects Of Leucine Supplementation On Athletic Performance, Central Fatigue, And Serum Metabolism In Endurance Athletes: A Randomized Controlled Trial And Targeted Metabolomics Study, Tieying Li, Wei Xu, Jun Chen, Zhaobo Kan, Xuemei Sui, Zhiguang Zhao, Qirong Wang

Faculty Publications

Objectives: We aimed to investigate the effects of 6 weeks of leucine supplementation on athletic performance, central fatigue, and serum metabolome in endurance athletes, and to provide valuable insights into nutritional strategies for endurance athletes. Methods: Twenty cross-country skiers were recruited and randomized into 2 groups: the placebo (PLA) group and the leucine (LEU) group. Subjects were given leucine (8.5 g) + sucrose (14 g) or only sucrose (14 g) supplements twice each day from Monday to Saturday for 6 weeks. Test parameters include body composition, aerobic capacity, isokinetic muscle strength, blood biochemistry, and targeted metabolomics. Results: After intervention, compared …

In-Situ Eval: A Modular Framework For Custom And Real-Time Rag Benchmarking, Ritvik Garimella, Kaushik Roy, Chathurangi Shyalika, Amit Sheth

Publications

Retrieval-Augmented Generation (RAG) has become the standard approach for integrating domain knowledge into Large Language Models (LLMs). However, fair comparison of RAG pipelines remains difficult: data preparation is often ad hoc, subsampling methods are opaque, parameters vary across implementations, and evaluation is fragmented. We present In-Situ Eval, a unified and reproducible framework that operationalizes the full RAG pipeline with configurable subsampling strategies and both RAG-specific and generic evaluation metrics. The platform supports two execution modes: an offline Dataset mode for evaluating precomputed outputs, and a live Retrieval mode for benchmarking RAG variants with state-of-the-art LLMs. Users can flexibly select datasets, …

Virtual And Remote Cardiac Rehabilitation For Older Adults: Current Evidence And Implementation Strategies, Sarah E. Slone, Rita D'Aoust, Rhonda Smith Wright, Victoria A. Yandle, Kurt G. Barringhaus

Faculty Publications

Purpose of Review

To present and analyze recent developments in remote cardiac rehabilitation for older adults, review outcomes data regarding clinical effectiveness of these modalities, and discuss implementation strategies targeted at older adults.

Recent Findings

Data from recent studies enrolling older adults into remote cardiac rehabilitation programs have yielded mixed results with some showing encouraging improvements in functional capacity and quality of life comparable to those obtained with center-based rehabilitation programs. Studies that showed no improvement in outcomes identified decreased patient engagement as an important driver of results. This underscores the importance of adapting remote cardiac rehabilitation programs to the …

Recalibrating The Risk Of False Confession Wrongful Convictions: Interrogation Tactics And Inverse Probability, Scott M. Mourtgos, Ian T. Adams

Faculty Publications

False confession wrongful convictions (FCWCs) are a serious failure of the criminal justice system. Although scholars have identified interrogation tactics thought to elevate this risk, existing research rarely estimates the population-level probability that legally permissible methods will produce an FCWC. Instead, inference relies on outcome-selected case series and laboratory diagnosticity ratios that ignore base rates and the far larger universe of interrogations without false confessions. This article offers a methodological recalibration. We formalize the outcome-selection problem and apply inverse probability logic to derive posterior FCWC risk integrating base rates, sensitivity, and specificity. Using Monte Carlo simulation, we synthesize available empirical …

Boiling A Frog: Pre-Adaptive Processes And Prison Riot Dynamics, Hayden P. Smith

Faculty Publications

This study explores the adaptive mechanisms and survival strategies deployed by incarcerated individuals during the Lee Prison Riot of April 15, 2018. Drawing solely on in-depth interviews with 25 incarcerated men (predominantly Black, aged 25–61) at Lee Correctional Institution, and utilizing the importation and deprivation models as analytical frameworks, the analysis reveals that early-life trauma, neighborhood disruption, and the construction of survival pride significantly shape inmate adaptation. In particular, the research documents how inmates are channeled into “high‑alpha dorms” where collective violence becomes both predictable and deeply ambiguous, and where post‑riot behaviors signal the emergence of a “new normal” in …

Stories From Former Foster Youth College Graduates: The Positive Impact Of K-12 Schooling And Related Educational Policy, Amanda Moon, Kristen D. Seay

Faculty and Staff Publications

Youth in foster care face disproportionate challenges when it comes to education and personal well-being. Many studies use a deficit lens when evaluating these challenges. In contrast, this study is asset-based, centering first-person accounts from five former foster youth college graduates. Using a counter-narrative framework, we conducted semi-structured interviews with participants to reveal key supports in K-12 schooling participants believed helped them achieve academic and life milestones. These were relationships with teachers and coaches, access to extracurricular activities, and literacy-rich environments. We also reviewed 11 relevant federal educational policies to identify provisions and implementation needs that aligned with participant experiences. …

Improving Lexicosemantic Impairments In Post-Stroke Aphasia Using Rtms Targeting The Right Anterior Temporal Lobe, Sophie Arheix-Parras, Sophia R. Moore, Rutvik H. Desai

Faculty Publications

Background/Objectives: Repetitive Transcranial Magnetic Stimulation (rTMS) can enhance post-stroke aphasia recovery. The right Inferior Frontal Gyrus is the most common target in rTMS studies for inhibitory stimulation. However, lexicosemantic processes involve a large brain network including the Anterior Temporal Lobe (ATL). We hypothesize that rTMS targeting the ATL will improve lexicosemantic impairments in people with post-stroke aphasia. Methods: In a Single-Case Experimental Design, three people with post-stroke aphasia with lexicosemantic impairments performed Picture and Auditory Naming and Semantic Decision tasks five times a week for one or two weeks to establish baseline scores. Then, each participant received continuous inhibitory Theta …

Meet Me At The Rock: The Behavioral Patterns Of Collective Violence In Prison, Hayden P. Smith

Faculty Publications

This study explores the behavioral dynamics of collective violence in prisons, focusing on the largest prison riot in the United States in twenty-five years at Lee Correctional Institution, using an ethnographic approach and Crime Script Analysis (CSA). The sample consisted of twenty-five incarcerated men, predominantly African American, aged 25 to 61, with extensive incarceration histories, experiences of violence, and influencer status within the prison community. It identifies key factors such as situational awareness, gang affiliations, and chronic understaffing that escalate violence. Themes include the "pressure cooker" environment, elevated tensions, and stages of confrontation, escalation, and de-escalation. A key finding is …

Police Staffing Shortages, Public Awareness, And Policy Preferences, Hunter M. Boehme, Joshua Mccrain, Scott M. Mourtgos, Brandon Tregle

Faculty Publications

Since the summer of 2020, police agencies across the United States have continued to experience police staffing shortages. The consequences of understaffed police agencies are well studied, but little is known about whether the public is aware of the staffing problem and its consequences for public safety and police behavior. This study presents a pre-registered factorial survey experiment to 3105 US adults, varying the information respondents see on the consequences of staffing shortages (e.g., public safety, police behavior) and the messenger of this information (e.g., police chief, mayor). Respondents are then asked about their levels of concern about police staffing, …

When Disasters Trigger Cyber Vulnerabilities: Mapping Physical-Digital Interdependencies In Critical Infrastructure Systems, Dikshya Panta, Sicheng Wang, Aditya Sapkota, Prakash Ranganathan

Faculty Publications

Critical infrastructure (CI) systems such as power, water, communications, and emergency services are increasingly exposed to compound risks in which natural disasters and cyber incidents interact and amplify one another. Traditional risk assessments often isolate physical and digital threats, overlooking the cascading dependencies that emerge when operational stress, emergency reconfiguration, and adversarial exploitation coincide. This study conducts a 2019–2025 scoping review and introduces a Geographic Information System (GIS) driven six-stage disaster cyber compounding framework that characterizes, maps, and operationalizes compound risk across interdependent CI sectors. The framework integrates a common operating picture, analytic situational understanding, exposure mapping, threat-fingerprint encoding, detection …

The Age And Composition Of The Voyager Seamounts: Evidence For A Long-Lived Marquesas Mantle Source, Andrea Balbas, Hannah Paradis, Kevin Konrad, Valerie A. Finlayson, Michael Bizimis, Stephanie Anderson, Christopher Kelley, Nicole A. Raineault, Beth N. Orcutt

Faculty Publications

We present new observations on the dynamics and locations of deep mantle reservoirs derived from the ages and compositions of Voyager Seamount Chain lava flows. The previously unexplored Voyager Seamount Chain trends NW–SE between the Mid-Pacific Mountains and the Northwestern Hawaiian Ridge. Volcanic samples were recovered from the chain during the Ocean Exploration Trust expedition NA134. The lava flows are alkalic to highly alkalic in composition. Ages ranged from 81 to 86 Ma (n = 8), with the oldest ages in the NW and an age-progression toward the SE. The Voyager age-progression continues southward through the Northern Line Islands …

High Frobenius Pushforwards Generate The Bounded Derived Category, Matthew R. Ballard, Srikanth B. Iyengar, Pat Lank, Alapan Mukhopadhyay, Josh Pollitz

Faculty Publications

This work concerns generators for the bounded derived category of coherent sheaves over a noetherian scheme X of prime characteristic. The main result is that when the Frobenius map on X is finite, for any compact generator G of D(X) the Frobenius pushforward F^e_*G generates the bounded derived category whenever p^e is larger than the codepth of X, an invariant that is a measure of the singularity of X. The conclusion holds for all positive integers e when X is locally complete intersection. The question of when one can take G = …

Dietary Inflammatory Index And Risk Of Colorectal Cancer In Japanese Men, Ayaka Kotemori, Kumiko Kito, Motoki Iwasaki, Taiki Yamaji, James Hébert Scd, Junko Ishihara, Manami Inoue, Shoichiro Tsugane, Norie Sawada On The Behalf Of Jphc Study Group

Faculty Publications

Background/Objectives: Unhealthy lifestyles lead to chronic low-grade inflammation, increasing the risk of colorectal cancer. Few studies in East Asia have examined the association between the dietary inflammation potential and colorectal cancer incidence. Therefore, we aimed to investigate this association further in the Japanese population. Methods: This study included 38,807 men aged 45–74 years who participated in the Japan Public Health Center-based prospective study (JPHC Study). The energy-adjusted dietary inflammatory index (E-DII) was derived from a food frequency questionnaire. Hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated using Cox proportional hazards regression models. Differences in risk due to a …

More Than A Prescription: The Need For Behavioral Theory To Guide Exercise Interventions For Osteoarthritis - A Narrative Review, Christine Pellegrini, Lisa C. Carlesso, Daniel K. White

Faculty Publications

Objective

Design

Results

Supervised Resistance Training In An Individual With Glioblastoma Undergoing Chemoradiation: A Case Report, Bruce Nakfoor, Ciaran Fairman Ph.D., Cscs, Cet

Faculty Publications

Introduction:

Exercise has demonstrated safety and efficacy in mitigating treatment-related symptoms across cancer populations; however, evidence in neuro-oncology remains limited, particularly during active chemoradiation.

Methods:

We report the case of a 63-year-old individual with newly diagnosed glioblastoma, experiencing symptoms of fatigue, balance issues, headaches, and memory loss, who initiated a 12-week supervised resistance training program during concurrent chemoradiation. Assessments at baseline and post-intervention included physical function and quality-of-life outcomes.

Results:

Adherence was 56% (20/36 sessions), and no adverse events occurred. The patient showed improvements in 6-minute walk distance and short physical performance battery score that exceeded established minimal clinically important …

Gendered Paths To Cancer Knowledge And Screening Behavior Among Black Adults: Results From A Community-Partnered Study, Carolyn M. Tucker, Guillermo M. Wippold, Kirsten G. Klein, Logan Marcum, Ji-Hyun Lee, Gonghao Liu, Staja Q. Booker, Elliott Leighton, Danielle E. Jake-Schoffman, Adrian S. Taylor, Ron Rawls, Kenyarda T. Feathers, Kevin A. Thorpe, Gwendolyn Saffo, Deborah Johnson

Faculty Publications

Background

Efforts are needed to promote cancer knowledge and the uptake of cancer screening behaviors among Black Americans. Such efforts must be customized for the unique differences in facilitators/barriers among Black men and women. The present study leverages the potential of the UF Health Cancer Institute Community-Partnered Cancer Disparities Research Collaborative (CDRC) to identify the psychosocial and structural factors associated with cancer knowledge and screening behaviors among Black American men and women.

Method

411 Black Americans participated in this cross-sectional study; 75.9% identified as cisgender women and 24.1% as cisgender men.

Results

Older age, higher education and income level were …

Strength In Numbers: Exploring The Link Between Upper Body Muscular Strength And Academic Achievement In Youth, Julian A. Reed, Marsha Dowda Drph, Russell R. Pate Ph.D.

Faculty Publications

Objectives

The purpose of this study was to examine the associations between upper body strength and endurance (UMSE) and academic achievement in youth.

Design

Cross-sectional study included 5th (N = 25,025) and 8th grade (N = 14,988) students in South Carolina.

Methods

Academic achievement was measured using a statewide assessment and classified into two categories (meets/exceeds standards or does not meet/approaches standards). Achievement in mathematics and English Language Arts was assessed. UMSE was measured as part of the South Carolina FitnessGram project and expressed as Healthy Fitness Zone (HFZ) or Needs Improvement – Health Risk. Student demographic characteristics were derived …

Crystal Structures, Optical Behavior, And Magnetic Properties In Hydrated Lanthanide Iron Sulfates, Chole Jones, Silu Huang, Tyler L. Spano, Eric A. Gabilondo, Mohammed Al-Fahdi, Kara Trim, Mary Douglas, Rongying Jin, Andrew Miskowiec, P. Shiv Halasyamani, Ming Hu, Jie Ling

Faculty Publications

Single crystals of LnFe(SO₄)₃(H₂O)₂ (Ln = La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm; compounds 1–11) and LnFe(SO₄)₃(H₂O) (Ln = Tm, Yb, Lu; compounds 12–14) were synthesized under hydrothermal conditions. Single-crystal X-ray diffraction (SCXRD) analysis revealed that the dihydrated compounds (1–11) crystallize in centrosymmetric (CS) structures, with the lanthanide ions adopting eight-coordinate geometries. In contrast, the monohydrated compounds (12–14) exhibit noncentrosymmetric (NCS) structures, where the lanthanide ions are seven-coordinated. Vibrating sample magnetometry (VSM) confirmed that compounds 2, …

Cognitive Dysfunction In Women With The Fmr1 Premutation During Midlife: The Lassi-L Reveals Curvilinear Cgg-Dependent Risk Buffered By College Education, Jessica Klusek, Jillian Gierman, Amanda Fairchild, Andreana M. Benitez, Elizabeth Berry-Kravis, Marsha R. Mailick

Faculty Publications

Background

The FMR1premutation (FXPM) is a common genetic variant (∼1:151 females) linked to increased risk for neurodegenerative disease. Midlife cognitive phenotypes remain poorly defined.

Objective

To characterize episodic memory performance in midlife FXPM women and examine potential risk moderation via genetic (i.e., FMR1CGG repeat expansion size) and environmental (i.e., college degree attainment) influences.

Methods

Eighty-eight FXPM women and 84 matched controls, aged 30–55, completed the Loewenstein-Acevedo Scales for Semantic Interference and Learning (LASSI-L), a measure of specific episodic memory processes sensitive to subtle Alzheimer's disease (AD)-related cognitive and neuropathological changes.

Results

FXPM women demonstrated deficits in proactive semantic …

Gatos – Xi. Excess Dust Heating In The Narrow-Line Regions Of Nearby Agn Revealed With Jwst/Miri, Houda Haidar, David J. Rosario, Ismael García-Bernete, Almudena Alonso-Herrero, Anelise Audibert, Steph Campbell, Chris M. Harrison, Tiago Costa, Laura Hermosa Muñoz, Françoise Combes, Dimitra Rigopoulou, Claudio Ricci, Cristina Ramos Almeida, Enrica Bellocchi, Peter Boorman, Andrew Bunker, Richard Davies, Daniel Delaney, Tanio Díaz Santos, Federico Esposito, Enrique Lopez Rodriguez, Et. Al.

Faculty Publications

We present James Webb Space Telescope/Mid-Infrared Instrument imaging of eight nearby active galactic nuclei (AGN) from the GATOS (Galactic Activity, Torus, and Outflow Survey) survey to investigate the physical conditions of extended dust in their narrow-line regions (NLRs). In four galaxies (ESO 428–G14, NGC 4388, NGC 3081, and NGC 5728), we detect spatially resolved dust structures extending ∼100–200 pc along the NLR. In these systems, we find a strong link between the morphology of the dust, the radio ejecta, and the coronal [Si VI] emission, implying that dust carries imprints of the processes shaping the NLR. Using spatially resolved …

Statewide Sanctuary Policies And Female Homicide Rates, 2016–2021, Kaitlin M. Boyle, Sophia Shaiman, Veronica Valencia Gonzalez, Wendy Regoeczi

Faculty Publications

The current study examines whether state immigration enforcement policies, such as sanctuary policies that limit local police cooperation with federal immigration enforcement, are associated with female homicide rates in the United States (2016–2021). We examine crude female homicide rates from the National Violent Death Reporting System (N = 244 state-years), which we stratify by race and ethnicity (Hispanic of all races, non-Hispanic Black, and non-Hispanic White) and victim–suspect relationship (domestic vs. stranger homicide). State-years with policies that are more protective of immigrants were not associated with higher homicide rates for any group. Instead, state-years with these policies reported significantly lower …

Pdr-Stgcn: An Enhanced Stgcn With Multi-Scale Periodic Fusion And A Dynamic Relational Graph For Traffic Forecasting, Jie Hu, Bingbing Tang, Langsha Zhu, Yiting Li, Jianjun Hu, Guanci Yang

Faculty Publications

Accurate traffic flow prediction is a core component of intelligent transportation systems, supporting proactive traffic management, resource optimization, and sustainable urban mobility. However, urban traffic networks exhibit heterogeneous multi-scale periodic patterns and time-varying spatial interactions among road segments, which are not sufficiently captured by many existing spatio-temporal forecasting models. To address this limitation, this paper proposes PDR-STGCN (Periodicity-Aware Dynamic Relational Spatio-Temporal Graph Convolutional Network), an enhanced STGCN framework that jointly models multi-scale periodicity and dynamically evolving spatial dependencies for traffic flow prediction. Specifically, a periodicity-aware embedding module is designed to capture heterogeneous temporal cycles (e.g., daily and weekly patterns) and …

Fatigue Crack Length Estimation Using Acoustic Emissions Technique-Based Convolutional Neural Networks, Asaad Migot, Ahmed Saaudi, Roshan Joseph, Victor Giurgiutiu

Faculty Publications

Fatigue crack propagation is a critical failure mechanism in engineering structures, requiring meticulous monitoring for timely maintenance. This research introduces a deep learning framework for estimating fatigue fracture length in metallic plates through acoustic emission (AE) signals. AE waveforms recorded during crack growth are transformed into time-frequency images using the Choi–Williams distribution. First, a clustering system is developed to analyze the distribution of the AE image-based dataset. This system employs a CNN-based model to extract features from the input images. The AE dataset is then divided into three categories according to fatigue lengths using the K-means algorithm. Principal Component Analysis …

Host-Derived Interleukin-1Α Drives Tumor Immunosuppression By Reprogramming Tumor-Associated Myeloid Cells, Manikanda Raja Keerthi Raja, Gourab Gupta, Grace Atkinson, Katie Kathrein, Alissa R. Armstrong, R. Michael Gower, Igor Roninson Ph.D., Eugenia Broude, Mengqian Chen Ph.D., Hao Ji, Chang-Uk Lim, Hongjun Wang, Daping Fan, Peisheng Xu Ph.D., Hexin Chen

Faculty Publications

Myeloid cells are highly heterogeneous and play key roles in establishing an immunosuppressive tumor microenvironment (TME), but the mechanisms controlling their differentiation remain unclear. Using orthotopic murine breast cancer models, we investigated how interleukin-1α (IL-1α) influences this process. Deletion of host Il1α resulted in rejection of transplanted tumors. Single-cell RNA sequencing identified CX3CR1⁺ macrophages as the main source of IL-1α in the TME. Il1α deficiency disrupted monocyte–macrophage differentiation, reducing CX3CR1⁺ macrophages and increasing iNOS⁺ macrophages. Myeloid cells from Il1α⁻/⁻ hosts induced lower PD-1 and CTLA-4 expression in co-cultured CD8⁺ T cells than those from wild-type hosts. CellChat analysis showed …