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We Need Proper Case Reports And Data To Interpret Reports Of Peri-Operative Deaths In Venezuelan Children, J Robert Sneyd

More Faculty of Health Research

A report describing several perioperative deaths amongst Venezuelan childrenraises substantial clinical concern and demands scientific scrutiny. Rozo L, FrancoOH, Bonilla AJ. Mitochondrial genetic susceptibility to anesthetic neurotoxicityin Venezuelan pediatric patients: A Call for vigilance and further research.https://doi.org/10.29328/journal.ascr.1001091. Archives of Surgery and Clinical Research.2025;9(2):042-4. According to the authors, the affected children experienced severe brain injuries or death following routine surgeries under general anaesthesia with sevoflurane, leading to the hypothesis that an inherited mitochondrial DNA variant may underlie a lethal drug–genome interaction. Whilst any paediatric peri-operative death warrants urgentinvestigation, the extraordinary nature of this claim requires equally compelling evidence. At present, the …

Exploring The Clinical Utility Of The Adult Social Care Outcomes Toolkit For Use In Occupational Therapy Practice In Adult Social Care: A Qualitative Study, Rachel Cox, Sarah Reynolds

School of Health Professions

AbstractBackground/Aims Promoting the wellbeing of service users is a core focus in occupational therapy and adult social care. Despite a professional, policy and legal mandate to provide evidence of the effectiveness of occupational therapy interventions in adult social care, few local authority teams in England use standardised outcome measures to assess the impact of interventions on wellbeing. One local authority’s occupational therapy service in England sought to address this by introducing the Adult Social Care Outcomes Toolkit. This study explored the Adult Social Care Outcomes Toolkit’s clinical utility (usefulness) in this setting. As the Adult Social Care Outcomes Toolkit has …

Headwater Drainage Evolution In A Foreland Basin System Usingsurfaces And Topographic Metrics (Zadorra River, Upper Ebro,Spain), Ivan Martin-Martin, Pablo G. Silva, Martin Stokes, Antonio Tarriño, Benito Abalos, Fernando Sarrionandia

School of Geography, Earth and Environmental Sciences

Foreland basins are elongate depressions that develop around the flanks of collisional mountain ranges. Rivers are key agents that shape their landscape geomorphology and evolution. Their upstream headwater areas are geomorphologically dynamic locations prone to drainage network reorganisations via headward erosion by the foreland axial river. They are complex areas to investigate due to geological complexity, the absence of fluvial deposits and long timescales over which drainage evolution occurs. In this study, we examined the Zadorra River, a headwater tributary to the Ebro River that preserves a diverse number of different geomorphological features that record long-term drainage evolution. Four key …

Unreliable Landmarks Disrupt Egocentric Navigation In Desktop Virtual Environments, Rory Baxter, Alastair Smith

School of Psychology

Navigation relies on the integration of both allocentric (world-centred) and egocentric (navigator-centred) spatial cues. An influential real-world navigational task dissociated allocentric place learning from egocentric path integration, highlighting the decline in allocentric cue use associated with cognitive ageing. This report describes a novel computer-based replication of this task, designed as a remote test of navigational ability, that also addresses potential confounds in the original task, including tighter control of spatial cue competition. In Experiment 1a, participants learned a location signalled either by landmark cues in the absence of a stable starting position (Allocentric), distance and direction from the starting position …

Toward A Roadmap For Addressing Today's Health Dilemma-The 101-Statement Consensus Report, Katharina C. Wirnitzer, Mohamad Motevalli, Derrick R. Tanous, Clemens Drenowatz, Maximilian Moser, Holger Cramer, Thomas Rosemann, Karl-Heinz Wagner, Andreas Michalsen, Beat Knechtle, Zlatko Fras, Merel Ritskes-Hoitinga, Adilson Marques, Natasa Fidler Mis, Fatima C. Stanford, Christian Schubert, Nandu Goswami, Claus Leitzmann, Per Morten Fredriksen, Gerhard Ruedl, Doris Wilflingseder, Rodrigo A. Lima, Christian Kessler, Michael Jeitler, Naim A. Khan, Hassan Joulaei, Maryam Fatemi, Andrew Knight, Karl W. Kratky, Kara K. Palmer

School of Health Professions

Importance: In recent decades there has been an expansion in the quantity and quality of scientific findings and guidelines on different health topics to promote individual and public health status. Reports also indicate that there has been a simultaneous increase in the financial burden of disease, including trillions spent on healthcare resources by governments worldwide (predominantly in developing countries) to address health concerns. At the same time, personal health behavior is well-known as efficient and cost-free, holding four times the potential to prevent early death compared to health care. Despite this knowledge, data show that the increasing prevalence of unhealthy …

Teaching Dental Public Health Using A Socially Accountable Approach, Anastasios Plessas, Martha Paisi, Nilufar Ahmed, Zoe Brookes, Robert Witton

Peninsula Dental School

Traditional approaches to dental public health education across Europe remain dominated by lecture-based learning which often fail to cultivate critical reflection on inequities, privilege, and the broader social determinants of oral health. Social accountability has been recognized as core competency for dental graduates, requiring dental professionals to align professional practice with the health needs of communities. Community-engaged education is a promising pedagogical model that situates learning within real-world contexts, fosters empathy, cultural competence, and advocacy skills, and strengthens professional identity formation. This paper discusses the Peninsula Dental School’s innovative model, which integrates community engagement, reflective practice, and interprofessional education within …

Patient-Reported Experience Measures For Person-Centred Care: A Survey For The Development And Psychometric Testing Of A Targeted Measure, The Epcc-14, Ryan Statton, Lena Rosenlund, James Close, Anna Dencker, Helen Lloyd

School of Psychology

Objectives This study aims to develop a robust, targeted measure of patient experiences of person-centred care (PCC), informed by the lived experiences of patients with chronic illness using the psychometric theory of Rasch measurement. Design The Rasch measurement model was used to analyse the psychometric functioning of 57 candidate items and select appropriate items for a targeted measure. Setting Participants were recruited from Prolific.com, having experience of both chronic or long-term illness and first-hand experience of primary or secondary care in the UK healthcare setting and completed a survey containing PCC items and descriptors of healthcare experience. Participants Data from …

Talking Sudep: Gaps, Confidence And Training Needs Among Spanish Epilepsy Professionals, Rohit Shankar

Peninsula Medical School

Objective: Sudden Unexpected Death in Epilepsy (SUDEP) is a major cause of epilepsy-related mortality, yetdiscussions about SUDEP in clinical settings remain inconsistent. This study aimed to assess the perspectives,practices, and barriers related to SUDEP counselling for epilepsy professionals in Spain.Methods: A cross-sectional online survey of 17 Likert style questions was disseminated via the Spanish EpilepsySociety between September 2023 and February 2024 to epilepsy professionals in Spain using a nondiscriminatory exponential snowballing technique leading to non-probability sampling. The survey was a validated instrument previously employed in similar international studies. Questions revolved around SUDEPcommunication and counselling. Descriptive and comparative analyses were conducted.Results: …

Global, Regional, And National Burden Of Cardiovascular Diseases And Risk Factors In 204 Countries And Territories, 1990-2023, Global Burden Of Cardiovascular Diseases And Risks 2023 Collaborators, Benjamin A. Stark, Nicole K. Decleene, Emily C. Desai, Johnathan M. Hsu, Catherine O. Johnson, Laura Lara-Castor, Kate E. Legrand, Prof Bhoomadevi A, Mohammad Amin Aalipour, Hasan Aalruz, Bedru J. Abafita, Ukachukwu O. Abaraogu, Mohammad Abavisani, Nasir Abbas, Madineh Abbasi, Mohammadreza Abbasian, Hedayat Abbastabar, Abdallah H.A. Abd Al Magied, Samar Abd Elhafeez, Prof Ahmed Abdelalim, Omar M. Abdelfattah, Prof Reda Abdel-Hameed, Mahmoud Abdelnabi, Prof Wael M Abdel-Rahman, Parsa Abdi, Wakgari Mosisa Abdisa, Daba Abdissa, Arman Abdous, Mujahid Abdullah

Peninsula Medical School

Background: Cardiovascular diseases (CVDs) are the leading cause of mortality and are among the foremost causes of disability globally. CVD burden has continued to increase in most countries since 1990, with trends driven by changing exposures to harmful risk factors, population growth, and population aging. Objectives: We report estimates of global, national, and subnational CVD burden, including 18 subdiseases and 12 associated modifiable risk factors. We analyzed change in CVD burden from 1990 to 2023 and identified drivers of change including population growth, population aging, and risk factor exposure. Methods: The Global Burden of Disease (GBD) 2023 study, a multinational …

Public Priorities For The Post-War Reconstruction Of Ukraine’S Energy Sector., Olena Savchenko, Vasyl Zhelykh, Khrystyna Kozak, Omar Al-Hafith, Joao Alencastro, Olugbenga Oladinrin, Ademola Adebayo Akinbami

School of Art, Design and Architecture

The Russian invasion of Ukraine has resulted in the large-scale destruction of many cities across the country. Although the war has not yet ended, there will come a time when the conflict concludes, and reconstruction efforts will begin. Global experiences show that the first step in preparing for post-war reconstruction is to assess the level of destruction. Additionally, it is essential to understand people’s needs and priorities. Otherwise, reconstruction activities may fail to address the existing challenges and actual needs of local communities. This study aims to explore post-war reconstruction priorities of Ukrainians, with special focus on the energy sector …

Investigating The Performance Of Fibre Reinforced Plastic Repairs To Timber Marine Civil Structures At His Majesty’S Naval Base Devonport, Anna G. Walther

The Plymouth Student Scientist

This paper explores Fibre Reinforced Plastics (FRPs) as a composite repair method to restore and enhance mechanical properties of timber marine civil structures within the context of His Majesty’s Naval Base (HMNB) Devonport. To support this, timber composite specimens were manufactured and subjected to flexural and compressive strength tests. Comparisons are made to literature sources for initial predictions, in addition to hand calculations and Finite Element Analysis (FEA) models on SolidWorks. Data demonstrates that FRPs can be used as a cost-effective and beneficial method of repairing timber marine structures. Experimental data demonstrates an ability to restore compressive strength parallel to …

The Effect Of Exercise On Dynamic Balance And Walking Endurance In Patients With Relapsing Remitting Multiple Sclerosis: A Systematic Review And Meta-Analysis, Connor Gibson

The Plymouth Student Scientist

Background: Multiple Sclerosis (MS) affects 2-3 million people worldwide, with relapsing remitting MS (RRMS) accounting for 75% of new cases. Exercise has been proposed to alleviate symptoms and enhance quality of life. This meta-analysis aims to evaluate the effect of exercise on dynamic balance and walking endurance in RRMS patients.

Methodology: A comprehensive PubMed search identified exercise interventions that specifically measured walking endurance via the 6-minute walk test (6MWT) and dynamic balance through the timed up-and-go (TUG) test. Mean and standard deviation values at baseline and post-intervention were extracted for analysis. For subgroup comparisons, interventions were classified as either lower …

Mechanical Properties Of Sintered Lunar Regolith Simulant Lhs-1 When Moulded Under Different Temperatures And Time At Constant Pressure, Jasper Stevens

The Plymouth Student Scientist

Humans are returning to the Moon for the first time since 1972. And when they arrive, In-Situ resource Utilisation (ISRU), the philosophy of using local materials and resources will be required to help maintain a more permanent presence on the Moon. Lunar regolith is the homogenous layer of inorganic ‘soil’ that covers nearly the entire Lunar surface, making it the most readily available material for ISRU. This report investigates the effect of changing time and temperature applied to samples of lunar regolith simulant LHS-1 formed as freestanding samples under constant pressure, before sintering into a consolidated sample. Compressive testing on …

Application And Suitability Of Using Paraffin Wax In A Thermal Store For Residential Buildings In The Uk, Cameron Edwards

The Plymouth Student Scientist

Heating costs and pressure to reduce carbon emissions are rapidly increasing. A possible solution is the implementation of efficient energy storage systems for UK residential buildings. Paraffin wax-based thermal energy storage offers a promising solution due to its low-cost, high-energy density, and long-term stability. However, its low thermal conductivity significantly reduces its practicality. Multiple upgrades, such as implementing highly conductive particles and nanotubes, have been previously studied but lack the inclusion of cost-effectiveness and real-world feasibility. This paper reviews current research and develops a numerical model using the Enthalpy method and a 2D finite difference approach, utilised in Python. It …

Assessing Cognitive Performance And Comfort Under Simulated Mri Noise And Its Association With Autistic Like Traits, Jasmin Woodhouse

The Plymouth Student Scientist

Magnetic Resonance Imaging (MRI) scanners produce noise levels between 65 and 130 decibels. This noise, alongside the broader sensory environment of the MRI, poses challenges for individuals with autism spectrum disorder (ASD). Noise exposure has been shown to impair task performance, increase errors, and affect brain activity. ASD traits are associated with decreased sound tolerance, including hyperacusis, misophonia, and phonophobia. In ASD, noisy environments disrupt inferior frontal gyrus function, impairing task response and brain connectivity. Given this, it’s crucial to understand how these sensory challenges specifically affect ASD experience and performance during MRI procedures. This study assesses cognitive performance and …

Microplastic Accumulation In A Mixed Species Seagrass Bed In The Tamar Estuary, Torpoint, Cornwall, Uk, Hannah G. Phillips

The Plymouth Student Scientist

Microplastic pollution is a major threat to UK seagrass habitats, which provide essential ecosystem services by supporting biodiversity and economically valuable fish species. Seagrass beds, acting as long-term ‘sinks’ for microplastics due to their dense canopies face a high risk of degradation. This study investigated microplastic accumulation in the sediment of a mixed species (Zostera marina and Nanozostera noltei) seagrass bed in the Tamar Estuary, Southwest England, considering the effect of sediment particle size. Microplastics were extracted by density separation using Sodium Bromide (NaBr) and sediment particle size analysis was used to investigate the seagrass bed sediment. FT-IR …

Detecting Concealed Autobiographical Information Using Feedback And Recognition P300 Erps. Exploring The Impacts Of Changed Feedback Proportions And Social Pressure In The Fcit, Cerys Parkinson-Johns, Grace Shillito, Callum Starr, Rosemary Wilmshurst

The Plymouth Student Scientist

The feedback concealed information test (fCIT) incorporates feedback on memory concealment performance within the classical CIT. Providing feedback facilitates the analysis of feedback related ERPs in addition to initial recognition related ERPs within each trial. Combining multiple ERPs aims to improve the reliability and efficiency of the test. The present study investigated the efficiency of combining recognition and feedback related P300 ERPs in detecting concealed birthdates in a personal item fCIT. Feedback proportions were altered to improve believability in feedback. Detection efficiencies using combined P300 amplitudes within this altered paradigm and the impacts of social pressures on feedback P300s were …

A Generational Analysis Of Psychological Motivators Influencing Seafarer Retention, Perisah Tahanci

The Plymouth Student Scientist

Seafarer retention is a growing concern amid increasing global demand for skilled maritime officers. This study investigates the generational differences in psychological motivators influencing seafarer retention, where ‘psychological motivators’ encompass the cognitive factors, including mental and physical wellbeing, work-life balance, compensation, working conditions, and external factors, that shape retention intentions. A cross-sectional survey (n=214) combined quantitative and qualitative analyses to systematically assess how the perceived importance and experience of these factors vary across Generation Z, Millennials, Generation X, and Baby Boomers.

The findings reveal that significant generational differences exist across most factors, particularly in work-life balance and mental wellbeing, in …

Effects Of Maternal Hypoxia On Developmental Plasticity, Matilda Powell

The Plymouth Student Scientist

Developmental plasticity enables organisms to adjust phenotypes to environmental cues, yet the transgenerational consequences of maternal effects remain unclear. Hypoxia, intensified by climate change and eutrophication, triggers taxon-specific plastic responses. Daphnia magna exhibits plasticity dependence, making it a critical model species for studying environmental responses. While maternal hypoxia (MH) influences generational plasticity, its long-term impacts on reproductive and physiological traits across generations remain insufficiently explored, limiting predictions of species resilience to environmental change. This study employed a controlled two-generation design: gravid F0 adults were exposed to moderate hypoxia (40% air saturation) or normoxia (100% AS) for 8 days, with reproductive …

Examining The Correlation Between The Competing Values Framework And Other Scales Of Measure, Jesina Mistry

The Plymouth Student Scientist

Organisational culture is widely acknowledged as a critical determinant of performance and employee engagement, yet debates persist regarding how best to conceptualise and measure it. This study examines the construct validity of two widely used diagnostic tools: the Competing Values Framework (CVF) and the Organisational Culture Inventory (OCI). While the CVF has achieved broad application across sectors, questions remain regarding its empirical grounding and cross-cultural relevance. Using a quantitative, cross-sectional design, this research investigates the degree of convergence between CVF and OCI dimensions within a diverse UK-based sample spanning multiple industries and job levels. In addition, it incorporates three underexplored …

Assessing Overtopping Hazard To The Dawlish Railway Line: Integrating Wave Propagation Models For Coastal Metocean Condition Analysis, Matthew R. Owen

The Plymouth Student Scientist

With rising sea levels and increasingly frequent and intense storms due to climate change, coastal communities and structures are at an increasing risk of overtopping occurring. This study investigates the coastal metocean conditions responsible for overtopping at coastal defences, using Delft3D to model these processes with Dawlish as the case study site. Wave propagation models were employed to simulate metocean conditions from December 2021 to March 2022 to compare inshore wave conditions, alongside water level and wind parameters, with observed overtopping data from the WireWall system installed on top of the seawall, next to the railway line. The results indicate …

From Sea To Shore: Wind-Driven Aerosolization Of Harmful Algal Bloom Toxins In Qingdao, China, During The Winter Of 2019, Madeleine K. Martin

The Plymouth Student Scientist

In Qingdao, China, harmful algal blooms (HABs) are a known hazard to coastal communities. Aerosolization is a mechanism by which HAB toxins can be transported from the sea surface into the air when wind speeds exceed 5 m/s. Previous studies have analysed the composition of aerosolized marine biotoxins (AMBs) in the breathable air surrounding Qingdao, but the role of the onshore winds in transporting them there would benefit from further research. Ciguatoxin is one such AMB, and this study analyses the role of onshore winds in aerosolizing and transporting toxins inland from a ciguatoxin bloom in Aoshan Bay, Qingdao during …

Comparing The Effect Of Marine Aquarium Salts And Sodium Chloride On Vicia Faba Stress Response, Bethany Smerdon

The Plymouth Student Scientist

Soil salinity is a major abiotic stressor limiting agricultural productivity. Many salt stress studies rely solely on sodium chloride (NaCl) solutions, despite not accurately representing natural saline conditions. This study investigated the stress response of Vicia faba to NaCl and marine aquarium salt (MAS) solutions to assess the impact of a more complex ionic composition on plant physiology. Plants were cultivated in a controlled greenhouse and treated with either NaCl, MAS, or rainwater (control) for up to 55 days. Growth parameters, water status, proline accumulation, chlorophyll content, and root nodule weight were measured to evaluate stress responses. The results indicate …

Evaluating The Impacts Of The Invasive Macrophyte, Crassula Helmsii, On Macroinvertebrate Assemblages At Small Spatial Scales In Lentic Ecosystems, Keivan Rakhshani

The Plymouth Student Scientist

Freshwater ecosystems cover only three percent of the Earth’s surface yet support approximately ten percent of global biodiversity. These systems play a crucial role in maintaining native biodiversity across spatial and temporal scales but are increasingly threatened by anthropogenic stressors, including habitat modification, pollution, and biological invasions. The introduction of non-native macrophytes can fundamentally alter community structure by modifying habitat complexity, resource availability, and competitive interactions. This study assesses the impact of the invasive macrophyte Crassula helmsii on macroinvertebrate assemblages at small spatial scales within partially invaded ponds in Dartmoor National Park, southwest England. Three ponds with varying degrees of …

Assessing Jasmonic Acid Seed Priming In Brassica Napus: Impacts On Aphid Herbivory Resistance And Seedling Fitness Trade-Offs, Daisy Stradling

The Plymouth Student Scientist

Jasmonic acid (JA) is a key phytohormone involved in plant defence, offering a potential sustainable alternative to conventional chemical pesticides. While JA seed priming has been explored as a strategy to enhance herbivore resistance in certain plant families, its potential fitness trade-offs remain poorly understood. This study investigates the effects of JA seed priming on Brassica napus (oilseed rape), a key agricultural crop, focusing on seedling fitness and resistance to Brevicoryne brassicae (mealy cabbage aphid), a major agricultural pest. To assess potential fitness trade-offs, seeds were primed with 75 µM JA, and multiple seedling fitness parameters were measured. Germination monitoring …

The Lived Experience Of Uk Students Transitioning To University With Type 1 Diabetes Mellitus, Emelia C. Steel

The Plymouth Student Scientist

Transitioning from secondary school to university is a high-risk period for managing type 1 diabetes among UK students. Canadian research identified limited systemic support during this period. However, there is a lack of qualitative insight into how students navigate this transition in the UK, where healthcare and education systems differ. This study addressed that gap through semi-structured interviews to understand what makes this transition particularly challenging and what support students would find most helpful. A purposive sample comprised seven participants aged 19–29 from four UK universities. Thematic analysis, guided by Braun and Clarke’s (2022) six-phase framework, identified three superordinate themes: …

Investigating Deception-Related Moral Conflict Using Electroencephalography (Eeg) Hyperscanning, Madie Ades

The Plymouth Student Scientist

Amplitudes of the early Bereitschaftspotential (BP) and deceptive behaviour have been theorised to be modulated by moral conflict experienced when making deceptive decisions, as the decision to lie can depend upon our moral perceptions of what is right and wrong (Panasiti et al., 2014). However, most Electroencephalography (EEG) deception paradigms lack ecological validity by utilising instructed lying and failing to incorporate genuine social interaction (Ganis & Keenan, 2009) and therefore may fail to elicit genuine moral conflict. The present study combined hyperscanning EEG with a competitive two-player social game, where participants freely chose to respond accurately or deceptively for …

Reading Ability Underlies The Composite Effect For Arabic Words, Rayan Kouzy, Zahra Hussain

School of Psychology

The composite effect, originally demonstrated for faces, has recently been shown to suggest holistic processing of words. The effect is associated with reading fluency in Latin script, but not in nonalphabetic Chinese script, suggesting that script properties influence its relationship with reading expertise. We measured the composite effect for Arabic, a visually complex alphabetic script that offers a useful contrast against Latin and Chinese. Arabic-English bilinguals (N = 24), and English-only readers (N = 22) completed a composite effect task, in which they judged whether the left or right halves of word pairs were the same or different. The unattended …

The Use Of Alcohol-Free And Low-Alcohol Drinks In Pregnancy In The Uk, Kate Maslin, Heather Hopper, Jill Shawe

School of Nursing and Midwifery

Alcohol-free drinks [beers, ciders, wines, and spirits containing <0.05% alcohol by volume (ABV)], and lowalcohol drinks (between 0.05% and 1.2% ABV) are increasingly available and may be used as a harm reduction measure. However, it is not known what pregnant women think and feel about these drinks and how regularly they are consumed before and during pregnancy. A cross-sectional online survey was developed and piloted. Women ≥18 years in the UK who were pregnant, or recently pregnant, were recruited via targeted social media advertising. Of the 2092 respondents, 47.8% (n ¼ 1001) were currently pregnant; 55.7% (n ¼ 1167) were between 25 and 34 years, 90.0% were White (n ¼ 1881); 6.1% (n ¼ 128) were drinking alcohol at “increasing risk” levels (>14 units/week) before pregnancy. During pregnancy, 13.5% (n ¼ 282) consumed alcohol, which was more common in the increasing risk category (P < .01). Alcohol-free or low-alcohol drinks were consumed by 71.3% (n ¼ 1491) of respondents during pregnancy; 91.4% of the increasing risk category versus 69.9% of the lower risk category (P < .01). The most common reasons for consuming alcohol-free or low-alcohol drinks were “to choose a safer alternative” (71.9%, n ¼ 1073) and “to feel included in social events involving alcohol” (68.8%, n ¼ 1026). More than half of respondents (56.7%) thought there was insufficient information available about consuming alcohol-free and low-alcohol drinks during pregnancy, with internet searching the primary source of information. Although alcohol-free and low-alcohol drinks are commonly consumed during pregnancy, there are some safety concerns. Their role as a harm reduction measure in those who are drinking alcohol at increasing risk levels prepregnancy needs further investigation.

A Pilot Study Investigating The Impact Of Cigarette Smoking On Oral Nitrate-Reducing Bacteria Within The Oral Microbiome, Tomas L Nicholas, Raul Bescos, Amazon Doble, Katie Muddiman, Paul Kellett, Abish S Stephen, Lisa Du Toit, Robert Witton, Zoë Ls Brookes

Peninsula Dental School

ObjectiveTo evaluate the impact of cigarette smoke on the activity and abundance of oral nitrate reducing bacteria (ONRB) in vivo and in vitro.Materials and MethodsAn observational study comprising of eleven cigarette smokers and thirty-one non-smokers. A saliva and nitrate-rinse sample were collected from each participant to analyse salivary biomarkers and the activity and abundance of ONRB in vivo. Additionally, the effect of cigarette smoke on the nitrate-reducing activity of four Rothia species was investigated in vitro.ResultsThe beta diversity of the oral microbiome was significantly different between smokers and non-smokers (P = 0.0063). Neisseria flavescens and Actinomyces oris were significantly higher …