Digital Commons Network^™

Scottish Marine Renewable Energy: U.S. Approaches For Development Of Wave And Tidal Technologies, Stella Kathryn Fairall

Honors Theses and Capstones

Marine renewable energy, energy harnessed from various ocean resources such as waves, tides, currents, thermal gradients, and marine streams, has incredible potential in the United States. Several European countries have realized the potential of the resource in the North Sea and Atlantic and have created incredibly successful industries already. The United Kingdom, particularly Scotland, is the world leader because of the comprehensive policy, labor force, and markets it has established in order to make MRE a competitive energy source. In the United States, the industry is immature, underfunded, and lacks policy support. In order to establish itself as a world …

Does The Emigration Of Skilled Labor From Hungary Merit Being Called A Brain Drain?, Alexa Marie Mayo

Honors Theses and Capstones

No abstract provided.

Engineering Thermostable Regulators For Inducible Gene Expression In Thermophiles, Connor M. Joyce

Honors Theses and Capstones

Thermophilic bacteria have attracted research interest due to their ability to grow at high temperatures ranging from 45 °C to 75 °C with some extreme thermophiles able to survive nearly boiling temperatures. They are valuable sources of thermostable biocatalysts and many have great potential as industrial hosts for biofuel production because fermentation at high temperatures has advantages such as reduced processing costs and lower risk of contamination compared with mesophilic bacteria. For example, some Geobacillus species has been shown to have high solvent tolerance, making them good candidates as host for alcohol production. To develop them as industrial hosts, large …

Injectable Gelatin-Silk Fibroin Composite Hydrogels For In Situ Cell Encapsulation, Ryann D. Boudreau

Honors Theses and Capstones

Hydrogels are widely used tools for tissue engineering and regenerative medicine. Characterized as biofunctional, water-based polymer matrices with tunable mechanical properties, hydrogels have promising but limited applications in biomedical engineering, due to poor and static matrix strength. Here we plan to rectify this issue by introducing a new hydrogel made from a composite of gelatin and silk fibroin crosslinked by microbial transglutaminase (mTG) instantly and beta sheet formation gradually, respectively. This interpenetrating network (IPN) shows enhanced mechanical stiffness and strength compared to gelatin hydrogels, and is capable of encapsulating human cells with high viability demonstrated by the encapsulation of human …

Cross-Model Parameter Estimation In Epidemiology, Julia R. Fitzgibbons

Honors Theses and Capstones

No abstract provided.

Mimicking The Effect Of Prolactin On Stat3/Stat5 Activity In Breast Cancer, Jennifer N. Barbuto, Sarah R. Walker

Honors Theses and Capstones

Signal transducers and activators of transcription (STAT) 3 and 5 are commonly constitutively activated in breast cancer. STAT5 can outcompete STAT3 and reduce cell proliferation and metastasis. STAT5 activation is stimulated by prolactin, a natural hormone that can be harmful at high levels. The aim of this study is to identify some possible previously developed drugs that mimic the effect of prolactin and STAT5 without the added risk in MDA-MB231 breast cancer cells. Using the CLUE database query app and STAT5 up- and downregulation signatures, three drugs (X, K, and M) were chosen based on their similarity in signatures to …

Occurrence Of Pulmonary Arteritis With Lungworm Infections In Gray Seals From New England, 2013-2020, Jillian R. Broadhurst, Inga Sidor Dvm, Ms, Dacvp

Honors Theses and Capstones

Gray seals (Halichoerus grypus) are a common pinniped species in New England coastal waters. Parasitic infection is an important cause of mortality for this species, and lungworm species are frequently implicated in cases of respiratory infection, especially in pups and weanling seals. The most common of these is Otostrongylus circumlitus, a large nematode found in the bronchi and bronchioles of affected seals. The full life cycle is unknown but its presence in lungs and sometimes pulmonary arteries suggests that migration via arterial circulation occurs. Signs and microscopic findings are widely variable; our cases included dyspnea, bloody mucoid …

Foodborne Pathogens And Water Quality In Commercial-Scale Aquaponic Systems With Rapid Micro Screen Solids Removal, Sean Zachary Fogarty

Master's Theses and Capstones

To meet global nutritional needs, humans need to produce an ever-increasing amount of food with dwindling natural resources. Demand for nutritious foods, particularly lean meat, fruits, and vegetables, is rapidly accelerating. To increase the intensity of production and utilize non-arable land, many growers are turning to controlled environment agriculture systems like hydroponic crop production and aquaculture (fish farming), but these are inherently inefficient in terms of water and nutrient utilization. Aquaponics is a relatively novel agricultural method that combines hydroponic and aquacultural production models to mitigate many of the costs—both realized and implicit, environmental and economic—associated with either production model …

Genomic And Physiological Bioremediation Characteristics Of The Actinobacteria Frankia, Erik Swanson

Doctoral Dissertations

Introduction: Frankia is a genus of symbiotic, nitrogen fixing actinobacteria found in the soil and root tissue of actinorhizal plants throughout the world. Both Frankia and actinorhizal plants are resistant to a wide range of environmental and chemical stresses including heavy metals, high salinity, and drought. This makes Frankia and the actinorhizal symbiosis an interesting candidate for bioremediation applications. However, the microbial community found in the actinorhizal rhizosphere and surrounding soil has been implicated in some of the symbiotic and resistance traits attributed to Frankia. This necessitates thorough investigation of the genomic and genetic components of any bioremediation traits possessed …

From An Insect To The Classroom: Evolutionary Insights Into The Ecological And Behavioral Impacts Of Experience, Brooke Kathleen Woelber-Kastner

Doctoral Dissertations

Organisms exhibit suites of life history traits which enable them to respond to environmental stimuli and increase fitness. However, the relationship between organism traits and the environment is not unidirectional, rather they are in a state of constant interaction. To further our understanding of the interactions between the environment and organism phenotypes, this dissertation focuses on a group of insect scavengers, the burying beetles (Coleoptera: Silphidae: Nicrophorus) which utilize carrion to initiate reproduction and feed developing young. Here I apply ecological and molecular methodologies to demonstrate that behaviors provide an important ecological service by sequestering carrion in forest ecosystems. I …

Becoming Scientists: Undergraduate Students' Literacy Activities In Laboratory Education, Allison Giannotti

Doctoral Dissertations

In laboratory courses across universities, undergraduate students are learning to act, think, and write like professional scientists. Yet, despite the centrality of language and literacy in laboratory experiments, the process of becoming scientifically literate remains ambiguous. Thus, my dissertation examines how university laboratory courses foster undergraduate students’ emergence as literate professionals and competent communicators in science, technology, engineering, and math (STEM) fields. In particular, it examines undergraduate students’ literate practices, how they change over time, and how they are affected by material and experimental processes in three sequenced, chemistry laboratory courses. Through laboratory observations and interviews with undergraduate students, faculty, …

Carbon Emissions From Streams And Rivers: Integrating Methane Emission Pathways And Storm Carbon Dioxide Emissions Into Stream And River Carbon Balances, Andrew Robison

Doctoral Dissertations

River networks play an important role in elemental cycling at watershed, regional, and global scales. They not only serve as pipes that transport elements from land to sea, but also as complex processers that can significantly modify the timing, form, and magnitude of these elemental fluxes. For example, recent studies have shown that river networks contribute significantly to the global carbon cycle by emitting greenhouse gases like carbon dioxide (CO¬2) and methane (CH4) to the atmosphere. However, we currently do not fully understand the controls on GHG emissions from river networks, particularly what drives the variability of emissions across space …

Utilization Of Exotic Germplasm In A Cucurbita Breeding Program, Andrew Burkeland Ogden

Doctoral Dissertations

Crops in the Cucurbitaceae plant family are an important part of agricultural systems worldwide. This family includes economically important crops such as watermelon, cucumber, pumpkins, melons, and squash. These crops are plagued by a wide variety of both insect and plant pathogen pests. Breeding for genetic resistance to these pests represents a key component of many cucurbit breeding programs in both public and private sectors. Sources for disease and pest resistance often include wild species and landraces found across the world. Often, such species show poor adaptability to temperate climates. This limits plant breeders’ abilities to utilize exotic germplasm and …

Synthesis Of Thioacids And Dicarboxylic Acids Derived From Open Β-Lactams For Inhibition Of Metallo-Β-Lactamases, Junfu Chen

Doctoral Dissertations

Antibiotics are important substances that have been used in clinical treatment for over 70 years. Because of overuse and misuse of antibiotics, bacteria developed resistance. However, bacterial resistance towards antibiotics, especially the β-lactam antibiotics, is becoming increasingly threat. Currently, the β-lactam antibiotics are seriously threatened by β-lactamases. Metallo-β-lactamases (MBLs), a sub-class of β-lactamases, have been found in several bacteria since they confer resistance to many different β-lactams, and as a result, the bacteria survive in their presence. A potential treatment is to synthesize inhibitors of MBLs, however, there are no inhibitors synthesized in clinical use. The goal of this project …

High Level Learning Using The Temporal Features Of Human Demonstrated Sequential Tasks, Madison Brandywine Clark-Turner

Doctoral Dissertations

Modelling human-led demonstrations of high-level sequential tasks is fundamental to a number of practical inference applications including vision-based policy learning and activity recognition. Demonstrations of these tasks are captured as videos with long durations and similar spatial contents. Learning from this data is challenging since inference cannot be conducted solely on spatial feature presence and must instead consider how spatial features play out across time. To be successful these temporal representations must generalize to variations in the duration of activities and be able to capture relationships between events expressed across the scale of an entire video.

Contemporary deep learning architectures …

Molecular Architecture Of Rod Photoreceptor Phosphodiesterase In Its Nonactivated And G-Protein Activated States, Michael Irwin

Doctoral Dissertations

The photoreceptor phosphodiesterase (PDE6) plays an important role in the G-protein coupled visual signaling pathway which uses cGMP as a second messenger to convert light stimuli into electrical signals. PDE6 is a tetrameric peripheral membrane protein consisting of two catalytic subunits and two inhibitory subunits and is localized to the outer segment membranes of rod and cone photoreceptors. Mutations in this enzyme are one cause of retinitis pigmentosa and other retinal degenerative diseases resulting in blindness or visual dysfunction that lack adequate therapeutic intervention due to inadequate knowledge of PDE6 structure and regulation PDE6 is tightly regulated in the nonactivated …

Biologically Inspired Computer Vision/ Applications Of Computational Models Of Primate Visual Systems In Computer Vision And Image Processing, Reza Hojjaty Saeedy

Doctoral Dissertations

Biologically Inspired Computer VisionApplications of Computational Models of Primate Visual Systems in Computer Vision and Image Processing Reza Hojjaty Saeedy Abstract Biological vision systems are remarkable at extracting and analyzing the information that is essential for vital functional needs. They perform all these tasks with both high sensitivity and strong reliability. They can efficiently and quickly solve most of the difficult computa- tional problems that are still challenging for artificial systems, such as scene segmentation, 3D/depth perception, motion recognition, etc. So it is no surprise that biological vision systems have been a source of inspiration for computer vision problems. In …

The Effect Of Deep-Water Multibeam Mapping Activity On The Foraging Behavior Of Cuvier’S Beaked Whales And The Marine Acoustic Environment, Hilary Suzanne Curran Kates Varghese

Doctoral Dissertations

Sound can propagate great distances underwater and is an important mode for marine life to obtain information. Human activities in the ocean such as global shipping, coastal construction, gas and oil exploration, and mapping navigation routes intentionally and unintentionally emit sound into the ocean, potentially interacting with marine life. Therefore, it is essential that the effects of anthropogenic noise on marine life and the ambient marine acoustic environment be understood. Most of the work, to date, has focused on the impact of low-frequency (<1 kHz) sources such as shipping noise, which is ubiquitous in the ocean, and mid-frequency (1-10 kHz) sources such as naval sonar, to which many marine mammals have shown to be sensitive. The effect of these sources can be as salient as a mass stranding event or as benign as an animal swimming away from a source of noise with no other effect. Less work has focused on higher frequency sources (>10 kHz), including ocean-mapping sonar systems. However, most marine mammals, namely toothed whales (odontocetes), are capable …

Ligand Design In C-Functionalized Cobalt Cyclams For Hybrid Photocatalytic Co2 Reduction, Ethan Edward Jarvis

Doctoral Dissertations

Global climate change is one of the most significant risks to both the planet and all life on it. The largest contributor to this ever-growing threat is CO2 and its continued emission into the atmosphere. As a result, many scientists are researching CO2 reduction processes to help slow and potentially reverse the climate change by reducing the atmospheric levels of CO2. Ideally, these reduction processes convert CO2 into useful materials such as hydrocarbon-based fuels such as methanol or ethanol, which could also help remove our dependence on finite natural energy resources. CO2 reduction is challenging to do efficiently however, due …

Feedback Control Of Fed-Batch Cultures Using Raman Spectroscopy For Metabolite Estimation, Brian Hadley

Master's Theses and Capstones

The monitoring and control of mammalian cell culture processes are critical for the safe and cost-effective development of biotherapeutics in drug development. Current control using off line sampling is infrequent and can miss important metabolic shifts which can impact product quality and consistency. Raman spectroscopy can provide in-line monitoring of multiple metabolites and critical process parameters to allow automation and process improvements. In this research a generic model was developed for monitoring multiple cell lines with a platform Chinese hamster ovary (CHO) cell process. Parameter estimation was then used for the implementation of feedback control. Two nutrient feeds were added …

Post-Liquefaction Residual Strength Of Saturated And Partially Saturated Soils, Ian Douglas Gates

Master's Theses and Capstones

Post-liquefaction response and residual strength play important roles in stability assessment of liquefied ground. Considering the recent advancements in application of induced partial saturation for liquefaction mitigation, the state of knowledge in estimating the residual strength should be extended for liquefied desaturated soils. In this thesis, the residual strength response of a clean sand at different saturation levels was investigated using a Ring Shear Device (RSD). Direct air injection was used to desaturate the soil, which helped mitigating the liquefaction under cyclic loading. However, by raising the shear strain level, both saturated and partially saturated soils were liquefied followed by …

Improving Employee Mental Health: Addressing Depression At The Workplace, Gerald G. Chia Dr.

DNP Scholarly Projects

Abstract Background: Depression is a serious mental health challenge in the US. As frontline workers who are responsible for taking care of a myriad of patients daily, health workers are usually exposed to depressive situations which eventually results in them developing the mental health condition. The purpose of the current project was, therefore, to develop an intervention which helps to improve employee mental health in healthcare settings with focus on depression and examine workplace factors which influence depression among employees of the Outpatient Mental Health Clinic in Washington District of Columbia. Methods: A pre-intervention survey was conducted among 43 employees. …

Estimated Glomerular Filtration Rate And The Racial Multiplier: Clinical Implications And Current Attitudes, Brittney A. Spooner

Honors Theses and Capstones

Chronic kidney disease (CKD) is associated with high morbidity and mortality and has high and increasing incidence worldwide. CKD is characterized by a reduction in glomerular filtration rate (GFR), but as GFR is difficult to measure directly, estimated glomerular filtration rate (eGFR) equations have been created to measure serum creatinine levels as a function of GFR. Some eGFR equations contain a racial multiplier that increases the eGFR of black patients, causing a spurious increase in reported kidney function. This study included a literature search that collected information on the rationale behind the multipliers usage and a survey that gathered information …

Care Ethics, Disability, And Public Policy, Piper Gibson

Honors Theses and Capstones

No abstract provided.

Stressful Life Events And Postpartum Depression, Madison E. Laurent

Honors Theses and Capstones

No abstract provided.

Roles Of Non-Frankia Bacteria In Root Nodule Formation And Function In Alnus Sp., Kelsey Christine Mercurio

Honors Theses and Capstones

Plant roots are home to a wide variety of beneficial microbes; understanding and optimizing plant-microbe interactions may be critical to enhance global food security in a sustainable, equitable way. With the help of their nitrogen-fixing bacterial partner, Frankia, actinorhizal plants form symbiotic root nodules and play important roles in agroforestry and land reclamation. However, Frankia does not live alone in nodules, and the other microbial residents may contribute to nodule formation and function. We collected root nodules from alder trees (Alnus sp.) in 2018 and 2019, then isolated DNA and individual bacterial strains to characterize the nodule microbial …

Sars-Cov-2: An Investigation On Mutagenicity And Its Effects On Infectivity And Mortality, Tyler Elliott Silverwood

Honors Theses and Capstones

SARS-CoV-2, the etiological agent of the COVID-19 pandemic, has rapidly become a worldwide public health concern. Classified as a betacoronavirus, it is the third human coronavirus (HCoV) to emerge in the 21st century that causes severe disease, alongside SARS-CoV and MERS-CoV. The genome consists of open reading frames encoding accessory proteins and four structural proteins, including the spike protein which is a key determinant of host cell tropism. Mutations within the genome, particularly the spike gene, have been linked in-vitro to increased binding affinity to the human receptor angiotensin-converting enzyme 2 (hACE2), increased fitness in human hosts, and immune evasion. …

Multimedia Of The Mind: Digital Rhetoric And Interdisciplinary Acquisition, Parker C. Ayotte

Honors Theses and Capstones

Multimodal digital narratives are currently in the spotlight for acquisition into the digital humanities. The narrative form is emerging with great research interest, the form having no previously established traditions. This research paper attempts to define the nature of multimodal digital narratives and their implementation into modern society. Specifically, the paper addresses the digital rhetoric appropriated by multimodal digital narratives and how it translates into modifying perceptions of society. This multimodal digital rhetoric is then explored in the context of digital activism and education, formative social discourses the augment societal perceptions. Digital rhetoric is utilized to augment a user’s reality …

Resource Availability Drives Patterns Of Productivity Rather Than Biodiversity For Local-Scale Temperate Forests, Kaitlyn Ann Baillargeon

Master's Theses and Capstones

In short-lived, grassland ecosystems, biodiversity and productivity have been found to be positively correlated. However, studies in long-lived, forested ecosystems have found mixed results. Biodiversity in forests has primarily been measured using indices of species diversity, but recent studies have shown that biodiversity is more complex than simply quantifying the number of species occupying an area. Biodiversity can also be represented by other measures of diversity such as functional, phylogenetic, and structural diversity; all three of which have been shown to be more strongly correlated to productivity than species diversity alone. Here, we examined the relationship between four biodiversity measures …

Townhouse Pond 2021 Water Quality Highlight Report, Amanda Mcquaid, Robert Craycraft

UNH Cooperative Extension

A Water Quality Highlight Report by UNH CE Lakes Lay Monitoring Program for Townhouse Pond (of Milton Three Ponds) in Milton, NH in 2021.