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Yes-Associated Protein 1 Is Required For Proliferation And Function Of Bovine Granulosa Cells In Vitro, Michele R. Plewes, Xiaoying Hou, Pan Zhang, Aixin Liang, Guohua Hua, Jennifer R. Wood, Andrea S. Cupp, Xiangmin Lv, Cheng Wang, John S. Davis

Department of Animal Science: Faculty Publications

Yes-associated protein 1 (YAP1) is a major component of the Hippo signaling pathway. Although the exact extracellular signals that control the Hippo pathway are currently unknown, increasing evidence supports a critical role for the Hippo pathway in embryonic development, regulation of organ size, and carcinogenesis. Granulosa cells (GCs) within the ovarian follicle proliferate and produce steroids and growth factors, which facilitate the growth of follicle and maturation of the oocyte.We hypothesize that YAP1 plays a role in proliferation and estrogen secretion of GCs. In the current study, we examined the expression of the Hippo signaling pathway in bovine ovaries and …

Postnatal Β2 Adrenergic Treatment Improves Insulin Sensitivity In Lambs With Iugr But Not Persistent Defects In Pancreatic Islets Or Skeletal Muscle, Dustin T. Yates, Leticia E. Camacho, Amy C. Kelly, Leah V. Steyn, Melissa A. Davis, Andrew T. Antolic, Miranda J. Anderson, Ravi Goyal, Ronald E. Allen, Klearchos K. Papas, William W. Hay Jr, Sean W. Limesand

Department of Animal Science: Faculty Publications

Placental insufficiency causes intrauterine growth restriction (IUGR) and disturbances in glucose homeostasis with associated β adrenergic receptor (ADRβ) desensitization. Our objectives were to measure insulin-sensitive glucose metabolism in neonatal lambs with IUGR and to determine whether daily treatment with ADRβ2 agonist and ADRβ1/β3 antagonists for 1 month normalizes their glucose metabolism. Growth, glucose-stimulated insulin secretion (GSIS) and glucose utilization rates (GURs) were measured in control lambs, IUGR lambs and IUGR lambs treated with adrenergic receptor modifiers: clenbuterol atenolol and SR59230A (IUGR-AR). In IUGR lambs, islet insulin content and GSIS were less than …

The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote

Department of Animal Science: Faculty Publications

Ultrasonography was used to capture a coronal–sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal‐line boars at approximately 6 months of age at the University of Nebraska—Lincoln. Varicocoele was diagnosed by two methods. Method 1 diagnosed varicocoele when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocoele when the average vessel area on one side of the scrotum of a boar was 1.5 times larger than the average vessel on the …

Adaptation Strategies: Ruminants, John B. Gaughan, Veerasamy Sejian, Terry L. Mader, Frank R. Dunshea

Department of Animal Science: Faculty Publications

• Growing populations and reduced access to arable land mean that animal production systems will either need to intensify and/or produce more from a reducing land and other resource base.

• Variable and unpredictable environmental conditions mean that animal production faces numerous challenges. In add- ition to climate, these challenges include increased disease risk, increased nutritional deficiencies, and lack of capital to support diversification.

• Predicted changes in climate will impose selection pressures on traits important for biological fitness (and production).

• Genetic adaptation is important for the future of livestock systems. Animal adaptation involves trade-offs, which must be considered when selecting animals for …

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Livestock Animals To Study Infertility In Women, Mohamed A. Abedal-Majed, Andrea S. Cupp

Department of Animal Science: Faculty Publications

• Anovulation is a major cause of infertility in mammalian females.

• Ovarian research in humans is challenging due to the shortage of “normal tissue” that can be obtained from women that are trying to get pregnant.

• Livestock animals provide valuable resources to investigate the optimal intra-follicular environment required to facilitate folliculogenesis and translate to humans.

• Different livestock models may be more useful depending on the specific biological process studied.

Changes In North American Mammal Niche Preferences From The Late Pleistocene To The Present, Silvia Pineda-Munoz, Anikó Tóth, S. Kathleen Lyons, Yue Wang, Jenny Mcguire

School of Biological Sciences: Presentations

Human population has exponentially grown since the last glaciation, especially across temperate areas with easy access to water sources, excluding mammal species from their former habitats. Thus, we anticipate a change in environmental niche preferences for temperature and precipitation as increased human population forces mammal species into more extreme climates within their environmental tolerances. For our study, we collected species occurrences from 20,000 ybp to the present for 59 North American mammal species. We inferred temperature and precipitation for each location using paleoclimate simulations (CCSM3). Overall, we found that mammals now live in areas that are warmer and dryer on …

Handling George Eliot’S Fiction, Peter J. Capuano

Department of English: Faculty Publications

An argument that George Eliot was a novelist intellectually, philosophically, and aesthetically ahead of the majority of her peers thankfully needs no defense two hundred years after her birth. This lofty status, however, does not mean that Eliot was impervious to the cultural preoccupations of her time. Quite the contrary. A central contention of this essay is that Eliot, despite her imposing intellectual reputation, engaged with her culture’s popular interest in human hands in ways that profoundly affected her fiction. As I have argued elsewhere,¹ the Victorians became highly cognizant of the physicality of their hands in large part …

Companion Animal Ectoparasites, Jennifer Furchak

Department of Entomology: Distance Master of Science Projects

Fleas 3

Ticks 6 Brown Dog Tick 7 American Dog Tick 8 Black-legged Tick 9 Lone Star Tick 10 Gulf Coast Tick 11

Mosquitoes 13

Parasite Comparison 15

Control and Prevention 16

Active Ingredients 19

Integrated Pest Management 20

Resources for Pet Parents 21

References 22

Lesson Plan: Bug Basics, Kelsey Larsen

Department of Entomology: Distance Master of Science Projects

Grades: Pre-K & Kindergarten

Time Frame of Instruction: 40-45 minutes

What Skill Are Being Taught? Through hands-on and listening activities, student should be able to: Identify common insects by sight and sound. Recognize difference between an insect and other bugs such as spiders, centipedes, etc. - Differences should include but not limited to the number of body parts & legs (main way to distinguish between an insect, spider, etc.)

Educational Components of Classroom Project: 1. Science: Students will learn the basics about insect biology and how to identify common “bugs” 2. Visual: Students will be able handle plastic bugs in …

Building An Integrated Pest Management Plan For A Unique Urban Farming Program, Jeff Coco

Department of Entomology: Distance Master of Science Projects

The summer of 2018 led to an opportunity with a local non-profit called Fleet Farming. This non-profit had different departments available for internships which included greenhouse and farming. I was familiar with the program in that I knew they farmed in people’s front yards, in ‘farmlettes’ that had been converted from lawns. I was most interested in the job description under farming that said “practicing IPM”. I had developed an interest in IPM when I took Dr. Tom Weissling’s class on the Management of horticultural crop insects in the Spring of 2018. I wanted more ‘hands on’ learning and this …

Size Estimation Of Pre‐Columbian Caribbean Fish, Sandrine Grouard, Sophia Perdikaris, Nídia Cristina Espíndola Rodrigues, Irvy R. Quitmyer

School of Global Integrative Studies: Faculty Publications

In this contribution, we present a methodological approach to the identification of pre‐Columbian Caribbean fisheries and examine the interrelationships of exploitation according to size for eight fish families, in a diachronic perspective for the Lesser Antilles. Based on the principles of size and growth allometries, biometric repositories have been reconstructed for modern families that represent different ecological environments: Holocentridae, Serranidae, Carangidae, Lutjanidae, Haemulidae, Scaridae, Acanthuridae, and Scombridae. The measured fish bone elements were selected based on their robustness and potential for recovery at archaeological sites. This resulted in a sample size totaling 563 modern osteological specimens, which provided reconstructed standard, …

Wpa News 110 (2019), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Winter 2019), number 110

Published by the World Pheasant Association

Outcomes Of The Iowa Parent Partner Program Evaluation: Stability Of Reunification And Re-Entry Into Foster Care, Jeff M. Chambers, Sandy Lint, Maggie G. Thompson, Matthew W. Carlson, Michelle Graef

Center on Children, Families, and the Law: Faculty Publications

In an effort to facilitate family engagement with services, improve reunification outcomes, and empower the families they serve, child welfare agencies across the country have developed and implemented programs designed to provide peer mentoring. These programs work to identify parents who have successfully navigated the child welfare system in the past and train them to mentor parents who are currently in the system. The current study used a quasi-experimental design and propensity score matching to examine the outcomes for children of families served by the Iowa Department of Human Services Parent Partner program, one of the earliest and most established …

Economic Analysis Of Deficit Irrigation In Sugarcane Farming: Nchalo Estate, Chikwawa District, Malawi, Mavuto M. Banda, Derek M. Heeren, Derrel Martin, Francisco Muñoz-Arriola, László G. Hayde

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Sustenance of the growing world population calls for increased agricultural production. However, this will have to be done while forecasts of water withdrawals on a global scale predict sharp increases in future demand to meet human needs. The inadequacy of irrigation water supplies has led to the need to consider deficit irrigation (DI) as a water saving strategy. DI is a deliberate under-application of water to growing crops.

In this study we carried out an economic analysis of DI in sugarcane farming with an aim of developing an understanding of the economic impact of various irrigation water management strategies. The …

Patellar Tendon Morphology In Trans-Tibial Amputees Utilizing A Prosthesis With A Patellar-Tendonbearing Feature, Kai-Yu Ho, Michelle Harty, Jessica Kellogg, Kelly Teter, Szu-Ping Lee, Yu-Jen Chang, Gregory Bashford

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A patellar-tendon-bearing (PTB) bar is a common design feature used in the socket of trans-tibial prostheses to place load on the pressure-tolerant tissue. As the patellar tendon in the residual limb is subjected to the perpendicular compressive force not commonly experienced in normal tendons, it is possible for tendon degeneration to occur over time. The purpose of this study was to compare patellar tendon morphology and neovascularity between the residual and intact limbs in trans-tibial amputees and healthy controls. Fifteen unilateral trans-tibial amputees who utilized a prosthesis with a PTB feature and 15 age- and sex- matched controls participated. Sonography …

A Hierarchical Bayesian Approach For Handling Missing Classification Data, Alison C. Ketz, Therese L. Johnson, Mevin B. Hooten, N. Thompson Hobbs

United States National Park Service: Publications

1. Ecologists use classifications of individuals in categories to understand composition of populations and communities. These categories might be defined by demo- graphics, functional traits, or species. Assignment of categories is often imperfect, but frequently treated as observations without error. When individuals are observed but not classified, these “partial” observations must be modified to include the missing data mechanism to avoid spurious inference.

2. We developed two hierarchical Bayesian models to overcome the assumption of perfect assignment to mutually exclusive categories in the multinomial distribu- tion of categorical counts, when classifications are missing. These models incorporate auxiliary information to adjust the posterior …

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Spatial Conservation Planning Under Uncertainty: Adapting To Climate Change Risks Using Modern Portfolio Theory, Mitchell J. Eaton, Simeon Yurek, Zulqarnain Haider, Julien Martin, Fred A. Johnson, Bradley J. Udell, Hadi Charkhgard, Changhyun Kwon

United States Geological Survey: Staff Publications

Climate change and urban growth impact habitats, species, and ecosystem services. To buffer against global change, an established adaptation strategy is designing protected areas to increase representation and complementarity of biodiversity features. Uncertainty regarding the scale and magnitude of landscape change complicates reserve planning and exposes decision makers to the risk of failing to meet conservation goals. Conservation planning tends to treat risk as an absolute measure, ignoring the context of the management problem and risk preferences of stakeholders. Application of risk management theory to conservation emphasizes the diversification of a portfolio of assets, with the goal of reducing the …

Urease Is An Essential Component Of The Acid Response Network Of Staphylococcus Aureus And Is Required For A Persistent Murine Kidney Infection, Chunyi Zhou, Fatema Bhinderwala, Mckenzie K. Lehman, Vinai C. Thomas, Sujata S. Chaudhari, Kelsey J. Yamada, Kirk W. Foster, Robert Powers, Tammy Kielian, Paul D. Fey

Department of Chemistry: Faculty Publications

Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH₃ and CO₂ from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation. However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied. We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes …

Longitudinal Study Of Humoral Immunity To Bovine Coronavirus, Virus Shedding, And Treatment For Bovine Respiratory Disease In Pre-Weaned Beef Calves, Aspen M. Workman, Larry A. Kuehn, Tara G. Mcdaneld, Michael L. Clawson, John Dustin Loy

Department of Animal Science: Faculty Publications

Background: Bovine coronavirus (BCV) is associated with respiratory infections in cattle of all ages; however, a temporal study to evaluate the effect of BCV immunity on virus shedding and bovine respiratory disease (BRD) incidence in pre-weaned beef calves has not been reported. Thus, we report here a prospective study in three herds of crossbred beef calves (n = 817) with endemic BCV. Serial blood samples for measurement of serum anti-BCV antibody titers and nasal swabs for detection of BCV and other common viral and bacterial BRD pathogens were collected from all calves or subsets of calves at predetermined times …

The Impact Of Heat Load On Cattle, Angela M. Lees, Veerasamy Sejian, Andrea L. Wallage, Cameron C. Steel, Terry L. Mader, Jarrod C. Lees, John B. Gaughan

Department of Animal Science: Faculty Publications

Heat stress and cold stress have a negative influence on cattle welfare and productivity. There have been some studies investigating the influence of cold stress on cattle, however the emphasis within this review is the influence of heat stress on cattle. The impact of hot weather on cattle is of increasing importance due to the changing global environment. Heat stress is a worldwide phenomenon that is associated with reduced animal productivity and welfare, particularly during the summer months. Animal responses to their thermal environment are extremely varied, however, it is clear that the thermal environment influences the health, productivity, and …

Reducing Methane Production With Corn Oil And Calcium Sulfate: Responses On Whole-Animal Energy And Nitrogen Balance In Dairy Cattle, J. V. Judy, G. C. Bachman, T. M. Brown-Brandl, S. C. Fernando, K. E. Hales, P. S. Miller, R. R. Stowell, P. J. Kononoff

Department of Animal Science: Faculty Publications

The addition of fat and calcium sulfate to diets fed to ruminants has resulted in a reduction in methane production, but the effects on energy balance have not been studied. A study using indirect calorimetry and 16 multiparous (8 Holstein and 8 Jersey; 78 ± 15 d in milk; mean ± standard deviation) lactating dairy cows was conducted to determine how mitigating methane production by adding corn oil or calcium sulfate to diets containing reduced-fat distillers grains affects energy and nitrogen balance. A replicated 4 × 4 Latin square design with 35-d periods (28 d of adaption and 4 d …

Real Supermodels Wear Wool: Summarizing The Impact Of The Pregnant Sheep As An Animal Model For Adaptive Fetal Programming, Kristin A. Beede, Sean W. Limesand, Jessica L. Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

• Intrauterine growth restriction (IUGR) continues to be a global epidemic that is associated with high early-life mortality rates and greater risk for developing metabolic disorders that lower length and quality of life in affected individuals.

• Fetal programming of muscle growth and metabolic function associated with IUGR is often comparable among nonlitter bearing mammalian species, which allows much of the information learned in domestic animal models to be applicable to humans (and other animals).

• Recent studies in sheep models of IUGR have begun to uncover the molecular mechanisms linking adaptive fetal programming and metabolic dysfunction.

• Targets of …

Perturbations In Lineage Specification Of Granulosa And Theca Cells May Alter Corpus Luteum Formation And Function, Mohamed A. Abedel-Majed, Sarah M. Romereim, John S. Davis, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Anovulation is a major cause of infertility, and it is the major leading reproductive disorder in mammalian females. Without ovulation, an oocyte is not released from the ovarian follicle to be fertilized and a corpus luteum is not formed. The corpus luteum formed from the luteinized somatic follicular cells following ovulation, vasculature cells, and immune cells is critical for progesterone production and maintenance of pregnancy. Follicular theca cells differentiate into small luteal cells (SLCs) that produce progesterone in response to luteinizing hormone (LH), and granulosa cells luteinize to become large luteal cells (LLCs) that have a high rate of basal …

Effect Of Harvest Method And Ammoniation Of Baled Corn Residue On Intake And Digestibility In Lambs, Ashley C. Conway, Tasha M. King, Melissa L. Jolly, J. C. Macdonald, Terry J. Klopfenstein, Mary E. Drewnoski

Department of Animal Science: Faculty Publications

To determine the effect of harvest method and ammoniation on both in vivo and in vitro digestibility of corn residue, six corn residue treatments consisting of three different harvest methods either with or without anhydrous ammonia chemical treatment (5.5% of dry matter [DM]) were evaluated. The harvest methods included conventional rake-and-bale (CONV) and New Holland Cornrower with eight rows (8ROW) or two rows (2ROW) of corn stalks chopped into the windrow containing the tailings (leaf, husk, and upper stem) from eight rows of harvested corn (ammoniated bales of each harvest method resulted in treatments COVAM, 8RAM, and 2RAM). Nine crossbred …

Nres/Bsen 468/868: Wetlands, Tiffany L. Messer

UNL Faculty Course Portfolios

Wetlands (468/868) is a multidisciplinary 4 hr fourth year/graduate level course that includes two 90 min lectures and a weekly 3 hr laboratory. The course is crosslisted in the School of Natural Resources and Biological Systems Engineering Department. The course is typically comprised of 20-30 students with approximately 30% engineering students and 70% natural resources students in the majors of biological systems engineering, water science, fisheries and wildlife, and environmental restoration science. Majority of the students that participate in this course intend to have careers in consulting, government, and/or the nonprofit conservation sectors. Each of these sectors require knowledge of …

Nutr455: Advanced Nutrition And Human Metabolism- A Peer Review Of Teaching Project Benchmark Portfolio, Sathish Kumar Natarajan

UNL Faculty Course Portfolios

NUTR455, Advanced Nutrition and Human Metabolism is a capstone, ACE 10 course for undergraduate seniors in nutrition and health sciences that I teach in spring semesters since 2017. Among the 3 classes that I taught, spring 2019 class did awesome job in their overall performance, in-class participation, and in-class exams and in their final poster assignment. We analyzed three questions to as part of this teaching benchmark portfolio. 1) The role of in-class iClicker questions in improving student students understanding the key concepts and test whether it motivates the students to study course materials; 2) Tested whether review session impacted/improve …

Peer Review Of Teaching Portfolio: Psychology Of Social Behavior (Psyc 288), Ashley M. Votruba

UNL Faculty Course Portfolios

This course portfolio assesses student learning in the Psychology of Social Behavior course, PSYC 288. This course introduces students to the field of social psychology—the scientific study of the way in which people’s thoughts, feelings, and behaviors are influenced by the real or imagined presence of other people. This course covers the fundamental principles, theories, methods, experiments, and people of social psychology. Student enrollment for this course consists of 200 to 250 undergraduates from a variety of majors with diverse educational backgrounds and familiarity with social psychology. The course portfolio outlines the goals, learning outcomes, and assessments for this course. …

Птицы Восточного Саяна / Birds Of The Eastern Sayan, Tsydypzhap Zayateevich Dorzhiev, Yuri Anatolyevich Durnev, Marina Vitalievna Sonina, Erdeni Nikolaevich Elaev, A. A. Baranov

Zea E-Books Collection

The monograph presents data on the distribution and ecology of 340 species of birds found on the territory of the poorly studied highland — the Eastern Sayan. An ecological systematic and faunogenetic analysis of the region’s avifauna has been carried out. We reveal some features of the birds' way of life in the extreme natural conditions of the mountains of Southern Siberia.

The book is intended for all who are of interest in the wildlife of Siberia, as well as for biology teachers and students, ecologists.

В монографии приведены данные о распространении и экологии 340 видов птиц, отмеченных на территории …

Radiomic Feature Stability Across 4d Respiratory Phases And Its Impact On Lung Tumor Prognosis Prediction, Qian Du, Michael Baine, Kyle Bavitz, Josiah Mcallister, Xiaoying Liang, Hongfeng Yu, Jeffrey Ryckman, Lina Yu, Hengle Jiang, Sumin Zhou, Sumin Zhou, Chi Zhang, Dandan Zheng

School of Biological Sciences: Faculty Publications

Radiomic analysis has recently demonstrated versatile uses in improving diagnostic and prognostic prediction accuracy for lung cancer. However, since lung tumors are subject to substantial motion due to respiration, the stability of radiomic features over the respiratory cycle of the patient needs to be investigated to better evaluate the robustness of the interpatient feature variability for clinical applications, and its impact in such applications needs to be assessed. A full panel of 841 radiomic features, including tumor intensity, shape, texture, and wavelet features, were extracted from individual phases of a four-dimensional (4D) computed tomography on 20 early-stage non-small-cell lung cancer …

Arena Size Modulates Functional Responses Via Behavioral Mechanisms, Stella F. Uiterwaal, Anthony I. Dell, John P. Delong

School of Biological Sciences: Faculty Publications

Laboratory-based functional response experiments, in which foraging rates are measured across a range of resource densities, are central for determining trophic interaction strength. Historically these experiments often are performed in arbitrarily sized arenas, with larger sized organisms generally used in larger arenas. However, arena size influences foraging rates and therefore also estimates of the functional response parameters, particularly space clearance rate (attack rate). We hypothesized that nonrandom movement within arenas by predators and prey may explain this effect. To test this hypothesis, we video-recorded Schizocosa ocreata wolf spiders (predators) and flightless Drosophila melanogaster prey in circular arenas of 3 different …