Digital Commons Network^™

Ligand Binding And Signaling Of Hare/Stabilin-2, Edward N. Harris, Fatima Cabral

Department of Biochemistry: Faculty Publications

The Stabilin receptors are a two-member family in the type H class of scavenger receptors. These dynamic receptors bind and internalize multiple ligands from the cell surface for the purpose of clearing extracellular material including some synthetic drugs and for sensing the external environment of the cell. Stabilin-1 was the first receptor to be cloned, though the biological activity of Hyaluronic Acid Receptor for Endocytosis (HARE)/Stabilin-2 was observed about 10 years prior to the cloning of Stabilin-1. Stabilin-1 has a more diverse expression profile among the tissues than HARE/Stabilin-2. This review will focus on HARE/Stabilin-2 and its interactions with hyaluronan, …

Genome‐Wide Association And Genomic Prediction For Biomass Yield In A Genetically Diverse Miscanthus Sinensis Germplasm Panel Phenotyped At Five Locations In Asia And North America, Lindsay V. Clark, Maria S. Dwiyanti, Kossonou G. Anzoua, Joe E. Brummer, Bimal Kumar Ghimire, Katarzyna Glowacka, Megan Hall, Kweon Heo, Xiaoli Jin, Alexander E. Lipka, Junhua Peng, Toshihiko Yamada, Ji Hye Yoo, Chang Yeon Yu, Hua Zhao, Stephen P. Long, Erik J. Sacks

Department of Biochemistry: Faculty Publications

To improve the efficiency of breeding of Miscanthus for biomass yield, there is a need to develop genomics‐assisted selection for this long‐lived perennial crop by relating genotype to phenotype and breeding value across a broad range of environments. We present the first genome‐wide association (GWA) and genomic prediction study of Miscanthus that utilizes multilocation phenotypic data. A panel of 568 Miscanthus sinensis accessions was genotyped with 46,177 single nucleotide polymorphisms (SNPs) and evaluated at one subtropical and five temperate locations over 3 years for biomass yield and 14 yield‐component traits. GWA and genomic prediction were performed separately for different years …

Downregulation Of A Cyp74 Rubber Particle Protein Increases Natural Rubber Production In Parthenium Argentatum, Dante F. Placido, Niu Dong, Chen Dong, Von Mark V. Cruz, David A. Dierig, Rebecca E. Cahoon, Byung-Guk Kang, Trinh Huynh, Maureen Whalen, Grisel Ponciano, Colleen Mcmahan

Department of Biochemistry: Faculty Publications

We report functional genomics studies of a CYP74 rubber particle protein from Parthenium argentatum, commonly called guayule. Previously identified as an allene oxide synthase (AOS), this CYP74 constitutes the most abundant protein found in guayule rubber particles. Transgenic guayule lines with AOS gene expression down-regulated by RNAi (AOSi) exhibited strong phenotypes that included agricultural traits conducive to enhancing rubber yield. AOSi lines had higher leaf and stem biomass, thicker stembark tissues, increased stem branching and improved net photosynthetic rate. Importantly, the rubber content was significantly increased in AOSi lines compared to the wild-type (WT), vector control and …

Chloroviruses Lure Hosts Through Long-Distance Chemical Signaling, David Dunigan, Maitham Ahmed Al-Sammak, Zeina Al-Ameeli, Irina Agarkova, John Delong, James L. Van Etten

James Van Etten Publications

Chloroviruses exist in aquatic systems around the planet where they infect certain eukaryotic green algae that are mutualistic endosymbionts in a variety of protists and metazoans. Natural chlorovirus populations are seasonally dynamic but the precise temporal changes in these populations and the mechanisms that underlie them have, heretofore, been unclear. We recently reported the novel concept that predator/prey-mediated virus activation regulates chlorovirus population dynamics, and in the current manuscript demonstrate virus packaged chemotactic modulation of prey behavior.

Viruses have not previously been reported to act as chemotactic/chemo-attractive agents. Rather, viruses as extracellular entities are generally viewed as non-metabolically active spore-like …

Longitudinal Study Of Humoral Immunity To Bovine Coronavirus, Virus Shedding, And Treatment For Bovine Respiratory Disease In Pre-Weaned Beef Calves, Aspen M. Workman, Larry A. Kuehn, Tara G. Mcdaneld, Michael L. Clawson, John Dustin Loy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Bovine coronavirus (BCV) is associated with respiratory infections in cattle of all ages; however, a temporal study to evaluate the effect of BCV immunity on virus shedding and bovine respiratory disease (BRD) incidence in pre-weaned beef calves has not been reported. Thus, we report here a prospective study in three herds of crossbred beef calves (n = 817) with endemic BCV. Serial blood samples for measurement of serum anti-BCV antibody titers and nasal swabs for detection of BCV and other common viral and bacterial BRD pathogens were collected from all calves or subsets of calves at predetermined …

Wheat Streak Mosaic Virus P1 Binds To Dsrnas Without Size And Sequence Specificity And A Gw Motif Is Crucial For Suppression Of Rna Silencing, Adarsh K. Gupta, Satyanarayana Tatineni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus; family Potyviridae) is an economically important virus infecting wheat in the Great Plains region of the USA. Previously, we reported that the P1 protein of WSMV acts as a viral suppressor of RNA silencing. In this study, we delineated the minimal region of WSMV P1 and examined its mechanisms in suppression of RNA silencing. We found that the 25 N-terminal amino acids are dispensable, while deletion of a single amino acid at the C-terminal region completely abolished the RNA silencing suppression activity of P1. Electrophoretic mobility shift assays with in vitro …

The Role Of Genotype And Production Environment In Determining The Cooking Time Of Dry Beans (Phaseolus Vulgaris L.), Karen A. Cichy, Jason A. Wiesinger, Matthew Berry, Susan Nchimbi‐Msolla, Deidre Fourie, Timothy G. Perch, Daniel Ambechew, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dry bean (Phaseolus vulgaris L.) is a nutrient‐dense food rich in proteins and minerals. Although a dietary staple in numerous regions, including Eastern and Southern Africa, greater utilization is limited by its long cooking time as compared with other staple foods. A fivefold genetic variability for cooking time has been identified for P. vulgaris, and to effectively incorporate the cooking time trait into bean breeding programs, knowledge of how genotypes behave across diverse environments is essential. Fourteen bean genotypes selected from market classes important to global consumers (yellow, cranberry, light red kidney, red mottled, and brown) were grown …

Capacity To Elicit Cytotoxic Cd8 T Cell Activity Against Mycobacterium Avium Subsp. Paratuberculosis Is Retained In A Vaccine Candidate 35 Kda Peptide Modified For Expression In Mammalian Cells, Valentina Franceschi, Asmaa H. Mahmoud, Gaber S. Abdellrazeq, Giulia Tebaldi, Francesca Macchi, Luca Russo, Lindsay M. Fry, Mahmoud M. Elnaggar, John Bannantine, Kun-Taek Park, Victoria Hulubei, Sandro Cavirani, William C. Davis, Gaetano Donofrio

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Studies focused on development of an attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of paratuberculosis (Ptb) in cattle and other species, revealed that deletion of relA, a global gene regulator, abrogates the ability of Map to establish a persistent infection. In the absence of relA, cattle develop CD8 cytotoxic T cells (CTL) with the ability to kill intracellular bacteria. Analysis of the recall response to a relA mutant, Map/􏰀relA, with cells from a vaccinated steer demonstrated that a 35-kDa membrane peptide (MMP) is one of the targets of the response. This observation suggested that it might …

Seedling Root Architecture And Its Relationship With Seed Yield Across Diverse Environments In Phaseolus Vulgaris, Christopher F. Strock, James Burridge, Anica S.F. Massas, James S. Beaver, Steve E. Beebe, Samuel A. Camilo, Deidre Fourie, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Eninka Mndolwa, Susan Nchimbi-Msolla, Jose Polania, T. Porch, Juan C. Rosas, Jennifer J. Trapp, Jonathan P. Lynch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Seedling root phenotypes may have important impacts on fitness and are more easily measured than mature root phenotypes. We phenotyped the roots of 577 genotypes of common bean (Phaseolus vulgaris), representing the bulk of the genetic diversity for recent cultivars and landraces in this species. Root architectural phenotypes of seedlings germinated for nine days were compared to root architectural phenotypes in the field as well as seed yield across 51 environments with an array of abiotic stresses including drought, nutrient deficiency, and heat, as well as non-stress conditions. We observed repeatability ranging from 0.52–0.57 for measures of root phenotypes in …

Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Macrophages play an important role in the host immune response to Mycobacterium avium subsp. paratuberculosis (MAP) infection, however, MAP is able to disrupt normal macro- phage functions to avoid destruction. It is unclear whether the phenotypes of macrophages present in the target tissue play a role in the inability to clear MAP infection. The aim of this study was to identify macrophage phenotypes (host defense or resolution and repair) present within the bovine ileum of naturally infected cattle, as well as to ascertain abundance of each macrophage phenotype present during different stages of MAP infection. Immunofluo- rescent (IF) labeling was …

Wearable Devices For Single Cell Sensing And Transfection, Lingqian Chang, Yu-Chieh Wang, Faheem Ershad, Ruiguo Yang, Cunjiang Yu, Yubo Fan

Department of Mechanical and Materials Engineering: Faculty Publications

Transdermal drug delivery systems have been successful for decades. Now these devices can be further scaled down, and their applications have been extended to wide selections of cargo, ranging from natural molecules (e.g., insulin and glucose) to bioengineered molecules (e.g., nanoparticles and vaccines). Some emerging nanopatches show promise for precise single-cell gene transfection in vivo and have advantages over conventional tools in terms of delivery efficiency, safety, and controllability of delivered dose. In this review, we discuss recent technical advances in wearable micro/nano devices with unique capabilities or potential for single-cell biosensing and transfection in the skin or other organs, …

Optimization Of Protein-Protein Interaction Measurements For Drug Discovery Using Afm Force Spectroscopy, Yongliang Yang, Bixi Zeng, Zhiyong Sun, Amir Monemianesfahani, Jing Hou, Nian-Dong Jiao, Lianqing Liu, Liangliang Chen, Marc D. Basson, Lixin Dong, Ruiguo Yang, Ning Xi

Department of Mechanical and Materials Engineering: Faculty Publications

Increasingly targeted in drug discovery, protein-protein interactions challenge current high throughput screening technologies in the pharmaceutical industry. Developing an effective and efficient method for screening small molecules or compounds is critical to accelerate the discovery of ligands for enzymes, receptors and other pharmaceutical targets. Here, we report developments of methods to increase the signal-to-noise ratio (SNR) for screening protein-protein interactions using atomic force microscopy (AFM) force spectroscopy. We have demonstrated the effectiveness of these developments on detecting the binding process between focal adhesion kinases (FAK) with protein kinase B (Akt1), which is a target for potential cancer drugs. These developments …

Local Control Robotic Surgical Devices And Related Methods, Eric Markvicka, Tom Frederick, Jack Mondry, Joe Bartels, Shane Michael Farritor

Department of Mechanical and Materials Engineering: Faculty Publications

The various robotic medical devices include robotic devices that are disposed within a body cavity and positioned using a support component disposed through an orifice or opening in the body cavity. Additional embodiments relate to devices having arms coupled to a device body wherein the device has a minimal profile such that the device can be easily inserted through smaller incisions in comparison to other devices without such a small profile. Further embodiments relate to methods of operating the above devices.

Medical Inflation, Attachment And Delivery Devices And Related Methods, Mark Rentschler, Shane Michael Farritor

Department of Mechanical and Materials Engineering: Faculty Publications

The various embodiments disclosed herein relate to procedural space maintenance devices, medical device positioning devices, and devices that provide both procedural space maintenance and device positioning. Further embodiments relate to medical device insertion and/or retraction devices.

Spatial Repellency, Antifeedant Activity And Toxicity Of Three Medium Chain Fatty Acids And Their Methyl Esters Of Coconut Fatty Acid Against Stable Flies, Gwang H. Roh, Xiaaojie Zhou, Yansu Wang, Steven C. Cermak, James A. Kenar, Alexander Lehmann, Baoyu Han, David B. Taylor, Xiaopeng Zeng, Chung Gyoo Park, Gary J. Brewer, J.J. Zhu

Department of Entomology: Faculty Publications

BACKGROUND: Stable flies are one of the most detrimental arthropod pests to livestock. With changing climates and agronomic practices, they expand their roles as pests and disease vectors as well. Their painful bites reduce livestock productivity, annoy companion animals, and interfere with human recreational activities. Current management technologies are unable to effectively control stable flies. The present study reports new results concerning the contact, spatial repellency, and toxicity of a bio-based product, coconut fatty acid and their methyl ester derivatives of free fatty acids of C8:0, C10:0 and C12:0 to stable flies.

RESULTS: Three medium chain fatty acid methyl esters …

The Genera Of Agaocephalini (Coleoptera: Scarabaeidae: Dynastinae) Of Colombia, With Description Of A New Species Of Lycomedes Breme, Jhon César Neita-Moreno, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

The six genera of Agaocephalini in Colombia are distinguished in a dichotomous key and illustrated. Lycomedes enigmaticus Neita-Moreno and Ratcliffe, new species, is described and differentiated by character comparisons with other previously known Colombian species. Lycomedes lydiae Arnaud, 2012 is proposed as a new junior synonym of Lycomedes reichei Brême, 1844.

Los seis géneros de Agaocephalini en Colombia son incluidos e ilustrados en una clave dicotómica. Se describe Lycomedes enigmaticus Neita-Moreno y Ratcliffe, especie nueva y es diferenciada por comparación de caracteres con otras especies previamente conocidas de Colombia. Se propone Lycomedes lydiae Arnaud, 2012 como un nuevo sinónimo …

Bifenthrin Baseline Susceptibility And Evaluation Of Simulated Aerial Applications In Striacosta Albicosta (Lepidoptera: Noctuidae), Débora G. Montezano, Thomas E. Hunt, Dariane Souza, Bruno C. Vieira, Ana M. Velez, Greg Kruger, Sarah N. Zukoff, Jeff D. Bradshaw, Julie A. Peterson

Department of Entomology: Faculty Publications

Striacosta albicosta (Smith) is a maize pest that has recently expanded its geographical range into the eastern United States and southeastern Canada. Aerial application of pyrethroids, such as bifenthrin, has been a major practice adopted to manage this pest. Reports of field failure of pyrethroids have increased since 2013. Striacosta albicosta populations were collected in 2016 and 2017 from maize fields in Nebraska, Kansas, and Canada and screened with bifenthrin active ingredient in larval contact dose-response bioassays. Resistance ratios estimated were generally low in 2016 (1.04- to 1.32-fold) with the highest LC50 in North Platte, NE (66.10 ng/cm²) …

Egg Albumin As A Protein Marker To Study Dispersal Of Noctuidae In The Agroecosystem, C. S. Tavares, Silvana L. Vieira De Paula Moraes, A. Valencia-Jimenez, Thomas E. Hunt, A. M. Vélez, E. J. G. Pereira

Department of Entomology: Faculty Publications

Knowledge of dispersal and spatial dynamics of pest populations is fundamental for implementation of integrated pest management and integrated resistance management.This study evaluated 1) the effectiveness of egg white albumin protein to mark larvae and adults of two polyphagous and highly mobile pests, Spodoptera frugiperda (J.E. Smith) (fall armyworm) and Helicoverpa zea (Boddie) (corn earworm) (Lepidoptera: Noctuidae), and 2) the sensitivity of polyvinylidene difluoride membrane (dot blot) in detecting albumin on marked insects. Laboratory and field experiments tested egg albumin as a protein marker, which was detected using two enzyme-linked immunosorbent assay (ELISA), microplate, and dot blot. In the laboratory, …

Insights Into Gemcitabine Resistance And The Potential For Therapeutic Monitoring, Teklab Gebregiworgis, Fatema Bhinderwala, Vinee Purohit, Nina V. Chaika, Pankaj K. Singh, Robert Powers

Department of Chemistry: Faculty Publications

Introduction—Gemcitabine is an important component of pancreatic cancer clinical management. Unfortunately, acquired gemcitabine resistance is widespread and there are limitations to predicting and monitoring therapeutic outcomes.

Objective—To investigate the potential of metabolomics to differentiate pancreatic cancer cells that develops resistance or respond to gemcitabine treatment.

Results—We applied 1D ¹H and 2D ¹H-¹³C HSQC NMR methods to profile the metabolic signature of pancreatic cancer cells. ¹³C₆-glucose labeling identified thirty key metabolites uniquely altered between wild-type and gemcitabine-resistant cells upon gemcitabine treatment. Gemcitabine resistance was observed to reprogram glucose metabolism and to enhance the pyrimidine …

Detailed Characterization Of The Solution Kinetics And Thermodynamics Of Biotin, Biocytin And Haba Binding To Avidin And Streptavidin, Roberto F. Delgadillo, Timothy C. Mueser, Kathia Zaleta-Rivera, Katie A. Carnes, Jose Gonzalez-Valdez, Lawrence J. Parkhurst

Department of Chemistry: Faculty Publications

The high affinity (K_D ~ 10⁻¹⁵ M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume that the association rate constant (kon) is approximately diffusion limited (10⁹ M^-1s^-1) but recent single molecule and surface binding studies indicate that they are slower than expected (10⁵ to 10⁷ M^-1s^-1). In this study, we asked whether these reactions in solution are diffusion controlled, which reaction model and thermodynamic …

Automatic Nonnutritive Suck Waveform Discrimination And Feature Extraction In Preterm Infants, Chunxiao Liao, Austin O. Rosner, Jill L. Maron, Dongli Song, Steven M. Barlow

Department of Special Education and Communication Disorders: Faculty Publications

Background and Objective: The emergence of the nonnutritive suck (NNS) pattern in preterm infants reflects the integrity of the brain and is used by clinicians in the neonatal intensive care unit (NICU) to assess feeding readiness and oromotor development. A critical need exists for an integrated software platform that provides NNS signal preprocessing, adaptive waveform discrimination, feature detection, and batch processing of big data sets across multiple NICU sites. Thus, the goal was to develop and describe a crossplatform graphical user interface (GUI) and terminal application known as NeoNNS for single and batch file time series and frequency-domain analyses of …

Nebraska's Advantage: Productive Agriculture And Bountiful Natural Resources, Laura E. Young, Mary Garbacz, Michael J. Boehm, Bethany Karlberg, John Carroll, Kathryn Bagniewski, Daran Rudnick, Halle Ramsey, Marc Libault, Cadrien Livingston, Jamilynn Poletto, Leanne Gamet, Troy Gilmore, Natalie Jones, Lisa Pennisi, Elizabeth Vanwormer, Abby Steffen, Andrea Basche, Rylie Kalb, Rick Koelsch, Bethany Karlberg, Martha Shulski, Michael Hayes, Peter Mccornick, Amit Jhala, Walter Schacht, Stanosh Pitla, Michael Ferguson, John Gamon, Joe Luck, Tami M. Brown-Brandl, Bertrand Clarke, Taro Mieno

Strategic Discussions for Nebraska

Nebraska's Advantage: Productive Agriculture and Bountiful Natural Resources

You can’t talk about healthy production systems and natural resource systems without also thinking about the resilience. We need to harmonize production agriculture in a way that ensures the resilience of our natural ecosystems and rural communities—all while addressing bundles of grand challenges.

Gateways-Nd: Building The Institutional Infrastructure Towards Viable Postsecondary Stem Education Reform, J.M.D. Motschenbacher, M. Vosen Callens, J. Nyachwaya, E. Berg, J. Ladbury, R. Reichenbach, P. Kelter

School of Natural Resources: Documents and Reviews

No abstract provided.

Ncuwm Talk Abstracts 2019

Nebraska Conference for Undergraduate Women in Mathematics

No abstract provided.

Ncuwm Poster Abstracts 2019

Nebraska Conference for Undergraduate Women in Mathematics

No abstract provided.

Past Invasive Species Advisory Committee (Isac) Members, National Invasive Species Committee

National Invasive Species Council

United States Department of the Interior

National Invasive Species Council

Invasive Species Advisory Committee (ISAC)

Past ISAC Members:

Class 9 (2016-2019)

Class 8 (2011-2016/2017)

Class 7 (2011-2012/2014)

Class 6 (2009-2011/2012)

Class 5 (2008/2009-2010)

Class 4 (2006-2008)

Class 3 (2004-2006)

Class 2 (2002-2004)

Class 1 (1999-2001)

Loss Of Potential Aquatic-Terrestrial Subsidies Along The Missouri River Floodplain, Jeff S. Wesner, David L. Swanson, Mark D. Dixon, Daniel A. Soluk, Danielle J. Quist, Lisa A. Yager, Jerry W. Warmbold, Erika Oddy, Tyler C. Seidel

United States National Park Service: Publications

The floodplains of large rivers have been heavily modified due to riparian development and channel modifications, both of which can eliminate shallow off-channel habitats. The importance of these habitats for aquatic organisms like fishes is well studied. However, loss of off-channel habitat also eliminates habitats for the production of emerging aquatic insects, which subsidize riparian consumers in terrestrial food webs. We used field collections of insect emergence, historical mapping, and statistical modeling to estimate the loss of insect emergence due to channel modifications along eight segments of the Missouri River (USA), encompassing 1566 river km, between 1890 and 2012. We …

Age-0 Walleye Sander Vitreus Display Length-Dependent Diet Shift To Piscivory, Christopher S. Uphoff, Casey W. Schoenebeck, Keith D. Koupal, Kevin L. Pope, W. Wyatt Hoback

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The ontogenetic diet shift to piscivory can be energetically beneficial for fish growth and allows larger, more energetically profitable prey to be consumed. A shift to piscivory may be easier for longer individuals within a cohort due to larger gape size, and an early shift is likely advantageous, potentially leading to increased growth rates and survival. Such length-dependent ontogenetic diet shifts may explain the intracohort variability in length that is common for age-0 walleye (Sander vitreus). The objectives of this study were to describe seasonal intracohort variability in length, identify the timing of the shift to piscivory and …

Ecosystem Size Predicts Social-Ecological Dynamics, Mark A. Kaemingk, Christopher J. Chizinski, Craig R. Allen, Kevin L. Pope

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Recreational fisheries are complex adaptive systems that are inherently difficult to manage because of heterogeneous user groups (consumptive vs. nonconsumptive) that use patchily distributed resources on the landscape (lakes, rivers, coastlines). There is a need to identify which system components can effectively predict and be used to manage nonlinear and cross-scale dynamics within these systems. We examine how ecosystem size or water body size can be used to explain complicated and elusive angler-resource dynamics in recreational fisheries. Water body size determined angler behavior among 48 Nebraska, U.S.A. water bodies during an 11- year study. Angler behavior was often unique and …

Dissemination, Access, Preservation: A Case Study Of Publications From The Organic Agriculture Research And Extension Initiative, Leslie M. Delserone

University of Nebraska-Lincoln Libraries: Faculty Publications

The Organic Agriculture Research and Extension Initiative, administered by USDA-NIFA, is the major federal funder of organic agricultural research. Analysis of 733 publications produced during the initiative’s first five years explored the dissemination of this research, and accessibility to and preservation of the publications. Publications associated with conferences (e.g., abstracts) were most numerous (36%). Many publications (69%) were openly accessible online in 2017 but fewer than 10% of these appeared in a stable digital repository. In four of the eight publication categories, access disappeared over time. No program exists to systematically collect and preserve these outputs of organic agricultural research. …