Digital Commons Network^™

Immunological Feedback Loops Generate Parasite Persistence Thresholds That Explain Variation In Infection Duration, Clayton E. Cressler, Daniel C.G. Metz, David A. Chang Van Oordt, Andrea L. Graham

School of Biological Sciences: Faculty Publications

Infection duration affects individual host fitness and between-host transmission. Whether an infection is cleared or becomes chronic depends on the complex interaction between host immune responses and parasite growth. Empirical and theoretical studies have suggested that there are critical thresholds of parasite dose that can determine clearance versus chronicity, driven by the ability of the parasite to manipulate host immunity. However, the mammalian immune response is characterized by strong positive and negative feedback loops that could generate duration thresholds even in the absence of direct immunomodulation. Here, we derive and analyse a simple model for the interaction between T-cell subpopulations …

A New Species Of Acmaeodera Eschscholtz, 1829 (Coleoptera: Buprestidae) From The State Of Michoacán, Mexico, Jason A. Hansen, Richard L. Westcott

Insecta Mundi

Acmaeodera parasuperba Hansen and Westcott, new species (Coleoptera: Buprestidae), from Michoacán, is described, imaged, and its relationship discussed.

Homocopris Burmeister, 1846 (Coleoptera: Scarabaeidae): Lectotype Designation For Copris Torulosus Eschscholtz, 1822, And Correction On The Geographic Distribution Of The Two Chilean Species, José Mondaca, Fernando Z. Vaz-De-Mello

Insecta Mundi

Homocopris Burmeister, 1846 (Coleoptera: Scarabaeidae) is a small genus of South American dung beetles composed of four species distributed in Argentina, Brazil, and Chile. Recently, a neotype was designated for Copris torulosus Eschscholtz, 1822 (currently H. torulosus) based on the assumption that the original type series had been lost. However, this designation is invalid for failing to comply with articles 75.2, 75.3, 75.3.1, and 75.3.4. of the International Code of Zoological Nomenclature. Furthermore, even if it had been nomenclaturally valid, the neotype now loses its type status because we found a syntype in the material of the Johann …

New Species, New Combinations, Synonymies, And Nomenclatural Discussion For Hispaniolan Longhorned Beetles (Coleoptera: Disteniidae, Cerambycidae), Steven W. Lingafelter, Norman E. Woodley

Insecta Mundi

Fifteen new species of longhorned beetles (Coleoptera: Cerambycidae, Disteniidae) from Hispaniola (the Dominican Republic and Haiti) are diagnosed, described, and illustrated: Novantinoe pilithorax Lingafelter, new species (Disteniini); Eburiola ciferrii Lingafelter, new species (Eburiini), Eburiola konstantinovi Lingafelter, new species (Eburiini), Elaphidion jaragua Lingafelter, new species (Elaphidiini), Linsleyonides guerreroi Lingafelter, new species (Elaphidiini), Compsibidion iviei Lingafelter, new species (Tropidini: Subtribe Neoibidionina), Hormathus albisetosus Lingafelter, new species (Tropidini: Subtribe Compsina), Trichrous eryphoides Lingafelter, new species (Dichophyiini), Plectrocerum woodruffi Lingafelter, new species (Dichophyiini), Oreodera nearnsi Lingafelter, new species (Acrocinini/Acanthoderini), Acrepidopterum fuscum Lingafelter, new species (Apomecynini), Acrepidopterum giesberti Lingafelter, new species (Apomecynini), Estola touroulti Lingafelter, …

Additions To The Knowledge Of The Vietnamese Tiger Beetle Species (Coleoptera: Cicindelidae), Jürgen Wiesner, Christian Kerkering, Miroslav Klícha

Insecta Mundi

Therates tuberosus Fleutiaux, 1893 and Calochroa octogramma octogramma (Chaudoir, 1852) (Coleoptera: Cicindelidae) are newly recorded for Vietnam. Ten additional species are reported to have new provincial records in Vietnam.

Range Extension For Pulchriphyllium Anangu Cumming Et Al., 2023 (Phasmatodea: Phylliidae) And First Record From Maharashtra, Gaurang Tawde, Amol Patwardhan, Royce Cumming

Insecta Mundi

Pulchriphyllium anangu Cumming et al., 2023 is here reported for the first time from Maharashtra state, India. Also, this is the first record of a leaf insect (Phasmatodea: Phylliidae) for Phansad Wildlife Sanctuary, District Raigad, Maharashtra. This sighting represents the northernmost distribution in the Western Ghats for Pulchriphyllium anangu.

Assessing Soil Properties & Suitability For Optimized Irrigation Development In Sudan, Northern Africa, Suhib O. Hamid

Department of Agricultural and Biological Systems Engineering: Masters Project Reports

In Chapter 1, soil properties essential for efficient irrigation are thoroughly assessed. Factors such as soil texture, infiltration rates, and nutrient content are analyzed to provide insights into the selection of modern irrigation systems. Utilizing data collected from 3,192 locations through GPS and laboratory analyses, alongside sophisticated ranking systems, the research determines the most suitable irrigation methods for specific soil series. Findings highlight the remarkable efficiency of drip irrigation across various soil types, contrasting with the consistently lower performance of surface irrigation. The chapter emphasizes the significance of considering soil variability, evapotranspiration, and investment factors in selecting irrigation methods for …

Assessing Even Flat-Fan Nozzles For Spot Spray Herbicide Applications, Thiago H. Vitti

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide efficiency in row-crop agriculture can be improved using precision technologies for controlling weeds and minimize agronomic, economic, and environmental impacts. Site-specific weed management technologies, such as spot-sprayers, allow herbicides to be sprayed only where weeds are present in the field. Understanding application parameters and their influence on weed control for site-specific spot-spray applications is essential. This research involved laboratory and greenhouse studies to investigate the coverage and coefficient of variation (CV) of even flat-fan spray nozzles under different spot-spray application scenarios, as well as the effect of weed size and application method on the control of various weed species …

Identifying Genes Linked To Variation In Metabolic And Whole Plant Phenotypes Using Data From Genome Resequencing, Transcriptomics, And Metabolic Profiling Of A Field-Grown Maize Diversity Panel, Ramesh Kanna Mathivanan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize metabolism is highly complex and influenced by genetic variation, yet the specific genes contributing to this variation and their links to non-metabolic traits remain less understood. To address this knowledge gap, we identified genes involved in maize metabolic variation and linked them to non-metabolic traits. We utilized a quadruplicate dataset of whole genome resequencing, transcriptomic, metabolic, and whole plant phenotype data from a single common field experiment of 660 diverse maize inbred lines. Leaf samples were collected shortly before flowering and analyzed using GC-MS for 26 metabolites. A Resampling Model Inclusion Probability Genome-Wide Association Study (RMIPGWAS) of approximately 2.6 …

Ultrashort Pulsed Laser Treatment Is Effective At Sterilizing Metal Surfaces For Planetary Protection, Kaleb Mcquillan

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

To prevent forward contamination from microbes aboard spacecraft intended for exploration of solar system bodies there is a need for effective sterilization methods. However, current techniques are both time-consuming and expensive. For example, dry heat sterilization requires removal from the assembly site and several days of treatment. Furthermore, some components such as optics and electronics are not compatible with current sterilization techniques. In this thesis, a novel femtosecond laser surface processing technique for the rapid sterilization of spacecraft hardware is reported. Femtosecond lasers produce extremely high photon fluxes (10²⁹ photons/s*cm², ~0.03 J/cm²) in extremely short …

Preschool Executive Control And Adolescent Dietary Outcomes: Highlighting The Role Of Context, Marla Lopez

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Research has identified executive control (EC) as an important predictor of dietary outcomes among youth. Executive control in preschool age has been shown to predict important outcomes across development for youth, indicating that good, foundational EC abilities are associated with successful development in later life. Dietary outcomes across adolescence, an important transitional period for establishing healthy eating behaviors, are understudied. Therefore, the current study sought to link two critical developmental periods, examining how preschool EC is associated with dietary trajectories across adolescence. Further, the study aimed to identify how contextual factors, specifically indices of socio-economic status and the home and …

Applications Of Artificial Intelligence On Drought Impact Monitoring And Assessment, Beichen Zhang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Drought, a prevalent and consequential natural disaster, poses widespread, indirect challenges across environmental and societal dimensions. Despite considerable focus on monitoring meteorological and hydrological drought and studying their characteristics, there is a gap in assessing its multifaceted impacts, especially on societal sectors. The dissertation comprises three research essays utilizing artificial intelligence to quantitatively study multi-dimensional drought impacts. The first essay leveraged deep learning and natural language processing to predict multi-dimensional drought impacts from textual datasets, including social media, news media, and citizen scientist reports. The findings demonstrate superior performance over traditional methods and unveil the spatial and temporal heterogeneity of …

Investigating Associations Between Activity And Performance Traits Across Three Breeds Of Swine Using NuTrack, Dalton R. Obermier

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Feed costs are the largest contributor towards the variable costs of pork production. To decrease feed cost while not sacrificing output requires an improvement in feed efficiency (FE). A potential approach to expand available phenotypes associated with FE is with activity tracking, yet the relationship between activity and performance is not well defined in swine. Therefore, the objective of this study was to quantify associations between activity and feed intake traits. Average daily feed intake (ADFI) was collected on 1,766 purebred boars of three genetic lines (Duroc, Landrace, and Yorkshire) using FIRE (Osborne Industries, Inc., Osborne, Kansas) at a testing …

Optimizing Scalability For Formal Analysis With Evolutionary Algorithm, Jianghao Wang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Predominantly employed to tackle hardware validation challenges in the early years, formal methods have since expanded to software engineering, introducing a significant level of rigor and precision to software analysis. Its use of mathematical notations and logical reasoning allows for abstract modeling of programs, enabling researchers and engineers to perform a multitude of analysis tasks to verify system dependability and rigorously prove the correctness of system properties. Despite the availability of many automated analysis tools including those considered lightweight, the practical adoption of formal methods in software development has been limited due to scalability concerns, especially when applied to large …

Real-Time Cerebral Oximetry During Cognition, Sensorimotor Control, And Pneumotactile Perception In Neurotypical Adults, David J. Sanchez

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Continuous-wave functional near-infrared spectroscopy (cw-fNIRS) is a non-invasive brain imaging methodology designed to measure neural activity by detecting hemodynamic responses i.e., adjustments in blood flow and oxygenation to meet the brain’s metabolic needs. Findings on the use of cw-fNIRS by speech-language pathologists (SLP) and neuroscientists to assess cerebral hemodynamic responses during communication assessment and treatment are limited. This study aims to explore the brain’s metabolic response to various sensory processing tasks in neurotypical adults.

This research examines the relationship between cognitive load, sensorimotor control, and somatosensory processing, and hemodynamic modulation in the prefrontal cortex of 18 right-handed neurotypical adults. The …

Virion Composition Of Wheat Streak Mosaic Virus (Wsmv) And Its Use As An Expression Vector, Sourav Pal

Department of Plant Pathology: Dissertations, Theses, and Student Research

Wheat (Triticum aestivum L.) is the principal cereal grown in the United States, whose production ranks third in the world. Bacterial leaf streak (BLS) disease caused by Xanthomonas translucens pv. undulosa (xtu) is an emerging threat to wheat production in the Great Plains region of the United States of America. In this study, we engineered sequences encoding several plant-based antimicrobial peptides (AMP) were engineered into the WSMV genome and examined their effect on BLS disease in wheat infected by WSMV harboring AMP sequences. Out of nine AMPs, wheat expressing Ace-AMP1, PDF13, TK-AMP-D1, and TK-AMP-D3 produced resistance against the …

Microbial Transfer And Cross-Contamination In Milling Facilities And Pathogen Survival In Milled Products And Baking Mixes, Aryany Leticia Peña-Gomez

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Wheat-based products, traditionally considered low-risk due to their low moisture content, have been involved in numerous foodborne outbreaks in recent decades. Salmonella and Shiga toxin-producing Escherichia coli (STEC) present in these products have caused significant numbers of hospitalizations and massive product recalls. The microbiological quality of flour is highly dependent on the incoming wheat, which is vulnerable to contamination from field to milling. Once pathogens are present in flour, they can survive for extended periods. Implementing strategies to mitigate the risk of pathogen contamination throughout the wheat processing chain is critical.

This study evaluated the distribution of E. coli …

Development And Validation Of Aronia Melanocarpa Berry Recipes For Home Canning: Integrating Thermal Lethality Studies, Microbiological Safety, And Antioxidant Analysis, Juan Diego Villegas Posada

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Aronia melanocarpa, also known as chokeberry, is gaining popularity for its high antioxidant content and health benefits, including reducing oxidative stress and inhibiting cancer gene expression. However, its characteristic bitterness and astringency limit its broader appeal. Chokeberry's low pH places it in the acid foods category, making it ideal for safe home canning. An acidic environment combined with heat treatment inhibits harmful bacteria like Clostridium botulinum. Home canning preserves food by sealing it in airtight containers and heating it to destroy spoilage microorganisms.

Developing safe and palatable recipes is essential for making chokeberries suitable for home canning. A …

Transforming Grassland Conservation: Challenges And Opportunities Across Law, Policy, And Human Dimensions, Conor D. Barnes

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Great Plains social-ecological systems are facing growing pressure from complex, ‘wicked’ problems. Addressing these problems will require integrating ecological resilience and complex systems thinking concepts into our legal framework in order to better reflect the changing ecological reality of the Great Plains and promote flexibility and adaptability in the face of that change. In this dissertation, I examine how past and present policy priorities have affected social-ecological systems on the Great Plains, and how ecological resilience and complex systems thinking might be applied to grassland management policy. In Chapter 2, I examine the rapid progress made in the adoption of …

Development Of An Egg-Specific Mass Spectrometry Targeted Method For Processed Food Matrices, Liyun Zhang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Egg is a major food allergen in the United States, causing symptoms from mild reactions to severe anaphylaxis. An egg-free diet remains the only management approach for egg allergy. Therefore, individuals and families living with egg allergies rely heavily on allergen statements on packaged food products for informed food choices. Currently, no labeling regulations exist for unintentionally introduced allergens, posing risks to consumers with egg allergies. Therefore, it is critical to have an accurate and reliable detection method in allergen control and management to determine the concentration of total protein from allergenic food sources. Traditional enzyme-linked immunosorbent assays are routinely …

Development Of Feature Extraction Models To Improve Image Analysis Applications In Cancer, Yu Shi

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cancer poses a significant global health challenge. With an estimated 20 million new cases diagnosed worldwide in 2022 and 9.7 million fatalities attributable to the disease, the economic burden of cancer is immense. It impacts healthcare systems and imposes substantial costs for its care on patients and their families. Despite advancements in early detection, prevention, and treatment that have reduced overall cancer mortality rates, the growing prevalence of cancer, particularly among younger individuals, remains a pressing issue.

Recent advancements in medical imaging technology have progressed significantly with the help of emerging computer vision and artificial intelligence (AI) technology. Despite these …

Semigroup Well-Posedness And Finite Element Analysis Of A Biot-Stokes Interactive System, Sara Mcknight

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The coupling of a porous medium modeled by the Biot equations and a fluid has many biological applications. There are numerous ways by which to model the fluid and to couple the porous medium with the fluid. This particular model couples the Biot equations to Stokes flow along the boundary, through the Beavers-Joseph-Saffman conditions. We address semigroup well-posedness of the system via an inf-sup approach, which along the way requires consideration of a related but uncoupled static Biot system. We also present the results of finite element analysis on both the uncoupled Biot system and the coupled system.

Advisor: Sara …

Integrating Water And Nitrogen Management For Sustainable Agriculture: Optimizing Resource Use Efficiency And Maximizing Crop Productivity, Jiaming Duan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Advisors: Derek Heeren and Daran Rudnick Maize, accounting for over 95% of national grain production in the United States, is highly sensitive to water and nitrogen (N) inputs. Conventional agricultural practices often lead to excessive application, causing groundwater contamination through nitrate leaching. Therefore, there is a demand for integrating water and nitrogen management with innovative scheduling methods for sustainable agricultural development.

This dissertation first reviewed two decades of U.S.-based research, highlighting the optimal management of water and N to enhance yield, water use efficiency (WUE), and nitrogen use efficiency (NUE). Findings indicate that maintaining optimal levels of N and water …

"I Will Write Mad Stories": The Hysterical "I" In The Diaries Of George Eliot, Charlotte Forten Grimke, Virginia Woolf, And Sylvia Plath, Jaclyn Marie Swiderski

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation focuses on the long history of hysteria and the ways in which it has been used to denigrate and silence disabled women. Women diagnosed as hysterical, by either the medical establishment or the court of public opinion, are denied the right to generate knowledge about and for themselves – they are epistemologically disabled. The author argues that hysterical women have unique ways of looking at and understanding the world which push back against their epistemological disablement. In order to uncover some of this history of hysterical women, this dissertation uses the diaries of four “hysterical” women over the …

A Data-Driven Discovery System For Studying Extracellular Microrna Sorting And Rna-Protein Interactions, Sasan Azizian

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Interactions between microRNAs (miRNAs) and RNA-binding proteins (RBPs) are pivotal in miRNA-mediated sorting, yet the molecular mechanisms underlying these interactions remain largely understudied. Few miRNA-binding proteins have been verified, typically requiring extensive laboratory work. This study introduces DeepMiRBP, a novel hybrid deep learning model designed to predict microRNA-binding proteins. The model integrates Bidirectional Long Short-Term Memory (Bi-LSTM) networks with attention mechanisms, transfer learning, and cosine similarity to offer a robust computational approach for inferring miRNA-protein interactions.

DeepMiRBP is implemented through two distinct architectures. The first architecture employs a Y-shaped model that uses Bi-LSTM networks and transfer learning to extract contextual …

Role Of The Malate Dehydrogenase-Citrate Synthase Metabolon In Metabolic Flux Regulation, Joy John Omini

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The TCA cycle enzymes- malate dehydrogenase (MDH) and citrate synthase (CS)- interact to transfer oxaloacetate from the active site of MDH to the active site of CS, through an electropositive channel. The MDH-CS metabolon is highly conserved across different domains of life, and it has been associated with many advantages. Some of these advantages include protecting oxaloacetate from competing enzymes like aspartate aminotransferase, allowing the thermodynamically unfavorable MDH forward reaction to occur and increasing the local concentration of oxaloacetate within the active site of CS. These advantages and conservativeness of the MDH-CS metabolon indicate that it is highly important to …

Dynamics Of Soybean Seed Protein And Oil Content With Depth In The Canopy, Hafith Furqoni

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding the physiological basis of variation in seed composition is critical for optimizing soybean seed composition. Also, studying dry matter accumulation and nitrogen uptake, partitioning, and redistribution in soybeans is crucial due to its direct impact on seed yield levels and overall plant productivity. This study aimed to test the primary proxy influencing seed protein and oil content, which is their accumulation rate determined by the assimilate supply per seed. The second objective was to comprehensively understand soybean dry matter (DM) accumulation and nitrogen (N) uptake, partitioning, and removal patterns within the canopy. Field experiments were conducted using two soybean …

Occurrence, Inputs, And Ecological Significance Of Antibiotics And Pharmaceuticals In Western Nebraska Streams, Katelyn F. Glause

School of Natural Resources: Dissertations, Theses, and Student Research

Few emerging environmental contaminants are as concerning as antibiotics. Human and animal health benefits greatly from the prudent use of antibiotics, yet we give little thought to environmental release of these biologically active compounds. Environmental occurrence of these and other pharmaceutical compounds must be measured in different environmental compartments such as municipal wastewater and in the vicinity of large animal feeding operations to understand potential effects. This study reports the results of a monitoring study in western Nebraska, with a large population of livestock and smaller but more concentrated population of humans, comparing the relative environmental concentrations and loading from …

Remotely Sensed Early Warning Of Algal Blooms In An Eastern Nebraska Reservoir: A Comparison Of Temporal And Spatial Indicators, Mercy Kipenda

School of Natural Resources: Dissertations, Theses, and Student Research

Cyanobacterial harmful algal blooms (CyanoHABs) detrimentally affect human, animal, and ecosystem health. Remotely sensed early warning systems for cyanoHABs in inland lakes could contribute to more proactive water quality monitoring and help mitigate negative impacts. Advances in freely available remote sensing imagery, with finer spatial, temporal, and spectral resolutions, present new opportunities for the development and comparative analysis of methods to detect sudden deterioration in lake water quality. In this thesis, I compared and tested for temporal and spatial early warning signals of cyanoHABs in field-based and remotely sensed datasets from 2019 to 2023 in Pawnee Lake in southeast Nebraska, …

Impacts Of Invasive Carp And Their Population Dynamics On Fish Communities In The Missouri River, Joshua F. Kocik

School of Natural Resources: Dissertations, Theses, and Student Research

Invasive Carp species: Silver Carp Hypophthalmichthys molitrix, Bighead Carp Hypophthalmichthys nobilis, Grass Carp Ctenopharyngodon idella, and Black Carp Mylopharyngodon piceus have both potential and realized abilities to negatively impact native species. Specifically, Bighead Carp and Silver Carp as filter feeders have been theorized to be detrimental not only to adult filter-feeding species, but to many fish which rely on plankton and algae in their early-life stages. The impact of Invasive Carp on the overall fish community remains underexplored. The fish communities below Gavins Point Dam (Invasive Carp present) and Fort Randall Dam (Invasive Carp absent) afford the …