Digital Commons Network^™

Spatiotemporal Plasticity In Reproductive Readiness And Recruitment Of Ichthyoplankton Of Invasive Silver Carp Along A Western Invasion Front, Jessi L. Urichich

School of Natural Resources: Dissertations, Theses, and Student Research

Individuals of invasive species at the edge of their invasion distribution may exhibit enhanced reproductive capacity and phenotypic plasticity leading to accelerated range expansion. Environmental conditions in highly fluctuating environments at distribution edges may either promote or hinder such reproductive readiness and phenotypic plasticity. Dynamic habitat conditions and periodic disturbances experienced in prairie streams, such as drought (e.g., low-flow, high water temperatures), may potentially reduce suitable spawning environments and ichthyoplankton recruitment of invasive Silver Carp, potentially slowing range expansion or enabling management strategies that reduce abundance in the absence of a reproducing population. Female Silver Carp batch fecundity, Gonadosomatic Index …

Assessing, Restoring, And Centering Social–Ecological Relationships For Advancing Social–Ecological Resilience In The Northern Great Plains Grasslands, Katia Pilar Carranza Bernal

School of Natural Resources: Dissertations, Theses, and Student Research

The Northern Great Plains grasslands are social-ecological systems that were shaped by evolutionary and Indigenous social-ecological relationships. European colonization disrupted many of these interactions, including the coupling of fire and grazing, and degraded social-ecological resilience, shifting these grasslands to a new state. For those reasons, my research focused on assessing, restoring, and centering evolutionary and Indigenous social-ecological relationships for advancing social-ecological resilience in the Northern Great Plains grasslands. I first performed a study in the Nebraska Sandhillls assessing the potential of patch-burn grazing to support grassland resilience by comparing its effects to those of rotational grazing. Through vegetation and bird …

We're Swarming Again! Swarming, Collectivity, And Trope: The Case Of Extinction Rebellion, Tyler J. Behymer

Department of Communication Studies: Dissertations, Theses, and Student Research

This thesis explores the rhetoric of the eco-movement Extinction Rebellion, focusing on the use of swarming and nature tropes to mobilize collective action and revivify contemporary notions of collectivity. Drawing on rhetoric of social movement scholarship, cultural studies, and psychoanalysis, this essay theorizes swarming as a tropological economy that expands the conditions of propriety in the context of collectivity. Through an analysis of Extinction Rebellion’s discourse, this study demonstrates how the naturalization of swarming tropes works in various ways to rewild conventional political discourse, galvanize disruptive collective assembly, and challenge green neoliberalism.

Advisor: Casey Ryan Kelly

Integration Of Matlab And Machine Learning To Accelerate Evaluation Of Biological Activity In Agricultural Soils And Promote Soil Health Improvement Goals, Andrew Stiven Ortiz Balsero

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Traditionally, assessments of soil biological activity have been confined to laboratory settings, creating a disconnect with practical in-field methods. To bridge this gap, cotton fabric degradation has been used to illustrate soil microbial activity under different management practices. While effective, these demonstrations are subjective and labor-intensive.

Researchers have explored using image processing software like ImageJ and Adobe Photoshop to streamline this process. Although these tools accurately quantified fabric degradation under varying soil conditions, the methods remained labor-intensive and complex. Consequently, these methods were still not ideal for on-farm use by agricultural practitioners.

To further address labor and complexity limitations, the …

Candida Albicans Farnesol Synthesis And Secretion, Daniel J. Gutzmann

School of Biological Sciences: Dissertations, Theses, and Student Research

Candida albicans is a polymorphic fungus and opportunistic commensal found primarily in the gastrointestinal and skin of healthy individuals. Several barriers prevent C. albicans from causing disease including a healthy immune system and microbiome. When these barriers become comprised, C. albicans can transition to a pathogen and disseminate through the intestinal mucosa leading to life-threatening bloodstream and invasive infections with mortality rates of up to 64%. Morphogenic plasticity is key to this transition and impacts virulence, adaptation to different host environments, and evasion of host immune responses. One regulator of morphogenesis is farnesol. Farnesol is a secondary metabolite of …

Developing A Comprehensive Genome-Scale Metabolic Model For The Arabidopsis Thaliana Root System, Lohani Esterhuizen

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Arabidopsis thaliana (Arabidopsis) is the most well-established model plant to date. Being the first plant to have its genome mapped, studies on Arabidopsis have provided insurmountable insights into the physiological and biochemical nature of plants. Methods that allow us to computationally study the metabolism of organisms include the use of genome-scale metabolic models (GEMs). Despite its popularity, no GEM currently maps the metabolic activity in the root system of Arabidopsis, which is the first organ to face and respond to stress conditions in the soil. This work aims to develop and implement a comprehensive GEM of the Arabidopsis root system …

On Neumann Boundary Conditions For Nonlocal Models With Finite Horizon, Scott Alex Hootman-Ng

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Nonlocal models are have recently seen an explosive interest and development in the context of fracture mechanics, diffusion, image processing, population dynamics due to their ability to approximate differential-like operators with integral operators for inherently discontinuous solutions. Much of the work in the field focuses on how concepts from partial differential equations (PDEs) can be extended to the nonlocal domain. Boundary conditions for PDEs are crucial components for applications to physical problems, prescribing data on the domain boundary to capture the behavior of physical phenomena accurately with the underlying model. In this thesis we specifically examine a Neumann-type boundary condition …

Understanding The Role Of The Microbiome In Infectious Disease, Developing Surveillance Tools, And Engineering Direct Fed Microbials To Improve Animal Health, Alison Clare (Bartenslager) Neujahr

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

With the ever-increasing demand for animal protein and the continual need for the assurance of proper animal welfare, investigations of preventative strategies for diseases is greatly needed. Specifically in the cattle industry, infectious bovine keratoconjunctivitis (IBK), commonly known as pinkeye, causes a large economic loss to the producer. However, effective mitigation strategies are lacking. Partly, this is due to the lack of studies investigating the ocular microbiome. Therefore, studies investigating the bovine ocular microbiome are greatly needed to better understand IBK disease etiology. To this end, we investigated the changes in the ocular microbiome in animals before, during and after …

Candida Albicans Farnesol Synthesis And Secretion, Daniel John Gutzmann

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Candida albicans is a polymorphic fungus and opportunistic commensal found primarily in the gastrointestinal and skin of healthy individuals. Several barriers prevent C. albicans from causing disease including a healthy immune system and microbiome. When these barriers become comprised, C. albicans can transition to a pathogen and disseminate through the intestinal mucosa leading to life-threatening bloodstream and invasive infections with mortality rates of up to 64%. Morphogenic plasticity is key to this transition and impacts virulence, adaptation to different host environments, and evasion of host immune responses. One regulator of morphogenesis is farnesol. Farnesol is a secondary metabolite …

Elementary Science Teacher Self-Efficacy In The Context Of Ngss-Aligned Curriculum Enactment: An Explanatory Sequential Mixed Methods Study, Wesley P. Sliger

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

A confluence of circumstances in educational policy and teacher preparation have placed a low priority on elementary science teacher preparation. Historically, a lack of elementary science teachers’ subject matter knowledge and pedagogical knowledge has negatively impacted science teaching self-efficacy, which is linked to many teacher and student outcomes. Meanwhile, the past decade has ushered in the Next Generation Science Standards (NGSS) and the modern era of reform-based science teaching. The emergence of self-proclaimed NGSS-aligned resources has outpaced academic examination regarding professional development (PD), curriculum implementation, and curriculum enactment. The purpose of this study was to use social cognitive theory to …

Phylogenetic And Biogeographic Patterns Of Devonian Proetid Trilobites, Katherine Jane Jordan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Ecological disruption events such as mass extinctions can result in the permanent alteration in clades and ecosystems. A pivotal time of change during the history of life on Earth can be seen in the Devonian Period. During this time, major transitions in marine and terrestrial system resulted in a different world at the end of the period in comparison to the beginning. One such group most heavily impacted were trilobites. Trilobites were reduced to one order by the end of the Devonian: Proetida. My dissertation focuses on phylogenetic, biogeographic, and paleocommunity patterns of this group, known as the last of …

Nanoparticle-Based Approaches To Brain Injury And Studies For Improved Detectability Toward Better Delivery Characterization, Hunter A. Miller

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The clinical translation of nanoparticle (NP) therapeutics for brain injury has been slow despite preclinical promise. The causes of disconnect between preclinical and clinical outcomes is multifactorial, but insufficient delivery is noteworthy. Preclinical delivery characterization often lacks, obscuring mechanisms of failure, slowing development. Multimodal imaging is a promising strategy to improve delivery characterization, combining the strengths of various modalities. Magnetic resonance imaging (MRI) and luminescence imaging are a suitable combination for multimodal imaging, expanding the spatial and temporal resolution of either alone, but further material development is needed to enhance detectability. One problem is the interference of cellular autofluorescence in …

Integrated Multi-Omics Analysis Of Cerebrospinal Fluid In Postoperative Delirium, Bridget A. Tripp, Simon T. Dillon, Min Yuan, John M. Asara, Sarinnapha M. Vasunilashorn, Tamara G. Fong, Sharon K. Inouye, Long H. Ngo, Edward R. Marcantonio, Zhongcong Xie, Towia A. Libermann, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

Preoperative risk biomarkers for delirium may aid in identifying high-risk patients and developing intervention therapies, which would minimize the health and economic burden of postoperative delirium. Previous studies have typically used single omics approaches to identify such biomarkers. Preoperative cerebrospinal fluid (CSF) from the Healthier Postoperative Recovery study of adults ≥ 63 years old undergoing elective major orthopedic surgery was used in a matched pair delirium case–no delirium control design. We performed metabolomics and lipidomics, which were combined with our previously reported proteomics results on the same samples. Differential expression, clustering, classification, and systems biology analyses were applied to individual …

Aposematic-Habitat Correlation In Nearctic Species Of Episyron Schiødte (Hymenoptera: Pompilidae: Pompilinae: Pompilini), Frank E. Kurczewski

Insecta Mundi

Three Nearctic species of Episyron Schiødte (Hymenoptera: Pompilidae: Pompilinae: Pompilini) were examined morphologically, geographically, and ecologically with intent to infer an aposematic correlation with habitat type, protarsal digging rake morphology, and host spider association. Episyron quinquenotatus quinquenotatus (Say) and E. conterminus cressoni (Dewitz), two subspecies with extensive aposematic markings and more and longer protarsal comb spines, were associated with bare or sparsely vegetated sandy soils near water courses. Episyron biguttatus biguttatus (Fabricius), with few aposematic markings and less and shorter protarsal comb spines, was associated with more densely vegetated terrain and gravelly and loamy soils. Three subspecies of Anoplius …

An Analysis Of The Genus Aleyrodes Latreille (Hemiptera: Sternorrhyncha: Aleyrodidae) In California, With Three Synonymies, Natalia Von Ellenrieder, Raymond J. Gill

Insecta Mundi

Abstract

Based on the examination of an extensive series of specimens and available type material, three out of the six species of Aleyrodes Latreille (Hemiptera: Sternorrhyncha: Aleyrodidae) described from California, A. diasemus Bemis, 1904, A. essigi Penny, 1922, and A. osmaroniae Sampson, 1945, are newly synonymized under A. spiraeoides Quaintance, 1900. The species of Aleyrodes reported from California are keyed and diagnosed based on characters from puparia and male genitalia, and character variability is documented and illustrated.

Resumen

Basado en el examen de una extensa serie de especímenes y material tipo disponible, tres de las seis …

Revision Of Trachyderines Related To Sphaenothecus From North America With Description Of Three New Genera, And New Species Of Lophalia Casey, 1912, Mannophorus Leconte, 1854, And Ischnocnemis Thomson, 1864 (Coleoptera: Cerambycidae: Cerambycinae: Trachyderini), Bryan K. Eya

Insecta Mundi

The genera Lophalia Casey, 1912, Mannophorus LeConte, 1854 and Ischnocnemis Thomas, 1864 (Coleoptera: Cerambycidae: Cerambycinae: Trachyderini) are recharacterized by providing additional morphological features to enhance the delineation of these genera. Two species, Lophalia cribricollis Bates, 1892 and Ischnocnemis luteicollis (Bates, 1885), are transferred to a new genus Lophaliamorpha Eya with Lophaliamorpha luteicollis (Bates) new combination as the type species. Ischnocnemis skillmani Chemsak and Hovore, 2010 is transferred to a new genus Paramannophorus Eya with Paramannophorus skillmani (Chemsak and Hovore) new combination as the type species. A new genus Microteroschema is created to accommodate two additional species from Mexico: Microteroschema parvum …

A New Species Of Gaindaphodius Král And Mencl From Nepal (Coleoptera: Scarabaeidae: Aphodiinae), Stefano Ziani

Insecta Mundi

A new species from Nepal, Gaindaphodius marigold (Coleoptera: Scarabaeidae: Aphodiinae), is described, illustrated and compared with G. gainda Král and Mencl, 2024, the only other species in the genus Gaindaphodius Král and Mencl, 2024.

Three Essays In Food Security And The U.S. Sugar Program, Jacob Michels

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Chapter one devises an approach to adjust estimates of the number of food insecure for changes in sedentarism over time. Existing methodologies are biased upwards and date back several decades. We build a household model utilizing a Stone-Geary utility function, which rationalizes households shifting their labor decisions towards sedentary activities. Our comparative statics examine the impacts of changes in the productivities of sedentary types, as opposed to my physically demanding types, of activities and also their returns. Our empirical approach is informed by our theoretical model and comparative statics, in which we construct a unique pseudo-panel dataset. Sitting time serves …

Effect Of Beef Heifer Development Systems Utilizing Corn Residue And Late Summer Planted Cover Crops On Growth, Reproductive Performance, And Economics, Hannah F. Speer, Robert A. Cushman, Harvey Freetly, Jay Parsons, Jessica Windh, M. E. Drewnoski

Department of Agricultural Economics: Faculty Publications

The objective of this study was to evaluate growth and reproductive performance of heifers developed using 3 different winter systems in the midwestern U.S. Spring-born heifers (n = 1,156; 214 d of age; SD ± 17 d) were used in a 3-yr study to evaluate performance in winter development systems, which utilized cover crop (CC) and corn residue grazing. Heifers were assigned to 1 of 3 treatments: grazing corn residue with 0.77 kg/d dried distillers grains (CD) or 1.69 kg/d wheat midds (CW) supplementation followed by a grower ration in the drylot, or grazing late summer planted oat-brassica CC followed …

Exploring Chilling Stress And Recovery Dynamics In C4 Perennial Grass Of Miscanthus Sinensis, Karolina Sobańska, Monika Mokrzycka, Martyna Przewoźnik, Tomasz Pniewski, Katarzyna Glowacka

Department of Biochemistry: Faculty Publications

The increasing cultivation of perennial C4 grass known as Miscanthus spp. for biomass production holds promise as a sustainable source of renewable energy. Unlike the sterile triploid hybrid of M. × giganteus, which cannot reproduce through seeds, M. sinensis possesses attributes that could potentially address these limitations by effectively establishing itself through seed propagation. This study aimed to investigate how 18 genotypes of M. sinensis respond to chilling stress and subsequent recovery. Various traits were measured, including growth and biomass yield, the rate of leaf elongation, and a variety of chlorophyll fluorescence parameters, as well as chlorophyll …

Records Of The Red-Bellied Snake (Storeria Occipitomaculata) In Central And Northeastern Nebraska, Taylor Lowe, Sarah M. Bailey, Dan D. Fogell, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Red-bellied Snakes (Storeria occipitomaculata) are a distinctive, small species that live in moist woodlands throughout much of eastern North America. In the Great Plains, isolated populations exist in South Dakota, Wyoming, and Nebraska. In Nebraska, the only known extant population occurs in wooded riparian habitats along the Big Bend Reach of the Platte River in southcentral parts of the state. Herein, we report on new county records of Red-bellied Snakes in Nebraska, with two observations from Merrick County along the Platte River adjacent to known populations and two observations of a new population in the extreme northeastern part …

Greater Aperture Counteracts Effects Of Reduced Stomatal Density On Water Use Efficiency: A Case Study On Sugarcane And Meta-Analysis, Daniel Lunn, Baskaran Kannan, Amandine Germon, Alistair Leverett, Thomas E. Clemente, Fredy Altpeter, Andrew D.B. Leakey

Department of Agronomy and Horticulture: Faculty Publications

Stomata regulate CO₂ and water vapor exchange between leaves and the atmosphere. Stomata are a target for engineering to improve crop intrinsic water use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) and reduce stomatal conductance (g_sw). However, the quantitative relationship between reduced SD, g_sw, and the mechanisms underlying it is poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as a case study alongside a meta-analysis of data from 10 species. Transgenic expression of EPIDERMAL PATTERNING FACTOR 2 from Sorghum bicolor (SbEPF2) in sugarcane …

Development And Evaluation Of An Expedited System For Creation Of Single Walled Carbon Nanotube Platforms, Ivon Acosta Ramirez, Omer Sadak, Wali Sohail, Xi Huang, Yongfeng Lu, Nicole M. Iverson

Department of Electrical and Computer Engineering: Faculty Publications

Single-walled carbon nanotubes (SWNT) have a strong and stable near-infrared (nIR) fluorescence that can be used to selectively detect target analytes, even at the single molecule level, through changes in either their fluorescence intensity or emission peak wavelength. SWNTs have been employed as NIR optical sensors for detecting a variety of analytes. However, high costs, long fabrication times, and poor distributions limit the current methods for immobilizing SWNT sensors on solid substrates. Recently, our group reported a protocol for SWNT immobilization with high fluorescence yield, longevity, fluorescence distribution, and sensor response, unfortunately this process takes 5 days to complete. Herein …

Optimized Gadolinium-Do3a Loading In Raftpolymerized Copolymers For Superior Mr Imaging Of Aging Blood–Brain Barrier, Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest Kievit, Anthony J. Covertine

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. …

Revisiting Fr13a For Submergence Tolerance: Beyond The Sub1a Gene, Waseem Hussain, Mahender Anumalla, Abdelbagi M. Ismail, Harkamal Walia, Vistas Kumar Singh, Ajay Kohli, Sankalp Bhosale, Hans Bhardwaj

Department of Agronomy and Horticulture: Faculty Publications

Rice landrace FR13A is the original donor of the SUB1A gene. FR13A harbors hidden genetic variation that surpasses the submergence tolerance level of SUB1A. Here, we provide an overview of the unique features of FR13A associated with exceptional recovery ability that have been overlooked since the significant discovery of the SUB1 locus. We present a detailed overview of how to underpin the new genes associated with the recovery ability seen in FR13A. Finally, we propose a unique approach to develop rice cultivars that surpass submergence tolerance beyond the SUB1 locus from FR13A.

Lymphoid Tissues Contribute To Plasma Viral Clonotypes Early After Antiretroviral Therapy Interruption In Siv-Infected Rhesus Macaques, Antonio Solis-Leal, Nongthombam Boby, Suvadip Mallick, Yilun Cheng, Fei Wu, Grey De La Torre, Jason Dufour, Xavier Alvarez, Vinay Shivanna, Yaozhong Liu, Christine M. Fennessey, Jeffrey D. Lifson, Qingsheng Li, Brandon F. Keele, Binhua Ling

School of Biological Sciences: Faculty Publications

The rebound-competent viral reservoir, composed of a virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation of systemic viral replication and rebound viremia after ART interruption (ATI), remains the biggest obstacle to treating HIV infection. A better understanding of the cellular and tissue origins and the dynamics of viral populations that initiate rebound upon ATI could help develop therapeutic strategies for reducing the rebound-competent viral reservoir. In this study, barcoded simian immunodeficiency virus (SIV), SIVmac239M, was used to infect rhesus macaques to enable monitoring of viral barcode clonotypes contributing to virus detectable in plasma after ATI. …

Using Machine Learning To Determine A Functional Classifier Of Reward Responsiveness And Its Association With Adolescent Psychiatric Symptomatology, Robert James Richard Blair, Johannah Bashford-Largo, Ahria Dominguez, Matthew Dobbertin, Karina S. Blair, Sahil Bajaj

Center for Brain, Biology, and Behavior: Faculty Publications

Background. Machine learning (ML) has developed classifiers differentiating patient groups despite concerns regarding diagnostic reliability. An alternative strategy, used here, is to develop a functional classifier (hyperplane) (e.g. distinguishing the neural responses to received reward v. received punishment in typically developing (TD) adolescents) and then determine the functional integrity of the response (reward response distance from the hyperplane) in adolescents with externalizing and internalizing conditions and its associations with symptom clusters.

Methods. Two hundred and ninety nine adolescents (mean age = 15.07 ± 2.30 years, 117 females) were divided into three groups: a training sample of TD adolescents …

Second Language Learning In Older Adults Modulates Stroop Task Performance And Brain Activation, Douglas H. Schultz, Alison Gansemer, Kiley Allgood, Mariah Gentz, Lauren Secilmis, Zoha Deldar, Cary R. Savage, Ladan Ghazi Saidi

Center for Brain, Biology, and Behavior: Faculty Publications

Introduction: Numerous studies have highlighted cognitive benefits in lifelong bilinguals during aging, manifesting as superior performance on cognitive tasks compared to monolingual counterparts. Yet, the cognitive impacts of acquiring a new language in older adulthood remain unexplored. In this study, we assessed both behavioral and fMRI responses during a Stroop task in older adults, pre- and post language-learning intervention.

Methods: A group of 41 participants (age: 60–80) from a predominantly monolingual environment underwent a four-month online language course, selecting a new language of their preference. This intervention mandated engagement for 90 minutes a day, five days a week. …

Female Foragers Sometimes Hunt, Yet Gendered Divisions Of Labor Are Real: A Comment On Anderson Et Al. (2023), The Myth Of Man The Hunter, Vivek V. Venkataraman, Jordie Hoffman, Kyle Farquharson, Helen Elizabeth Davis, Edward H. Hagen, Raymond B. Hames, Barry S. Hewlett, Luke Glowacki, Haneul Jang, Robert Kelly, Karen Kramer, Sheina Lew-Levy, Katie Starkweather, Kristen Syme, Duncan N. E. Stibbard-Hawkes

Department of Anthropology: Faculty Publications

Gendered divisions of labor are a feature of every known contemporary hunter-gatherer (forager) society. While gender roles are certainly flexible, and prominent and well-studied cases of female hunting do exist, it is more often men who hunt. A new study (Anderson et al., 2023) surveyed ethnographically known foragers and found that women hunt in 79% of foraging societies, with big-game hunting occurring in 33%. Based on this single type of labor, which is one among dozens performed in foraging societies, the authors question the existence of gendered division of labor altogether. As a diverse group of hunter-gatherer experts, we find …

Evaluating A Precision Sprayer For Detecting Weeds And Spraying Herbicides In Real Time (Spot Spray) For Weed Management In Corn And Soybean, Adam Eric Leise

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide sprayers are becoming larger and more efficient in applications in crop fields. A precise and efficient herbicide application is advantageous in effective weed control. New precision sprayers were introduced in hopes of reducing the amount of herbicide applied. Greeneye Technology and John Deere have developed precision sprayers for the detection of weeds in crop fields, which come equipped with a dual tank system that allows for two different herbicides to be separated from tank to tip. The objectives of this study were to evaluate the broadleaf and grass weed control efficacy of spot spray (SS) technology when applied in …