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Nebraska Summary 1196: John Deere 6175m Ft4, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Does Context Matter? A Multilevel Analysis Of Neighborhood Disadvantage And Children's Sleep Health, Carlyn Graham, Eric N. Reither, Gabriele Ciciurkaite, Dipti Dev, Jamison Fargo

Department of Child, Youth, and Family Studies: Faculty Publications

Objectives: To determine how demographic, socioeconomic, and neighborhood characteristics are associated with bedtimes among US kindergarteners.

Design: Parents reported bedtimes of their children as well as personal, household, and residential characteristics via interviews in the Early Childhood Longitudinal Study-Kindergarten (ECLS-K) Class of 1998-1999. The ECLS-K links individual households to US Census tracts.

Setting: A random selection of 1,280 schools and surrounding communities in the US.

Participants: A random selection of 16,936 kindergarteners and their parents.

Measurements: The 2 outcomes were regular and latest weekday bedtimes of kindergarteners. Through a series of nested multilevel regression models, these outcomes were regressed on …

The Meanings And Ways Of Parental Involvement Among Low-Income Filipinos, Aileen Garcia, Maria Rosario De Guzman

Department of Child, Youth, and Family Studies: Faculty Publications

Parental involvement in children’s education is an integral component of young children’s academic achievement. Although there is clear evidence regarding the benefits of parental involvement, little is known regarding its impacts, manifestations, and conceptualizations in non-Western societies. Given that parenting and child rearing are imbued with cultural meaning in many profound ways (Super & Harkness,1986), this study employed a phenomenological approach and used pakikipagkwentuhan, a data collection procedure drawn from indigenous Filipino Psychology to closely examine how low-income Filipino parents conceptualize parental involvement and its role in their children’s education. Thirty-one parents/caregivers were engaged in conversation and qualitative data …

Promoting Higher Quality Teacher–Child Relationships: The Insights Intervention In Rural Schools, Kathleen Moritz Rudasill, Ray Reichenberg, Jungwon Eum, Jentry Stoneman Barrett, Yuenjung Joo, Emily Wilson, Martinique Sealy

Department of Child, Youth, and Family Studies: Faculty Publications

Children’s relationships with teachers in kindergarten are crucial for academic and social success. Research shows that teacher–child relationships are predicated, in part, on children’s temperament. The “INSIGHTS into Children’s Temperament” intervention was intended to improve children’s and teachers’ understanding of their and others’ temperament, and has been shown to improve children’s social skills and self-regulation in urban, under-resourced schools. The current study is part of a replication of the effects of INSIGHTS with a sample in rural schools. The purpose was to test the effectiveness of INSIGHTS for promoting positive relationships between teachers and children in kindergarten. Two cohorts of …

Does Context Matter? A Multilevel Analysis Of Neighborhood Disadvantage And Children's Sleep Health, Carlyn Graham, Eric Reither, Gabriele Ciciurkaite, Dipti Dev, Jamison Fargo

Department of Child, Youth, and Family Studies: Faculty Publications

Objectives: To determine how demographic, socioeconomic, and neighborhood characteristics are associated with bedtimes among US kindergarteners.

Design: Parents reported bedtimes of their children as well as personal, household, and residential characteristics via interviews in the Early Childhood Longitudinal Study-Kindergarten (ECLS-K) Class of 1998–1999. The ECLS-K links individual households to US Census tracts.

Setting: A random selection of 1,280 schools and surrounding communities in the US.

Participants: A random selection of 16,936 kindergarteners and their parents.

Measurements: The 2 outcomes were regular and latest weekday bedtimes of kindergarteners. Through a series of nested multilevel regression models, these outcomes were regressed on …

Mod$$At: A Hydro-Economic Modeling Framework For Aquifer Management In Irrigated Agricultural Regions, Mani Rouhi Rad, Erin M.K. Haacker, Vaishali Sharda, Soheil Nozari, Zaichen Xiang, A. Araya, Venkatesh Uddameri, Jordan Suter, Prasanna Gowda

Department of Earth and Atmospheric Sciences: Faculty Publications

In this paper, we introduce a hydro-economic modeling framework for the management of groundwater re- sources that are used for irrigated agricultural production. The model, MOD$$AT, can be used to study the costs of aquifer depletion and the net benefits of specific aquifer management policies. MOD$$AT is composed of three components, namely, an economic component, a hydrologic component and an agronomic component. A main goal of this paper is to introduce the hydro-economic model and describe how it can be transferable to different contexts. With this objective in mind, we describe model components step-by-step so that the process of integration …

Hyperparasitic Spore-Forming Eukaryotes (Microsporidia, Haplosporidia, And Myxozoa) Parasitizing Trematodes (Platyhelminthes), Yuliya Ya. Sokolova, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Library Materials

ABSTRACT

Trematodes serve as outstanding hosts for a variety of parasites. This paper restricts those parasites to three groups of non-related taxa parasitizing trematodes and forming invasive spores similar in external morphology, which is why in the past these three groups of parasitic eukaryotes were united into the taxon “Sporozoa” (Kudo, 1924). (1) Trematode-infecting microsporidia (type Microsporidia Balbiani, 1882: Rozellomycota: Holomycota) are represented by more than 30 species. Among those, the species with sequenced barcode (SSU rDNA) are phylogenetically associated with either microsporidia from invertebrates, as the species of the genus Unikaryon (7 species), or fish microsporidia, as the species …

Efficient Coi Barcoding Using High Throughput Single-End 400 Bp Sequencing, Chentao Yang, Yuxuan Zheng, Shangjin Tan, Guanliang Meng, Wei Rao, Caiqing Yang, David G. Bourne, Paul A. O'Brien, Junqiang Xu, Sha Liao, Ao Chen, Xiaowei Chen, Xinrui Jia, Ai-Bing Zhang, Shanlin Liu

Harold W. Manter Laboratory of Parasitology: Library Materials

Background

Over the last decade, the rapid development of high-throughput sequencing platforms has accelerated species description and assisted morphological classification through DNA barcoding. However, the current highthroughput DNA barcoding methods cannot obtain full-length barcode sequences due to read length limitations (for example, a maximum read length of 300 bp for the Illumina’s MiSeq system), or are hindered by a relatively high cost or low sequencing output (e.g. a maximum number of eight million reads per cell for the PacBio’s SEQUEL II system).

Results

Pooled cytochrome c oxidase subunit I (COI) barcodes from individual specimens were sequenced on the …

Repositories For Taxonomic Data: Where We Are And What Is Missing, Aurélian Miralles, Teddy Bruy, Katherine Wolcott, Mark D. Scherz, Dominik Begerow, Bank Beszteri, Michael Bonkowski, Janine Felden, Birgit Gemeinholzer, Frank Glaw, Frank Oliver Glöckner, Oliver Hawlitschek, Ivaylo Kostadinov, Tim W. Nattkemper, Christian Printzen, Jasmin Renz, Nataliya Rybalka, Marc Stadler, Tanja Weibulat, Thomas Wilke, Susanne S. Renner, Miguel Vences

Harold W. Manter Laboratory of Parasitology: Library Materials

Natural history collections are leading successful large-scale projects of specimen digitization (images, metadata, DNA barcodes), thereby transforming taxonomy into a big data science. Yet, little effort has been directed towards safeguarding and subsequently mobilizing the considerable amount of original data generated during the process of naming 15,000–20,000 species every year. From the perspective of alpha-taxonomists, we provide a review of the properties and diversity of taxonomic data, assess their volume and use, and establish criteria for optimizing data repositories. We surveyed 4,113 alpha-taxonomic studies in representative journals for 2002, 2010, and 2018, and found an increasing yet comparatively limited use …

Elucidation Of Molecular Links Between Obesity And Cancer Through Microrna Regulation, Haluk Dogan, Jiang Shu, Zeynep M. Hakguder, Zheng Xu, Juan Cui

School of Computing: Faculty Publications

Background: Obesity contributes to high cancer risk in humans and the mechanistic links between these two pathologies are not yet understood. Recent emerging evidence has associated obesity and cancer with metabolic abnormalities and inflammation where microRNA regulation has a strong implication. Methods: Inthisstudy,wehavedevelopedanintegratedframeworktounravelobesity-cancerlinkagefroma microRNA regulation perspective. Different from traditional means of identifying static microRNA targets based on sequence and structure properties, our approach focused on the discovery of context-dependent microRNA-mRNA interactions that are potentially associated with disease progression via large-scale genomic analysis. Specifically, a meta-regression analysis and the integration of multi-omics information from obesity and cancers were presented to investigate …

Comparative Evaluation Of Machine Learning Models For Groundwater Quality Assessment, Shine Bedi, Ashok Samal, Chittaranjan Ray, Daniel D. Snow

School of Computing: Faculty Publications

Contamination from pesticides and nitrate in groundwater is a significant threat to water quality in general and agriculturally intensive regions in particular. Three widely used machine learning models, namely, artificial neural networks (ANN), support vector machines (SVM), and extreme gradient boosting (XGB), were evaluated for their efficacy in predicting contamination levels using sparse data with non-linear relationships. The predictive ability of the models was assessed using a dataset consisting of 303 wells across 12 Midwestern states in the USA. Multiple hydrogeologic, water quality, and land use features were chosen as the independent variables, and classes were based on measured concentration …

Assessing The Hierarchy Of Long-Term Environmental Controls On Diatom Communities Of Yellowstone National Park Using Lacustrine Sediment Records, Victoria Chraibi, Sherilyn C. Fritz

Department of Earth and Atmospheric Sciences: Faculty Publications

An ecosystem’s ability to maintain structure and function following disturbance, defined as resilience, is influenced by a hierarchy of environmental controls, including climate, surface cover, and ecological relationships that shape biological community composition and productivity. This study examined lacustrine sediment records of naturally fishless lakes in Yellowstone National Park to reconstruct the response of aquatic communities to climate and trophic cascades from fish stocking. Sediment records of diatom algae did not exhibit a distinct response to fish stocking in terms of assemblage or algal productivity. Instead, 3 of 4 lakes underwent a shift to dominance by benthic diatom species from …

Spatial And Temporal Ecological Uniqueness Of Andean Diatom Communities Are Correlated With Climate, Geodiversity And Long-Term Limnological Change, Xavier Benito, Annika Vilmi, Melina Luethje, Maria Laura Carrevedo, Marja Lindholm, Sherilyn C. Fritz

Department of Earth and Atmospheric Sciences: Faculty Publications

High-elevation tropical lakes are excellent sentinels of global change impacts, such as climate warming, land-use change, and atmospheric deposition. These effects are often correlated with temporal and spatial beta diversity patterns, with some local communities contributing more than others, a phenomenon known as local contribution to beta diversity (LCBD) or ecological uniqueness. Microorganisms, such as diatoms, are considered whole-ecosystem indicators, but little is known about their sensitivity and specificity in beta diversity studies mostly because of the lack of large spatial and temporal datasets. To fill this gap, we used a tropical South American diatom database comprising modern (144 lakes) …

The Influence Of Isolated Thunderstorms And The Low-Level Wind Field On Nocturnally Migrating Birds In Central North America, Matthew S. Van Den Broeke, Timothy J. Gunkel

Department of Earth and Atmospheric Sciences: Faculty Publications

Seasonal bird migration occurs on large spatial scales and is influenced by many factors including weather conditions. Weather can include thunderstorms, which may force migrants to land or cause them to reroute a migration path. In this study, a sample of isolated thunderstorms was analyzed from the domains of three weather radars in the central United States to test hypotheses regarding the influence of thunderstorms on the distribution of migrants. Migrating bird density was often reduced in the wake of storms, and this wake reduction was typically more pronounced for larger, more intense and faster- moving storms, particularly in eastern …

The Influence Of Isolated Thunderstorms And The Low-Level Wind Field On Nocturnally Migrating Birds In Central North America, Matthew V.D. Broeke, Timothy J. Gunkel

Department of Earth and Atmospheric Sciences: Faculty Publications

Seasonal bird migration occurs on large spatial scales and is influenced by many factors including weather conditions. Weather can include thunderstorms, which may force migrants to land or cause them to reroute a migration path. In this study, a sample of isolated thunderstorms was analyzed from the domains of three weather radars in the central United States to test hypotheses regarding the influence of thunderstorms on the distribution of migrants. Migrating bird density was often reduced in the wake of storms, and this wake reduction was typically more pronounced for larger, more intense and faster- moving storms, particularly in eastern …

Controls On Channel Deposits Of Highly Variable Rivers: Comparing Hydrology And Event Deposits In The Burdekin River, Australia, Jan Alexander, Christopher M. Herbert, Christopher R. Fielding, Kathryn J. Amos

Department of Earth and Atmospheric Sciences: Faculty Publications

Discharge event frequency, magnitude and duration all control river chan- nel morphology and sedimentary architecture. Uncertainty persists as to whether alluvial deposits in the rock record are a time-averaged amalgam from all discharge events, or a biased record of larger events. This paper investigates the controls on channel deposit character and subsurface strati- graphic architecture in a river with seasonal discharge and very high inter- annual variability, the Burdekin River of north-east Australia. In such rivers, most sediment movement is restricted to a few days each year and at other times little sediment moves. However, the maximum discharge magnitude does …

End-Permian (252 Mya) Deforestation, Wildfires And Flooding—An Ancient Biotic Crisis With Lessons For The Present, Vivi Vajda, Stephen Mcloughlin, Chris Mays, Tracy D. Frank, Christopher R. Fielding, Allen Tevyaw, Veiko Lehsten, Malcolm Bocking, Robert S. Nicoll

Department of Earth and Atmospheric Sciences: Faculty Publications

Current large-scale deforestation poses a threat to ecosystems globally, and imposes substantial and prolonged changes on the hydrological and carbon cycles. The tropical forests of the Amazon and Indonesia are currently undergoing deforestation with catastrophic ecological consequences but widespread deforestation events have occurred several times in Earth’s history and these provide lessons for the future. The end-Permian mass-extinction event (EPE; ∼252 Ma) provides a global, deep-time analogue for modern deforestation and diversity loss. We undertook centimeter-resolution palynological, sedimentological, carbon stable-isotope and paleobotanical investigations of strata spanning the end- Permian event at the Frazer Beach and Snapper Point localities, in the …

Far-Field Transient Absorption Nanoscopy With Sub-50 Nm Optical Super-Resolution, Yali Bi, Chi Yang, Lei Tong, Haozheng Li, Boyu Yu, Shuai Yan, Guang Yang, Meng Deng, Yi Wang, Wei Bao, Lei Ye, Ping Wang

Department of Electrical and Computer Engineering: Faculty Publications

Nanoscopic imaging or characterizing is the mainstay of the development of advanced materials. Despite great progress in electronic and atomic force microscopies, label-free and far-field characterization of materials with deep sub- wavelength spatial resolution has long been highly desired. Herein, we demonstrate far-field super-resolution transient absorption (TA) imaging of two-dimensional material with a spatial resolution of sub-50 nm. By introducing a donut- shaped blue saturation laser, we effectively suppress the TA transition driven by near-infrared (NIR) pump–probe photons, and push the NIR-TA microscopy to sub-diffraction-limited resolution. Specifically, we demonstrate that our method can image the individual nano-grains in graphene with …

Precursor-Surface Interactions Revealed During Plasma-Enhanced Atomic Layer Deposition Of Metal Oxide Thin Films By In-Situ Spectroscopic Ellipsometry, Ufuk Kilic, Alyssa Mock, Derek Sekora, Simeon Gilbert, Shah R. Valloppilly, Natale J. Ianno, Marjorie Langell, Eva Schubert, Mathias Schubert

Department of Electrical and Computer Engineering: Faculty Publications

We find that a five-phase (substrate, mixed native oxide and roughness interface layer, metal oxide thin film layer, surface ligand layer, ambient) model with two-dynamic (metal oxide thin film layer thickness and surface ligand layer void fraction) parameters (dynamic dual box model) is sufficient to explain in-situ spectroscopic ellipsometry data measured within and across multiple cycles during plasma-enhanced atomic layer deposition of metal oxide thin films. We demonstrate our dynamic dual box model for analysis of in-situ spectroscopic ellipsometry data in the photon energy range of 0.7–3.4 eV measured with time resolution of few seconds over large numbers of cycles …

Transcriptional Analysis Of Cleft Palate In Tgfβ3 Mutant Mice, J. Liu, S.K. Chanumolu, K.M. White, M. Albahrani, Hasan H. Otu, A. Nawshad

Department of Electrical and Computer Engineering: Faculty Publications

Cleft palate (CP) is one of the most common craniofacial birth defects, impacting about 1 in 800 births in the USA. Tgf-β3 plays a critical role in regulating murine palate development, and Tgf-β3 null mutants develop cleft palate with 100% penetrance. In this study, we compared global palatal transcriptomes of wild type (WT) and Tgf-β3 −/− homozygous (HM) mouse embryos at the crucial palatogenesis stages of E14.5, and E16.5, using RNA-seq data. We found 1,809 and 2,127 differentially expressed genes at E16.5 vs. E14.5 in the WT and HM groups, respectively (adjusted p < 0.05; |fold change|> 2.0). We focused on the genes that …

Ecosystem-Specific Growth Responses To Climate Pattern By A Temperate Freshwater Fish, Jonathan Spurgeon, Mark A. Pegg, Kevin L. Pope, Lin Xie

Department of Finance: Faculty Publications

Somatic growth patterns among animal populations are maintained through complex processes that vary among ecosystems. Changes in growth patterns may be concomitant with changes in climate; however, understanding how growth will manifest among ecosystems is limited. Information embedded within fish hard-parts (i.e., otoliths, spines, vertebrae) can account for variation in growth patterns resulting from changing climate conditions. Channel catfish Ictalurus punctatus is a freshwater fish species widely distributed across North America with limited information regarding climate influences on growth and differences in climate-growth relations among ecological systems. We assessed growth (total length) response to changing climate conditions for channel catfish …

Resilience Management For Conservation Of Inland Recreational Fisheries, Edward V. Camp, Mark A. Kaemingk, Robert N. M. Ahrens, Warren M. Potts, William E. Pine Iii, Olaf L. F. Weyl, Kevin L. Pope

Department of Finance: Faculty Publications

Resilience thinking has generated much interest among scientific communities, yet most resilience concepts have not materialized into management applications. We believe that using resilience concepts to characterize systems and the social and ecological processes affecting them is a way to integrate resilience into better management decisions. This situation is exemplified by inland recreational fisheries, which represent complex socioecological systems that face unpredictable and unavoidable change. Making management decisions in the context of resilience is increasingly important given mounting environmental and anthropogenic perturbations to inland systems. Herein, we propose a framework that allows resilience concepts to be better incorporated into management …

Coerced Regimes: Management Challenges In The Anthropocene, David G. Angeler, Brian C. Chaffin, Shana M. Sundstrom, Ahjond S. Garmestani, Kevin L. Pope, Daniel R. Uden, Dirac Twidwell, Craig R. Allen

Department of Finance: Faculty Publications

Management frequently creates system conditions that poorly mimic the conditions of a desirable self-organizing regime. Such management is ubiquitous across complex systems of people and nature and will likely intensify as these systems face rapid change. However, it is highly uncertain whether the costs (unintended consequences, including negative side effects) of management but also social dynamics can eventually outweigh benefits in the long term. We introduce the term “coerced regime” to conceptualize this management form and tie it into resilience theory. The concept encompasses proactive and reactive management to maintain desirable and mitigate undesirable regime conditions, respectively. A coerced regime …

Communication Accommodation And Identity Gaps As Predictors Of Relational Solidarity In Interfaith Family Relationships, Toni Morgan, Jordan Soliz, Mackensie Minniear, Gretchen Bergquist

Department of Communication Studies: Faculty Publications

Guided by Communication Accommodation Theory (CAT) and Communication Theory of Identity (CTI), the purpose of this study was to investigate how families communicatively negotiate religious differences and how that negotiation is related to parent-child relational solidarity. Specifically, we examined the direct effects of (non)accommodative communication on relational solidarity and indirect effects via identity gaps. Using a cross-sectional survey from emerging adult college students (N = 234), we found nonaccommodative communication is indirectly related to lower relational solidarity through increased identity gaps. Accommodative communication is indirectly related to higher relational solidarity through decreased identity gaps. When parents use accommodative strategies, they …

Proceedings Of The 2019 American Bee Research Conference, Michael Simone-Finstrom, Elina L. Niño, Michelle L. Flenniken, Hanna Arrowsmith, Judy Wu-Smart

Department of Entomology: Faculty Publications

The 2019 American Bee Research Conference (ABRC) was held January 10–12, 2019 in conjunction with the annual convention of the American Honey Producers Association in Tempe, AZ. Over the three-day conference, a total of 45 oral presentations and 13 poster presentations were given, representing work done from over 27 institutions and 34 different research groups from throughout the United States and Canada. This proceedings contains and overview of the conference and the submitted abstracts for presentations given at the 2018 American Bee Research Conference.

Control Of Western Corn Rootworm Via Rnai Traits In Maize: Lethal And Sublethal Effects Of Sec23 Dsrna, Ana Maria Vélez, Elane Fishilevich, Murugesan Rangasamy, Chitvan Khajuria, David Mccaskill, Adriano E. Pereira, Premchand Gandra, Meghan L.F. Frey, Sarah Worden, Shannon Whitlock, Wendy Lo, Karl D. Schnelle, Jamie R, Lutz, Kenneth E. Narva, Blair Siegfried

Department of Entomology: Faculty Publications

Background: RNA interference (RNAi) triggered by maize plants expressing RNA hairpins against specific western corn rootworm ( WCR) transcripts have proven to be effective at controlling this pest. To provide robust crop protection, mRNA transcripts targeted by double-stranded RNA must be sensitive to knockdown and encode essential proteins.

Results: Using WCR adult feeding assays, we identified Sec23 as a highly lethal RNAi target. Sec23 encodes a coatomer protein, a component of the coat protein

(COPII) complex that mediates ER-Golgi transport. The lethality detected in WCR adults was also observed in early instar larvae, the life stage causing most …

Posters As An Effective Assessment Tool For A Capstone Course, David Gosselin, Doug Golick

Department of Entomology: Faculty Publications

A rubric was developed to assess student posters as a mechanism to evaluate learning outcomes for a senior capstone course. The analytic rubric allows for the efficient and systematic collection of data from posters by students who worked across a variety of disciplines including the physical, biological, Earth sciences, social science, and the humanities. The rubric effectively addressed a fundamental assumption and requirement put forth during rubric development, that is, it needs to be relatively easy to use without training while at the same time producing consistent results across evaluators. The overall Chronbach’s alpha of 0.80 across semesters indicates acceptable …

Integrated Crop–Livestock Systems In Paddy Fields: New Strategies For Flooded Rice Nutrition, Luiz Gustavo De O. Denardin, Amanda P. Martins, Felipe De C. Carmona, Murilo G. Veloso, Gabriela I. Carmona, Paulo César De F. Carvalho, Ibanor Anghinoni

Department of Entomology: Faculty Publications

Integrated crop–livestock systems (ICLSs) appear as a good alternative to increase nutrient use efficiency (NUE) in rice (Oryza sativa L.) through the improvement in nutrient cycling and soil chemical attributes in paddy fields. The objective of this study was to evaluate the impact of an ICLS on soil chemical attributes and on the fertilization requirement of N, P, and K by flooded rice in the Brazilian subtropical region. Nutritional status, yield, and NUE of flooded rice were evaluated by fertilization trials through rice response to different fertilization rates of N, P, and K. Soil chemical attributes were evaluated at …

Diabrotica Undecimpunctata Virus 2, A Novel Small Rna Virus Discovered From Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi Barber (Coleoptera: Chrysomelidae), Sijun Liu, Arnubio Valencia-Jiménez, Molly Darlington, Ana M. Vélez, Bryony C. Bonning

Department of Entomology: Faculty Publications

The genome of Diabrotica undecimpunctata virus 2 (DuV2), a positive-sense, single-stranded RNA virus identified from the southern corn root- worm transcriptome, comprises 5,313 nucleotides, including a short poly(A) tail. The two open reading frames encode a nonstructural polyprotein (p156) and a putative capsid protein (p25).

An Updated Genetic Marker For Detection Of Lake Sinai Virus And Metagenetic Applications, Deborah D. Iwanowicz, Judy Wu-Smart, Tugce Olgun, Autumn H. Smart, Clint R.V. Otto, Dawn Lopez, Jay D. Evans, Robert Cornman

Department of Entomology: Faculty Publications

Background. Lake Sinai Viruses (LSV) are common RNA viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade.

Methods. Here we report a new polymerase chain reaction (PCR) assay that is compatible with currently known lineages with …