Digital Commons Network^™

Enhanced Metabolism And Selection Of Pyrethroid-Resistant Western Corn Rootworms (Diabrotica Virgifera Virgifera Leconte), Dariane Souza, Arnubio V. Jiménez, Gautam Sarath, Lance J. Meinke, Nicholas J. Miller, Blair D. Siegfried

Department of Entomology: Faculty Publications

Western corn rootworm (WCR) pyrethroid resistance has been previously reported in the United States (US) western Corn Belt, and cross-resistance and synergism studies suggested that both target site insensitivity and enhanced metabolism may be conferring WCR resistance to pyrethroids. The present study aimed to investigate the potential mechanisms of WCR pyrethroid resistance and to estimate the heritability of the resistance trait. Biochemical assays using model substrates and spectrophotometry revealed 2–4-fold higher activity of P450s and esterases in pyrethroid-resistant WCR populations, whereas the biological activity of glutathione S-trans- ferase was similar between populations tested. No mutation in the voltage-gated sodium channel …

Differential Defense Responses Of Upland And Lowland Switchgrass Cultivars To A Cereal Aphid Pest, Lise Pingault, Nathan A. Palmer, Kyle G. Koch, Tiffany Heng-Moss, Jeff Bradshaw, Javier Seravalli, Paul G. Twigg, Joe Louis, Gautam Sarath

Department of Entomology: Faculty Publications

Yellow sugarcane aphid (YSA) (Sipha flava, Forbes) is a damaging pest on many grasses. Switchgrass (Panicum virgatum L.), a perennial C4 grass, has been selected as a bioenergy feedstock because of its perceived resilience to abiotic and biotic stresses. Aphid infestation on switchgrass has the potential to reduce the yields and biomass quantity. Here, the global defense response of switchgrass cultivars Summer and Kanlow to YSA feeding was analyzed by RNA-seq and metabolite analysis at 5, 10, and 15 days after infestation. Genes upregulated by infestation were more common in both cultivars compared to downregulated genes. In total, a higher …

Molecular Gut-Content Analysis Of Adult Ground Beetles (Coleoptera: Carabidae) Provides No Evidence Of Predation Of Western Corn Rootworm (Coleoptera: Chrysomelidae) In A Nebraska Corn Agroecosystem, Camila Oliveira-Hofman, Vinícius S. Victor, Lance Meinke, Julie A. Peterson

Department of Entomology: Faculty Publications

This study was conducted to characterize the ground beetle (Coleoptera: Carabidae) community in Nebraska continuous cornfields and investigate the potential for predation of the western corn rootworm, Diabrotica virgifera virgifera LeConte, a key rootfeeding insect pest that is an annual management challenge in this system. Seven collection dates were conducted at five commercial cornfields in west central Nebraska during the growing season of 2014. In each field, carabids were sampled using five 24-h pitfall traps. Carabid specimens were placed in 95% ethanol in the field and stored at –20 °C to preserve DNA. After identification to the species level, DNA …

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting In Cd8+ T Cell Memory And Delayed Tumor Progression, Ross Darling, Sujata Senapati, John Christiansen, Luman Liu, Amanda E. Ramer-Tati, Balaji Narasimhan, Michael Wannemuehler

Department of Food Science and Technology: Faculty Publications

Introduction: Adjuvants and immunotherapies designed to activate adaptive immunity to eliminate infectious disease and tumors have become an area of interest aimed at providing a safe and effective strategy to prevent or eliminate disease. Existing approaches would benefit from the development of immunization regimens capable of inducing efficacious cell- mediated immunity directed toward CD8+ T cell-specific antigens. This goal is critically dependent upon appropriate activation of antigen-presenting cells (APCs) most notably dendritic cells (DCs). In this regard, polyanhydride particles have been shown to be effectively internalized by APCs and induce activation.

Methods: Here, a prophylactic vaccine regimen designed as a …

The Trait Repertoire Enabling Cyanobacteria To Bloom Assessed Through Comparative Genomic Complexity And Metatranscriptomics, Huansheng Cao, Yohei Shimura, Morgan M. Steffen, Zhou Yang, Jingrang Lu, Allen Joel, Landon Jenkins, Masanobu Kawachi, Yabin Yin, Ferran Garcia-Pichel

Department of Food Science and Technology: Faculty Publications

Water bloom development due to eutrophication constitutes a case of niche specialization among planktonic cyanobacteria, but the genomic repertoire allowing bloom formation in only some species has not been fully characterized. We posited that the habitat relevance of a trait begets its underlying genomic complexity, so that traits within the repertoire would be differentially more complex in species successfully thriving in that habitat than in close species that cannot. To test this for the case of bloom-forming cyanobacteria, we curated 17 potentially relevant query metabolic pathways and five core pathways selected according to existing ecophysiological literature. The available 113 genomes …

Desiccation Tolerance In Streptophyte Algae And The Algae To Land Plant Transition: Evolution Of Lea And Mip Protein Families Within The Viridiplantae, Burkhard Becker, Xuehuan Feng, Yanbin Yin, Andreas Holzinger

Department of Food Science and Technology: Faculty Publications

The present review summarizes the effects of desiccation in streptophyte green algae, as numerous experimental studies have been performed over the past decade particularly in the early branching streptophyte Klebsormidium sp. and the late branching Zygnema circumcarinatum. The latter genus gives its name to the Zygenmatophyceae, the sister group to land plants. For both organisms, transcriptomic investigations of desiccation stress are available, and illustrate a high variability in the stress response depending on the conditions and the strains used. However, overall, the responses of both organisms to desiccation stress are very similar to that of land plants. We highlight the …

Tdt Sandwich: An Open Source Dry Heat System For Characterizing The Thermal Resistance Of Microorganisms, Soon Kiat Lau, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

The determination of the thermal death kinetics of microorganisms has traditionally been performed with liquid baths which have some disadvantages such as liquid spillage and liquid infiltration into samples. The TDT Sandwich was developed as a free, open source alternative that utilizes dry heat. The system is capable of heating samples up to 140C and maintaining it within 0.2C of the target temperature. Other features of the TDT Sandwich include adjustable heating rates up to approximately 100C/min, real-time display and recording of temperature readings at a nominal rate of 5 Hz, an optional thermocouple for acquiring temperature of samples, built-in …

Humidosh: A Self-Contained Environmental Chamber With Controls For Relative Humidity And Fan Speed, Soon Kiat Lau, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

Relative humidity can affect physical, biological, and chemical changes in biological samples through modification of water activity and is also known to be important in the fabrication of sensitive electronic devices. The HumidOSH is a free, open source, and self-contained system for creating a controlled relative humidity environment within the range of 3 to 97% with a 0.2% tolerance. Each HumidOSH unit also comes with a fan with adjustable fan rotational speed to improve moisture uniformity inside the chamber. The system includes many additional features such as glove ports for manipulating samples, a sample door for transferring objects in and …

A High-Throughput Lc-Ms/Ms Method For The Measurement Of The Bile Acid/Salt Content In Microbiome-Derived Sample Sets, Thomas D. Horvath, Sigmund J. Haidacher, Kathleen M. Hoch, Jennifer M. Auchtung, Anthony M. Haag

Department of Food Science and Technology: Faculty Publications

Due to the physicochemical properties of bile acids/salts (i.e., hydrophobic and ionizable), the application of reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods are ideally suited for the measurement of these compounds in a host of microbiologically-relevant matrices. Here, we provide a detailed bioanalytical protocol that contains several modifications of a method previously described by Wegner et al. [1]. Briefly, this modified method exhibits the following advantages for the measurement of cholic acid (CA), taurocholic acid (TCA), and deoxycholic acid (DCA) in microbiome-relevant sample matrices: i) fecal sample processing has been streamlined by the elimination of lyophilization and manual homogenization steps; …

Phytochemical Characterization Of Ultrasound-Processed Sorghum Sprouts For The Use In Functional Foods, Sadia Hassan, Muhammad Imran, Muhammad Haseeb Ahmad, Muhammad Imran Khan, Changmou Xu, Muhammad Kamran Khan, Niaz Muhammad

Department of Food Science and Technology: Faculty Publications

In the recent years, application of ultrasound has been taken into considera- tion to enhance the nutritive value of processed food products, either by retaining or modulating their phytochemical components. Profile of phyto- chemicals in sorghum seeds was amended by optimizing ultrasound appli- cation for their use as sprouts. In this study, overall impact on various phytochemical constituents (alkaloids, phytates, saponins, and sterols), radi- cal scavenging activity (2,2-diphenyl-1-picrylhydrazyl assay, ferric reducing antioxidant power assay, and oxygen radical absorbance capacity assay assay), phenolic profile (total phenolics content, total flavonoids content, ferulic acid, gallic acid, catechin, quercetin, and tannin) along with in …

Fecal Short-Chain Fatty Acid Concentrations Increase In Newly Paired Male Marmosets (Callithrix Jacchus), Lifeng Zhu, Mallory J. Suhr Van Haute, Haley R. Hassenstab, Caroline Smith, Devin J. Rose, Aaryn C. Must, Andrew K. Benson, Jeffrey A. French

Department of Food Science and Technology: Faculty Publications

The role by which the gut microbiome influences host health (e.g., energy equilibrium and immune system) may be partly mediated by short-chain fatty acids, which are bacterial fermentation products from the dietary fibers. However, little is known about longitudinal changes in gut microbiome metabolites during cohabitation alongside social contact. In common marmosets (Callithrix jacchus), the gut microbiome community is influenced by social contact, as newly paired males and females develop convergent microbial profiles. Here, we monitored the dynamics of short-chain fatty acid concentrations in common marmoset feces from the prepairing (PRE) to postpairing (POST) stages. In males, we observed that …

Wild Primate Microbiomes Prevent Weight Gain In Germ-Free Mice, Dimitrios N. Sidiropoulos, Gabriel A. Al-Ghalith, Robin R. Shields-Cutler, Tonya L. Ward, Abigail J. Johnson, Pajau Vangay, Dan Knights, Purna C. Kashyap, Yibo Xian, Amanda E. Ramer-Tait, Jonathan B. Clayton

Department of Food Science and Technology: Faculty Publications

Background: The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly industrialized countries. At the same time, Westernized diets low in dietary fiber have been shown to cause loss of gut microbial diversity. However, the link between microbiome composition, loss of dietary fiber, and obesity has not been well defined.

Results: To study the interactions between gut microbiota, dietary fiber, and weight gain, we transplanted captive and …

Mucosa-Associated Microbiota In Barrett’S Esophagus, Dysplasia, And Esophageal Adenocarcinoma Differ Similarly Compared With Healthy Controls, Shajan Peter, Amanda Pendergraft,, William Vanderpol, Mel Wilcox, Kondal R. Kyanam Kabir Baig, Casey Morrow, Jacques Izard, Peter J. Mannon

Department of Food Science and Technology: Faculty Publications

Introduction: Alterations in the composition of the human gut microbiome and its metabolites have been linked to gut epithelial neoplasia. We hypothesized that differences in mucosa-adherent Barrett’s microbiota could link to risk factors, providing risk of progression to neoplasia.

Methods: Paired biopsies from both diseased and nonaffected esophagus (as well as gastric cardia and gastric juice for comparison) from patients with intestinal metaplasia (n = 10), low grade dysplasia (n = 10), high grade dysplasia (n = 10), esophageal adenocarcinoma (n = 12), and controls (n = 10) were processed for mucosa-associated bacteria …

Expression Profiling Of Human Milk Derived Exosomal Micrornas And Their Targets In Hiv‐1 Infected Mothers, Muhammad Atif Zahoor, Mcmaster University, Hamilton, Bethany M. Henrick, Chris P. Verschoor, Alash’Le Abimiku, Sophia Osawe, Kenneth L. Rosenthal

Department of Food Science and Technology: Faculty Publications

Despite the use of antiretroviral therapy (ART) in HIV‐1 infected mothers approximately 5% of new HIV‐1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV‐1 infections in infants. Human milk (HM) exosomes are highly enriched in microRNAs (miRNAs), which play an important role in neonatal immunity. Furthermore, HM exosomes from healthy donors are known to inhibit HIV‐1 infection and transmission; however, the effect of HIV‐1 on HM exosomal miRNA signatures remains unknown. In this study, we used nCounter NanoString technology and investigated miRNAs expression profiles in first week postpartum HM …

The International Scientific Association For Probiotics And Prebiotics (Isapp) Consensus Statement On The Definition And Scope Of Synbiotics, Kelly S. Swanson, Glenn R. Gibson, Robert W. Hutkins, Raylene A. Reimer, Gregor Reid, Kristin Verbeke, Karen P. Scott, Hannah D. Holscher, Meghan B. Azad, Nathalie M. Delzenne, Mary Ellen Sanders

Department of Food Science and Technology: Faculty Publications

In May 2019, the International Scientific Association for Probiotics and Prebiotics (ISAPP) convened a panel of nutritionists, physiologists and microbiologists to review the definition and scope of synbiotics. The panel updated the definition of a synbiotic to “a mixture comprising live microorganisms and substrate(s) selectively utilized by host microorganisms that confers a health benefit on the host”. The panel concluded that defining synbiotics as simply a mixture of probiotics and prebiotics could suppress the innovation of synbiotics that are designed to function cooperatively. Requiring that each component must meet the evidence and dose requirements for probiotics and prebiotics individually could …

Effect Of Ph And Heat Treatment On The Antioxidant Activity Of Egg White Protein-Derived Peptides After Simulated In-Vitro Gastrointestinal Digestion, Priyanka Singh Rao, Emerson Nolasco, Akihiro Handa, Michael J. Naldrett, Sophie Alvarez, Kaustav Majumder

Department of Food Science and Technology: Faculty Publications

The study aimed to analyze pH and heat treatment’s effect in modulating the release of peptides with antioxidant activity after simulated gastrointestinal (GI) digestion of Egg white powder (EWP). EWP samples with neutral (EWPN) and alkaline (EWPA) pH were heat-treated at 20, 60, and 90 ◦C and analyzed for protein aggregation, solubility, and GI digestibility. Heat treatment decreased solubility and induced protein aggregation, which was higher for EWPN as compared to EWPA. The unfolding of EWPA proteins at 60 ◦C exhibited a higher GI digestibility and antioxidant activity via Oxygen Radical Absorbance Capacity (ORAC) assay as compared to EWPN. Interestingly, …

Comprehensive Identification And Analyses Of The Hsf Gene Family In The Whole-Genome Of Three Apiaceae Species, Qiaoying Pei, North China University Of Science And Technology, Tong Wu, Qihang Yang, Ke Gong, Rong Zhou, Chunlin Cui, Ying Yu, Wei Zhao, Xi Kang, Rui Cao, Xiaoming Song

Department of Food Science and Technology: Faculty Publications

Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops. Heat shock transcription factors ( Hsf ) play important roles in heat tolerance during plant development. Here, we conducted systematic analyses of the Hsf gene family in three Apiaceae species, including 17 Apium graveolens (celery), 32 Coriandrum sativum (coriander), and 14 Daucus carota (carrot). A total of 73 Hsf genes were identified in three representative species, including Arabidopsis thaliana , Vitis vinifera , and Lactuca sativa . Whole-genome duplication played important roles in the Hsf gene family’s expansion within Apiaceae. Interestingly, we found that coriander …

Polyphenolic Fractions Isolated From Red Raspberry Whole Fruit, Pulp, And Seed Differentially Alter The Gut Microbiota Of Mice With Diet-Induced Obesity, Yibo Xian, Rong Fan, Jing Shao, Ashley Mulcahy Toney, Soonkyu Chung, Amanda E. Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Polyphenol extracts from red raspberry (RR) whole fruit or pulp, but not seed, attenuate high-fat (HF) dietinduced obesity in mice. Because host metabolism is linked to the microbiota, we investigated the effects of polyphenols from RR fruit, pulp, and seed on the microbiome. RR polyphenols significantly decreased the abundance of specific taxa that were increased during HF feeding relative to a low-fat diet, including Ruminococcus and an unclassified genus from Clostridiales. Compared to a HF diet, pulp and seed polyphenols increased Roseburia abundance and decreased levels of an unclassified genus from Mogibacteriaceae. RR seed polyphenols uniquely increased Bifidobacterium compared …

Mothers’ And Fathers’ Self-Regulation Capacity, Dysfunctional Attributions And Hostile Parenting During Early Adolescence: A Process-Oriented Approach, Melissa L. Sturge-Apple, Zhi Li, Meredith Martin, Hannah R. Jones-Gordils, Patrick T. Davies

Department of Educational Psychology: Faculty Publications

The parent-child relationship undergoes substantial reorganization over the transition to adolescence. Navigating this change is a challenge for parents because teens desire more behavioral autonomy as well as input in decision-making processes. Although it has been demonstrated that changes in parental socialization approaches facilitates adolescent adjustment, very little work has been devoted to understanding the underlying mechanisms supporting parents’ abilities to adjust caregiving during this period. Guided by self-regulation models of parenting, the present study examined how parental physiological and cognitive regulatory capacities were associated with hostile and insensitive parent conflict behavior over time. From a process-oriented perspective, we tested …

Mothers’ And Fathers’ Self-Regulation Capacity, Dysfunctional Attributions And Hostile Parenting During Early Adolescence: A Process-Oriented Approach, Melissa L. Sturge-Apple, Zhi Li, Meredith J. Martin, Hannah R. Jones-Gordils, Patrick T. Davies

Department of Educational Psychology: Faculty Publications

The parent-child relationship undergoes substantial reorganization over the transition to adolescence. Navigating this change is a challenge for parents because teens desire more behavioral autonomy as well as input in decision-making processes. Although it has been demon- strated that changes in parental socialization approaches facilitates adolescent adjustment, very little work has been devoted to understand- ing the underlying mechanisms supporting parents’ abilities to adjust caregiving during this period. Guided by self-regulation models of parenting, the present study examined how parental physiological and cognitive regulatory capacities were associated with hostile and insen- sitive parent conflict behavior over time. From a process-oriented …

“Isn’T Atheism A White Thing?”: Centering The Voices Of Atheists Of Color, Dena M. Abbott, Debra Mollen, Caitlin Mercier, Elyxcus J. Anaya, Victoria A. Rukus

Department of Educational Psychology: Faculty Publications

Despite a general shift toward secularity, very few people of color in the United States identify as atheist. Further, atheists of color are underrepresented in studies of atheists, and the experiences of atheists of color specifically have, to date, not been captured in the extant scholarship. Addressing this gap in the literature, we interviewed 17 self-identified adult atheists of color, predominantly from Christian backgrounds, residing in the United States, using a critical feminist phenomenological approach. Six broad themes emerged from the data: (a) atheist identity development, (b) experiences of discrimination, (c) isolation, (d) violations of cultural expectations, (e) strategic outness, …

Electrospun Thymosin Beta-4 Loaded Plga/Pla Nanofiber/ Microfiber Hybrid Yarns For Tendon Tissue Engineering Application, Shaohua Wu, Rong Zhou, Fang Zhou, Philipp N. Streubel, Shaojuan Chen, Bin Duan

Department of Mechanical and Materials Engineering: Faculty Publications

Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (Tβ4) loaded PLGA/PLA …

Additive Manufacturing Of Magnesium Alloys, Rakeshkumar Karunakaran, Sam Ortgies, Ali Tamayol, Florin Bobaru, Michael P. Sealy

Department of Mechanical and Materials Engineering: Faculty Publications

Magnesium alloys are a promising new class of degradable biomaterials that have a similar stiffness to bone, which minimizes the harmful effects of stress shielding. Use of biodegradable magnesium implants eliminates the need for a second surgery for repair or removal. There is a growing interest to capitalize on additive manufacturing's unique design capabilities to advance the frontiers of medicine. However, magnesium alloys are difficult to 3D print due to the high chemical reactivity that poses a combustion risk. Furthermore, the low vaporization temperature of magnesium and common biocompatible alloying elements further increases the difficulty to print fully dense structures …

3d Printing Of Multilayered Scaffolds For Rotator Cuff Tendon Regeneration, Xiping Jiang, Shaohua Wu, Mitchell Kuss, Yunfan Kong, Wen Shi, Philipp N. Streubel, Tieshi Li, Bin Duan

Department of Mechanical and Materials Engineering: Faculty Publications

Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties, as well as controllable microenvironments for tendon regeneration. In this study, we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic-co-glycolic acid (PLGA) with cell-laden collagen-fibrin hydrogels. We designed and fabricated two types of scaffolds: one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole. Uniaxial tensile tests …

Facile Production Of Large-Area Cell Arrays Using Surface-Assembled Microdroplets, Karla Perez-Toralla, Angel Olivera-Torres, Mark A. Rose, Amir Monemian Monemianesfahani, Keerthana Reddy, Ruiguo Yang, Stephen Morin

Department of Mechanical and Materials Engineering: Faculty Publications

Techniques that enable the spatial arrangement of living cells into defined patterns are broadly applicable to tissue engineering, drug screening, and cell–cell investigations. Achieving large-scale patterning with single-cell resolution while minimizing cell stress/damage is, however, technically challenging using existing methods. Here, a facile and highly scalable technique for the rational design of reconfigurable arrays of cells is reported. Specifically, microdroplets of cell suspensions are assembled using stretchable surface-chemical patterns which, following incubation, yield ordered arrays of cells. The microdroplets are generated using a microfluidic- based aerosol spray nozzle that enables control of the volume/size of the droplets delivered to the …

Fluid Mechanics Education Using Japanese Anime: Examples From “Castle In The Sky” By Hayao Miyazaki, Sangjin Ryu, Haipeng Zhang, Markeya S. Peteranetz, Tareq Daher

Department of Mechanical and Materials Engineering: Faculty Publications

Current K-12 and undergraduate student have grown up with constant exposure to visual popular culture (e.g., movies, TV programs, graphic novels, etc.). Because youth find pop culture references in the classroom to be engaging, many science and engineering instructors have shown that examples found in visual pop culture can be used to teach science and engineering concepts.1,2 One recent study on the efficay os using pop ulture for instruction found that high school studetns learning from graphic-novel styles comics acquired deeper understanding of complex subject matter and reported higher engagement with the instructional materials as compared to students learning from …

Role Of Active Nanoliposomes In The Surface And Bulk Mechanical Properties Of Hybrid Hydrogels, R. Kadri, J. Bacharouch, K. Elkhoury, G. Ben Messaoud, C. Kahn, S. Desobry, M. Linder, Ali Tamayol, G. Francius, J.F. Mano, L. Sanchez-Gonzalez, E. Arab-Tehrany

Department of Mechanical and Materials Engineering: Faculty Publications

Nanoliposomes are widely used as delivery vehicles for active compounds. Nanoliposomes from rapeseed phospholipids were incorporated into interpenetrating polymer network hydrogels of gelatin methacryloyl and alginate. The multiscale physicochemical properties of the hydrogels are studied both on the surface and through the thickness of the 3D network. The obtained composite hydrogels exhibited strong mechanical properties and a highly porous surface. The blend ratio, as well as the concentration of nanoliposomes, affects the properties of the hydrogels. Nanofunctionalized hydrogels induced keratinocyte growth. These advantageous characteristics may open up many applications of the developed hydrogels in drug delivery and tissue engineering

Energy Development And Production In The Great Plains: Implications And Mitigation Opportunities, Jacqueline P. Ott, Bruce B. Hanberry, Mona Khalil, Mark W. Paschke, Max Post Van Der Burg, A.J. Prenni

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Energy is an integral part of society. The major US energy sources of fossil fuels (coal, oil, natural gas); biofuels (ethanol); and wind are concentrated in grassland ecosystems of the Great Plains. As energy de- mand continues to increase, mounting pressures will be placed on North American grassland systems. In this review, we present the ecological effects of energy development and production on grassland sys- tems. We then identify opportunities to mitigate these effects during the planning, construction, and pro- duction phases by using informed methodology and improved technology. Primary effects during energy development include small- and large-scale soil disturbance …

Temporospatial Shifts In Sandhill Crane Staging In The Central Platte River Valley In Response To Climatic Variation And Habitat Change, Andrew J. Caven, Emma M. Brinley Buckley, Kelsey C. King, Joshua Wiese, David M. Baasch, Greg D. Wright, Mary Harner, Aaron T. Pearse, Matt Raabe, Dana Varner, Brice Krohn, Nicole Arcilla, Kirk D. Schroeder, Kenneth F. Dinan

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Over 80% of the Mid-Continent Sandhill Crane (Antigone canadensis) Population (MCP), estimated at over 660,000 individuals, stops in the Central Platte River Valley (CPRV) during spring migration from mid-February through mid-April. Research suggests that the MCP may be shifting its distribution spatially and temporally within the CPRV. From 2002 to 2017, we conducted weekly aerial surveys of Sandhill Cranes staging in the CPRV to examine temporal and spatial trends in their abundance and distribution. Then, we used winter temperature and drought severity measures from key wintering and early migratory stopover locations to assess the impacts of weather patterns …

Hiv-1 Subtypes And Drug Resistance Mutations Among Female Sex Workers Varied In Different Cities And Regions Of The Democratic Republic Of Congo, Eun Hee Kwon, Godefroid M. A. Musema, Jessica Boelter, Sydney Townsend, Desire Tshala-Katumbay, Patrick K. Kayembe, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background Complex mosaic structures of HIV-1 were found in the Democratic Republic of Congo (DRC). Currently, there is limited information on the circulating HIV-1 strains, the distribution of these strains and antiretroviral (ART) resistant viruses in different regions of the country, and the HIV-1 strains harbored by the high-risk groups like female sex workers (FSW) reported to be the source of recombinant and ART resistant viruses.

Methods Dried Blood Spots (DBS), collected from 325 infected FSWs in ten cities from 2012 DRC HIV/STI Integrated Biological and Behavioral Surveillance Survey, were tested for HIV-1 genotypes and antiretroviral resistance mutations. Regional segregation …