Digital Commons Network^™

Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore

Nebraska Center for Virology: Faculty Publications

In vitro neurovirological studies of viral infectivity or viral gene expression may be confounded by the mulHple neural cell types and/or fibrob last contamination present in early passage cultures prepared from dissociated human central nervous system (eNS) tissue. We have developed highly enriched astrocyte cultures for neurovirological study by culturing in a serum-free defined medium, 816, supplemented with basic fibroblast growth factor (FGF-2). Subculture in this medium selects against fibroblast proliferation and favors sustained proliferation of a highly enriched glial fibrillary acidic protein (GFAP)-positive cell population. These astrocytes support productive replication of cytomegalovirus (CMV) and transient expression of transfected CMVand …

Hormone Concentrations In The Caudal Vena Cava During The First Ovarian Follicular Wave Of The Oestrous Cycle In Heifers, F. M. Rhodes, L. A. Fitzpatrick, K. W. Entwistle, J. E. Kinder

Department of Animal Science: Faculty Publications

Changes in pulsatile secretion of LH, FSH, oestradiol and progesterone were related to the growth phase, early plateau phase and regression phase of the first ovarian dominant follicle of the oestrous cycle in Bos indicus heifers. Relationships between these hormones during the three phases were also investigated. Accurate measurements of episodic ovarian steroid secretion were obtained by catheterizing the caudal vena cava via the lateral saphenous vein; the tip of the catheter was positioned just cranial to the ovarian vein using transrectal ultrasonography. Pulsatile secretion of oestradiol was increased only during the growth phase of the dominant follicle and was …

Collaborative Multimedia, Judy Diamond, Alan B. Bond, Beth Schenker, Debra Meier, Dana Twersky

Alan Bond Publications

Six natural history institutions contributed video and other images to produce a single multimedia exhibit about famous paleontology sites throughout the United States. In Mesozoic Monsters. Mammals and Magnolias users can view videos of the original excavation of each of the sites and also play computer games relating to each location. This project provides a model for how collaboration among museums can reduce the cost of multimedia exhibits while improving quality and making them available to wider audiences.

Melengestrol Acetate At Greater Doses Than Typically Used For Estrous Synchrony In Bovine Females Does Not Mimic Endogenous Progesterone In Regulation Of Secretion Of Luteinizing Hormone And 17Β-Estradiol, F. N. Kojima, J. R. Chenault, M. E. Wehrman, E. G. M. Bergfeld, Andrea S. Cupp, L. A. Werth, V. Mariscal, T. Sanchez, Roger J. Kittok, J. E. Kinder

Department of Animal Science: Faculty Publications

Our working hypothesis was that doses of melengestrol acetate (MGA) greater than those typically administered in estrous synchrony regimens would regulate secretion of LH and 17β-estradiol (E₂) as endogenous progesterone (P₄) does during the midluteal phase of the estrous cycle. We also hypothesized that endogenous P₄ from the CL would interact with MGA to further decrease the frequency of LH pulses and E₂. Cows on Day 5 of their estrous cycle (Day 0 = estrus) were randomly assigned to an untreated control group (CONT, n = 5) or to one of six MGA …

Ec95-1560 Use Of Cultural Practices In Crop Insect Pest Management, Robert J. Wright

University of Nebraska-Lincoln Extension: Historical Materials

This Extension Circular reviews what is known about the effects of rotations, tillage practices, and planting and harvest dates on crop insect management, focusing on major crops in Nebraska.

Before the development of synthetic organic insecticides (pre-DDT era), rotations, tillage practices, planting and harvest dates, and other nonchemical cultural controls were commonly recommended for insect management. Research focused on crop rotation and other cultural practices for insect management. With the development of DDT and later insecticides however, research on nonchemical controls decreased markedly.

With the emergence of the concept of sustainable agriculture in the 1980s, there has been increased emphasis …

Ec95-740 Pollution Prevention: A Tool Kit For Metal Finishers, Janet R. Hygnstrom, Wayne Woldt, Mohamed F. Dahab

University of Nebraska-Lincoln Extension: Historical Materials

Here's a Look at What's Ahead in the Pollution Prevention: A Tool Kit for Metal Finishers:

• Pollution Prevention Contacts for EPA Regions VII and VIII>
• Why the Concern About Waste?>
• Getting Off to a Good Start>
• Hazardous Waste>
• Material Safety Data Sheets>
• Material Safety Data Sheets — A Closer Look>
• Pollution Prevention Strategies for Your Business, Through Changes in: Housekeeping and Maintenance, Inventory, Basic Operations, Equipment, Raw Materials, Process Technology, Waste Exchanges and Recycling>
• Ready to Begin Preventing Pollution?>
• Yard Waste, A Growing Concern>
• Blow Your Horn! Publicize Your Pollution …

Ec95-739 Pollution Prevention: A Tool Kit For Farm Cooperatives, Janet R. Hygnstrom, Wayne Woldt, Mohamed F. Dahab

University of Nebraska-Lincoln Extension: Historical Materials

Would you like to:

• Reduced your operating costs by using fewer materials and supplies
• Reduce hazardous and nonhazardous waste transportation and disposal costs
• Reduce liability and risks associated with hazardous waste?
• Reduce the paperwork and record keeping requirements associated with hazardous waste?
• Improve workplace safety and employee health?
• Help safeguard the environment?
• Improve your company's image?
• Increase your company's business activities?

If you answered "Yes," to any of the above questions, you may want to look at the materials in this pollution prevention tool kit.

Ec95-743 Pollution Prevention: A Tool Kit For Autobody Repair Shops, Janet R. Hygnstrom, Wayne Woldt, Mohamed F. Dahab

University of Nebraska-Lincoln Extension: Historical Materials

Would you like to:

• Reduce your operating costs by using fewer materials and supplies?
• Reduce hazardous and nonhazardous waste transportation and disposal costs?
• Reduce liability and risks associated with hazardous waste?
• Reduce the paperwork and record keeping requirements associated with hazardous waste?
• Improve workplace safety and employee health?
• Help safeguard the environment?
• Improve your company's image?
• Increase your company's business activities?

If you answered "Yes," to any of the above questions, you may want to look at the materials in this pollution prevention tool kit.

Ec95-742 Pollution Prevention: A Tool Kit For Vehicle Maintenance Shops, Janet R. Hygnstrom, Wayne Woldt, Mohamed F. Dahab

University of Nebraska-Lincoln Extension: Historical Materials

Would you like to:

• Reduce your operating costs by using fewer materials and supplies?
• Reduce hazardous and nonhazardous waste transportation and disposal costs?
• Reduce liability and risks associated with hazardous waste?
• Reduce the paperwork and record keeping requirements associated with hazardous waste?
• Improve workplace safety and employee health?
• Help safeguard the environment?
• Improve your company's image?
• Increase your company's business activities?

If you answered "Yes," to any of the above questions, you may want to look at the materials in this pollution prevention tool kit.

Nf95-218 Conjunctive Use Policy Options, William Miller

University of Nebraska-Lincoln Extension: Historical Materials

Background

The hydrologic cycle controls water available for use. Rainfall, evaporation, runoff, percolation, and transpiration combine to influence the water available at a specific location at a particular point in time. The speed at which water moves among stages in the hydrologic cycle and the amount of time it spends in storage at any stage affects water availability to users. The conjunctive use issue refers to the portion of the hydrologic cycle where groundwater and surface water interface and influence each other.

Users who couldn't depend on an irregular flow of water over time developed systems that modify the variable …

13.1.8. Life History And Management Of The Blue-Winged Teal, James H. Gammonley, Leigh H. Fredrickson

Waterfowl Management Handbook

The blue-winged teal is a small dabbling duck that is common in North America and northern South America. The species is highly mobile and has an opportunistic life history strategy. Breeding populations respond to variable wetland conditions in the drought-prone prairie regions of the north-central United States and southern Canada. Extensive habitat loss and degradation has occurred on the prairies and on neotropical wintering areas in recent decades. Renewed interest in the ecology and management of blue-winged teal has resulted from these environmental pressures. We review life history characteristics of blue-winged teal that are important to managers. Readers should consult …

Canadian Cooperative Wildlife Health Centre, Volume 3-3, Winter 1995

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Loon Survey to Continue in 1996 on a Fully National Scale
Meet More CCWHC Staff
Waterfowl Die-off in Mexico
Newcastle Disease in Double-crested Cormorants - Summer 1995
The Northward March of Raccoon Rabies - Update
Rabbit Viral Hemorrhagic Disease on the Loose in Australia
Verminous pneumonia in red foxes
Common Loons: Complex Causes of Mortality
Parvovirus in Raccoons
Giardia in Voles
Beluga Whales in the St Lawrence Estuary
Insecticide Poisoning in Robins
Emaciated Great Horned Owls
Parvovirus and Trichinosis in Urban Raccoons
Botulism At Pakowki Lake, 1995
The Case of the Killer Cookies: Apparent Chocolate Poisoning of Gulls
Unusual Mortality …

Nf95-212 Supporting Stepfamilies, Tana Seymour, Connie Francis, Pat Steffens

University of Nebraska-Lincoln Extension: Historical Materials

This NebFact is the first in a series of NebFacts developed to support and strengthen stepfamilies.

Nf95-223 Supporting Stepfamilies: What Do The Children Feel?, Tana Seymour, Connie Francis, Patricia Steffens

University of Nebraska-Lincoln Extension: Historical Materials

This NebFact is part of a series about the special circumstances of stepfamilies.

Ec95-1511-D Insect Management Guide For Nebraska Alfalfa, Soybeans, Wheat, Range And Pasture, S. D. Danielson, R. J. Wright, J. F. Witkowski

University of Nebraska-Lincoln Extension: Historical Materials

EC 95-1511-D: This circular is a guide for the insects which plague the alfalfa, soybeans, wheat, range and pasture grown/used in Nebraska. The circular details the common insects of the different crops and how they should best be managed by farmers and ranchers.

Specificity Of The Red Imported Fire Ant Phagostimulant Response To Carbohydrates, R. K. Vander Meer, C. S. Lofgren, J. A. Seawright

Entomology Papers from Other Sources

The red imported fire ant, Solenopsis invicta Buren, is considered an oil-loving feeder, however, carbohydrates are essential ingredients in the diet of the fire ant also. Comprehensive screening of mono-, di-, and tri-saccharides demonstrated that glucose, fructose, fucose, sucrose, maltose, turanose and raffinose were significant phagostimulants for fire ant workers. It was also found that while D-glucose and L-fucose, the naturally occurring isomers, were active, the opposite diastereomers were not. Any structural modification of the glucose molecule resulted in loss of activity. None of the sugar alcohols evaluated were active. The fire ant is an agricultural pest and the many …

Cc363 1995 Agricultural Outlook And Policy Issues, Lynn Lutgen

University of Nebraska-Lincoln Extension: Historical Materials

Campaign Circular363: 1995 Agricultural Outlook and Policy Issues. Factors Affecting the Livestock Industry. Agricultural Inputs. Commodity Marketing. Land and Taxes Issues. Government Program and Implications.

G95-1261 Five Strategies For Extending Machinery Life, Robert D. Grisso, Steven R. Melvin

University of Nebraska-Lincoln Extension: Historical Materials

Machinery ownership and operation is a major crop and livestock production cost. Several items combined can significantly affect costs, improve machine reliability and improve profit margins.

How to Get Maximum Machinery Life

This fact sheet discusses five strategies to achieve maximum farm machinery life. These strategies are: machinery maintenance, oil analysis, machinery storage, engine tune-ups, and avoiding modification of tractor engines.

G95-1135 Estimating Percent Residue Cover Using The Calculation Method, David P. Shelton, John A. Smith, Paul J. Jasa, Roger Kanable

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide describes how to use the calculation method to estimate the percent of the soil surface that will be covered with crop residue after using residue-disturbing implements and operations. Leaving crop residue on the soil surface is the easiest and most cost-effective method of reducing soil erosion. Research in Nebraska and other midwestern states found that leaving as little as 20 percent of the soil surface covered with crop residue can reduce soil erosion caused by rainfall and flowing water by one-half compared to residue-free conditions. Greater amounts of residue cover will further reduce erosion.

The Effects Of Orientation And Storage Time On The Prediction Of Beef Rib Composition Using Electromagnetic Scanning, B. L. Gwartney, N. L. Meseck, Chris R. Calkins

Department of Animal Science: Faculty Publications

To investigate the effects of orientation of beef ribs in an electromagnetic field and storage time on the prediction of rib composition, 64 ribs (IMPS 103) were scanned (2.5 MHz) in three different orientations: posterior end first (POS), dorsal end first (DOR), and fat side first, blade end down (BLD). Scans were obtained after 1 and 5 d of storage. Scan peak, rib weight, 3/4 fat depth, length, and temperature were used to predict percentage and weight of dissectable and fat-free lean. The mean peak for the BLD scans was four to six times higher than the POS or DOR …

Increasing Concentrations Of 17Β-Estradiol Has Differential Effects On Secretion Of Luteinizing Hormone And Follicle-Stimulating Hormone And Amounts Of Mrna For Gonadotropin Subunits During The Follicular Phase Of The Bovine Estrous Cycle, Andrea S. Cupp, F. N. Kojima, M. S. Roberson, T. T. Stumpf, M. F. Wolfe, L. A. Werth, Roger J. Kittok, H. E. Grotjan, J. E. Kinder

Department of Animal Science: Faculty Publications

The hypothesis tested was that 17β-estradiol (E₂) would increase amounts of mRNA for α, LHβ, and FSHβ subunits during the follicular phase of the estrous cycle prior to the preovulatory surge of gonadotropins in cows. On Day 16 (Day 0 = estrus) of the estrous cycle, all cows were treated with prostaglandin F_2α (PGF_2α). Cows served as intact controls (CONT, n = 4) were ovariectomized (OVX, n = 5), or were ovariectomized and administered E₂ (OVXE, n = 6) in increasing doses starting at the time of treatment with PGF_2α. Cows were …

Dosage Of The Synthetic Progestin, Norgestomet, Influences Luteinizing Hormone Pulse Frequency And Endogenous Secretion Of 17Β-Estradiol In Heifers, T. Sanchez, M. E. Wehrman, F. N. Kojima, Andrea S. Cupp, E. G. M. Bergfeld, K. E. Peters, V. Mariscal, Roger J. Kittok, J. E. Kinder

Department of Animal Science: Faculty Publications

The objective of this study was to determine whether there were doses at which the synthetic progestin, norgestomet, could mimic midluteal phase concentrations of progesterone in regulating the secretion of LH and 17β-estradiol in bovine females. Heifers were randomly assigned to one of five groups to receive: 1) one (Norg, n = 5), 2) two (2Norg, n = 5), 3) four (4Norg, n = 5), or 4) eight (8Norg, n = 5) norgestomet implants or to serve as untreated control heifers (control, n = 5). On Day 7 (Day 0 = behavioral estrus), implants containing norgestomet were inserted, and they …

Differences In Pup Birth Weight, Pup Variability Within Litters, And Dam Weight Of Mice Selected For Alternative Criteria To Increase Litter Size, M. A. J/ Van Engelen, Merlyn K. Nielsen, E. L. De A. Ribeiro

Department of Animal Science: Faculty Publications

Selection for litter size had been practiced for 21 generations and relaxed selection for 13 generations in mice. Three replicates were used with four selection criteria: index of components (ovulation rate and ova success), uterine capacity, litter size, and an unselected control. Especially with selection for litter size and the index relative to the control, number of pups born had increased, and differences also occurred in mating weight. Dams of the three replicates and their litters were used to evaluate the effects of accumulated selection on pup birth weight, variability in weight of littermates, and dam's weight at mating and …

Paleoecological Implications Of Plant/Animal Grazing Relations On The Mammoth Steppe Of Eastern Beringia, Andre Antinori

Nebraska Anthropologist

This paper reassesses the areal extent of Mammoth Steppe in Eastern Beringia and discusses some of the ways in which grazing and topography entered into the maintenance of the Mammoth Steppe. Large, generalist grazing mammals, by their grazing style, helped maintain the Mammoth Steppe by removing old-growth and by stimulating grass plants to reproduce vegetatively. These grazers also promoted uniformity in growth-form and uniformity in plant biomass (phytomass) above and below the ground surface. During the late Pleistocene these large, generalist grazers were eliminated from interior Alaska by Paleolndian hunters and their predatory animal companions. The loss of these large, …

Local Action Of Trophoblast Interferons In Suppression Of The Development Of Oxytocin And Oestradiol Receptors In Ovine Endometrium, G. E. Lamming, D. C. Wathes, A. P. F. Flint, J. H. Payne, K. R. Stevenson, J. L. Vallet

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Luteolysis in sheep is associated with uterine secretion of pulses of prostaglandin F_2α(PGF_2α) due to the action of luteal oxytocin on endometrial oxytocin receptors. For pregnancy to become established inhibition of oxytocin receptors is important as an antiluteolytic mechanism. The maternal recognition of pregnancy in cattle and sheep involves production, by the trophoblast, of a type 1 interferon (IFN-τ)that suppresses uterine development of oxytocin receptors and the generation of luteolytic episodes of PGF_2α. The action of IFN-τ in surgically prepared unilaterally pregnant ewes was investigated. Finn-Dorset ewes were anaesthetized on day 6 or 7 …

Probit Analysis Of Correlated Data: Multiple Observations Over Time At One Concentration, James E. Throne, David K. Weaver, Victor Chew, James E. Baker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We present a method to analyze serial time-mortality data from bioassay experiments where successive observations are made on the same group of organisms exposed to 1 concentration of a stimulus (for example, a pesticide). Standard probit techniques are not applicable because such data are correlated. Methods are presented for calculating statistics for the time-mortality line by regressing complementary log-log, logit, or probit transformations of proportion of responders on untransformed time or logarithmic transformations of time; and for calculating confidence limits on lethal time values corresponding to given mortality levels. As a result we have developed a computer program to facilitate …

An Automated Plot Harvest System For Use With A Commercial Forage Harvester, Jeffrey F. Pedersen, Kenneth J. Moore

Department of Agronomy and Horticulture: Faculty Publications

This automated harvest system is based on minimal modification of currently available commercial forage harvesters. Using 6.8 m plots separated by 0.75 m alleys, a single operator can harvest, finely chop, and collect wet plot weights of forage sorghum [Sorghum bicolor (L.) Moench] or pearl millet [Pennisetum glaucum (L.) R. Hr.] in <1 min per plot. Harvested material is deposited in a pile in the center of each plot. Subsamples for moisture and quality analysis can easily and safely be obtained from the pile.

Two New National Space Laws: Russia And South Africa, Frans G. Von Der Dunk

Space, Cyber, and Telecommunications Law Program: Faculty Publications

Increasing private participation in space activities is one of the most farreaching developments relevant for international space law today. The most comprehensive consequence of such private participation in a legal sense is in fact the necessity to establish a national space legislation of some kind, in view of the international obligations arising under responsibility and liability for the state relative to the consequences of these private activities. The rather recent addition in 1993 of two states, the Russian Federation and the Republic of South Africa, to the previously existing number of three states (the United States, Sweden and the United …

Nebraska Earth Systems Education Network Newsletter – Winter 1995

Nebraska Earth Systems Education Network

Content:

Geological Society of America Activities

NESEN Summer Workshops 1995

NESEN Membership Directory Update

NESEN at NATS Results

NASA Project Status

Bonus Activity

Nutrient Changes In Saginaw Bay, Lake Huron, After The Establishment Of The Zebra Mussel (Dreissena Polymorpha), Thomas H. Johengen, Thomas F. Nalepa, Gary L. Fahnenstiel, Greg Goudy

United States Department of Commerce: Staff Publications

Concentrations of particulate and dissolved nutrients in Saginaw Bay, Lake Huron, were examined relative to zebra mussel colonization which occurred summer 1991. The magnitude and spatial pattern of changes indicate that mussels had a significant impact on nutrients in Saginaw Bay. Annual means for total suspended solids, particulate organic carbon, particulate phosphorus, and particulate silica in the inner bay were significantly lower in 1992 and 1993 (post-zebra mussel) than in 1991 (pre-zebra mussel). Annual means decreased from 11.5 mg L^-1, 1.45 mg C L^-1 (121 µM), 20.4 µg P L^-1(0.66 µM), and 1.52 mg SiO …