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Larval Fish Abundance In The Benthic And Surface Drift Of The Missouri River, Ryan L. Ruskamp

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

Knowledge of the larval fish community of the Missouri River is one of the biggest gaps in fisheries research. The Nebraska Game and Parks Commission has sampled the drift of the Missouri River for many years (1983-2015), but these data have not been compiled into a unified assessment. Therefore, the objectives of this study were to: 1) quantify temporal and spatial aspects of larval fish community composition (richness) and structure (abundance) of the surface drift, 2) quantify associations of larval fish communities of the drift to different discharges of the Missouri River, 3) quantify the larval benthic drift community, and …

Integrated Honey Bee Education And Research Aids For Promoting Pollinator Conservation, Courtney Brummel

Department of Entomology: Dissertations, Theses, and Student Research

Extension and outreach programs combine University instruction and research, with off-campus outreach and service to the community. Successful public education requires training in which colleges and their education services provide programs relevant to today’s needs. To better engage with the public, institutions and Extension professionals often partner with private and not-for-profit organizations to provide training opportunities. The following thesis reviews one such partnership with the University of Nebraska-Lincoln and Kimmel Orchard & Vineyard that provides science-focused, on-farm experiences and agriculture production training programming. Chapter 1 reviews their partnership since it began in 2005 and highlights examples of current farm-to-table education …

Soybean Gall Midge (Resseliella Maxima Gagné): Insecticide Efficacy And Seasonal Larval Abundance, Vilma Maria Montenegro Castro

Department of Entomology: Dissertations, Theses, and Student Research

Soybean gall midge, Resseliella maxima Gagné was recently identified as a new species injuring soybean in Nebraska, Iowa, South Dakota, Minnesota, and Missouri. Resseliella maxima injury is caused by its three instars that feed on tissues within the soybean stem. Infested plants exhibit a darkening of the stem, disrupting nutrient flow within the plant and can lead to wilting or death of plants. Total yield losses can occur in the first 30 m of the field in heavily infested sites. The biology and behavior of this newly described insects is not well understood and management strategies for the management of …

Developing Regional Extension Programs And Research Tools For Beekeepers, Sheldon Brummel

Department of Entomology: Dissertations, Theses, and Student Research

The body of work presented in this thesis describes the development and structure of the Regional Great Plains Master Beekeeping training program, which started in 2019, serves 1500+ beekeepers across five Midwest states (IA, KS, MO, NE, WY), and is supported through local partnerships and beekeepers. This chapter also discusses the leadership structure and aspects I developed for program advancement and long-term sustainability, such as incorporating mentorship and volunteer service goals as well as requiring independent projects to become a certified Master beekeeper. Independent projects may focus on research or teaching goals but must illustrate the candidate’s ability to apply …

A Mixed Methods Case Study: Effects Of Instructors’ Beliefs On Incorporation Of Sustainability Curriculum At A Midwestern University, Anna Oetting

School of Natural Resources: Dissertations, Theses, and Student Research

The degree to which sustainability is taught is often varied and inconsistent across colleges, departments, and higher education institutions. However, educating students and future generations regarding the different pillars of sustainability, including economic, social, and environmental topics, is increasing in importance and urgency. A mixed methods case study utilized surveys and interviews to investigate why instructors incorporate sustainability, what impacts course incorporation of sustainability, and barriers that instructors face. This study found that instructors’ beliefs regarding the importance of sustainability relate to the extent to which sustainability is incorporated into their curriculum. Topics of sustainability incorporated into a class are …

Sex Differences In Lateral Hypothalamic Extracellular Glucose Concentrations During Cumulative Dosing Of Methamphetamine In Rats, Joshua A. Jolton

Honors Program: Senior Projects (Public)

The present study experimentally investigated sex differences in lateral hypothalamic glucose concentrations following cumulative dosing of methamphetamine. Male (n=17) and female (n=11) rats were surgically implanted with enzyme-based glucose biosensors in the lateral hypothalamus. Rats then received a saline injection followed by four methamphetamine doses (0.025 mg/kg, 0.05 mg/kg, 0.1 mg/kg, and 0.2 mg/kg) spaced 55 minutes apart. We found that while there was a general decrease in glucose baseline throughout the study for both sexes, the magnitude of the decrease was significantly greater in females compared to males. On a rapid timescale, fixed sex effects existed at the lower …

Genomic Analysis Of Metabolic Differences Found In Clostridium Perfringens That Cause Necrotic Enteritis In Poultry, Connor M. Aylor

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Clostridium perfringens is a common member of gut microbiota in healthy animals, but can also be an important pathogen in human and veterinary medicine. It produces several protein toxins that contribute to both histotoxic and enteric diseases in animals. Necrotic enteritis in poultry has been associated with the NetB toxin of C. perfringens; however, this toxin alone is insufficient to cause disease in infected chickens. While considerable research has focused on the presence of toxins and virulence factors, little has been done to assess the function of metabolic factors on the ability of the bacteria to cause disease. …

Evaluating Traits Influencing Hybrid Wheat Seed Production Using A Double Haploid Population Derived From Freeman × Camelot, Emre Karahan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hybrid wheat (Triticum aestivum L.) cultivars are preferred for their advanced agronomic traits such as a yield improvement increase up to 20%. However, hybrid cultivars remain difficult to produce on a commercial scale due to inadequate pollen dispersal, the closed nature of the wheat floret and the high cost of inducing sterility or restoring fertility in cleistogamous wheat. Redesigning parent-specific traits and understanding their potential interactions with related agronomic traits are needed to breed improved male lines in hybrid seed production fields. To better understand the genetic control of anther extrusion (AE), 174 double haploid lines derived from Freeman …

Cattle Diet Selection During The Growing Season On Upland Sandhills Rangelands, Alejandro Orozco-Lopez

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The Sandhills is a diverse and complex ecosystem comprised of different topographic positions (i.e. slopes and interdunes) that influence grazing distribution, plant species composition, and cattle diet selection. The first objective of this study was to evaluate how grazing intensity influences species composition on slopes and interdunes on native rangeland within the Hillside pasture (160 ha) at the UNL Gudmundsen Sandhill’s Laboratory (GSL). The second objective of this study was to evaluate forage quality of individual species from three plant functional groups (i.e. warm-season grasses, cool-season grasses, and forbs/shrubs) on native rangeland within the Hillside pasture (160 ha) at GSL. …

Fine Mapping And Cloning Of The Major Seed Protein Quantitative Trait Loci On Soybean Chromosome 20, Christina E. Fliege, Russell A. Ward, Pamela Vogel, Hanh Nguyen, Truyen Quach, Ming Guo, João Paulo Gomes Viana, Lucas Borges Dos Santos, James Specht, Thomas Clemente, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed protein content, alleles of the cqSeed protein-003 QTL on chromosome 20 exert the greatest additive effect. The high-protein allele exists in both cultivated and wild soybean (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this QTL to enable positional-based cloning of its underlying causative gene(s). Fine mapping was achieved by developing and testing a series of populations in which the chromosomal region surrounding …

Two Mothers, One Grandmother: Intergenerational Ambivalence In Heterosexual Mother‑Lbq Daughter Relationships, Emily Kazyak, Rosalind D. Kichler, Jess Morrow, Eliza Thor

Department of Sociology: Faculty Publications

Using the theoretical framing of structural ambivalence, which points to how competing cultural norms can cause conflict in family relationships, this paper asks: how does the transition to parenthood affect the intergenerational family relationship between LBQ adult women and their heterosexual mothers? Analyzing qualitative data from interviews with three adult child-parent dyads, we discuss how two cultural norms manifest in these relationships: pronatalism, or the privileging of procreation and heteronormativity, or the privileging of heterosexuality. In some ways, the intergenerational family relationship is strengthened as both LGB daughters and their heterosexual mothers express that the grandchild resulted in their becoming …

Economic Consequences Of The Wolf Comeback In The Western United States, Dana Hoag, Stewart W. Breck, Kevin Crooks, Becky Niemiec

United States Department of Agriculture Wildlife Services: Staff Publications

Gray wolves were eradicated from most of the United States in the 1940’s but have made a comeback in parts of their historic range over the last two decades. First reintroduced into the Greater Yellowstone Ecosystem and central Idaho in the mid-1990’s, wolves have subsequently dispersed into at least 7 western states. Coloradoans became the latest state to take interest in bolstering wolf populations, as residents passed a ballot initiative in November 2020 to reintroduce a self-sustaining population of gray wolves by the end of 2023. Conflicts between people in rural areas that might incur costs (such as livestock loss) …

Dismantling Binary Assumptions In Sex Estimation: Uplifting Trans And Gender Diverse Identities In Forensic Anthropology, Audrey Holbeck

Honors Program: Senior Projects (Public)

Forensic anthropology is a study within the field of physical anthropology that seeks to apply osteological expertise to legal and criminal situations. One of a forensic anthropologists’ most important jobs is to build a biological profile, consisting of age, biological sex, stature, and ancestry, in correspondence to an unidentified decedent. As we enter the third decade of the 21st century, instances of violence against trans and gender non-conforming individuals are unfortunately prominent, however, there has also been more awareness shed on trans activism. Trans individuals are at a higher risk of being victims of violent crime, and thus, forensic anthropologists …

Evaluation Of Protective Efficacy Of Viral Vector Based Swine Influenza Vaccine And A Method For B Cell Culture For Monoclonal Antibody Generation, Sushmita Kumari

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Influenza A virus of the swine (IAV-S) is an economically important swine pathogen that has the potential to spread to humans, thus posing an ongoing public health concern due to its zoonotic potential. Hemagglutinin (HA), the most abundant viral envelope protein, is known to be the key protective antigen. Anti-HA antibodies alone, have been shown to prevent IAV infection. In this study we evaluated the feasibility of a recombinant tri segmented Pichinde virus (PICV) as a viral vector to deliver IAV-S hemagglutinin antigen in pigs. Four groups of weaned pigs (T01-04) were immunized twice with PBS, rPICV-GFP as a vector …

Reflection Types And Students’ Viewing Of Feedback In A First-Year Engineering Course Using Standards-Based Grading, Heidi A. Diefes-Dux, Laura M. Cruz Castro

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Feedback is one of the most powerful and essential tools for learning and assessment, particularly when it provides the information necessary to close an existing gap between actual and reference levels of performance. The literature on feedback has primarily focused on addressing strategies for providing effective feedback rather than aspects of students’ readiness to engage with feedback. Purpose/Hypothesis: This study investigated whether reflection, as a routine pedagogical intervention grounded in self-regulated learning theory, promotes the frequency with which students view feedback. Design/Method: A quasi-experimental design was employed to examine the relationship between the use of four different reflection types, …

Essential Oil Yield, Composition, And Bioactivity Of Sagebrush Species In The Bighorn Mountains, Valtcho D. Zheljazkov, Charles L. Cantrell, Ekaterina A. Jeliazkova, Tess Astatkie, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

Sagebrush (Artemisia spp.) are dominant wild plants in large areas of the U.S., Canada and Mexico, and they include several species and subspecies. The aim was to determine if there are significant differences in essential oil (EO) yield, composition, and biological activity of sagebrush within the Bighorn Mountains, U.S. The EO yield in fresh herbage varied from 0.15 to 1.69% for all species, including 0.25–1.69% in A. tridentata var. vaseyana, 0.64–1.44% in A. tridentata var. tridentata, 1% in A. tridentata var. wyomingensis, 0.8–1.2% in A. longifolia, 0.8–1% in A. cana, and 0.16% in A. ludoviciana. There was …

Understanding Continent-Wide Variation In Vulture Ranging Behavior To Assess Feasibility Of Vulture Safe Zones In Africa: Challenges And Possibilities, Adam Kane, Ara Monadjem, H. K.Ortwin Aschenborn, Keith Bildstein, André Botha, Claire Bracebridge, Evan R. Buechley, Ralph Buij, John P. Davies, Maria Diekmann, Colleen T. Downs, Nina Farwig, Toby Galligan, Gregory Kaltenecker, Chris Kelly, Ryno Kemp, Holger Kolberg, Monique L. Mackenzie, John Mendelsohn, Msafiri Mgumba, Ran Nathan, Aaron Nicholas, Darcy Ogada, Morgan Pfeiffer, W. Louis Phipps, Matteuns D. Pretorius, Sascha Rösner, Dana G. Schabo, Gabriel Lita Shatumbu, Orr Spiegel, Lindy J. Thompson, Jan A. Venter, Munir Virani, Kerri Wolter, Corinne J. Kendall

United States Department of Agriculture Wildlife Services: Staff Publications

Protected areas are intended as tools in reducing threats to wildlife and preserving habitat for their long-term population persistence. Studies on ranging behavior provide insight into the utility of protected areas. Vultures are one of the fastest declining groups of birds globally and are popular subjects for telemetry studies, but continent-wide studies are lacking. To address how vultures use space and identify the areas and location of possible vulture safe zones, we assess home range size and their overlap with protected areas by species, age, breeding status, season, and region using a large continent-wide telemetry datasets that includes 163 individuals …

European Starling Use Of Nest Boxes Relative To Human Disturbance, Bradley Blackwell, Bruce N. Buckingham, Morgan B. Pfeiffer

United States Department of Agriculture Wildlife Services: Staff Publications

gars and to engine and flight surfaces of moored aircraft. We questioned whether consistent removal of nest material would negatively affect use of a nest site, measured by a reduction in material accumulation. We conducted our study on a 2,200-ha site in Erie County, Ohio, USA (41° 22’ N, 82° 41’ W), from April 15 through June 2, 2020. We used 120 wooden nest boxes on utility poles, protected by an aluminum predator guard below the box. Our treatments included (1) twice weekly, repeated nest material removal (RMR; n = 40 nest boxes); (2) complete nest removal, but only after …

First Portfolio: Introduction To Psychology (Psych 181), Chelsea A. Witt

UNL Faculty Course Portfolios

This course portfolio analyzed student learning in Introduction to Psychology, Psych 181 at the University of Nebraska- Lincoln. This course is often taken by first year students of various backgrounds and majors; this course is a pre-requisite for many other psychology courses. In general, the course covers basic psychological concepts in the realm of biopsychology, developmental psychology, social and personality psychology, cognitive psychology, and clinical psychology. Student enrollment for this section is capped at 200. This portfolio outlines my course goal, objectives, and assessments of those goals. There is also a reflection throughout regarding how well students feel supported and …

Benchmark Portfolio For Asci 451/851: Livestock On Range And Pasture, Fall 2021, Kacie L. Mccarthy

UNL Faculty Course Portfolios

The aim of this portfolio is twofold: first, the portfolio serves to provide a means for the author to reflect on and improve their approach to teaching an ACE 10 course. The Livestock on Range and Pasture course (ASCI 451/851) serves seniors and graduate students with a major and/or minor in Animal Science, Grazing Livestock Systems, Agronomy and Horticulture, or Range. Since taking over the course, the last two semesters have included students primarily from Animal Science or Agribusiness with a major or minor in Grazing Livestock Systems, respectively. This Peer Review of Teaching project evaluated student feedback from the …

Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Mckenna Barber

Department of Animal Science: Dissertations, Theses, and Student Research

Genome annotation has a direct impact on the success of genomic studies. Transcriptome analyses and chromatin immunoprecipitation and sequencing (ChIP-seq) have been used to functionally annotate genomes. These methods can identify protein-coding genes, non-coding transcripts, and cis-regulatory elements across the genome. The primary objective of the first study was to functionally annotate the equine genome through the assessment of nine tissues: adipose, brain, heart, lamina, liver, lung, skeletal, muscle, testis, and ovary. In the first project, 150 bp, paired-end RNA sequencing (RNA-seq) libraries were generated in stallion tissues and compared to previously generated mare RNA-seq libraries to quantify variation in …

Biomechanical Analysis Of Athletes Sprinting With Varying Degrees Of Resistance, Michaela Ott

Honors Program: Senior Projects (Public)

Utilizing resistance methods for sprinters is a common approach to their training. In this study, six athletes from the University of Nebraska-Lincoln Women’s Track and Field Team ran a series of sprints using a resistance machine to collect data regarding the change in power output, stride length, level of trunk tilt with respect to the ground, and acceleration throughout a distance of ten meters when different amounts of resistant forces were applied to the athlete. It was hypothesized that as resistance increased, power output would increase, stride length would decrease, the runners would become more horizontal resulting in a larger …

Effects Of Socioeconomic Status On Consumer Behavior And Attitudes Towards A Brand’S Image, Abbey Haymond

Honors Program: Senior Projects (Public)

This research seeks to understand more about the effects of socioeconomic background on consumer behavior and its impact on the perception of brand images. Data from people of different SES were compared to understand how they view advertisements intended for different audiences and their corresponding effects. It was conducted by sending out a confidential, voluntary survey to a convenience sample targeting 100 consumers. Consumers were asked to rate statements, on a scale of one to five, for each of the research questions to assess specific aspects of their consumer attitudes and behaviors. The high SES group has a higher average …

Polyaniline/Cobalt Nanoparticle Composite Films, Rifat Mahbub

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Polymer nanocomposites have been gaining attention because of the enhanced properties of the element materials it can offer. These composites have been used in biological sector as well as quantum computing and memory devices. These are also used as different kind of sensors. Polymer composites incorporated with magnetic nanoparticles can modify the magnetic property of the nanoparticles and that has huge potential to be used in different sort of applications.

This work aims to develop a polymeric nanocomposite incorporating a conducting polymer and magnetic nanoparticles. The influence of the conducting polymer on the magnetic property of the nanoparticles was investigated. …

Geographic Variation In Host Selection In The Spider Wasps Entypus Unifasciatus (Say) And Tachypompilus Ferrugineus (Say) (Hymenoptera: Pompilidae), Ii, Frank E. Kurczewski, Joseph W. Stoll, Rick C. West, Kelly C. Kissane, Paige R. Chesshire, Neil S. Cobb

Insecta Mundi

This paper is the sequel to a 20 year-long (2002–2021) study of geographic variation in host selec­tion in the common American spider wasps (Hymenoptera: Pompilidae) Entypus unifasciatus (Say) (Pepsini) and Tachypompilus ferrugineus (Say) (Pompilini) (rusty spider wasp). Geography and host spider family are strongly linked in both species when 3387 host spider locality records from the years 1918–2021 are mapped. Entypus unifasciatus lycosid host records are plentiful from 43–44° N in the United States and southern Ontario to northern Mexico. Tachypompilus ferrugineus lycosid host records are abundant from southern Ontario and New England southward to Mexico east of the Rocky …

First Record Of Agrilus Cliftoni Knull And Anthaxia Viridifrons Gory (Coleoptera: Buprestidae) In Wisconsin, Lawrence Barringer

Insecta Mundi

Two species of Coleoptera: Buprestidae are reported from Wisconsin for the first time: Agrilus cliftoni Knull and Anthaxia viridifrons Gory. Trapping, distribution information, and additional records are also discussed.

Jewel beetles such as emerald ash borer (EAB) (Agrilus planipennis Fairmaire), continue to be of interest as estab­lished pests (USDA APHIS 2021). Survey programs allow for the detection of EAB as it continues to spread across North America while monitoring for other taxa not yet present in the eastern United States such as the goldspot­ted oak borer (Agrilus. auroguttatus Schaeffer) and oak splendor beetle Agrilus biguttatus Fabricius (CERIS …

Megalurothrips Usitatus Bagnall (Thysanoptera: Thripidae), First Record Of An Important New Pest In Honduras, Jesus Orozco

Insecta Mundi

Megalurothrips usitatus Bagnall (Thysanoptera: Thripidae), an invasive and highly destructive pest of beans, is recorded for the first time for Honduras. The species is illustrated to aid in its identification. Specimens were collected from Phaseolus vulgaris L. (common bean), Crotalaria juncea L. (sunn hemp), and Melampodium divaricatum (Rich.) DC (butter daisy).

Common beans (Phaseolus vulgaris L., (Fabaceae)), are a staple food source in Honduran cuisine, as they are eaten at least once daily by the majority of the population. This crop is a large part of the economy of the country and the only source of income for many …

A Segmented And Clawed Male Foreleg In A Newly Described Genus And Species Of Eumaeine Butterfly (Lepidoptera: Lycaenidae), Robert K. Robbins, Christophe Faynel, Jason P.W. Hall, Robert C. Busby

Insecta Mundi

Grishinata Robbins and Busby, new genus (Lepidoptera: Lycaenidae: Eumaeini), possesses a five-segmented foretarsus with a clawed pretarsus, a trait that differentiates it from all eumaeine genera except Theclopsis Godman and Salvin. Grishinata penny Busby, Hall, and Robbins, new species, differs from all species of Theclopsis (and most Eumaeini) in lacking male secondary sexual organs on the wings or in the abdomen. It is recorded from the eastern slope of the Andes in Ecuador and Peru. We cannot place Grishinata penny in an existing Eumaeini genus based upon its wing pattern, male foreleg structure, lack of male second­ary sexual organs, and …

Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Zygophyllum qatarensis Hadidi across desert populations. The results showed that rhizospheric and endosphere microbiome greatly varied due to soil texture (sandy and gravel). No specific bacterial amplicon sequence variants were observed in the core-microbiome of bulk soil and rhizosphere, however, bacterial genus Alcaligenes and fungal genus Acidea were abundantly distributed across root and shoot endospheres. We also analyzed major nutrients …

Impact Of The Timing And Use Of An Insecticide On Arthropods In Cover-Crop-Corn Systems, Gabriela Inveninato Carmona, Emily Robinson, Julia Nogueira Duarte Campos, Anthony Justin Mcmechan

Department of Entomology: Faculty Publications

Cover crops provide a habitat for pests and beneficial arthropods. Unexpected pest pressure in a cover-crop-to-corn system can occur and result in increased use of insecticides. Eight site-years of on-farm field studies were conducted in 2019, 2020, and 2021. The objective of the study was to evaluate the impact of insecticide timing relative to cover-crop termination on arthropod activity in a cover-crop-to-corn system. The treatments consisted of (i) glyphosate to terminate the cover crop, (ii) glyphosate and pyrethroid tank mix to terminate the cover crop, and (iii) glyphosate to terminate the cover crop and pyrethroid application 25 days after the …