Digital Commons Network^™

Two Fingerprinting Sets For Humulus Lupulus Based On Kasp And Microsatellite Markers, Mandie Driskill, Katie Pardee, Kim E. Hummer, Jason D. Zurni, Keenan L. Amundsen, Annette Wiles, Claudia Wiedowi, Josef Patza, John A. Henningi, Nahla V. Bassili

Department of Agronomy and Horticulture: Faculty Publications

Verification of clonal identity of hop (Humulus lupulus L.) cultivars within breeding programs and germplasm collections is vital to conserving genetic resources. Accurate and economic DNA-based tools are needed in dioecious hop to confirm identity and parentage, neither of which can be reliably determined from morphological observations. In this study, we developed two fingerprinting sets for hop: a 9-SSR fingerprinting set containing high-core repeats that can be run in a single PCR reaction and a kompetitive allele specific PCR (KASP) assay of 25 single nucleotide polymorphisms (SNPs). The SSR set contains a sex-linked primer pair, HI-AGA7, that was used …

First Reproductive Evidence For The Slender Glass Lizard (Ophisaurus Attenuatus) In Nebraska, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Slender Glass Lizard (Ophisaurus attenuatus) reproduces across parts of northern and central Kansas, but its distribution extends into southern Nebraska. In Nebraska, not a single aspect of reproductive activity has been reported for this species in the state. Herein, we report on the first documentation of reproduction in Nebraska. On 22 September 2021, we captured two hatchling Slender Glass Lizards at the Ash Grove Wildlife Management Area in Franklin County, Nebraska. We calculated that those individuals likely hatched around the first week of September. Presence and reproduction of Slender Glass Lizards at the study site likely was …

Two Magnaporthe Appressoria-Specific (Mas) Proteins, Momas3 And Momas5, Are Required For Suppressing Host Innate Immunity And Promoting Biotrophic Growth In Rice Cells, Ziwen Gong, Na Ning, Zhiqiang Li, Xin Xie, Richard Wilson, Wende Liu

Department of Plant Pathology: Faculty Publications

In the devastating rice blast fungus Magnaporthe oryzae, six Magnaporthe appressoria-specific (MAS) proteins are encoded by MoGAS1, MoGAS2 and MoMAS3–MoMAS6. MoGAS1 and MoGAS2 were previously characterized as M. oryzae virulence factors; however, the roles of the other four genes are unknown. Here, we found that, although the loss of any MAS gene did not affect appressorial formation or vegetative growth, ΔMomas3 and ΔMomas5 mutant strains (but not the others) were reduced in virulence on susceptible CO-39 rice seedlings. Focusing on ΔMomas3 and ΔMomas5 mutant strains, we found that they could penetrate …

Can We Use Antipredator Behavior Theory To Predict Wildlife Responses To High-Speed Vehicles?, Ryan B. Lunn, Bradley Blackwell, Travis L. Devault, Esteban Fernández-Juricic

United States Department of Agriculture Wildlife Services: Staff Publications

Animals seem to rely on antipredator behavior to avoid vehicle collisions. There is an extensive body of antipredator behavior theory that have been used to predict the distance/time animals should escape from predators. These models have also been used to guide empirical research on escape behavior from vehicles. However, little is known as to whether antipredator behavior models are appropriate to apply to an approaching high-speed vehicle scenario. We addressed this gap by (a) providing an overview of the main hypotheses and predictions of different antipredator behavior models via a literature review, (b) exploring whether these models can generate quantitative …

Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao

Center for Plant Science Innovation: Faculty Publications

Plasmodiophora brassicae is a soil-borne pathogen that attacks the roots of cruciferous plants and causes clubroot disease. CircRNAs are noncoding RNAs, widely existing in plant and animal species. Although knowledge of circRNAs has been updated continuously and rapidly, information about circRNAs in the regulation of clubroot disease resistance is extremely limited in Brassica rapa. Here, Chinese cabbage (BJN 222) containing clubroot resistance genes (CRa) against P. brassicae Pb4 was susceptible to PbE. To investigate the mechanism of cicRNAs responsible for clubroot disease resistance in B. rapa, circRNA-seq was performed with roots of ‘BJN 222’ at 0, 8, and 23 days …

Undergraduate Students’ Accuracy & Confidence In Detecting Errors In Biological Models Related To Gpa, Mckenna Elliott, Joseph Dauer, Carrie Clark, Mei Grace Behrendt

UNL Student Research Days Posters, Undergraduate

Research Questions 1. Does students’ abilities to Accurately detect errors relate to their GPA? 2. Which concepts affect student ability to Accurately and Confidently detect errors?

GPA was positively related to accuracy but was unrelated to confidence (Fig. 1)

Subject areas affected students’ accuracy and confidence § Students were more accurate on ecology & evolution models (Fig. 2) § Students were more confident in ecology models and less confident in physiology models (Fig. 2)

Variation in student ability and subject area competency can provide teachers with places to focus and improve science understanding.

Similar to the work of Clark et …

Terrestrial Carbon Isotope Stratigraphy And Mammal Turnover During Post-Petm Hyperthermals In The Bighorn Basin, Wyoming, Usa, Sarah J. Widlansky, Ross Secord, Kathryn E. Snell, Amy E. Chew, William C. Clyde

Department of Earth and Atmospheric Sciences: Faculty Publications

Paleogene hyperthermals, including the Paleocene–Eocene Thermal Maximum (PETM) and several other smaller events, represent global perturbations to Earth’s climate system and are characterized by warmer temperatures, changes in floral and faunal communities, and hydrologic changes. These events are identified in the geologic record globally by negative carbon isotope excursions (CIEs), resulting from the input of isotopically light carbon into Earth’s atmosphere. Much about the causes and effects of hyperthermals remains uncertain, including whether all hyperthermals were caused by the same underlying processes, how biotic effects scale with the magnitude of hyperthermals, and why CIEs are larger in paleosol carbonates relative …

Influence Of Acute Water Ingestion And Prolonged Standing On Raw Bioimpedance And Subsequent Body Fluid And Composition Estimates, Grant M. Tinsley, Matthew T. Stratton, Patrick S. Harty, Abegale D. Williams, Sarah J. White, Christian Rodriguez, Jacob R. Dellinger, Baylor A. Johnson, Robert W. Smith, Eric T. Trexler

Department of Nutrition and Health Sciences: Faculty Publications

This study evaluated the influence of acute water ingestion and maintaining an upright posture on raw bioimpedance and subsequent estimates of body fluids and composition. Twenty healthy adults participated in a randomized crossover study. In both conditions, an overnight food and fluid fast was followed by an initial multi-frequency bioimpedance assessment (InBody 770). Participants then ingested 11 mL/kg of water (water condition) or did not (control condition) during a 5-minute period. Thereafter, bioimpedance assessments were performed every 10 minutes for one hour with participants remaining upright throughout. Linear mixed effects models were used to examine the influence of condition and …

Quantifying Predator Functional Responses Under Field Conditions Reveals Interactive Effects Of Temperature And Interference With Sex And Stage, Kyle E. Coblentz, Amber Squires, Stella Uiterwaal, John Delong

School of Biological Sciences: Faculty Publications

1. Predator functional responses describe predator feeding rates and are central to predator–prey theory. Originally defined as the relationship between predator feeding rates and prey densities, it is now well known that functional responses are shaped by a multitude of factors. However, much of our knowledge about how these factors influence functional responses is based on laboratory studies that are generally logistically constrained to examining only a few factors simultaneously and that have unclear links to the conditions organisms experience in the field.
2. We apply an observational approach for measuring functional responses to understand how sex/stage differences, temperature and predator densities …

Ignoring Species Availability Biases Occupancy Estimates In Single-Scale Occupancy Models, Graziella V. Direnzo, David A. W. Miller, Evan H. C. Grant

United States Geological Survey: Staff Publications

1. Most applications of single-scale occupancy models do not differentiate between availability and detectability, even though species availability is rarely equal to one. Species availability can be estimated using multi-scale occupancy models; however, for the practical application of multi-scale occupancy models, it can be unclear what a robust sampling design looks like and what the statistical properties of the multi-scale and single-scale occupancy models are when availability is less than one.
2. Using simulations, we explore the following common questions asked by ecologists during the design phase of a field study: (Q1) what is a robust sampling design for the multi-scale occupancy …

Maize Leaf Appearance Rates: A Synthesis From The United States Corn Belt, Caio L. Dos Santos, Lori J. Abendroth, Jeffrey A. Coulter, Emerson D. Nafziger, Andrew E. Suyker, Jianming Yu, Patrick S. Schnable, Sotirios V. Archontoulis

School of Natural Resources: Faculty Publications

The relationship between collared leaf number and growing degree days (GDD) is crucial for predicting maize phenology. Biophysical crop models convert GDD accumulation to leaf numbers by using a constant parameter termed phyllochron (°C-day leaf⁻¹) or leaf appearance rate (LAR; leaf ^oC-day⁻¹). However, such important parameter values are rarely estimated for modern maize hybrids. To fill this gap, we sourced and analyzed experimental datasets from the United States Corn Belt with the objective to (i) determine phyllochron values for two types of models: linear (1-parameter) and bilinear (3-parameters; phase I and II phyllochron, and transition …

Als-Associated Kif5a Mutations Abolish Autoinhibition Resulting In A Toxic Gain Of Function, Desiree M. Barron, Adam R. Fenton, Sara Saez-Atienzar, Anthony Giampetruzzi, Aparna Sreeram, Shankaracharya, Pamela J. Keagle, Victoria R. Doocy, Nathan J. Smith, Eric W. Danielson, Megan Andresano, Mary C. Mccormack, Jaqueline Garcia, Valérie Bercier, Ludo Van Den Bosch, Jonathan R. Brent, Claudia Fallini, Brian J. Traynor, Erica L.F. Holzbaur, John E. Landers

Biochemistry -- Faculty Publications

Understandingthepathogenicmechanismsof diseasemutations is critical toadvancingtreatments.ALS-associated mutations in the gene encoding the microtubulemotor KIF5A result in skipping of exon 27 (KIF5ADExon27) and the encoding of a protein with a novel 39 amino acid residue C-terminal sequence. Here, we report that expression of ALS-linked mutant KIF5A results in dysregulated motor activity, cellular mislocalization, altered axonal transport, and decreased neuronal survival. Single-molecule analysis revealed that the altered C terminus of mutant KIF5A results in a constitutively active state. Furthermore,mutant KIF5A possesses altered protein and RNA interactions and its expression results in altered gene expression/splicing. Taken together, our data support the hypothesis that causative …

Variation In Bacterial Community Structure Under Long-Term Fertilization, Tillage, And Cover Cropping In Continuous Cotton Production, Ning Duan, Lidong Li, Xiaolong Liang, Aubrey Fine, Jie Zhuang, Mark Radosevich, Sean M. Schaeffer

Department of Agronomy and Horticulture: Faculty Publications

Agricultural practices alter the structure and functions of soil microbial community. However, few studies have documented the alterations of bacterial communities in soils under long-term conservation management practices for continuous crop production. In this study, we evaluated soil bacterial diversity using 16S rRNA gene sequencing and soil physical and chemical properties within 12 combinations of inorganic N fertilization, cover cropping, and tillage throughout a cotton production cycle. Soil was collected from field plots of the West Tennessee Agriculture Research and Education Center in Jackson, TN, United States. The site has been under continuous cotton production for 38 years. A total …

Optimization Of Sample Unit Size For Sampling Stink Bugs (Hemiptera: Pentatomidae) In Soybean, Arthur V. Ribeiro, Rafael Carlesso Aita, Daniela T. Pezzini, Christina D. Difonzo, Thomas Hunt, Janrt J. Knodel, Christian H. Krupke, Lia Marchi-Werle, Andrew P. Michel, Nicholas J. Seiter, Robert J. Wright, William D. Hutchison, Robert L. Koch

Department of Entomology: Faculty Publications

Cost-effective and reliable sampling procedures are crucial for integrated pest management. Sweep net sampling is commonly used for stink bugs (Hemiptera: Pentatomidae) in soybean, with sample size being the number of sets of sweeps, and sample unit size the number of sweeps in each set. Sample unit size has received little attention, but can affect sampling parameters. Here, two sample unit sizes (10 vs. 25 sweeps) were compared for the sampling of stink bug taxa. On average, sampling for stink bugs took 3.6 more minutes with the 25-sweep than with the 10-sweep sample unit size. Generally, estimates of the mean …

Ubjective Information And Survival In A Simulated Biological System, Tyler S. Barker, Massimiliano Pierobon, Peter J. Thomas

School of Computing: Faculty Publications

Information transmission and storage have gained traction as unifying concepts to characterize biological systems and their chances of survival and evolution at multiple scales. Despite the potential for an information-based mathematical framework to offer new insights into life processes and ways to interact with and control them, the main legacy is that of Shannon’s, where a purely syntactic characterization of information scores systems on the basis of their maximum information efficiency. The latter metrics seem not entirely suitable for biological systems, where transmission and storage of different pieces of information (carrying different semantics) can result in different chances of survival. …

Considerations For Establishing Silvopastures On Wooded Sites, Brett Chedzoy, Ashley Conway, John Fike, Kate Macfarland

Agroforestry Notes (USDA-NAC)

Like other conservation and production practices, silvopasture management is not the right fit for every site or every land manager. High quality woodlands with high native species diversity are generally not a good starting point for silvopasture establishment. However, silvopasture provides an opportunity for active forest management on other sites that have been neglected or poorly managed with undesirable species composition, invasive species concerns, and other challenges. In some situations, silvopasture management can be used to aid in ecosystem restoration or fuels mitigation. The land manager and others in the silvopasture team should consider natural resource issues highlighted in this …

Wiley Journal Package: Unl Download Activity By Subject, David C. Tyler, Casey Hoeve, David Macaulay, Robin Mcclanahan

University of Nebraska-Lincoln Libraries: White Papers

Because the field of librarianship has been reconsidering its relationship to commercial academic publishers and their 'Big Deal' journal packages, the authors decided to review the University of Nebraska-Lincoln's usage of Wiley's package. In this report, the authors looked into whether UNL's downloads by subject were such that subscribing to a number of hypothetical smaller, subject-specific packages, rather than to the entire Wiley package, might be a viable strategy.

Fish & Wildlife News: Spring 2022, Matt Trott

Fish and Wildlife News

No abstract provided.

Salt In Our Bones, Hannah Demma

School of Art, Art History, and Design: Theses and Student Creative Work

In my studio I lead a rich fantasy life. I am excited and enchanted by the interplay of color, pattern, and texture in a variety of mediums — but always involving paper, most often, paper I’ve handmade. I look to the natural world for inspiration in my work. Approaching the work as a scientist or naturalist might, I observe, hypothesize, and run experiments. Then I interpret and process my findings. I consider creative play and intuitive investigation into materials hallmarks of my practice.

When I watch documentaries about sea life, or read about the discoveries of marine scientists, I feel …

Alternative Pathway To Photorespiration Protects Growth And Productivity At Elevated Temperatures In A Model Crop, Amanda P. Cavanagh, Paul F. South, Carl J. Bernacchi, Donald R. Ort

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adapting crops to warmer growing season temperatures is a major challenge in mitigating the impacts of climate change on crop production. Warming temperatures drive greater evaporative demand and can directly interfere with both reproductive and vegetative physiological processes. Most of the world’s crop species have C3 photosynthetic metabolism for which increasing temperature means higher rates of photorespiration, wherein the enzyme responsible for fixing CO2 fixes O2 instead followed by an energetically costly recycling pathway that spans several cell compartments. In C3 crops like wheat, rice and soybean, photorespiration translates into large yield losses that are predicted to increase as global …

Journal Of The National Collegiate Honors Council, Vol. 23, No. 1, Spring/Summer 2022

Journal of the National Collegiate Honors Council Online Archive

Forum Essays on “The Value of Honors to its Graduates”: Authors: Paul Ewing, University of Toledo; Andy Walker, University of Tennessee at Chattanooga; Laura Barrett, LIU Brooklyn; John Major, Ohio State University; Teri Grieb, Columbia College, South Carolina; James A. Keller, University of Delaware; LLeweLLyn Cooper, University of Alabama at Birmingham; Ayesha Ahmed, Northeastern Illinois University; Mary Beth Messner, Youngstown State University; Eric W. Miller, West Virginia University; Sara McCane-Bowling, Eastern Kentucky University; Michelle Panuccio, Youngstown State University; Lia M. Shore, Georgia Perimeter College, Dunwoody; Jennifer N. Dulin, Texas A&M University; Pepper Hayes, …

A Checklist Of The Hawkmoths (Lepidoptera: Sphingidae) Of The Cayman Islands: With Implications For The Pollination Of The Ghost Orchid Dendrophylax Fawcettii Rolfe (Orchidaceae: Angraecinae) And Consideration Of Bat Predation, M. C. Rose-Smyth, R. R. Askew, P. A. Van B. Stafford, M. J. Simon, G. J. Gross, R. Rozycki

Insecta Mundi

This checklist synthesises historic collections of Sphingidae (Lepidoptera) made during the summer months in the Cayman Islands in 1938 and 1975 with modern records drawn from a variety of sources. We report observations and collections made in all seasons of the year and provide natural history and larval food details. Four species, Phryxus caicus (Cramer) in all three islands, plus Isognathus rimosa (Grote), Enyo lugubris (Linnaeus), and Eumorpha satellitia (Linnaeus) in Grand Cayman only, are here reported as new records, for a total of 25 sphingid species occurring in the Cayman Islands. Seven species are new records for Grand Cayman, …

Caring For Our Communities Of Practice In Educational Development, Christopher V. H.-H. Chen, Katherine Kearns, Lynn Eaton, Darren S. Hoffmann, Denise Leonard, Martin Samuels

To Improve the Academy: A Journal of Educational Development

Given the backdrop of multiple concurring crises—a global pandemic, political instability and violence, and multiple structural inequalities—we see the problem of now as this: How do educational developers continue to address the wicked problems in teaching and learning when we are simply so exhausted? Our article presents the importance of communities of practice for educational developers, inviting us to witness and name the communities in which we belong; the important functions they engage; who they nurture and how; and what care is undertaken to sustain these groups and ourselves. To help educational developers understand and appreciate the ways that communities …

Contexts For Agency: A Framework For Managing Educational Development Work, Kathleen Landy, Anna L. Bostwick Flaming, Suzanne Tapp, Eric C. Kaldor

To Improve the Academy: A Journal of Educational Development

Often working in multiple roles and operating at multiple scales, educational developers deal with layered tensions and a complex context that can be difficult for an individual or team to reconcile. In May 2020, the authors participated in a cross-institutional scholarly project, the Pandemic Educational Development Research Collaborative (PEDRC), designed to explore the impact of multiple crises (e.g., the COVID-19 pandemic and our collective civil and political unrest) and associated large-scale instructional changes on educational developers and their work. The Contexts for Agency framework reflects the project’s emergent theme that the circumstances in which we act have considerable influence on …

Beyond Backward Design, Or, By The End Of This Article, You Should Be Able To Imagine Some Alternatives To Learning Objectives, Michael Mccreary

To Improve the Academy: A Journal of Educational Development

If the challenges of teaching and learning do not amount to simple empirical questions about effective pedagogical strategies but are instead complex “wicked problems” that may be impossible to solve, where does that leave the practice of backward design? Drawing on the intellectual history of instructional design, I argue that the use of learning objectives, in particular, may not meet the comprehensive challenges of educational development today. Rather than rehashing perennial critiques that learning objectives overly instrumentalize the educational process or are not sufficiently student-centered, I ask what is missed by focusing on what students will know and be able …

Taking Teaching And Learning Seriously: Approaching Wicked Consciousness Through Collaboration And Partnership, Adam H. Smith, Laurie L. Grupp, Lindsay Doukopoulos, John C. Foo, Barbara J. Rodriguez, Janel Seeley, Linda M. Boland, Laurel L. Hester

To Improve the Academy: A Journal of Educational Development

The ongoing COVID-19 pandemic has demanded large-scale collaboration within all organizations, including higher education, and taking teaching and learning seriously, in this moment, means leveraging partnerships to address the wicked (large, complex) problems cited by Bass (2020). These problems are not ours alone to solve; rather, we make the case for a “wicked consciousness,” an amalgam of perspectives, in educational development. Guided by intellectual humility, our success as educational developers ought to be measured by the quality of our collaborations as well as our ability to learn with others, form equitable partnerships, and lead others by our example.

Toward Institutionalizing Successful Innovations In The Academy, Sarah B. Wise, Courtney Ngai, Joel Christopher Corbo, Mark A. Gammon, Jaclyn K. Rivard, Clara E. Smith

To Improve the Academy: A Journal of Educational Development

Due to the “wicked problem” of the Academy’s resistance to innovation, new teaching and learning programs struggle to become integrated into the fabric of the Academy, which slows the uptake of evidence-based practices. This wicked problem is rooted in the lack of slow, intentional mechanisms for cultural change in the Academy. In this article, we analyze the institutionalization journey of the Departmental Action Team (DAT) project, which is a model for slow, intentional change. Over the last four years, partnering with two campus centers for teaching and learning (CTLs) allowed the DAT project to make institutionalization progress.

This analysis is …

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Edition 4, April 2022, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

Nebraska Game and Parks Wildlife education resource giveaway

Eye on curriculum and resources

Teacher opportunities

List of new partnerships

Ask an expert

Eye on CASNR research: Amy Schmidt

Send your students to campus

Success stories

Analyzing The Impact Different Teaching Strategies On Student Learning Outcomes For The Online Graduate Class Soil Chemistry & Mineralogy (Agro/Nres/Soil 455/855), Michael Kaiser Phd

UNL Faculty Course Portfolios

In this portfolio teaching materials, activities, and assessments applied in a Soil Chemistry & Mineralogy” online graduate class are analyzed towards their suitability to achieve learning objectives and class goals. For this, data from class surveys, a decision matrix, and individual student feedback are the main sources of information. The survey revealed that the learning objectives are successfully addressed. Lecture videos, quizzes, exams, scientific paper reading assignments, and a review paper assignment were evaluated to be suitable in achieving these objectives. The developed matrix and individual student feedback suggest that the overall student learning satisfaction would benefit from more activities …

Unl Emeriti And Retirees Association Newsletter, Spring 2022

UNL Emeriti and Retirees Association: Newsletters and Other Materials

University of Nebraska-Lincoln Emeriti and Retirees Association Newsletter, Spring 2022