Digital Commons Network^™

Polyacrylamide For Surface Irrigation To Increase Nutrient-Use Efficiency And Protectwater Quality, Rodrick D. Lentz, Robert E. Sojka, Charles W. Robbins, Dennis C. Kincaid, Dale T. Westermann

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Furrow irrigation systems have a greater application capacity, are less costly, and use less energy than sprinkler systems but furrow irrigation produces greater runoff, erosion, and deep percolation losses. Phosphorus (P) and nitrogen (N) losses are associated with runoff sediment, and can be minimized by eliminating irrigationinduced erosion. Excessive leaching of inorganic and organic solutes commonly occurs at the inflow region of furrow irrigated fields where infiltration opportunity times are longer. In one conservation practice, a high molecular weight, anionic polyacrylamide (PAM) is applied to advancing furrow stream flows at a concentration of 10 mg L^-1. Because PAM …

Worms, Nematoda, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Nematodes are the most speciose phylum of metazoa on earth. Not only do they occur in huge numbers as parasites of all known animal groups, but also they are found in the soils, as parasites of plants, and in large numbers in the most extreme environments, from the Antarctic dry valleys to the benthos of the ocean. They are extremely variable in their morphological characteristics, with each group showing morphological adapta­tions to the environment that they inhabit. Soil-dwelling forms are extremely small; many marine species have long and complex setae; and parasitic species man­ifest amazingly great reproductive potential and large …

Phylogenetic Position Of Eimeria Antrozoi, A Bat Coccidium (Apicomplexa: Eimeriidae) And Its Relationship To Morphologically Similar Eimeria Spp. From Bats And Rodents Based On Nuclear 18s And Plastid 23s Rdna Sequences, Xiaomin Zhao, Donald W. Duszynski, Eric S. Loker

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Partial plastid 23S and nuclear 18S rDNA genes were amplified and sequenced from 2 morphologically similar Eimeria species, E. antrozoi from a bat (Antrozous pallidus) and E. arizonensis from deer mice (Peromyscus spp.), as well as some other Eimeria species from bats and rodents. The phylogenetic trees clearly separated E. antrozoi from E. arizonensis. The phylogenies based on plastid 23S rDNA data and combined data of both plastid and nuclear genes grouped 2 bat Eimeria and 3 morphologically similar Eimeria species from rodents into 2 separate clades with high bootstrap support (100%, 3 rodent Eimeria species; …

Phylogenetic Analysis Of The Rhabdocoela (Platyhelminthes) With Emphasis On The Neodermata And Relatives, David Zamparo, Daniel R. Brooks, Deborah A. Mclennan, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic systematic analysis of 24 taxa representing the rhabdocoel platyhelminths, based on a suite of 89 morphological characters, produced two equally parsimonious trees, 181 steps long, with a consistency index (CI) of 0.69 and a rescaled consistency index (RCI) of 0.56, differing only with respect to that portion of the tree containing Umagillidae, Acholadidae, Graffillinae, Pseudograffillinae, Pterastericolidae and Hypoblepharinidae. Our results accommodate all previously proposed sister taxa to the Neodermata in a single clade in which ((Dalyelliidae + Temnocephalida) Typhloplanidae) is the sister group of ((Fecampiidae + Urastoma) (Udonella ((Aspidogastrea + Digenea) (Monogenea (Gyrocotylidea (Amphilinidea + Eucestoda)))))). Bootstrap …

Genomic Regions That Underlie Soybean Seed Isoflavone Content, K. Meksem, V. N. Njiti, W. J. Banz, M. J. Iqbal, My. M. Kassem, David L. Hyten, J. Yuang, T. A. Winters, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Soy products contain isoflavones (genistein, daidzein, and glycitein)that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL)fr om the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds fromeach RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The …

Prevalence Of Eimeria (Apicomplexa: Eimeriidae) In Reintroduced Gunnison's Prairie Dogs (Cynomys Gunnisoni), M. M. Ryan, K. H. Decker, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fecal samples from 54 Gunnison’s prairie dogs (Cynomys gunnisoni) from Albuquerque, NM were analyzed for the presence of coccidia and all were positive. They were then relocated to an abandoned prairie dog town on the Sevilleta Long Term Ecological Research (LTER) site. Six Eimeria species, E. callospermophili, E. cynomysis, E. pseudospermophili (new host record), E. spermophili, E. ludoviciani and E. vilasi (new host record) were found in Albuquerque animals, but only two species, E. callospermophili and E. vilasi were present in relocated hosts. A significant (P < 0.05) reduction was seen in the prevalence of E. vilasi (72% vs. 13%) and in the prevalence of infections …

Phylogey Of Steinernema Travassos, 1927 (Cephalobina: Steinnematidae) Inferred From Ribosomal Dna Sequences And Morphological Characters, S. Patricia Stock, James F. Campbell, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Entomopathogenic nematodes in Steinernema, together with their symbiont bacteria Xenorhabdus, are obligate and lethal parasites of insects that can provide effective biological control of some important lepidopteran, dipteran, and coleopteran pests of commercial crops. Phylogenetic relationships among 21 Steinernema species were estimated using 28S ribosomal DNA (rDNA) sequences and morphological characters. Sequences of the rDNA internal transcribed spacers were obtained to provide additional molecular characters to resolve relationships among Steinernema carpocapsae, Steinernema scapterisci, Steinernema siamkayai, and Steinernema monticolum. Four equally parsimonious trees resulted from combined analysis of 28S sequences and 22 morphological characters. Clades inferred from …

Uneasy Unions: Quality Effects Of Rye Chromatin Transfers To Wheat, Robert A. Graybosch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A number of genes of agronomic importance have been transferred from rye (Seale cereale L.) to its close relative, common bread wheat (Triticum aestivum L). Largely through the production of interspecific chromosomal translocation and substitution lines, rye chromatin now rides within the genome of a large number of wheat breeding lines and cultivars. Rye chromosomal materials have been used to transfer resistance genes to fungal pathogens, especially rusts and powdery mildew, resistance to insect pests, and in some cases, may enhance grain yield, grain yield stability, and grain protein content. Unfortunately, the transfer of some rye chromosomal materials …

Net Grassland Carbon Flux Over A Subambient To Superambient Co2 Gradient, P. C. Mielnick, W. A. Dugas, H. B. Johnson, H. W. Polley, J. Sanabria

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Increasing atmospheric CO₂ concentrations may have a profound effect on the structure and function of plant communities. A previously grazed, central Texas grassland was exposed to a 200-µmol mol^-1 to 550 µmol mol^-1 CO₂ gradient from March to mid-December in 1998 and 1999 using two, 60-m long, polyethylene-covered chambers built directly onto the site. One chamber was operated at subambient CO₂ concentrations (200-360 µmol mol^-1 daytime) and the other was regulated at superambient concentrations (360-550 µmol mol^-1). Continuous CO₂ gradients were maintained in each chamber by photosynthesis during the day and …

Research Needs Concerning Non-Target Impacts Of Biological Control Introductions, K.R. Hopper

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two workshops were held in 1999 on research needs concerning non-target impacts of biological control introductions. One took place on the Internet for 9 months, and the other in Montpellier, France, for 1 day. Altogether, over 200 researchers from over 30 countries participated, representing a wide variety of viewpoints on the issues involved. The deliberations of these workshops are summarized here. Both workshops highlighted the need for retrospective studies to: (i) identify cases of significant non-target impacts; (ii) explore mechanisms involved when such impacts are found; (iii) evaluate the reliability of host-range testing protocols; and (iv) determine whether and under …

Meiotic Stability, Chloroplast Dna Polymorphisms, And Morphological Traits Of Upland X Lowland Switchgrass Reciprocal Hybrids, J. M. Martinez-Reyna, Kenneth P. Vogel, Carol Caha, Donald J. Lee

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass (Panicum virgatum L.) has two cytotypes or cytoplasm types, L and U, that are associated with the lowland and upland ecotypes, respectively. The L cytotypes are tetraploids while the U cytotypes can be either tetraploids or octaploids. The objective of this research was to characterize meiotic stability of reciprocal crosses of U and L plants as indicated by chromosome pairing at meiosis and to determine the mode of inheritance of chloroplast DNA (cpDNA) in the hybrids of these cytotypes. Morphological markers that characterize the parents and hybrids also were investigated to confirm that progeny were true hybrids. Reciprocal …

Soil Property Changes During Conversion From Perennial Vegetation To Annual Cropping, Brian J. Wienhold, Donald L. Tanaka

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Management practices for conversion of land supporting perennial vegetation to crop production are needed. Effect of haying (hayed or not hayed), cropping (annual crop with no-tillage, minimum tillage, or conventional tillage, and no-tilled perennial crop), and N fertilization (0 or 67 kg ha^-1) on soil properties were measured in 1995 and 1997 at a Conservation Reserve Program (CRP) site in North Dakota having an Amor loam (Fine-loamy, mixed, superactive, frigid, Typic Haplustoll) soil in a spring wheat (Triticum aestivum L.), winter wheat, pea (Pisum sativum L.) rotation. Soil physical properties were not affected negatively by the …

Nutrients In Runoff Following The Application Of Swine Manure To Interrill Areas, John E. Gilley, Bahman Eghball, Brian J. Wienhold, Phillip S. Miller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The P content of swine manure can be reduced through the addition of feed supplements or the use of selected corn hybrids. This study was conducted to compare interrill runoff losses of P and N from three soils following the application of swine manure obtained from selected diets. The soils used in this investigation included a Hersh sandy loam, Pierre silty clay, and Sharpsburg silt loam. Simulated rainfall was applied during both initial and wet runs to a soil pan on which swine manure produced from low phytate corn (LPC), phytase added to the diet (PHY), or a traditional corn …

Functional Classification Of Swine Manure Management Systems Based On Effluent And Gas Emission Characteristics, J. A. Zahn, J. L. Hatfield, D. A. Laird, T. T. Hart, Y. S. Do, A. A. Dispirito

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Gaseous emissions from swine (Sus scrofa) manure storage systems represent a concern to air quality due to the potential effects of hydrogen sulfide, ammonia, methane, and volatile organic compounds on environmnental quality and human health. The lack of knowledge concerning functional aspects of swine manure management systems has been a major obstacle in the development and optimization of emission abatement technologies for these point sources. In this study a classification system based on gas emission characteristics and effluent concentrations of total phosphorus (P) and total sulfur (S) was devised and tested on 29 swine manure management systems in …

Oxidative Metabolism In Plant/Bacteria Interactions: Characterization Of The Oxygen Uptake Response Of Bacteria, C. Jacyn Baker, Norton Mock, Kenneth L. Deahl, Bryan Bailey, Daniel P. Roberts

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An increase in oxygen uptake has been previously described in plant cell suspensions treated with bacteria or bacterial elicitors. These studies, regarding oxygen uptake, have all been undertaken from the perspective of the host plant cell reacting to the invading pathogen. In contrast, here we describe and characterize an increase in oxygen uptake by bacterial cells in response to plant suspensions or autoclaved plant cell filtrates. Autoclaved plant cell filtrates stimulated bacterial oxygen uptake by as much as sevenfold within a few minutes after addition. This oxygen uptake response was proportional to both the concentration of the plant cell filtrate …

Oxidative Metabolism In Plant/Bacteria Interactions: Characterization Of A Unique Oxygen Uptake Response Of Potato Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plant suspension cells have been shown to respond to bacteria or microbial elicitors by producing active oxygen as well as increasing oxygen uptake. Here we characterize a unique two stage oxygen uptake response of potato suspension cells to heat-killed bacteria. Stage 1 occurred within minutes after the addition of heat-killed bacteria; the potato suspension cells responded with a rapid increase in oxygen uptake and reached a steady state approximately 50 % greater than the initial basal rate. Stage 2 began 20-30 min after this new steady state was achieved and was characterized by a slow increase in the oxygen uptake …

Induction Of Defense Responses In Tobacco By The Protein Nep1 From Fusarium Oxysporum, James C. Jennings, Patricia C. Apel-Birkhold, Norton M. Mock, C. Jacyn Baker, James D. Anderson, Bryan A. Bailey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fusarium oxysporum produces a 24-kDa protein, Nep1, which induces necrosis and ethylene production in leaves of many dicot plant species. Detached Nicotiana tabacum L. cv. Xanthi leaves respond with concentration-dependent necrosis after infiltration with Nep1 or when Nep1 is taken up by the vascular tissue. This response follows the induction of ethylene biosynthesis and accumulation of ACC synthase and ACC oxidase transcripts. Pretreating the leaves with 100 µl/l ethylene prior to elicitation enhanced Nep1-induced ethylene production. Nep1 (208 nM) causes extensive necrosis of mature tobacco leaf tissue when applied to Xanthi tobacco as a foliar spray (129 ml/m²). …

Conservation And Evaluation Of Natural Enemies In Ipm Systems For Bemisia Tabaci, Steven E. Naranjo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Conservation biological control emphasizes the preservation and enhancement of natural enemies and is the cornerstone of all approaches to biological control. This review critically examines recent and current research efforts in conservation biological control by predators and parasitoids of Bemisia tabaci worldwide. A large number of natural enemy species have been identified from many agricultural systems. Further research has demonstrated that these natural enemies may act along with other mortality agents to inflict high levels of mortality on populations of B. tabaci. Less effort has been placed on determining the factors constraining or potentially enhancing biological control. The widespread …

Managing Crop Residue With Green Manure, Urea, And Tillage In A Rice–Wheat Rotation, Milkha S. Aulakh, T. S. Khera, John W. Doran, Kevin F. Bronson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Most double-crop grain farmers in South Asia remove or burn cropresidue to facilitate seedbed preparation and to avoid possible yield reductions. This results in loss of soil organic matter (SOM) and nutrients. In this study, we determined whether incorporating wheat (Triticum aestivum L.) residue, rice (Oryza sativa L.) residue, and sesbania (Sesbania aculeta L.) green manure with urea fertilizer N in a rice–wheat cropping system can improve grain yields, N use efficiency, and SOM. We incorporated wheat residue (6 Mg ha^-1, C/N = 94), rice residue (6 Mg ha^-1, C/N = 63), or …

Gamma Sigma Delta, Nebraska Chapter Newsletter, Issue #22, February 2001

Gamma Sigma Delta, Nebraska Chapter: Newsletters

PRESIDENT'S MESSAGE - Merlyn Nielsen, President Nebraska Chapter

NOTES FROM THE HISTORIAN

MEMBERSHIP COORDINATOR REPORT

2001 SCHEDULE OF EVENTS

2001 Scholarship Recognition Reception

GAMMA SIGMA DELTA COMMITTEES, 2001

2001 ANNUAL DUES NOTICE FOR NEBRASKA CHAPTER-GAMMA SIGMA DELTA

Gamma Sigma Delta, Nebraska Newsletter, Issue #22 [#23], August 2001

Gamma Sigma Delta, Nebraska Chapter: Newsletters

PRESIDENT'S MESSAGE - Merlyn Nielsen, President, Nebraska Chapter

IN MEMORIAM

MEMBERSHIP COORDINATOR REPORT

SCHOLARSHIP RECOGNITION RECEPTION AWARDS RECIPIENTS

NOMINATIONS FOR MEMBERSHIP TO GAMMA SIGMA DELTA August 2001

Merit Award Nomination

Teaching Award Nomination

Research Award Nomination

Extension Award Nomination

BALLOT FOR GAMMA SIGMA DELTA TREASURER

Annual Review Of The World Pheasant Association 2000/2001, World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Reports and Other Materials

President's message, Keith C. R. Howman

Chairman of Trustees' report, Richard P. Howard

Treasurer's annual report and accounts, Ian F. Hoggarth

Conservation Committee report, Philip J. K. McGowan

Strategic Development Committee report

Chapters and Affiliate reports

Pheasant Specialist Group report, Peter J. Garson

Partridge, Quail and Francolin Specialist Group report, John P. Carroll

Grouse Specialist Group report, Ilse Storch

Megapode Specialist Group report, René W. R. J. Dekker and Darryl N. Jones

Cracid Specialist Group report, Daniel M. Brooks

The new Conservation Breeding and Aviculture Group for WPA in the United Kingdom, Garry Robbins, Mike Cook, and Keith Chalmers-Watson

Frontal …

The Missouri River And Adaptive Management: Protecting Ecological Function And Legal Process, John H. Davidson, Thomas E. Geu

Nebraska Law Review

This article describes and comments on the management alternatives in the Revised Draft Environmental Impact Statement, Master Water Control Manual, Missouri River (RDEIS) as refined by the newly published National Academy of Sciences project and to assess those alternatives in the context of the eco-regulatory political history of the Missouri River and the new science of complex adaptive systems theory. It includes a general history of the regulation and management of the Missouri River, a procedural history and content summary of the RDEIS as well as the project report on adaptive management of the River by the National Academy of …

Nine New Species Of Lacinipolia (Noctuidae) From Arizona, California And Vicinity, Charles L. Selman, Ron Leuschner

The Taxonomic Report of the International Lepidoptera Survey

This article formalizes the taxonomic conclusions of the senior author’s 1975 successful doctorate dissertation. The nine new species defined in that 1975 dissertation are here formally described in compliance with the rules of the International Code of Zoological Nomenclature (ICZN). The authors have essentially abstracted the original 447 page dissertation and designated types. The new Lacinipolia species are: delongi, aileenae, triplehorni, bucketti, baueri, sharonae, martini, fordi, and franclemonti. The purpose of this paper is simply to make these names available to the scientific community and professional researchers by making them ICZN-compliant.

Records For The Utilization Of Prunus As A Larval Foodplant By 71 Species Of Lepidoptera In Northeast California, Laurence L. Crabtree, Ron Leuschner

The Taxonomic Report of the International Lepidoptera Survey

Twenty-six sites in five northeastern California counties (Shasta, Modoc, Plumas, Lassen, Tehama) were surveyed from 1991 to 1999 for the presence of lepidopteran larvae on naturally occurring shrubs of the genus Prunus. To date, a total of seventy-one species of Lepidoptera from seventeen families have been documented to utilize one or more of the area’s three Prunus species (P. emarginata, P. subcordata, and P. virginiana var. demissa).

A Taxonomic Study Of, And Key To, The Lecithoceridae (Lepidoptera) From Guizhou, China, Chunsheng Wu

The Taxonomic Report of the International Lepidoptera Survey

This paper provides a key to twelve species (in ten genera and three subfamilies) of Lecithoceridae from Guizhou Province, China. Among them, three species are unnamed and eight are new Guizhou Province records. The female of Opacoptera ecblasta Wu is known for the first time and its genitalia are illustrated for the first time.

The Biology, Life History, And Taxonomy Of Celastrina Neglectamajor (Lycaenidae: Polyommatinae)., Harry Pavulaan, David M. Wright

The Taxonomic Report of the International Lepidoptera Survey

The biology and life history of Celastrina neglectamajor is described. This lycaenid butterfly was first described from West Virginia, United States by 19th century lepidopterist, William H. Edwards, as a redescription of Lycaena pseudargiolus (Boisduval & Le Conte). Edwards provided evidence of its unique late spring flight, Appalachian affinity, and larval host Cimicifuga racemosa (Ranunculaceae). Edwards and subsequent authors, most notably Samuel Scudder of New England, treated it as an infrasubspecific form of the common eastern Celastrina ladon. In 1908 Tutt applied the form name neglectamajor, describing it from one of Edwards’ 1884 figures. For nearly a century, …

The Transcriptional Responses Of Respiratory Epithelial Cells To Bordetella Pertussis Reveal Host Defensive And Pathogen Counter-Defensive Strategies, Christopher E. Belcher, Jorg Drenkow, Bettina Kehoe, Thomas R. Gingeras, Nancy Mcnamara, Hassan Lemjabbar, Carol Basbaum, David A. Relman

United States Department of Veterans Affairs: Publications

Bordetella pertussis, the causative agent of whooping cough, has many well-studied virulence factors and a characteristic clinical presentation. Despite this information, it is not clear how B. pertussis interaction with host cells leads to disease. In this study, we examined the interaction of B. pertussis with a human bronchial epithelial cell line (BEAS-2B) and measured host transcriptional profiles by using high-density DNA microarrays. The early transcriptional response to this pathogen is dominated by altered expression of cytokines, DNA-binding proteins, and NFKB-regulated genes. This previously unrecognized response to B. pertussis was modified in similar but non-identical fashions by the anti-inflammatory …

Predicting The Local Dynamics Of Epizootic Rabies Among Raccoons In The United States, James E. Childs, Aaron T. Curns, Meghan E. Dey, Leslie A. Real, Lisa Feinstein, Ottar N. Bjørnstad, John W. Krebs

Zoonotics and Wildlife Disease: Publications

Mathematical models have been developed to explore the population dynamics of viral diseases among wildlife. However, assessing the predictions stemming from these models with wildlife databases adequate in size and temporal duration is uncommon. An epizootic of raccoon rabies that began in the mid-Atlantic region of the United States in the late 1970s has developed into one of the largest and most extensive in the history of wildlife rabies. We analyzed the dynamics of local epizootics at the county level by examining a database spanning more than 20 years and including 35,387 rabid raccoons. The size, number, and periodicity of …

Enzymatic Nixtamalization: An Improved Corn Masa Flour Production Process, Deepak Sahai, David S. Jackson

Department of Food Science and Technology: Public Access Records

A major concern during commercial masa or instant masa flour production is the proper disposal of the excess waste water generated from the nixtamalization process. Nixtamalization causes partitioning of corn solids between nixtamal and waste water. It has been estimated that corn solids loss during nixtamalization varies between 5-14% depending on corn type & quality. Nejayote waste is highly alkaline, with high chemical and biological oxygen demands (BOD & COD) and is considered an environmental pollutant. A typical corn nixtamalization facility processing 200 US tons of corn every day, uses over 50 gallons of water per minute and generates nearly …