Digital Commons Network^™

First Things First: Widespread Nutrient Deficiencies Limit Yields In Smallholder Oil Palm Fields, Hendra Sugianto, Juan Pablo Monzon, Iput Pradiko, Fatima A. Tenorio, Ya Li Lim, Christopher R. Donough, Sunawan, Suroso Rahutomo, Fahmuddin Agus, James Cock, Joni Amsar, Rana Farrasati, Ridho Iskandar, Juan I. Rattalino Edreira, Shofia Saleh, Heri Santoso, Antonius P. Tito, Nadib Ulfaria, Maja A. Slingerland, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Indonesia is the most important oil palm producing country. Nearly 40% of planted area is managed by smallholders, with yields well below the potential. Efforts to increase productivity have focused on the source of planting material, with little attention paid to plant nutrition.

OBJECTIVE: To assess the degree to which current productivity in smallholder oil palm fields is limited by nutrients in scenarios with distinct sources of planting material.

METHODS: We collected detailed data on leaf nutrient concentration from 30 fields to derive minimum sampling size needed to diagnose nutrient status. Subsequently, we collected data on …

P3 And Nia-Pro Of Turnip Mosaic Virus Are Independent Elicitors Of Superinfection Exclusion, Haritha Nunna, Feng Qu, Satyanarayana Tatineni

Department of Plant Pathology: Faculty Publications

Superinfection exclusion (SIE) is an antagonistic interaction between identical or closely related viruses in host cells. Previous studies by us and others led to the hypothesis that SIE was elicited by one or more proteins encoded in the genomes of primary viruses. Here, we tested this hypothesis using Turnip mosaic virus (TuMV), a member of the genus Potyvirus of the family Potyviridae, with significant economic consequences. To this end, individual TuMV-encoded proteins were transiently expressed in the cells of Nicotiana benthamiana leaves, followed by challenging them with a modified TuMV expressing the green fluorescent protein (TuMV-GFP). Three days after …

P3 And Nia-Pro Of Turnip Mosaic Virus Are Independent Elicitors Of Superinfection Exclusion, Haritha Nunna, Feng Qu, Satyanarayana Tatineni

Department of Plant Pathology: Faculty Publications

Superinfection exclusion (SIE) is an antagonistic interaction between identical or closely related viruses in host cells. Previous studies by us and others led to the hypothesis that SIE was elicited by one or more proteins encoded in the genomes of primary viruses. Here, we tested this hypothesis using Turnip mosaic virus (TuMV), a member of the genus Potyvirus of the family Potyviridae, with significant economic consequences. To this end, individual TuMV-encoded proteins were transiently expressed in the cells of Nicotiana benthamiana leaves, followed by challenging them with a modified TuMV expressing the green fluorescent protein (TuMV-GFP). Three days after …

Putrescine Detected In Strains Of Staphylococcus Aureus, Javier Seravalli, Frank Portugal

Department of Biochemistry: Faculty Publications

Most forms of life, including the archaea, bacteria, and eukaryotes synthesize the polyamine putrescine. Although putrescine is widely distributed, several Gram-positive bacteria, including Staphylococcus aureus (S. aureus), appear to be the exceptions. We report here that strains of S. aureus can produce the polyamine putrescine, as well as the derivative N-acetyl-putrescine. Three strains of S. aureus from the American Type Culture Collection (ATCC), one strain listed in the National Center for Biotechnology Information (NCBI) database, whose genomic sequence is well defined, and well as eight strains from S. aureus-induced brain abscesses of individual patients from multiple …

Wildlife Ecological Risk Assessment In The 21st Century: Promising Technologies To Assess Toxicological Effects, Barnett A. Rattner, Thomas G. Bean, Val R. Beasley, Philippe Berny, Karen M. Eisenreich, John E. Elliott, Margaret L. Eng, Phyllis C. Fuchsman, Mason D. King, Rafael Mateo, Carolyn B. Meyer, Jason M. O'Brien, Christopher J. Salice

United States Geological Survey: Staff Publications

Despite advances in toxicity testing and the development of new approach methodologies (NAMs) for hazard assessment, the ecological risk assessment (ERA) framework for terrestrial wildlife (i.e., air‐breathing amphibians, reptiles, birds, and mammals) has remained unchanged for decades. While survival, growth, and reproductive endpoints derived from whole-animal toxicity tests are central to hazard assessment, nonstandard measures of biological effects at multiple levels of biological organization (e.g., molecular, cellular, tissue, organ, organism, population, community, ecosystem) have the potential to enhance the relevance of prospective and retrospective wildlife ERAs. Other factors (e.g., indirect effects of contaminants on food supplies and infectious disease processes) …

Biological Case Against Downlisting The Whooping Crane And For Improving Implementation Under The Endangered Species Act, Andrew J. Caven, Hillary L. Thompson, David M. Baasch, Barry K. Hartup, Amanda M. Hegg, Stephanie M. Schmidt, Irvin Louque, Craig R. Allen, Carter G. Crouch, Craig A. Davis, Joel G. Jorgensen, Jane E. Austin, Bethany L. Ostrom, Richard D. Beilfuss, George W. Archibald, Anne E. Lacy

School of Natural Resources: Faculty Publications

The Whooping Crane (Grus americana; WHCR) is a large, long-lived bird endemic to North America. The remnant population migrates between Aransas National Wildlife Refuge, USA, and Wood Buffalo National Park, Canada (AWBP), and has recovered from a nadir of 15-16 birds in 1941 to ~540 birds in 2022. Two ongoing reintroduction efforts in Louisiana and the Eastern Flyway together total ~150 birds. Evidence indicates the U.S. Fish and Wildlife Service (USFWS) is strongly considering downlisting the species from an endangered to a threatened status under the Endangered Species Act (ESA). We examined the current status of the WHCR through the …

Quantitative Risk Assessment Of Oocyst Versus Bradyzoite Foodborne Transmission Of Toxoplasma Gondii In Brazil, Sophie Zhu, Elizabeth Vanwormer, Beatriz Martínez-López, Lílian Maria Garcia Bahia-Oliveira, Renato Augusto Damatta, Pedro Souto Rodrigues, Karen Shapiro

School of Veterinary and Biomedical Sciences: Faculty Publications

Toxoplasma gondii is a globally distributed zoonotic protozoan parasite. Infection with T. gondii can cause congenital toxoplasmosis in developing fetuses and acute outbreaks in the general population, and the disease burden is especially high in South America. Prior studies found that the environmental stage of T. gondii, oocysts, is an important source of infection in Brazil; however, no studies have quantified this risk relative to other parasite stages. We developed a Bayesian quantitative risk assessment (QRA) to estimate the relative attribution of the two primary parasite stages (bradyzoite and oocyst) that can be transmitted in foods to people in …

Students’ Knowledge Of And Attitudes Toward Dairy Production: A Survey Methodology Report, Heather Akin, Babatope Akinyemi, Julia Mcquillan, Tami Brown-Brandl

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

This report presents findings from a pilot survey conducted among undergraduate and graduate students (N = 410) at the University of Nebraska-Lincoln about students’ perspectives on technology usage, consumption, and sustainability in dairy production systems. An interdisciplinary research team developed the survey instrument and report. The main purpose of this pilot study was to create and administer survey items to support further research on experiential education and outreach opportunities related to robotics in small-scale dairy production and rural economic development. Descriptive findings indicated that most students had some familiarity with dairy production and the nutritional aspects of dairy products …

Peer Review Of Teaching: Et Theory, Deficit Irrigation And Consumptive Use, Derek M. Heeren, Saleh Taghvaeian

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A BSE Peer Review of Teaching was carried out for a graduate level course on Advanced Irrigation Management. This course has been offered for several years; each time improvements are made, but there is always a need for additional improvement. In particular, ET theory, deficit irrigation, and consumptive use are topics that the students struggle to grasp. These topics involve a relatively high level of math for an MS-level (800-level) Agricultural Systems Technology course, and the available materials on these topics either were not well developed or were not a good fit for this class. The primary objective was to …

Westward Expansion Of The Evening Bat (Nycticeius Humeralis) Into Dawson And Lincoln Counties Of Central Nebraska, Jamila Bajelan, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Evening Bat (Nycticeius humeralis) is a small, migratory species of temperate woodlands in the eastern United States. Along its western distributional edge in the Great Plains, this species resides in wooded riverine corridors. In recent decades, the distribution of Evening Bats has expanded westward throughout the Great Plains. Herein, we report on two county records that further document the distributional expansion of Evening Bats into central Nebraska. In 2019 and 2022, Evening Bats were collected in Lincoln and Dawson counties, respectively, in woodlands adjacent to the Platte River. Increases in wooded habitats along prairie waterways likely has …

Potassium Ion Channels As A Molecular Target To Reduce Virus Infection And Mortality Of Honey Bee Colonies, Christopher J. Fellows, Michael Simone‑Finstrom, Troy D. Anderson, Daniel R. Swale

Department of Entomology: Faculty Publications

Declines in managed honey bee populations are multifactorial but closely associated with reduced virus immunocompetence and thus, mechanisms to enhance immune function are likely to reduce viral infection rates and increase colony viability. However, gaps in knowledge regarding physiological mechanisms or ‘druggable’ target sites to enhance bee immunocompetence has prevented therapeutics development to reduce virus infection. Our data bridge this knowledge gap by identifying ATP-sensitive inward rectifier potassium ( K_ATP) channels as a pharmacologically tractable target for reducing virus-mediated mortality and viral replication in bees, as well as increasing an aspect of colony-level immunity. Bees infected with Israeli …

The Complete Mitochondrial Genome Of Eulaelaps Huzhuensis (Mesostigmata: Haemogamasidae), Hui-Juan Yang, Zhi-Hua Yang, Tian-Guang Ren, Wen-Ge Dong

Harold W. Manter Laboratory of Parasitology: Library Materials

Some mites of the family Haemogamasidae can transmit a variety of zoonotic diseases and have important public health and safety implications. Currently, however, little attention has been paid to molecular data of Haemogamasidae species, limiting our understanding of their evolutionary and phylogenetic relationships. In this study, the complete mitochondrial genome of Eulaelaps huzhuensis was determined for the first time, and its genomic information was analyzed in detail. The mitochondrial genome of E. huzhuensis is 14,872 bp in length with 37 genes and two control regions. The base composition showed a distinct AT preference. Twelve protein-coding genes have a typical ATN …

Type Iv Pilus-Mediated Inhibition Of Acinetobacter Baumannii Biofilm Formation By Phenothiazine Compounds, Nam Vo, Benjamin S. Sidner, Yafan Yu, Kurt H. Piepenbrink

Department of Biochemistry: Faculty Publications

Infections by pathogenic Acinetobacter species represent a significant burden on the health care system, despite their relative rarity, due to the difficulty of treating infections through oral antibiotics. Multidrug resistance is commonly observed in clinical Acinetobacter infections and multiple molecular mechanisms have been identified for this resistance, including multidrug efflux pumps, carbapenemase enzymes, and the formation of bacterial biofilm in persistent infections. Phenothiazine compounds have been identified as a potential inhibitor of type IV pilus production in multiple Gram-negative bacterial species. Here, we report the ability of two phenothiazines to inhibit type IV pilus-dependent surface (twitching) motility and biofilm formation …

Cover Crops And Soil Health In Rainfed And Irrigated Corn: What Did We Learn After 8 Years?, Humberto Blanco-Canqui, Sabrina J. Ruis, Katja Koehler-Cole, Roger Elmore, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Duration of cover crop (CC) management, CC biomass production, and other factors could impact how CC affects soil health. We studied the 8-year cumulative impacts of winter rye (Secale cereale L.) CC on soil physical, chemical, and biological properties in rainfed and irrigated no-till corn (Zea mays L.)-based systems in the western US Corn Belt. Average annual CC biomass production was 0.56 ± 0.51 Mg ha⁻¹ at the rainfed site and 0.98 ± 0.95 Mg ha⁻¹ at the irrigated site. After 8 years, CC improved particulate organic matter (POM) and mean weight diameter of waterstable aggregates …

Oral Microbial Communities In Children, Caregivers, And Associations With Salivary Biomeasures And Environmental Tobacco Smoke Exposure, Jason A. Rothman, Jenna L. Riis, Katrina R. Hamilton, Clancy Blair, Douglas A. Granger, Katrine Katrine Whiteson

Department of Psychology: Faculty Publications

Human oral microbial communities are diverse, with implications for oral and systemic health. Oral microbial communities change over time; thus, it is important to understand how healthy versus dysbiotic oral microbiomes differ, especially within and between families. There is also a need to understand how the oral microbiome composition is changed within an individual including by factors such as environmental tobacco smoke (ETS) exposure, metabolic regulation, inflammation, and antioxidant potential. Using archived saliva samples collected from caregivers and children during a 90-month follow-up assessment in a longitudinal study of child development in the context of rural poverty, we used 16S …

Editorial: New Insights Into Mechanisms Of Epigenetic Modifiers In Plant Growth And Development, Volume Ii, Ming Luo, Tomasz Jacek Sarnowski, Marc Libault, Gabino Ríos, Jean-Benoit Charron, Nitin Mantri, Shoudong Zhang

Department of Agronomy and Horticulture: Faculty Publications

As we have learned, chromatin modifications, including histone modifications and DNA methylation, play a key role in plant development (Ng and Bird, 1999). However, accumulated evidence shows that, besides chromatin biochemical modifications, other epigenetic regulations such as chromatin architecture also function at a pivot point to regulate plant development (Zhang et al., 2021). In this Research Topic, five research papers describe multiple developmental facets mediated by different epigenetic mechanisms besides histone modifications. These manuscripts report experimental evidence or summarize recent advances in epigenetic regulations of some important developmental genes or development-related mechanisms. This Research Topic allows readers to learn of …

Impact Of Land Use/Cover Change And Slope Gradient On Soil Organic Carbon Stock In Anjeni Watershed, Northwest Ethiopia, Bethel Geremew, Tsegaye Tadesse, Bobe Bedadi, Hero T. Gollany, Kindie Tesfaye, Abebe Aschalew

National Drought Mitigation Center: Faculty Publications

Today’s agri-food systems face the triple challenge of addressing food security, adapting to climate change, and reducing the climate footprint by reducing the emission of greenhouse gases (GHG). In agri-food systems, changes in land use and land cover (LULC) could affect soil physicochemical properties, particularly soil organic carbon (SOC) stock. However, the impact varies depending on the physical, social, and economic conditions of a given region or watershed. Given this, a study was conducted to quantify the impact of LULC and slope gradient on SOC stock and C sequestration rate in the Anjeni watershed, which is a highly populated and …

Maize Nitrogen Uptake, Grain Nitrogen Concentration And Root-Zone Residual Nitrate Nitrogen Response Under Center Pivot, Subsurface Drip And Surface (Furrow) Irrigation, Suat Irmak, Ali T. Mohammed, Matthew Drudik

Department of Agricultural and Biological Systems Engineering: Faculty Publications

While the impact(s) of irrigation and nitrogen (N) levels on crop yields have been investigated separately, research lacks about how different irrigation methods coupled with different N application timing strategies under full and limited irrigation levels effect yield, soil nitrate nitrogen (NO₃-N) residual and stover N uptake. Knowledge of these dynamics can help to establish effective water and N management guidelines for the same crop under different irrigation methods and irrigation levels. This research investigated maize (Zea mays L.) residual soil NO₃-N concentration and stover and grain N uptake response to water under different irrigation …

Influence Of Maternal Dietary Protein Precursor On Reproductive Endocrinology Of Neonatal Bovine Offspring, Jennifer F. Thorson, Ligia D. Prezotto

Department of Animal Science: Faculty Publications

Bovine oogenesis and follicular maturation are initiated during in utero development, a process that is stringently orchestrated by the endocrine milieu. Of paramount importance are the three major biologically active estrogens: estron, estradiol, and estriol that are involved in the development and maintenance of germ cells. Previous work using the current model have revealed that maternal urea (protein precursor) supplementation reduces the ovarian follicular reserve without compromising the number of secondary and tertiary follicles. Therefore, we did not anticipate an influence of dietary treatment on biologically active estrogens. However, as the ovarian reserve is positively associated with circulating concentrations of …

Ten Principles For Bird-Friendly Forestry: Conservation Approaches In Natural Forests Used For Timber Production, Nico Arcilla, Māris Strazds

Center for Great Plains Studies: Staff and Fellows Publications

Bird–forestry relationships have been the subject of research and conservation initiatives for decades, but there are few reviews of resulting recommendations for use by forest managers. We define “bird-friendly forestry” as forest management that applies recommendations from research seeking to reconcile logging with bird conservation in natural forests used for timber production. We reviewed relevant studies to synthesize 10 principles of bird-friendly forestry: (1) protect and enhance vertical structure through uneven-aged silviculture; (2) leave abundant dead wood in different decay stages; (3) maintain residual large green trees; (4) create and maintain sufficient amounts of uncut reserves and corridors; (5) maximize …

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence

Department of Animal Science: Faculty Publications

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, …

Restoration Of Normal Blood Flow In Atherosclerotic Arteries Promotes Plaque Stabilization, Morgan A. Schake, Ian Mccue, Evan T. Curtis, Thomas J. Ripperda Jr., Samuel Harvey, Bryan T. Hackfort, Anna Fitzwater, Yiannis S. Chatzizisis, Forrest Kievit, Ryan M. Pedrigi

Department of Mechanical and Materials Engineering: Faculty Publications

Blood flow is a key regulator of atherosclerosis. Disturbed blood flow promotes atherosclerotic plaque development, whereas normal blood flow protects against plaque development. We hypothesized that normal blood flow is also therapeutic, if it were able to be restored within atherosclerotic arteries. Apolipoprotein E-deficient (ApoE^-/-) mice were initially instrumented with a blood flow-modifying cuff to induce plaque development and then five weeks later the cuffwas removed to allowrestoration of normal blood flow. Plaques in decuffed mice exhibited compositional changes that indicated increased stability compared to plaques in mice with the cuff maintained. The therapeutic benefit of decuffingwas comparable …

Protocol To Develop A Synthetic Biology Toolkit For The Non-Model Bacterium R. Palustris, Mark Kathol, Cheryl Immethun, Rajib Saha

Department of Chemical and Biomolecular Engineering: Faculty Publications

Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in nonmodel bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al..¹

A Complete Telomere-To-Telomere Assembly Of The Maize Genome, Jian Chen, Zijian Wang, Kaiwen Tan, Wei Huang, Junpeng Shi, Tong Li, Jiang Hu, Kai Wang, Chao Wang, Beibei Xin, Haiming Zhao, Weibin Song, Matthew B. Hufford, James C. Schnable, Weiwei Jin, China Agricultural University, Hainan Yazhou Bay Seed Laboratory

Department of Agronomy and Horticulture: Faculty Publications

A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a complete genome assembly of maize with each chromosome entirely traversed in a single contig. The 2,178.6 Mb T2T Mo17 genome with a base accuracy of over 99.99% unveiled the structural features of all repetitive regions of the genome. There were several super-long simple-sequence-repeat arrays having consecutive thymine–adenine– guanine (TAG) tri-nucleotide repeats up to 235 kb. The assembly of the entire nucleolar organizer region of the 26.8 Mb array with …

Bat Use Of Afforested And Encroached Patches And Their Role In Extending Bat Habitat Into The Nebraska Sandhills, Jacob L. Wagner

School of Natural Resources: Dissertations, Theses, and Student Research

The Nebraska Sandhills are currently undergoing a state shift to a redcedar dominated woodland due to anthropologic planting of eastern redcedar (Juniperus virginiana) and woody encroachment from the periphery of the Sandhills. To better understand this novel ecosystem and how bats are utilizing it I collected data at Barta Brothers Ranch with acoustic sensor grids consisting of 24,100 m-spaced acoustic sensors placed adjacent to planted windbreaks. Supplemental data from the Nebraska North American Bat Monitoring Program was used for data analysis at larger spatial scales. I used linear regressions and kriging interpolation maps to see how bats used windbreaks during …

Preliminary Evidence Of Anticoagulant Rodenticide Exposure In American Kestrels (Falco Sparverius) In The Western United States, Evan R. Buechley, Dave Oleyar, Jesse L. Watson, Jennifer Bridgeman, Steven Volker, David A. Goldade, Catherine E. Swift, Barnett A. Rattner

United States Geological Survey: Staff Publications

Although there is extensive evidence of declines in the American Kestrel (Falco sparverius) population across North America, the cause of such declines remains a mystery. One hypothesized driver of decline is anticoagulant rodenticide (AR) exposure, which could potentially cause mortality or reduced fitness. We investigated AR exposure in wild American Kestrels in Utah, USA. We collected and tested for AR residues in liver samples (n = 8) from kestrels opportunistically encountered dead and in blood samples (n = 71) from live wild kestrels, both nestlings and adults. We found high detection rates in both tissues. Adult …

Genome-Wide Association Analysis For Emergence Of Deeply Sown Rice (Oryza Sativa) Reveals Novel Aus-Specific Phytohormone Candidate Genes For Adaptation To Dry-Direct Seeding In The Field, Sandeep A. Sakhale, Shailesh Yadav, Lindsay V. Clark, Alexander E. Lipka, Arvind Kumar, Erik J. Sacks

Department of Agronomy and Horticulture: Faculty Publications

Dry direct-seeded rice (dry-DSR) is typically sown deeply to circumvent the need for irrigation, and thus seedling emergence is a crucial trait affecting plant stand and yield. To breed elite cultivars that use less water and are climate-resilient, an understanding of the genomic regions and underlying genes that confer emergence in deeply sown dry-DSR would be highly advantageous. A combined diversity panel of 470 rice accessions (RDP1 plus aus subset of 3K RGP) was evaluated with 2.9 million single nucleotide polymorphisms (SNPs) to identify associations with dry-DSR traits in the field and component traits in a controlled-environment experiment. Using genome-wide …

Mycobacterium Avium Subsp. Paratuberculosis Candidate Vaccine Strains Are Pro-Apoptotic In Raw264.7murinemacrophages, Raul G. Barletta, John P. Bannantine, Judith R. Stabel, Ezhumalai Muthukrishnan, Dirk Anderson, Enakshy Dutta, Vamsi Manthena, Mostafa Hanafy, Denise K. Zinniel

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease, a severe gastroenteritis of ruminants. This study developed a model cell culture system to rapidly screen MAP mutants with vaccine potential for apoptosis. Two wild-type strains, a transposon mutant, and two deletion mutant MAP strains (MOI of 10 with 1.2 × 10⁶ CFU) were tested in murine RAW 264.7 macrophages to determine if they induce apoptosis and/or necrosis. Both deletion mutants were previously shown to be attenuated and immunogenic in primary bovine macrophages. All strains had similar growth rates, but cell morphology indicated that both deletion mutants …

Viral Dna Accumulation Regulates Replication Efficiency Of Chlorovirus Osy-Ne5 In Two Closely Related Chlorella Variabilis Strains, Ahmed Esmael, Irina V. Agarkova, David D. Dunigan, You Zhou, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Many chloroviruses replicate in Chlorella variabilis algal strains that are ex-endosymbionts isolated from the protozoan Paramecium bursaria, including the NC64A and Syngen 2-3 strains. We noticed that indigenous water samples produced a higher number of plaque-forming viruses on C. variabilis Syngen 2-3 lawns than on C. variabilis NC64A lawns. These observed differences led to the discovery of viruses that replicate exclusively in Syngen 2-3 cells, named Only Syngen (OSy) viruses. Here, we demonstrate that OSy viruses initiate infection in the restricted host NC64A by synthesizing some early virus gene products and that approximately 20% of the cells produce a …

Optical Characterization Of Isothermal Spin State Switching In An Fe(Ii) Spin Crossover Molecular And Polymer Ferroelectric Bilayer, Saeed Yazdani, Kourtney Collier, Grace Yang, Jared Phillips, Ashley Dale, Aaron Mosey, Samuel Grocki, Jian Zhang, Anne E. Shanahan, Ruihua Cheng, Peter A. Dowben

Department of Physics and Astronomy: Faculty Publications

Using optical characterization, it is evident that the spin state of the spin crossover molecular complex [Fe{H₂B(pz)₂}₂(bipy)] (pz = tris(pyrazol-1-1y)-borohydride, bipy = 2,2′-bipyridine) depends on the electric polarization of the adjacent polymer ferroelectric polyvinylidene fluoride-hexafluoropropylene (PVDF-HFP) thin film. The role of the PVDF-HFP thin film is significant but complex. The UV–Vis spectroscopy measurements reveals that room temperature switching of the electronic structure of [Fe{H₂B(pz)₂}₂(bipy)] molecules in bilayers of PVDF-HFP/[Fe{H₂B(pz)₂}₂(bipy)] occurs as a function of ferroelectric polarization. The retention of voltage-controlled nonvolatile changes …