Digital Commons Network^™

Essential And Recurrent Roles For Hairpin Rnas In Silencing De Novo Sex Chromosome Conflict In Drosophila Simulans, Jeffrey Vedanayagam, Marion Herbette, Holly Mudgett, Ching-Jung Lin, Chun- Ming Lai, Caitlin Mcdonough-Goldstein, Stephen Dorus, Benjamin Loppin, Colin D. Meiklejohn, Raphaëlle Dubruille, Eric C. Lai

School of Biological Sciences: Faculty Publications

Meiotic drive loci distort the normally equal segregation of alleles, which benefits their own transmission even in the face of severe fitness costs to their host organism. However, relatively little is known about the molecular identity of meiotic drivers, their strategies of action, and mechanisms that can suppress their activity. Here, we present data from the fruitfly Drosophila simulans that address these questions. We show that a family of de novo, protamine- derived X-linked selfish genes (the Dox gene family) is silenced by a pair of newly emerged hairpin RNA (hpRNA) small interfering RNA (siRNA)-class loci, Nmy and Tmy. …

Developing A Slow-Release Permanganate Composite For Degrading Aquaculture Antibiotics, Chainarong Sakulthaew, Chanat Chokejaroenrat, Sidaporn Panya, Apisit Songsasen, Kitipong Poomipuen, Saksit Imman, Nopparat Suriyachai, Torpong Kreetachat, Steven Comfort

School of Natural Resources: Faculty Publications

Copious use of antibiotics in aquaculture farming systems has resulted in surface water contamination in some countries. Our objective was to develop a slow-release oxidant that could be used in situ to reduce antibiotic concentrations in discharges from aquaculture lagoons. We accomplished this by generating a slow-release permanganate (SR-MnO₄^-) that was composed of a biodegradable wax and a phosphate-based dispersing agent. Sulfadimethoxine (SDM) and its synergistic antibiotics were used as representative surrogates. Kinetic experiments verified that the antibiotic-MnO₄^- reactions were first-order with respect to MnO₄^- and initial antibiotic concentration (second-order rates: 0.056–0.128 s …

Hematopoietic Stem Cells And Betaherpesvirus Latency, Lindsey B. Crawford

Department of Biochemistry: Faculty Publications

The human betaherpesviruses including human cytomegalovirus (HCMV), human herpesvirus (HHV)-6a and HHV-6b, and HHV-7 infect and establish latency in CD34+ hematopoietic stem and progenitor cells (HPCs). The diverse repertoire of HPCs in humans and the complex interactions between these viruses and host HPCs regulate the viral lifecycle, including latency. Precise manipulation of host and viral factors contribute to preferential maintenance of the viral genome, increased host cell survival, and specific manipulation of the cellular environment including suppression of neighboring cells and immune control. The dynamic control of these processes by the virus regulate inter- and intra-host signals critical to the …

Basic Fibroblast Growth Factor Induces Proliferation And Collagen Production By Fibroblasts Derived From The Bovine Corpus Luteum, Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Cyclic regression of the ovarian corpus luteum, the endocrine gland responsible for progesterone production, involves rapid matrix remodeling. Despite fibroblasts in other systems being known for producing and maintaining extracellular matrix, little is known about fibroblasts in the functional or regressing corpus luteum. Vast transcriptomic changes occur in the regressing corpus luteum, among which are reduced levels of vascular endothelial growth factor A (VEGFA) and increased expression of fibroblast growth factor 2 (FGF2) after 4 and 12 h of induced regression, when progesterone is declining and the microvasculature is destabilizing. We hypothesized that FGF2 activates luteal …

Movement Of Striacosta Albicosta (Smith) (Lepidoptera: Noctuidae) Larvae On Transgenic Bt And Non-Bt Maize, Débora G. Montezano, Thomas E. Hunt, Priscila M. Colombo Da Luz, Kelsey Karnik, Stephen D. Kachman, Ana Maria Velez, Julie A. Peterson

Department of Entomology: Faculty Publications

Exposure of lepidopteran pests to Bacillus thuringiensis (Bt) proteins has been shown to affect the behavior of larvae, including increased movement and avoidance of Bt-expressing plants or diet. Therefore, we hypothesized that the behavior of western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), an important pest of maize, could be affected when exposed to Bt plants. To test this hypothesis, we conducted a series of artificial arena and on-plant experiments to determine S. albicosta neonate behavior when exposed to Bt and non-Bt plant tissue. Video tracking experiments presented neonate larvae with the choice of Bt or non- …

Coparenting Support In The Context Of Difficult Children: Mother And Father Differences, Victoria J. Johnson, Dongho Choi, Lorey A. Wheeler, Patty X. Kuo

Department of Child, Youth, and Family Studies: Faculty Publications

While parenting children with difficult behaviors can intensify stress within the entire family system, families may lean on other familial relationships to mitigate that stress. The coparenting relationship is known to play a key role within the family system for child outcomes and familial interactions, but it is not clear whether it eases the stress and challenge of raising a difficult child, nor how that plays out differently for mothers versus fathers. Ninety-six couples (89.7% married) parenting young children (Mean age = 3.22 years) were included in this study. Using cross-sectional and aggregated daily response data, actor–partner interdependence models were …

Spatial And Temporal Activity Patterns Among Sympatric Tree-Roosting Bat Species In An Agriculturally Dominated Great Plains Landscape, Christopher Fill, Craig R. Allen, John F. Benson, Dirac Twidwell

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

In agroecosystems, bats can provide a critical ecosystem service by consuming night-flying insect pests. However, many bats also face intense population pressures from human landscape modification, global change and novel diseases. To better understand the behavioral activity of different bat species with respect to space, time, habitat, and other bat species in this environment, we investigated species correlations in space and time over row crop agricultural fields. We used acoustic grids to document spatial and temporal co-occurrence or avoidance between bats and recorded eight species across the 10 field sites we sampled. All species significantly overlapped in two-dimensional space and …

Curated And Harmonized Gut Microbiome 16s Rrna Amplicon Data From Dietary Fiber Intervention Studies In Humans, Cynthia I. Rodriguez, Ali Keshavarzian, Bruce R. Hamaker, Feitong Liu, Genelle R. Lunken, Heather Rasmussen, Hongwei Zhou, Julien Tap, Kelly S. Swanson, Maria Ukhanova, Marion Leclerc, Martin Gotteland, Paola Navarrete, Petia Kovatcheva-Datchary, Wendy J. Dahl, Jennifer B. H. Martiny

Department of Nutrition and Health Sciences: Faculty Publications

Next generation amplicon sequencing has created a plethora of data from human microbiomes. The accessibility to this scientific data and its corresponding metadata is important for its reuse, to allow for new discoveries, verification of published results, and serving as path for reproducibility. Dietary fiber consumption has been associated with a variety of health benefits that are thought to be mediated by gut microbiota. To enable direct comparisons of the response of the gut microbiome to fiber, we obtained 16S rRNA sequencing data and its corresponding metadata from 11 fiber intervention studies for a total of 2,368 samples. We provide …

Family Strengths Among Native American Families And Families Living In Poverty: Preventing Adverse Childhood Experiences, Natira Mullet, Emily A. Waterman, Katie Edwards, Briana Simon, Skyler Hopfauf, Ramona Herrington

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

Objective: The purpose of this study was to understand how youth, caregivers, and community professionals perceive family strengths and adverse childhood experiences (ACEs) in their community. Specifically, this study was focused on the protective role of caregivers and families, positive youth development, and how Native American families and families living in poverty support adolescents’ social–emotional development and help them thrive in the face of adversity.

Background: Research documents the concerning rates and negative outcomes of ACEs. However, very little research has examined the views of families and professionals on how to prevent ACES among these populations.

Method: Participants were youth …

Realizing Molecular Machine Learning Through Communications For Biological Ai: Future Directions And Challenges, Sasitharan Balasubramaniam, Samitha Somathilaka, Sehee Sun, Adrian Ratwatte, Massimiliano Pierobon

School of Computing: Faculty Publications

Artificial Intelligence (AI) and Machine Learning (ML) are weaving their way into the fabric of society, where they are playing a crucial role in numerous facets of our lives. As we witness the increased deployment of AI and ML in various types of devices, we benefit from their use into energy-efficient algorithms for low powered devices. In this paper, we investigate a scale and medium that is far smaller than conventional devices as we move towards molecular systems that can be utilized to perform machine learning functions, i.e., Molecular Machine Learning (MML). Fundamental to the operation of MML is the …

Nou Spring Field Days And Annual Meeting. Valentine, May 5-7, 2023

Nebraska Bird Review

The NOU Annual Meeting and Spring Field Days was held in Valentine on May 5-7, 2023, with 50 in attendance. Our meetings and meals took place in the Niobrara Lodge. Field trips were led by Robin Harding, William Flack, T. J. Walker, Dave Heidt, Mark Brogie and Don and Janis Paseka. Destinations included Thomas Creek WMA, Cub Creek Recreation Area, the Niobrara Valley Preserve (TNC Property), the river road from Meadville (SW of Springview) to Norden, the Valentine City Park, Valentine Fish Hatchery, Ft. Niobrara NWR, Anderson Bridge WMA, the Minnechaduza Creek crossing north of Kilgore, Steer Creek, Merritt Reservoir, …

Physician Aid In Dying: Physician-Assisted Suicide As A Constitutionally Protected Liberty Interest Under The Nebraska Constitution, Emily Newcomb

Nebraska Law Review

“Every life is different from any that has gone before it, and so is every death.”1 Although advancements in technology and healthcare have saved and prolonged countless lives, there is one part of humanity that can never be fully cured: death. Death is not what it used to be—it is no longer normally a common, family communal, or even religious event.2 Modern dying takes place in a hospital while attached to machines that attempt to prolong life but seem to simultaneously take patients further and further away from dying with dignity. As these technologies that prolong life evolve, so do …

Expanding State Parent Registry Laws, Jeffrey A. Parness

Nebraska Law Review

As with state recognized voluntary acknowledgements of parentage (VAPs) and state recognized assisted reproduction pacts (SRARPs) on childcare parentage for future or current children, state parent registries (PRs), often labeled putative paternity registries or putative father registries, embody declarations of expecting or current legal parenthood.1 Yet declarations on children in PRs often involve unilateral assertions, unlike dual parenthood declarations in VAPs. Actual parenthood under law for many PR declarants is never recognized because there are no simultaneous assertions by a second expecting or existing legal parent on the declarant’s parenthood, as with an assertion by an expecting or existing birth …

Rethinking The Status And Temporal Occurrence Of The American Woodcock In Nebraska Because Of Its Unusual Life History, Joel G. Jorgensen, Stephen J. Brenner

Nebraska Bird Review

Historical distribution and range expansion -- We reviewed existing information to evaluate conclusions by previous authors (e.g., Sharpe et al. 2001, Silcock and Jorgensen 2023) that woodcock have expanded their range west since settlement of Nebraska by Euro-Americans. We also provide additional context about the species’ distribution over time using regional resources.

Temporal occurrence and abundance -- We used data from woodcock outfitted with satellite transmitters 2021-2022 and compared it with occurrence data from two sources, eBird (eBird.org 2023) from all years through 2022 and the SFRs in the Nebraska Bird Review 1994–2019. We chose not to use SFR data …

Spring Field Report, March - May 2023, W. Ross Silcock

Nebraska Bird Review

First off, I want to reiterate that reports compiled for this Spring Seasonal Report are evaluated against current knowledge as presented in Birds of Nebraska-Online (“BONO”; https://birds.outdoornebraska.gov/). Species whose reports do not add new information on distribution, early or late dates, etc., are listed as having “Routine reports”. Please let me know at [email protected] if you have information that is not in BONO, or if you have additions and/or corrections of information in BONO. Another reminder: if evidence of breeding is observed, please enter the appropriate descriptor in your eBird checklist under “Breeding and Behavior Code”. These descriptors can be …

The Nebraska Bird Review: A Magazine Of Ornithology Of The Nebraska Region. Volume 91 June 2023 Number 2

Nebraska Bird Review

Spring Field Report, March - May 2023 by W. Ross Silcock …46

Correction to 2022-2023 Christmas Bird Count tally ….70

Rethinking the Status and Temporal Occurrence of the American Woodcock in Nebraska because of its Unusual Life History by Joel G. Jorgensen and Stephen J. Brenner …71

NOU Spring Field Days and Annual Meeting in Valentine, May 5-7, 2023 …..84

Subscription and Organization Information …91

Reinterpreting The Ministerial Exception In Our Lady Of Guadalupe School V. Morrissey-Berru, Robert Drust

Nebraska Law Review

Employees enjoy protections under federal anti-discrimination statutes from adverse employment action or retaliation against them based on protected classes, such as disability, race, sex, and religion. Title VII of the Civil Rights Act prevents discrimination in employment based on race, color, sex, national origin, or religion.1 It also creates an exemption for religious institutions to discriminate in employment based on religion.2 Unlike Title VII, other anti-discrimination statutes do not carry this religious exemption.3 As a result, courts have created methods for determining whether a religious institution is exempt from these statutes. One such way is the “ministerial exception” fashioned out …

Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor (L.) Moench) is a versatile and prosperous feedstock crop for renewable energy production. With the increase in bioenergy demand, the production of higher oil content per biomass in a feedstock crop is a highly desirable trait for the biofuel conversion process yield. Especially if this outcome can be achieved using fewer inputs in the field, such as nitrogen. In microalgae species, nitrogen limitation has been associated with changes in the carbon storage metabolic pathway favoring triacylglycerol (TAG) accumulation. In this context, this study aimed to assess whether nitrogen starvation would result in a similar outcome in …

Synthesis And Study Of High-Spin Stable Organic Radicals For Electrical Conductors And Mannosamine Nitroxide For Mri Contrast Agents, Shuyang Zhang

Department of Chemistry: Dissertations, Theses, and Student Research

In the first project, we describe the synthesis of an ambient stable high spin organic diradical 4 based on the Blatter moiety. The high-spin (S = 1) organic diradical 4, which consists of two Blatter radical moieties in a conjugated structure, exhibits a nearly exclusive population (88%) on triplet ground state at room temperature as a consequence of a large single-triplet energy gap (ΔEST = 0.5 kcal/mol). The target diradical molecule is synthesized over five steps with structural confirmation by single-crystal X-ray diffraction. The thermogravimetric analysis (TGA) shows the onset of decomposition at ~264 oC, indicating the diradical molecule has …

Intensification Differentially Affects The Delivery Of Multiple Ecosystem Services In Subtropical And Temperate Grasslands, Shishir Paudel, Nuria Gomez-Casanovas, Elizabeth H. Boughton, Samuel D. Chamberlain, Pradeep Wagle, Brekke L. Peterson, Rajen Bajgain, Patrick J. Starks, Jefferey Basara, Carl J. Bernacchi, Evan H. Delucia, Laura E. Goodman, Prasanna H. Gowda, Ryan Reuter, Jed P. Sparks, Hilary M. Swain, Xiangming Xiao, Jean L. Steiner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Intensification, the process of intensifying land management to enhance agricultural goods, results in “intensive” pastures that are planted with productive grasses and fertilized. These intensive pastures provide essential ecosystem services, including forage production for livestock. Understanding the synergies and tradeoffs of pasture intensification on the delivery of services across climatic regions is crucial to shape policies and incentives for better management of natural resources. Here, we investigated how grassland intensification affects key components of provisioning (forage productivity and quality), supporting (plant diversity) and regulating services (CO2 and CH4 fluxes) by comparing these services between intensive versus extensive pastures in subtropical …

Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Rationale and Purpose — Adding multispecies cover crop (CC) mixtures could diversify the current simplified crop rotations and enhance soil health more than monoculture CCs. Further, CC mixtures with diverse plant species could adapt better to changing climatic and environmental conditions than monoculture CCs. However, our current understanding of the soil benefits of CC mixtures is still limited. This review discussed whether CC mixtures are better than monoculture CCs to improve soil physical health.

Methods — All studies published up to May 25, 2023, comparing soil physical properties between CC mixtures and their constituents grown as monocultures were searched in …

Plestiodon Egregius Insularis (Cedar Key Mole Skink). Reproduction., Jake Scott, Kevin M. Enge, Louis A. Somma, Richard D. Bartlett

Papers in Herpetology

PLESTIODON EGREGIUS INSULARlS (Cedar Key Mole Skink). REPRODUCTION. Plestiodon egregius insularis is the largest of five described subspecies (Mount 1965. Bull. Florida St. Mus. BioI. Sci. 9:183-213) and is known from only nine small islands off the coast of Levy County, Florida, USA. The only documented clutch size for P. e. insularis contained five eggs (Mount 1963. Am. MidI. Nat. 70:356-385). For wild P. egregius, Mount (1963, op. cit.) reported 2-9 (mean = 4.8) eggs for 13 clutches, and Hamilton and Pollack (1958. Herpetologica 14:25-28) found two nests of P. e. similis in Georgia containing five eggs each. In captivity, …

Evaluating Assessment Score Validity And Characterizing Undergraduate Biology Exam Content, Crystal Uminski

School of Biological Sciences: Dissertations, Theses, and Student Research

The landscape of undergraduate biology education has been shaped by decades of reform efforts calling for instruction to integrate core concepts and scientific skills as a means of helping students become proficient in the discipline. Assessments can be used to make inferences about how these reform efforts have translated into changes in department curriculum and course practices. Such changes can be measured using student scores on researcher-developed programmatic and concept assessments. Scores on these assessments are often assumed to be accurate representations of student biology content knowledge, but my work indicates that the validity of these interpretations may be threatened …

The Open Access Journals Toolkit, Alex Mendonça, Andrea Chiarelli, Andy Byers, Andy Nobes, Chris Hartgerink, Clarissa França Dias Carneiro, Elle Malcolmson, Ivonne Lijano, Katie Foxall, Lucia Loffreda, Rebecca Wojturska, Solange Santos, Susan Murray, Tom Olijhoek, Wendy Patterson

Copyright, Fair Use, Scholarly Communication, etc.

Contents: Getting Started 5 • Scope, aims and focus 5 • Choosing a title for your journal 6 • Types of content accepted 7 • Kick-off and ongoing funding 11 • Disciplinary considerations 16 • Journal setup checklist and timeline 18 • Running a journal 20 • Article selection criteria 20 • Publication frequency and journal issues 23 • Attracting authors 25 • Peer review and quality assurance 27 • The costs of running an online open access journal 31 • Running a journal in a local or regional language 34 • Flipping a journal to open access 36 • …

First Course Portfolio For Bioc205: Scientific Analysis And Technical Writing, Lindsey B. Crawford

UNL Faculty Course Portfolios

BIOC205: Scientific Analysis and Technical Writing is a core course for the Department of Biochemistry at the University of Nebraska-Lincoln. This course is geared for second year (sophomore students) with minimal prerequisites and as a foundation for future classes. Challenges include 1) teaching the needed scientific background and elements of scientific analysis and writing to a broad cohort of students with diverse backgrounds and interests, 2) building student skills towards analysis of primary peer-reviewed scientific literature, 3) developing students as writers of scientific information for different audiences. This course used Genetic Engineering as a broad topic choice to capture students …

Integration Of Environmental Sustainability And Decision Making: Case Studies Of Civil Infrastructure, Sussan Moussavi

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Sustainable development is important in the planning and design of critical civil infrastructure systems. However, decision making related to these systems currently lacks meaningful sustainability considerations. To fill this gap, this research explores the life cycle impacts of wastewater and energy infrastructure using case studies, life cycle assessment methodologies, and stakeholder discussions. Environmental and economic impacts, which are more commonly quantified, as well as more difficult to quantify societal factors, are explored. The goal of this research is to help inform decision making and facilitate the consideration of the three pillars of sustainability (e.g., economic, environmental, and social) during the …

Engineering A Multicompartment In Vitro Model For Dorsal Root Ganglia Phenotypic Assessment, Sydney M. Caparaso, Adan Redwine, Rebecca A. Wachs

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Despite the significant global prevalence of chronic pain, current methods to identify pain therapeutics often fail translation to the clinic. Phenotypic screening platforms rely on modeling and assessing key pathologies relevant to chronic pain, improving predictive capability. Patients with chronic pain often present with sensitization of primary sensory neurons (that extend from dorsal root ganglia [DRG]). During neuronal sensitization, painful nociceptors display lowered stimulation thresholds. To model neuronal excitability, it is necessary to maintain three key anatomical features of DRGs to have a physiologically relevant platform: (1) isolation between DRG cell bodies and neurons, (2) 3D platform to preserve cell–cell …

Toxicological Effects Assessment For Wildlife In The 21st Century: Review Of Current Methods And Recommendations For A Path Forward, Thomas G. Bean, Val R. Beasley, Philippe Berny, Karen M. Eisenreich, John E. Elliott, Margaret L. Eng, Phyllis C. Fuchsman, Mark S. Johnson, Mason D. King, Rafael Mateo, Carolyn B. Meyer, Christopher J. Salice, Barnett A. Rattner

United States Geological Survey: Staff Publications

Model species (e.g., granivorous gamebirds, waterfowl, passerines, domesticated rodents) have been used for decades in guideline laboratory tests to generate survival, growth, and reproductive data for prospective ecological risk assessments (ERAs) for birds and mammals, while officially adopted risk assessment schemes for amphibians and reptiles do not exist. There are recognized shortcomings of current in vivo methods as well as uncertainty around the extent to which species with different life histories (e.g., terrestrial amphibians, reptiles, bats) than these commonly used models are protected by existing ERA frameworks. Approaches other than validating additional animal models for testing are being developed, but …

Two New Genera Of Enopliinae, Skelleyus And Divulgoatus (Coleoptera: Cleridae), Weston Opitz

Insecta Mundi

Two new genera, Skelleyus Opitz and Divulgoatus Opitz (Coleoptera: Cleridae: Enopliinae), show unusual antennal development. Funicular antennomeres become shorter as they approach an extensive ca­pitulum. This study involves three taxa, Skelleyus leavengoodi Opitz, new species, Divulgoatus kelleri Opitz, new species, and Divulgoatus discrepans (Gorham).

The two new genera of Cleridae presented in this work show a most unusual antennal development. The greatly extended antennal capitulum is not found in any other member of the Enopliinae. The complete illustrations of the antennae are depicted in the species descriptions.

Soybean Crop Coefficients Under Different Seeding Rates And Full And Limited Irrigation And Rainfed Management, Suat Irmak, Rupinder Sandhu

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effects of soybean seeding rates on evapotranspiration (ET_c) and grassand alfalfa-reference crop coefficients (K_co and K_cr) were investigated under five seeding rates (185,250, 247,000, 308,750, 370,500 and 432,250 seeds ha^-1) (62, 82, 103, 124 and 144% of the recommended seeding rate of 300,000 seeds ha^-1 in the experimental region) and different irrigation strategies (i.e. full irrigation treatment [FIT], limited irrigation of 75% FIT and 50% FIT and rainfed treatment). K_co and K_cr values were developed for each soybean growth stage. The seasonal ET_c ranged from 460 mm for …