Digital Commons Network^™

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …

Radiofrequency Inactivation Of Salmonella In Black Pepper And Dried Basil Leaves Using In-Package Steaming, Surabhi Wason, Rossana Villa-Rojas, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

Radiofrequency (RF) heating has been extensively studied for pasteurizing low‐moisture foods. Currently, bulk foods are treated with radiofrequency; potential cross‐contamination may occur during packaging of pasteurized products. As an alternative, in‐package RF processing was evaluated for Salmonella inactivation on black peppercorns and dried basil leaves and prevention of cross‐contamination during storage postprocessing. Inpackage steaming refers to the process in which the samples were heated in a steam vent package to generate and retain steam during the treatment. This treatment achieved good heating uniformity which could be because of the circulation of steam within the package. One‐way steam vent allowed the …

Pathogenicity In Chickens And Turkeys Of A 2021 United States H5n1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b Wild Bird Virus Compared To Two Previous H5n8 Clade 2.3.4.4 Viruses, Mary J. Pantin-Jackwood, Erica Spackman, Christina Leyson, Sungsu Youk, Scott A. Lee, Linda M. Moon, Mia K. Torchetti, Mary L. Killian, Julianna B. Lenoch, Darrell R. Kapczynski, David E. Swayne, David L. Suarez

United States Department of Agriculture Wildlife Services: Staff Publications

Highly pathogenic avian influenza viruses (HPAIVs) of subtype H5 of the Gs/GD/96 lineage remain a major threat to poultry due to endemicity in wild birds. H5N1 HPAIVs from this lineage were detected in 2021 in the United States (US) and since then have infected many wild and domestic birds. We evaluated the pathobiology of an early US H5N1 HPAIV (clade 2.3.4.4b, 2021) and two H5N8 HPAIVs from previous outbreaks in the US (clade 2.3.4.4c, 2014) and Europe (clade 2.3.4.4b, 2016) in chickens and turkeys. Differences in clinical signs, mean death times (MDTs), and virus transmissibility were found between chickens and …

Enzyme Kinetics Of Deoxyuridine Triphosphatase From Western Corn Rootworm, Carlos Riera‑Ruiz, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

Objective The Western corn rootworm (WCR), Diabrotica virgifera virgifera, is a highly adaptable insect pest that has evolved resistance to a variety of control strategies, including insecticides. Therefore, it is interesting to examine how housekeeping proteins in WCR have been changed under WCR-controlling strategies. In this study, we focused on one of such proteins in WCR, a ubiquitous enzyme 5’-triphosphate nucleotidohydrolase (dUTPase). In the thymidine synthetic pathway, dUTPase hydrolyzes deoxyuridine triphosphate (dUTP) and supplies the substrate, deoxyuridine monophosphate, for the thymidylate synthase (TS). It decreases the cellular content of uracil, reducing uracil misincorporation into DNA. Suppressing the dUTPase activity, …

Enzyme Kinetics Of Deoxyuridine Triphosphatase From Western Corn Rootworm, Carlos Riera‑Ruiz, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

Objective The Western corn rootworm (WCR), Diabrotica virgifera virgifera, is a highly adaptable insect pest that has evolved resistance to a variety of control strategies, including insecticides. Therefore, it is interesting to examine how housekeeping proteins in WCR have been changed under WCR-controlling strategies. In this study, we focused on one of such proteins in WCR, a ubiquitous enzyme 5’-triphosphate nucleotidohydrolase (dUTPase). In the thymidine synthetic pathway, dUTPase hydrolyzes deoxyuridine triphosphate (dUTP) and supplies the substrate, deoxyuridine monophosphate, for the thymidylate synthase (TS). It decreases the cellular content of uracil, reducing uracil misincorporation into DNA. Suppressing the dUTPase activity, …

University Of Nebraska-Lincoln Agricultural Research Division 121st Annual Report. July 1, 2006 To June 30, 2007., Institute Of Agriculture And Natural Resources

Agricultural Research Division: News and Annual Reports

Our Mission..... 4

Foreword..... 5

Research Highlights..... 6

Faculty Awards and Recognitions....14

Graduate Student Awards and Recognitions...17

Undergraduate Honors Student Research Program...22

Variety and Germplasm Releases....23

Patents.....24

Administration.....25

Administrative Personnel....25

Organizational Chart....26

Administrative Units....27

IANR Research Facilities....28

Faculty.....29

Agricultural/Natural Resources Units....30

Education and Human Sciences Departments...39

Off-Campus Research Centers....40

Interdisciplinary Activities....41

Visiting Scientists/Research Associates....42

Research Projects.....47

Agricultural/Natural Resources Units....47

Education and Human Sciences Departments...52

Off-Campus Research Centers....52

Interdisciplinary Activities....53

Publications.....55

Agricultural/Natural Resources Units....60

Education and Human Sciences Departments...82

Off-Campus Research Centers....85

Research Expenditures....88

Susceptibility Of Northern Corn Rootworm (Diabrotica Barberi) Populations To Cry3bb1 And Gpp34/Tpp35ab1 Proteins In Seedling And Diet Overlay Toxicity Assays, Adriano E. Pereira, Man P. Huynh, Kyle J. Paddock, Ryan W. Geisert, Veronica Calles Torrez, Janet J. Knodel, Daniel J. Moellenbeck, Jordan D. Reinders, Lance J. Meinke, Sharon K. Schneider, Thomas A. Coudron, Kent S. Shelby, Bruce E. Hibbard

Department of Entomology: Faculty Publications

The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae) is a major pest of maize in the United States Corn Belt. Recently, resistance to Bacillus thuringiensis (Bt) maize was reported in North Dakota and increased use of Bt maize hybrids could facilitate resistance evolution in other maizeproducing states. In this study, susceptibility to Bt proteins was evaluated in wild D. barberi populations from 8 fields collected in 5 different states (Minnesota, Missouri, Nebraska, Iowa, and North Dakota). Field populations were compared to a susceptible D. barberi colony in seedling and diet toxicity assays conducted with 3 …

Using Encrypted Genotypes And Phenotypes For Collaborative Genomic Analyses To Maintain Data Confidentiality, Tianjing Zhao, Fangyi Wang, Richard Mott, Jack Dekkers, Hao Cheng

Department of Animal Science: Faculty Publications

To adhere to and capitalize on the benefits of the FAIR (findable, accessible, interoperable, and reusable) principles in agricultural genome- to-phenome studies, it is crucial to address privacy and intellectual property issues that prevent sharing and reuse of data in research and industry. Direct sharing of genotype and phenotype data is often prohibited due to intellectual property and privacy concerns. Thus, there is a pressing need for encryption methods that obscure confidential aspects of the data, without affecting the outcomes of certain statistical analyses. A homomorphic encryption method for genotypes and phenotypes (HEGP) has been proposed for single-marker regression in …

Factors Affecting Forage Quality And The Subsequent Response In Production And Energy Metabolism In Lactating Jersey Cows, Kassidy Kate Buse

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Forage quality is the potential of a forage to produce a production response in animals. Because of how essential they are in dairy cattle rations, forages can have major influence on the behavior and production of dairy cattle. In experiment 1, the effect of forage fragility on the rumen environment and digestibility was evaluated. Increasing the inclusion of a less fragile forage led to greater rumen retention time, which resulted in lower rumen pH and greater NDF digestibility. Experiment 2 evaluated the effects of diets high in either corn silage or alfalfa haylage supplemented with either corn- or soy-based protein …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …

Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward …

Environmental Sustainability Of Small Wastewater Treatment Systems And Pathways For Improvement, Matthew James Thompson

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Wastewater treatment protects society and the environment by reducing pollutants generated from communities prior to discharge into local water bodies. Small water resource recovery facilities (WRRFs) represent the majority of centralized wastewater treatment systems, are resource intensive, and often lack resources needed to evaluate sustainability improvements. The goal of this dissertation was to identify primary sources and drivers of environmental impacts in small WRRFs and exploration of pathways to improve their environmental sustainability.

Case study data collected from small WRRFs in Nebraska was analyzed using environmental life cycle assessment (LCA) to quantify the environmental impact of facilities and existing opportunities. …

Room Temperature Two-Dimensional Electron Gas Scattering Time, Effective Mass, And Mobility Parameters In AlXGa1−XN/Gan Heterostructures (0.07 ≤ X ≤ 0.42), Sean Knight, Steffen Richter, Alexis Papamichail, Philipp Kühne, Nerijus Armakavicius, Shiqi Guo, Axel R. Persson, Vallery Stanishev, Viktor Rindert, Per O. Å. Persson, Plamen P. Paskov, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Al_xGa_1-xN/GaN high-electron-mobility transistor (HEMT) structures are key components in electronic devices operating at gigahertz or higher frequencies. In order to optimize such HEMT structures, understanding their electronic response at high frequencies and room temperature is required. Here, we present a study of the room temperature free charge carrier properties of the two-dimensional electron gas (2DEG) in HEMT structures with varying Al content in the Al_xGa_1-xN barrier layers between x = 0:07 and x = 0:42. We discuss and compare 2DEG sheet density, mobility, effective mass, sheet resistance, and scattering times, …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern US, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean monthly …

Relativistic Ultrafast Electron Diffraction At High Repetition Rates, K. M. Siddiqui, D. B. Durham, F. Cropp, F. Ji, S. Paiagua, C. Ophus, N. C. Andresen, L. Jin, J. Wu, S. Wang, X. Zhang, W. You, M. Murnane, Martin Centurion, X. Wang, D. S. Slaughter, R. A. Kaindl, P. Musumeci, A. M. Minor, D. Filippetto

Martin Centurion Publications

The ability to resolve the dynamics of matter on its native temporal and spatial scales constitutes a key challenge and convergent theme across chemistry, biology, and materials science. The last couple of decades have witnessed ultrafast electron diffraction (UED) emerge as one of the forefront techniques with the sensitivity to resolve atomic motions. Increasingly sophisticated UED instruments are being developed that are aimed at increasing the beam brightness in order to observe structural signatures, but so far they have been limited to low average current beams. Here, we present the technical design and capabilities of the HiRES (High Repetition-rate Electron …

Covid‑19‑Related Risk, Resilience, And Mental Health Among Mexican American Mothers Across The First Year Of The Pandemic, Amy L. Non, Elizabeth S. Clausing, Sandraluz Lara‑Cinisomo, Kimberly L. D’Anna Hernandez

Center for Brain, Biology, and Behavior: Faculty Publications

Background Latina mothers have been especially affected by the pandemic and historically exhibit high rates of depression and anxiety. However, few longitudinal studies have assessed the effect of the pandemic on this vulnerable population. We hypothesized that COVID-19-related stressors would associate with psychological distress among Latina mothers across the first year of the pandemic.

Methods We investigated COVID-19-related impact, stigma, and fears across two critical time points and changes in these measures in relation to changes in maternal anxiety and depression among mothers of Mexican descent living in Southern California (n=152). Surveys were administered within 5–16 weeks of …

Examination Of The Prevalence And Behaviors Of Pathogenic And Spoilage Bacteria At Multiple Stages In The Meat Processing Chain, Samuel C. Watson

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

As long as there is food, there will also be bacteria that are capable of compromising the quality and safety of food. This dissertation aims to study bacteria capable of spoiling meat, causing illness, or both. The literature review summarizes the understanding of several bacterial pathogens, current issues in combating those pathogens in the food supply, and how spoilage Pseudomonas behave in the meat supply. Chapter 2 explored the impact of organic acid treatments on the spoilage microbiome of raw beef during extended storage. While Pseudomonas and Yersinia were both present in significant levels, Lactococcus was determined to be the …

Value-Added Green Processing Of Tomato Waste To Obtain A Stable Free-Flowing Powder Lycopene Formulation Using Supercritical Fluid Technology, Purlen Sezer Okur, Ozan Ciftci

Department of Food Science and Technology: Faculty Publications

The tomato processing industry generates large amounts of waste, which creates disposal problems and negatively impacts the environment. Tomato waste contains lycopene, high-value health- and wellness-promoting bioactive, and it is a potential source of natural lycopene for food and nutraceutical applications. However, isolation of lycopene from its source requires toxic solvents, and lycopene is unstable during storage. The objective of this study was to extract lycopene from tomato waste and to convert it to a shelf-stable and easy-to-use free-flowing powder form using green extraction and particle formation processes based on supercritical carbon dioxide (SC-CO2) technology. Lycopene was extracted using SC-CO2 …

Integrative Transcriptomics And Proteomics Profiling Of Arabidopsis Thaliana Elucidates Novel Mechanisms Underlying Spaceflight Adaptation, Gbolaga O. Olanrewaju, Natasha J. Haveman, Michael J. Naldrett, Anna-Lisa Paul, Robert J. Ferl, Sarah E. Wyatt

Nebraska Center for Biotechnology: Faculty and Staff Publications

Spaceflight presents a unique environment with complex stressors, including microgravity and radiation, that can influence plant physiology at molecular levels. Combining transcriptomics and proteomics approaches, this research gives insights into the coordination of transcriptome and proteome in Arabidopsis’ molecular and physiological responses to Spaceflight environmental stress. Arabidopsis seedlings were germinated and grown in microgravity (μg) aboard the International Space Station (ISS) in NASA Biological Research in Canisters – Light Emitting Diode (BRIC LED) hardware, with the ground control established on Earth. At 10 days old, seedlings were frozen in RNA-later and returned to Earth. RNA-seq transcriptomics and TMTlabeled LC-MS/MS proteomic …

Organic Electronics In Biosensing: A Promising Frontier For Medical And Environmental Applications, Jyoti Bala Kaushal, Pratima Raut, Sanjay Kumar

Durham School of Architectural Engineering and Construction: Faculty Publications

The promising field of organic electronics has ushered in a new era of biosensing technology, thus offering a promising frontier for applications in both medical diagnostics and environmental monitoring. This review paper provides a comprehensive overview of organic electronics’ remarkable progress and potential in biosensing applications. It explores the multifaceted aspects of organic materials and devices, thereby highlighting their unique advantages, such as flexibility, biocompatibility, and low-cost fabrication. The paper delves into the diverse range of biosensors enabled by organic electronics, including electrochemical, optical, piezoelectric, and thermal sensors, thus showcasing their versatility in detecting biomolecules, pathogens, and environmental pollutants. Furthermore, …

A Bio-Economic Modeling Analysis Of Livestock Grazing On Rangelands Utilizing A Supplementary Grazing Resource, Jessica L. Windh

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation is a compilation of 5 chapters culminating in a bioeconomic model of the interactions between an integrated crop-livestock system and native rangeland grazing system. The model is developed using data from the eastern Sandhills of Nebraska, a location where cropping systems of Eastern Nebraska border one of the last intact grasslands remaining in North America.

The first chapter introduces the reader to the systems that will be modeled in the dissertation. In Chapter 2 we used a linear regression to model grassland biomass production as a function of climatic variables; we were unsuccessful in modeling season-long production in …

Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs ( miRNAs ) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex ( MC ) . Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 ( DCL1 ) and SERRATE ( SE ) and directly binds the stem- loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript ( pri-miRNAs ) with MC are reduced. …

Comparative Analysis Of Swine Antibody Responses Following Vaccination With Live-Attenuated And Killed African Swine Fever Virus Vaccines, Hung Q. Luong, Huong T. L. Lai, Lam Q. Truong, The N. Nguyen, Hanh D. Vu, Hoa T. Nguyen, Lan T. Nguyen, Trang H. Pham, David Scott Mcvey, Hiep Vu

Nebraska Center for Virology: Faculty Publications

African swine fever virus (ASFV) is circulating in many swine-producing countries, causing significant economic losses. It is observed that pigs experimentally vaccinated with a live-attenuated virus (LAV) but not a killed virus (KV) vaccine develop solid homologous protective immunity. The objective of this study was to comparatively analyze antibody profiles between pigs vaccinated with an LAV vaccine and those vaccinated with a KV vaccine to identify potential markers of vaccineinduced protection. Thirty ASFV seronegative pigs were divided into three groups: Group 1 received a single dose of an experimental LAV, Group 2 received two doses of an experimental KV vaccine, …

Reduced Grey Matter Volume In Adolescents With Conduct Disorder: A Region‑Of‑Interest Analysis Using Multivariate Generalized Linear Modeling, Ru Zhang, Robert James R. Blair, Karina S. Blair, Matthew Dobbertin, Jaimie Elowsky, Johannah Bashford‑Largo, Ahria J. Dominguez, Melissa Hatch, Sahil Bajaj

Center for Brain, Biology, and Behavior: Faculty Publications

Background

Conduct disorder (CD) involves a group of behavioral and emotional problems that usually begins during childhood or adolescence. Structural brain alterations have been observed in CD, including the amygdala, insula, ventrolateral and medial prefrontal cortex, anterior cingulate cortex, and fusiform gyrus. The current study developed a multivariate generalized linear model (GLM) to differentiate adolescents with CD from typically developing (TD) adolescents in terms of grey matter volume (GMV).

Methods

The whole‐brain structural MRI data were collected from 96 adolescents with CD (mean age = 16.188 ± 1.259 years; mean IQ = 104.292 ± 8.107 ; 63 males) and 90 …

A Multi-Organ Maize Metabolic Model Connects Temperature Stress With Energy Production And Reducing Power Generation, Niaz Bahar Chowdhury, Margaret Simons- Senftle, Berengere Decouard, Isabelle Quillere, Martine Rigault, Karuna Anna Sajeevan, Bibek Acharya, Ratul Chowdhury, Bertrand Hirel, Alia Dellagi, Costas Maranas, Rajib Saha

Department of Chemical and Biomolecular Engineering: Faculty Publications

Climate change has adversely affected maize productivity. Thereby, a holistic understanding of metabolic crosstalk among its organs is important to address this issue. Thus, we reconstructed the first multi-organ maize metabolicmodel, iZMA6517, and contextualized itwith heat and cold stress transcriptomics data using expression distributed reaction flux measurement (EXTREAM) algorithm. Furthermore, implementing metabolic bottleneck analysis on contextualized models revealed differences between these stresses. While both stresses had reducing power bottlenecks, heat stress had additional energy generation bottlenecks.We also performed thermodynamic driving force analysis, revealing thermodynamics-reducing power-energy generation axis dictating the nature of temperature stress responses. Thus, a temperaturetolerant maize ideotype can …

Intraspecific Variation Of Morphological Traits Backed Up With Molecular Evidence Votes For Re‑Appraisal Of Hitherto Distinguished Balaustium Species—A Case Study Of Balaustium Murorum (Acariformes: Parasitengona, Erythraeidae), Joanna Mąkol, Magdalena Felska

Harold W. Manter Laboratory of Parasitology: Library Materials

Molecular examination of representatives of Balaustium from several populations in SW Poland, performed using the sequence data from the mitochondrial cytochrome c oxidase subunit I, confirmed their common specific affiliation and identity with Balaustium murorum. The potential presence of distinct species in the studied material, preliminarily inferred from the discovery of clusters as a result of Principal Component Analysis exploring the metric data sets, was rejected due to the finding of only one haplotype, at intra- and inter-population sampling. An insight into meristic traits in larvae, focused on chaetotaxy of legs, revealed wider variation than hitherto recognized for the …

Soft Microreactors For The Deposition Of Microstructures And The Related Surface Chemistries Of Polymeric Materials, Jessica Wagner

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The precise control over small volumes of liquids is of great interest to various fields such as biotechnology, drug development, and diagnostics. Working at small scales reduces cost, time, and waste, which is why microfluidic lab-on-chip technologies have become popular in a wide range of industries and applications. Additionally, there are differences in properties such as mass transport and heat dissipation at the micron scale compared to in bulk. Microfluidic devices contain several interfaces to consider when preparing to fabricate devices. The substrate/device, substrate/solution, and solution/device interfaces are all of importance and must carefully be tuned depending on the desired …

Center For Grassland Studies, November 2023

Center for Grassland Studies: Newsletters

Contents

Experiential Learning for Grassland Conservation by David Wedin

Center for Grassland Studies Policy Advisory Committee

Director’s Column by David Wedin

New Woody Invasions Ecologist Brings Additional Capacity for Grassland Conservation by Dillon Fogarty

Twidwell Racks-up awards for his work in Grassland Conservation by Geitner Simmons

Platte Basin Timelapse premieres New Film on Nine-Mile Prairie by David Wedin

CRAWL: Update on the Network for Integrated Agricultural Resilience Research by Craig Allen

CGS Outreach by Jerry Volesky

Center for Grassland Studies Awards 19 Scholarships Totaling $17,500 for 2023-2024 by David Wedin

Award winners:

Joseph O. Young Fund = Makenna Anderson, Makennen …

Why Domain-Specific Science Knowledge Matters In Teacher Certification: Focusing On Evidence For Effective Science Teaching (Revised Version), Elizabeth B. Lewis, Lyrica L. Lucas, Amy Tankersley, Elizabeth Hasseler

Department of Teaching, Learning, and Teacher Education: Faculty Publications

The landscape of teacher preparation is complex. From a research perspective, how to prepare teachers presents as a multilevel, multivariable puzzle. For decades, federal and state policymakers, educational researchers, and administrators, along with teacher education institutions, school districts, and other stakeholders have tried to determine and measure the key malleable factors that result in effective teaching (NRC, 2010).

Periodically, state departments of education review secondary science teaching endorsement policy guidelines. As revisions occur, teacher educators in higher education and district administrators need to engage in a multidisciplinary discussion about:

1. the ways in which strong domain-specific science content knowledge contributes …

Motif-Cluster: A Spatial Clustering Package For Repetitive Motif Binding Patterns, Mengyuan Zhou

School of Computing: Dissertations, Theses, and Student Research

Previous efforts in using genome-wide analysis of transcription factor binding sites (TFBSs) have overlooked the importance of ranking potential significant regulatory regions, especially those with repetitive binding within a local region. Identifying these homogenous binding sites is critical because they have the potential to amplify the binding affinity and regulation activity of transcription factors, impacting gene expression and cellular functions. To address this issue, we developed an open-source tool Motif-Cluster that prioritizes and visualizes transcription factor regulatory regions by incorporating the idea of local motif clusters. Motif-Cluster can rank the significant transcription factor regulatory regions without the need for experimental …