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Biodiversity Of Insect Populations In The Springfield Bog Metropark In Summit County, Ohio: A Secondary Succession Site, Cynthia Perkovich

Department of Entomology: Distance Master of Science Projects

An initiative has been taken up by many environmental agencies for the conservation and restoration of endangered wetland habitats. These unique ecosystems are being depleted of resources and acreage due to industrialization and extensive anthropogenic activities. Bogs are a unique subgroup that are diminishing worldwide. The Springfield Metropark Bog is a newly established conservation area in Summit County, Ohio, in the United States. Insect populations were recorded over four seasons, starting in March 2015 and concluding in February 2016. Samples were taken from 3 different sites within the conserved habitat including field site, forested area, and a manufactured pond. Using …

Technique For: Determination Of Insect Prey Selection By Insectivorous Bats, Merrill Tawse

Department of Entomology: Distance Master of Science Projects

Utilizing Fecal Pellet Analysis to Determine Prey Selection by Insectivorous Bats: For my Graduate Project I examined culled exoskeleton parts from bat fecal pellets (guano) to determine identifications of the insects preyed upon by a group of bats to their order and often family levels. Culled insect parts were permanently fixed on microscope slides and viewed under a dissection microscope to determine identifications and to quantify the percentage composition that they represented in the bat’s diet. Comparisons were made of prey selected by the different bat species that were foraging at the same location and at the same time. Comparisons …

Economic Feasibility Of High Omega-3 Soybean Oil In Mariculture Diets: A Sustainable Replacement For Fish Oil, Subir Bairagi, Richard K. Perrin, Lilyan E. Fulginiti, Tom Clemente, Cory Hungate, G. Key

Department of Agricultural Economics: Faculty Publications

No abstract provided.

The Implications Of Climate Change For Nebraska: Summary Report Of Sector-Based Roundtable Discussions, Donald A. Wilhite, Kimberly Morrow

National Drought Mitigation Center: Faculty Publications

This report is the result of the work of many individuals that contributed to the success of the series of eight sector-based roundtable events that were held in September and October of 2015. First and foremost, we would like to thank the organizers and the organizations they represent for their leadership in bringing together a diverse set of key stakeholders to discuss the implications of climate change on their sector. It was such a pleasure to work with such a dedicated group of individuals. Second, we would like to thank the more than 350 people who participated in these discussions …

Land-Use Change Reduces Habitat Suitability For Supporting Managed Honey Bee Colonies In The Northern Great Plains, Clint R. V. Otto, Cali L. Roth, Benjamin L. Carlson, Matthew D. Smart

Department of Entomology: Faculty Publications

Human reliance on insect pollination services continues to increase even as pollinator populations exhibit global declines. Increased commodity crop prices and federal subsidies for biofuel crops, such as corn and soybeans, have contributed to rapid land-use change in the US Northern Great Plains (NGP), changes that may jeopardize habitat for honey bees in a part of the country that supports >40% of the US colony stock. We investigated changes in biofuel crop production and grassland land covers surrounding ~18,000 registered commercial apiaries in North and South Dakota from 2006 to 2014. We then developed habitat selection models to identify remotely …

Stress-Induced Changes In Abundance Differ Among Obligate And Facultative Endosymbionts Of The Soybean Aphid, Laramy S. Enders, Nicholas Miller

Department of Entomology: Faculty Publications

Bacterial endosymbionts can drive evolutionary novelty by conferring adaptive benefits under adverse environmental conditions. Among aphid species there is growing evidence that symbionts influence tolerance to various forms of stress. However, the extent to which stress inflicted on the aphid host has cascading effects on symbiont community dynamics remains poorly understood. Here we simultaneously quantified the effect of host-plant induced and xenobiotic stress on soybean aphid (Aphis glycines) fitness and relative abundance of its three bacterial symbionts. Exposure to soybean defensive stress (Rag1 gene) and a neurotoxic insecticide (thiamethoxam) substantially reduced aphid composite fitness (survival 9 reproduction) …

Reduced Stability And Intracellular Transport Of Dsrna Contribute To Poor Rnai Response In Lepidopteran Insects, Jayendra Nath Shukla, Megha Kalsi, Amit Sethi, Kenneth E. Narva, Elane Fishilevich, Satnam Singh, Kanakachari Mogilicherla, Subba Reddy Palli

Department of Entomology: Faculty Publications

RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We investigated the reasons for varying RNAi efficiency between lepidopteran and coleopteran cell lines and also between the Colorado potato beetle, Leptinotarsa decemlineata and tobacco budworm, Heliothis virescens. The dsRNA either injected or fed was degraded faster in H. virescens than in L. …

Evaluation Of Different Insecticide Formulations Against Lipaphis Erysimi (Hemiptera: Aphididae), A Pest On Oil Seed Crop, Camelina Sativa In Pakistan, Muhammad Arshad, Rashad Rasool Khan, Muhammad Irfan Ullah, Muhammad Afzal, Bamphitlhi Tiroesele, Irfan Mustafa, John E. Foster

Department of Entomology: Faculty Publications

False flax, German sesame and Siberian oilseed are the names used for oil seed crop, Camelina sativa L. Crantz of the family Brassicaceae. Camelina sativa is a flexible, summer annual oilseed crop that can be raised in different climatic and soil conditions. The objectives of this study were to test the efficacy of different insecticides against aphid, Lipaphis erysimi on Canadian and Australian Camelina sativa varieties. Insecticides were tested at their field recommended doses using knapsack sprayer. The data on adult mortality of Lipaphis erysimi were recorded after 24, 48, 72, 96 and 120 h. Acetamiprid and mospilon were found …

Effects Of Shade And Feeding Zilpaterol Hydrochloride To Finishing Steers On Performance, Carcass Quality, Heat Stress, Mobility, And Body Temperature, Bradley M. Boyd, Steven D. Shackelford, Kristin E. Hales Hales, Tami M. Brown- Brandl, Meredith L. Bremer, Matthew L. Spangler, Tommy L. Wheeler, David A. King, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study evaluated the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, mobility, and body temperature. No effect on body temperature, or performance was observed for shaded cattle versus cattle in open pens. Feeding ZH increased HCW, LM area and decreased yield grade. Zilpaterol hydrochloride increased respiration rate but did not significantly affect body temperature or mobility. Across all treatments mobility decreased with time, therefore, cattle were least mobile at the time of harvest.

Effect Of Dam Age On Offspring Productivity, Aline G. Da Silva, Jacqueline A. Musgrave, John Nollette Nollette, Andy Applegarth, Rick N. Funston

Nebraska Beef Cattle Reports

Records collected from 1997 to 2014 were analyzed to evaluate the effects of dam age on off spring productivity. Steer calves born from young mothers (2 and 3 yr old dams) had lighter carcasses with more carcasses grading Choice and upper 2/3 Choice. Th e calving performance and reproductive performance in the second breeding season for heifer calves born from first calving dams were the lowest compared with heifers born from multiparous dams. A quadratic effect of dam age on off spring performance was observed; as dam age increased, off spring performance increased, until dam age reached 7 to 8 …

Impact Of Supplementing Cattle With Omnigen- Af At The Receiving Or Finishing Phase On Beef Shelf- Life, Katherine I. Domenech, Michael D. Chao, Joe Buntyn Buntyn, Ty B. Schmidt, Chris R. Calkins

Nebraska Beef Cattle Reports

A patented proprietary product, OmniGen- AF, was evaluated to extend beef steak shelf- life at 8, 22 and 29 days of aging. Th e three treatment groups included a control group with no supplementation, OmniGen- AF supplementation for28 d aft er receiving, and supplementation for 210 d during finishing. Th e inclusion of OmniGen- AF had no effect on tenderness or visual discoloration under retail display conditions and minimal effects on fatty acid composition. Although color stability seemed to be unaffected by the supplementation, feeding OmniGen- AF throughout the entire feeding period tended to decrease oxidation. Supplementing cattle with a …

Comprehensive Comparative Genomic And Transcriptomic Analyses Of The Legume Genes Controlling The Nodulation Process, Zhenzhen Qiao, Lise Pingault, Mehrnoush Nourbakhsh-Rey, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is one of the most essential plant nutrients and one of the major factors limiting crop productivity. Having the goal to perform a more sustainable agriculture, there is a need to maximize biological nitrogen fixation, a feature of legumes. To enhance our understanding of the molecular mechanisms controlling the interaction between legumes and rhizobia, the symbiotic partner fixing and assimilating the atmospheric nitrogen for the plant, researchers took advantage of genetic and genomic resources developed across different legume models (e.g., Medicago truncatula, Lotus japonicus, Glycine max, and Phaseolus vulgaris) to identify key regulatory protein coding genes of the nodulation …

Comparison Of An Ultra-Low Volume (Ulv) Sprayer Against A Conventional Sprayer, For Foliar Fertiliser And Fungicide Applications In Turfgrass, Jason Connor Ferguson, Roch E. Gaussoin, John A. Eastin, Matthew D. Sousek, Greg R. Kruger

Department of Agronomy and Horticulture: Faculty Publications

Two field studies (I and II) at the University of Nebraska-Lincoln: John Seaton Anderson Turfgrass Research Facility near Mead, NE, USA, were conducted to determine if a new ultra-low volume (ULV) sprayer can apply foliar nutrient, growth regulator, and fungicide treatments, in a manner similar to that of a conventional sprayer. Treatments were applied over creeping bentgrass ‘L-93’ (Agrostis stolonifera L.) managed as a fairway at 561 l · ha⁻¹ and 47 l · ha⁻¹ with the conventional and ULV sprayer, respectfully. Data were collected for chlorophyll content with a chlorophyll meter, and for the normalised difference …

Aquaculture Depredation By Double-Crested Cormorants Breeding In Eastern North America, Elizabeth C. Craig, D. Tommy King, Jed P. Sparks, Paul D. Curtis

United States Department of Agriculture Wildlife Services: Staff Publications

The double-crested cormorant (Phalacrocorax auritus) has undergone population expansion throughout much of its historical range since the 1970s, resulting in increased pressure on foraging habitats including real and perceived competition with commercial and sport fisheries and impacts on the aquaculture industry. The specific objectives of this study were to determine the stable isotope ratios of birds wintering at aquaculture facilities and natural freshwater and marine habitats, and to determine what percent of birds at distinct breeding colonies wintered in each of these habitats. We exploited natural variation in isotopic ratios of carbon, nitrogen, and sulfur (from feathers) to …

Dedicated Herbaceous Biomass Feedstock Genetics And Development, W. F. Anderson, G. Sarath, S. Edme, M. D. Casler, R. B. Mitchell, C. M. Tobias, A. L. Hale, S. E. Sattler, J. E. Knoll

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Biofuels and bio-based products can be produced from a wide variety of herbaceous feedstocks. To supply enough biomass to meet the needs of a new bio-based economy, a suite of dedicated biomass species must be developed to accommodate a range of growing environments throughout the USA. Researchers from the United States Department of Agriculture’s Agricultural Research Service (USDA-ARS) and collaborators associated with the USDA Regional Biomass Research Centers have made major progress in understanding the genetics of switchgrass, sorghum, and other grass species and have begun to use this knowledge to develop new cultivars with high yields and appropriate traits …

Effect Of Interpersonal And Cognitive Stressors On Habituation And The Utility Of Heart Rate Variability To Measure Habituation, Denise M. Feda, James N. Roemmich

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Habituation is a decrease in responding to a repeated stimulus. Operant responding and salivation measure habituation in eating behaviour research. Stress may increase eating by acting as a distractor, yielding spontaneous recovery and prolonging responding for food. Our research tested differences in the ability of cognitive and inter- personal stressors to recover responding for food. We also tested heart rate variability (HRV) as a measure of habituation. Twenty women worked for portions of macaroni and cheese for 15 trials on three separate laboratory visits. Between the 12th and 13th trial, one of three different stressor types (speech, stroop and subtraction) …

Cougar Predation Rates And Prey Composition In The Pryor Mountains Of Wyoming And Montana, Linsey W. Blake, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding predator-prey dynamics is a fundamental principle of ecology and an ideal component for management decisions. Across North America, the impact of cougars (Puma concolor) on their prey varies regionally. To document the relationships between cougars, bighorn sheep (Ovis canadensis canadensis), mule deer (Odocoileus hemionus), and feral horses (Equus caballus) on the Bighorn Canyon National Recreation Area and the Pryor Mountains, we deployed GPS collars on 6 cougars (the total number residing on the study area), and visited their clusters to determine predation rates and foraging patterns. We examined the composition of …

Social Contexts Of Infant Feeding And Infant Feeding Decisions, Ellen J. Schafer, Natalie A. Williams, Siri Digney, Marion E. Hare, Sato Ashida

Department of Child, Youth, and Family Studies: Faculty Publications

Background: Infant feeding takes place within a network of social relationships. However, the social context in which infant feeding advice is received remains underresearched.

Objective: The objective of this study was to evaluate the social contexts of infant feeding by examining individual and relationship characteristics of mothers and network members associated with advice to exclusively breastfeed, exclusively formula feed, or use a combination of breast milk and formula.

Methods: Information about 287 network members was reported by 80 low-income mothers during a one-time survey. Characteristics of relationships associated with mothers receiving advice (exclusively breastfeed/formula feed, combination feed) from each network …

Learning The Language Of Nature: Young Children As Mathematical Thinkers, Carolyn P. Edwards, Courtney Boise

Department of Child, Youth, and Family Studies: Faculty Publications

Math Early On, funded by the Buffett Early Childhood Fund, involves a partnership between the University of Nebraska-Lincoln (UNL) and the Educares of Nebraska, part of a national network of highquality child care centers for low-income children from birth to age 5. Th e goal of the Math Early On project is to off er professional development opportunities that build on the past successful professional development efforts of UNL’s NebraskaMATH and its Primarily Math initiative. The Primarily Math curriculum for primary teachers was adapted and redesigned to create new experiences for the purpose of enriching preschool teachers’ mathematical knowledge for …

‘Let’S Work Together Towards Children’S Nutrition’: Building Bridge Between Child Care Providers And Parents For Promoting Child Health, Dipti A. Dev, Courtney Byrd-Williams, Samantha Ramsay, Brent A. Mcbride, Ashleigh L. Murriel, Chrisa Arcan, Anna M. Adachi-Mejia

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To investigate childcare providers’ perspectives for communicating with parents to promote children’s nutrition and health.

Design, Setting, and Participants: In-person, semistructured interviews. Licensed center-based child care. Eighteenfull- time childcareproviders caring for children2-5 years old, 67% caring for children who pay with federal subsidy.

Outcome Measures and Analysis: Participants were selected using maximum variation purposive sampling. Interviews were conducted until saturation was achieved. NVivo, thematic analysis used to code and identify themes and meta-themes regarding providers' perspectives for parent communication.

Results: Two meta-themes emerged from providers’ responses: barriers to communication, and strategies to communication. Barriers to communication include: parents are …

Nutrition Education Resources In North Carolina–Based Head Start Preschool Programs: Administrator And Teacher Perceptions Of Availability And Use, Sarah Lisson, L. Suzanne Goodell, Dipti A. Dev, Kristi Wilkerson,, Archana V. Hegde, Virginia C. Stage

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: The purpose of this study was to provide new insight into common barriers to the availability and use of nutrition education (NE) resources in Head Start preschool programs based on administrator and teacher perceptions.

Methods: In-depth, semistructured phone interviews (n = 63) were conducted with administrators (n = 31) and teachers (n = 32) from North Carolina–based Head Start programs. Interviews were audio-recorded and transcribed verbatim. Data were analyzed qualitatively using content analysis to identify common themes.

Results: Five emergent themes were identified within the areas of NE resource availability and use and barriers to NE resource availability and …

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …

Land Use In The Northern Great Plains Region Of The U.S. Influences The Survival And Productivity Of Honey Bee Colonies, Matthew D. Smart, Jeff S. Pettis, Ned Euliss, Marla S. Spivak

Department of Entomology: Faculty Publications

The Northern Great Plains region of the US annually hosts a large portion of commercially managed U.S. honey bee colonies each summer. Changing land use patterns over the last several decades have contributed to declines in the availability of bee forage across the region, and the future sustainability of the region to support honey bee colonies is unclear. We examined the influence of varying land use on the survivorship and productivity of honey bee colonies located in six apiaries within the Northern Great Plains state of North Dakota, an area of intensive agriculture and high density of beekeeping operations. Land …

Linking Measures Of Colony And Individual Honey Bee Health To Survival Among Apiaries Exposed To Varying Agricultural Land Use, Matthew D. Smart, Jeff Pettis, Nathan Rice, Zac Browning, Marla Spivak

Department of Entomology: Faculty Publications

We previously characterized and quantified the influence of land use on survival and productivity of colonies positioned in six apiaries and found that colonies in apiaries surrounded by more land in uncultivated forage experienced greater annual survival, and generally more honey production. Here, detailed metrics of honey bee health were assessed over three years in colonies positioned in the same six apiaries. The colonies were located in North Dakota during the summer months and were transported to California for almond pollination every winter. Our aim was to identify relationships among measures of colony and individual bee health that impacted and …

Incidence Of Spodoptera Litura (Lepidoptera: Noctuidae) And Its Feeding Potential On Various Citrus (Sapindales: Rutaceae) Cultivars In The Sargodha Region Of Pakistan, Muhammad Ullah, Muhammad Arshad, Muhammad Afzal, Samina Khalid, Muqadas Saleem, Irfan Mustafa, Yasir Iftikhar, Jaime Molina-Ochoa, John E. Foster

Department of Entomology: Faculty Publications

Spodoptera litura F. (Lepidoptera: Noctuidae) adversely affects important crops such as cotton, okra, tomato, potato, and pumpkin. For the first time, this species was observed in a citrus nursery in the Sargodha Region of Pakistan. The principal objectives of this study were to determine the effects of several citrus (Sapindales: Rutaceae) cultivars, including Citrus reticulata Blanco (Feutrell’s Early, Seedless Kinnow, and Fairchild mandarin orange) and Citrus paradisi Macfad. (grapefruit), on feeding by S. litura, and the effects of these plants on the growth of the insect. Based on performance of 3rd instars, Feutrell’s Early and Seedless Kinnow supported maximum …

Honey Bee Gut Microbiome Is Altered By In-Hive Pesticide Exposures, Madhavi L. Kakumanu, Alison M. Reeves, Troy D. Anderson, Richard R. Rodrigues, Mark A. Williams

Department of Entomology: Faculty Publications

Honey bees (Apismellifera) are the primary pollinators of major horticultural crops. Over the last few decades, a substantial decline in honey bees and their colonies have been reported. While a plethora of factors could contribute to the putative decline, pathogens, and pesticides are common concerns that draw attention. In addition to potential direct effects on honey bees, indirect pesticide effects could include alteration of essential gut microbial communities and symbionts that are important to honey bee health (e.g.,immunesystem). The primary objective of this study was to determine the microbiome associated with honey bees exposed to commonly used in-hive …

Effects Of Pesticide Treatments On Nutrient Levels In Worker Honey Bees (Apis Mellifera), Haley K. Feazel-Orr, Katelyn M. Catalfamo, Carlyle C. Brewster, Richard D. Fell, Troy D. Anderson, Breanna E. Traver

Department of Entomology: Faculty Publications

Honey bee colony loss continues to be an issue and no factor has been singled out as to the cause. In this study, we sought to determine whether two beekeeper-applied pesticide products, tau-fluvalinate and Fumagilin-B, and one agrochemical, chlorothalonil, impact the nutrient levels in honey bee workers in a natural colony environment. Treatments were performed in-hive and at three different periods (fall, spring, and summer) over the course of one year. Bees were sampled both at pre-treatment and two and four weeks post-treatment, weighed, and their protein and carbohydrate levels were determined using BCA and anthrone based biochemical assays, respectively. …

Tolerance Of Ks-4202 Soybean To The Attack Of Bemisia Tabaci Biotype B (Hemiptera: Aleyrodidae), Patrícia L. Cruz, Edson L.L. Baldin, Leysimar R.P. Guimarães, Luiz E.R. Pannuti, Giuseppina P.P. Lima, Tiffany Heng-Moss, Thomas E. Hunt

Department of Entomology: Faculty Publications

Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae) is considered one of the most important pests of soybean, Glycine max L. (Merrill) (Fabaceae), in Brazil and worldwide. Although chemical control still represents the principal strategy used to control this insect, less aggressive strategies such as the use of resistant genotypes stand out as potentially efficient alternatives for integrated pest management programs. This study aimed to evaluate the possible occurrence of tolerance to B. tabaci biotype B in the ‘KS-4202’ soybean genotype, which is already recognized as tolerant to Aphis glycines Matsumura (Hemiptera: Aphididae) in the United States. The ‘Conquista’ Brazilian cultivar …

Biology And Economics Of Recommendations For Insecticide-Based Management Of Soybean Aphid, Robert L. Koch, Bruce D. Potter, Phillip A. Glogoza, Erin W. Hodgson, Christian H. Krupke, John F. Tooker, Christina D. Difonzo, Andrew P. Michel, Kelley J. Tilmon, Travis J. Prochaska, Janet J. Knodel, Robert Wright, Thomas E. Hunt, Bryan Jensen, Adam J. Varenhorst, Brian P. Mccornack, Kelly A. Estes, Joseph L. Spencer

Department of Entomology: Faculty Publications

Soybean aphid, Aphis glycines Matsumura, remains the key insect pest of soybean, Glycine max (L.) Merrill, in the north-central United States. Management of this pest has relied primarily on scouting and application of foliar insecticides based on an economic threshold (ET) of 250 aphids per plant. This review explains why this ET remains valid for soybean aphid management, despite changes in crop value and input costs. In particular, we review how soybean aphid impacts soybean yield, the role of biology and economics in recommendations for soybean aphid management, and the short- and long-term consequences of inappropriately timed insecticide applications.

Host Utilization Of Tbe Endoparasitoid, Cotesia Glomerata L. (Hymenoptera: Braconidae) In Different Instars Of Pieris Brassicae L. (Lepidoptera: Pieridae), M. I. Ullah, Muhammad Arshad, S. Ali, Yasir Iftikhar, Jaime Molina-Ochoa, John E. Foster

Department of Entomology: Faculty Publications

Pieris brassicae L. (Lepidoptera: Pieridae) is a destructive pest of brassicaceous plants around the world. Biological control of this pest has attained much attention being environment friendly. Parasitism rate and growth of the endoparasitoid species, Cotesia giomerata L. (Hymenoptera: Braconidae) was studied on first (L I) and second (L2) larval instars of P. brassicae. Adult fresh mass and developmental time ofthe parasitoid was higher when it parasitized Ll than L2 of host larvae. Thus, host instars contributed significant variations to parasitoids adult mass accumulation during their development. Parasitized host caterpillars in L2 grew significantly larger than in L …