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Relationship Of Molecular Breeding Value For Beef Tenderness With Heifer Traits Through Weaning Of Their First Calf, Robert A. Cushman, Gary L. Bennett, Richard G. Tait, Anthony K. Mcneel, Eduardo Casas, Timothy P.L. Smith, Harvey C. Freetly Oct 2021

Relationship Of Molecular Breeding Value For Beef Tenderness With Heifer Traits Through Weaning Of Their First Calf, Robert A. Cushman, Gary L. Bennett, Richard G. Tait, Anthony K. Mcneel, Eduardo Casas, Timothy P.L. Smith, Harvey C. Freetly

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Polymorphisms in μ-calpain (CAPN1) that beneficially associate with beef tenderness are reported to antagonistically associate with calving day in beef heifers and post-partum interval to estrus in beef cows. We, therefore, hypothesized that a molecular breeding value for slice shear force, calculated based on CAPN1 and calpastatin (CAST) genotypes, would demonstrate an antagonistic relationship between genomically predicted slice shear force and ordinal calving date in replacement beef heifers. A secondary objective of this study was to evaluate the association of a polymorphism in diacylglycerol O-acyltransferase (DGAT1) with reproductive traits in beef heifers. One hundred eighty-seven MARC III heifers (¼ Angus, …


Validation Of High-Resolution Melting Assays For The Detection Of Virulent Strains Of Escherichia Coli O26 And O111 In Beef And Pork Enrichment Broths, Frank J. Velez, Joseph M. Bosilevac, Prashant Singh Oct 2021

Validation Of High-Resolution Melting Assays For The Detection Of Virulent Strains Of Escherichia Coli O26 And O111 In Beef And Pork Enrichment Broths, Frank J. Velez, Joseph M. Bosilevac, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Extensive validation of diagnostic assays using widely collected surveillance samples is critical for developing pathogen detection assays. The detection of potentially virulent E. coli strains is critical to the red meat industry. We previously developed two high-resolution melting (HRM) assays for detecting potentially virulent and avirulent E. coli O26 and O111 strains. Assays were validated using enriched beef (n = 36) and pork (n = 36) samples collected as part of a U.S. federal regulatory surveillance program. Data from this study showed more than 90% sensitivity and specificity for both the HRM assays, demonstrating suitability for the red meat industry …


Health And Nutrition Club, Ashley Mcrae, Maggie Osborne Oct 2021

Health And Nutrition Club, Ashley Mcrae, Maggie Osborne

Honors Program: Expanded Learning Clubs

These lesson plans outline a Health and Nutrition Club for kindergarten to second graders. The lessons aim at educating today's youth about their health through fun, interactive activities. Feel free to use the lesson plans exactly or to adapt the lessons to your own needs.


Gamma Sigma Delta, Nebraska Chapter Newsletter, Issue #60 Fall 2021 Oct 2021

Gamma Sigma Delta, Nebraska Chapter Newsletter, Issue #60 Fall 2021

Gamma Sigma Delta, Nebraska Chapter: Newsletters

President's message -- Paul C. Hay

2020 Nebraska Chapter Awards, The Honor Society of Agriculture, Gamma Sigma Delta: Merit, Teaching, Extension, Research, Outstanding Graduate Student, Outstanding Undergraduate Senior, Outstanding Undergraduate Student

New members inducted

Gamma Sigma Delta committees 2021

Nomination information and criteria for awards: Merit, Extension, Teaching, Research, Outstanding Graduate Student

Membership information and form

Annual dues notice

Outstanding Graduate Student Award Fund

In memoriam: Lisa Brown Jasa (October 11, 1956-September 28, 2020)

In memoriam: Frederick Paul Baxendale (April 10, 1949-September 30, 2021)

In memoriam: Daniel Eugene Bigbee, Sr. (April 1930-November 1, 2021)


Ott, Megan And Joycin Stonacek. "Physiology For Kids Club." After-School Club Lesson Plans. University Of Nebraska, 2021., Megan Ott, Joycin Stonacek Oct 2021

Ott, Megan And Joycin Stonacek. "Physiology For Kids Club." After-School Club Lesson Plans. University Of Nebraska, 2021., Megan Ott, Joycin Stonacek

Honors Program: Expanded Learning Clubs

No abstract provided.


Up And Atom, Emily Plotnik, Elizabeth Struwe Oct 2021

Up And Atom, Emily Plotnik, Elizabeth Struwe

Honors Program: Expanded Learning Clubs

"Up and Atom" is a hands-on and creative afterschool club geared towards fourth and fifth graders. Through conducting a variety of science experiments, the goal of this club is to inspire a creative and immersive perspective of the sciences for the younger generation.


Science, Sustainability, And Saving The World Club, Duncan Works, Sarah Brady Oct 2021

Science, Sustainability, And Saving The World Club, Duncan Works, Sarah Brady

Honors Program: Expanded Learning Clubs

After-school club lesson plans to educate grade school students on sustainable practices and inform them of the earth's changing climate.


Droughtscape, Fall 2021, National Drought Mitigation Center Oct 2021

Droughtscape, Fall 2021, National Drought Mitigation Center

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

DroughtScape, Fall 2021


Gsl Researcher (Fall 2021) Oct 2021

Gsl Researcher (Fall 2021)

GSL Researcher

GSL Researcher (Fall 2021)

Greetings

Students

GSL spotlight

Range and pasture update

Technical note

Ranch update

Highlighted research

GSL Open House agenda

Awards


Unl Emeriti And Retirees Association Newsletter, Fall 2021 Oct 2021

Unl Emeriti And Retirees Association Newsletter, Fall 2021

UNL Emeriti and Retirees Association: Newsletters and Other Materials

University of Nebraska-Lincoln Emeriti and Retirees Association Newsletter, Fall 2021


Water Current, Volume 53, No. 2, Fall 2021 Oct 2021

Water Current, Volume 53, No. 2, Fall 2021

Water Current Newsletter

Fall Harvest: Research, News and More from the Nebraska Water Center


Recent Updates On Ions And Nutrients Uptake By Halotolerant Freshwater And Marine Microalgae In Conditions Of High Salinity, Abdul Mannan Zafar, Muhammad Asad Javed, Ashraf Aly Hassan, Khalid Mehmood, Endalkachew Sahle-Demessie Oct 2021

Recent Updates On Ions And Nutrients Uptake By Halotolerant Freshwater And Marine Microalgae In Conditions Of High Salinity, Abdul Mannan Zafar, Muhammad Asad Javed, Ashraf Aly Hassan, Khalid Mehmood, Endalkachew Sahle-Demessie

Department of Civil and Environmental Engineering: Faculty Publications

Algae is an appropriate natural resource to augment the optimal use of undesired ions in water and wastewater. Increasing algal cells, the consumption of particular ions, including chloride, nitrate, phosphate, and ammonium, provides a suitable way to optimize water treatment processes. Different algal species have the capability to survive in extreme salinities by developing resistance against osmotic pressure in saline water. The current study reviews the effect of salinity on algal biomass production, algal growth rate, chlorides, nitrates, phosphates, chemical oxygen demand (COD), total nitrogen, total phosphorus, and ammonium ions. Mainly algae cultivated in freshwater, synthetic brackish water, seawater, and …


Variations, Validations, Degradations, And Noninvasive Determination Of Pregnancy Using Fecal Steroid Metabolites In Free-Ranging Pronghorn, Cole A. Bleke, Eric M. Gese, Susannah S. French Oct 2021

Variations, Validations, Degradations, And Noninvasive Determination Of Pregnancy Using Fecal Steroid Metabolites In Free-Ranging Pronghorn, Cole A. Bleke, Eric M. Gese, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Pregnancy status is a key parameter used to assess reproductive performance of a species as it represents a starting point for measuring vital rates. Vital rates allow managers to determine trends in populations such as neonate survival and recruitment; two important factors in ungulate population growth rates. Techniques to determine pregnancy have generally involved capture and restraint of the animal to obtain blood samples for determining serum hormone levels. Non-invasive pregnancy assessment, via feces, eliminates any hazards between handler and animal, as well as removes handling-induced physiological biases. Using noninvasive fecal sampling, we conducted hormone validations, investigated pregnancy rates, and …


Adaptation Of An Artificial Bait To An Automated Aerial Delivery System For Landscape-Scale Brown Treesnake Suppression, Rafael A. Garcia, Thomas W. Mcauliffe, Lorelie P. Bumanlag, Shane R. Siers, Bruce A. Kimball Oct 2021

Adaptation Of An Artificial Bait To An Automated Aerial Delivery System For Landscape-Scale Brown Treesnake Suppression, Rafael A. Garcia, Thomas W. Mcauliffe, Lorelie P. Bumanlag, Shane R. Siers, Bruce A. Kimball

United States Department of Agriculture Wildlife Services: Staff Publications

Dead neonatal mice are currently used as bait for delivery of toxin to invasive brown treesnakes (Boiga irregularis) on Guam; once deployed in the field the mice are highly attractive to the snakes but only for about four days. An artificial bait containing a mixture of fats mimicking those in skin of the mice is also highly attractive to the snakes and remains attractive 2–3 times longer. The artificial bait, however, costs more than the mice, and is more difficult to attach to the capsules of a novel aerial bait delivery system. This paper describes a reformulation of the bait …


Threading The Needle: How Humans Influence Predator–Prey Spatiotemporal Interactions In A Multiple-Predator System, Asia Murphy, Duane R. Diefenbach, Mark Ternent, Matt Lovallo, David Miller Oct 2021

Threading The Needle: How Humans Influence Predator–Prey Spatiotemporal Interactions In A Multiple-Predator System, Asia Murphy, Duane R. Diefenbach, Mark Ternent, Matt Lovallo, David Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Perceived predation risk and the resulting antipredator behaviour varies across space, time and predator identity. Communities with multiple predators that interact and differ in their use of space, time of activity and hunting mode create a complex landscape for prey to avoid predation. Anthropogenic presence and disturbance have the potential to shift interactions among predators and prey and the where and when encounters occur. We examined how white-tailed deer Odocoileus virginianus fawn spatiotemporal antipredator behaviour differed along an anthropogenic disturbance gradient that had black bears Ursus americanus, coyotes Canis latrans, bobcats Lynx rufus and humans present. We quantified (a) spatial …


Teaching Muscle Function And Physical Fitness: An Afterschool Program For Elementary Schools, Julianna Peters Oct 2021

Teaching Muscle Function And Physical Fitness: An Afterschool Program For Elementary Schools, Julianna Peters

Honors Program: Senior Projects (Public)

For this creative project, a nine-week club curriculum about muscle function and exercise was designed for upper elementary students (4th-6th grade), integrating science and physical education. This curriculum aims to introduce students to a basic understanding of muscle function and their body utilizing a scientific lens while also encouraging movement through the education of different muscle groups. It principally covers the three types of muscle with an emphasis on skeletal muscles. Students engage in experiments and activities focused on different aspects of muscle function and then do exercises focused on certain muscle groups. Additionally, it aims to …


Seed Coat Color Genetics And Genotype × Environment Effects In Yellow Beans Via Machine-Learning And Genome-Wide Association, Rie Sadohara, Yunfei Long, Paulo Izquierdo, Carlos Urrea, Daniel Morris, Karen Cichy Sep 2021

Seed Coat Color Genetics And Genotype × Environment Effects In Yellow Beans Via Machine-Learning And Genome-Wide Association, Rie Sadohara, Yunfei Long, Paulo Izquierdo, Carlos Urrea, Daniel Morris, Karen Cichy

Panhandle Research and Extension Center

Common bean (Phaseolus vulgaris L.) is consumed worldwide, with strong regional preferences for seed appearance characteristics. Colors of the seed coat, hilum ring, and corona are all important, along with susceptibility to postharvest darkening, which decreases seed value. This study aimed to characterize a collection of 295 yellow bean genotypes for seed appearance and postharvest darkening, evaluate genotype × environment (G × E) effects and map those traits via genome-wide association analysis. Yellow bean germplasm were grown for 2 yr in Michigan and Nebraska and seed were evaluated for L*a*b* color values, postharvest darkening, and hilum ring and corona …


Resistance Of Soybean Genotypes To Anticarsia Gemmatalis (Lepidoptera: Erebidae): Antixenosis And Antibiosis Characterization, S. Ongaratto, C. M. Silveira, M. C. Santos, J. E. R. Gorri, M. M.P. Sartori, Thomas E. Hunt, A. L. Lourenção, E. L.L. Baldin Sep 2021

Resistance Of Soybean Genotypes To Anticarsia Gemmatalis (Lepidoptera: Erebidae): Antixenosis And Antibiosis Characterization, S. Ongaratto, C. M. Silveira, M. C. Santos, J. E. R. Gorri, M. M.P. Sartori, Thomas E. Hunt, A. L. Lourenção, E. L.L. Baldin

Haskell Agricultural Laboratory (Northeast Research and Extension Center)

Injury by herbivores is a major biotic stress that limits soybean [Glycine max (L.) Merrill] crop production. Among the main soybean insect pests, Anticarsia gemmatalis Hübner is responsible for causing significant economic damage in soybean. The primary management strategy for this insect is chemical control and use of Bt transgenic soybean. Alternative strategies, such as host plant resistance, are considered an efficient and less-aggressive method, especially in association with other strategies as part of an integrated pest management (IPM) approach. In this study, we evaluated 30 soybean genotypes to verify antixenosis expression through oviposition, attractiveness, and food consumption tests. …


Characterization Of Tea (Camellia Sinensis) Granules For Quality Grading Using Computer Vision System, Md Towfiqur Rahman, Sabiha Ferdous, Mariya Sultana Jenin, Tanjina Rahman Mim, Masud Alam, Muhammad Rashed Al Mamun Sep 2021

Characterization Of Tea (Camellia Sinensis) Granules For Quality Grading Using Computer Vision System, Md Towfiqur Rahman, Sabiha Ferdous, Mariya Sultana Jenin, Tanjina Rahman Mim, Masud Alam, Muhammad Rashed Al Mamun

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Tea (Camellia sinensis) has been found as an important medicinal beverage for human which is consumed all over the world. Primarily, the majority of tea is being cultivated in Asia and Africa, however it is commercially produced by more than 60 countries. Though substantial amount is produced, its processing system is still underdeveloped which leads to decrease in export opportunity as well as low monetary value. Moreover, the traditional method of tea grading and sorting is laborious, inefficient, and costly which ultimately produces the low-quality heterogeneous products. Processing and grading of tea granules after drying is very important …


Multi-Agent Scavenging Patterns In Hawai‘I: A Forensic Archaeological And Skeletal Case Study, Jennifer F. Byrnes, William Belcher Sep 2021

Multi-Agent Scavenging Patterns In Hawai‘I: A Forensic Archaeological And Skeletal Case Study, Jennifer F. Byrnes, William Belcher

Department of Anthropology: Faculty Publications

Knowledge of the behavior of local fauna can aid forensic investigators in developing awareness of site formation processes. In Hawai‘i, little has been published on the effects of feral domestic pig (Sus scrofa) and feral domestic dog (Canis familiaris) scavenging and bone dispersal on field recovery and laboratory observations. In this Pacific tropical setting, the most consequential terrestrial taphonomic agents are pigs and dogs, both in terms of hard tissue modification and dispersal of remains across the landscape. In 2017, an archaeologist discovered the remains of an unidentified decedent on the island of Kauaʻi, State of Hawai‘i during a cultural …


Therapeutic Potential Of Garlic Chive-Derived Vesicle-Like Nanoparticles In Nlrp3 Inflammasome-Mediated Inflammatory Diseases, Baolong Liu, Xingzhi Li, Han Yu, Xuan Shi, You Zhou, Sophie Alvarez, Michael J. Naldrett, Stephen D. Kachman, Seung-Hyun Ro, Xinghui Sun, Soonkyu Chung, Lili Jing, Jiujiu Yu Sep 2021

Therapeutic Potential Of Garlic Chive-Derived Vesicle-Like Nanoparticles In Nlrp3 Inflammasome-Mediated Inflammatory Diseases, Baolong Liu, Xingzhi Li, Han Yu, Xuan Shi, You Zhou, Sophie Alvarez, Michael J. Naldrett, Stephen D. Kachman, Seung-Hyun Ro, Xinghui Sun, Soonkyu Chung, Lili Jing, Jiujiu Yu

Department of Nutrition and Health Sciences: Faculty Publications

Aberrant activation of the nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome drives the development of many complex inflammatory diseases, such as obesity, Alzheimer’s disease, and atherosclerosis. However, no medications specifically targeting the NLRP3 inflammasome have become clinically available. Therefore, we aim to identify new inhibitors of the NLRP3 inflammasome in this study. Methods: Vesicle-like nanoparticles (VLNs) were extracted from garlic chives and other Allium vegetables and their effects on the NLRP3 inflammasome were evaluated in primary macrophages. After garlic chive-derived VLNs (GC-VLNs) were found to exhibit potent anti-NLRP3 inflammasome activity in cell culture, …


Using Beet Pulp To Adapt Cattle To Finishing Diets Compared To Traditional Grain Adaptation With Alfalfa Hay, C. J. Schneider, Matt K. Luebbe, K. H. Jenkins, Stephanie A. Furman Furman, Galen E. Erickson, Terry J. Klopfenstein Sep 2021

Using Beet Pulp To Adapt Cattle To Finishing Diets Compared To Traditional Grain Adaptation With Alfalfa Hay, C. J. Schneider, Matt K. Luebbe, K. H. Jenkins, Stephanie A. Furman Furman, Galen E. Erickson, Terry J. Klopfenstein

Department of Animal Science: Faculty Publications

A study was conducted to compare grain adaptation programs using beet pulp (BP) to traditional grain adaptation with alfalfa hay (AH). Yearling crossbred steers (n = 232; BW = 326 ± 14.5 kg) were separated into 3 weight blocks, stratified by BW, and assigned randomly, within strata, to 18 feedlot pens, with 12 or 13 steers per pen. Treatments were imposed during grain adaptation (21 d) using 3 grain adaptation programs. Within each grain adaptation program, 4 step rations were fed for 3, 4, 7, and 7 d. Each program increased dry-rolled corn inclusion while roughage inclusion decreased. In the …


Center For Grassland Studies, September 2021 Sep 2021

Center For Grassland Studies, September 2021

Center for Grassland Studies: Newsletters

Contents

Nebraska Grazing Conference Recap by Daren Redfearn

Center for Grassland Studies Policy Advisory Committee

Director's Column by Walt Schacht (Interim Director)

Land Use and Property Taxes by Jordan Johnson

Congratulations August 2021 Graduates

Wildfires are Changing the Future of Nebraska’s Woodland-Grassland Complexes by Amanda Hefner

PGA WORKS Scholarship Recipients Announced by PGA Reach

Grazing Livestock Systems Internships Provide Diverse Experience by Bryan Reiling

Jim Choquette Receives Lifetime Achievement Award


Nebline, September/October 2021 Sep 2021

Nebline, September/October 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Youth Help Celebrate 150 Years of Lancaster County Fair

Farm to School Connections in Lancaster County

Recipe of the Month

Uses of Compost

Garden Guide: Things to Do This Month

GroBigRed Virtual Learning Series

Medically Important Spiders in Nebraska: The Black Widow and Brown Recluse

Herd Health Vaccinations

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

4-H State Public Speaking Top Results

Eight Lancaster 4-H Clubs Receive Governor’s Ag Excellence Awards

Sam’s Club Donates $1,000 to 4-H Council

Heart of 4-H Volunteer Award: Tim & Jenny Higgins

Heart of 4-H Volunteer Award: JJ & Amy Frink

Youth Science Day …


Advice For Students Of Color And First-Generation College Students: Successful Transitions From High School Into And Within College/University, Amanda Morales Sep 2021

Advice For Students Of Color And First-Generation College Students: Successful Transitions From High School Into And Within College/University, Amanda Morales

Department of Teaching, Learning, and Teacher Education: Faculty Publications

As you begin to read this pamphlet, you might ask, “Why should I listen to this person?” “What experiences has she had, or what expertise does she hold that makes her advice credible or worth my time and attention?” Well, given how busy we all are these days and knowing about all the advice out there about going to college, I see your point! However, I hope you find this piece a bit different from other resources. See, I approached the writing here in some ways as a consejo (a piece of advice) to my younger self. More specifically, I …


Scaup Depredation On Arkansas Baitfish And Sportfish Aquaculture, Stephen A. Clements, Brian S. Dorr, J. Brian Davis, Luke A. Roy, Carole R. Engle, Katie Hanson-Dorr, Anita M. Kelly Sep 2021

Scaup Depredation On Arkansas Baitfish And Sportfish Aquaculture, Stephen A. Clements, Brian S. Dorr, J. Brian Davis, Luke A. Roy, Carole R. Engle, Katie Hanson-Dorr, Anita M. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Lesser scaup (Aythya affinis) and greater scaup (A. marila), hereafter scaup, consume a variety of aquatic invertebrates, plants, and occasionally small fish. Scaup have foraged on commercial aquaculture farms in the southern United States for decades. However, the types, abundance, and rate of fish exploitation by scaup on baitfish and sportfish farms are not well documented. Thus, information is needed to understand how fish and other foods influence scaup use of aquatic resources, and any potential economic effects of depredation of fish. From November–March in winters 2016–2017 and 2017–2018, we conducted 1,458 pond surveys to …


Agricultural Carbon Markets: How Might They Work?, Drew Havens Sep 2021

Agricultural Carbon Markets: How Might They Work?, Drew Havens

Department of Agricultural Economics: Presentations, Working Papers, and Gray Literature

1) Introduction: a) Climate Change • b) Soil Sequestration and the Carbon Cycle • c) Increasing Soil Carbon Sequestration with Economics • d) Guiding Research Questions • e) Goals of this Report

2) What are agricultural carbon offset payment programs?

3) Are agricultural carbon markets effective?: • a) Voluntary vs Regulatory • b) Regional vs Global • c) Effectiveness of Agricultural Carbon Markets

4) Other Methods of Reducing Emissions: a) Carbon Tax • b) Cap and Trade • c) Summary

5) History of Programs That Provide Carbon Offset Payments to Farmers: a) The Chicago Climate Exchange • b) The Kyoto …


Associations Of Dietary Lipid-Soluble Micronutrients With Hepatic Steatosis Among Adults In The United States, Weiwen Chai, Sarah Eaton, Heather E. Rasmussen, Meng Hua Tao Aug 2021

Associations Of Dietary Lipid-Soluble Micronutrients With Hepatic Steatosis Among Adults In The United States, Weiwen Chai, Sarah Eaton, Heather E. Rasmussen, Meng Hua Tao

Department of Nutrition and Health Sciences: Faculty Publications

Lipid-soluble micronutrients may be beneficial to non-alcoholic fatty liver disease due to their important roles in metabolism and maintaining tissue functions. Utilizing 2017–2018 National Health and Nutrition Examination Survey, this study examined the potential overall and race/ethnicity-specific (black, Hispanic and white) associations of dietary lipid-soluble micronutrients (α-tocopherol, retinol, vitamin D, β-carotene and total carotenoids) with hepatic steatosis. The analysis included 4376 adults (1037 blacks, 981 Hispanics, 1549 whites) aged ≥20 years who completed the transient elastography examination with dietary data available. Odds ratios (OR) and 95% confidence intervals (95%CI) were estimated using logistic regressions. The age-adjusted prevalence of steatosis was …


Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang Aug 2021

Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat tenderness is an important quality trait critical to consumer acceptance, and determines satisfaction, repeat purchase and willingness-to-pay premium prices. Recent advances in tenderness research from a variety of perspectives are presented. Our understanding of molecular factors influencing tenderization are discussed in relation to glycolysis, calcium release, protease activation, apoptosis and heat shock proteins, the use of proteomic analysis for monitoring changes, proteomic biomarkers and oxidative/nitrosative stress. Each of these structural, metabolic and molecular determinants of meat tenderness are then discussed in greater detail in relation to animal variation, postmortem influences, and changes during cooking, with a focus on recent …


Skeletal Muscle Cell Growth Alters The Lipid Composition Of Extracellular Vesicles, Taylor R. Valentino, Blake D. Rule, C Brooks Mobley, Mariana Nikolova-Karakashian, Ivan Jose Vechetti Jr Aug 2021

Skeletal Muscle Cell Growth Alters The Lipid Composition Of Extracellular Vesicles, Taylor R. Valentino, Blake D. Rule, C Brooks Mobley, Mariana Nikolova-Karakashian, Ivan Jose Vechetti Jr

Department of Nutrition and Health Sciences: Faculty Publications

We sought to characterize the lipid profile of skeletal muscle cell-derived Extracellular Vesicles (EVs) to determine if a hypertrophic stimulus would affect the lipid composition of C2C12 myotube-derived EVs. Analyses included C2C12 murine myoblasts differentiated into myotubes and treated with Insulin-Like Growth Factor 1 (IGF-1) for 24 h to induce hypertrophic growth. EVs were isolated from cell culture media, quantified using Nanoparticle Tracking Analysis (NTA) and analyzed using Transmission Electron Microscopy (TEM). EVs were homogenized and lipids extracted for quantification by Mass Spectrometry followed by downstream lipid class enrichment and lipid chain analysis. IGF-1 treatment elicited an increase in CD63 …