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A Case-Control Study Of Hantavirus Pulmonary Syndrome During An Outbreak In The Southwestern United States, Paul S. Zeitz, Jay C. Butler, James E. Cheek, Michael C. Samuel, James E. Childs, Lee A. Shands, Richard E. Turner, Ronald E. Voorhees, John Sarisky, Pierre E. Rollin, Thomas G. Ksiazek, Louisa Chapman, Susan E. Reef, Kenneth K. Komatsu, Craig Dalton, John W. W. Krebs, Gary O. Maupin, Kenneth Gage, C. Mack Sewell, Robert F. Breiman, C. J. Peters

Zoonotics and Wildlife Disease: Publications

In May 1993, an outbreak of hantavirus pulmonary syndrome( HPS) occurred in the south-western United States. A case-control study determined risk factors for HPS. Seventeen case-patients were compared with 3 groups of controls: members of case-patient households( household controls), members of neighboring households( near controls), and members of randomly selected households ≥ 24 km away ( far controls). Investigators trapped more small rodents at case households than at near ( P = .03) or far control households( P = .02). After the number of small rodents was controlled for,case-patients were more likely than household controls to hand plow (odds ratio …

Guidelines For Working With Rodents Potentially Infected With Hantavirus, James N. Mills, Terry L. Yates, James E. Childs, Robert R. Parmenter, Thomas G. Ksiazek, Pierre E. Rollin, C.J. Peters

Zoonotics and Wildlife Disease: Publications

Because of the high morbidity and mortality associated with hantavirus pulmonary syndrome and the possibility of aerosol transmission of hantaviruses, persons handling known reservoir species in the field, laboratory, or classroom should take special precautions to minimize the risk of infection. We provide specific guidelines for personal safety while trapping, handling and releasing, transporting, sampling, and performing necropsy on potentially infected rodents or teaching field classes in areas occupied by reservoir species. Special consideration should be given to respiratory protection, choice and use of disinfectants, decontamination of instruments and traps, proper disposal of infectious wastes, and preservation and shipment of …

Host Age As A Determinant Of Naturally Acquired Immunity To Plasmodium Falciparum, J. Kevin Baird

United States Public Health Resources

The usual course of infection by Plasmodium falciparum among adults who lack a history of exposure to endemic malaria is fulminant. The infection in adults living with hyper- to holoendemic malaria is chronic and benign. Naturally acquired immunity to falciparum malaria is the basis of this difference. Confusion surrounds an essential question regarding this process: What is its rate of onset? Opinions vary because of disagreement over the relationships between exposure to infection, antigenic polymorphism and naturally acquired immunity. In this review, Kevin Baird discusses these relationships against a backdrop of host age as a determinant of naturally acquired immunity …

Isolation And Characterization Of A B-D-Glucuronidase-Producing Strain Of Escherichia Coli Serotype O157:H7 In The United States, Peggy S. Hayes, Kristina Blom, Peter Feng, Jay Lewis, Nancy A. Strockbine, Bala Swaminathan

United States Public Health Resources

A phenotypic variant of Escherichia coli serotype O157:H7 (G5101) was isolated from a patient with bloody diarrhea. Strain G5101 does not ferment sorbitol but is β-D-glucuronidase and urease positive. Serotyping and colony hybridization using a serotype-specific DNA probe confirmed that the isolate was O157:H7. G5101 produces Shiga-like toxins I and II and contains an eae gene that is highly conserved in the O157:H7 serotype. This strain would have been missed by laboratories that screen for the sorbitol-negative, β-D-glucuronidase- negative phenotype in isolating E. coli O157:H7 from clinical and food specimens.

Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore

Nebraska Center for Virology: Faculty Publications

In vitro neurovirological studies of viral infectivity or viral gene expression may be confounded by the mulHple neural cell types and/or fibrob last contamination present in early passage cultures prepared from dissociated human central nervous system (eNS) tissue. We have developed highly enriched astrocyte cultures for neurovirological study by culturing in a serum-free defined medium, 816, supplemented with basic fibroblast growth factor (FGF-2). Subculture in this medium selects against fibroblast proliferation and favors sustained proliferation of a highly enriched glial fibrillary acidic protein (GFAP)-positive cell population. These astrocytes support productive replication of cytomegalovirus (CMV) and transient expression of transfected CMVand …

G95-1259 Wildlife And Disease--Public Health Concerns, Dallas Virchow, Scott E. Hygnstrom, Wayne L. Kramer

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide describes human health risks and symptoms associated with prominent diseases of Nebraska's wildlife. Listed are precautions for minimizing exposure and preventing infection.

Zoonoses (zoe-uh'-no-sez') are diseases that can be transmitted from animals to humans. Wild animals and domestic animals share some disease-causing organisms and either group can transmit diseases to people. Transmission can occur directly through contact with tissues or body fluids of animals. Indirect transmission can occur through insects, ticks and mites that feed on infected animals.

Cc00-A Revised 1995 Resource: A Catalog Of Publications And Computer Programs

University of Nebraska-Lincoln Extension: Historical Materials

Extension Circular 00-A Revised 1995: Resource is a catalog of publications and computer programs.

How To Prune Trees, Peter J. Bedker, Joseph G. O’Brien, Manfred M. Mielke

Nebraska Forest Service: Publications

The objective of pruning is to produce strong, healthy, attractive plants. By understanding how, when and why to prune, and by following a few simple principles, this objective can be achieved.

Treatment Of Chloroquine-Resistant Plasmodium Vivax With Chloroquine And Primaquine Or Halofantrine, J. Kevin Baird, Hasan Basri, Budi Subianto, David J. Fryauff, Peter D. Mcelroy, Budhi Leksana, Thomas L. Richie, Sofyan Masbar, F. Stephen Wignall, Stehen L. Hoffman

United States Public Health Resources

Optimal therapy gor infection by chloroquine-resistant Plasmodium vivax has not been established. From 1992 to 1994 during three separate studies, 147 Javanese residents of Irian Jaya infected by P. vivax were treated with either chloroquine (2 5 mg of base/kg during 3 days or 10mg of base/kg in one dose) plus primaquine (1 0 mg/kg during 28 days or 2.5 mg/kg during 3 days)( n = 78), chloroquine plus placebo( n = 50), or halofantrine (24 mg base/kg in 12 h; n = 19). There was no difference in tolerance to or side effects of any of the regimens. Within …

Review Of Skeletal Biology In The Great Plains: Migration, Warfare, Health, And Subsistence, Edited By Douglas W. Owsley And Richard L. Jantz. Washington, D.C.: Smithsonian Institution Press, 1994. 415 Pages., Alan J. Osborn

Department of Anthropology: Faculty Publications

First paragraph:

The editors and contributors to this large, impressive volume present thirty-two chapters that deal with Great Plains skeletal biology. The goal of these diverse investigations was to derive critical information from human skeletal remains about past inhabitants of the Plains, including prehistoric and historic Indians, as well as Euro-Americans. These contributions are organized topically into five parts: (1) archaeology; (2) demography and paleopathology; (3) biological distance measures and skeletal morphology; (4) diet and subsistence strategies; and (5) warfare. The studies represent the collaborative efforts of archaeologists, physical anthropologists, ethnologists, ethnohistorians, and physical scientists. A major impetus for these …

Certain Canine Weakly Β-Hemolytic Intestinal Spirochetes Are Phenotypically And Genotypically Related To Spirochetes Associated With Human And Porcine Intestinal Spirochetosis, Gerald E. Duhamel, Nagaraja Muniappa, Michelle R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, A. R. Doster

School of Veterinary and Biomedical Sciences: Faculty Publications

Four canine weakly β-hemolytic intestinal spirochetes associated with intestinal spirochetosis (IS-associated WBHIS) were compared with IS-associated human and porcine WBHIS and the type species for Serpulina hyodysenteriae and S. innocens by using phenotypic and genotypic parameters. The IS-associated canine, human, and porcine WBHIS belonged to a phyletic group distinct from but related to previously described Serpulina type species.

Genetic Structure Of Midwestern Ascaris Suum Populations: A Comparison Of Isoenzyme And Rapd Markers, Steven A. Nadler, Rachel L. Lindquist, Thomas J. Near

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Isoenzyme and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetics of geographic variation among population samples of Ascaris suum from midwestern localities. Independent estimates of fixation indices (F_ST) based on isoenzyme and RAPD markers showed the same general patterns of differentiation and substantial statistical correlation (r=0.70). Of the total estimated gene diversity, 9.4% (isoenzyme) and 9.2% (RAPD) was distributed among infrapopulations. Geographic localities accounted for 7.8% (isoenzyme) and 6.2% (RAPD) of the total gene diversity. Only infrapopulations and localities, which indicates significant population subdivision among A. suum from farms within geographic regions. Departures from …

Microevolution And The Genetic Structure Of Parasite Populations, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The development of polymerase chain reaction-based methods for assessing the genotypes of small individual organisms will promote groundbreaking investigations of the genetic architecture of parasite populations. Both quantitative genetic models and general knowledge of parasite natural history are useful for making general predictions about the distribution of genetic variation over geographic space. However, designing experimental studies to assess relationships between specific life history variables and patterns of genetic structure in natural populations will be challenging. Traditional biochemical-genetic methods have already been used to study a limited number of parasite populations, and inferred patterns of genetic structure are distinctly different between …

Critical Comments: Parasitology Year 2000, Albert O. Bush, Janine N. Caira, Dennis J. Minchella, Steven A. Nadler, John R. Seed

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We predict that in order for parasitology to thrive by the year 2000 the various subdisciplines of evolution, ecology, biosystematics, and genetics must develop holistic approaches and use parasite models to answer basic biological questions. The students of tomorrow must work as part of a multidisciplinary team; and their questions and answers must be conceptually integrated into the broader biological framework of evolution and ecology.

Comparative Morphology Of The Scolices And Microtriches Among Five Species Of Tetrabothrius (Eucestoda: Tetrabothriidae), Eric P. Hoberg, D. E. Sims, P. H. Odense

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The structure of the scolex, neck, and adjacent strobila of 5 species of Tetrabothrius was evaluated using low to high magnification scanning electron miscroscopy. Species-specific patterns and morphotypes (spiniform, papilliform, and filiform) of microtriches were found on scolices and strobilar tegument of Tetrabothrius (Oriana) affinis, Tetrabothrius (Oriana) filiformis, and Tetrabothrius (Tetrabothrius) sp. The presence, distribution, and form of microtriches may not be correlated with the subgeneric divisions of Tetrabothrius or the host taxon. Unique patterns do not appear to be shared with other groups of Cestodes, particularly other tetraphyllideans. We suggest that the spiniform, filiform, and papilliform types of microtriches …

Libyostrongylus Dentatus N. Sp. (Nematoda: Trichostrongylidae) From Ostriches In North America, With Comments On The Genera Libyostrongylus And Paralibyostrongylus, Eric P. Hoberg, S. Lloyd, H. Omar

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Libyostrongylus dentatus sp. n. is described from ostriches on farms from North Carolina and Texas. Nematodes were recovered from the posterior proventriculus and under the koilon lining of the gizzard; the parasites occurred in mixed infections with Libyostrongylus douglassii. The species is distinguished from congeners by the presence of a prominent, dorsal, esophageal tooth; in males by the structure of the dorsal ray and spicules; and in females by small eggs (52-62 gm in length), a sublateral vulva situated at 93% of the body length from the anterior, and a strongly curled, digitate, tail with cuticular inflations at the …

Silver Staining For Elucidation Of The Synlophe In Trichostrongyle Nematodes, Alexander V. Khrustalev, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver-staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments current use of interference contrast for examination of the synlophe. Detailed studies of the configuration of the synlophe in entire specimens are possible with standard light microscopy for the first time.

Historical Biogeography And Modes Of Speciation Across High-Latitude Seas Of The Holarctic: Concepts For Host-Parasite Coevolution Among The Phocini (Phocidae) And Tetrabothriidae (Eucestoda), Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Species of Anophryocephalus are host-specific parasites of pinnipeds in the Holarctic. Phylogenetic analysis of 7 species postulates A. anophrys as the basal taxon and A. inuitorum as basal to A. skrjabini; A. arcticensis is basal to A. nunivakensis and A. eumetopii + A. ochotensis (single tree; consistency index = 74.4%; homoplasy slope ratio = 36.45%). Evaluation of host and geographic distributions postulates ringed seals of the Atlantic-Arctic as ancestral hosts, and the Arctic basin as a paraphyletic area with respect to the North Pacific. Cospeciation within this assemblage was dependent on intense isolation of small effective populations of definitive hosts …

Anophryocephalus Inuitorum Sp. Nov. And A. Arcticensis Sp. Nov. (Eucestoda: Tetrabothriidae) In Ringed Seals (Phoca Hispida Hispida) And Harp Seals (Phoca Groenlandica) From High-Latitude Seas Of Eastern Canada And The Arctic Basin, Eric P. Hoberg, Lena N. Measures

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Anophryocephalus inuitorum sp. nov. and A. arcticensis sp. nov. are described from ringed seals (Phoca hispida hispida) in the eastern Canadian Arctic; the latter species is also reported from harp seals (Phoca groenlandica) in the Gulf of St. Lawrence. Anophryocephalus inuitorum is most similar to A. skrjabini, but can be distinguished by fewer testes (14-27) and smaller dimensions of the strobila, neck (3.0-5.9 mm long), and cirrus sac (31-70 μm long), diameter of the genital atrium (44 -68 pm), and length of the male canal (23-42 μm long). Anophyrocephulus arcticensis resembles A. nunivakensis in the …

Cluster Analysis Of The Coprolites From Antelope House: Implications For Anasazi Diet And Cuisine, Mark Q. Sutton, Karl Reinhard

Karl Reinhard Publications

This paper reports on a cluster analysis of 155 coprolites from Antelope House, a prehistoric Anasazi site in Canyon de Chelly, Arizona. The analysis revealed three primary clusters; whole kernel maize, milled maize, and nonmaize, which we believe to represent seasonal- and preference-related cuisine. Protein residue analysis on a subsample of the specimens added depth to the analysis.

A New Pinworm, Didelphoxyuris Thylamisis N. Gen., N. Sp. (Nematoda: Oxyurida) From Thylamys Elegans (Waterhouse, 1839) (Marsupialia: Didelphidae) In Bolivia, Scott Lyell Gardner, Jean-Pierre Hugot

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Didelphoxyuris thylamisis n. gen., n. sp. is described from the caecum of Thylamys elegans (Waterhouse, 1839) (Marsupialia: Didelphidae) collected in the eastern region of the Andes of Bolivia. Didelphoxyuris thylamisis n. sp. differs from the only pinworm described from marsupials in the Neotropics (Neohilgertia venusti Navone, Suriano et Pujol, 1990) in having only three oesophageal teeth, non-operculated eggs, females that are didelphic, and males that possess no preanal papillae. Several other species of pinworms have been described from marsupials in Australia, but all are characterized by possessing a buccal capsule that is strongly cuticularized with inter-radial lamellae. These structures …

Caryospora Simplex (Apicomplexa: Eimeriidae) From A Captive Kaznakov's Viper (Vipera Kaznakovi), Patricia G. Wilber, John A. Hnida, Michael J. Richard, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Caryospora simplex is reported for the first time from the feces of a captive female Kaznakov’s viper (Vipera kaznakovi) in Albuquerque, New Mexico (USA). Coccidian meronts and gamonts were observed in the intestinal epithelial cells of another female Kaznakov’s viper that died in October 1993.

Caridean Shrimps Associated With The Slimy Sea Plume (Pseudopterogorigia Americana) In Midsummer At Guana Island, British Virgin Islands, West Indies, Stephen Spotte, Patricia M. Bubucis, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fifty-one slimy sea plumes (Pseudopterogorgia americana Gmelin, 1791) were sampled for caridean shrimps at Guana Island, British Virgin Islands, during one week in July 1992. Sam- pling depth ranged from 3-22 m. Nine species were collected: Hippolyte nicholsoni Chace, 1972; Latreutes sp.; Neopontonides chacei Heard, 1986; Perclimenes cf. patae Heard and Spotte, 1991; Periclimenes cf. pauper Holthuis, 1951; Periclimenes sp.; Pseudocoutierea antillensis Chace, 1972; Tozeuma cf. cornutum Milne Edwards, 1881; and Trachycaris rugosa (Bate, 1888). A total of 1,418 specimens (including fragments) was obtained. The number of shrimp species per gorgonian ranged from 1-5; one gorgonian harbored 156 shrimps. …

Disease And Coyotes In Texas, Danny B. Pence

Coyotes in the Southwest: A Compendium of Our Knowledge (Symposium Proceedings, 1995)

The coyote (Canis latrans) population in southern Texas has a recurring group of 3 common helminths and several peripheral species of lesser importance. Although recurrent group analyses have not been applied to other macro- or microparasite communities, there are certain infectious agents with high prevalences that could form recurrent groups, and that are potentially important in tams of impacting host population (i.e. coyote) abundance. While the current rabies epizootic involving coyotes in southern Texas is of public health concern, it probably will not have a major impact on the coyote population. Most likely, the net effect of canine …

Umingmakstrongylus Pallikuukensis Gen. Nov. Et Sp. Nov. (Nematoda: Protostrongylidae) From Muskoxen, Ovibos Moschafus, In The Central Canadian Arctic, With Comments On Biology And Biogeography, Eric P. Hoberg, Lydden Polley, A. Gunn, J. S. Nishi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Umingmakstrongylus pallikuukensis gen. nov. et sp. nov. is established for a protostrongylid nematode in muskoxen, Ovibos moschatus, from the Kitikmeot Region (central Arctic) of the Northwest Territories, Canada. It is distinguished from Cystocaulus and other Muelleriinae by characters that include the following: males: deeply incised, bilobed bursa, independent externodorsal rays, telamon composed of distal transverse plate, absence of falcate crurae, and spicules not distally split; females: absence of provagina; and first-stage larvae: presence of three cuticular folds on the tail. The great length of females (468 mm) and males (171 mm) is exceptional among the Protostrongylidae. Pathognomonic lesions include …

Host Specificity Of Rhabdochona Canadensis (Nematoda: Rhabdochonidae) In Nebraska, Michael A. Barger, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Intermediate and definitive host specificity of Rhabdochona canadensis in Nebraska were investigated. Mayfly nymphs Trichorythodes sp. and Caenis sp. were found to serve as experimental intermediate hosts. Development inside the nymphs required approximately 10 days, with the worms passing through two molts and then becoming encapsulated in the hemocoel as infective third-stage juveniles. Survey data revealed that only the red shiner Cyprinella lutrensis serves as definitive host for R. canadensis in nature. Laboratory infections of Notropis dorsalis, N. stramineus, and Fundulus zebrinus. all of which were uninfected in nature, were attempted to determine if observed specificity was due …

Second Intermediate Host-Specificity Of Haematoloechus Complexus And Haematoloechus Medioplexus (Digenea: Haematoloechidae), Scott D. Snyder, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Second intermediate host-specificity was examined for two species of the frog lung fluke genus Haematoloechus. Nine species of freshwater arthropods were exposed to cercariae of H. complexus and H. medioplexus. Metacercariae of H. complexus developed in all arthropod species used. Metacercariae of H. medioplexus developed only in anisopteran odonate naiads. This difference in host utilization may have epizootiological implications. The potential development of H. complexus in a greater number of arthropods than H. medioplexus may increase the chances of ingestion of H. complexus by an anuran host. The range of arthropods parasitized by H. complexus indicates that host-specificity …

Phylogenetic Systematic Analysis Of The Trichostrongylidae (Nematoda), With An Initial Assessment Of Coevolution And Biogeography, Eric P. Hoberg, J. Ralph Lichtenfels

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic analysis of the subfamilies of the Trichostrongylidae based on 22 morphological transformation series produced a single cladogram with a consistency index (CI) = 74.2%. Monophyly for the family was supported by the structure of the female tail and copulatory bursa. Two major clades are recognized: the Cooperiinae clade with the basal Cooperiinae and Libyostrongylinae + Trichostrongylinae, and the Graphidiinae clade with the basal Graphidiinae and Ostertagiinae + Haemonchinae. Dendrograms presented by Durette- Desset (1985) (CI = 56.1%) and Lichtenfels (1987), based on the key to the Trichostrongylidae by Gibbons and Khalil (1982) (CI = 59.0%), were found to be …

Presentation Of The President's Special Award For Distinguished Service, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of the presentation of the American Society of Parasitologists President's Special Award for Distinguished Service to Mary Louise Hanson Pritchard for 1994, by Daniel R. Brooks.

Wildlife Diseases And Humans, Robert G. Mclean

Prevention and Control of Wildlife Damage Handbook

General Precautions

Directly transmitted diseases: rabies, hantavirus, trichinosis, mosquito-borne encephalitis

Tick-borne diseases: Colorado tick tever, Rocky Mountain spotted fever (tick-borne typhus), Lyme disease, tularemia, relapsing fever

Personal protection

Flea-borne diseases: plague, murine, typhus, fever commensal rodent-borne diseases, rat-bite fever, leptospirosis (Weil’s disease), salmonellosis, rickettsialpox

Bird-borne diseases: histoplasmosis, ornithosis, salmonellosis