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The Adverse Associations Of Classrooms’ Indoor Air Quality And Thermal Comfort Conditions On Students’ Illness Related Absenteeism Between Heating And Non-Heating Seasons—A Pilot Study, Shihan Deng

Durham School of Architectural Engineering and Construction: Faculty Publications

(1) The association of the indoor environmental conditions in classrooms with illnessrelated absenteeism (IRA) was not well investigated. In addition, studying the association between heating and non-heating seasons were very limited; (2) To fill this knowledge gap, a research team collected various indoor air quality (IAQ) and thermal comfort conditions (TC) of 85 elementary classrooms in two school districts from the Midwestern United States throughout an academic year; in total, 255 classroom visits were performed. A negative binomial regression model was implied to associate the classroom’s IAQ and TC with IRA, separating for heating and non-heating seasons; (3) During non-heating …

Public Service Motivation, Megan Paul

Umbrella Summaries

What is public service motivation?

Public service motivation (PSM) is defined as “an individual’s predisposition to respond to motives grounded primarily or uniquely in public institutions” (Perry & Wise, 1990). In other words, it is about a person’s desire or interest to serve the public, often in the public sector, but also in the private, non-profit sector. It was developed in response to calls to revive interest in working in government, to restore the country’s faith in public institutions (Perry & Wise, 1990). The goal of the research was to explore whether people had such values and interests and, if …

What Questions Are On The Minds Of Stem Undergraduate Students And How Can They Be Addressed?, Clara L. Meaders, Michelle K. Smith, Timothy Boester, Anne Bracy, Brian A. Couch, Abby G. Drake, Saima Farooq, Bashir Khoda, Cynthia Kinsland, A. Kelly Lane, Sarah E. Lindahl, William H. Livingston, Ayesha Maliwal Bundy, Amber Mccormick, Anya I. Morozov, Jennifer L. Newell-Caito, Katharine J. Ruskin, Mark A. Sarvary, Marilyne Stains, Justin R. St. Juliana, Stephanie R. Thomas, Cindy Van Es, Erin L. Vinson, Maren N. Vitousek, Mackenzie R. Stetzer

School of Biological Sciences: Faculty Publications

Addressing common student questions in introductory STEM courses early in the term is one way that instructors can ensure that their students have all been presented with information about how to succeed in their courses. However, categorizing student questions and identifying evidence-based resources to address student questions takes time, and instructors may not be able to easily collect and respond to student questions at the beginning of every course. To help faculty effectively anticipate and respond to student questions, we 1) administered surveys in multiple STEM courses to identify common student questions, 2) conducted a qualitative analysis to determine categories …

Best Practices For Preschool Music Education: Supporting Music‑Making Throughout The Day, Jentry Stoneman Barrett, Rachel E. Schachter, Danni Gilbert, Mathew Fuerst

Department of Child, Youth, and Family Studies: Faculty Publications

Active engagement in music has numerous academic and social benefits for young children and music-making is included in many early childhood standards and preschool curricula. The purpose of this article is to provide quality resources for classroom teachers to use in providing music-making activities for young children, ages 3–5. Although teachers may use music in their classrooms, we provide resources and suggestions for more intentional and extended integration of music-making. Specifically, we identify best practices for preschool music education based on key standards and research as well as with common music pedagogies. We then turn to concrete examples of how …

Rapid Estimation Of Salmonella Enterica Contamination Level In Ground Beef – Application Of The Time-To-Positivity Method Using A Combination Of Molecular Detection And Direct Plating, Dayna M. Harhay, Margaret D. Weinroth, James L. Bono, Gregory P. Harhay, Joseph M. Bosilevac

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Little progress has been made in decreasing the incidence rate of salmonellosis in the US over the past decade. Mitigating the contribution of contaminated raw meat to the salmonellosis incidence rate requires rapid methods for quantifying Salmonella, so that highly contaminated products can be removed before entering the food chain. Here we evaluated the use of Time-to-Positivity (TTP) as a rapid, semi-quantitative approach for estimating Salmonella contamination levels in ground beef. Growth rates of 14 Salmonella strains (inoculated at log 1 to −2 CFU/g) were characterized in lean ground beef mTSB enrichments and time-to-detection was determined using culture and molecular …

Assessment Of Alternative Models For Genetic Analysis Of Worm And Tick Infestation In Nellore Cattle, T. L. Passafaro, F. B. Lopes, Thomas W. Murphy, B. D. Valente, R. C. Leite, G. J.M. Rosa, F. L.B. Toral

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Worms and ticks are important parasites in beef cattle, especially in tropical areas, causing significant economic and production losses. Understanding animal-to-animal variation on infestation for these parasites might guide genetic selection and improvement of management practices to attenuate its detrimental effects. Statistical models used to analyze such traits usually assume a Gaussian distribution for the observed data. However, this assumption is quite often inappropriate for counting data. Therefore, the objectives of this study were: 1) Estimate genetic parameters for worms and tick infestations in Nellore cattle, and 2) To compare the overall performance of six data analysis approaches for worm …

Nebline, February 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Staying active in winter

Nebraska Extension’s Physical Activity Resources

At-Home Workout: No Equipment Needed

Recipe of the Month

2021 Flower All-America Selections Winners

Garden Guide Things to Do This Month

ProHort Lawn & Landscape Update

Know Your Bugs Before You Treat: Bed vs. Bat

More Than Counting: Incorporating Math Into Daily Interactions With Preschoolers

Pesticide Applicator Trainings

Total Coliform Bacteria in Drinking Water

HEART OF 4-H Volunteer Award, Laura Cook

Meet 2020–2021 4-H Teen Council

4-H Club Gives Youth With Special Needs Opportunity to Show Lambs

Pick-A-Pig Club Accepting New Members

Companion Animal Webinar Series

4-H Rabbit Show, March 20 …

Protocol For Monitoring Aquatic Invertebrates Of Small Streams In The Heartland Inventory & Monitoring Network, Version 2.1, David E. Bowles, Michael H. Williams, Hope R. Dodd, Lloyd W. Morrison, Janice A. Hinsey, J. Tyler Cribbs, Gareth A. Rowell, Michael D. Debecker, Jennifer L. Haack-Gaynor, Jeffrey M. Williams

United States National Park Service: Publications

Executive Summary

The Heartland Inventory and Monitoring Network (HTLN) is a component of the National Park Service’s (NPS) strategy to improve park management through greater reliance on scientific information. The purposes of this program are to design and implement long-term ecological monitoring and provide information for park managers to evaluate the integrity of park ecosystems and better understand ecosystem processes. Concerns over declining surface water quality have led to the development of various monitoring approaches to assess stream water quality. Freshwater streams in network parks are threatened by numerous stressors, most of which originate outside park boundaries. Stream condition and …

Female Rats Display Higher Methamphetamine-Primed Reinstatement And C-Fos Immunoreactivity Than Male Rats, Steven T. Pittenger, Shinnyi Chou, Nathan J. Murawski, Scott T. Barrett, Olivia Loh, Juan F. Duque, Ming Li, Rick A. Bevins

Department of Psychology: Faculty Publications

Methamphetamine (meth) dependence is often characterized by persistent and chronic relapse (i.e., return to drug use). Previous work suggests females may be at greater risk to relapse. In this study, we extended this limited evidence and identified sex-dependent neural substrates related to meth-triggered reinstatement. Male and female Sprague-Dawley rats were implanted with indwelling jugular catheters. Half of the rats were then trained to self-administer meth (0.05 mg/kg/inf); the other half self-administered saline during 21 daily sessions (2 h). Rats were then given 12 extinction sessions. Twenty-four hours after the last extinction session, rats received reinstatement testing. Half of the rats …

Report From The Conference, ‘Identifying Obstacles To Applying Big Data In Agriculture’, Emma L. White, J. Alex Thomasson, Brent Auvermann, Newell R. Kitchen, Leland Sandy Pierson, Dana Porter, Craig Baillie, Hendrik Hamann, Gerrit Hoogenboom, Todd Janzen, Rajiv Khosla, James Lowenberg-Deboer, Matt Mcintosh, Seth Murray, Dave Osborn, Ashoo Shetty, Craig Stevenson, Joe Tevis, Fletcher Werner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Data-centric technology has not undergone widespread adoption in production agriculture but could address global needs for food security and farm profitability. Participants in the U.S. Department of Agriculture (USDA) National Institute for Food and Agriculture (NIFA) funded conference, “Identifying Obstacles to Applying Big Data in Agriculture,” held in Houston, TX, in August 2018, defined detailed scenarios in which on-farm decisions could benefit from the application of Big Data. The participants came from multiple academic fields, agricultural industries and government organizations and, in addition to defining the scenarios, they identified obstacles to implementing Big Data in these scenarios as well as …

Seedextractor: An Open-Source Gui For Seed Image Analysis, Feiyu Zhu, Puneet Paul, Waseem Hussain, Kyle Wallman, Balpreet K. Dhatt, Jaspreet Sandhu, Larissa Irvin, Gota Morota, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Accurate measurement of seed size parameters is essential for both breeding efforts aimed at enhancing yields and basic research focused on discovering genetic components that regulate seed size. To address this need, we have developed an open source graphical user interface (GUI) software, SeedExtractor that determines seed size and shape (including area, perimeter, length, width, circularity, and centroid), and seed color with capability to process a large number of images in a time-efficient manner. In this context, our application takes 2 s for analyzing an image, i.e., significantly less compared to the other tools. As this software is open-source, it …

Effects Of Mindfulness Meditation On Selective, Sustained Attention, Brain Neural Oscillations, And Short-Term Memory, Anamaria Guzman

Honors Program: Senior Projects (Public)

The following extended literature review and research proposal study started initially as a complete research proposal but, due to the challenges COVID-19 has brought, it has become a stand-alone piece of work without data collection. The goal is to synthesize a broad range of literature and previous research on mindfulness meditation and its effects on attention, memory, and brain activity and thus, offering a new perspective and a proposed research path on this subject. This proposed research study, besides previous studies, indicates that mindfulness meditation is expected to improve and enhance selective and sustained attention, which results in better attentional …

Anti-Nlrp3 Inflammasome Natural Compounds: An Update, Baolong Liu, Jiujiu Yu

Department of Nutrition and Health Sciences: Faculty Publications

The nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome is a multimeric protein complex that recognizes various danger or stress signals from pathogens, the host, and the environment, leading to activation of caspase-1 and inducing inflammatory responses. This pro-inflammatory protein complex plays critical roles in pathogenesis of a wide range of diseases including neurodegenerative diseases, autoinflammatory diseases, and metabolic disorders. Therefore, intensive efforts have been devoted to understanding its activation mechanisms and to searching for its specific inhibitors. Approximately forty natural compounds with anti-NLRP3 inflammasome properties have been identified. Here, we provide an update …

Nebraska Statewide Groundwater-Level Monitoring Report 2020, Aaron R. Young, Mark E. Burbach, Leslie M. Howard, Sue Olafsen Lackey, Robert Matthew Joeckel

Conservation and Survey Division: Faculty and Staff Publications

The term “groundwater” has come to be all but synonymous with Nebraska. Nearly three-quarters of the total volume of the High Plains Aquifer lies beneath the state. Groundwater maintains our streams, our ecosystems, our people, and our vitally important agricultural economy. Nebraska’s total groundwater resource is vast, yet it is also vulnerable to natural and anthropogenic changes, necessitating a long-term commitment to wise management through informed decision-making. Monitoring, studying, and reporting form the essential basis for such management and, ultimately, for meeting the myriad challenges presented by change.

Identification Of Anti-Inflammatory Vesicle-Like Nanoparticles In Honey, Xingyi Chen, Boalong Liu, Xingzhi Li, Thuy T. An, You Zhou, Gang Li, Judy Wu-Smart, Sophie Alvarez, Michael J. Naldrett, James Eudy, Gregory Kubik, Richard A. Wilson, Stephen D. Kachman, Juan Cui, Jiujiu Yu

Department of Nutrition and Health Sciences: Faculty Publications

Honey has been used as a nutrient, an ointment, and a medicine worldwide for many centuries. Modern research has demonstrated that honey has many medicinal properties, reflected in its anti-microbial, anti-oxidant, and anti-inflammatory bioactivities. Honey is composed of sugars, water and a myriad of minor components, including minerals, vitamins, proteins and polyphenols. Here, we report a new bioactive component‒vesicle-like nanoparticles‒in honey (H-VLNs). These HVLNs are membrane-bound nano-scale particles that contain lipids, proteins and small-sized RNAs. The presence of plant-originated plasma transmembrane proteins and plasma membrane-associated proteins suggests the potential vesicle-like nature of these particles. H-VLNs impede the formation and activation …

Longitudinal Assessment Of The Bovine Ocular Bacterial Community Dynamics In Calves, Alison Bartenslager, Nirosh D. Aluthge, John Dustin Loy, Matthew M. Hille, Matthew L. Spangler, Samodha C. Fernando

Department of Animal Science: Faculty Publications

Background: Infectious Bovine Keratoconjunctivitis (IBK), commonly known as pinkeye, is one of the most significant diseases of beef cattle. As such, IBK costs the US beef industry at least 150 million annually. However, strategies to prevent IBK are limited, with most cases resulting in treatment with antibiotics once the disease has developed. Longitudinal studies evaluating establishment of the ocular microbiota may identify critical risk periods for IBK outbreaks or changes in the microbiota that may predispose animals to IBK.

Results: In an attempt to characterize the establishment and colonization patterns of the bovine ocular microbiota, we conducted a longitudinal study …

Recharacterization Of Stenaspis Audinet-Serville, 1834 With A New Species From Mexico (Coleoptera: Cerambycidae: Cerambycinae: Trachyderini), Bryan K. Eya

Insecta Mundi

The genus Stenaspis Audinet-Serville, 1834 (Coleoptera: Cerambycidae: Cerambycinae: Trachy­derini) is recharacterized by providing additional morphological features to enhance the definition of this genus. Stenaspis plagiata Waterhouse, 1877 is transferred to Crioprosopus Audinet-Serville, 1834, new com­bination, and the latest key to species of Crioprosopus is modified. A key to the genera of Group III-Stenaspes with “abruptly separated anteocular space,” and a key to species of Stenaspis is provided. Color illustrations of the available species are included. Stenaspis lingafelteri Eya, new species, from Mexico is described.

A Review Of Open Research Data Policies And Practices In China, Lili Zhang, Robert R. Downs, Jianhui Li, Liangming Wen, Chengzan Li

Copyright, Fair Use, Scholarly Communication, etc.

This paper initially conducts a literature review and content analysis of the open research data policies in China. Next, a series of exemplars describe data practices to promote and enable the use of open research data, including open data practices in research programs, data repositories, data journals, and citizen science. Moreover, the top four driving forces are identified and analyzed along with their responsible guiding work. In addition, the “landscape of open research data ecology in China” is derived from the literature review and from observations of actual cases, where the interaction and mutual development of data policies, data programs, …

Generating Pennycress (Thlaspi Arvense) Seed Triacylglycerols And Acetyl-Triacylglycerols Containing Medium-Chain Fatty Acids, Maliheh Esfahanian, Tara J. Nazarenus, Meghan M. Freund, Gary Mcintosh, Winthrop B. Phippen, Mary E. Phippen, Timothy P. Durrett, Edgar B. Cahoon, John C. Sedbrook

Department of Biochemistry: Faculty Publications

Thlaspi arvense L. (pennycress) is a cold-tolerant Brassicaceae that produces large amounts of seeds rich in triacylglycerols and protein, making it an attractive target for domestication into an offseason oilseed cash cover crop. Pennycress is easily genetically transformed, enabling synthetic biology approaches to tailor oil properties for specific biofuel and industrial applications. To test the feasibility in pennycress of producing TAGs and acetyl-TAGs rich in medium-chain fatty acids (MCFAs; C6–C14) for industrial, biojet fuel and improved biodiesel applications, we generated transgenic lines with seed-specific expression of unique acyltransferase (LPAT and diacylglycerol acyltransferase) genes and thioesterase (FatB) genes isolated from Cuphea …

Union Commitment And Participation, Tara Myers, Megan Paul

Umbrella Summaries

What are union commitment and participation?

Union commitment refers to the extent to which employees are dedicated to their union (Cooper-Hakim & Viswesvaran, 2005; Gordon, Philpot, Burt, Thompson, & Spiller, 1980). Union participation is active involvement in the union such as attending meetings, serving on committees, holding office, campaigning, voting, assisting with contracts and negotiations, managing union funds, and filing grievances (Anderson, 1979). Many researchers consider union participation as part of union commitment (e.g., Gordon et al., 1980). Others have speculated that union participation is a consequence of union commitment (e.g., Parks, Gallagher, & Fullagar, 1995). Additional ways to conceptualize …

Truck Platooning Effects On Girder Bridges, Joshua Steelman, Jay A. Puckett, Daniel Linzell, Bowen Yang

Nebraska Department of Transportation: Research Reports

Truck platooning – digitally linking two or more trucks to travel in a closely spaced convoy – is increasingly used to save fuel, and reduce driver work and road congestion. Currently, the platoon load effects with several constant headways on bridges have been evaluated and compared to AASHTO design and legal loads. However, reliability assessment and a more rigorous investigation of headway spacing assumptions for truck platoons are lacking. This research provides a framework for determining how much a platoon permit load might be increased given strict control over the load characteristics and operational tactics. The present research evaluates the …

Programmatic Foci Of Women In Academic Leadership Positions At Historically Black Colleges And Universities: Intersectionality And Institutional Mission, Nina F. Schor

Journal of Women in Educational Leadership

The present studies compare, between Historically Black Colleges and Universities (HBCUs; n=102) and a non-HBCU cohort matched for location, religious and vocational mission, and student enrollment (comparator CUs; n=102), the programmatic foci of women in institutional leadership positions. They demonstrate that, at HBCUs, women are more prevalent in leadership roles with male-dominated foci (e.g., finance), and less prevalent in roles with female-dominated foci (e.g., public relations) than at comparator CUs (p < 0.01). A survey of academic leaders (n=1,053 invited; 111 viewed survey; 83 completed survey) at these institutions indicates that women leaders at HBCUs more frequently fill institutional programmatic gaps than their counterparts at comparator CUs (p < 0.001) or men in any academic setting (p < 0.005). Reasons may include the social purpose of HBCUs; the stereotype threat of the traditional “service” role of women and the unique intersectionality encountered by Black women faculty; and the importance of race over gender in homosociability at HBCUs. This suggests that emphasis on the socioeconomic mission and philosophy of higher education may enhance faculty recruitment diversification efforts in higher education. It also raises the question of whether seeing women in atypical leadership roles influences the career aspirations and attitudes towards women leaders of the students, both men and women, at HBCUs.

Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakash K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan

Department of Nutrition and Health Sciences: Faculty Publications

Zika virus (ZIKV) infection to a pregnant woman can be vertically transmitted to the fetus via the placenta leading to Congenital Zika syndrome. This is characterized by microcephaly, retinal defects, and intrauterine growth retardation. ZIKV induces placental trophoblast apoptosis leading to severe abnormalities in the growth and development of the fetus. However, the molecular mechanism behind ZIKV-induced apoptosis in placental trophoblasts remains unclear. We hypothesize that ZIKV infection induces endoplasmic reticulum (ER) stress in the trophoblasts, and sustained ER stress results in apoptosis. HTR-8 (HTR-8/SVneo), a human normal immortalized trophoblast cell and human choriocarcinoma-derived cell lines (JEG-3 and JAR) were …

Malaria In Southern Venezuela: The Hottest Hotspot In Latin America, Maria Eugenia Grillet, Jorge E. Moreno, Juan V. Hernández-Villena, Maria F. Vincenti-González, Oscar Noya, Adriana Tami, Alberto Paniz-Mondolfi, Martin Llewellyn, Rachel Lowe, Ananias A. Escalante, Jan E. Conn

Harold W. Manter Laboratory of Parasitology: Library Materials

Malaria elimination in Latin America is becoming an elusive goal. Malaria cases reached a historical ~1 million in 2017 and 2018, with Venezuela contributing 53% and 51% of those cases, respectively. Historically, malaria incidence in southern Venezuela has accounted for most of the country’s total number of cases. The efficient deployment of disease prevention measures and prediction of disease spread to new regions requires an in-depth understanding of spatial heterogeneity on malaria transmission dynamics. Herein, we characterized the spatial epidemiology of malaria in southern Venezuela from 2007 through 2017 and described the extent to which malaria distribution has changed country-wide …

Exercise Shifts Hypothetical Food Choices Toward Greater Amounts And More Immediate Consumption, Karsten Koehler, Safiya E. Beckford, Elise Thayer, Alexandra R. Martin, Julie B. Boron, Jeffrey R. Stevens

Department of Nutrition and Health Sciences: Faculty Publications

Although exercise modulates appetite regulation and food intake, it remains poorly understood how exercise impacts decision-making about food. The purpose of the present study was to assess the impact of an acute exercise bout on hypothetical choices related to the amount and timing of food intake. Forty-one healthy participants (22.0 ± 2.6 years; 23.7 ± 2.5 kg/m² , 56% female) completed 45 min of aerobic exercise and a resting control condition in randomized order. Food amount preferences and intertemporal food preferences (preference for immediate vs. delayed consumption) were assessed using electronic questionnaires with visual food cues. Compared to rest, …

Applications Of The Critical Power Model To Dynamic Constant External Resistance Exercise: A Brief Review Of The Critical Load Test, Haley C. Bergstrom, Taylor K. Dinyer, Pasquale J. Succi, Caleb C. Voskuil, Terry J. Housh

Department of Nutrition and Health Sciences: Faculty Publications

The study and application of the critical power (CP) concept has spanned many decades. The CP test provides estimates of two distinct parameters, CP and W0 , that describe aerobic and anaerobic metabolic capacities, respectively. Various mathematical models have been used to estimate the CP and W0 parameters across exercise modalities. Recently, the CP model has been applied to dynamic constant external resistance (DCER) exercises. The same hyperbolic relationship that has been established across various continuous, whole-body, dynamic movements has also been demonstrated for upper-, lower-, and whole-body DCER exercises. The asymptote of the load versus repetition relationship is defined …

Improving Opportunities For New Value Of Open Data: Assessing And Certifying Research Data Repositories, Robert R. Downs

Copyright, Fair Use, Scholarly Communication, etc.

Investments in research that produce scientific and scholarly data can be leveraged by enabling the resulting research data products and services to be used by broader communities and for new purposes, extending reuse beyond the initial users and purposes for which the data were originally collected. Submitting research data to a data repository offers opportunities for the data to be used in the future, providing ways for new benefits to be realized from data reuse. Improvements to data repositories that facilitate new uses of data increase the potential for data reuse and for gains in the value of open data …

Emotional Intelligence Training, Megan Paul

Umbrella Summaries

What is emotional intelligence training?

Emotional intelligence (EI) refers to abilities or tendencies related to recognizing, understanding, and managing emotions. Because of its connections to important work outcomes such as job satisfaction, organizational commitment, job performance, and turnover intentions (e.g., Joseph, Jin, Newman, & O’Boyle, 2015; Miao, Humphrey, & Qian, 2016), there has been great interest in developing EI via coaching and training, even though it has not traditionally been regarded as something that is amenable to change through training. EI is conceptualized, and measured, in three different ways (Ashkanasy & Daus, 2005). When it was first conceived, EI was …

Intervention Of Climate‐Smart Practices In Wheat Under Rice‐ Wheat Cropping System In Nepal, Jamma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Kjatri-Chhetri, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Besides a proper agronomic management followed by Nepalese farmers, wheat (Triticum aestivum L.) production has been severely affected by changing climate. There are many interventions, including climate‐smart practices, to cope with this situation and possibly enhance crop and soil productivity. Field experiments were set up in a randomized complete block design with six treatments (TRT) with four replications in three locations (LOC) during wheat‐growing seasons in Nepal from 2014 to 2016. Treatments included (i) Controlled Practice (CP), (ii) Improved Low (IL), (iii) Improved High (IH), (iv) Climate Smart Agriculture Low (CSAL), (v) Climate Smart Agriculture Medium (CSAM), and (vi) Climate …

Long Non-Coding Rna Meg3 Deficiency Impairs Glucose Homeostasis And Insulin Signaling By Inducing Cellular Senescence Of Hepatic Endothelium In Obesity, Xiao Cheng, Mohamed Sham Shihabudeen Haider Ali, Matthew Moran, Martonio Ponte Viana, Sarah L. Schlichte, Matthew C. Zimmerman, Oleh Khalimonchuk, Mark W. Feinberg, Xinghui Sun

Department of Biochemistry: Faculty Publications

Obesity-induced insulin resistance is a risk factor for diabetes and cardiovascular disease. However, the mechanisms underlying endothelial senescence in obesity, and how it impacts obesity-induced insulin resistance remain incompletely understood. In this study, transcriptome analysis revealed that the long non-coding RNA (lncRNA) Maternally expressed gene 3 (Meg3) is one of the top differentially expressed lncRNAs in the vascular endothelium in diet-induced obese mice. Meg3 knockdown induces cellular senescence of endothelial cells characterized by increased senescence-associated β–galactosidase activity, increased levels of endogenous superoxide, impaired mitochondrial structure and function, and impaired autophagy. Moreover, Meg3 knockdown causes cellular senescence of hepatic endothelium in …