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After Forty Years, Nebraska Weighs In On Assisting Suicide: Criminal Liability For Assisting Suicide In Nebraska After State V. Stubbendieck, Samuel S. Baue
After Forty Years, Nebraska Weighs In On Assisting Suicide: Criminal Liability For Assisting Suicide In Nebraska After State V. Stubbendieck, Samuel S. Baue
Nebraska Law Review
In State v. Stubbendieck,the Nebraska Supreme Court affirmed the first conviction for assisting suicidein state history,even though the Nebraska Legislature had criminalized assisting suicide more than forty years prior.The Nebraska Legislature made assisting suicide a Class IV felony in 1977 by enacting section 28-307 of the Nebraska Revised Statutes, which reads in pertinent part: “A person commits assisting suicide when, with intent to assist another person in committing suicide, he aids and abets him in committing or attempting to commit suicide.”In interpreting the statute for the first time, the Nebraska Supreme Court clarified the legal contours of assisting suicide …
The Litigation Landscape Of Fraternity And Sorority Hazing: Criminal And Civil Liability, Gregory S. Parks, Elizabeth Grindell
The Litigation Landscape Of Fraternity And Sorority Hazing: Criminal And Civil Liability, Gregory S. Parks, Elizabeth Grindell
Nebraska Law Review
Hazing—“the act of placing another person in a ridiculous, humiliating, or disconcerting position as part of an initiation process”—has caused injury and death. Some of the benefits asserted by those who participate in hazing are that the practice “creates deep and long-lasting bonds among those who endure it, instills the values of the group in new members, builds character, demonstrates commitment to the group, forges a connection with all members who had previously endured the experience, and inspires the respect of one’s peers.” Yet numerous lawsuits against individuals, fraternal organizations, and educational institutions have prompted legislatures to pass hazing laws …
Income Inequality And Opioid Prescribing Rates: Exploring Rural/Urban Differences In Pathways Via Residential Stability And Social Isolation, Tse-Chuan Yang, Seulki Kim, Carla Shoff
Income Inequality And Opioid Prescribing Rates: Exploring Rural/Urban Differences In Pathways Via Residential Stability And Social Isolation, Tse-Chuan Yang, Seulki Kim, Carla Shoff
Department of Sociology: Faculty Publications
While opioid prescribing rates have drawn researchers’ attention, little is known about the mechanisms through which income inequality affects opioid prescribing rates and even less focuses on whether there is a rural/urban difference in mediating pathways. Applying mediation analysis techniques to a unique ZIP code–level dataset from several sources maintained by the Centers for Medicare and Medicaid Services, we explicitly examine two mechanisms through residential stability and social isolation by rural/urban status and find that (1) income inequality is not directly related to opioid prescribing rates, but it exerts its influence on opioid prescribing via poor residential stability and elevated …
The Oa Diamond Journals Study, Part 2: Recommendations, Arianna Becerril, Lars Bjørnshauge, Jeroen Bosman, Jan Erik Frantsvåg, Bianca Kramer, Pierre-Carl Langlais, Vanessa Proudman, Claire Redhead, Didier Torny, Pierre Mounier
The Oa Diamond Journals Study, Part 2: Recommendations, Arianna Becerril, Lars Bjørnshauge, Jeroen Bosman, Jan Erik Frantsvåg, Bianca Kramer, Pierre-Carl Langlais, Vanessa Proudman, Claire Redhead, Didier Torny, Pierre Mounier
Copyright, Fair Use, Scholarly Communication, etc.
Science Europe and cOAlition S publish an in-depth report and recommendations arising from a study of community-driven Open Access journals across the world that are free for readers and authors, usually referred to as 'OA diamond journals'.
The study examines the areas that are critical for OA diamond journals, from legal structures and governance to technical capabilities, editorial processes, and funding models. The recommendations made in the report are designed to help research funding organisations, institutions, scholarly societies, and infrastructures sustainably strengthen OA diamond journals in the context of Open Science.
The study was commissioned by cOAlition S and funded …
Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor
Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor
United States Department of Agriculture Wildlife Services: Staff Publications
Beaver-related restoration is a process-based strategy that seeks to address wide-ranging ecological objectives by reestablishing dam building in degraded stream systems. Although the beaver-related restoration has broad appeal, especially in water-limited systems, its effectiveness is not yet well documented. In this article, we present a process-expectation framework that links beaver-related restoration tactics to commonly expected outcomes by identifying the set of process pathways that must occur to achieve those expected outcomes. We explore the contingency implicit within this framework using social and biophysical data from project and research sites. This analysis reveals that outcomes are often predicated on complex process …
Inhibition Of Zika Virus Replication By G-Quadruplex-Binding Ligands, Indian Institute Of Technology Indore, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak
Inhibition Of Zika Virus Replication By G-Quadruplex-Binding Ligands, Indian Institute Of Technology Indore, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak
Nebraska Center for Virology: Faculty Publications
Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent years, the presence of non-canonical G-quadruplex (GQ) secondary structures in viral genomes has ignited significant attention as potential targets for antiviral strategy. In this study, we identified several novel conserved potential GQ structures by analyzing published ZIKV genome sequences using an in-house algorithm. Biophysical and biochemical analysis of the RNA sequences containing these potential GQ sequences suggested the existence of …
Multi‐Country Investigation Of The Diversity And Associated Microorganisms Isolated From Tick Species From Domestic Animals, Wildlife And Vegetation In Selected African Countries, Emanuela Olivieri, Edward Kariuki, Anna Maria Floriano, Michele Castelli, Yohannes Mulatu Tafesse, Giulia Magoga, Bersissa Kumsa, Matteo Montagna, Davide Sassera
Multi‐Country Investigation Of The Diversity And Associated Microorganisms Isolated From Tick Species From Domestic Animals, Wildlife And Vegetation In Selected African Countries, Emanuela Olivieri, Edward Kariuki, Anna Maria Floriano, Michele Castelli, Yohannes Mulatu Tafesse, Giulia Magoga, Bersissa Kumsa, Matteo Montagna, Davide Sassera
Harold W. Manter Laboratory of Parasitology: Library Materials
In many areas of Africa, recent studies highlighted the great impact of ticks on animal and human health throughout the continent. On the other hand, very limited information on the bacterial endosymbionts of the African ticks and their pattern of co-infections with other bacteria are found in literature, notwithstanding their pivotal role in tick survival and vector efficiency. Thus, we investigated the distribution of selected pathogenic and symbiotic bacteria in hard ticks collected from wild, domestic animals and from vegetation in various ecological zones in Africa and their co-occurrence in the same tick host. Overall, 339 hard ticks were morphologically …
Technological Advances To Address Current Issues In Entomology: 2020 Student Debates, Lina Bernaola, Molly Darlington, Kadie Britt, Patricia Prade, Morgan Roth, Adrian Pekarcik, Michelle Boone, Dylan Ricke, Anh Tran, Joanie King, Kelly Carruthers, Morgan Thompson, John J. Ternest, Sarah E. Anderson, Scott W. Gula, Kayleigh C. Hauri, Jacob R. Pecenka, Sajjan Grover, Heena Puri, Surabhi Gupta Vakil
Technological Advances To Address Current Issues In Entomology: 2020 Student Debates, Lina Bernaola, Molly Darlington, Kadie Britt, Patricia Prade, Morgan Roth, Adrian Pekarcik, Michelle Boone, Dylan Ricke, Anh Tran, Joanie King, Kelly Carruthers, Morgan Thompson, John J. Ternest, Sarah E. Anderson, Scott W. Gula, Kayleigh C. Hauri, Jacob R. Pecenka, Sajjan Grover, Heena Puri, Surabhi Gupta Vakil
University of Nebraska State Museum: Entomology Papers
The 2020 Student Debates of the Entomological Society of America (ESA) were live-streamed during the Virtual Annual Meeting to debate current, prominent entomological issues of interest to members. The Student Debates Subcommittee of the National ESA Student Affairs Committee coordinated the student efforts throughout the year and hosted the live event. This year, four unbiased introductory speakers provided background for each debate topic while four multi-university teams were each assigned a debate topic under the theme ‘Technological Advances to Address Current Issues in Entomology’. The two debate topics selected were as follows: 1) What is the best taxonomic approach to …
Low Energy Availability With And Without A High-Protein Diet Suppresses Bone Formation And Increases Bone Resorption In Men: A Randomized Controlled Pilot Study, Chaise Murphy, Laura D. Bilek, Karsten Koehler
Low Energy Availability With And Without A High-Protein Diet Suppresses Bone Formation And Increases Bone Resorption In Men: A Randomized Controlled Pilot Study, Chaise Murphy, Laura D. Bilek, Karsten Koehler
Department of Nutrition and Health Sciences: Faculty Publications
Suppression of insulin-like growth factor 1 (IGF-1) and leptin secondary to low energy availability (LEA) may contribute to adverse effects on bone health. Whether a high-protein diet attenuates these effects has not been tested. Seven men completed three five-day conditions operationally defined as LEA (15 kcal kg fat-free mass (FFM)-1 day-1) with low protein (LEA-LP; 0.8 g protein·kg body weight (BW)-1), LEA with high protein (LEA-HP; 1.7 g protein·kg BW-1) and control (CON; 40 kcal·kg FFM-1·day-1, 1.7 g protein·kg BW-1). In all conditions, participants expended 15 kcal·kg …
Structural Basis For Clostridium Perfringens Enterotoxin Targeting Of Claudins At Tight Junctions In Mammalian Gut, Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Structural Basis For Clostridium Perfringens Enterotoxin Targeting Of Claudins At Tight Junctions In Mammalian Gut, Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Department of Biochemistry: Faculty Publications
The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F strains produce an enterotoxin (CpE) that causes the third most common foodborne illness in the United States. CpE induces gut breakdown by disrupting barriers at cell–cell contacts called tight junctions (TJs), which are formed and maintained by claudins. Targeted binding of CpE to specific claudins, encoded by its C-terminal domain (cCpE), loosens TJ barriers to trigger molecular leaks between cells. Cytotoxicity results from claudin-bound CpE complexes forming pores in cell membranes. In mammalian tissues, 24 claudins govern TJ barriers—but the basis for …
Factors Leading To Successful Island Rodent Eradications Following Initial Failure, Araceli Samaniego, Peter Kappes, Keith Broome, Steve Cranwell, Richard Griffiths, Grant Harper, Pete Mcclelland, Russell Palmer, Gerard Rocamora, Keith Springer, David Will, Shane Siers
Factors Leading To Successful Island Rodent Eradications Following Initial Failure, Araceli Samaniego, Peter Kappes, Keith Broome, Steve Cranwell, Richard Griffiths, Grant Harper, Pete Mcclelland, Russell Palmer, Gerard Rocamora, Keith Springer, David Will, Shane Siers
United States Department of Agriculture Wildlife Services: Staff Publications
Island rodent eradications are increasingly conducted to eliminate the negative impacts of invasive rodents. The success rate in the tropics has been lower than in temperate regions, triggering research and reviews. Environmental factors unique to the tropics (e.g., land crabs and year-round rodent breeding) have been associated with eradication failure. Operational factors have also been important, but these have not been comprehensively assessed. The environmental and operational factors using global cases where rodent eradication initially failed and subsequent attempts occurred were compared. It was determined whether operational factors explained the initial failures, whether operational improvements explained subsequent successes, and whether …
Mini-Review On Capacity-Building For Data-Driven Early Childhood Systems: The Consortium For Pre-Primary Data And Measurement In Sub-Saharan Africa, Abbie Raikes, Rebecca Sayre, Dawn Davis
Mini-Review On Capacity-Building For Data-Driven Early Childhood Systems: The Consortium For Pre-Primary Data And Measurement In Sub-Saharan Africa, Abbie Raikes, Rebecca Sayre, Dawn Davis
Department of Child, Youth, and Family Studies: Faculty Publications
Low- and middle-income countries (LMIC) are increasing investments in early childhood development programs, including early childhood education. As programs reach scale, there is increasing demand for evidence on impacts of investments. Little work to date has examined capacity required to effectively use data at scale in LMIC, including opportunities and barriers to integrating data into ongoing program implementation and tracking child development and quality of services at scale. Below, we outline the rationale and approach of the Consortium for Pre-primary Data and Measurement in Sub-Saharan Africa, focused on building capacity for data-driven decision-making in early childhood systems. Themes from the …
Gratitude, Stephanie Weddington, Megan Paul
Gratitude, Stephanie Weddington, Megan Paul
Umbrella Summaries
What is gratitude?
There are three ways of thinking about gratitude—as a state, a trait, or a tendency that can be improved through practice. A person can experience a brief state of gratitude in response to a specific event or another person’s kind actions (Emmons & McCullough, 2003). Gratitude is also considered a more enduring personality trait, disposition, or life orientation in which an individual has a general propensity to feel grateful for, notice, and appreciate the positives in day-to-day life (McCullough et al., 2002; Wood et al., 2010). Finally, individuals can enhance their tendency to feel grateful over time …
Epigraph Hemagglutinin Vaccine Induces Broad Cross-Reactive Immunity Against Swine H3 Influenza Virus, Brianna L. Bullard, Brigette N. Corder, Jennifer Debeauchamp, Adam Rubrum, Bette Korber, Richard J. Webby, Eric A. Weaver
Epigraph Hemagglutinin Vaccine Induces Broad Cross-Reactive Immunity Against Swine H3 Influenza Virus, Brianna L. Bullard, Brigette N. Corder, Jennifer Debeauchamp, Adam Rubrum, Bette Korber, Richard J. Webby, Eric A. Weaver
School of Biological Sciences: Faculty Publications
Influenza A virus infection in swine impacts the agricultural industry in addition to its zoonotic potential. Here, we utilize epigraph, a computational algorithm, to design a universal swine H3 influenza vaccine. The epigraph hemagglutinin proteins are delivered using an Adenovirus type 5 vector and are compared to a wild type hemagglutinin and the commercial inactivated vaccine, FluSure. In mice, epigraph vaccination leads to significant cross-reactive antibody and T-cell responses against a diverse panel of swH3 isolates. Epigraph vaccination also reduces weight loss and lung viral titers in mice after challenge with three divergent swH3 viruses. Vaccination studies in swine, the …
Identifying Receptors For Neuropeptides And Peptide Hormones: Challenges And Recent Progress, Md Shadman Ridwan Abid, Somayeh Mousavi, James W. Checco
Identifying Receptors For Neuropeptides And Peptide Hormones: Challenges And Recent Progress, Md Shadman Ridwan Abid, Somayeh Mousavi, James W. Checco
Department of Chemistry: Faculty Publications
Intercellular signaling events mediated by neuropeptides and peptide hormones represent important targets for both basic science and drug discovery. For many bioactive peptides, the protein receptors that transmit information across the receiving cell membrane are not known, severely limiting these signaling pathways as potential therapeutic targets. Identifying the receptor(s) for a given peptide of interest is complicated by several factors. Most notably, cell-cell signaling peptides are generated through dynamic biosynthetic pathways, can act on many different families of receptor proteins, and can participate in complex ligand-receptor interactions that extend beyond a simple one-to-one archetype. Here, we discuss recent methodological advances …
Determination Of Vadose Zone And Saturated Zone Nitrate Lag Times Using Long-Term Groundwater Monitoring Data And Statistical Machine Learning, Martin J. Wells, Troy E. Gilmore, Natalie Nelson, John K. Bohlke
Determination Of Vadose Zone And Saturated Zone Nitrate Lag Times Using Long-Term Groundwater Monitoring Data And Statistical Machine Learning, Martin J. Wells, Troy E. Gilmore, Natalie Nelson, John K. Bohlke
Department of Agricultural and Biological Systems Engineering: Faculty Publications
In this study, we explored the use of statistical machine learning and long-term groundwater nitrate monitoring data to estimate vadose zone and saturated zone lag times in an irrigated alluvial agricultural setting. Unlike most previous statistical machine learning studies that sought to predict groundwater nitrate concentrations within aquifers, the focus of this study was to leverage available groundwater nitrate concentrations and other environmental variables to determine mean regional vertical velocities (transport rates) of water and solutes in the vadose zone and saturated zone (3.50 and 3.75 m yr−1 , respectively). The statistical machine learning results are consistent with two primary …
Pre-Training Interventions, Megan Paul
Pre-Training Interventions, Megan Paul
Umbrella Summaries
What are pre-training interventions?
Pre-training interventions refer to strategies that are implemented prior to training, for the purpose of enhancing training outcomes. Thus far, the primary strategies that have been most frequently investigated include (a) attentional advice, (b) meta-cognitive strategies, (c) advance organizers, (d) goal orientation, and (e) preparatory information (Cannon-Bowers et al., 1998; Mesmer-Magnus & Viswesvaran, 2010).
Attentional advice includes guidance to orient the learner to what they will learn. Examples include going over the main learning objectives or highlighting essential aspects of the training and how they relate to the job.
Meta-cognitive strategies include approaches for learners to …
Role Of Nadþ In Regulating Cellular And Metabolic Signaling Pathways, Sara Amjad, Sabah Nisar, Ajaz A. Bhat, Ab Rauf Shah, Michael P. Frenneaux, Khalid Fakhro, Mohammad Haris, Ravinder Reddy, Zoltan Patay, Joseph Baur, Puneet Bagga
Role Of Nadþ In Regulating Cellular And Metabolic Signaling Pathways, Sara Amjad, Sabah Nisar, Ajaz A. Bhat, Ab Rauf Shah, Michael P. Frenneaux, Khalid Fakhro, Mohammad Haris, Ravinder Reddy, Zoltan Patay, Joseph Baur, Puneet Bagga
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Background: Nicotinamide adenine dinucleotide (NAD+), a critical coenzyme present in every living cell, is involved in a myriad of metabolic processes associated with cellular bioenergetics. For this reason, NAD+ is often studied in the context of aging, cancer, and neurodegenerative and metabolic disorders.
Scope of review: Cellular NAD+ depletion is associated with compromised adaptive cellular stress responses, impaired neuronal plasticity, impaired DNA repair, and cellular senescence. Increasing evidence has shown the efficacy of boosting NAD+ levels using NAD+ precursors in various diseases. This review provides a comprehensive understanding into the role of NAD+ …
Testing The Use Of A Social Networking App For American Indians Recovering From Addiction, Nicholas Guenzel, Dennis Mcchargue, Hongying Dai
Testing The Use Of A Social Networking App For American Indians Recovering From Addiction, Nicholas Guenzel, Dennis Mcchargue, Hongying Dai
Department of Psychology: Faculty Publications
Objectives: American Indians (AIs) have higher rates of addiction than most other groups. Social networking mobile apps are growing in popularity but their use has not been studied among AIs specifically. Methods: This paper describes a pilot program in which 27 AIs recovering from addiction were given access to a mobile app to support addiction recovery (Sober Grid) for up to six months. They completed a technology acceptability survey, monthly surveys of cravings, social connectedness, and quality of life, and a follow-up survey. Their use of the app was also tracked. Findings: We found that individuals in the sample often …
Generation Of A Biobank From Two Adult Thoroughbred Stallions For The Functional Annotation Of Animal Genomes Initiative, Callum G. Donnelly, Rebecca R. Bellone, Erin N. Hales, Annee Nguyen, Scott A. Katzman, Ghislaine A. Dujovne, Kelly E. Knickelbein, Felipe Avila, Ted S. Kalbfleisch, Elena Giulotto, Nicole B. Kingsley, Jocelyn Tanaka, Elizabeth Esdaile, Sichong Peng, Anna Dahlgren, Anna Fuller, Michael J. Mienaltowski, Terje Raudsepp, Verena K. Affolter, Jessica L. Petersen, Carrie J. Finno
Generation Of A Biobank From Two Adult Thoroughbred Stallions For The Functional Annotation Of Animal Genomes Initiative, Callum G. Donnelly, Rebecca R. Bellone, Erin N. Hales, Annee Nguyen, Scott A. Katzman, Ghislaine A. Dujovne, Kelly E. Knickelbein, Felipe Avila, Ted S. Kalbfleisch, Elena Giulotto, Nicole B. Kingsley, Jocelyn Tanaka, Elizabeth Esdaile, Sichong Peng, Anna Dahlgren, Anna Fuller, Michael J. Mienaltowski, Terje Raudsepp, Verena K. Affolter, Jessica L. Petersen, Carrie J. Finno
Department of Animal Science: Faculty Publications
Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic …
Immunomodulatory Role Of Urolithin A On Metabolic Diseases, Ashley Mulcahy Toney, Darius Fox, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung
Immunomodulatory Role Of Urolithin A On Metabolic Diseases, Ashley Mulcahy Toney, Darius Fox, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung
Department of Nutrition and Health Sciences: Faculty Publications
Abstract: Urolithin A (UroA) is a gut metabolite produced from ellagic acid-containing foods such as pomegranates, berries, and walnuts. UroA is of growing interest due to its therapeutic potential for various metabolic diseases based on immunomodulatory properties. Recent advances in UroA research suggest that UroA administration attenuates inflammation in various tissues, including the brain, adipose, heart, and liver tissues, leading to the potential delay or prevention of the onset of Alzheimer’s disease, type 2 diabetes mellitus, and non-alcoholic fatty liver disease. In this review, we focus on recent updates of the anti-inflammatory function of UroA and summarize the potential mechanisms …
Sleep, Megan Paul
Sleep, Megan Paul
Umbrella Summaries
What is sleep?
In the context of the workplace, investigations into sleep tend to focus on two main aspects: sleep quantity and sleep quality. Though sleep is occasionally measured using objective measures that assess physiological indicators, subjective self-report measures are most often used (Litwiller et al., 2017). There are dozens of measures for assessing sleep quality or quantity, some of which are single items and others that include multiple items. A more commonly used multidimensional measure is the 19-item Pittsburgh Sleep Quality Index, which assesses factors like sleep duration, sleep disturbance, and sleep efficiency, among others (Buysse et al., 1989). …
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Food for Health: Publications
Zygnematophyceae green algae (ZGA) have been shown to be the closest relatives of land plants. Three nuclear genomes (Spirogloea muscicola, Mesotaenium endlicherianum, and Penium margaritaceum) of ZGA have been recently published, and more genomes are underway. Here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating −) and found distinct cell sizes and other morphological differences. The molecular identities of the two strains were further investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a were surprisingly much more similar to Z. cylindricum (SAG 698-2) than to SAG 698-1b. …
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Food for Health: Publications
Zygnematophyceae green algae (ZGA) have been shown to be the closest relatives of land plants. Three nuclear genomes (Spirogloea muscicola, Mesotaenium endlicherianum, and Penium margaritaceum) of ZGA have been recently published, and more genomes are underway. Here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating −) and found distinct cell sizes and other morphological differences. The molecular identities of the two strains were further investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a were surprisingly much more similar to Z. cylindricum (SAG 698-2) than to SAG 698-1b. …
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Characterization Of Two Zygnema Strains (Zygnema Circumcarinatum Sag 698-1a And Sag 698-1b) And A Rapid Method To Estimate Nuclear Genome Size Of Zygnematophycean Green Algae, Xuehuan Feng, Andreas Holzinger, Charlotte Permann, Dirk Anderson, Yanbin Yin
Food for Health: Publications
Zygnematophyceae green algae (ZGA) have been shown to be the closest relatives of land plants. Three nuclear genomes (Spirogloea muscicola, Mesotaenium endlicherianum, and Penium margaritaceum) of ZGA have been recently published, and more genomes are underway. Here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating −) and found distinct cell sizes and other morphological differences. The molecular identities of the two strains were further investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a were surprisingly much more similar to Z. cylindricum (SAG 698-2) than to SAG 698-1b. …
Historical Contingency In Microbial Resilience To Hydrologic Perturbations, Hyun-Seob Song, James C. Stegen, Emily B. Graham, Timothy D. Scheibe
Historical Contingency In Microbial Resilience To Hydrologic Perturbations, Hyun-Seob Song, James C. Stegen, Emily B. Graham, Timothy D. Scheibe
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Development of reliable biogeochemical models requires a mechanistic consideration of microbial interactions with hydrology. Microbial response to and its recovery after hydrologic perturbations (i.e., resilience) is a critical component to understand in this regard, but generally difficult to predict because the impacts of future events can be dependent on the history of perturbations (i.e., historical contingency). Fundamental issues underlying this phenomenon include how microbial resilience to hydrologic perturbations is influenced by historical contingency and how their relationships vary depending on the characteristics of microbial functions. To answer these questions, we considered a simple microbial community composed of two species that …
Decline Of Novel Ecosystems Used By Endangered Species: The Case Of Piping Plovers, Least Terns, And Aggregate Mines, Joel G. Jorgensen, Stephen J. Brenner, Lauren R. Greenwalt, Mark P. Vrtiska
Decline Of Novel Ecosystems Used By Endangered Species: The Case Of Piping Plovers, Least Terns, And Aggregate Mines, Joel G. Jorgensen, Stephen J. Brenner, Lauren R. Greenwalt, Mark P. Vrtiska
School of Natural Resources: Faculty Publications
Sand and gravel mining creates novel ecosystems along the Platte, Loup, and Elkhorn rivers in Nebraska, USA. Piping plovers and least terns are state and/or federally threatened and endangered species, respectively, that nest and raise young at these sites and their derivatives. Despite hosting relatively large numbers of piping plovers and least terns for decades, an important question that has largely gone unaddressed is whether the industry that has produced these novel ecosystems is stable and will continue to produce habitat consistently in the future. We evaluated how the number, size, and spatial distribution of different site types hosting different …
Flash Drought Onset Over The Contiguous United States: Sensitivity Of Inventories And Trends To Quantitative Definitions, Mahmoud Osman, Bejamin F. Zaitchik, Hamada S. Badr, Jordan I. Christian, Tsegaye Tadesse, Jason A. Otkin, Martha C. Anderson
Flash Drought Onset Over The Contiguous United States: Sensitivity Of Inventories And Trends To Quantitative Definitions, Mahmoud Osman, Bejamin F. Zaitchik, Hamada S. Badr, Jordan I. Christian, Tsegaye Tadesse, Jason A. Otkin, Martha C. Anderson
National Drought Mitigation Center: Faculty Publications
The term “flash drought” is frequently invoked to describe droughts that develop rapidly over a relatively short timescale. Despite extensive and growing research on flash drought processes, predictability, and trends, there is still no standard quantitative definition that encompasses all flash drought characteristics and pathways. Instead, diverse definitions have been proposed, supporting wide-ranging studies of flash drought but creating the potential for confusion as to what the term means and how to characterize it. Use of different definitions might also lead to different conclusions regarding flash drought frequency, predictability, and trends under climate change. In this study, we compared five …
Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts, Amy N. Abrams, T. G. Mcdaneld, John W. Keele, Carol G. Chitko-Mckown, Larry A. Kuehn, Michael G. Gonda
Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts, Amy N. Abrams, T. G. Mcdaneld, John W. Keele, Carol G. Chitko-Mckown, Larry A. Kuehn, Michael G. Gonda
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole …
Modeling The Release And Spreading Of Permanganate From Aerated Slow-Release Oxidants In A Laboratory Flow Tank, Ann Kambhu, Yusong Li, Troy E. Gilmore, Steve D. Comfort
Modeling The Release And Spreading Of Permanganate From Aerated Slow-Release Oxidants In A Laboratory Flow Tank, Ann Kambhu, Yusong Li, Troy E. Gilmore, Steve D. Comfort
School of Natural Resources: Faculty Publications
Aerated, slow-release oxidants are a relatively new technology for treating contaminated aquifers. A critical need for advancing this technology is developing a reliable method for predicting the radius of influence (ROI) around each drive point. In this work, we report a series of laboratory flow tank experiments and numerical modeling efforts designed to predict the release and spreading of permanganate from aerated oxidant candles (oxidant-wax composites). To mimic the design of the oxidant delivery system used in the field, a double screen was used in a series of flow tank experiments where the oxidant was placed inside the inner screen …