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The Genome Of The Stable Fly, Stomoxys Calcitrans, Reveals Potential Mechanisms Underlying Reproduction, Host Interactions, And Novel Targets For Pest Control, Pia U. Olafson, Serap Aksoy, Geoffrey M. Attardo, Greta Buckmeier, Xiaoting Chen, Craig J. Coates, Megan Davis, Justin Dykema, Scott J. Emrich, Markus Friedrich, Christopher J. Holmes, Panagiotis Ioannidis, Evan N. Jansen, Emily C. Jennings, Daniel Lawson, Ellen O. Martinson, Gareth L. Maslen, Richard P. Meisel, Terence D. Murphy, Dana Nayduch, David R. Nelson, Kennan J. Oyen, Tyler J. Raszick, José M.C. Ribeiro, Hugh M. Robertson, Andrew J. Rosendale, Timothy B. Sackton, Perot Saelao, Sonja L. Swiger, Sing Hoi Sze, Aaron M. Tarone, David B. Taylor, Wesley C. Warren, Robert M. Waterhouse, Matthew T. Weirauch, John H. Werren, Richard K. Wilson, Evgeny M. Zdobnov, Joshua B. Benoit

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. Results: This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were …

Agent Based Modeling Of The Spread Of Social Unrest Based On Infectious Disease Spread Model, Anup Adhikari

School of Computing: Dissertations, Theses, and Student Research

Social unrest activities are the tools for people to show dissatisfaction, and often people are motivated by similar unrest activities in another region. This causes a spread of unrest activities across space and time. In this thesis, we model the spread of social unrest across time and space. The underlying novel methodology is to model the regions as agents that transition from one state to another based on changes in their environment. The methodology involves (1) creating a region vector for each agent based on socio-demographic, cultural, economic, infrastructural, geographic, and environmental (SCEIGE) factors, (2) formulating neighborhood distance function to …

Genome Annotation Using Average Mutual Information, Garin P. Newcomb

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

Advancements in high-throughput DNA sequencing technologies and ambitious goals for their use are resulting in the generation of a deluge of unannotated sequenced genomes. This makes computational tools that can aid in annotation increasingly valuable.

Here, we provide a detailed exploration of the utility as well as the limitations of average mutual information (AMI) in several steps of genome annotation. For a genomic sequence, AMI is a measure of the information a base contains about the base separated by a fixed lag. A profile is constructed by calculating AMI at multiple lags. In addition to traditional AMI, we employ two …

Detecting And Evaluating Cracks On Aging Concrete Members With Deep Convolutional Neural Networks, Brendan Barnes

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Cracks in concrete structures are evaluated through a timely and subjective manual inspection. The location of cracks is often recorded in an inspection report where some cracks are measured. Although measurements or locations may not be necessary for all cracks observed in concrete members, if quantitative data can be gathered in an autonomous way, allowing measurement data to be used in tracking changes in spatial and temporal scales, this quantitative data can provide useful information not yet captured in the manual inspection process. This thesis aims to construct an image-based crack detection and evaluation pipeline that can assist health monitoring …

Continuous Turbidity Data Used To Compute Constituent Concentrations In The South Loup River, Nebraska, 2017–18, David L. Rus, Brenda K. Densmore

United States Geological Survey: Water Reports and Publications

The South Loup River in central Nebraska has been impaired by bacteria since at least 2004, which has resulted in the river not meeting its intended use as a recreational waterway. As part of a strategy for reducing the bacterial load in the river, the United States Geological Survey (USGS), in cooperation with the Lower Loup Natural Resources District, made continuous estimates of Escherichia coli (E. coli) and nutrient concentrations during seasonal monitoring at the South Loup River at Saint Michael, Nebraska, during 2017–18. Continuous turbidity data were collected from mid-April through October in 2017 and 2018 …

American Burying Beetle, Plant Richness, And Soil Property Responses To Collapse Of Juniperus Virginiana Woodlands With Fire, Alison K. Ludwig

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grasslands are declining in the Great Plains due to land use changes, woody plant encroachment, and loss of historic fire cycles. Prescribed burn associations have utilized prescribed fire to collapse invading woodlands and allow the restoration of grasslands. This fire is considered “extreme” because it is capable of changing the structure and function of an ecosystem. Our study site is the Loess Canyons Experimental Landscape, a long-term, ecoregion-scale experiment to apply prescribed fire across the region to restore grasslands. The Nebraska Natural Legacy Project established the Loess Canyons ecoregion as a Biologically-Unique Landscape in 2005 with the state’s wildlife action …

Livestock Grazing Impacts On Crop And Soil Responses For Two Cropping Systems, Alyssa Kuhn

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Diversified crop, forage, and livestock systems are assumed to be more sustainable and economically competitive than traditional cropping systems. Objectives of this study were to determine effects of integrating grazing livestock into corn (Zea mays)-soybean (Glycine max (L.) Merr.) (C-S) and corn-soybean-wheat (Triticum aestivum L.) (C-S-W) cropping systems on plant population, grain yield, soil nutrients and soil carbon dioxide (CO₂) flux following winter grazing corn residue (both systems) and an oat (Avena sativa) cover crop (C-S-W only) planted after wheat. For the 2019 and 2020 production seasons, neither corn nor soybean plant …

The Complicated Road From Academic Dismissal To Degree Completion: A Phenomenological Exploration Of The Student Experience, Annmarie Gottner

Department of Educational Administration: Dissertations, Theses, and Student Research

Graduation rates are a key metric for measuring success, both for the student who attains their degree and the higher education institution that awards the diploma. In a time of increasing social pressure to demonstrate the value of the higher education enterprise, colleges and universities have become increasingly attentive to the graduation rates for their students. Individual students are sensitive to their likelihood of degree completion as costs associated with higher education increase alongside continued perception that employability is positively impacted by a college diploma. Faculty and administrators are keenly committed to supporting students to graduation success, and the economic …

The Importance Of Acquisition Learning On Nicotine And Varenicline Drug Substitution In A Drug-Discriminated Goal-Tracking Task, Brady M. Thompson, Scott T. Barrett, Y. Wendy Huynh, David A. Kwan, Jennifer E. Murray, Rick A. Bevins

Department of Psychology: Faculty Publications

Nicotine and varenicline (Chantix®; the leading non-nicotine cessation pharmacotherapy) can come to control appetitive behaviors such as goal-tracking. We tested rats (N = 48) in a drug-discriminated goal-tracking (DGT) task where each rat received daily subcutaneous injections of either nicotine (0.4 mg/kg) or saline (0.9% [w/v]) interspersed across the acquisition phase (Phase 1). On saline days, sucrose was intermittently available. On nicotine days, sucrose was withheld. All rats acquired the discrimination with increased goal-tracking rates on saline days relative to nicotine days. Following acquisition, rats were separated into four groups to assess drug-substitution and discrimination reversal in Phase 2. The …

An Intersectionality Framework On The Role Of Student-Teacher Relationships On Student Social Outcomes, Kenji Madison

College of Education and Human Sciences: Dissertations, Theses, and Student Research

This research aimed to build on the great wealth of knowledge on student-teacher relationships, social competence, and peer outcomes while utilizing a different approach integrating race and gender. The view of intersectionality may influence those who join in reading this research to consider race coupled with student gender as a strong influence affecting the formation of a relationship as instrumental as the student-teacher relationship.

In a sample of 10,886 (8489 White, 2397 Black) students from third to fifth grade, the research completed three aims: characterizing the relationship between teacher rated closeness and conflict and students’ social outcomes in fourth and …

The Impact Of Beneficial Organisms In Corn Agroecosystems, Callie Rosalind Braley

Doctor of Plant Health Program: Dissertations and Student Research

Corn is one of the world’s, and Nebraska’s, most important crops. Millions of acres are planted to corn each year in the Cornhusker State. However, each year there are a plethora of arthropod, weed, and microorganism pests that rob farmers of reaching their maximum yield potential. There are many options available to manage these pests in corn agroecosystems, but one option is often underutilized: beneficial organisms. For each pest, there are a variety of natural enemies that can assist in mitigating the damage caused by pests.

Many beneficial organisms exist, and they can be grouped by the type of pest …

Intricacies In Agronomic Management: The Role Of Interdisciplinary Education, Adam M. Striegel

Doctor of Plant Health Program: Dissertations and Student Research

As a science, agronomy is built upon the connection of inter-disciplinary fields of study. Management (M) of various discipline considerations (and their subsequent interactions) can be influenced by and have significant effects on genetic by environment (GxE) expression. This has led to the promotion of GxExM systems. However, optimizing GxExM programs requires extensive, interdisciplinary knowledge. To evaluate interdisciplinary training provided in undergraduate education, 11 four-year universities were selected in the United States that offer baccalaureate degree majors in agronomy or crop science. Surveys of undergraduate programs of study were conducted, with all required coursework separated into general degree components (general …

Examination Of Cajeput Oil (Melaleuca Cajuputi) Phytochemicals As Tools To Manage The Yellow Fever Mosquito (Aedes Aegypti L.), Ellis Johnson

Department of Entomology: Dissertations, Theses, and Student Research

The yellow fever mosquito, Aedes aegypti L., is among the most important insects as it pertains to public health. It vectors several health-endangering pathogens, including yellow fever, dengue fever, chikungunya, and Zika viruses. Insecticides are among the most effective tools used to manage mosquito populations however, their efficacy to manage mosquito populations is being threatened by insecticide resistance. This ongoing threat warrants new chemical strategies to reduce or incapacitate a mosquito population capable of pathogen transmission. Plant essential oils are candidates for identifying novel management tools for disease-vectoring arthropods. Commercially, essential oils have been used for pharmaceuticals, flavoring, fragrances, cosmetics, …

A Method For Visualizing Water Flow Through Modified Root Zones, Dallas M. Williams

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As the number of impervious surfaces in urban environments increases, the ability of modified root zones to infiltrate water is becoming more important. Current methods of tracing water flow through soil profiles include excavating large pits in situ or analyzing soil cores in the laboratory with computed tomography or magnetic resonance imaging. While useful, these methods may not be suitable for urban settings or practical in every laboratory. We propose a new method that is less invasive, does not require extensive technical equipment and can reliably trace water movement through the soil profile in order to calculate flow rate based …

Evidence Of Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Field-Evolved Resistance To Cry3bb1 + Cry34/35ab1 Maize In Nebraska, Jordan Reinders, Emily E. Reinders, Emily Robinson, Brian W. French, Lance J. Meinke

Department of Entomology: Faculty Publications

BACKGROUND: Western corn rootworm (WCR; Diabrotica virgifera virgifera) field-evolved resistance to transgenic maize expressing the Cry3Bb1 protein derived from Bacillus thuringiensis (Bt) has been confirmed across the United States Corn Belt. Although use of pyramided hybrids expressing Cry3Bb1 + Cry34/35Ab1 has increased in recent years to mitigate existing WCR Bt resistance, susceptibility of Nebraska WCR populations to this rootworm–Bt pyramid has not been assessed. Plant-based bioassays were used to characterize the susceptibility of WCR populations to Cry3Bb1 and Cry3Bb1 + Cry34/35Ab1 maize. Populations were collected from areas of northeastern Nebraska with a history of planting Bt maize that expressed Cry3Bb1 …

Economic Estimates Of Wild Hog (Sus Scrofa) Damage And Control Among Young Forest Plantations In Alabama, Micah Fern, Rebecca Barlow, Chris Slootmaker, John Kush, Stephanie Shwiff, Larry Teeter, Jim Armstrong

United States Department of Agriculture Wildlife Services: Staff Publications

Operating as ecological engineers, the increased distribution and abundance of wild hogs (Sus scrofa) has caused considerable socio-economic impacts. The international scope of economic research providing wild hog damage estimates are often confined to agricultural crops, while damage estimates among forest plantations are lacking. In Alabama, private landowners hold the majority of timberland acreage and are less equipped to absorb financial losses from wild hog damage than their industrial counterparts. A survey was conducted to estimate the economic impact of wild hogs, namely costs of damage and control, to privately owned forestlands. The survey was distributed in the …

Distillers' Grains: Past, Present, And Future Economic Analyses, Daniel E. Gertner

Department of Agricultural Economics: Dissertations, Theses, and Student Research

This thesis is comprised of four chapters, each of which discusses or conducts economic research related to the distillers’ grains market. The first three chapters are meant to be standalone papers. Chapter four provides potential paths forward in distillers’ grains research based on the findings of the first three chapters and concludes the thesis.

The first chapter conducts a comprehensive literature review that categorizes and summarizes economic research on distillers’ grains products. This section shows how the physical market has moved beyond the current academic understanding of market products and structure. Existing research finds that traditional distillers’ grains products positively …

Evaluation Of A Novel Approach For Assessing Biological Activity In Agricultural Soils, Karla Melgar

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Soil health is a key factor impacting soil resilience and fertility in crop production systems. Favorable soil physical and biological conditions facilitate plant nutrient absorption and nutrient cycling. Demonstrating to farmers the impacts and changes in soil biological activity under different soil management practices has been a challenge due to the limited availability of inexpensive tools for quantifying this component of soil health. The primary goal of this study was to present a simple and readily accessible tool for evaluating soil biological activity to promote the use of organic amendments in crop fields. Research plots were established in two studies …

Deterring Non-Target Birds From Toxic Bait Sites For Wild Pigs, Nathan P. Snow, Joseph M. Halseth, Justin A. Foster, Michael J. Lavelle, Justin W. Fischer, Michael P. Glow, Ingrid A. Messer, Seth M. Cook, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Toxic baiting of wild pigs (Sus scrofa) is a potential new tool for population control and damage reduction in the US. Field trials testing a prototype toxic bait (HOGGONE 2 containing 5% sodium nitrite [SN]), though, revealed that wild pigs spilled small particles of toxic bait outside of bait stations which subsequently created hazards for non-target species that consumed those particles, primarily passerine birds. To deter non-target birds from consuming particles of spilled bait, we tested four deterrents at mock bait sites (i.e., baited with bird seed) in north-central Colorado, USA during April–May 2020. We found a programable, inflatable deterrent …

Responses Of Turkey Vultures To Unmanned Aircraft Systems Vary By Platform, Morgan B. Pfeiffer, Bradley F. Blackwell, Thomas W. Seamans, Bruce N. Buckingham, Joshua L. Hoblet, Patrice E. Baumhardt

United States Department of Agriculture Wildlife Services: Staff Publications

A challenge that conservation practitioners face is manipulating behavior of nuisance species. The turkey vulture (Cathartes aura) can cause substantial damage to aircraft if struck. The goal of this study was to assess vulture responses to unmanned aircraft systems (UAS) for use as a possible dispersal tool. Our treatments included three platforms (fixed-wing, multirotor, and a predator-like ornithopter [powered by flapping flight]) and two approach types (30 m overhead or targeted towards a vulture) in an operational context. We evaluated perceived risk as probability of reaction, reaction time, flight-initiation distance (FID), vulture remaining index, and latency to return. …

Cardenolide, Potassium, And Pyrethroid Insecticide Combinations Reduce Growth And Survival Of Monarch Butterfly Caterpillars (Lepidoptera: Nymphalidae), Annie J. Krueger, Emily A. Robinson, Thomas J. Weissling, Ana M. Vélez, Troy D. Anderson

Department of Entomology: Faculty Publications

The monarch butterfly, Danaus plexippus L., has evolved to be insensitive to milkweed cardenolides via genetic modifications of Na⁺/K⁺-ATPase. There is concern for insecticide exposures near agriculture, with little information on monarch caterpillar toxicology. It is unclear how cardenolide insensitivity may affect the sensitivity of monarch caterpillars to pyrethroid insecticides. Additionally, potassium fertilizers may affect monarch caterpillar physiology and cardenolide sequestration. Here, we investigated the growth, survival, and development of caterpillars exposed to the cardenolide ouabain, bifenthrin, and potassium chloride (KCl) alone and in combination. Caterpillars were either exposed to (1) ouabain from third- to fifth-instar …

Landscape Transformations Produce Favorable Roosting Conditions For Turkey Vultures And Black Vultures, Jacob E. Hill, Kenneth F. Kellner, Bryan M. Kluever, Michael L. Avery, John S. Humphrey, Eric A. Tillman, Travis L. Devault, Jerrold L. Belant

United States Department of Agriculture Wildlife Services: Staff Publications

Recent increases in turkey vulture (Cathartes aura) and black vulture (Coragyps atratus) populations in North America have been attributed in part to their success adapting to human-modified landscapes. However, the capacity for such landscapes to generate favorable roosting conditions for these species has not been thoroughly investigated. We assessed the role of anthropogenic and natural landscape elements on roosting habitat selection of 11 black and 7 turkey vultures in coastal South Carolina, USA using a GPS satellite transmitter dataset derived from previous research. Our dataset spanned 2006–2012 and contained data from 7916 nights of roosting. Landscape …

Sex And Nest Type Influence Avian Blood Parasite Prevalence In A High-Elevation Bird Community, Marina D. Rodriguez, Paul F. Doherty, Antoinette J. Piaggio, Kathryn P. Huyvaert

United States Department of Agriculture Wildlife Services: Staff Publications

Background: The prevalence of avian haemosporidian parasites and the factors influencing infection in the Colorado Rocky Mountains are largely unknown. With climate change expected to promote the expansion of vector and avian blood parasite distributions, baseline knowledge and continued monitoring of the prevalence and diversity of these parasites is needed.

Methods: Using an occupancy modeling framework, we conducted a survey of haemosporidian parasite species infecting an avian community in the Colorado Rocky Mountains in order to estimate the prevalence and diversity of blood parasites and to investigate species-level and individual-level characteristics that may influence infection.

Results: We estimated the prevalence …

Potential Use For Serosurveillance Of Feral Swine To Map Risk For Anthrax Exposure, Texas, Usa, Rachel M. Maison, Courtney F. Pierce, Izabela K. Ragan, Vienna R. Brown, Michael J. Bodenchuk, Richard A. Bowen, Angela M. Bosco-Lauth

United States Department of Agriculture Wildlife Services: Staff Publications

Anthrax is a disease of concern in many mammals, including humans. Management primarily consists of prevention through vaccination and tracking clinical-level observations because environmental isolation is laborious and bacterial distribution across large geographic areas diffi cult to confi rm. Feral swine (Sus scrofa) are an invasive species with an extensive range in the southern United States that rarely succumbs to anthrax. We present evidence that feral swine might serve as biosentinels based on comparative seroprevalence in swine from historically defi ned anthrax-endemic and non–anthraxendemic regions of Texas. Overall seropositivity was 43.7% (n = 478), and logistic regression revealed county endemicity …

Publisher Correction: The Genome Of The Stable Fly, Stomoxys Calcitrans, Reveals Potential Mechanisms Underlying Reproduction, Host Interactions, And Novel Targets For Pest Control (Bmc Biology, (2021), 19, 1, (41), 10.1186/S12915-021-00975-9), Pia U. Olafson, Serap Aksoy, Geoffrey M. Attardo, Greta Buckmeier, Xiaoting Chen, Craig J. Coates, Megan Davis, Justin Dykema, Scott J. Emrich, Markus Friedrich, Christopher J. Holmes, Panagiotis Ioannidis, Evan N. Jansen, Emily C. Jennings, Daniel Lawson, Ellen O. Martinson, Gareth L. Maslen, Richard P. Meisel, Terence D. Murphy, Dana Nayduch, David R. Nelson, Kennan J. Oyen, Tyler J. Raszick, José M.C. Ribeiro, Hugh M. Robertson, Andrew J. Rosendale, Timothy B. Sackton, Perot Saelao, Sonja L. Swiger, Sing Hoi Sze, Aaron M. Tarone, David B. Taylor, Wesley C. Warren, Robert M. Waterhouse, Matthew T. Weirauch, John H. Werren, Richard K. Wilson, Evgeny M. Zdobnov, Joshua B. Benoit

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Following publication of the original article [1], it was reported that the article copyright was incorrect. The correct copyright statement is: © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021. The original article [1] has been corrected.

Genetic Fingerprinting And Aflatoxin Production Of Aspergillus Section Flavi Associated With Groundnut In Eastern Ethiopia, Abdi Mohammed, Paola C. Faustinelli, Alemayehu Chala, Mashilla Dejene, Chemeda Fininsa, Amare Ayalew, Chris O. Ojiewo, David A. Hoisington, Victor S. Sobolev, Jaime Martínez-Castillo, Renee S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Aspergillus species cause aflatoxin contamination in groundnut kernels, being a health threat in agricultural products and leading to commodity rejection by domestic and international markets. Presence of Aspergillus flavus and A. parasiticus colonizing groundnut in eastern Ethiopia, as well as presence of aflatoxins have been reported, though in this region, no genetic studies have been done of these species in relation to their aflatoxin production. Results: In this study, 145 Aspergillus isolates obtained from groundnut kernels in eastern Ethiopia were genetically fingerprinted using 23 Insertion/Deletion (InDel) markers within the aflatoxin-biosynthesis gene cluster (ABC), identifying 133 ABC genotypes. Eighty-four isolates …

Frontline Job Redesign Louisiana - Implementation Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The Implementation Team

The QIC-WD worked with the Louisiana Department of Children and Family Services (DCFS), Child Welfare Division (CWD) to establish an Implementation Team to lead the development and implementation of their intervention. The Implementation Team included the Site Implementation Manager (SIM); the project Training and Coaching Specialist; the Data Coordinator; representatives from Human Resources (HR), Civil Service and the Department’s training unit; representatives from the intervention parishes (from administrators to frontline workers); and three members of the QIC-WD (representing expertise in workforce, implementation, and evaluation). Members were selected for their leadership abilities, knowledge, experience, and their willingness …

Small Grain Cereal Cover Crops For Late Fall Or Early Spring Grazing, Kallie J. Calus

Department of Animal Science: Dissertations, Theses, and Student Research

Beef cattle operations are confronted with early spring and late fall forage deficiencies. Producers in integrated crop and livestock systems can fill forage gaps using cover crops as a forage source in between cash crops.

A five-year study evaluating forage production, growing calf performance and economics of grazing an oats cover crop planted after corn silage (CS) and high moisture corn (HMC) corn harvests was conducted. The economic analysis accounted for costs of establishing and grazing the oats and the value of calf gain to determine fall grazing system profitability. Steers had greater average daily gain grazing oats after CS …

Management Of Late Summer Planted Annual Forages For Grazing And The Impacts Of Novel Sweet Bran Plus Products On Performance And Carcass Characteristics Of Beef Finishing Steers, Devin Anthony Jakub

Department of Animal Science: Dissertations, Theses, and Student Research

Two separate grazing studies were conducted to evaluate: 1) the effects of rapeseed inclusion into late summer planted oats on the performance of growing steers during late fall and winter (Exp. 1) and 2) the effects of forage allocation on forage utilization and performance of steers grazing a late summer planted oat-rapeseed mix (Exp. 2). Variation in the corn wet milling process can result in negative effects on animal performance, nonetheless, there remains interest in further exploration and refinement of corn wet milling byproducts to achieve optimal byproduct compositions and cattle performance. Thus, another experiment was conducted to evaluate the …

Impact Of Wood-Sourced Biochar On Carbon And Nitrogen Capture In Beef Feedlot Systems, Jessica L. Sperber

Department of Animal Science: Dissertations, Theses, and Student Research

A feedlot growing and finishing experiment evaluated the effect of including pine-sourced biochar at 0.8 (growing) and 1.0% (finishing) of dietary DM on steer performance, carcass characteristics, and greenhouse gas (GHG) production (Exp 1). Two nutrient mass balance experiments were conducted during winter and summer seasons to evaluate the effect of spreading unprocessed red cedar biochar on the feedlot pen surface on manure nutrient capture and cattle performance (Exp 2). In Exp. 1, the inclusion of biochar in the growing diet did not impact steer performance. The inclusion of biochar in the finishing diet significantly reduced intake and gain, resulting …