Digital Commons Network^™

Getting Ahead Of Flash Drought: From Early Warning To Early Action, Jason A. Otkin, Molly Woloszyn, Hailan Wang, Mark D. Svoboda, Marina Skumanich, Roger Pulwarty, Joel Lisonbee, Andrew Hoell, Mike Hobbins, Tonya Haigh, Amanda E. Cravens

National Drought Mitigation Center: Faculty Publications

Flash droughts, characterized by their unusually rapid intensification, have garnered increasing attention within the weather, climate, agriculture, and ecological communities in recent years due to their large environmental and socioeconomic impacts. Because flash droughts intensify quickly, they require different early warning capabilities and management approaches than are typically used for slower-developing “conventional” droughts. In this essay, we describe an integrated research-and-applications agenda that emphasizes the need to reconceptualize our understanding of flash drought within existing drought early warning systems by focusing on opportunities to improve monitoring and prediction. We illustrate the need for engagement among physical scientists, social scientists, operational …

Executive Functioning In Language Learning Disabilities: Defining Executive Functioning, Executive Functioning Deficits, And Clinical Intervention Strategies To Support Children, Kaylie Trumble

Honors Program: Senior Projects (Public)

This thesis aims to select a working definition of executive functioning (EF) to identify possible EF deficits and clinical interventions strategies to support children. Included are EF deficits, ways of assessing EF components, and intervention strategies for executive dysfunction in LLD. Scenarios are provided to demonstrate both matching strategies to a child and strength/deficit in EF, along with a sample intervention script.

Exploring The Direct Relationship Between Sustainability Leader Ecospirituality And Human Capital Sustainability Leadership Capacity: A Moderated Mediation Study, Krystal L. Gabel

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

The primary purpose of this quantitative study was to determine if a sustainability leader’s ecospirituality significantly impacts one’s human capital sustainability leadership. The secondary purpose of this study was to determine if one’s psychological capital mediates this relationship and if one’s environmental attitudes moderate the relationships between ecospirituality, human capital sustainability leadership, and psychological capital.

Participants in this study included sustainability leaders in top positions at their organizations in the four highest-ranked countries (Denmark, Sweden, Norway, and the United Kingdom) on the 2022 Climate Change Performance Index. These individuals had positions of chief sustainability officer, head of sustainability, or sustainability …

The Emerging Digital Infrastructure For Research In The Humanities, Donald J. Waters

Copyright, Fair Use, Scholarly Communication, etc.

This article advances the thesis that three decades of investments by national and international funders, combined with those of scholars, technologists, librarians, archivists, and their institutions, have resulted in a digital infrastructure in the humanities that is now capable of supporting end-to-end research workflows. The article refers to key developments in the epigraphy and paleography of the premodern period. It draws primarily on work in classical studies but also highlights related work in the adjacent disciplines of Egyptology, ancient Near East studies, and medieval studies. The argument makes a case that much has been achieved but it does not declare …

Professionalization And The Forgotten System: Observed Practices And Perceptions At The Intersection Of Informal And Formal Faculty Development, Janie Brennan, Todd Fernandez, Joe Tranquillo

To Improve the Academy: A Journal of Educational Development

The professionalization of disciplines often leads to formalization of disciplinary training. As professionalization occurs, informal training roles are typically supplanted as the normative method of training but can continue to exist in parallel with formal methods. As in other fields, the ongoing professionalization of faculty development has created significant formalization of training. While the positive impact of formalization is clear in many areas of faculty development, little is known about how formalization affected the informal roles, activities, and perceptions that served to train faculty. This exploratory study reports on data gathered at a workshop designed to document and share stories …

The Global Publishing Industry In 2021, World Intellectual Property Organization

Copyright, Fair Use, Scholarly Communication, etc.

Mixed forces shaped global publishing activity in 2021. The spread of different COVID-19 variants throughout the world still caused hardship and widespread social distancing. At the same time, the rollout of vaccines helped to contain the pandemic’s impact and enabled a recovery of the global economy, which grew by around 6 percent in 2021. The preliminary release of data from WIPO’s global survey of publishing activity shows that recovery extended to the publishing industry. Still, industry performance varies by country, reflecting the nature of book markets and other local economic forces.

The highest number of books published and deposited in …

Leveraging The Structure Of Dnaja1 To Discover Novel Potential Pancreatic Cancer Therapies, Heidi E. Roth, Aline De Lima Leite, Nicolas Y. Palermo, Robert Powers

Department of Chemistry: Faculty Publications

Pancreatic cancer remains one of the deadliest forms of cancer with a 5-year survival rate of only 11%. Difficult diagnosis and limited treatment options are the major causes of the poor outcome for pancreatic cancer. The human protein DNAJA1 has been proposed as a potential therapeutic target for pancreatic cancer, but its cellular and biological functions remain unclear. Previous studies have suggested that DNAJA10s cellular activity may be dependent upon its protein binding partners. To further investigate this assertion, the first 107 amino acid structures of DNAJA1 were solved by NMR, which includes the classical J-domain and its associated linker …

Evaluating A Suite Of Strategies For Reading Graphic Novels: A Confirmatory Case Study, Maribeth Nottingham, Barbara J. Mcclanahan, Howard Atkinson

SANE journal: Sequential Art Narrative in Education

This article describes a qualitative study conducted by two researchers who are teacher educators and a literacy coach in a private school. The purpose was to determine the effectiveness of a set of strategies for reading and teaching about graphic novels by examining the responses of three elementary-grade teachers in a reading workshop environment to a semester-long professional development. Challenges both expected and unexpected were encountered but results overall were positive for teachers. In addition, they provided guidance to researchers for using the strategies introduced in the professional development in further studies.

The Textual Gutter: How Gene Luen Yang Redefines The Gutter In Boxers & Saints To Tell A Transnational Tale, David Lucas Jr

SANE journal: Sequential Art Narrative in Education

This paper attempts to provide a new understanding of the gutter and how it is used to significant effect in Gene Luen Yang's, Boxers & Saints. This research draws upon the work of Scott McCloud to establish a framework for the theoretical applications of the gutter. Most prior research focuses on the gutter within the page. This article demonstrates how Yang pushes the concept of the gutter further by creating a new type of gutter that moves beyond the pages and across texts. Then the research attempts to demonstrate how the idea of the textual gutter heightens the transnational elements …

Access To Online Formative Assessments In Lower-Division Undergraduate Biology Courses: Investigating Barriers To Student Engagement, Allison M. Upchurch, Dana L. Kirkwood-Watts, Kathleen R. Brazeal, Lorey A. Wheeler, Brian A. Couch, Gabrielle B. Johnson, Sarah K. Spier

School of Biological Sciences: Faculty Publications

Instructors use a variety of online formative assessment (FA) activities to support learning outside class. Previous studies have revealed barriers for students in online courses, but little is known about the barriers students experience when completing online FA assignments. Understanding these barriers to access is critical to fostering more inclusive learning for all students. Using a framework from previous work in online learning, we examined student perceptions of online FA access with respect to five barrier categories: technical resources, instructor organization, social interactions, personal engagement, and learning environment. We developed and administered a survey to more than 1,200 undergraduate biology …

Establishment Of Invasive Black Carp (Mylopharyngodon Piceus) In The Mississippi River Basin: Identifying Sources And Year Classes Contributing To Recruitment, Gregory W. Whitledge, Patrick T. Kroboth, Duane C. Chapman, Quinton E. Phelps, Wes Sleeper, Jennifer Bailey, Jill A. Jenkins

United States Geological Survey: Staff Publications

Black Carp (Mylopharyngodon piceus) was imported to the USA to control aquaculture pond snails. This species has escaped captivity and occurs in parts of the Mississippi River, several tributaries, and floodplain lakes, which is concerning due to potential competition with native fishes and predation on native mussels, many of which are imperiled. However, Black Carp captures have primarily been incidental by commercial fishers, and evidence of reproduction in the wild is limited. The objectives of this study were to assess relative abundance of aquaculture- origin and wild Black Carp using ploidy and otolith stable isotope analysis, identify spatial …

Loss Of Num1-Mediated Cortical Dynein Anchoring Negatively Impacts Respiratory Growth, Antoineen J. White, Clare S. Harper, Erica M. Rosario, Jonathan V. Dietz, Hannah G. Addis, Jennifer L. Fox, Oleh Khalimonchuk, Laura L. Lackner

Department of Biochemistry: Faculty Publications

Num1 is a multifunctional protein that both tethers mitochondria to the plasma membrane and anchors dynein to the cell cortex during nuclear inheritance. Previous work has examined the impact loss of Num1-based mitochondrial tethering has on dynein function in Saccharomyces cerevisiae; here, we elucidate its impact on mitochondrial function. We find that like mitochondria, Num1 is regulated by changes in metabolic state, with the protein levels and cortical distribution of Num1 differing between fermentative and respiratory growth conditions. In cells lacking Num1, we observe a reproducible respiratory growth defect, suggesting a role for Num1 in not only maintaining mitochondrial …

Genetic Analysis Of Seed Traits In Sorghum Bicolor That Affect The Human Gut Microbiome, Quinnan Yang, Mallory J. Suhr Van Haute, Nate Korth, Scott E. Sattler, John Toy, James C. Schnable, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two …

Ecological Interactions Disrupted By Habitat Alteration In The Neotropics, Aniko Toth, Andrew Allen, S. Kathleen Lyons, John Alroy

School of Biological Sciences: Faculty Publications

Ecological interactions help determine the distribution of species across landscapes and play crucial roles in ecosystem services such as pollination, seed dispersal, and pest control1. Human disturbances, particularly habitat alteration, have the potential to modify or erase ecological interactions^2,3 and so jeopardise the processes they control. While examples of interactions becoming rewired under human influence have been recorded, studies of this process for speciose assemblages at regional to continental scales are uncommon⁴ and obstructed by logistical difficulties2. The consequences for ecological communities and people are therefore poorly understood. Here we show that human habitat alteration is associated with …

Next-Generation Technologies Unlock New Possibilities To Track Rangeland Productivity And Quantify Multi-Scale Conservation Outcomes, Caleb P. Roberts, David E. Naugle, Brady W. Allred, Victoria M. Donovan, Dillon T. Fogarty, Matthew O. Jones, Jeremy D. Maestas, Andrew C. Olsen, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Historically, relying on plot-level inventories impeded our ability to quantify large-scale change in plant biomass, a key indicator of conservation practice outcomes in rangeland systems. Recent technological advances enable assessment at scales appropriate to inform management by providing spatially comprehensive estimates of productivity that are partitioned by plant functional group across all contiguous US rangelands. We partnered with the Sage Grouse and Lesser Prairie-Chicken Initiatives and the Nebraska Natural Legacy Project to demonstrate the ability of these new datasets to quantify multi-scale changes and heterogeneity in plant biomass following mechanical tree removal, prescribed fire, and prescribed grazing. In Oregon’s sagebrush …

Deja Vu: Semantics-Aware Recording And Replay Of High-Speed Eye Tracking And Interaction Data To Support Cognitive Studies Of Software Engineering Tasks—Methodology And Analyses, Vlas Zyrianov, Cole S. Peterson, Drew T. Guarnera, Joshua Behler, Praxis Weston, Bonita Sharif Ph.D., Jonathan I. Maletic

School of Computing: Faculty Publications

The paper introduces a fundamental technological problem with collecting high-speed eye tracking data while studying software engineering tasks in an integrated development environment. The use of eye trackers is quickly becoming an important means to study software developers and how they comprehend source code and locate bugs. High quality eye trackers can record upwards of 120 to 300 gaze points per second. However, it is not always possible to map each of these points to a line and column position in a source code file (in the presence of scrolling and file switching) in real time at data rates over …

Two Causal Relationships Between The Planes Of Fact-Finding And Substantive Law, Henrique Carneiro

Nebraska Law Bulletin (Selected Issues)

This essay depicts two causal processes that demonstrate how, in the American legal system, the fact-finding plane is more fundamental than the substantive law (or substantive rights) plane. In the first process, a priori policy judgments about what facts should be available, and a priori epistemological claims about what facts can be available, drive expansions, contractions, and intentional reaffirmations of legal rights’ substantive scope. In the second process, factual wrinkles in individual cases are seized upon by judges and/or litigators and exercise an outsized influence on the development of substantive law. Although these processes are present across different areas of …

Sers Spectroscopy With Machine Learning To Analyze Human Plasma Derived Sevs For Coronary Artery Disease Diagnosis And Prognosis, Xi Huang, Bo Liu, Shenghan Guo, Weihong Guo, Ke Liao, Guoku Hu, Wen Shi, Mitchell Kuss, Michael J. Duryee, Daniel R. Anderson, Yongfeng Lu, Bin Duan

Department of Electrical and Computer Engineering: Faculty Publications

Coronary artery disease (CAD) is one of the major cardiovascular diseases and represents the leading causes of global mortality. Developing new diagnostic and therapeutic approaches for CAD treatment are critically needed, especially for an early accurate CAD detection and further timely intervention. In this study, we successfully isolated human plasma small extracellular vesicles (sEVs) from four stages of CAD patients, that is, healthy control, stable plaque, non-ST-elevation myocardial infarction, and ST-elevation myocardial infarction. Surface-enhanced Raman scattering (SERS) measurement in conjunction with five machine learning approaches, including Quadratic Discriminant Analysis, Support Vector Machine (SVM), K-Nearest Neighbor, Artificial Neural network, were then …

Sers Spectroscopy With Machine Learning To Analyze Human Plasma Derived Sevs For Coronary Artery Disease Diagnosis And Prognosis, Xi Huang, Bo Liu, Shenghan Guo, Weihong Guo, Ke Liao, Guoku Hu, Wen Shi, Mitchell Kuss, Michael J. Duryee, Daniel R. Anderson, Yongfeng Lu, Bin Duan

Department of Electrical and Computer Engineering: Faculty Publications

Coronary artery disease (CAD) is one of the major cardiovascular diseases and represents the leading causes of global mortality. Developing new diagnostic and therapeutic approaches for CAD treatment are critically needed, especially for an early accurate CAD detection and further timely intervention. In this study, we successfully isolated human plasma small extracellular vesicles (sEVs) from four stages of CAD patients, that is, healthy control, stable plaque, non-ST-elevation myocardial infarction, and ST-elevation myocardial infarction. Surface-enhanced Raman scattering (SERS) measurement in conjunction with five machine learning approaches, including Quadratic Discriminant Analysis, Support Vector Machine (SVM), K-Nearest Neighbor, Artificial Neural network, were then …

Cytogenetic And Genomic Characterization Of A Novel Tall Wheatgrass‑Derived Fhb7 Allele Integrated Into Wheat B Genome, Wei Zhang, Tatiana Danilova, Mingyi Zhang, Shuangfeng Ren, Xianwen Zhu, Qijun Zhang, Shaobin Zhong, Linda Dykes, Jason Fiedler, Steven Xu, Katherine Frels, Stephen Wegulo, Jeffrey Boehm Jr., Xiwen Cai

Department of Agronomy and Horticulture: Faculty Publications

A novel tall wheatgrass-derived (Thinopyrum elongatum, genome EE) Fhb7 allele, designated Fhb7^The2, was identified and integrated into the wheat B genome through a small 7B–7E translocation (7BS·7BL–7EL) involving the terminal regions of the long arms. Fhb7^The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7^The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum …

Protocol For Engineering And Validating A Synthetic Mitochondrial Intermembrane Bridge In Mammalian Cells, Gunjan Purohit, Martonio Ponte Viana, Oleh Khalimonchuk

Department of Chemical and Biomolecular Engineering: Faculty Publications

Membrane contact sites are recognized as critical means of intercompartmental communication. Here, we describe a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support functioning of the endogenous mitochondrial contact site and cristae organizing system (MICOS). A chimeric protein,MitoT, is stably expressed in cultured mammalian cells to bridge themitochondrial membranes. This approach can be a valuable tool to study the function of the MICOS complex and associated proteins.

How Digital Is Agriculture In A Subset Of Countries From South America? Adoption And Limitations, Laila A. Puntel, É. L. Bolfe, R. J. M. Melchiori, R. Ortega, G. Tiscornia, A. Roel, F. Scaramuzza, S. Best, A. G. Berger, D. S. S. Hansel, D. Palacios Durán, G. R. Balboa

Department of Agronomy and Horticulture: Faculty Publications

Digital agriculture (DA) can contribute solutions to meet an increase in healthy, nutritious, and affordable food demands in an efficient and sustainable way. South America (SA) is one of the main grain and protein producers in the world but the status of DA in the region is unknown. A systematic review and case studies from Brazil, Argentina, Uruguay, and Chile were conducted to address the following objectives: (1) quantify adoption of existing DA technologies, (2) identify limitations for DA adoption; and (3) summarise existing metrics to benchmark DA benefits. Level of DA adoption was led by Brazil and Argentina followed …

Multi-Transcriptome Analysis Following An Acute Skeletal Muscle Growth Stimulus Yields Tools For Discerning Global And Myc Regulatory Networks, Kevin A. Murach, Zhengye Liu, Baptiste Jude, Vandre C. Figueiredo, Yuan Wen, Sabin Khadgi, Seongkyun Lim, Francielly Morena Da Silva, Nicholas P. Greene, Johanna T. Lanner, John J. Mccarthy, Ivan Jose Vechetti Jr, Ferdinand Von Walden

Department of Nutrition and Health Sciences: Faculty Publications

Myc is a powerful transcription factor implicated in epigenetic reprogramming, cellular plasticity, and rapid growth as well as tumorigenesis. Cancer in skeletal muscle is extremely rare despite marked and sustained Myc induction during loading-induced hypertrophy. Here, we investigated global, actively transcribed, stable, and myonucleus-specific transcriptomes following an acute hypertrophic stimulus in mouse plantaris. With these datasets, we define global and Myc-specific dynamics at the onset of mechanical overload-induced muscle fiber growth. Data collation across analyses reveals an under-appreciated role for the muscle fiber in extracellular matrix remodeling during adaptation, along with the contribution of mRNA stability to epigenetic-related transcript …

Different Gut Microbiomes Of Developmental Stages Of Field-Collected Native And Invasive Western Bean Cutworm, Striacosta Albicosta, In Western Nebraska, Paul A. Ayayee, Austin Currie, Julie A. Peterson

Department of Entomology: Faculty Publications

While insects harbor gut microbial associates that perform various functions for the host, lepidopterans have not been considered as prime examples of having such relationships. The western bean cutworm, Striacosta albicosta (Lepidoptera: Noctuidae), is native to North America and has historically been a significant corn pest in its western distribution. It is currently expanding eastwards and is invasive in these new regions. Using 16S rRNA gene sequencing data, this study focused on characterizing the microbiota associated with field-collected eggs, larvae, adults, and host plant materials of S. albicosta in its native range. The diversity of microbiomes varied significantly among S. …

Spatially Guided Construction Of Multilayered Epidermal Models Recapturing Structural Hierarchy And Cell–Cell Junctions, Haiwei Zhai, Xiaowei Jin, Grayson Minnick, Jordan Rosenbohm, Mohammed Abdul Haleem Hafiz, Ruiguo Yang, Fanben Meng

Department of Mechanical and Materials Engineering: Faculty Publications

A current challenge in 3D bioprinting of skin equivalents is to recreate the distinct basal and suprabasal layers and promote their direct interactions. Such a structural arrangement is essential to establish 3D stratified epidermis disease models, such as for the autoimmune skin disease pemphigus vulgaris (PV), which targets the cell– cell junctions at the interface of the basal and suprabasal layers. Inspired by epithelial regeneration in wound healing, a method that combines 3D bioprinting and spatially guided self-reorganization of keratinocytes is developed to recapture the fine structural hierarchy that lies in the deep layers of the epidermis.Herein, keratinocyteladen fibrin hydrogels …

Ag-Iot For Crop And Environment Monitoring: Past, Present, And Future, Nipuna Chamara, Md Didarul Islam, Geng Bai, Yeyin Shi, Yufeng Ge

Department of Agricultural and Biological Systems Engineering: Faculty Publications

CONTEXT: Automated monitoring of the soil-plant-atmospheric continuum at a high spatiotemporal resolution is a key to transform the labor-intensive, experience-based decision making to an automatic, data-driven approach in agricultural production. Growers could make better management decisions by leveraging the real-time field data while researchers could utilize these data to answer key scientific questions. Traditionally, data collection in agricultural fields, which largely relies on human labor, can only generate limited numbers of data points with low resolution and accuracy. During the last two decades, crop monitoring has drastically evolved with the advancement of modern sensing technologies. Most importantly, the introduction …

Effects Of Nutrient Level And Growth Rate On The Conjugation Process That Transfers Mobile Antibiotic Resistance Genes In Continuous Cultures, Mohammadreza Shafieifini, Yuepeng Sun, Zachery R. Staley, University Of Nebraska-Lincoln, Jean-Jack Riethoven, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Bacteria in the effluent of wastewater treatment plants (WWTPs) can transfer antibiotic resistance genes (ARGs) to the bacteria in receiving water through conjugation; however, there is a lack of quantitative assessment of this phenomenon in continuous cultures. Our objective was to determine the effects of background nutrient levels in river water column and growth rates of bacteria on the conjugation frequency of ARGs from effluent bacteria to river bacteria, as well as on the resulting resistance level (i.e., MICs) of the river bacteria. Chemostats were employed to simulate the discharge points of WWTPs into rivers, where effluent bacteria (donor cells) …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Lessons Learned From The 2019 Nebraska Floods: Implications For Emergency Management, Mass Care, And Food Security, Eric E. Calloway, Nadine B. Nugent, Katie L. Stern, Ashley Mueller, Amy L. Yaroch

Nebraska Cooperative Extension: Faculty and Staff Publications

This qualitative study aimed to understand the actions, challenges, and lessons learned for addressing the food and water needs of flood survivors, with a special focus on vulnerable populations and the implications for food security, to inform future disaster response efforts in the U.S. Semi-structured in-depth interviews were conducted from January to August 2020 with the local, state, and national stakeholders (n = 27) involved in the disaster response to the 2019 Nebraska floods, particularly those involved in providing mass care, such as food, water, and shelter, for the flood survivors. The challenge themes were related to limited risk …