Digital Commons Network^™

Saturated Free Fatty Acids Induce Placental Trophoblast Lipoapoptosis, Sathish Kumar Natarajan, Taylor Bruett, Philma Glora Muthuraj, Prakash K. Sahoo, Jillian Power, Justin L. Mott, Corrine Hanson, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Obesity during pregnancy increases the risk for maternal complications like gestational diabetes, preeclampsia, and maternal inflammation. Maternal obesity also increases the risk of childhood obesity, intrauterine growth restriction (IUGR) and diabetes to the offspring. Increased circulating free fatty acids (FFAs) in obesity due to adipose tissue lipolysis induces lipoapoptosis to hepatocytes, cholangiocytes, and pancreatic-β-cells. During the third trimester of human pregnancy, there is an increase in maternal lipolysis and release of FFAs into the circulation. It is currently unknown if increased FFAs during gestation as a result of maternal obesity cause placental cell lipoapoptosis. Increased exposure of FFAs during maternal …

Preclinical Development Of Single Walled Carbon Nanotube-Based Optical Biosensors, Eric M. Hofferber

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

High resolution, long-term monitoring of key biological analytes would improve patient outcomes by providing earlier detection of disease states and improved efficacy of treatment. One class of biosensors that have gained much attention in recent years are optical biosensors. Optical probes are attractive biosensors due to their noninvasive nature of detection, as certain light can pass through tissue, water, and blood. Single walled carbon nanotubes (SWNT) are a specific type of optical biosensor that fluoresce in the near infrared range of the electromagnetic spectrum and offer unparalleled spatial and temporal resolution. SWNT have been applied as biosensors in vitro, …

Seductive Details In Training, Megan Paul

Umbrella Summaries

What are seductive details?

In a learning environment, seductive details are interesting but unimportant details that are not necessary to achieve the instructional objective (Garner et al., 1989). The information may be tangentially related to the topic but is not relevant to the main teaching goal. Such details are often included for the purpose of making the topic more interesting and engaging. There are many potential types of seductive details; they can be visual, verbal, or aural—static or dynamic images (e.g., illustrations, photos, animations), written or spoken words (e.g., text, narration), or sounds (e.g., music; Sundararajan & Adesope, 2020).

Why …

Effects Of Farrowing Stall Layout And Number Of Heat Lamps On Sow And Piglet Behavior, Suzanne M. Leonard, Hongwei Xin, Tami M. Brown-Brandl, Brett C. Ramirez, Anna K. Johnson, Somak Dutta, Gary A. Rohrer

Department of Animal Science: Faculty Publications

Farrowing stalls are used in the United States swine industry to reduce pre-weaning piglet mortality, enable efficient individual animal management, and decrease facility construction and operating costs. The quantity and quality of space provided for sows and piglets in farrowing stalls are important economic and welfare considerations. To further explore the impacts of farrowing stall space allocation, a large-scale field study was conducted to compare sow and piglet behavior when housed in three farrowing stall layouts (TSL – traditional stall layout, ECSL – expanded creep area stall layout, ESCSL – expanded sow and creep area stall layout) with either one …

A Guide To Helminth Parasites Reported From Shorebirds (Charadriidae) From The Americas, Albert G. Canaris, Sofia Capasso, Gay J. Canaris

MANTER: Journal of Parasite Biodiversity

The shorebird family Charadriidae in the Americas consists of 21 native and 7 vagrant species. Members of the family occupy a diversity of open habitats, ranging from Arctic tundra during nesting, coastal sands, and mudflats to inland prairies, savannas, and wetlands. Some native plovers migrate from nesting grounds in North America to wintering grounds in South America (Hayman et al., 1986; Paulson, 2005; Winkler et al., 2020). Our search of the literature revealed the following: 17 of 28 host species infected with helminth parasites, 153 helminth species, and 199 infections involving 13 geographic areas. The purpose of this guide is …

Non-Nutritive Suck Burst Pattern Stability In Extremely Premature Infants, Alejandra Marquez

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The development of non-nutritive suck (NNS) burst dynamics in preterm infants reflects the integrity of the brain and is used clinically to assess feeding readiness and orofacial motor development (Mizuno and Ueda, 2005). The application of NNS analytics in the present report represents one outcome measurement set that is part of an ongoing clinical trial involving extremely preterm infants (EPI’s,[GA]) randomized to receive either pulsed orocutaneous stimulation therapeutics or a sham (blind pacifier), in conjunction with salivary sampling twice weekly to map gene expression of key proteins involved in neural development and molecular sensing of feeding related pathways in the …

Recovery Method For Mites Discovered In Mummified Human Tissue, Jessica Smith

Department of Anthropology: Theses and Student Research

Much like other arthropods, mites have been discovered in a wide variety of forensic and archaeological contexts featuring mummified remains. Their accurate identification has assisted forensic scientists and archaeologists in determining environmental, depositional, and taphonomic conditions that surrounded the mummified remains after death. Consequently, their close association with cadavers has led some researchers to intermittently advocate for the inclusion of mites in archaeological site analyses and forensic case studies. However, despite their potential value, mites have been underutilized with a variety of reasons for the lack of inclusion of mites in archaeological and forensic analyses. Chief amongst these reasons is …

A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee Mcfee Fee, Sarah Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp

Department of Animal Science: Faculty Publications

A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …

Clinical Case Study: The Effects Of Real-Time Iemg Biofeedback On Facial Muscle Activation Patterns In A Child With Congenital Facial Palsy, Alyssa Molina

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Experimental findings are limited concerning efficacious procedures for facial reanimation following persistent idiopathic facial paralysis or paresis. Additional research examining the use of real-time integrated electromyography (iEMG) biofeedback for young children with persistent idiopathic facial paralysis is one approach to further understand the efficacy of electrophysiology biofeedback techniques in facial reanimation for this age group and etiology. iEMG research has applications in interventions for pathology of nerves and muscles affecting animation of the face. The proposed clinical case study will adopt real time iEMG visual biofeedback to facilitate facial reanimation in a preadolescent child. Quantitative measurements will determine orofacial muscle …

Out Of This World Club!, Alec Miller, Katherine Osmundson

Honors Program: Expanded Learning Clubs

The purpose of Out of this World Club! was to make STEM and space less scary and intimidating, and display that anyone, even elementary students, can have fun learning about space. Each week consisted of learning about different planets in our solar system, their characteristics, and how they are similar or different from one another. The activities centered around creating something tangible that is representative of space. For example, the students designed asteroids with a rock and paint, created constellations out of toothpicks and mini marshmallows, and designed small rockets powered by blowing through a straw. A principal goal of …

Changes In Subcellular Localization Of Host Proteins Induced By Plant Viruses, Rosalba Rodriguez-Pena, Kaoutar El Mounadi, Hernan Garcia-Ruiz

Department of Plant Pathology: Faculty Publications

Viruses are dependent on host factors at all parts of the infection cycle, such as translation, genome replication, encapsidation, and cell-to-cell and systemic movement. RNA viruses replicate their genome in compartments associated with the endoplasmic reticulum, chloroplasts, and mitochondria or peroxisome membranes. In contrast, DNA viruses replicate in the nucleus. Viral infection causes changes in plant gene expression and in the subcellular localization of some host proteins. These changes may support or inhibit virus accumulation and spread. Here, we review host proteins that change their subcellular localization in the presence of a plant virus. The most frequent change is the …

Lydie Salvayre, Maintenant Même, Warren Motte, Lydie Salvayre, Bernard Wallet, David Lopez, Marie Cosnay, Mahir Guven, Stéphane Bikialo

Zea E-Books Collection

Warren Motte, «Dans le vif du vivant»

Lydie Salvayre et Warren Motte, «Une conversation avec Lydie Salvayre»

Lydie Salvayre, «Deux artistes»

Lydie Salvayre, «Projet en cours»

Lydie Salvayre, «Quatre photos»

Bernard Wallet, «Lydie Salvayre, écrivain baroque’n’roll»

David Lopez, «Almuerz»

Marie Cosnay, «Diamant brut»

Mahir Guven, «À propos de Lydie Salvayre»

Stéphane Bikialo, «Éloge de la fuite»

«Ouvrages de Lydie Salvayre»

Understanding Spaces Of Abandonment Through Virtual Frameworks In Landscape Architecture, Aus Perez

Honors Program: Senior Projects (Public)

In recent years, design professionals have implemented many contemporary landscape architecture projects across the United States. With a primary goal of returning nature to urban environments, contemporary landscape architects and other transdisciplinary partners work diligently to sculpt physical spaces that reflect the human-living experience. However, a leap into the world of video game design could allow landscape architects and urban planners to more freely create virtual social environments to address rising issues of abandonment in today’s urban and rural spaces. Video game mechanics and methodologies can be used extensively in the disciplines of design that value participatory processes, like landscape …

Wimmin In The Mass Media, Terry Nygren, Mary Jo Deegan

Zea E-Books Collection

Introduction to the 40th Anniversary Edition: Wimmin in the Mass Media and Centennial College, Looking Backwards • Mary Jo Deegan

WIMMIN IN THE MASS MEDIA: Articles Collected at the Centennial Education Program, Fall 1980

Introduction: Wimmin and the Mass Media — Construction of the Self • Mary Jo Deegan and Terry Nygren

Examining the Top Ten, or Why Those Songs Make the Charts • Jane Pemberton

Images of Women in Rock Music: Analysis of B-52’s and Black Rose• Sheila M. Krueger

Women in Sitcoms: “I Love Lucy”• Nancy Grant-Colson

Horatio Alger is Alive and Well and Masquerading as a Feminist, …

Considering The Use Of The Terms Strain And Adaptation In Prion Research, Robert M. Zink

School of Natural Resources: Faculty Publications

Evolutionary biologists and disease biologists use the terms strain and adaptation in Chronic Wasting Disease (CWD) research in different ways. In evolutionary biology, a strain is a nascent genetic lineage that can be described by a genealogy, and a phylogenetic nomenclature constructed to reflect that genealogy. Prion strains are described as showing distinct host range, clinical presentation, disease progression, and neuropathological and PrP biochemical profiles, and lack information that would permit phylogenetic reconstruction of their history. Prion strains are alternative protein conformations, sometimes derived from the same genotype. I suggest referring to prion strains as ecotypes, because the variant phenotypic …

Testing A Global Standard For Quantifying Species Recovery And Assessing Conservation Impact, Molly K. Grace, H Resit Akcakaya, Elizabeth L. Bennett, Thomas M. Brooks, Anna Heath, Simon Hedges, Craig Hilton-Taylor, Michael Hoffmann, Axel Hochkirch, Richard Jenkins, David A. Keith, Barney Long, David P. Mallon, Erik Meijaard, E.J. Milner-Gulland, Jon Paul Rodriguez, P J. Stephenson, Simon N. Stuart, Richard P. Young, Pablo Acebes, Joanna Alfaro-Shigueto, Silvia Alvarez-Clare, Raphali Rodlis Andriantsimanarilafy, Marina Arbetman, Claudio Azat, Gianluigi Bacchetta, Ruchi Badola, Luis M.D. Barcelos, Joao Pedro Barreiros, Sayanti Basak, Danielle J. Berger, Sabuj Bhattacharyya, Gilad Bino, Paulo A.V. Borges, Raoul K. Boughton, H Jane Brockmann, Hannah L. Buckley, Ian J. Burfield, James Burton, Teresa Camacho-Badani, Luis Santiago Cano-Alonso, Ruth H. Carmichael, Christina Carrero, John P. Carroll, Giorgos Catsadorakis, David G. Chapple, Guillaume Chapron, Gawsia Wahidunnessa Chowdhury, Louw Claassens, Donatella Cogoni, Rochelle Constantine, Christie Anne Craig, Andrew A. Cunningham, Nishma Dahal, Jennifer C. Daltry, Goura Chandra Das, Niladri Dasgupta, Alexandra Davey, Katharine Davies, Pedro Develey, Vanitha Elangovan, David Fairclough, Mirko Di Febbraro, Giuseppe Fenu, Fernando Moreira Fernandes, Eduardo Pinheiro Fernandez, Brittany Finucci, Rita Foldesi, Catherine M. Foley, Matthew Ford, Michael R.J. Forstner, Nestor Garcia, Ricardo Garcia-Sandoval, Penny C. Gardner, Roberto Garibay-Orijel, Marites Gatan-Balbas, Irene Gauto, Mirza Ghazanfar Ullah Ghazi, Stephanie S. Godfrey, Matthew Gollock, Benito A. Gonzalez, Tandora D. Grant, Thomas Gray, Andrew J. Gregory, Roy H.A. Van Grunsven, Marieka Gryzenhout, Noelle C. Guernsey, Garima Gupta, Christina Hagen, Christian A. Hagen, Madison B. Hall, Eric Hallerman, Kelly Hare, Tom Hart, Ruston Hartdegen, Yvette Harvey-Brown, Richard Hatfield, Tahneal Hawke, Claudia Hermes, Rod Hitchmough, Pablo Melo Hoffmann, Charlie Howarth, Michael A. Hudson, Syed Ainul Hussain, Charlie Huveneers, Helene Jacques, Dennis Jorgensen, Suyash Katdare, Lydia K.D. Katsis, Rahul Kaul, Boaz Kaundra-Arara, Lucy Keith-Diagne, Daniel T. Kraus, Thales Moreira De Lima, Ken Lindeman, Jean Linsky, Edward Louis Jr., Anna Loy, Eimear Nic Lughadha, Jeffrey C. Mangel, Paul E. Marinari, Gabriel M. Martin, Gustavo Martinelli, Philip J.K. Mcgowan, Alistair Mcinnes, Eduardo Teles Barbosa Mendes, Michael J. Millard, Claire Mirande, Daniel Money, Joanne M. Monks, Carolina Laura Morales, Nazia Noareen Mumu, Raquel Negrao, Anh Ha Nguyen, Nazmul Hasan Niloy Md., Grant Leslie Norbury, Cale Nordmeyer, Darren Norris, Mark O'Brien, Gabriela Akemi Oda, Simone Orsengio, Mark Evan Outerbridge, Stesha Pasachnik, Juan Carlos Perez-Jimenez, Charlotte Pike, Fred Pilkington, Glenn Plumb, Rita De Cassia Quitete Portela, Ana Prohaska, Manuel G. Quintana, Eddie Fanantenana Rakotondrasoa, Dustin H. Ranglack, Hassan Rankou, Ajay Prakash Rawat, James Thomas Reardon, Marcelo Lopes Rheingantz, Stephen C. Richter, Malin C. Rivers, Luke Rollie Rogers, Patricia Da Rosa, Paul Rose, Emily Royer, Catherine Ryan, Yvonne J. Sadovy De Mitcheson, Lily Salmon, Carlos Henrique Salvador, Michael J. Samways, Tatiana Sanjuan, Amanda Souza Dos Santos, Hiroshi Sasaki, Emmanuel Shutz, Heather Ann Scott, Robert Michael Scott, Fabrizio Serena, Surya P. Sharma, John A. Shuey, Carlos Julio Polo Silva, John P. Simaika, David R. Smith, Julia L.Y. Spaet, Shanjida Sultana, Bibhab Kumar Talukdar, Vikash Tatayah, Philip Thomas, Angela Tringali, Hoang Trinh-Dinh, Chongpi Tuboi, Aftab Alam Usami, Aida M. Vasco-Palacios, Jean-Christophe Vie, Jo Virens, Alan Walker, Bryan Wallace, Lauren J. Waller, Hongfeng Wang, Oliver R. Wearn, Merlijin Van Weerd, Simon Weigmann, Daniel Willcox, John Woinarski, Jean W.H. Yong, Stuart Young

School of Natural Resources: Faculty Publications

Recognizing the imperative to evaluate species recovery and conservation impact, in 2012 the International Union for Conservation of Nature (IUCN) called for development of a “Green List of Species” (now the IUCN Green Status of Species). A draft Green Status framework for assessing species’ progress toward recovery, published in 2018, proposed 2 separate but interlinked components: a standardized method (i.e., measurement against benchmarks of species’ viability, functionality, and preimpact distribution) to determine current species recovery status (herein species recovery score) and application of that method to estimate past and potential future impacts of conservation based on 4 metrics (conservation legacy, …

Understanding Perceptions Of Quality Among Early Childhood Education Stakeholders In Tanzania And Lesotho: A Multiple Qualitative Case Study, Dawn Davis, Debra Miller, Dorothy Mrema, Moikabi Matsoai, Ntsoaki Mapetla, Abbie Raikes, Anna Burton

Department of Child, Youth, and Family Studies: Faculty Publications

Recent global efforts around early childhood education (ECE) have led to increased investments and access, especially in low- and middle-income countries (UNESCO, 2019). As access grows, focus has shifted from enrollment to quality (Gove, 2017). This paper explores how ECE stakeholders in Tanzania and Lesotho define ECE quality. Findings show that stakeholders define quality in similar ways, highlighting the importance of trained teachers who implement specific teaching practices, strong partnerships with families and the community, critical infrastructure, and government support. However, review of the country contexts found that current conditions and support for these quality indicators were lacking.

Do Long-Term Conservation Pasture Management Practices Influence Microbial Diversity And Antimicrobial Resistant Genes In Runoff?, Yichao Yang, Amanda J. Ashworth, Lisa M. Durso, Mary Savin, Jennifer M. Debruyn, Kimberly Cook, Philip A. Moore Jr, Philip R. Owens

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Runoff from land-applied manure and poultry litter is one mechanism by which manure-borne bacteria are transported over large distances in the environment. There is a global concern that antimicrobial resistant (AMR) genes may be transmitted through the food chain from animal manures to soil to surface water. However, details are lacking on the ecology of AMR genes in water runoff as well as how conservation management practices may affect the runoff microbiome or minimize the movement of AMR genes. The aim of this study was to identify microbial community structure and diversity in water runoff following 14-years of poultry litter …

Isoprene Production From Municipal Wastewater Biosolids By Engineered Archaeon Methanosarcina Acetivorans, Sean Carr, Jared Aldridge, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Wastewater biosolids are a promising feedstock for production of value-added renewable chemicals. Methane-producing archaea (methanogens) are already used to produce renewable biogas via the anaerobic treatment of wastewater. The ability of methanogens to efficiently convert dissolved organic carbon into methane makes them an appealing potential platform for biorefining using metabolic engineering. We have engineered a strain of the methanogen Methanosarcina acetivorans to produce the volatile hemiterpene isoprene in addition to methane. The engineered strain was adapted to grow in municipal wastewater through cultivation in a synthetic wastewater medium. When introduced to municipal wastewater the engineered methanogens were able to compete …

Isoprene Production From Municipal Wastewater Biosolids By Engineered Archaeon Methanosarcina Acetivorans, Sean Carr, Jared Aldridge, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Wastewater biosolids are a promising feedstock for production of value-added renewable chemicals. Methane-producing archaea (methanogens) are already used to produce renewable biogas via the anaerobic treatment of wastewater. The ability of methanogens to efficiently convert dissolved organic carbon into methane makes them an appealing potential platform for biorefining using metabolic engineering. We have engineered a strain of the methanogen Methanosarcina acetivorans to produce the volatile hemiterpene isoprene in addition to methane. The engineered strain was adapted to grow in municipal wastewater through cultivation in a synthetic wastewater medium. When introduced to municipal wastewater the engineered methanogens were able to compete …

Pedagogical Agents, Megan Paul

Umbrella Summaries

What are pedagogical agents?

Pedagogical agents are “lifelike characters presented on a computer screen that guide users through multimedia learning environments” (Clarebout & Heidig, 2012, p. 2568). Pedagogical agents can vary in multiple ways. The following characteristics have been most studied: appearance (two-dimensional [2D] vs. three-dimensional [3D]), eye gaze, facial expression, gesturing, motion, and gender. The simplest pedagogical agent would be a static, 2D image with just text, and a complex agent would be a dynamic, 3D person or character that talks, gestures, and has eye movements and facial expressions.

Why are pedagogical agents valuable?

Pedagogical agents are valuable because …

Moderate Grazer Density Stabilizes Forage Availability More Than Patch Burning In Low-Stature Grassland, Edward J. Raynor, Devan Allen Mcgranahan, James R. Miller, Diane M. Debinski, Walter H. Schacht, David M. Engle

School of Natural Resources: Faculty Publications

Spatially patchy fire creates landscape-level diversity that in turn stabilizes several rangeland ecosystem services, including forage production and habitat availability. To enhance biodiversity and livestock production, efforts are underway to restore fire regimes in rangelands throughout the Great Plains. However, invasive species such as tall fescue Schedonorus arundinaceus syn. Festuca arundinacea, initially introduced for forage production, hamper prescribed fire use. Grazer density, or stocking rate, modulates the effect of patchy fire regimes on ecological patterns in invaded, seminatural rangeland pastures. We compare three diversity–stability responses—temporal variability in aboveground plant biomass, portfolio effects among plant functional groups, and beta diversity in …

Using Enclosed Y-Mazes To Assess Chemosensory Behavior In Reptiles, M. Rockwell Parker, Andrea F. Currylow, Eric A. Tillman, Charlotte J. Robinson, Jilian M. Josimovich, Isabella M.G. Bukovich, Lauren A. Nazarian, Melia G. Nafus, Bryan M. Kluever, Amy A. Yackel Adams

United States Department of Agriculture Wildlife Services: Staff Publications

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and …

Effective Gamification Within Educational Virtual Reality Environments, Adrian Pilkington

Honors Program: Senior Projects (Public)

With the advent and now widespread use of mobile phones and computers in educational settings, exploring effective and new ways of using technology in the classroom is important for educating the next generation of students. Innovative technology, like Virtual Reality, has started being discussed and researched for its potential benefits in educational settings. This paper presents a brief history of VR, game theory, and examines two VR educational games from the University of Nebraska-Lincoln College of Nursing’s (UNMC) Senior Design teams. The two games will be used to replace lecture-style classes and to go out and educate the community. We …

S Is For Sandhill: A Crane Alphabet, Paul A. Johnsgard

Zea E-Books Collection

This is a book of cranes, from A to Z, written and illustrated by the world’s foremost authority on the 15 species of these wonderful and ancient birds. It is a book for all ages, and for all who love and marvel at the beauty, order, and variety of the natural world.

Cranes exhibit complex behavior, pair-bonding, and fascinating social interactions. They migrate huge distances, crossing continents, oceans, and mountains between their nesting and wintering areas. Seven of the world’s 15 crane species are listed as “vulnerable,” three as “endangered,” one as “critically endangered,” and only three as of “least …

Relationships Among Intramammary Health, Udder And Teat Characteristics, And Productivity Of Extensively Managed Ewes, Ryan M. Knuth, Whitney C. Stewart, Joshua B. Taylor, Bledar Bisha, Carl J. Yeoman, Megan L. Van Emon, Thomas W. Murphy

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Mastitis is an economically important disease and its subclinical state is difficult to diagnose, which makes mitigation more challenging. The objectives of this study were to screen clinically healthy ewes in order to 1) identify cultivable microbial species in milk, 2) evaluate somatic cell count (SCC) thresholds associated with intramammary infection, and 3) estimate relationships between udder and teat morphometric traits, SCC, and ewe productivity. Milk was collected from two flocks in early (<5 >d) and peak (30 to 45 d) lactation to quantify SCC (n = 530) and numerate cultivable microbial species by culture-based isolation followed by matrix-assisted laser …

Wpa News 114 (2021), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Spring 2021), number 114

Published by the World Pheasant Association

Locus Of Heat Resistance (Lhr) In Meat-Borne Escherichia Coli: Screening And Genetic Characterization, Manita Guragain, Dayna M. Brichta-Harhay, James L. Bono, Joseph M. Bosilevac

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Microbial resistance to processing treatments poses a food safety concern, as treatment tolerant pathogens can emerge. Occasional foodborne outbreaks caused by pathogenic Escherichia coli have led to human and economic losses. Therefore, this study screened for the extreme heat resistance (XHR) phenotype as well as one known genetic marker, the locus of heat resistance (LHR), in 4,123 E. coli isolates from diverse meat animals at different processing stages. The prevalences of XHR and LHR among the meat-borne E. coli were found to be 10.3% and 11.4%, respectively, with 19% agreement between the two. Finished meat products showed the highest LHR …

Characterization Of The Domestic Goat Γδ T Cell Receptor Gene Loci And Gene Usage, Alexandria Gillespie, Al Yirsaw, Karthick P. Gunasekaran, Timothy P. L. Smith, Derek M. Bickhart, Michael Turley, Timothy Connelley, Janice C. Telfer, Cynthia L. Baldwin

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Goats and cattle diverged 30 million years ago but retain similarities in immune system genes. Here, the caprine T cell receptor (TCR) gene loci and transcription of its genes were examined and compared to cattle. We annotated the TCR loci using an improved genome assembly (ARS1) of a highly homozygous San Clemente goat. This assembly has already proven useful for describing other immune system genes including antibody and leucocyte receptors. Both the TCRγ (TRG) and TCRδ (TRD) loci were similarly organized in goats as in cattle and the gene sequences were highly conserved. However, the number of genes varied slightly …

Music Makers, Michaela Diehm

Honors Program: Expanded Learning Clubs

After school kids club that learns about music notes and incorporates games and dances to help students learn more about music, how to create their own music, and its importance to everyday life.